Base de dados : MEDLINE
Pesquisa : D20.888.065.115 [Categoria DeCS]
Referências encontradas : 2467 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 247 ir para página                         

  1 / 2467 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29322776
[Au] Autor:Zhao Y; Zhang J; Chen Y; Li Z; Nie H; Peng W; Su S
[Ad] Endereço:College of Bee Sciences/College of Life Sciences, Fujian Agriculture and Forestry University , Fuzhou, Fujian 350002, People's Republic of China.
[Ti] Título:Altered Serum Metabolite Profiling and Relevant Pathway Analysis in Rats Stimulated by Honeybee Venom: New Insight into Allergy to Honeybee Venom.
[So] Source:J Agric Food Chem;66(4):871-880, 2018 Jan 31.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To improve our understanding of the disturbed metabolic pathways and cellular responses triggered by honeybee venom stimulation, we compared the changes in serum metabolites in rats, either stimulated or not by honeybee venom, by performing H nuclear magnetic resonance (NMR) spectrometry-based metabonomics to identify potential biomarkers. In this study, 65 metabolites were structurally confirmed and quantified and the following results were obtained. First, by pattern recognition analysis, 14 metabolites were selected as potential biomarkers 3 h after venom stimulation. Second, metabolic pathway analysis showed that methane metabolism, glyoxylate and dicarboxylate metabolism, tricarboxylic acid cycle, glycine, serine, and threonine metabolism, arginine and proline metabolism were affected. Finally, the time-dependent metabolic modifications indicated that rats could recover without medical treatment 24 h after venom stimulation. In summary, this new insight into the changes in serum metabolites in rats after honeybee venom stimulation has enhanced our understanding of the response of an organism to honeybee venom.
[Mh] Termos MeSH primário: Venenos de Abelha/imunologia
Venenos de Abelha/farmacologia
Hipersensibilidade/sangue
Metabolômica/métodos
[Mh] Termos MeSH secundário: Animais
Biomarcadores/sangue
Espectroscopia de Ressonância Magnética
Masculino
Metaboloma
Ratos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bee Venoms); 0 (Biomarkers)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04160


  2 / 2467 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28950273
[Au] Autor:Rosman Y; Confino-Cohen R; Goldberg A
[Ad] Endereço:Allergy and Clinical Immunology Unit, Meir Medical Center, Kfar Saba, Israel.
[Ti] Título:Venom Immunotherapy in High-Risk Patients: The Advantage of the Rush Build-Up Protocol.
[So] Source:Int Arch Allergy Immunol;174(1):45-51, 2017.
[Is] ISSN:1423-0097
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Venom immunotherapy (VIT) is considered to be the gold standard treatment for patients with hymenoptera venom allergy. This treatment induces systemic reactions (SR) in a significant number of patients. OBJECTIVE: To evaluate the outcome of VIT in patients with known risk factors for VIT-induced SR and to compare rush VIT (RVIT) and conventional VIT (CVIT). METHODS: All of the patients who received VIT and had at least one of the following risk factors were included: current cardiovascular disease, uncontrolled asthma, high basal serum tryptase, current treatment with ß-blockers or angiotensin-converting enzyme inhibitors, and age >70 or <5 years. RESULTS: Sixty-four patients were included, and most of them (52; 81.5%) were allergic exclusively to bee venom. Thirty-five (54.7%) patients underwent RVIT and 29 CVIT. The incidence of patients who developed SR during the build-up phase was similar for RVIT and CVIT (25.7 and 27.5%, respectively; p = 1). However, the incidence of SR per injection was significantly higher in CVIT than in RVIT (5.6 and 2.75%, respectively; p = 0.01). Most reactions (79.1%) were mild, limited to the skin. Most of the patients (92.1%) reached the full maintenance dose of 100 µg. This dose was reached by a significantly larger number of patients receiving RVIT compared to CVIT (100 and 82.7%, respectively; p = 0.01). None of the patients experienced exacerbation of their concurrent chronic disease during VIT. CONCLUSION: VIT can be performed safely and efficiently in patients with risk factors for immunotherapy. In these patients RVIT appears to be safer and more efficient than CVIT.
