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Pesquisa : D23.050.063 [Categoria DeCS]
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[PMID]:29337391
[Au] Autor:Ishii T; Niikura Y; Kurata K; Muroi M; Tanamoto K; Nagase T; Sakaguchi M; Yamashita N
[Ad] Endereço:Department of Pharmacotherapy, Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo, Japan.
[Ti] Título:Time-dependent distinct roles of Toll-like receptor 4 in a house dust mite-induced asthma mouse model.
[So] Source:Scand J Immunol;87(3), 2018 Mar.
[Is] ISSN:1365-3083
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:House dust mites (HDMs) are a common source of allergens that trigger both allergen-specific and innate immune responses in humans. Here, we examined the effect of allergen concentration and the involvement of Toll-like receptor 4 (TLR4) in the process of sensitization to house dust mite allergens in an HDM extract-induced asthma mouse model. Intranasal administration of HDM extract induced an immunoglobulin E response and eosinophilic inflammation in a dose-dependent manner from 2.5 to 30 µg/dose. In TLR4-knockout mice, the infiltration of eosinophils and neutrophils into the lung was decreased compared with that in wild-type mice in the early phase of inflammation (total of three doses). However, in the late phase of inflammation (total of seven doses), eosinophil infiltration was significantly greater in TLR4-knockout mice than in wild-type mice. This suggests that the roles of TLR4 signaling are different between the early phase and the later phase of HDM allergen-induced inflammation. Thus, innate immune response through TLR4 regulated the response to HDM allergens, and the regulation was altered during the phase of inflammation.
[Mh] Termos MeSH primário: Alérgenos/imunologia
Antígenos de Dermatophagoides/imunologia
Asma/imunologia
Imunidade Inata/imunologia
Pyroglyphidae/imunologia
Receptor 4 Toll-Like/imunologia
[Mh] Termos MeSH secundário: Resistência das Vias Respiratórias/imunologia
Animais
Líquido da Lavagem Broncoalveolar/citologia
Modelos Animais de Doenças
Eosinófilos/patologia
Feminino
Imunização
Imunoglobulina E/imunologia
Inflamação/imunologia
Pulmão/citologia
Pulmão/imunologia
Pulmão/patologia
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Infiltração de Neutrófilos/imunologia
Neutrófilos/patologia
Transdução de Sinais/imunologia
Receptor 4 Toll-Like/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 0 (Antigens, Dermatophagoides); 0 (Tlr4 protein, mouse); 0 (Toll-Like Receptor 4); 37341-29-0 (Immunoglobulin E)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE
[do] DOI:10.1111/sji.12641


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[PMID]:29278026
[Au] Autor:Tokodi M; Csábi E; Kiricsi Á; Kollár E; Molnár AH; Rovó L; Bella Z
[Ad] Endereço:1 Department of Oto-Rhino-Laryngology and Head-Neck Surgery, University of Szeged , Szeged, Hungary.
[Ti] Título:The effect of nasal provocation with a single-dose allergen on the physical and cognitive performance of patients with ragweed allergy.
[So] Source:Physiol Int;104(4):334-343, 2017 Dec 01.
[Is] ISSN:2498-602X
[Cp] País de publicação:Hungary
[La] Idioma:eng
[Ab] Resumo:Purpose This study aims to compare the impact of active allergic rhinitis on physical and cognitive abilities of trained allergic athletes to untrained allergic patients. Methods Cognitive, respiratory, and fitness functions were assessed before and after allergen exposure. Participants in both groups were provoked intranasally with ragweed allergen. Results The group of athletes revealed significantly higher average values in peak inspiratory flow and fitness index before and after provocation. In neuropsychological assessments, athletes performed significantly better after allergen provocation in complex working memory capacity. Due to single acute allergen exposure, the size of the nasal cavity and nasal inspiratory peak flow significantly decreased in both groups. The physical performance of both groups did not change after provocation. Executive functions and complex working memory capacity of athletes significantly improved resulting from provocation. Conclusions A single-shot allergen in high dose might cause an increase in mental concentration, which was more pronounced in the group of athletes. This study indicates that acute exposure to allergen cannot affect the physical performance and may result in increased mental focus in patients with allergy notwithstanding the declining respiratory functions.
