Base de dados : MEDLINE
Pesquisa : D23.050.161.845 [Categoria DeCS]
Referências encontradas : 4170 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 417 ir para página                         

  1 / 4170 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29281719
[Au] Autor:Druszczynska M; Wlodarczyk M; Kielnierowski G; Seweryn M; Wawrocki S; Rudnicka W
[Ad] Endereço:Division of Cell Immunology, Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
[Ti] Título:CD14-159C/T polymorphism in the development of delayed skin hypersensitivity to tuberculin.
[So] Source:PLoS One;12(12):e0190106, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The skin tuberculin test (TST), an example of a delayed-type hypersensitivity (DTH) reaction, is based on measuring the extent of skin induration to mycobacterial tuberculin (PPD). Little is known about the genetic basis of TST reactivity, widely used for diagnosing TB infection. The study investigated the relationship of the single base change polymorphic variants in CD14 gene (CD14(-159C/T)) with the development of DTH to PPD in BCG-vaccinated Polish Caucasian individuals. We found persistent lack of TST reactivity in about 40% of healthy subjects despite receiving more than one dose of BCG. The TST size was negatively correlated with the number of BCG inoculations. The distribution of C/T genotype was significantly more frequent among TST-negative compared with TST-positive individuals. The concentration of serum sCD14 was positively associated with mCD14 expression, but not with the TST status or CD14(-159C/T) polymorphism. A significant increase in mCD14 expression and serum sCD14 levels was found in TB group. We hypothesize that CD14(-159C/T) polymorphic variants might be one of genetic components in the response to attenuated M. bovis BCG bacilli.
[Mh] Termos MeSH primário: Hipersensibilidade Tardia
Receptores de Lipopolissacarídeos/imunologia
Polimorfismo Genético
Tuberculina/efeitos adversos
[Mh] Termos MeSH secundário: Adulto
Feminino
Seres Humanos
Masculino
Teste Tuberculínico
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Lipopolysaccharide Receptors); 0 (Tuberculin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171228
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190106


  2 / 4170 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28372590
[Au] Autor:Roperto S; Varano M; Russo V; Lucà R; Cagiola M; Gaspari M; Ceccarelli DM; Cuda G; Roperto F
[Ad] Endereço:Dipartimento di Medicina Veterinaria e delle Produzioni Animali, Università di Napoli Federico II, Naples, Italy. sante.roperto@unina.it.
[Ti] Título:Proteomic analysis of protein purified derivative of Mycobacterium bovis.
[So] Source:J Transl Med;15(1):68, 2017 Apr 03.
[Is] ISSN:1479-5876
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Tuberculin skin test based on in vivo intradermal inoculation of purified protein derivative from Mycobacterium bovis (bPPD) is the diagnostic test for the control and surveillance of bovine tuberculosis (bTB). METHODS: Proteomic analysis was performed on different bPPD preparations from M. bovis, strain AN5. Proteins were precipitated from bPPD solutions by TCA precipitation. The proteome of bPPD preparations was investigated by bottom-up proteomics, which consisted in protein digestion and nano-LC-MS/MS analysis. Mass spectrometry analysis was performed on a Q-exactive hybrid quadrupole-Orbitrap mass spectrometer coupled online to an Easy nano-LC1000 system. RESULTS: Three hundred and fifty-six proteins were identified and quantified by at least 2 peptides (99% confidence per peptide). One hundred and ninety-eight proteins, which had not been previously described, were detected; furthermore, the proteomic profile shared 80 proteins with previous proteomes from bPPDs from the United Kingdom and Brazil and 139 protein components from bPPD from Korea. Locus name of M. bovis (Mb) with orthologs from M. tuberculosis H37Rv, comparative gene and protein length, molecular mass, functional categories, gene name and function of each protein were reported. Ninety-two T cell mycobacterial antigens responsible for delayed-type hypersensitivity were detected, fifty-two of which were not previously reported in any bPPD proteome. Data are available via ProteomeXchange with identifier PXD005920. CONCLUSIONS: This study represents the highest proteome coverage of bPPD preparations to date. Since proteins perform cellular functions essential to health and/or disease, obtaining knowledge of their presence and variance is of great importance in understanding disease states and for advancing translational studies. Therefore, to better understand Mycobacterium tuberculosis complex biology during infection, survival, and persistence, the reproducible evaluation of the proteins that catalyze and control these processes is critically important. More active and more specific tuberculins would be desirable. Indeed, many antigens contained within bPPD are currently responsible for the cross-reactivity resulting in false-positive results as they are shared between non-tuberculous and tuberculous mycobacteria.
