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[PMID]:28583258
[Au] Autor:Plunkett G; Jacobson RS; Golden DBK
[Ad] Endereço:ALK-Abello Inc, Round Rock, Texas.
[Ti] Título:Hymenoptera venoms used to produce allergen extracts.
[So] Source:Ann Allergy Asthma Immunol;118(6):649-654, 2017 Jun.
[Is] ISSN:1534-4436
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To review the methods and materials used for collection, purification, commercial production, and clinical application of Hymenoptera venoms. DATA SOURCES: Most of the sources for this review are the experience and expertise of the authors. Published reports and review articles on Hymenoptera venom collection and production were identified through database searches (PubMed). STUDY SELECTIONS: Studies describing the methods for Hymenoptera venom collection and production were selected for review. RESULTS: Meticulous methods for identification and collection of the insects are required. Collection and purification of the venoms from the insects are based on validated methods and result in a commercial extract that is standardized for the major allergenic proteins required for accurate diagnosis and safe and effective treatment of patients allergic to insect sting. The steps required for mixing, purifying, testing, and standardizing the products are described. CONCLUSION: Hymenoptera venom extracts were developed using many new methods for the collection, purification, and commercial production of the unique materials required for this product. Clinical applications for diagnosis and treatment are affected by the integrity and stability of the allergens after processing and purification.
[Mh] Termos MeSH primário: Alérgenos/imunologia
Venenos de Artrópodes/imunologia
Produtos Biológicos/imunologia
Dessensibilização Imunológica
Himenópteros
[Mh] Termos MeSH secundário: Alérgenos/história
Animais
Venenos de Artrópodes/história
Produtos Biológicos/história
História do Século XX
História do Século XXI
Seres Humanos
[Pt] Tipo de publicação:HISTORICAL ARTICLE; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 0 (Arthropod Venoms); 0 (Biological Products)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170719
[Lr] Data última revisão:
170719
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170607
[St] Status:MEDLINE


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[PMID]:28477785
[Au] Autor:Khurana T; Bridgewater JL; Rabin RL
[Ad] Endereço:Division of Vaccines and Related Products Applications, US Food and Drug Administration, Silver Spring, Maryland.
[Ti] Título:Allergenic extracts to diagnose and treat sensitivity to insect venoms and inhaled allergens.
[So] Source:Ann Allergy Asthma Immunol;118(5):531-536, 2017 May.
[Is] ISSN:1534-4436
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To review allergenic extracts used to diagnose or treat insect allergies, including how the extracts are manufactured and their measurements of potency or concentration. DATA SOURCES: Peer-reviewed articles derived from searching PubMed (National Center for Biotechnology Information) about insect allergies and extract preparation. Encyclopedia of Life (http://www.eol.org/) and http://allergome.org/ were also referenced for background information on insects and associated allergens. STUDY SELECTIONS: Search terms used for the PubMed searches included insect allergens and allergies, Apidae, Vespidae, fire ants, cockroach allergies, insect allergen extract preparation, and standardization. RESULTS: Humans may be sensitized to insect allergens by inhalation or through stings. Cockroaches and moths are predominantly responsible for inhalation insect allergy and are a major indoor allergen in urban settings. Bees, fire ants, and wasps are responsible for sting allergy. In the United States, there are multiple insect allergen products commercially available that are regulated by the US Food and Drug Administration. Of those extracts, honeybee venom and insect venom proteins are standardized with measurements of potency. The remaining insect allergen extracts are nonstandardized products that do not have potency measurements. CONCLUSION: Sensitization to inhalational and stinging insect allergens is reported worldwide. Crude insect allergen extracts are used for diagnosis and specific immunotherapy. A variety of source materials are used by different manufacturers to prepare these extracts, which may result in qualitative differences that are not reflected in measurements of potency or protein concentration.