[Mh] Termos MeSH primário: Alérgenos/administração & dosagem
Antígenos de Plantas/administração & dosagem
Desempenho Atlético
Cognição/efeitos dos fármacos
Testes de Provocação Nasal/métodos
Extratos Vegetais/administração & dosagem
Desempenho Psicomotor/efeitos dos fármacos
Rinite Alérgica/fisiopatologia
[Mh] Termos MeSH secundário: Adulto
Relação Dose-Resposta a Droga
Esquema de Medicação
Feminino
Seres Humanos
Masculino
Rinite Alérgica/diagnóstico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 0 (Antigens, Plant); 0 (Plant Extracts); 0 (ragweed pollen)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171227
[St] Status:MEDLINE
[do] DOI:10.1556/2060.104.2017.4.6


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[PMID]:29200850
[Au] Autor:Aliu H; Rask C; Brimnes J; Andresen TL
[Ad] Endereço:Immunology Department, In vivo Biology Team, ALK Abelló A/S, Hørsholm.
[Ti] Título:Enhanced efficacy of sublingual immunotherapy by liposome-mediated delivery of allergen.
[So] Source:Int J Nanomedicine;12:8377-8388, 2017.
[Is] ISSN:1178-2013
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:Immunotherapy by sublingual administration of allergens provides high patient compliance and has emerged as an alternative to subcutaneous immunotherapy for the treatment of IgE-associated allergic diseases. However, sublingual immunotherapy (SLIT) can cause adverse events. Development of allergen delivery systems enabling more efficient delivery and hence lower allergen load might reduce the adverse events. In the present study, we have investigated neutral and cationic liposomes as delivery systems of ovalbumin (OVA), as a model allergen, in an OVA-induced allergic airway inflammation model. We investigated the liposome carriers' ability to improve tolerance induction of antigens compared to the corresponding dose of free OVA. Mice were treated sublingually over 2 weeks with free or liposome encapsulated OVA followed by intraperitoneal injections and intranasal challenge. Mice sublingually treated with OVA-liposomes showed a significant reduction of airway eosinophilia and splenocyte proliferation in comparison to free OVA. A similar nonsignificant pattern was seen for OVA-specific IgE antibodies. In addition, reduced levels of interferon-γ and interleukin-5 were observed in spleen cell culture supernatants from OVA-liposome-treated mice compared to the sham-treated group. In conclusion, in vivo efficacy data showed that prophylactic SLIT with OVA-liposomes is significantly more effective in preventing allergic inflammation than the corresponding dose of free OVA.
[Mh] Termos MeSH primário: Alérgenos/administração & dosagem
Sistemas de Liberação de Medicamentos
Imunoterapia Sublingual
[Mh] Termos MeSH secundário: Alérgenos/imunologia
Animais
Citocinas/metabolismo
Ensaio de Imunoadsorção Enzimática
Feminino
Lipídeos/química
Lipossomos
Camundongos Endogâmicos BALB C
Ovalbumina/imunologia
Pneumonia/imunologia
Pneumonia/patologia
Pneumonia/prevenção & controle
Baço/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 0 (Cytokines); 0 (Lipids); 0 (Liposomes); 9006-59-1 (Ovalbumin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE
[do] DOI:10.2147/IJN.S137033


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[PMID]:29437642
[Au] Autor:Davies JM; Thien F; Hew M
[Ad] Endereço:Office of Research, Institute of Health and Biomedical Innovation, Queensland University of Technology, Queensland, Australia, and Metro North Hospital and Health Service, Brisbane, Queensland, Australia.
[Ti] Título:Thunderstorm asthma: controlling (deadly) grass pollen allergy.
[So] Source:BMJ;360:k432, 2018 02 06.
