Base de dados : MEDLINE
Pesquisa : D23.050.140 [Categoria DeCS]
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[PMID]:22564293
[Au] Autor:Blanco Y; Prieto-Ballesteros O; Gómez MJ; Moreno-Paz M; García-Villadangos M; Rodríguez-Manfredi JA; Cruz-Gil P; Sánchez-Román M; Rivas LA; Parro V
[Ad] Endereço:Departments of Molecular Evolution Planetology and Habitability Instrumentation, Centro de Astrobiología (INTA-CSIC), Carretera de Ajalvir km 4, Torrejón de Ardoz, 28850 Madrid, Spain.
[Ti] Título:Prokaryotic communities and operating metabolisms in the surface and the permafrost of Deception Island (Antarctica).
[So] Source:Environ Microbiol;14(9):2495-510, 2012 Sep.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this study we examined the microbial community composition and operating metabolisms on the surface and in the permafrost of Deception Island, (Antarctica) with an on site antibody microarray biosensor. Samples (down to a depth of 4.2 m) were analysed with LDChip300 (Life Detector Chip), an immunosensor containing more than 300 antibodies targeted to bacterial and archaeal antigens. The immunograms showed positive antigen-antibody reactions in all surface samples (lichens, pyroclasts) and the top layer of the permafrost. The results indicated the presence of exopolysaccharides, bacteria belonging to the Alpha-, Delta- and Gammaproteobacteria, Bacteroidetes, Gram-positive Actinobacteria and Firmicutes, as well as archaeal species, most probably Methanobacterium spp. Positive reactions with antibodies to proteins and peptides revealed the presence of nitrogen fixation (NifHD, GlnB, HscA), methanogenic (McrB), iron homeostasis and iron scavenging (ferritins and DPS proteins) proteins, as well as ABC transporters, which indicated that these processes were operating at the time of sampling. These results were validated with other molecular ecology techniques such as oligonucleotide microarrays, 16S bacterial rRNA gene sequence analysis, aerobic viable counts and microscopy. Molecular ecology results showed a differentiated pattern along the depth of the drill, being the top active layer the most diverse, with Acidobacteria, Actinobacteria, Proteobacteria, Bacteroidetes and the phototrophs Cyanobacteria and Chloroflexi as dominant groups. Actinobacteria and Firmicutes were dominant in depths from 0.5 to 2 m, and Betaproteobacteria from 3 to 4.2 m. The geochemical analysis revealed the presence of low molecular weight organic acids (acetate, formate) which could be used by microorganisms as energy sources for sulfate, nitrate and metal reduction under anaerobic conditions.
[Mh] Termos MeSH primário: Archaea
Bactérias
Biodiversidade
Ilhas
Microbiologia do Solo
[Mh] Termos MeSH secundário: Regiões Antárticas
Antígenos Arqueais/metabolismo
Antígenos de Bactérias/metabolismo
Archaea/classificação
Archaea/genética
Archaea/metabolismo
Bactérias/classificação
Bactérias/genética
Bactérias/metabolismo
Carga Bacteriana
Biomarcadores/análise
Microscopia Eletrônica de Varredura
Filogenia
RNA Ribossômico 16S/genética
Solo/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, Archaeal); 0 (Antigens, Bacterial); 0 (Biomarkers); 0 (RNA, Ribosomal, 16S); 0 (Soil)
[Em] Mês de entrada:1304
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120509
[St] Status:MEDLINE
[do] DOI:10.1111/j.1462-2920.2012.02767.x


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[PMID]:20331799
[Au] Autor:Yamabe K; Maeda H; Kokeguchi S; Soga Y; Meguro M; Naruishi K; Asakawa S; Takashiba S
[Ad] Endereço:Department of Pathophysiology-Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama, Japan.
[Ti] Título:Antigenic group II chaperonin in Methanobrevibacter oralis may cross-react with human chaperonin CCT.
[So] Source:Mol Oral Microbiol;25(2):112-22, 2010 Apr.