[Mh] Termos MeSH primário: Alérgenos/imunologia
Venenos de Artrópodes/imunologia
Dessensibilização Imunológica
Hipersensibilidade/diagnóstico
Hipersensibilidade/terapia
Mordeduras e Picadas de Insetos/diagnóstico
Mordeduras e Picadas de Insetos/terapia
[Mh] Termos MeSH secundário: Alérgenos/isolamento & purificação
Animais
Venenos de Artrópodes/isolamento & purificação
Seres Humanos
Imunização
Inalação
[Pt] Tipo de publicação:JOURNAL ARTICLE; META-ANALYSIS; REVIEW
[Nm] Nome de substância:
0 (Allergens); 0 (Arthropod Venoms)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170804
[Lr] Data última revisão:
170804
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170508
[St] Status:MEDLINE


  3 / 898 MEDLINE  
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Moraes, Roberto Henrique Pinto
Texto completo SciELO Brasil
[PMID]:28443942
[Au] Autor:Sano-Martins IS; Duarte AC; Guerrero B; Moraes RHP; Barros EJG; Arocha-Piñango CL
[Ad] Endereço:Instituto Butantan, Laboratório de Fisiopatologia, São Paulo, São Paulo, Brazil.
[Ti] Título:Hemostatic disorders induced by skin contact with Lonomia obliqua (Lepidoptera, Saturniidae) caterpillars.
[So] Source:Rev Inst Med Trop Sao Paulo;59:e24, 2017 Apr 20.
[Is] ISSN:1678-9946
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Patients envenomed by Lonomia sp caterpillars initially experience a mild burning pain, headache, nausea, vomiting, and skin and mucosal hemorrhages. Some patients can rapidly progress to a severe coagulopathy that presents as visceral or intracerebral hemorrhaging. We studied the hemostatic alterations that occurred in 14 patients who were envenomed by Lonomia obliqua in Southern Brazil and presented at the Hospital São Vicente de Paulo (Passo Fundo, RS), Brazil during the summers of 1993 and 1994 when Lonomia antivenom was not yet available for treatment. The patients were classified into to 4 clinical groups: 0 (two patients), I (eight patients), II (two patients), and III (two patients). The patients were admitted to the hospital between 4 hours and five days after contact with the caterpillars. In this study, the coagulation parameters of the patients were followed up for up to 172 hours after the accidents. The patients received no treatment with the exceptions of two patients who received blood transfusions and antifibrinolytic treatment. The observed abnormalities related to blood coagulation and fibrinolytic factors were similar regardless of the severity of the bleeding symptoms. These findings suggest that alterations in hemostatic parameters without thrombocytopenia are not predictors of the seriousness of such accidents. Thus, consumptive disorder and reactive fibrinolysis are not proportional to mild coagulopathy. Furthermore, these patients recovered. The hemostatic parameters of most of the patients normalized between 96 and 120 h after the accident.
[Mh] Termos MeSH primário: Antivenenos/administração & dosagem
Venenos de Artrópodes/envenenamento
Transtornos Hemostáticos/induzido quimicamente
Lepidópteros/classificação
[Mh] Termos MeSH secundário: Adulto
Idoso
Animais
Criança
Pré-Escolar
Feminino
Transtornos Hemostáticos/prevenção & controle
Seres Humanos
Masculino
Meia-Idade
Índice de Gravidade de Doença
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antivenins); 0 (Arthropod Venoms)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170608
[Lr] Data última revisão:
170608
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170427
[St] Status:MEDLINE


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[PMID]:28247497
[Au] Autor:Wang Y; Li X; Yang M; Wu C; Zou Z; Tang J; Yang X
[Ad] Endereço:Ethic Drug Screening and Pharmacology Center, Key Laboratory of Chemistry in Ethnic Medicine Resource, State Ethnic Affairs Commission and Ministry of Education, Yunnan University of Nationalities, Kunming, 650500, China.
[Ti] Título:Centipede venom peptide SsmTX-I with two intramolecular disulfide bonds shows analgesic activities in animal models.