[Is] ISSN:1756-1833
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Asma
Rinite Alérgica Sazonal
[Mh] Termos MeSH secundário: Alérgenos
Seres Humanos
Poaceae/imunologia
Pólen/imunologia
[Pt] Tipo de publicação:LETTER; RESEARCH SUPPORT, NON-U.S. GOV'T; COMMENT
[Nm] Nome de substância:
0 (Allergens)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180214
[St] Status:MEDLINE
[do] DOI:10.1136/bmj.k432


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[PMID]:29274211
[Au] Autor:Jakubas-Zawalska J; Asman M; Solarz K
[Ad] Endereço:Department of Parasitology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice, ul. Jednosci 8, 41-218 Sosnowiec, Poland
[Ti] Título:Sensitization to the storage mites Lepidoglyphus destructor and Tyrophagus putrescentiae (Acari, Sarcoptiformes, Astigmatina) in a suburban population in Southern Poland
[So] Source:Ann Parasitol;63(3):183-188, 2017.
[Is] ISSN:2299-0631
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:Mite infestation of stored products is a serious threat to food safety and public health. These stored product mites are not only serious pests of stored food but also cause allergies in humans. Thirty serum samples from patients living in suburban areas of Upper Silesia (South Poland) were tested for sensitization to two species of storage mites: Lepidoglyphus destructor [LD] and Tyrophagus putrescentiae [TP]. Patient antibodies against particular antigens were identified using anti-human anti-IgE monoclonal antibodies. Fifteen protein fractions from LD gave positive reactions with IgE antibodies and 18 from TP. Seven of the 30 samples showed positive reactions to a protein fraction measuring about 29 kDa from LD and six reacted with a fraction measuring about 25 kDa from TP. These findings may imply the existence of many protein fractions with allergenic properties besides the characterized allergens in the two tested species.
[Mh] Termos MeSH primário: Hipersensibilidade/epidemiologia
Hipersensibilidade/imunologia
Ácaros/imunologia
[Mh] Termos MeSH secundário: Alérgenos/imunologia
Alérgenos/metabolismo
Animais
Feminino
Seres Humanos
Masculino
Ácaros/metabolismo
Polônia/epidemiologia
População Suburbana
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171224
[St] Status:MEDLINE
[do] DOI:10.17420/ap6303.104


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[PMID]:28460633
[Au] Autor:Zhang Z; Zheng W; Xie H; Chai R; Wang J; Zhang H; He S
[Ad] Endereço:Allergy and Clinical Immunology Research Centre, The First Affiliated Hospital of Jinzhou Medical University, No. 2, Section 5, Renmin Street, Guta District, Jinzhou, 121001, Liaoning, People's Republic of China.
[Ti] Título:Up-regulated expression of substance P in CD8 T cells and NK1R on monocytes of atopic dermatitis.
[So] Source:J Transl Med;15(1):93, 2017 May 01.
[Is] ISSN:1479-5876
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Large numbers of CD8 T cells were observed in atopic dermatitis (AD) skin, and monocytes from AD patients showed increased prostaglandin E2 production. However, little is known about the expression of substance P (SP) and its receptor NK1R in blood leukocytes of patients with AD. OBJECTIVE: To explore the expression of SP and NK1R in leukocytes of AD and the influence of allergens on SP and NK1R expression. METHODS: The expression levels of SP and NK1R in patients with AD were examined by flow cytometry, ELISA and a mouse AD model. RESULTS: The plasma SP level was 4.9-fold higher in patients with AD than in HC subjects. Both the percentage of SP expression in the population and mean fluorescence intensity (MFI) of SP expression were elevated in CD8 T cells in the blood of AD patients. However, both the CD14 NK1R population and MFI of NK1R expression on CD14 cells were enhanced in the blood of AD patients. Allergens ASWE, HDME and PPE failed to up-regulate SP expression in CD8 T cells. However, allergens ASWE and HDME both enhanced NK1R expression on CD14 blood leukocytes regardless of AD or HC subjects. OVA-sensitized AD mice showed an elevated proportion and MFI of SP-expressing CD8 T cells in the blood, which agrees with the SP expression situation in human AD blood. Injection of SP into mouse skin did not up-regulate NK1R expression on monocytes. CONCLUSIONS: An elevated plasma SP level, up-regulated expression of SP and NK1R indicate that the SP/NK1R complex is important in the development of AD. Therefore, SP and NK1R antagonist or blocker agents may help to treat patients with AD. Trial registration Registration number: ChiCTR-BOC-16010279; Registration date: Dec., 28, 2016; retrospectively registered.