[Mh] Termos MeSH primário: Alérgenos/administração & dosagem
Venenos de Abelha/administração & dosagem
Dessensibilização Imunológica/métodos
Venenos de Vespas/administração & dosagem
[Mh] Termos MeSH secundário: Adulto
Idoso
Animais
Venenos de Abelha/imunologia
Pré-Escolar
Feminino
Seres Humanos
Himenópteros/imunologia
Masculino
Estudos Retrospectivos
Venenos de Vespas/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 0 (Bee Venoms); 0 (Wasp Venoms)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170927
[St] Status:MEDLINE
[do] DOI:10.1159/000479692


  3 / 2467 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28712480
[Au] Autor:Nascimento de Souza R; Silva FK; Alves de Medeiros M
[Ad] Endereço:Department of Physiological Sciences, Federal Rural University of Rio de Janeiro (UFRRJ), Seropedica, RJ, Brazil.
[Ti] Título:Bee Venom Acupuncture Reduces Interleukin-6, Increases Interleukin-10, and Induces Locomotor Recovery in a Model of Spinal Cord Compression.
[So] Source:J Acupunct Meridian Stud;10(3):204-210, 2017 Jun.
[Is] ISSN:2093-8152
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:Spinal cord injuries (SCIs) initiate a series of molecular and cellular events in which inflammatory responses can lead to major neurological dysfunctions. The present study aims to investigate whether bee venom (BV) acupuncture applied at acupoints ST36 (Zusanli) and GV3 (Yaoyangquan) could minimize locomotor deficits and the magnitude of neural tissue losses, and change the balance between pro- and anti-inflammatory cytokines after an SCI by compression. Wistar rats were subjected to an SCI model by compression in which a 2-French Fogarty embolectomy catheter was inflated in the extradural space. The effects of BV acupuncture, in which 20 µL of BV diluted in saline (0.08 mg/kg) was injected at acupoints GV3 and ST36 [BV(ST36+GV3)-SCI] was compared with BV injected at nonacupoints [BV(NP)-SCI] and with no treatment [group subjected only to SCI (CTL-SCI)]. The BV(ST36+GV3)-SCI group showed a significant improvement in the locomotor performance and a decrease of lesion size compared with the controls. BV acupuncture at the ST36 + GV3 increased the expression of interleukin-10 (anti-inflammatory) at 6 hours and reduced the expression of interleukin-6 (proinflammatory) at 24 hours after SCI compared with the controls. Our results suggest that BV acupuncture can reduce neuroinflammation and induce recovery in the SCI compression model.
[Mh] Termos MeSH primário: Terapia por Acupuntura/métodos
Venenos de Abelha/administração & dosagem
Interleucina-10/imunologia
Interleucina-6/imunologia
Compressão da Medula Espinal/imunologia
Compressão da Medula Espinal/terapia
[Mh] Termos MeSH secundário: Pontos de Acupuntura
Animais
Interleucina-10/metabolismo
Interleucina-6/metabolismo
Masculino
Distribuição Aleatória
Ratos
Ratos Wistar
Compressão da Medula Espinal/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bee Venoms); 0 (IL10 protein, human); 0 (IL6 protein, human); 0 (Interleukin-6); 130068-27-8 (Interleukin-10)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170830
[Lr] Data última revisão:
170830
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170718
[St] Status:MEDLINE


  4 / 2467 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
[PMID]:28591253
[Au] Autor:Silva GBD; Vasconcelos AG; Rocha AMT; Vasconcelos VR; Barros J; Fujishima JS; Ferreira NB; Barros EJG; Daher EF
[Ad] Endereço:Universidade de Fortaleza, Faculdade de Medicina, Centro de Ciências da Saúde, Fortaleza, Ceará, Brazil.