[So] Source:J Pept Sci;23(5):384-391, 2017 May.
[Is] ISSN:1099-1387
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Pain is a major symptom of many diseases and results in enormous pressures on human body or society. Currently, clinically used analgesic drugs, including opioids and nonsteroidal anti-inflammatory drugs, have adverse reactions, and thus, the development of new types of analgesic drug candidates is urgently needed. Animal venom peptides have proven to have potential as new types of analgesic medicine. In this research, we describe the isolation and characterization of an analgesic peptide from the crude venom of centipede, Scolopendra subspinipes mutilans. The amino acid sequence of this peptide was identical with SsmTX-I that was previously reported as a specific Kv2.1 ion channel blocker. Our results revealed that SsmTX-I was produced by posttranslational processing of a 73-residue prepropeptide. The intramolecular disulfide bridge motifs of SsmTX-I was Cys1-Cys3 and Cys2-Cys4. Functional assay revealed that SsmTX-I showed potential analgesic activities in formalin-induced paw licking, thermal pain, and acetic acid-induced abdominal writhing mice models. Our research provides the first report of cDNA sequences, disulfide motif, successful synthesis, and analgesic potential of SsmTX-I for the development of pain-killing drugs. It indicates that centipede peptide toxins could be a treasure trove for the search of novel analgesic drug candidates. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd.
[Mh] Termos MeSH primário: Analgésicos/síntese química
Venenos de Artrópodes/genética
Artrópodes/metabolismo
Canais de Potássio Shab/antagonistas & inibidores
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Analgésicos/química
Analgésicos/farmacologia
Animais
Venenos de Artrópodes/química
Venenos de Artrópodes/farmacologia
Artrópodes/genética
Clonagem Molecular
Dissulfetos/química
Seres Humanos
Camundongos
Modelos Animais
Manejo da Dor
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Analgesics); 0 (Arthropod Venoms); 0 (Disulfides); 0 (Shab Potassium Channels)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170613
[Lr] Data última revisão:
170613
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170302
[St] Status:MEDLINE
[do] DOI:10.1002/psc.2988


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[PMID]:28234049
[Au] Autor:Calabria CW; Hauswirth DW; Rank M; Sher L; Larenas-Linnemann D
[Ti] Título:American Academy of Asthma, Allergy & Immunology membership experience with venom immunotherapy in chronic medical conditions and pregnancy, and in young children.
[So] Source:Allergy Asthma Proc;38(2):121-129, 2017 Mar 01.
[Is] ISSN:1539-6304
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Few data exist regarding the use of venom immunotherapy (VIT) in specific high-risk chronic medical conditions and pregnancy, and in young children. METHODS: A Web-based survey was sent to American Academy of Asthma Allergy & Immunology members to explore their VIT experience in potential high-risk medical conditions and pregnancy, and in young children. Major problems were defined as "activation of underlying disease and/or VIT not well tolerated (systemic adverse events) and/or VIT discontinued for medical reasons." Results were expressed descriptively. RESULTS: A total of 697 of 5123 surveys (14%) were completed: 87% of the respondents were based in the United States, and 28% worked in an academic setting. Most respondents (71%) believed that pregnancy was a contraindication for starting VIT. Most were comfortable continuing VIT (51%) if the woman became pregnant after starting therapy. Of the allergists who treated children, many would give VIT down to age 5 years (42%) or younger, ages 1-4 years (35%). The following list is of the specific medical condition, the number of allergists who used VIT in patients with this condition, and the percentage who reported major problems: severe asthma, 212 (4.2%); hypertension, 287 (1.1%); coronary artery disease, 222 (3.6%); arrhythmias, 136 (3.4%); cerebrovascular disease, 104 (5.1%); cancer in remission, 166 (0%); cancer stable but still under treatment, 44 (7.2%); a history of bone marrow transplantation, 15 (4.9%); a history of solid organ transplantation, 29 (3.6%); human immunodeficiency virus, 53 (1.4%); acquired immunodeficiency syndrome, 24 (6.2%); stable autoimmune disease, 164 (2.8%); mastocytosis, 66 (18.4%); elevated serum tryptase, 101 (10.8%); immunodeficiency 59 (2.5%). CONCLUSION: Many allergists were comfortable using VIT in young children and continuing but not starting pregnant women on VIT. VIT was commonly used in patients with hypertension, coronary artery disease, arrhythmias, cancer in remission, and stable autoimmune disease. Major problems were most frequently reported in use with mastocytosis, elevated tryptase, and cancer still under treatment.