[Mh] Termos MeSH primário: Mycobacterium bovis/metabolismo
Proteômica/métodos
Tuberculina/análise
[Mh] Termos MeSH secundário: Antígenos de Bactérias/metabolismo
Proteínas de Bactérias/isolamento & purificação
Cromatografia Líquida
Nanotecnologia
Coloração e Rotulagem
Linfócitos T/metabolismo
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Bacterial); 0 (Bacterial Proteins); 0 (Tuberculin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170405
[St] Status:MEDLINE
[do] DOI:10.1186/s12967-017-1172-1


  3 / 4170 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28338490
[Au] Autor:Muffly MK; Chen MI; Claure RE; Drover DR; Efron B; Fitch WL; Hammer GB
[Ad] Endereço:From the *Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University Medical Center, Stanford, California; †Department of Statistics, Stanford University, Stanford, California; and ‡Division of Biomaterials and Advanced Drug Delivery, Stanford University Medical Center, Stanford, California.
[Ti] Título:Small-Volume Injections: Evaluation of Volume Administration Deviation From Intended Injection Volumes.
[So] Source:Anesth Analg;125(4):1192-1199, 2017 Oct.
[Is] ISSN:1526-7598
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In the perioperative period, anesthesiologists and postanesthesia care unit (PACU) nurses routinely prepare and administer small-volume IV injections, yet the accuracy of delivered medication volumes in this setting has not been described. In this ex vivo study, we sought to characterize the degree to which small-volume injections (≤0.5 mL) deviated from the intended injection volumes among a group of pediatric anesthesiologists and pediatric postanesthesia care unit (PACU) nurses. We hypothesized that as the intended injection volumes decreased, the deviation from those intended injection volumes would increase. METHODS: Ten attending pediatric anesthesiologists and 10 pediatric PACU nurses each performed a series of 10 injections into a simulated patient IV setup. Practitioners used separate 1-mL tuberculin syringes with removable 18-gauge needles (Becton-Dickinson & Company, Franklin Lakes, NJ) to aspirate 5 different volumes (0.025, 0.05, 0.1, 0.25, and 0.5 mL) of 0.25 mM Lucifer Yellow (LY) fluorescent dye constituted in saline (Sigma Aldrich, St. Louis, MO) from a rubber-stoppered vial. Each participant then injected the specified volume of LY fluorescent dye via a 3-way stopcock into IV tubing with free-flowing 0.9% sodium chloride (10 mL/min). The injected volume of LY fluorescent dye and 0.9% sodium chloride then drained into a collection vial for laboratory analysis. Microplate fluorescence wavelength detection (Infinite M1000; Tecan, Mannedorf, Switzerland) was used to measure the fluorescence of the collected fluid. Administered injection volumes were calculated based on the fluorescence of the collected fluid using a calibration curve of known LY volumes and associated fluorescence.To determine whether deviation of the administered volumes from the intended injection volumes increased at lower injection volumes, we compared the proportional injection volume error (loge [administered volume/intended volume]) for each of the 5 injection volumes using a linear regression model. Analysis of variance was used to determine whether the absolute log proportional error differed by the intended injection volume. Interindividual and intraindividual deviation from the intended injection volume was also characterized. RESULTS: As the intended injection volumes decreased, the absolute log proportional injection volume error increased (analysis of variance, P < .0018). The exploratory analysis revealed no significant difference in the standard deviations of the log proportional errors for injection volumes between physicians and pediatric PACU nurses; however, the difference in absolute bias was significantly higher for nurses with a 2-sided significance of P = .03. CONCLUSIONS: Clinically significant dose variation occurs when injecting volumes ≤0.5 mL. Administering small volumes of medications may result in unintended medication administration errors.