[Mh] Termos MeSH primário: Linfócitos T CD8-Positivos/imunologia
Dermatite Atópica/genética
Dermatite Atópica/imunologia
Monócitos/patologia
Receptores da Neurocinina-1/metabolismo
Substância P/genética
Regulação para Cima/genética
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Alérgenos/imunologia
Animais
Estudos de Casos e Controles
Dermatite Atópica/sangue
Citometria de Fluxo
Seres Humanos
Camundongos Endogâmicos BALB C
Meia-Idade
Ovalbumina/imunologia
Substância P/sangue
Substância P/metabolismo
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 0 (Receptors, Neurokinin-1); 33507-63-0 (Substance P); 9006-59-1 (Ovalbumin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1186/s12967-017-1196-6


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[PMID]:28459556
[Au] Autor:Braga RC; Alves VM; Muratov EN; Strickland J; Kleinstreuer N; Trospsha A; Andrade CH
[Ad] Endereço:Laboratory for Molecular Modeling and Drug Design, Faculty of Pharmacy, Federal University of Goiás , Goiânia, GO 74605-170, Brazil.
[Ti] Título:Pred-Skin: A Fast and Reliable Web Application to Assess Skin Sensitization Effect of Chemicals.
[So] Source:J Chem Inf Model;57(5):1013-1017, 2017 05 22.
[Is] ISSN:1549-960X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chemically induced skin sensitization is a complex immunological disease with a profound impact on quality of life and working ability. Despite some progress in developing alternative methods for assessing the skin sensitization potential of chemical substances, there is no in vitro test that correlates well with human data. Computational QSAR models provide a rapid screening approach and contribute valuable information for the assessment of chemical toxicity. We describe the development of a freely accessible web-based and mobile application for the identification of potential skin sensitizers. The application is based on previously developed binary QSAR models of skin sensitization potential from human (109 compounds) and murine local lymph node assay (LLNA, 515 compounds) data with good external correct classification rate (0.70-0.81 and 0.72-0.84, respectively). We also included a multiclass skin sensitization potency model based on LLNA data (accuracy ranging between 0.73 and 0.76). When a user evaluates a compound in the web app, the outputs are (i) binary predictions of human and murine skin sensitization potential; (ii) multiclass prediction of murine skin sensitization; and (iii) probability maps illustrating the predicted contribution of chemical fragments. The app is the first tool available that incorporates quantitative structure-activity relationship (QSAR) models based on human data as well as multiclass models for LLNA. The Pred-Skin web app version 1.0 is freely available for the web, iOS, and Android (in development) at the LabMol web portal ( http://labmol.com.br/predskin/ ), in the Apple Store, and on Google Play, respectively. We will continuously update the app as new skin sensitization data and respective models become available.
[Mh] Termos MeSH primário: Alérgenos
Dermatite de Contato
Internet
Pele
Software
[Mh] Termos MeSH secundário: Alérgenos/toxicidade
Animais
Simulação por Computador
Bases de Dados de Compostos Químicos
Seres Humanos
Ensaio Local de Linfonodo
Camundongos
Relação Quantitativa Estrutura-Atividade
Pele/efeitos dos fármacos
Pele/patologia
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Allergens)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jcim.7b00194


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[PMID]:27778095
[Au] Autor:He S; Mou Z; Peng L; Chen J
[Ad] Endereço:Department of Otorhinolaryngology, Shanghai Children's Medical Center, affiliated with Shanghai Jiaotong University School of Medicine, Shanghai, 200127, China.