[Mh] Termos MeSH primário: Anafilaxia/prevenção & controle
Venenos de Artrópodes/uso terapêutico
Atitude do Pessoal de Saúde
Dessensibilização Imunológica/métodos
Hipersensibilidade Imediata/tratamento farmacológico
Padrões de Prática Médica
Complicações na Gravidez/tratamento farmacológico
[Mh] Termos MeSH secundário: Síndrome de Imunodeficiência Adquirida/epidemiologia
Adolescente
Adulto
Fatores Etários
Alergia e Imunologia
Animais
Venenos de Artrópodes/imunologia
Asma/epidemiologia
Doenças Autoimunes/epidemiologia
Transplante de Medula Óssea
Transtornos Cerebrovasculares/epidemiologia
Criança
Pré-Escolar
Doença Crônica
Comorbidade
Doença da Artéria Coronariana/epidemiologia
Feminino
Infecções por HIV/epidemiologia
Seres Humanos
Himenópteros
Hipersensibilidade Imediata/epidemiologia
Hipertensão/epidemiologia
Lactente
Mordeduras e Picadas de Insetos/imunologia
Masculino
Mastocitose/epidemiologia
Neoplasias/epidemiologia
Transplante de Órgãos
Gravidez
Complicações na Gravidez/epidemiologia
Pré-Medicação/utilização
Sociedades Médicas
Inquéritos e Questionários
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arthropod Venoms)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170306
[Lr] Data última revisão:
170306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170225
[St] Status:MEDLINE
[do] DOI:10.2500/aap.2017.38.4024


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[PMID]:28211342
[Au] Autor:Alfaya Arias T; Soriano Gómis V; Soto Mera T; Vega Castro A; Vega Gutiérrez JM; Alonso Llamazares A; Antolín Amérigo D; Carballada Gonzalez FJ; Dominguez Noche C; Gutierrez Fernandez D; Marques Amat L; Martinez Arcediano A; Martinez San Ireneo M; Moreno Ancillo A; Puente Crespo Y; Ruiz Leon B; Sánchez Morillas L; Hymenoptera Allergy Committee of the SEAIC
[Ad] Endereço:Hospital General Universitario de Ciudad Real, Ciudad Real, Spain.
[Ti] Título:Key Issues in Hymenoptera Venom Allergy: An Update.
[So] Source:J Investig Allergol Clin Immunol;27(1):19-31, 2017.
[Is] ISSN:1018-9068
[Cp] País de publicação:Spain
[La] Idioma:eng
[Ab] Resumo:In this review, the Hymenoptera Allergy Committee of the SEAIC analyzes the most recent scientific literature addressing problems related to the diagnosis of hymenoptera allergy and to management of venom immunotherapy. Molecular diagnosis and molecular risk profiles are the key areas addressed. The appearance of new species of hymenoptera that are potentially allergenic in Spain and the associated diagnostic and therapeutic problems are also described. Finally, we analyze the issue of mast cell activation syndrome closely related to hymenoptera allergy, which has become a new diagnostic challenge for allergists given its high prevalence in patients with venom anaphylaxis.
