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  1 / 121 MEDLINE  
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[PMID]:27546806
[Au] Autor:Grys TE; Kaushal S; Chowdhury Y; Dasari S; Mitchell NM; Magee DM; Blair JE; Colby TV; Lake DF
[Ad] Endereço:Department of Laboratory Medicine and Pathology, Mayo Clinic , Phoenix, Arizona 85054, United States.
[Ti] Título:Total and Lectin-Binding Proteome of Spherulin from Coccidioides posadasii.
[So] Source:J Proteome Res;15(10):3463-3472, 2016 Oct 07.
[Is] ISSN:1535-3907
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Coccidioides is a virulent dimorphic fungus that causes coccidioidomycosis (valley fever) in mammals, including humans. Although the genome has been sequenced, a proteomic analysis does not exist. To address this gap in proteomic knowledge, we generated the proteome of spherulin (a well-studied lysate of fungal spherules) and identified 1390 proteins. Some of the proteins included glycosylation enzymes, which led us to hypothesize that fungal glycosylation patterns may be different from those of mammals and could be exploited to detect Coccidioides in tissues. We performed lectin-based immunohistochemistry on formalin-fixed paraffin-embedded human patients' lung tissues. GSL-II (Griffonia simplificonia lectin II) and sWGA (succinylated wheat germ agglutinin) lectins bound specifically to endospores and spherules in infected lungs. To identify lectin-binding glycoproteins in spherulin, we performed lectin-affinity chromatography, followed by LC-MS/MS. A total of 195 glycoproteins from spherulin bound to GSL-II, 224 glycoproteins bound to sWGA, and 145 glycoproteins bound to both lectins. This is the first report of the specific reactivity of GSL-II and sWGA lectins to Coccidioides endospores and spherules in infected human tissues and the first listing of the Coccidioidal proteome from spherulin using sequences present in three Coccidioides databases: RefSeq, SwissProt, and The Broad Institute's Coccidioides Genome project.
[Mh] Termos MeSH primário: Coccidioides/química
Coccidioidina/química
Proteínas Fúngicas/análise
Lectinas/metabolismo
Proteoma/metabolismo
[Mh] Termos MeSH secundário: Cromatografia de Afinidade
Coccidioidomicose/diagnóstico
Coccidioidomicose/patologia
Glicoproteínas/análise
Glicoproteínas/metabolismo
Glicosilação
Seres Humanos
Imuno-Histoquímica
Pulmão/patologia
Ligação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Glycoproteins); 0 (Lectins); 0 (Proteome); 0 (spherulin); 12622-73-0 (Coccidioidin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160823
[St] Status:MEDLINE


  2 / 121 MEDLINE  
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[PMID]:26536128
[Au] Autor:Germer J; Mann K; Wörheide G; Jackson DJ
[Ad] Endereço:Department of Geobiology, Georg-August University of Göttingen, Göttingen, Germany.
[Ti] Título:The Skeleton Forming Proteome of an Early Branching Metazoan: A Molecular Survey of the Biomineralization Components Employed by the Coralline Sponge Vaceletia Sp.
[So] Source:PLoS One;10(11):e0140100, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The ability to construct a mineralized skeleton was a major innovation for the Metazoa during their evolution in the late Precambrian/early Cambrian. Porifera (sponges) hold an informative position for efforts aimed at unraveling the origins of this ability because they are widely regarded to be the earliest branching metazoans, and are among the first multi-cellular animals to display the ability to biomineralize in the fossil record. Very few biomineralization associated proteins have been identified in sponges so far, with no transcriptome or proteome scale surveys yet available. In order to understand what genetic repertoire may have been present in the last common ancestor of the Metazoa (LCAM), and that may have contributed to the evolution of the ability to biocalcify, we have studied the skeletal proteome of the coralline demosponge Vaceletia sp. and compare this to other metazoan biomineralizing proteomes. We bring some spatial resolution to this analysis by dividing Vaceletia's aragonitic calcium carbonate skeleton into "head" and "stalk" regions. With our approach we were able to identify 40 proteins from both the head and stalk regions, with many of these sharing some similarity to previously identified gene products from other organisms. Among these proteins are known biomineralization compounds, such as carbonic anhydrase, spherulin, extracellular matrix proteins and very acidic proteins. This report provides the first proteome scale analysis of a calcified poriferan skeletal proteome, and its composition clearly demonstrates that the LCAM contributed several key enzymes and matrix proteins to its descendants that supported the metazoan ability to biocalcify. However, lineage specific evolution is also likely to have contributed significantly to the ability of disparate metazoan lineages to biocalcify.