[Ti] Título:Impacts of meteorological and environmental factors on allergic rhinitis in children.
[So] Source:Int J Biometeorol;61(5):797-806, 2017 May.
[Is] ISSN:1432-1254
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Meteorological and environmental factors influence the pathogenesis of allergic rhinitis (AR). An understanding of the risk factors will facilitate the development of diagnostic and preventative tools for AR children and improve their quality of life. However, research on the impact of these factors on subjective symptoms in AR children remains scarce. This study explored the relationships between subjective symptoms in pollen and dust mite positive AR children, and meteorological and environmental factors. Using a linear mixed effect model, we analyzed the correlations between monthly data on the subjective symptoms of 351 AR children (from the Shanghai Children's Medical Center) and meteorological and environmental factors during 2013. The monthly meteorological and environmental data were provided by the Shanghai Meteorological Service and Shanghai Environmental Protection Bureau. Temperature and humidity were negatively correlated with the subjective symptom score, with a 0.04 point increase observed for every 1 °C decrease in temperature (P < 0.0001) or 10 % decline in humidity (P = 0.0412). The particulate matter (PM) 10 and PM2.5 concentrations were positively correlated with the subjective symptom score, with a 10 µg/m increase in PM10 and PM2.5 yielding a 0.02 (P = 0.0235) and 0.03 (P = 0.0281) increase in the subjective symptom score, respectively. In conclusion, meteorological and environmental factors were correlated with subjective symptoms in AR children. Low temperatures, lower humidity, and high PM10 and PM2.5 concentrations aggravated subjective symptoms in AR children.
[Mh] Termos MeSH primário: Rinite Alérgica/etiologia
[Mh] Termos MeSH secundário: Adolescente
Poluentes Atmosféricos
Alérgenos/imunologia
Animais
Criança
Pré-Escolar
China/epidemiologia
Feminino
Seres Humanos
Umidade
Modelos Lineares
Masculino
Material Particulado
Pólen/imunologia
Pyroglyphidae/imunologia
Rinite Alérgica/epidemiologia
Fatores de Risco
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Air Pollutants); 0 (Allergens); 0 (Particulate Matter)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE
[do] DOI:10.1007/s00484-016-1257-1


  9 / 35739 MEDLINE  
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[PMID]:29363738
[Au] Autor:Willen SM; Rodeghier M; Strunk RC; Bacharier LB; Rosen CL; Kirkham FJ; DeBaun MR; Cohen RT
[Ad] Endereço:Department of Pediatrics, Division of Hematology/Oncology, Vanderbilt-Meharry Center for Excellence in Sickle Cell Disease, Vanderbilt University Medical Center, Nashville, TN, USA.
[Ti] Título:Aeroallergen sensitization predicts acute chest syndrome in children with sickle cell anaemia.
[So] Source:Br J Haematol;180(4):571-577, 2018 02.
[Is] ISSN:1365-2141
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Asthma is associated with higher rates of acute chest syndrome (ACS) and vaso-occlusive pain episodes among children with sickle cell anaemia (SCA). Aeroallergen sensitization is a risk factor for asthma. We hypothesized that aeroallergen sensitization is associated with an increased incidence of hospitalizations for ACS and pain. Participants in a multicentre, longitudinal cohort study, aged 4-18 years with SCA, underwent skin prick testing to ten aeroallergens. ACS and pain episodes were collected from birth until the end of the follow-up period. The number of positive skin tests were tested for associations with prospective rates of ACS and pain. Multivariable models demonstrated additive effects of having positive skin tests on future rates of ACS (incidence rate ratio (IRR) for each positive test 1·23, 95% confidence interval [CI] 1·11-1·36, P < 0·001). Aeroallergen sensitization was not associated with future pain (IRR 1·14, 95%CI 0·97-1·33, P = 0·11). Our study demonstrated that children with SCA and aeroallergen sensitization are at increased risk for future ACS. Future research is needed to determine whether identification of specific sensitizations and allergen avoidance and treatment reduce the risk of ACS for children with SCA.