[Is] ISSN:2041-1014
[Cp] País de publicação:Denmark
[La] Idioma:eng
[Ab] Resumo:Methanobrevibacter oralis is an archaeal species frequently isolated from sites of severe periodontitis. However, its pathogenic roles remain unclear. Here, we aimed to isolate group II chaperonin from M. oralis and examine its antigenicity. The genes encoding two chaperonin subunits (Cpn-1 and Cpn-2) were cloned from M. oralis using polymerase chain reaction and genome walking procedures. Recombinant proteins Cpn-1 and Cpn-2 were generated, and the reactivities of sera from patients with periodontitis were examined by Western immunoblotting. The open reading frames of Cpn-1 and Cpn-2 genes consisted of 1641 and 1614 base pairs, respectively. Putative ATP-binding domains conserved among the chaperonin family were observed in both genes. The deduced amino acid sequences of the two genes showed 28.8-40.0% identity to each of the subunits of human CCT (CCT1-8). Thirty and 29 of 36 patients' sera reacted with the recombinant Cpn-1 and recombinant Cpn-2, respectively. Western immunoblotting using antiserum against human CCT subunits indicated that anti-CCT3 and anti-CCT8 antibodies recognized recombinant Cpn-1. In addition, anti-CCT1, CCT3, CCT6, and CCT8 antibodies recognized an antigen of approximately 60 kDa in M. oralis. The results suggested that the chaperonin subunits of M. oralis were antigenic molecules that were recognized by periodontitis patients and that may cross-react with human chaperonin CCT.
[Mh] Termos MeSH primário: Antígenos Arqueais/imunologia
Chaperoninas do Grupo II/imunologia
Methanobrevibacter/patogenicidade
Periodontite/imunologia
Periodontite/microbiologia
[Mh] Termos MeSH secundário: Antígenos Arqueais/genética
Chaperonina com TCP-1/genética
Chaperonina com TCP-1/imunologia
Passeio de Cromossomo
Sequência Conservada/imunologia
Reações Cruzadas
DNA Arqueal/análise
Chaperoninas do Grupo II/genética
Interações Hospedeiro-Patógeno
Seres Humanos
Methanobrevibacter/imunologia
Periodontite/sangue
Subunidades Proteicas/genética
Subunidades Proteicas/imunologia
Proteínas Recombinantes/genética
Proteínas Recombinantes/imunologia
Alinhamento de Sequência
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, Archaeal); 0 (DNA, Archaeal); 0 (Protein Subunits); 0 (Recombinant Proteins); EC 3.6.1.- (Chaperonin Containing TCP-1); EC 3.6.1.- (Group II Chaperonins)
[Em] Mês de entrada:1007
[Cu] Atualização por classe:140217
[Lr] Data última revisão:
140217
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:100325
[St] Status:MEDLINE
[do] DOI:10.1111/j.2041-1014.2009.00548.x


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[PMID]:15046568
[Au] Autor:Prieur D; Erauso G; Geslin C; Lucas S; Gaillard M; Bidault A; Mattenet AC; Rouault K; Flament D; Forterre P; Le Romancer M
[Ad] Endereço:Université de Bretagne Occidentale, UMR 6539, IUEM, Technopôle Brest-Iroise, 29270 Plouzané, France. daniel.prieur@univ-brest.fr
[Ti] Título:Genetic elements of Thermococcales.
[So] Source:Biochem Soc Trans;32(Pt 2):184-7, 2004 Apr.
[Is] ISSN:0300-5127
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This minireview summarizes our current knowledge about archaeal genetic elements in the hyperthermophilic order Thermococcales in the phylum Euryarchaeota. This includes recent work on the first virus of Pyrococcus, PAV1, the discovery of novel unique virus morphotypes in hot deep-sea environments, and preliminary observations on novel cryptic plasmids. We also review the work accomplished over the last 5 years in the development of genetic tools for members of the Pyrococcus and Thermococcus genera, mainly in our laboratories.