[Mh] Termos MeSH primário: Anestesiologistas/normas
Composição de Medicamentos/métodos
Composição de Medicamentos/normas
Enfermeiras e Enfermeiros/normas
Preparações Farmacêuticas/normas
Seringas/normas
[Mh] Termos MeSH secundário: Calibragem/normas
Seres Humanos
Injeções
Preparações Farmacêuticas/química
Tuberculina/administração & dosagem
Tuberculina/química
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pharmaceutical Preparations); 0 (Tuberculin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE
[do] DOI:10.1213/ANE.0000000000001976


  4 / 4170 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28222109
[Au] Autor:Marín D; Marín N; Del Corral H; López L; Ramirez-Agudelo ME; Rojas CA; Arbeláez MP; García LF; Rojas M
[Ad] Endereço:Grupo de Epidemiología, Facultad Nacional de Salud Pública, Universidad de Antioquia, Medellín, Colombia.
[Ti] Título:PPD-induced monocyte mitochondrial damage is associated with a protective effect to develop tuberculosis in BCG vaccinated individuals: A cohort study.
[So] Source:PLoS One;12(2):e0171930, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: The mechanisms of mononuclear phagocyte death have been associated with the permissiveness and resistance to mycobacterial replication, but it remains unknown whether or not they help predict the risk of developing TB. OBJECTIVE: To describe the factors associated with the induction of monocyte mitochondrial and membrane damage in response to PPD as well as determine if this type of damage might predict the susceptibility of developing active tuberculosis in a cohort of household contacts (HHCs) from Medellin, Colombia from 2005 to 2008. METHODS: The prospective cohort study contains 2060 HHCs patients with pulmonary tuberculosis who were meticulously followed for two years. A survey of the socio-demographic, clinical, epidemiological factors and blood samples were collected. Mononuclear cell cultures were stimulated with or without PPD and the type of monocyte death was determined by the flow of cytometry, an indicator was also used for its analysis. Logistic regression was adjusted by the Generalized Estimations Equations and the survival was estimated with the Kaplan-Meier and Cox regression. Confidence intervals were used for estimating the association. RESULTS: 1,859 out of 2,060 blood samples of the HHCs patients analyzed showed monocyte death. In response to PPD, 83.4% underwent mitochondrial damage while 50.9% had membrane damage. The membrane damage in response to PPD was higher in children under 4 years (OR: 1.57; (95% CI: 1.1 to 2.4) and the HHCs who slept regularly in the same household has an index case of (OR: 1.54; 95% CI: 1.0 to 2.3). After adjustment by age, comorbidities, nutritional status, proximity to index case and overcrowding, the risk of developing active TB among BCG vaccinated HHCs individuals with induction of mitochondrial damage was HR = 0.19 (95% CI: 0.1 to 0.5). CONCLUSIONS: The induction of monocytes mitochondrial damage by PPD stimulation correlates with protection of TB disease development in BCG-vaccinated HHCs. This represents a potential tool to predict susceptibility of developing active disease in this population.
[Mh] Termos MeSH primário: Vacina BCG
Mitocôndrias/efeitos dos fármacos
Monócitos/efeitos dos fármacos
Tuberculina/toxicidade
Tuberculose/prevenção & controle
[Mh] Termos MeSH secundário: Adolescente
Adulto
Morte Celular
Membrana Celular/efeitos dos fármacos
Membrana Celular/ultraestrutura
Células Cultivadas
Criança
Pré-Escolar
Colômbia/epidemiologia
Busca de Comunicante
Suscetibilidade a Doenças
Características da Família
Feminino
Seguimentos
Seres Humanos
Lactente
Masculino
Meia-Idade
Mitocôndrias/ultraestrutura
Monócitos/ultraestrutura
Estudos Prospectivos
Fatores Socioeconômicos
Tuberculose/epidemiologia
Tuberculose/imunologia
Vacinação
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (BCG Vaccine); 0 (Tuberculin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170222
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0171930


  5 / 4170 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28168727
[Au] Autor:Moafi M; Rezvan H; Sherkat R; Taleban R; Asilian A; Hamid Zarkesh-Esfahani S; Nilforoushzadeh MA; Jaffary F; Mansourian M; Sokhanvari F; Ansari N
[Ad] Endereço:Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran.