[Mh] Termos MeSH primário: Poríferos/metabolismo
Proteoma/metabolismo
[Mh] Termos MeSH secundário: Animais
Evolução Biológica
Calcificação Fisiológica/fisiologia
Carbonato de Cálcio/metabolismo
Anidrases Carbônicas/metabolismo
Cromatografia Líquida de Alta Pressão
Coccidioidina/classificação
Coccidioidina/metabolismo
Proteínas da Matriz Extracelular/metabolismo
Espectrometria de Massas
Filogenia
Poríferos/genética
Proteoma/análise
Esqueleto/metabolismo
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Extracellular Matrix Proteins); 0 (Proteome); 0 (spherulin); 12622-73-0 (Coccidioidin); EC 4.2.1.1 (Carbonic Anhydrases); H0G9379FGK (Calcium Carbonate)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151105
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0140100


  3 / 121 MEDLINE  
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[PMID]:26342082
[Au] Autor:Bamford NC; Snarr BD; Gravelat FN; Little DJ; Lee MJ; Zacharias CA; Chabot JC; Geller AM; Baptista SD; Baker P; Robinson H; Howell PL; Sheppard DC
[Ad] Endereço:From the Program in Molecular Structure and Function, Research Institute, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada, the Department of Biochemistry, University of Toronto, Toronto, Ontario M5S 1A8, Canada.
[Ti] Título:Sph3 Is a Glycoside Hydrolase Required for the Biosynthesis of Galactosaminogalactan in Aspergillus fumigatus.
[So] Source:J Biol Chem;290(46):27438-50, 2015 Nov 13.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Aspergillus fumigatus is the most virulent species within the Aspergillus genus and causes invasive infections with high mortality rates. The exopolysaccharide galactosaminogalactan (GAG) contributes to the virulence of A. fumigatus. A co-regulated five-gene cluster has been identified and proposed to encode the proteins required for GAG biosynthesis. One of these genes, sph3, is predicted to encode a protein belonging to the spherulin 4 family, a protein family with no known function. Construction of an sph3-deficient mutant demonstrated that the gene is necessary for GAG production. To determine the role of Sph3 in GAG biosynthesis, we determined the structure of Aspergillus clavatus Sph3 to 1.25 Å. The structure revealed a (ß/α)8 fold, with similarities to glycoside hydrolase families 18, 27, and 84. Recombinant Sph3 displayed hydrolytic activity against both purified and cell wall-associated GAG. Structural and sequence alignments identified three conserved acidic residues, Asp-166, Glu-167, and Glu-222, that are located within the putative active site groove. In vitro and in vivo mutagenesis analysis demonstrated that all three residues are important for activity. Variants of Asp-166 yielded the greatest decrease in activity suggesting a role in catalysis. This work shows that Sph3 is a glycoside hydrolase essential for GAG production and defines a new glycoside hydrolase family, GH135.