[Mh] Termos MeSH primário: Genoma Arqueal
Pyrococcus/genética
Thermococcales/genética
Thermococcus/genética
[Mh] Termos MeSH secundário: Antígenos Arqueais/química
DNA Arqueal/ultraestrutura
Vetores Genéticos
Concentração de Íons de Hidrogênio
Microscopia Eletrônica de Transmissão
Compostos Organometálicos/farmacologia
Plasmídeos/metabolismo
Transgenes
Vírus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antigens, Archaeal); 0 (DNA, Archaeal); 0 (Organometallic Compounds); 285PN2K1AO (uranyl acetate)
[Em] Mês de entrada:0412
[Cu] Atualização por classe:121115
[Lr] Data última revisão:
121115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:040330
[St] Status:MEDLINE


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[PMID]:10826786
[Au] Autor:Wasserfallen A; Nölling J; Pfister P; Reeve J; Conway de Macario E
[Ad] Endereço:Mikrobiologisches Institut, Swiss Federal Institute of Technology, Zurich. wasserfallen@micro.biol.ethz.ch
[Ti] Título:Phylogenetic analysis of 18 thermophilic Methanobacterium isolates supports the proposals to create a new genus, Methanothermobacter gen. nov., and to reclassify several isolates in three species, Methanothermobacter thermautotrophicus comb. nov., Methanothermobacter wolfeii comb. nov., and Methanothermobacter marburgensis sp. nov.
[So] Source:Int J Syst Evol Microbiol;50 Pt 1:43-53, 2000 Jan.
[Is] ISSN:1466-5026
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Using a combination of 16S rRNA analysis and antigenic fingerprinting consisting of new and published data, the phylogenetic position of 18 thermophilic isolates currently classified as Methanobacterium species was reinvestigated. The results were verified by independent methods, including, where applicable, plasmid and phage typing. Comparative analysis of 16S rRNA data for 30 strains belonging to the order Methanobacteriales strongly suggested that mesophilic and thermophilic Methanobacterium isolates are distantly related and should be assigned to separate genera. For the thermophilic strains the genus Methanothermobacter was initially proposed by Boone, Whitman and Rouvière. Furthermore, the results support a reclassification of 15 isolates in three species within the proposed genus: (i) Methanothermobacter thermautotrophicus comb. nov., containing eight isolates, six of which are able to utilize formate (type strain deltaHT); (ii) Methanothermobacter wolfeii comb. nov., containing four formate-utilizing isolates (type strain DSM 2970T); (iii) Methanothermobacter marburgensis sp. nov., containing three obligately autotrophic isolates (type strain MarburgT). Of the nine isolates formerly referred to as Methanobacterium thermoformicicum, six were reclassified as Methanothermobacter thermautotrophicus and three as Methanothermobacter wolfeii.
[Mh] Termos MeSH primário: Methanobacteriaceae/classificação
Methanobacteriaceae/genética
Methanobacterium/classificação
Methanobacterium/genética
[Mh] Termos MeSH secundário: Antígenos Arqueais/classificação
Tipagem de Bacteriófagos
Sequência de Bases
DNA Arqueal/genética
DNA Ribossômico/genética
Methanobacteriaceae/imunologia
Methanobacteriaceae/fisiologia
Methanobacterium/imunologia
Methanobacterium/fisiologia
Dados de Sequência Molecular
Fenótipo
Filogenia
Plasmídeos
RNA Ribossômico 16S/genética
Alinhamento de Sequência
Análise de Sequência de DNA
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Antigens, Archaeal); 0 (DNA, Archaeal); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:0006
[Cu] Atualização por classe:061115
[Lr] Data última revisão:
061115
[Sb] Subgrupo de revista:IM; S
[Da] Data de entrada para processamento:000529
[St] Status:MEDLINE


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[PMID]:10591013
[Au] Autor:Kostyukova AS; Gongadze GM; Polosina YY; Bonch-Osmolovskaya EA; Miroshnichenko ML; Chernyh NA; Obraztsova MV; Svetlichny VA; Messner P; Sleytr UB; L'Haridon S; Jeanthon C; Prieur D
[Ad] Endereço:Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
[Ti] Título:Investigation of structure and antigenic capacities of Thermococcales cell envelopes and reclassification of "Caldococcus litoralis" Z-1301 as Thermococcus litoralis Z-1301.
[So] Source:Extremophiles;3(4):239-45, 1999 Nov.