[Ti] Título:Comparison of pro-inflammatory cytokines of non-healing and healing cutaneous leishmaniasis.
[So] Source:Scand J Immunol;85(4):291-299, 2017 Apr.
[Is] ISSN:1365-3083
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cutaneous leishmaniasis (CL) heals spontaneously within several weeks or months, but, in rare cases, CL-active lesions last for many years. In this study, we assessed cell-mediated immunity in non-healing CL through the measurement of three pro-inflammatory cytokines: Interferon-γ (IFN-γ), IL-17a and CXCL-11. For this, 32 patients afflicted with healing or non-healing CL were recruited in this study. Peripheral blood mononuclear cells (PBMCs) of every patient were treated with three antigens: purified protein derivative (PPD), soluble Leishmania antigen (SLA) and phytohaemagglutinin (PHA). Cytokine quantification was performed using enzyme-linked immunosorbent assay (ELISA) method. Results of our study showed that neither cytokine produced in the presence of a PPD stimulator (as an irrelevant antigen) significantly differed between the healing and non-healing groups (P-value ≥0.05 for all of them). However, IFN-γ, CXCL-11 and IL-17a levels produced in the presence of PHA or SLA were significantly higher within the healing than in the non-healing group (P-value <0.01 for all of them). It seems that appropriate levels of IFN-γ, as well as IL-17a and CXCL-11, contribute to the control of Leishmania infection.
[Mh] Termos MeSH primário: Quimiocina CXCL11/sangue
Interferon gama/sangue
Interleucina-17/sangue
Leishmania/imunologia
Leishmaniose Cutânea/sangue
Leishmaniose Cutânea/imunologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Antígenos de Protozoários/imunologia
Criança
Estudos Transversais
Ensaio de Imunoadsorção Enzimática
Feminino
Seres Humanos
Leishmaniose Cutânea/parasitologia
Masculino
Meia-Idade
Fito-Hemaglutininas/imunologia
Tuberculina/imunologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Protozoan); 0 (CXCL11 protein, human); 0 (Chemokine CXCL11); 0 (IL17A protein, human); 0 (Interleukin-17); 0 (Phytohemagglutinins); 0 (Tuberculin); 82115-62-6 (Interferon-gamma)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170421
[Lr] Data última revisão:
170421
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170208
[St] Status:MEDLINE
[do] DOI:10.1111/sji.12534


  6 / 4170 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28087302
[Au] Autor:Mancuso JD; Mody RM; Olsen CH; Harrison LH; Santosham M; Aronson NE
[Ad] Endereço:Department of Preventive Medicine and Biostatistics, Uniformed Services University of the Health Sciences, Bethesda, MD. Electronic address: james.mancuso@usamru-k.org.
[Ti] Título:The Long-term Effect of Bacille Calmette-Guérin Vaccination on Tuberculin Skin Testing: A 55-Year Follow-Up Study.
[So] Source:Chest;152(2):282-294, 2017 Aug.
[Is] ISSN:1931-3543
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Bacille Calmette-Guérin (BCG) vaccination is known to cause false-positive tuberculin skin test (TST) results from cross-reactions with mycobacterial antigens. However, the duration of BCG vaccination influence on the TST is poorly characterized. The objective of this study was to assess the long-term effect of BCG vaccination on TST reactivity. METHODS: Data on TST reactivity were prospectively collected during 1935 to 1947 as part of a clinical trial among Native Americans/Alaskan Natives and were retrospectively collected thereafter between 1948 and 1998. TST induration of ≥ 10 mm was defined as a positive reaction. Kaplan-Meier analysis and multivariate Cox regression were used to compare the time to TST conversion and reversion between the BCG and placebo groups. RESULTS: BCG vaccination after infancy was associated with an increased risk of TST reactivity in the first 15 years after vaccination (adjusted hazard ratio [HR], 2.33). This association remained during the interval 16 to 55 years after vaccination, although the effect was attenuated (adjusted HR, 1.26). Age at vaccination modestly impacted the effect of BCG on TST results in the first 15 years. Positive TST results among the BCG-vaccinated group were more likely to revert to negative results during the first 15 years but not in the latter period. CONCLUSIONS: This study provides evidence that BCG vaccination after infancy may influence TST results beyond the 10-year period conventionally accepted by the Centers for Disease Control and Prevention (CDC), extending up to 55 years after vaccination. This suggests that BCG vaccination should be taken into account when interpreting TST results regardless of the time elapsed since vaccination.