[Mh] Termos MeSH primário: Aspergillus fumigatus/metabolismo
Coccidioidina/química
Proteínas Fúngicas/química
Glicosídeo Hidrolases/química
Polissacarídeos/biossíntese
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Aspergillus fumigatus/enzimologia
Aspergillus fumigatus/patogenicidade
Catálise
Domínio Catalítico
Parede Celular/enzimologia
Coccidioidina/genética
Coccidioidina/fisiologia
Sequência Conservada
Cristalografia por Raios X
Proteínas Fúngicas/genética
Proteínas Fúngicas/fisiologia
Glicosídeo Hidrolases/genética
Glicosídeo Hidrolases/fisiologia
Hidrólise
Dados de Sequência Molecular
Mutação
Polissacarídeos/genética
Conformação Proteica
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Polysaccharides); 0 (galactosaminogalactan); 0 (spherulin); 12622-73-0 (Coccidioidin); EC 3.2.1.- (Glycoside Hydrolases)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150906
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M115.679050


  4 / 121 MEDLINE  
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[PMID]:25979308
[Au] Autor:Wack EE; Ampel NM; Sunenshine RH; Galgiani JN
[Ad] Endereço:Valley Fever Center for Excellence.
[Ti] Título:The Return of Delayed-Type Hypersensitivity Skin Testing for Coccidioidomycosis.
[So] Source:Clin Infect Dis;61(5):787-91, 2015 Sep 01.
[Is] ISSN:1537-6591
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A skin test that detects dermal hypersensitivity in persons with past infection with Coccidioides species is again available for clinical use. Nearly all of the clinical studies with similar materials were published prior to the 1990s, and as a result, many practicing physicians will be unfamiliar with how skin testing for coccidioidomycosis might be useful in patient management or as a research tool. We review clinical and epidemiological studies with past skin test antigens, the composition of past and current skin test preparations with particular attention to differences in the preservatives, and how the current preparation could be used today.
[Mh] Termos MeSH primário: Coccidioidomicose/diagnóstico
Coccidioidomicose/imunologia
Hipersensibilidade Tardia/imunologia
Testes Cutâneos/métodos
[Mh] Termos MeSH secundário: Antígenos de Fungos/imunologia
Coccidioidina/imunologia
Seres Humanos
Micologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Antigens, Fungal); 0 (spherulin); 12622-73-0 (Coccidioidin)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150811
[Lr] Data última revisão:
150811
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150517
[St] Status:MEDLINE
[do] DOI:10.1093/cid/civ388


  5 / 121 MEDLINE  
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[PMID]:22960393
[Au] Autor:Santiso G; Arechavala A; Maiolo E; Balarezo Juncos S; Fernández ML; Messina F; Bianchi M; Negroni R
[Ad] Endereço:Unidad Micología, Hospital de Infecciosas Francisco Javier Muñiz, Buenos Aires, Argentina.
[Ti] Título:[Clinical problems in medical mycology: problem number 45].
[Ti] Título:Problemas clínicos en micología médica: problema número 45..
[So] Source:Rev Iberoam Micol;30(1):72-4, 2013 Jan 03.
[Is] ISSN:2173-9188
[Cp] País de publicação:Spain
[La] Idioma:spa
[Ab] Resumo:A 43 year-old diabetic woman, who suffered chronic cough and brown expectoration, is presented in this clinical problem. X-ray exam and CT thorax scan showed a cavitary lung lesion, located at the upper field of the left lung. This lesion had 5 cm in diameter, with a thick wall and a spherical shadow inside. The diagnosis of chronic cavitary pulmonary coccidioidomycosis was based on the isolation of Coccidioides sp. from cultures of expectoration and bronchoalveolar lavage, and the detection of specific antibodies in immunodiffusion test and counterimmunoelectrophoresis with coccidiodin. Her diabetes was not well controlled. She was treated with intravenous amphotericin B and oral itraconazole, with good clinical response; after four months of treatment the patient abandoned clinical controls. We suppose that the patient presented a coccidioidal fungus ball, inside a chronic cavitary lesion due to pulmonary coccidiodomycosis. She came from an endemic zone of coccidioidomycosis in the Northwest of the Argentine Republic (Catamarca Province).