[Is] ISSN:1431-0651
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Fourteen strains of hyperthermophilic organotrophic anaerobic marine Archaea were isolated from shallow water and deep-sea hot vents, and four of them were characterized. These isolates, eight previously published strains, and six type strains of species of the order Thermococcales were selected for the study of cell wall components by means of thin sectioning or freeze-etching electron microscopy. The cell envelopes of most isolates were shown to consist of regularly arrayed surface protein layers, either single or double, with hexagonal lattice (p6) symmetry, as the exclusive constituents outside the cytoplasmic membrane. The S-layers studied differed in center-to-center spacing and molecular mass of the constituent protein subunits. Polyclonal antisera raised against the cells of 10 species were found to be species-specific and allowed 12 new isolates from shallow water hot vents to be identified as representatives of the species Thermococcus litoralis, Thermococcus stetteri, Thermococcus chitonophagus, and Thermococcus pacificus. Of the 7 deep-sea isolates, only 1 was identified as a T. litoralis strain. Thus, hyperthermophilic marine organotrophic isolates obtained from deep-sea hot vents showed greater diversity with regard to their S-layer proteins than shallow water isolates.
[Mh] Termos MeSH primário: Antígenos Arqueais/análise
Thermococcales/classificação
Thermococcus/classificação
[Mh] Termos MeSH secundário: Membrana Celular/imunologia
Membrana Celular/ultraestrutura
Água do Mar/microbiologia
Thermococcales/imunologia
Thermococcales/ultraestrutura
Thermococcus/imunologia
Thermococcus/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, Archaeal)
[Em] Mês de entrada:9912
[Cu] Atualização por classe:061115
[Lr] Data última revisão:
061115
[Sb] Subgrupo de revista:IM; S
[Da] Data de entrada para processamento:991211
[St] Status:MEDLINE


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[PMID]:16348508
[Au] Autor:Visser FA; van Lier JB; Macario AJ; Conway de Macario E
[Ad] Endereço:Department of Microbiology and Department of Environmental Technology and Wadsworth Center for Laboratories and Research, New York State Department of Health, State University of New York at Albany, Albany, New York 12201-0509.
[Ti] Título:Diversity and population dynamics of methanogenic bacteria in a granular consortium.
[So] Source:Appl Environ Microbiol;57(6):1728-34, 1991 Jun.
[Is] ISSN:0099-2240
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Upflow anaerobic sludge blanket bioreactor granules were used as an experimental model microbial consortium to study the dynamics and distribution of methanogens. Immunologic methods revealed a considerable diversity of methanogens that was greater in mesophilic granules than in the same granules 4 months after a temperature shift from 38 to 55 degrees C. During this period, the sizes of the methanogenic subpopulations changed with distinctive profiles after the initial reduction caused by the shift. Methanogens antigenically related to Methanobrevibacter smithii PS and ALI, Methanobacterium hungatei JF1, and Methanosarcina thermophila TM1 increased rapidly, reached a short plateau, and then fell to lower concentrations that persisted for the duration of the experiment. A methanogen related to Methanogenium cariaci JR1 followed a similar profile at the beginning, but it soon diminished below detection levels. Methanothrix rods weakly related to the strain Opfikon increased rapidly, reaching a high-level, long-lasting plateau. Two methanogens related to Methanobrevibacter arboriphilus AZ and Methanobacterium thermoautotrophicum DeltaH emerged from very low levels before the temperature shift and multiplied to attain their highest numbers 4 months after the shift. Histochemistry and immunohistochemistry revealed thick layers, globular clusters, and lawns of variable density which were distinctive of the methanogens related to M. thermoautotrophicum DeltaH, M. thermophila TM1, and M. arboriphilus AZ and M. soehngenii Opfikon, respectively, in thin sections of granules grown at 55 degrees C for 4 months. Mesophilic granules showed a different pattern of methanogenic subpopulations.
[Mh] Termos MeSH primário: Biodiversidade
Reatores Biológicos/microbiologia
Euryarchaeota/fisiologia
Esgotos/microbiologia
[Mh] Termos MeSH secundário: Anticorpos Antiarchaea/metabolismo
Antígenos Arqueais/análise
Contagem de Colônia Microbiana
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Archaeal); 0 (Antigens, Archaeal); 0 (Sewage)
[Em] Mês de entrada:1006
[Cu] Atualização por classe:170219
[Lr] Data última revisão:
170219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:910601
[St] Status:MEDLINE


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[PMID]:16347990
[Au] Autor:Macario AJ; Conway de Macario E; Ney U; Schoberth SM; Sahm H
[Ad] Endereço:Wadsworth Center for Laboratories and Research, New York State Department of Health, and School of Public Health, State University of New York, Albany, New York, 12201-0509.
[Ti] Título:Shifts in methanogenic subpopulations measured with antibody probes in a fixed-bed loop anaerobic bioreactor treating sulfite evaporator condensate.
[So] Source:Appl Environ Microbiol;55(8):1996-2001, 1989 Aug.
[Is] ISSN:0099-2240
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A fixed-bed loop, high-rate anaerobic bioreactor treating sulfite evaporator condensate was sampled when it reached steady state and afterwards following perturbations during a 14-month period. By using immunotechnology, it was observed that shifts in methanogenic subpopulations occurred in association with perturbations, such as restarting and relocating the biomass into a different tank. Methanogens related to Methanobacterium bryantii MoHG and Methanobrevibacter smithii ALI were numerous throughout the observation period, while Methanosarcina mazei S6 and Methanosarcina thermophila TM1 were found in the early and late samples, respectively. Also, Methanobacterium formicicum was more numerous at the top portion of the bioreactor, while Methanobrevibacter arboriphilus AZ and DC were at the bottom. Sample formalinization required for prolonged storage proved suitable for antigen preservation.
[Mh] Termos MeSH primário: Biodiversidade
Reatores Biológicos/microbiologia
Euryarchaeota/fisiologia
[Mh] Termos MeSH secundário: Anticorpos Antiarchaea/metabolismo
Antígenos Arqueais/análise
Euryarchaeota/isolamento & purificação
Formaldeído/química
Técnicas Microbiológicas/métodos
Estresse Fisiológico/fisiologia
Sulfitos/química
Fatores de Tempo
Eliminação de Resíduos Líquidos/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Archaeal); 0 (Antigens, Archaeal); 0 (Sulfites); 1HG84L3525 (Formaldehyde)
[Em] Mês de entrada:1006
[Cu] Atualização por classe:170219
[Lr] Data última revisão:
170219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:890801
[St] Status:MEDLINE


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[PMID]:16347591
[Au] Autor:Fielding ER; Archer DB; Conway de Macario E; Macario AJ
[Ad] Endereço:Institute of Food Research, Agricultural and Food Research Council, Colney Lane, Norwich NR4 7UA, UK.
[Ti] Título:Isolation and characterization of methanogenic bacteria from landfills.
[So] Source:Appl Environ Microbiol;54(3):835-6, 1988 Mar.
[Is] ISSN:0099-2240
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Methanogenic bacteria were isolated from landfill sites in the United Kingdom. Strains of Methanobacterium formicicum, Methanosarcina barkeri, several different immunotypes of Methanobacterium bryantii, and a coccoid methanogen distinct from the reference immunotypes were identified.
[Mh] Termos MeSH primário: Microbiologia Ambiental
Euryarchaeota/isolamento & purificação
Euryarchaeota/fisiologia
Eliminação de Resíduos
[Mh] Termos MeSH secundário: Anticorpos Antiarchaea/metabolismo
Antígenos Arqueais/metabolismo
Técnicas de Tipagem Bacteriana
Biodiversidade
Euryarchaeota/classificação
Euryarchaeota/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Antibodies, Archaeal); 0 (Antigens, Archaeal)
[Em] Mês de entrada:1006
[Cu] Atualização por classe:100920
[Lr] Data última revisão:
100920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:880301
[St] Status:MEDLINE


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[PMID]:16347581
[Au] Autor:Kobayashi HA; Conway de Macario E; Williams RS; Macario AJ
[Ad] Endereço:BP America, Cleveland, Ohio 44128.
[Ti] Título:Direct characterization of methanogens in two high-rate anaerobic biological reactors.
[So] Source:Appl Environ Microbiol;54(3):693-8, 1988 Mar.