[Mh] Termos MeSH primário: Vacina BCG/imunologia
Tuberculose/epidemiologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Criança
Pré-Escolar
Feminino
Seguimentos
Seres Humanos
Incidência
Lactente
Recém-Nascido
Estimativa de Kaplan-Meier
Masculino
Meia-Idade
Estudos Prospectivos
Estudos Retrospectivos
Tuberculina/imunologia
Teste Tuberculínico
Tuberculose/imunologia
Estados Unidos/epidemiologia
Vacinação
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (BCG Vaccine); 0 (Tuberculin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170115
[St] Status:MEDLINE


  7 / 4170 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28061954
[Au] Autor:Wu J; Wang S; Lu C; Shao L; Gao Y; Zhou Z; Huang H; Zhang Y; Zhang W
[Ad] Endereço:Institute of Infectious Diseases, Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai 200040, China.
[Ti] Título:Multiple cytokine responses in discriminating between active tuberculosis and latent tuberculosis infection.
[So] Source:Tuberculosis (Edinb);102:68-75, 2017 Jan.
[Is] ISSN:1873-281X
[Cp] País de publicação:Scotland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Cytokines play an important role in cell-mediated immune responses against Mycobacterium tuberculosis (Mtb) infection. Cytokine profile specifically associated with active tuberculosis (ATB) patients, subjects with latent tuberculosis infection (LTBI) and non-infected individuals remains to be determined. METHODS: We enrolled a total of 92 subjects including patients with ATB (n = 25), LTBI (n = 36) and healthy controls (HC, n = 31) to investigate the cytokine production by peripheral blood mononuclear cells after Mtb purified protein derivative (PPD) stimulation which was evaluated by a beads-based multiplex assay system. RESULTS: The production of IL-1ß, IL-2, IL-6, IL-10, IL-17, G-CSF, IFN-γ, IP-10, MIP-1α and TNF-α was abundantly induced by PPD in all three groups. The levels of IL-2, IL-10, IFN-γ, IP-10 and TNF-α were significantly higher in LTBI group than in ATB group. The combination of PPD-stimulated IL-2 and IL-10 accurately identified 84.0% of ATB and 88.9% of LTBI. We validated the use of PPD-stimulated IL-2 and IL-10 test combined with T-SPOT.TB test in a cohort of 44 subjects with TB suspicion. The sensitivity and specificity of the combined test were 83.3% and 92.3%, respectively. The PPD-stimulated IL-2/IFN-γ ratio (p < 0.001) in LTBI subjects was significantly higher than in active TB patients. CONCLUSION: Our study identified cytokine patterns characteristic of ATB and LTBI. Cytokines such as IL-2 and IL-10 may serve as biomarkers for distinguishing ATB from LTBI and healthy control and may contribute to intervention and improvement in TB diagnosis.
[Mh] Termos MeSH primário: Citocinas/biossíntese
Tuberculose/diagnóstico
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Biomarcadores/metabolismo
Estudos de Casos e Controles
Células Cultivadas
Criança
Pré-Escolar
Diagnóstico Diferencial
Feminino
Seres Humanos
Testes de Liberação de Interferon-gama
Tuberculose Latente/diagnóstico
Tuberculose Latente/imunologia
Leucócitos Mononucleares/imunologia
Masculino
Meia-Idade
Estudos Prospectivos
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Tuberculina/imunologia
Teste Tuberculínico
Tuberculose/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Cytokines); 0 (Tuberculin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170108
[St] Status:MEDLINE


  8 / 4170 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28061860
[Au] Autor:Nuru A; Mamo G; Zewude A; Mulat Y; Yitayew G; Admasu A; Medhin G; Pieper R; Ameni G
[Ad] Endereço:Aklilu Lemma Institute of Pathobiology, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia. hamduanwar@yahoo.com.