[Mh] Termos MeSH primário: Síndrome Torácica Aguda/diagnóstico
Síndrome Torácica Aguda/etiologia
Alérgenos/imunologia
Anemia Falciforme/complicações
[Mh] Termos MeSH secundário: Adolescente
Aerossóis
Biomarcadores
Criança
Pré-Escolar
Feminino
Seguimentos
Seres Humanos
Hipersensibilidade/complicações
Hipersensibilidade/imunologia
Imunização
Masculino
Morbidade
Medição da Dor
Prognóstico
Estudos Prospectivos
Testes Cutâneos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Aerosols); 0 (Allergens); 0 (Biomarkers)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE
[do] DOI:10.1111/bjh.15076


  10 / 35739 MEDLINE  
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[PMID]:29293622
[Au] Autor:Ma Y; Dawicki W; Zhang X; Gordon JR
[Ad] Endereço:Division of Respirology, Critical Care and Sleep Medicine, Department of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
[Ti] Título:Contributions of direct versus indirect mechanisms for regulatory dendritic cell suppression of asthmatic allergen-specific IgG1 antibody responses.
[So] Source:PLoS One;13(1):e0190414, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:IL-10-differentiated dendritic cells (DC10) can reverse the asthma phenotype in mice, but how they suppress the asthmatic B cell response is unclear. Herein we assessed the mechanism(s) by which DC10 and DC10-induced Treg affect IgG1 production in asthma. We observed a rapid decline in lung-resident OVA-specific IgG1-secreting B cells on cessation of airway allergen challenge, and intraperitoneal DC10 therapy did not amplify that (p>0.05). It did however increase the loss of IgG1-B cells from the bone marrow (by 45+/-7.2%; p≤0.01) and spleen (by 65+/-17.8%; p≤0.05) over 2 wk. Delivery of OVA-loaded DC10 directly into the airways of asthmatic mice decreased the lung IgG1 B cell response assessed 2 dy later by 33+/-9.7% (p≤0.01), while their co-culture with asthmatic lung cell suspensions reduced the numbers of IgG1-secreting cells by 56.5+/-9.7% (p≤0.01). This effect was dependent on the DC10 carrying intact allergen on their cell surface; DC10 that had phagocytosed and fully processed their allergen were unable to suppress B cell responses, although they did suppress asthmatic Th2 cell responses. We had shown that therapeutic delivery of DC10-induced Treg can effectively suppress asthmatic T and B cell (IgE and IgG1) responses; herein CD4+ cells or Treg from the lungs of DC10-treated OVA-asthmatic mice suppressed in vitro B cell IgG1 production by 52.2+/-8.7% (p≤0.001) or 44.6+/-12.2% (p≤0.05), respectively, but delivery of DC10-induced Treg directly into the airways of asthmatic mice had no discernible impact over 2 dy on the numbers of lung IgG1-secreting cells (p≥0.05). In summary, DC10 treatment down-regulates OVA-specific B cell responses of asthmatic mice. While DC10 that carry intact allergen on their cell surface can dampen this response, DC10-induced Treg are critical for full realization of this outcome. This suggests that infectious tolerance is an essential element in regulatory DC control of the B cell response in allergic asthma.
[Mh] Termos MeSH primário: Alérgenos/imunologia
Asma/imunologia
Imunoglobulina G/imunologia
Linfócitos T Reguladores/imunologia
[Mh] Termos MeSH secundário: Animais
Separação Celular
Ensaio de Imunoadsorção Enzimática
Feminino
Citometria de Fluxo
Camundongos
Camundongos Endogâmicos BALB C
Ovalbumina/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Allergens); 0 (Immunoglobulin G); 9006-59-1 (Ovalbumin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190414



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