[Is] ISSN:0099-2240
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The methanogenic flora from two types of turbulent, high-rate reactors was studied by immunologic methods as well as by phase-contrast, fluorescence, and scanning electron microscopy. The reactors were a fluidized sand-bed biofilm ANITRON reactor and an ultrafiltration membrane-associated suspended growth MARS reactor (both trademarks of Air Products and Chemicals, Inc., Allentown, Pa.). Conventional microscopic methods revealed complex mixtures of microbes of a range of sizes and shapes, among which morphotypes resembling Methanothrix spp. and Methanosarcina spp. were noticed. Precise identification of these and other methanogens was accomplished by antigenic fingerprinting with a comprehensive panel of calibrated antibody probes of predefined specificity spectra. The methanogens identified showed morphotypes and antigenic fingerprints indicating their close similarity with the following reference organisms: Methanobacterium formicicum MF and Methanosarcina barkeri W in the ANITRON reactor only; Methanosarcina barkeri R1M3, M. mazei S6, Methanogenium cariaci JR1, and Methanobrevibacter arboriphilus AZ in the MARS reactor only; and Methanobrevibacter smithii ALI and Methanothrix soehngenii Opfikon in both reactors. Species diversity and distribution appeared to be, at least in part, dependent on the degree of turbulence inside the reactor.
[Mh] Termos MeSH primário: Reatores Biológicos/microbiologia
Euryarchaeota/fisiologia
[Mh] Termos MeSH secundário: Anaerobiose/fisiologia
Anticorpos Antiarchaea/metabolismo
Antígenos Arqueais/metabolismo
Técnicas de Tipagem Bacteriana
Biodiversidade
Euryarchaeota/classificação
Euryarchaeota/imunologia
Euryarchaeota/isolamento & purificação
Microscopia Eletrônica de Varredura
Eliminação de Resíduos/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Archaeal); 0 (Antigens, Archaeal)
[Em] Mês de entrada:1006
[Cu] Atualização por classe:170219
[Lr] Data última revisão:
170219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:880301
[St] Status:MEDLINE


  10 / 10 MEDLINE  
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[PMID]:16347541
[Au] Autor:Macario AJ; Conway de Macario E
[Ad] Endereço:Wadsworth Center for Laboratories and Research, New York State Department of Health, and School of Public Health, State University of New York, Albany, New York 12201.
[Ti] Título:Quantitative immunologic analysis of the methanogenic flora of digestors reveals a considerable diversity.
[So] Source:Appl Environ Microbiol;54(1):79-86, 1988 Jan.
[Is] ISSN:0099-2240
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To determine which methanogens occur in digestors, we performed a quantitative immunologic analysis of a variety of samples. A comprehensive panel of calibrated polyclonal antibody probes of predefined specificity spectra was used. This allowed precise identification of bacteria by antigenic fingerprinting. A considerable diversity of methanogens was uncovered, much larger than previously reported, encompassing at least 14 strains of 11 species. Strategies were developed to measure the load of any given methanogen in a sample and to compare samples quantitatively. Two methanogens were found to predominate which were antigenically closely related with either Methanobacterium formicicum MF or Methanobrevibacter arboriphilus AZ. Fundamental data, probes, and methods are now available to monitor methanogenic subpopulations during digestor operation and thus learn about their respective roles and predictive significance.
[Mh] Termos MeSH primário: Anticorpos Antiarchaea/metabolismo
Biodiversidade
Reatores Biológicos/microbiologia
Euryarchaeota/classificação
Euryarchaeota/imunologia
Eliminação de Resíduos
[Mh] Termos MeSH secundário: Antígenos Arqueais/análise
Euryarchaeota/isolamento & purificação
Methanobacterium/isolamento & purificação
Methanobacterium/fisiologia
Methanobrevibacter/isolamento & purificação
Methanobrevibacter/fisiologia
Eliminação de Resíduos/métodos
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Antibodies, Archaeal); 0 (Antigens, Archaeal)
[Em] Mês de entrada:1006
[Cu] Atualização por classe:170219
[Lr] Data última revisão:
170219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:880101
[St] Status:MEDLINE



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