[Mh] Termos MeSH primário: Coccidioidomicose/diagnóstico
Pneumopatias Fúngicas/diagnóstico
[Mh] Termos MeSH secundário: Adulto
Anfotericina B/administração & dosagem
Anfotericina B/uso terapêutico
Antifúngicos/uso terapêutico
Argentina/epidemiologia
Líquido da Lavagem Broncoalveolar/microbiologia
Coccidioides/isolamento & purificação
Coccidioidina/sangue
Coccidioidomicose/complicações
Coccidioidomicose/diagnóstico por imagem
Coccidioidomicose/tratamento farmacológico
Coccidioidomicose/epidemiologia
Coccidioidomicose/microbiologia
Tosse/etiologia
Diabetes Mellitus Tipo 2/complicações
Quimioterapia Combinada
Dispneia/etiologia
Doenças Endêmicas
Feminino
Seres Humanos
Itraconazol/administração & dosagem
Itraconazol/uso terapêutico
Pneumopatias Fúngicas/complicações
Pneumopatias Fúngicas/diagnóstico por imagem
Pneumopatias Fúngicas/tratamento farmacológico
Pneumopatias Fúngicas/microbiologia
Escarro/microbiologia
Tomografia Computadorizada por Raios X
[Pt] Tipo de publicação:CASE REPORTS; CLINICAL CONFERENCE; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 12622-73-0 (Coccidioidin); 304NUG5GF4 (Itraconazole); 7XU7A7DROE (Amphotericin B)
[Em] Mês de entrada:1307
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120911
[St] Status:MEDLINE


  6 / 121 MEDLINE  
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[PMID]:23065399
[Au] Autor:Pappagianis D; Johnson SM
[Ti] Título:Revision and return of a coccidioidal skin test reagent.
[So] Source:Mycopathologia;174(5-6):351-2, 2012 Dec.
[Is] ISSN:1573-0832
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Mh] Termos MeSH primário: Coccidioidina/imunologia
Coccidioidomicose/diagnóstico
Hipersensibilidade Tardia/diagnóstico
Pneumopatias Fúngicas/diagnóstico
Testes Cutâneos/métodos
[Mh] Termos MeSH secundário: Feminino
Seres Humanos
Masculino
[Pt] Tipo de publicação:COMMENT; EDITORIAL
[Nm] Nome de substância:
12622-73-0 (Coccidioidin)
[Em] Mês de entrada:1305
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121016
[St] Status:MEDLINE
[do] DOI:10.1007/s11046-012-9578-z


  7 / 121 MEDLINE  
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[PMID]:22669545
[Au] Autor:Johnson R; Kernerman SM; Sawtelle BG; Rastogi SC; Nielsen HS; Ampel NM
[Ad] Endereço:David Geffen School of Medicine at UCLA, Kern Medical Center, Bakersfield, CA, USA.
[Ti] Título:A reformulated spherule-derived coccidioidin (Spherusol) to detect delayed-type hypersensitivity in coccidioidomycosis.
[So] Source:Mycopathologia;174(5-6):353-8, 2012 Dec.
[Is] ISSN:1573-0832
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The ability of spherule-derived coccidioidin containing 0.4 % phenol and 0.0001 % thimerosal in buffered saline to induce delayed-type hypersensitivity (DTH) was evaluated in four separate studies. The skin test antigen was titrated in 20 adult volunteers with a recent history of pulmonary coccidioidomycosis using intradermal doses of 0.4, 0.8, and 1.6 µg of antigen, based on total dry weight. Based on these data, a dose of 1.27 µg was shown to elicit a mean ± SEM induration response of 23.5 ± 2.3 mm at 48 h, similar to the 23.6-mm response after 48 h of the U. S. Reference coccidioidin last tested approximately 13 years ago. The 1.27 µg dose in 0.1 mL of the spherule-derived antigen (Spherusol) was then examined in three separate groups of adult volunteers to determine the sensitivity and specificity of the product. Fifty-nine of 60 individuals living in a non-endemic area for coccidioidomycosis were skin test negative to Spherusol. Twelve subjects with a recent history of pulmonary histoplasmosis were skin test negative to Spherusol. Finally, 51 of 52 individuals with a recent diagnosis of acute pulmonary coccidioidomycosis were skin test positive to Spherusol. Within this group, prior therapy with fluconazole did not appear to reduce the reactivity to Spherusol. No serious adverse events were observed in the four studies. From these data, Spherusol was found to be safe and has an overall observed sensitivity and specificity of ≥ 98 % in detecting DTH in coccidioidomycosis.