[Ti] Título:Preliminary investigation of the transmission of tuberculosis between farmers and their cattle in smallholder farms in northwestern Ethiopia: a cross-sectional study.
[So] Source:BMC Res Notes;10(1):31, 2017 Jan 07.
[Is] ISSN:1756-0500
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The feeding habits and close physical contact between Ethiopian farmers and their cattle promote the transmission of tuberculosis (TB) between the farmers and their cattle. This study aimed to investigate the transmission of TB between farmers and their cattle in smallholder farms in northwestern Ethiopia. RESULTS: A total of 70 human TB lymphadenitis (TBLN) cases visiting the Felegehiwot Comprehensive Specialized Hospital in Bahir Dar City and 660 cattle were investigated. Half of the cattle were owned by households with TB cases, and the remaining half by TB free households. Among the 70 human TBLN patients interviewed, 65.7% (46 out of 70) of the respondents were not aware of zoonotic TB, and 67.1% (47/70) of them consumed raw milk. Positive cultures of TB were obtained in 40 of the 70 cases where TBLN tests were positive with fine needle aspiration cytology. Spoligotyping resulted in 31 different patterns, of which 25 isolates were Mycobacterium (M.) tuberculosis, and the remaining were M. africanum (4 isolates) and M. bovis (2 isolates). None of the animals showed positive test results for bovine TB by comparative intradermal tuberculin test. CONCLUSIONS: Based on the identification of M. bovis from two patients diagnosed with TBLN, we obtained preliminary evidence of zoonotic transmission of TB in northwestern Ethiopia. We did not identify a direct route of transmission between cattle and its owners. This is the objective of further investigations.
[Mh] Termos MeSH primário: Fazendeiros
Tuberculose Bovina/transmissão
Tuberculose dos Linfonodos/transmissão
Tuberculose/transmissão
Zoonoses/transmissão
[Mh] Termos MeSH secundário: Animais
Biópsia por Agulha Fina
Bovinos
Estudos Transversais
Etiópia
Contaminação de Alimentos
Microbiologia de Alimentos
Seres Humanos
Mycobacterium bovis
Exposição Ocupacional
Tuberculina/química
Tuberculose Bovina/diagnóstico
Tuberculose Bovina/microbiologia
Tuberculose dos Linfonodos/diagnóstico
Tuberculose dos Linfonodos/microbiologia
Zoonoses/diagnóstico
Zoonoses/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Tuberculin)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170108
[St] Status:MEDLINE
[do] DOI:10.1186/s13104-016-2349-z


  9 / 4170 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27577087
[Au] Autor:Chukwuanukwu RC; Onyenekwe CC; Martinez-Pomares L; Flynn R; Singh S; Amilo GI; Agbakoba NR; Okoye JO
[Ad] Endereço:Medical Laboratory Science Department, Nnamdi Azikiwe University, Nnewi Campus, Nigeria.
[Ti] Título:Modulation of the immune response to Mycobacterium tuberculosis during malaria/M. tuberculosis co-infection.
[So] Source:Clin Exp Immunol;187(2):259-268, 2017 Feb.
[Is] ISSN:1365-2249
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Tuberculosis (TB) causes significant morbidity and mortality on a global scale. The African region has 24% of the world's TB cases. TB overlaps with other infectious diseases such as malaria and HIV, which are also highly prevalent in the African region. TB is a leading cause of death among HIV-positive patients and co-infection with HIV and TB has been described as a syndemic. In view of the overlapping epidemiology of these diseases, it is important to understand the dynamics of the immune response to TB in the context of co-infection. We investigated the cytokine response to purified protein derivative (PPD) in peripheral blood mononuclear cells from TB patients co-infected with HIV or malaria and compared it to that of malaria- and HIV-free TB patients. A total of 231 subjects were recruited for this study and classified into six groups; untreated TB-positive, TB positive subjects on TB drugs, TB- and HIV-positive, TB- and malaria-positive, latent TB and apparently healthy control subjects. Our results demonstrate maintenance of interferon (IFN)-γ production in HIV and malaria co-infected TB patients in spite of lower CD4 counts in the HIV-infected cohort. Malaria co-infection caused an increase in the production of the T helper type 2 (Th2)-associated cytokine interleukin (IL)-4 and the anti-inflammatory cytokine IL-10 in PPD-stimulated cultures. These results suggest that malaria co-infection diverts immune response against M. tuberculosis towards a Th-2/anti-inflammatory response which might have important consequences for disease progression.