[Ti] Título:Acute kidney injury complicating bee stings - a review.
[So] Source:Rev Inst Med Trop Sao Paulo;59:e25, 2017 Jun 01.
[Is] ISSN:1678-9946
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Bee stings can cause severe reactions and have caused many victims in the last years. Allergic reactions can be triggered by a single sting and the greater the number of stings, the worse the prognosis. The poisoning effects can be systemic and can eventually cause death. The poison components are melitin, apamin, peptide 401, phospholipase A2, hyaluronidase, histamine, dopamine, and norepinephrine, with melitin being the main lethal component. Acute kidney injury (AKI) can be observed in patients suffering from bee stings and this is due to multiple factors, such as intravascular hemolysis, rhabdomyolysis, hypotension and direct toxicity of the venom components to the renal tubules. Arterial hypotension plays an important role in this type of AKI, leading to ischemic renal lesion. The most commonly identified biopsy finding in these cases is acute tubular necrosis, which can occur due to both, ischemic injury and the nephrotoxicity of venom components. Hemolysis and rhabdomyolysis reported in many cases in the literature, were demonstrated by elevated serum levels of indirect bilirubin and creatine kinase. The severity of AKI seems to be associated with the number of stings, since creatinine levels were higher, in most cases, when there were more than 1,000 stings. The aim of this study is to present an updated review of AKI associated with bee stings, including the currently advised clinical approach.
[Mh] Termos MeSH primário: Lesão Renal Aguda/etiologia
Venenos de Abelha/envenenamento
Abelhas
Mordeduras e Picadas de Insetos/complicações
[Mh] Termos MeSH secundário: Lesão Renal Aguda/fisiopatologia
Lesão Renal Aguda/terapia
Animais
Venenos de Abelha/química
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Bee Venoms)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170705
[Lr] Data última revisão:
170705
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170608
[St] Status:MEDLINE


  5 / 2467 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28501976
[Au] Autor:Tomsitz D; Brockow K
[Ad] Endereço:Department of Dermatology and Allergy Biederstein, Technische Universität München, Biedersteiner Straße 29, 80802, Munich, Germany.
[Ti] Título:Component Resolved Diagnosis in Hymenoptera Anaphylaxis.
[So] Source:Curr Allergy Asthma Rep;17(6):38, 2017 Jun.
[Is] ISSN:1534-6315
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE OF REVIEW: Hymenoptera anaphylaxis is one of the leading causes of severe allergic reactions and can be fatal. Venom-specific immunotherapy (VIT) can prevent a life-threatening reaction; however, confirmation of an allergy to a Hymenoptera venom is a prerequisite before starting such a treatment. Component resolved diagnostics (CRD) have helped to better identify the responsible allergen. RECENT FINDINGS: Many new insect venom allergens have been identified within the last few years. Commercially available recombinant allergens offer new diagnostic tools for detecting sensitivity to insect venoms. Additional added sensitivity to nearly 95% was introduced by spiking yellow jacket venom (YJV) extract with Ves v 5. The further value of CRD for sensitivity in YJV and honey bee venom (HBV) allergy is more controversially discussed. Recombinant allergens devoid of cross-reactive carbohydrate determinants often help to identify the culprit venom in patients with double sensitivity to YJV and HBV. CRD identified a group of patients with predominant Api m 10 sensitization, which may be less well protected by VIT, as some treatment extracts are lacking this allergen. The diagnostic gap of previously undetected Hymenoptera allergy has been decreased via production of recombinant allergens. Knowledge of analogies in interspecies proteins and cross-reactive carbohydrate determinants is necessary to distinguish relevant from irrelevant sensitizations.
[Mh] Termos MeSH primário: Alérgenos/imunologia
Anafilaxia/diagnóstico
Venenos de Abelha/imunologia
Himenópteros/imunologia
Venenos de Vespas/imunologia
[Mh] Termos MeSH secundário: Anafilaxia/imunologia
Animais
Reações Cruzadas
Seres Humanos
Imunoglobulina E/imunologia
Mordeduras e Picadas de Insetos/diagnóstico
Mordeduras e Picadas de Insetos/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Allergens); 0 (Bee Venoms); 0 (Wasp Venoms); 37341-29-0 (Immunoglobulin E)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170515
[St] Status:MEDLINE
[do] DOI:10.1007/s11882-017-0707-0


  6 / 2467 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28410965
[Au] Autor:Tsuneoka Y; Irie M; Tanaka Y; Sugimoto T; Kobayashi Y; Kusakabe T; Kato K; Hamaguchi S; Namekata I; Tanaka H
[Ad] Endereço:Department of Pharmacology, Faculty of Pharmaceutical Sciences, Toho University, Funabashi, Chiba 274-8510, Japan; Laboratory of Pharmacology, Faculty of Pharmaceutical Science, Tokyo University of Sciences, Noda, Chiba 278-8510, Japan.
[Ti] Título:Permissive role of reduced inwardly-rectifying potassium current density in the automaticity of the guinea pig pulmonary vein myocardium.
[So] Source:J Pharmacol Sci;133(4):195-202, 2017 Apr.
[Is] ISSN:1347-8648
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The electrophysiological properties underlying the automaticity of the guinea pig pulmonary vein myocardium were studied. About 30% of the isolated pulmonary vein tissue preparations showed spontaneous electrical activity, as shown by glass microelectrode recordings from their myocardial layer. The remaining quiescent preparations had a resting membrane potential less negative than that in the left atria. Blockade of the acetylcholine activated potassium current (I ) by tertiapin induced a depolarizing shift of the resting membrane potential and automatic electrical activity in the pulmonary vein, but not in the atria. The tertiapin-induced electrical activity, as well as the spontaneous activity, was inhibited by the application of carbachol or by chelation of intracellular Ca by BAPTA. The isolated pulmonary vein cardiomyocytes had an I density similar to that of the atrial cardiomyocytes, but a lower density of the inwardly-rectifying potassium current (I ). Spontaneous Ca transients were observed in about 30% of the isolated pulmonary vein cardiomyocytes, but not in atrial cardiomyocytes. The Ca transients in the pulmonary vein cardiomyocytes were induced by tertiapin and inhibited by carbachol. These results indicate that the pulmonary vein cardiomyocytes have a reduced density of the inwardly-rectifying potassium current, which plays a permissive role in their intracellular Ca -dependent automaticity.
[Mh] Termos MeSH primário: Cálcio/metabolismo
Miócitos Cardíacos/metabolismo
Potássio/metabolismo
Potássio/fisiologia
Veias Pulmonares/metabolismo
Veias Pulmonares/fisiologia
[Mh] Termos MeSH secundário: Acetilcolina/antagonistas & inibidores
Acetilcolina/farmacologia
Potenciais de Ação/efeitos dos fármacos
Animais
Venenos de Abelha/antagonistas & inibidores
Venenos de Abelha/farmacologia
Carbacol/farmacologia
Ácido Egtázico/análogos & derivados
Ácido Egtázico/farmacologia
Fenômenos Eletrofisiológicos/efeitos dos fármacos
Cobaias
Técnicas In Vitro
Potenciais da Membrana/efeitos dos fármacos
Microscopia Confocal
Bloqueadores dos Canais de Potássio/farmacologia
Veias Pulmonares/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bee Venoms); 0 (Potassium Channel Blockers); 3A7MX9B7E8 (tertiapin); 526U7A2651 (Egtazic Acid); 8Y164V895Y (Carbachol); K22DDW77C0 (1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid); N9YNS0M02X (Acetylcholine); RWP5GA015D (Potassium); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170416
[St] Status:MEDLINE


  7 / 2467 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28398208
[Au] Autor:Carballo I; Carballada F; Nuñez-Orjales R; Martín-Lázaro J; Vidal C; Gonzalez-Quintela A
[Ad] Endereço:Department of Internal Medicine, Hospital and University of Santiago de Compostela, Santiago de Compostela, Spain.