[Mh] Termos MeSH primário: Venenos de Artrópodes/imunologia
Himenópteros/imunologia
Hipersensibilidade/imunologia
Mordeduras e Picadas de Insetos/imunologia
[Mh] Termos MeSH secundário: Animais
Venenos de Artrópodes/uso terapêutico
Hipersensibilidade/diagnóstico
Hipersensibilidade/epidemiologia
Hipersensibilidade/terapia
Testes Imunológicos
Imunoterapia/métodos
Mordeduras e Picadas de Insetos/diagnóstico
Mordeduras e Picadas de Insetos/epidemiologia
Mordeduras e Picadas de Insetos/terapia
Valor Preditivo dos Testes
Fatores de Risco
Índice de Gravidade de Doença
Espanha/epidemiologia
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Arthropod Venoms)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170606
[Lr] Data última revisão:
170606
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170218
[St] Status:MEDLINE
[do] DOI:10.18176/jiaci.0123


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[PMID]:28130397
[Au] Autor:Walker AA; Madio B; Jin J; Undheim EA; Fry BG; King GF
[Ad] Endereço:From the ‡Institute for Molecular Bioscience, a.walker@uq.edu.au glenn.king@imb.uq.edu.au.
[Ti] Título:Melt With This Kiss: Paralyzing and Liquefying Venom of The Assassin Bug (Hemiptera: Reduviidae).
[So] Source:Mol Cell Proteomics;16(4):552-566, 2017 Apr.
[Is] ISSN:1535-9484
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Assassin bugs (Hemiptera: Heteroptera: Reduviidae) are venomous insects, most of which prey on invertebrates. Assassin bug venom has features in common with venoms from other animals, such as paralyzing and lethal activity when injected, and a molecular composition that includes disulfide-rich peptide neurotoxins. Uniquely, this venom also has strong liquefying activity that has been hypothesized to facilitate feeding through the narrow channel of the proboscis-a structure inherited from sap- and phloem-feeding phytophagous hemipterans and adapted during the evolution of Heteroptera into a fang and feeding structure. However, further understanding of the function of assassin bug venom is impeded by the lack of proteomic studies detailing its molecular composition.By using a combined transcriptomic/proteomic approach, we show that the venom proteome of the harpactorine assassin bug includes a complex suite of >100 proteins comprising disulfide-rich peptides, CUB domain proteins, cystatins, putative cytolytic toxins, triabin-like protein, odorant-binding protein, S1 proteases, catabolic enzymes, putative nutrient-binding proteins, plus eight families of proteins without homology to characterized proteins. S1 proteases, CUB domain proteins, putative cytolytic toxins, and other novel proteins in the 10-16-kDa mass range, were the most abundant venom components. Thus, in addition to putative neurotoxins, assassin bug venom includes a high proportion of enzymatic and cytolytic venom components likely to be well suited to tissue liquefaction. Our results also provide insight into the trophic switch to blood-feeding by the kissing bugs (Reduviidae: Triatominae). Although some protein families such as triabins occur in the venoms of both predaceous and blood-feeding reduviids, the composition of venoms produced by these two groups is revealed to differ markedly. These results provide insights into the venom evolution in the insect suborder Heteroptera.
[Mh] Termos MeSH primário: Venenos de Artrópodes/genética
Venenos de Artrópodes/metabolismo
Reduviidae/metabolismo
[Mh] Termos MeSH secundário: Animais
Evolução Molecular
Perfilação da Expressão Gênica/métodos
Filogenia
Proteômica/métodos
Reduviidae/genética
Análise de Sequência de RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arthropod Venoms)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170627
[Lr] Data última revisão:
170627
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170129
[St] Status:MEDLINE
[do] DOI:10.1074/mcp.M116.063321


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[PMID]:28120601
[Au] Autor:Ciccarelli A; Calabrò C; Imperatore C; Scala G
[Ad] Endereço:Allergy Unit, Loreto Crispi Hospital, ASL Napoli 1 - Centro, Naples, Italy.
