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[PMID]: | 28087664 |
[Au] Autor: | Yin X; Yu H; Jin X; Li J; Guo H; Shi Q; Yin Z; Xu Y; Wang X; Liu R; Wang S; Zhang L |
[Ad] Endereço: | Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China. |
[Ti] Título: | Human Blood CD1c+ Dendritic Cells Encompass CD5high and CD5low Subsets That Differ Significantly in Phenotype, Gene Expression, and Functions. |
[So] Source: | J Immunol;198(4):1553-1564, 2017 Feb 15. | [Is] ISSN: | 1550-6606 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | There are three major dendritic cell (DC) subsets in both humans and mice, that is, plasmacytoid DCs and two types of conventional DCs (cDCs), cDC1s and cDC2s. cDC2s are important for polarizing CD4 naive T cells into different subsets, including Th1, Th2, Th17, Th22, and regulatory T cells. In mice, cDC2s can be further divided into phenotypically and functionally distinct subgroups. However, subsets of human cDC2s have not been reported. In the present study, we showed that human blood CD1c cDCs (cDC2s) can be further separated into two subpopulations according to their CD5 expression status. Comparative transcriptome analyses showed that the CD5 DCs expressed higher levels of cDC2-specific genes, including IFN regulatory factor 4, which is essential for the cDC2 development and its migration to lymph nodes. In contrast, CD5 DCs preferentially expressed monocyte-related genes, including the lineage-specific transcription factor MAFB. Furthermore, compared with the CD5 subpopulation, the CD5 subpopulation showed stronger migration toward CCL21 and overrepresentation among migratory DCs in lymph nodes. Additionally, the CD5 DCs induced naive T cell proliferation more potently than did the CD5 DCs. Moreover, CD5 DCs induced higher levels of IL-10-, IL-22-, and IL-4-producing T cell formation, whereas CD5 DCs induced higher levels of IFN-γ-producing T cell formation. Thus, we show that human blood CD1c cDC2s encompass two subsets that differ significantly in phenotype, that is, gene expression and functions. We propose that these two subsets of human cDC2s could potentially play contrasting roles in immunity or tolerance. |
[Mh] Termos MeSH primário: |
Antígenos CD1/imunologia Antígenos CD5/genética Células Dendríticas/imunologia Células Dendríticas/fisiologia Glicoproteínas/imunologia
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[Mh] Termos MeSH secundário: |
Antígenos CD1/genética Células Sanguíneas/imunologia Antígenos CD5/análise Diferenciação Celular/efeitos dos fármacos Quimiocina CCL21/farmacologia Células Dendríticas/classificação Células Dendríticas/efeitos dos fármacos Glicoproteínas/genética Seres Humanos Tolerância Imunológica Fatores Reguladores de Interferon/genética Fatores Reguladores de Interferon/imunologia Interleucina-10/imunologia Interleucina-4/biossíntese Interleucina-4/imunologia Interleucinas/biossíntese Interleucinas/imunologia Ativação Linfocitária Fenótipo Linfócitos T Auxiliares-Indutores/imunologia Linfócitos T Reguladores/imunologia Células Th1/imunologia
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Antigens, CD1); 0 (CCL21 protein, human); 0 (CD1C protein, human); 0 (CD5 Antigens); 0 (Chemokine CCL21); 0 (Glycoproteins); 0 (IL10 protein, human); 0 (Interferon Regulatory Factors); 0 (Interleukins); 0 (interferon regulatory factor-4); 0 (interleukin-22); 130068-27-8 (Interleukin-10); 207137-56-2 (Interleukin-4) |
[Em] Mês de entrada: | 1708 |
[Cu] Atualização por classe: | 171116 |
[Lr] Data última revisão:
| 171116 |
[Sb] Subgrupo de revista: | AIM; IM |
[Da] Data de entrada para processamento: | 170115 |
[St] Status: | MEDLINE |
[do] DOI: | 10.4049/jimmunol.1600193 |
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