[Ti] Título:Total and Honeybee Venom-Specific Serum IgG4 and IgE in Beekeepers.
[So] Source:J Investig Allergol Clin Immunol;27(2):146-148, 2017.
[Is] ISSN:1018-9068
[Cp] País de publicação:Spain
[La] Idioma:eng
[Mh] Termos MeSH primário: Venenos de Abelha/imunologia
Criação de Abelhas
Abelhas/imunologia
Hipersensibilidade/imunologia
Imunoglobulina E/sangue
Imunoglobulina G/sangue
Mordeduras e Picadas de Insetos/imunologia
Exposição Ocupacional/efeitos adversos
Ocupações
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Animais
Venenos de Abelha/efeitos adversos
Biomarcadores/sangue
Estudos de Casos e Controles
Feminino
Seres Humanos
Hipersensibilidade/sangue
Hipersensibilidade/prevenção & controle
Tolerância Imunológica
Mordeduras e Picadas de Insetos/sangue
Masculino
Meia-Idade
Testes Sorológicos
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bee Venoms); 0 (Biomarkers); 0 (Immunoglobulin G); 37341-29-0 (Immunoglobulin E)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170613
[Lr] Data última revisão:
170613
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170412
[St] Status:MEDLINE
[do] DOI:10.18176/jiaci.0141


  8 / 2467 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28380475
[Au] Autor:Fiedler C; Miehe U; Treudler R; Kiess W; Prenzel F
[Ad] Endereço:Division of Pediatric Pneumology and Allergy, University Hospital for Children and Adolescents, Leipzig, Germany.
[Ti] Título:Long-Term Follow-Up of Children after Venom Immunotherapy: Low Adherence to Anaphylaxis Guidelines.
[So] Source:Int Arch Allergy Immunol;172(3):167-172, 2017.
[Is] ISSN:1423-0097
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Data on the long-term outcome of children after specific venom immunotherapy (VIT) are limited. Therefore, we assessed sting recurrence and anaphylaxis relapse rates as well as adherence to anaphylaxis guidelines with regard to the availability of emergency equipment and education status. METHODS: For this long-term survey, data of 311 children with a history of anaphylactic reactions to hymenoptera stings were collected by chart review. We included patients who were treated with a 3-year VIT between 1993 and 2009 and had completed a questionnaire. RESULTS: Forty of the 311 patients were included. Mean VIT duration was 3.1 years. Of the 40 patients included, 29 children (72.5%) received VIT with vespid venom, 9 with bee venom, and 2 patients with both venoms. During a mean follow-up period of 13 years, 20/40 patients (50%) suffered re-stings. Six of the 20 (30%) patients developed again anaphylactic symptoms (grade 1 n = 5, grade 3 n = 1); 2 were allergic to vespid and 4 to bee venom. Of the entire cohort, only 5/40 (12.5%) had appropriate emergency kits according to the guidelines of the European Academy of Allergy and Clinical Immunology. Among the patients who had emergency kits available, one third (5/15) felt uncertain about the correct application of the medication. Less than two thirds of our population (25/40) affirmed that they have been educated in emergency management. The vast majority (95%; 38/40) of our patients did not have allergy follow-ups after VIT completion. CONCLUSIONS: Anaphylactic relapses are not uncommon, and there are considerable deficits in the emergency management of patients. Hence, comprehensive standardized anaphylaxis education programs as well as regular follow-ups of the allergy status are crucial.