[Ti] Título:Hymenoptera Venom Allergy. A closer collaboration is needed between allergists and emergency physicians.
[So] Source:Eur Ann Allergy Clin Immunol;49(1):15-17, 2017 Jan.
[Is] ISSN:1764-1489
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:Hymenoptera stings are sometimes fatal in venom-allergic patients. Fatalities mostly occur in previously stung subjects, especially those with a history of systemic reactions, and could be avoided if patients were properly informed of the existence of a prevention strategy for insect stings, referred to an allergy follow-up and prescribed auto-injectable epinephrine and/or venom-specific immunotherapy (VIT). We sought to assess knowledge and awareness of Hymenoptera Venom Allergy (HVA) in a small sample of Emergency Physicians in our geographic area. An eight-point questionnaire on HVA was administered to Emergency Department physicians working in the six largest ED in Naples. Twenty-seven physicians completed the questionnaire. Twenty/27 (74%) were unaware of the classification of Hymenoptera sting reactions, 11/27 (41%) were unaware of the existence of prevention strategies such as VIT, 18/27 (67%) did not refer HVA patients to a specialist follow up. One/27 (4%) prescribed auto-injectable epinephrine and 100% wish better information on the topic. In our survey we found a number of ED physicians whose knowledge of HVA, beyond the emergency treatment, is not satisfactory. A closer collaboration among ED physicians and allergists is urgently needed.
[Mh] Termos MeSH primário: Alergistas
Venenos de Artrópodes/imunologia
Himenópteros/imunologia
Médicos
[Mh] Termos MeSH secundário: Animais
Serviço Hospitalar de Emergência
Seres Humanos
Colaboração Intersetorial
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arthropod Venoms)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170126
[St] Status:MEDLINE


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[PMID]:27986410
[Au] Autor:Cichocka-Jarosz E; Stobiecki M; Brzyski P; Rogatko I; Nittner-Marszalska M; Sztefko K; Czarnobilska E; Lis G; Nowak-Wegrzyn A
[Ad] Endereço:Department of Pediatrics, Jagiellonian University Medical College, Krakow, Poland.
[Ti] Título:Simplification of intradermal skin testing in Hymenoptera venom allergic children.
[So] Source:Ann Allergy Asthma Immunol;118(3):326-332, 2017 Mar.
[Is] ISSN:1534-4436
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The direct comparison between children and adults with Hymenoptera venom anaphylaxis (HVA) has never been extensively reported. Severe HVA with IgE-documented mechanism is the recommendation for venom immunotherapy, regardless of age. OBJECTIVE: To determine the differences in the basic diagnostic profile between children and adults with severe HVA and its practical implications. METHODS: We reviewed the medical records of 91 children and 121 adults. RESULTS: Bee venom allergy was exposure dependent, regardless of age (P < .001). Atopy was more common in children (P = .01), whereas cardiovascular comorbidities were present almost exclusively in adults (P = .001). In the bee venom allergic group, specific IgE levels were significantly higher in children (29.5 kU /L; interquartile range, 11.30-66.30 kU /L) compared with adults (5.10 kU /L; interquartile range, 2.03-8.30 kU /L) (P < .001). Specific IgE levels for culprit insect venom were higher in bee venom allergic children compared with the wasp venom allergic children (P < .001). In adults, intradermal tests revealed higher sensitivity, accompanied by larger area of skin reactions, regardless of type of venom. At concentrations lower than 0.1 µg/mL, 16% of wasp venom allergic children and 39% of bee venom allergic children had positive intradermal test results. The median tryptase level was significantly higher in adults than in children for the entire study group (P = .002), as well as in bee (P = .002) and wasp venom allergic groups (P = .049). CONCLUSION: The basic diagnostic profile in severe HVA reactors is age dependent. Lower skin test reactivity to culprit venom in children may have practical application in starting the intradermal test procedure with higher venom concentrations.