[Mh] Termos MeSH primário: Coccidioidina/imunologia
Coccidioidomicose/diagnóstico
Hipersensibilidade Tardia/diagnóstico
Pneumopatias Fúngicas/diagnóstico
Testes Cutâneos/métodos
[Mh] Termos MeSH secundário: Adulto
Coccidioidina/administração & dosagem
Coccidioidomicose/imunologia
Feminino
Experimentação Humana
Seres Humanos
Hipersensibilidade Tardia/imunologia
Pneumopatias Fúngicas/imunologia
Masculino
Meia-Idade
Testes Cutâneos/instrumentação
Adulto Jovem
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
12622-73-0 (Coccidioidin)
[Em] Mês de entrada:1305
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120607
[St] Status:MEDLINE
[do] DOI:10.1007/s11046-012-9555-6


  8 / 121 MEDLINE  
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[PMID]:20177972
[Au] Autor:Castañón-Olivares LR; Laniado-Laborín R; Concepcion T; Muñoz-Hernández B; Aroch-Calderón A; Aranda-Uribe IS; Flores-Sánchez MA; del Rocío González-Martínez M; Hernández-Navarez A; Manjarrez-Zavala ME; Miranda-Mauricio S; Palma G; Pérez-Mejía A
[Ad] Endereço:Micología Médica, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico.
[Ti] Título:Clinical comparison of two Mexican coccidioidins.
[So] Source:Mycopathologia;169(6):427-30, 2010 Jun.
[Is] ISSN:1573-0832
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Coccidioidin, an extract from the saprophytic mycelial form of Coccidioides spp., has been a very useful antigen preparation both for skin and serological tests for coccidioidomycosis. Unfortunately, coccidioidin is not currently available for skin testing in the United States. Coccidioidin has been produced commercially in Mexico by a vaccine and reagents laboratory of the Mexican Federal Government. It also has been produced at the Microbiology Laboratory of the Faculty of Medicine, Universidad Nacional Autónoma de México exclusively as an antigen for research projects. The objective of the study was to compare both coccidioidins in their reactivity and safety when applied in humans. One hundred and eighty-four volunteers were tested; median age was 33 (range 14-82). When the cutoff point is set in 5 mm, 88 subjects (47.8%) had a positive test for the commercial coccidioidin and 76 (41.3%; CI(95%) 0.50, 1.15; P = 0.20) were positive with the research antigen. Seventy-five subjects were positive for both antigens and 96 were negative for both. Fifty-nine subjects (31.3%) reported an adverse reaction after the application of the antigen; they were mostly very mild local reactions. Mexican research coccidioidin is a safe and reliable antigen that can be used for the detection of coccidioidomycosis infection in mammals.
[Mh] Termos MeSH primário: Antígenos de Fungos
Coccidioidina
Coccidioidomicose/diagnóstico
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Antígenos de Fungos/administração & dosagem
Antígenos de Fungos/efeitos adversos
Antígenos de Fungos/imunologia
Coccidioides/imunologia
Coccidioidina/administração & dosagem
Coccidioidina/efeitos adversos
Coccidioidina/imunologia
Coccidioidomicose/imunologia
Coccidioidomicose/microbiologia
Feminino
Seres Humanos
Masculino
México
Meia-Idade
Testes Cutâneos
Estados Unidos
Adulto Jovem
[Pt] Tipo de publicação:COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Fungal); 12622-73-0 (Coccidioidin)
[Em] Mês de entrada:1008
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100224
[St] Status:MEDLINE
[do] DOI:10.1007/s11046-010-9280-y


  9 / 121 MEDLINE  
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[PMID]:20157783
[Au] Autor:Ampel NM; Hector RF
[Ad] Endereço:Valley Fever Center for Excellence, University of Arizona, Southern Arizona Veterans Affairs Health Care System, Tucson, AZ, USA. nampel@email.arizona.edu
[Ti] Título:Measuring cellular immunity in coccidioidomycosis: the time is now.
[So] Source:Mycopathologia;169(6):425-6, 2010 Jun.
[Is] ISSN:1573-0832
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Mh] Termos MeSH primário: Coccidioides/imunologia
Coccidioidomicose
Imunidade Celular/imunologia
[Mh] Termos MeSH secundário: Coccidioidina/imunologia
Coccidioidomicose/diagnóstico
Coccidioidomicose/imunologia
Seres Humanos
Hipersensibilidade Tardia/imunologia
México
Testes Cutâneos/métodos
[Pt] Tipo de publicação:COMMENT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (spherulin); 12622-73-0 (Coccidioidin)
[Em] Mês de entrada:1008
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100217
[St] Status:MEDLINE
[do] DOI:10.1007/s11046-010-9285-6


  10 / 121 MEDLINE  
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[PMID]:16463089
[Au] Autor:Ampel NM; Hector RF; Lindan CP; Rutherford GW
[Ad] Endereço:Department of Medicine , University of Arizona, Tucson, AZ, USA.
[Ti] Título:An archived lot of coccidioidin induces specific coccidioidal delayed-type hypersensitivity and correlates with in vitro assays of coccidioidal cellular immune response.
[So] Source:Mycopathologia;161(2):67-72, 2006 Feb.
[Is] ISSN:0301-486X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:No test for assessing cellular immune response in coccidioidomycosis is currently available in the United States. In the present study, we tested 49 healthy subjects living in the coccidioidal endemic region with a 1:55.8 dilution of a single lot of coccidioidin archived since the 1970s. In this group, 23 evaluable subjects demonstrated >/=5 mm of induration at 24, 48 or 72 h, with a mean+/-SEM maximum induration of 18.4+/-4.0 mm. The induration results among 14 subjects reactive at 24 h were compared to those from 179 individuals in an historical cohort studied in the 1980s using a reference lot of coccidioidin. Results were within 5% and not significantly different (P=0.924). The maximum induration response of all evaluable subjects correlated significantly with the results of in vitro tests of coccidioidal cellular immunity using supernatant interferon-gamma concentration and CD69 expression on T cells (Spearman rank correlation coefficients 0.69 and 0.68, respectively; P<0.01 for both). These data suggest that archived coccidioidin retains its potency and specificity and that in vitro test of coccidioidal immunity may have utility in the measurement of coccidioidal cellular immunity.
[Mh] Termos MeSH primário: Coccidioides/imunologia
Coccidioidina/efeitos adversos
Coccidioidomicose/diagnóstico
Hipersensibilidade Tardia/imunologia
Testes Cutâneos/métodos
[Mh] Termos MeSH secundário: Adolescente
Adulto
Anticorpos Antifúngicos/sangue
Coccidioidina/imunologia
Coccidioidomicose/imunologia
Coccidioidomicose/microbiologia
Seres Humanos
Imunidade Celular/imunologia
Interferon gama/sangue
Meia-Idade
Testes Cutâneos/efeitos adversos
[Pt] Tipo de publicação:CLINICAL TRIAL, PHASE I; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Antibodies, Fungal); 12622-73-0 (Coccidioidin); 82115-62-6 (Interferon-gamma)
[Em] Mês de entrada:0605
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:060208
[St] Status:MEDLINE



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