[Mh] Termos MeSH primário: Anafilaxia/prevenção & controle
Venenos de Abelha/imunologia
Dessensibilização Imunológica
Venenos de Vespas/imunologia
[Mh] Termos MeSH secundário: Adolescente
Anafilaxia/etiologia
Animais
Criança
Pré-Escolar
Feminino
Seres Humanos
Imunoglobulina E/sangue
Mordeduras e Picadas de Insetos/sangue
Mordeduras e Picadas de Insetos/complicações
Mordeduras e Picadas de Insetos/imunologia
Masculino
Cooperação do Paciente
Educação de Pacientes como Assunto
Guias de Prática Clínica como Assunto
Recidiva
Inquéritos e Questionários
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bee Venoms); 0 (Wasp Venoms); 37341-29-0 (Immunoglobulin E)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170406
[St] Status:MEDLINE
[do] DOI:10.1159/000458707


  9 / 2467 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28320163
[Au] Autor:Zhang Y; Huang Y; Wang G; Wang X; Wang Y
[Ad] Endereço:Institutes of Brain Science, State Key Laboratory for Medical Neurobiology, Collaborative Innovation Center for Brain Science, Department of Neurology, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
[Ti] Título:Inhibition of 17-beta-estradiol on neuronal excitability via enhancing GIRK1-mediated inwardly rectifying potassium currents and GIRK1 expression.
[So] Source:J Neurol Sci;375:335-341, 2017 Apr 15.
[Is] ISSN:1878-5883
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Catamenial epilepsy is a common central nervous system disease in female, which is influenced by the 17-ß-estradiol (estrogen) level during the menstrual cycle. Low level (<0.05ng/ml) of estrogen normally accompanies with the perimenstrual classification of catamenial epilepsy, however, without clear mechanism. In previous studies, estrogen has been demonstrated to possess widely regulatory effects on potassium channels. Here, the effect of 17-ß-estradiol on modulating inwardly rectifying K (Kir) currents was investigated in cultured hippocampal neurons. The underlying mechanism was also detected. METHODS: In this research, null-estrogen cultures and spaying animals were used to mimicked the low level estrogen condition in menstrual period. Patch clamp recordings, western blotting and pharmacological experiments were performed to detect the effects of estrogen receptors and the underlying mechanisms. RESULTS: Compared to those neurons in normal medium (with 0.1ng/ml estrogen), null-estrogen cultures or neurons treated by estrogen receptor blocker (ICI 182,780) both had significant suppressed Kir currents. The expression level of G protein-gated inwardly rectifying K channel subunit 1 (GIRK1) was significantly decreased in spaying animals. Furthermore, a GIRK channel inhibitor (TPQ) similarly suppressed the Kir currents. Lastly, estrogen deficiency, estrogen receptor blocker and GIRK channel inhibitor all promoted the epileptiform bursting activities in neurons, as a result of Kir current suppression. CONCLUSION: Taken together, 17-ß-estradiol, by the activation of estrogen receptors, is essential for the maintenance of Kir currents, and thus has an inhibitory effect on the epileptiform bursting activities in cultured hippocampal neurons, whereas GIRK1 is the major intermedial mediator. This research provides a new mechanism for the pathogenesis of catamenial epilepsy, particularly in the menstrual period and the early section of follicular phase.