[Mh] Termos MeSH primário: Alérgenos/imunologia
Anafilaxia/diagnóstico
Anafilaxia/imunologia
Venenos de Artrópodes/efeitos adversos
Himenópteros/imunologia
Testes Intradérmicos
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Animais
Venenos de Abelha
Criança
Pré-Escolar
Comorbidade
Feminino
Deformidades Congênitas da Mão
Seres Humanos
Imunoglobulina E/sangue
Imunoglobulina E/imunologia
Mordeduras e Picadas de Insetos
Testes Intradérmicos/efeitos adversos
Testes Intradérmicos/métodos
Masculino
Meia-Idade
Síndrome de Pierre Robin
Estudos Retrospectivos
Venenos de Vespas
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 0 (Arthropod Venoms); 0 (Bee Venoms); 0 (Wasp Venoms); 37341-29-0 (Immunoglobulin E)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170804
[Lr] Data última revisão:
170804
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161218
[St] Status:MEDLINE


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[PMID]:27896920
[Au] Autor:Wang C; Shan B; Wang Q; Xu Q; Zhang H; Lei H
[Ad] Endereço:Department of Pharmacology, Hebei Medical University, Shijiazhuang, China.
[Ti] Título:Fusion of Ssm6a with a protein scaffold retains selectivity on Na 1.7 and improves its therapeutic potential against chronic pain.
[So] Source:Chem Biol Drug Des;89(6):825-833, 2017 Jun.
[Is] ISSN:1747-0285
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Voltage-gated sodium channel Na 1.7 serves as an attractive target for chronic pain treatment. Several venom peptides were found to selectively inhibit Na 1.7 but with intrinsic problems. Among them, Ssm6a, a recently discovered centipede venom peptide, shows the greatest selectivity against Na 1.7, but dissociates from the target too fast and loses bioactivity in synthetic forms. As a disulfide-rich venom peptide, it is difficult to optimize Ssm6a by artificial mutagenesis and produce the peptide with common industrial manufacturing methods. Here, we developed a novel protein scaffold fusion strategy to address these concerns. Instead of directly mutating Ssm6a, we genetically fused Ssm6a with a protein scaffold engineered from human muscle fatty acid-binding protein. The resultant fusion protein, SP-TOX, maintained the selectivity and potency of Ssm6a upon Na 1.7 but dissociated from target at least 10 times more slowly. SP-TOX dramatically reduced inflammatory pain in a rat model through DRG-targeted delivery. Importantly, SP-TOX can be expressed cytosolically in Escherichia coli and purified in a cost-effective way. In summary, our study provided the first example of cytosolically expressed fusion protein with high potency and selectivity on Na 1.7. Our protein scaffold fusion approach may have its broad application in optimizing disulfide-rich venom peptides for therapeutic usage.
[Mh] Termos MeSH primário: Dor Crônica/terapia
Descoberta de Drogas
Proteínas de Ligação a Ácido Graxo/metabolismo
Canal de Sódio Disparado por Voltagem NAV1.7/metabolismo
Peptídeos/metabolismo
Peptídeos/uso terapêutico
Proteínas Recombinantes de Fusão/uso terapêutico
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Venenos de Artrópodes/química
Venenos de Artrópodes/genética
Venenos de Artrópodes/metabolismo
Modelos Animais de Doenças
Seres Humanos
Inflamação/tratamento farmacológico
Canal de Sódio Disparado por Voltagem NAV1.7/química
Peptídeos/química
Peptídeos/genética
Ratos
Proteínas Recombinantes de Fusão/metabolismo
[Pt] Tipo de publicação:EDITORIAL
[Nm] Nome de substância:
0 (Arthropod Venoms); 0 (Fatty Acid-Binding Proteins); 0 (NAV1.7 Voltage-Gated Sodium Channel); 0 (Peptides); 0 (Recombinant Fusion Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161130
[St] Status:MEDLINE
[do] DOI:10.1111/cbdd.12915



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