[Mh] Termos MeSH primário: Linfócitos T CD4-Positivos/imunologia
Infecções por HIV/imunologia
Malária/imunologia
Mycobacterium tuberculosis/imunologia
Tuberculose/imunologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Linfócitos T CD4-Positivos/microbiologia
Linfócitos T CD4-Positivos/parasitologia
Células Cultivadas
Criança
Coinfecção
Citocinas/metabolismo
Feminino
Infecções por HIV/complicações
Seres Humanos
Malária/complicações
Masculino
Meia-Idade
Nigéria
Equilíbrio Th1-Th2
Tuberculina/imunologia
Tuberculose/complicações
Adulto Jovem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (Tuberculin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170509
[Lr] Data última revisão:
170509
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160901
[St] Status:MEDLINE
[do] DOI:10.1111/cei.12861


  10 / 4170 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27863555
[Au] Autor:Roos EO; Buss P; de Klerk-Lorist LM; Hewlett J; Hausler GA; Rossouw L; McCall AJ; Cooper D; van Helden PD; Parsons SDC; Miller MA
[Ad] Endereço:DST/NRF Centre of Excellence for Biomedical TB Research, SAMRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town 8000, South Africa.
[Ti] Título:Test performance of three serological assays for the detection of Mycobacterium bovis infection in common warthogs (Phacochoerus africanus).
[So] Source:Vet Immunol Immunopathol;182:79-84, 2016 Dec.
[Is] ISSN:1873-2534
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Sporadic cases of bovine tuberculosis (bTB) have been reported in warthogs in Southern Africa and confirmed through mycobacterial culture. However, there are no validated ante-mortem tests currently available for bTB in warthogs. In this study, we evaluated the use of three serological assays for the detection of Mycobacterium bovis infection in warthogs; an indirect enzyme-linked immunosorbent assay (ELISA) using bovine purified protein derivative (PPD ) as a capture antigen (indirect PPD ELISA), as well as two commercial assays, the TB ELISA-VK and DPP VetTB Assay. Test performance of these assays was compared using sera from 35 warthogs of known Mycobacterium bovis infection status. All three assays were able to distinguish M. bovis-infected from uninfected individuals with high sensitivity (Se) and specificity (Sp) (indirect PPD ELISA Se: 88%, Sp: 89%; TB ELISA-VK 88%, 79%; DPP VetTB Assay 75%, 89%, respectively). The assays performed very similarly and the ELISA assays showed the greatest agreement (κ=0.89). These results indicate that M. bovis-infected warthogs develop measurable pathogen-specific humoral responses which can be used to distinguish them from uninfected animals. Therefore, serological assays have value as ante-mortem bTB diagnostic tests in warthogs.
[Mh] Termos MeSH primário: Mycobacterium bovis
Testes Sorológicos/veterinária
Doenças dos Suínos/diagnóstico
Suínos
Tuberculose/veterinária
[Mh] Termos MeSH secundário: Animais
Anticorpos Antibacterianos/sangue
Especificidade de Anticorpos
Ensaio de Imunoadsorção Enzimática/métodos
Ensaio de Imunoadsorção Enzimática/veterinária
Mycobacterium bovis/imunologia
Testes Sorológicos/métodos
África do Sul
Doenças dos Suínos/imunologia
Tuberculina/imunologia
Tuberculose/diagnóstico
Tuberculose/imunologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial); 0 (Tuberculin)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161120
[St] Status:MEDLINE



página 1 de 417 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde