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[PMID]: | 27757838 |
[Au] Autor: | Aziz F; Gao W; Yan Q |
[Ad] Endereço: | Department of Biochemistry and Molecular Biology, Liaoning Provincial Core Laboratory of Glycobiology and Glycoengineering, Dalian Medical University, Dalian, People's Republic of China. |
[Ti] Título: | Fucosyltransferase-4 and Oligosaccharide Lewis Y Antigen as potentially Correlative Biomarkers of Helicobacter pylori CagA Associated Gastric Cancer. |
[So] Source: | Pathol Oncol Res;23(1):173-179, 2017 Jan. | [Is] ISSN: | 1532-2807 |
[Cp] País de publicação: | Netherlands |
[La] Idioma: | eng |
[Ab] Resumo: | H. pylori cytotoxin associated antigen A (CagA) plays a significant role in the progression of gastric cancer but their effect on fucosylation to develop gastric cancer is unknown. Fucosyltransferase IV (FUT4) is the key enzyme for synthesis of LewisY (LeY) carried by glycoproteins and glycolipids on the cell membrane. Herein, we compare the expression of CagA, p-EGFR, FUT4 and LeY in gastritis (n = 128, 176), gastric ulcer (n = 174, 213), and gastric cancer (n = 323, 261) tissue and serum samples, respectively by IHC and ELISA. Moreover, we investigated the potential correlation of CagA with FUT4 and LeY overexpression through EGFR activation. IHC and ELISA results showed higher positive cases of H. pylori CagA (83, 86 %), p-EGFR (81, 72 %), FUT4 (91, 97 %) and LeY (93, 92 %) in gastric cancer, compared to gastritis and gastric ulcer, H. pylori CagA (58, 67 & 59, 73 %), p-EGFR (52, 63 & 35, 47 %), FUT4 (68, 78 & 67, 82 %) and LeY (62,76 & 65, 85 %), respectively. We found a significant high expression (H-Value) of CagA (1.79, 1.66), p-EGFR (1.53, 1.58), FUT4 (2.14, 1.66) and LeY (1.69, 1.61) in gastric cancer tissues and serum, respectively as compared to chronic gastritis and gastric ulcers, CagA (0.64,1.14), p-EGFR (0.856, 0.678), FUT4 (0.949,1.197) and LeY (0.68,1.008) (P < 0.0001), respectively. Furthermore, H. pylori CagA showed significant correlation with p-EGFR (R-0.62, -0.74), FUT4 (R-0.81, -0.76) and LeY (R-0.82, -0.70) in gastric tissues and serum (P < 0.0001). H. pylori CagA plays key role in the development of gastric cancer with overexpression of FUT4/LeY, serve as potentially correlative biomarkers of H. pylori CagA associated gastric cancer. |
[Mh] Termos MeSH primário: |
Biomarcadores Tumorais/metabolismo Fucosiltransferases/metabolismo Helicobacter pylori/patogenicidade Sistema do Grupo Sanguíneo de Lewis/metabolismo Antígeno Lewis X/metabolismo Oligossacarídeos/metabolismo Neoplasias Gástricas/metabolismo Neoplasias Gástricas/microbiologia
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[Mh] Termos MeSH secundário: |
Feminino Gastrite/metabolismo Gastrite/microbiologia Infecções por Helicobacter/metabolismo Infecções por Helicobacter/microbiologia Seres Humanos Masculino Meia-Idade
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Biomarkers, Tumor); 0 (Lewis Blood-Group System); 0 (Lewis X Antigen); 0 (Lewis Y antigen); 0 (Oligosaccharides); EC 2.4.1.- (FUT4 protein, human); EC 2.4.1.- (Fucosyltransferases) |
[Em] Mês de entrada: | 1703 |
[Cu] Atualização por classe: | 171116 |
[Lr] Data última revisão:
| 171116 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 161021 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1007/s12253-016-0122-1 |
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