[Mh] Termos MeSH primário: Estradiol/farmacologia
Canais de Potássio Corretores do Fluxo de Internalização Acoplados a Proteínas G/genética
Regulação da Expressão Gênica/efeitos dos fármacos
Potenciais da Membrana/efeitos dos fármacos
Neurônios/efeitos dos fármacos
Canais de Potássio Corretores do Fluxo de Internalização/metabolismo
[Mh] Termos MeSH secundário: Animais
Venenos de Abelha/farmacologia
Células Cultivadas
Modelos Animais de Doenças
Embrião de Mamíferos
Epilepsia/induzido quimicamente
Epilepsia/tratamento farmacológico
Epilepsia/metabolismo
Estradiol/análogos & derivados
Antagonistas do Receptor de Estrogênio/farmacologia
Feminino
Canais de Potássio Corretores do Fluxo de Internalização Acoplados a Proteínas G/metabolismo
Hipocampo/citologia
Ovariectomia
Bloqueadores dos Canais de Potássio/farmacologia
Gravidez
Ratos
Ratos Sprague-Dawley
Bloqueadores dos Canais de Sódio/farmacologia
Tetrodotoxina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bee Venoms); 0 (Estrogen Receptor Antagonists); 0 (G Protein-Coupled Inwardly-Rectifying Potassium Channels); 0 (Potassium Channel Blockers); 0 (Potassium Channels, Inwardly Rectifying); 0 (Sodium Channel Blockers); 22X328QOC4 (fulvestrant); 3A7MX9B7E8 (tertiapin); 4368-28-9 (Tetrodotoxin); 4TI98Z838E (Estradiol)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170705
[Lr] Data última revisão:
170705
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE


  10 / 2467 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28219072
[Au] Autor:Movérare R; Blume K; Lind P; Crevel R; Marknell DeWitt Å; Cochrane S
[Ad] Endereço:Thermo Fisher Scientific, ImmunoDiagnostics, Uppsala University, Uppsala, Sweden.
[Ti] Título:Human Allergen-Specific IgG Subclass Antibodies Measured Using ImmunoCAP Technology.
[So] Source:Int Arch Allergy Immunol;172(1):1-10, 2017.
[Is] ISSN:1423-0097
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Knowledge of human IgG subclass antibody responses to various allergens has been hampered by a lack of reliable standardized assays. The aim here was to develop quantitative immunoassays for human IgG1, IgG2, and IgG3 antibodies using ImmunoCAP® technology and to evaluate their application. METHODS: Enzyme conjugates with isotype-specific monoclonal antibodies and calibrators composed of purified myeloma paraproteins were developed for each assay and used together with other standardized assay reagents for the Phadia® 100 instrument. The calibrators were adjusted to the international reference preparation IRP 67/86. The assays were characterized and used together with other standard ImmunoCAP assays to measure antibodies to various allergens in preliminary studies. RESULTS: The new assays had limits of quantitation of 1.0 (IgG1), 4.6 (IgG2), and 0.04 mgA/L (IgG3), and coefficients of variation of <20%. Only some minor cross-reactivity with IgG2 was observed for the specific IgG1 assay. The specific IgG2 assay showed a bias for the allotype G2m(23) and compensation factors were used to adjust the measured concentrations accordingly. Preliminary studies indicated a strong and stable IgG4 antibody response to ß-lactoglobulin in healthy individuals, a high IgG1 and even higher IgG2 antibody response to house dust mite in sensitized and nonsensitized subjects, and a mixed IgG subclass response to venom allergens in allergic patients with increasing IgG4 antibody levels during venom immunotherapy. CONCLUSIONS: The new research assays are valuable tools for immunological studies, enabling the characterization of antibody profiles using a standardized approach, and facilitating data interpretation and the comparison of results across studies.
[Mh] Termos MeSH primário: Alérgenos/imunologia
Especificidade de Anticorpos/imunologia
Imunoensaio/métodos
Imunoglobulina G/análise
Isotipos de Imunoglobulinas/análise
[Mh] Termos MeSH secundário: Adulto
Animais
Anticorpos Monoclonais/imunologia
Venenos de Abelha/imunologia
Seres Humanos
Imunoglobulina G/classificação
Imunoglobulina G/imunologia
Alótipos da Imunoglobulina Gm/imunologia
Isotipos de Imunoglobulinas/imunologia
Lactoglobulinas/imunologia
Pyroglyphidae/imunologia
Venenos de Vespas/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 0 (Antibodies, Monoclonal); 0 (Bee Venoms); 0 (Immunoglobulin G); 0 (Immunoglobulin Gm Allotypes); 0 (Immunoglobulin Isotypes); 0 (Lactoglobulins); 0 (Wasp Venoms)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170327
[Lr] Data última revisão:
170327
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170221
[St] Status:MEDLINE
[do] DOI:10.1159/000455098



página 1 de 247 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde