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  1 / 11822 MEDLINE  
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[PMID]:28910369
[Au] Autor:Kumar NP; Moideen K; Viswanathan V; Sivakumar S; Menon PA; Kornfeld H; Babu S
[Ad] Endereço:National Institutes of Health-NIRT-International Center for Excellence in Research, Chennai, India.
[Ti] Título:Heightened circulating levels of antimicrobial peptides in tuberculosis-Diabetes co-morbidity and reversal upon treatment.
[So] Source:PLoS One;12(9):e0184753, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The association of antimicrobial peptides (AMPs) with tuberculosis-diabetes comorbidity (PTB-DM) is not well understood. METHODS: To study the association of AMPs with PTB-DM, we examined the systemic levels of cathelicidin (LL37), human beta defensin- 2 (HBD2), human neutrophil peptides 1-3, (HNP1-3) and granulysin in individuals with either PTB-DM, PTB, latent TB (LTB) or no TB infection (NTB). RESULTS: Circulating levels of cathelicidin and HBD2 were significantly higher and granulysin levels were significantly lower in PTB-DM compared to PTB, LTB or NTB, while the levels of HNP1-3 were significantly higher in PTB-DM compared to LTB or NTB individuals. Moreover, the levels of cathelicidin and/or HBD2 were significantly higher in PTB-DM or PTB individuals with bilateral and cavitary disease and also exhibited a significant positive relationship with bacterial burden. Cathelidin, HBD2 and HNP1-3 levels exhibited a positive relationship with HbA1c and/or fasting blood glucose levels. Finally, anti-tuberculosis therapy resulted in significantly diminished levels of cathelicidin, HBD2, granulysin and significantly enhanced levels of HNP1-3 and granulysin in PTB-DM and/or PTB individuals. CONCLUSION: Therefore, our data demonstrate that PTB-DM is associated with markedly enhanced levels of AMPs and diminished levels of granulysin.
[Mh] Termos MeSH primário: Peptídeos Catiônicos Antimicrobianos/sangue
Antituberculosos/uso terapêutico
Diabetes Mellitus Tipo 2/metabolismo
Tuberculose Pulmonar/tratamento farmacológico
alfa-Defensinas/sangue
[Mh] Termos MeSH secundário: Adulto
Idoso
Antígenos de Diferenciação de Linfócitos T/sangue
Catelicidinas/sangue
Comorbidade
Feminino
Seres Humanos
Masculino
Meia-Idade
Tuberculose Pulmonar/metabolismo
Adulto Jovem
beta-Defensinas/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Differentiation, T-Lymphocyte); 0 (Antimicrobial Cationic Peptides); 0 (Antitubercular Agents); 0 (Cathelicidins); 0 (DEFB4A protein, human); 0 (GNLY protein, human); 0 (alpha-Defensins); 0 (beta-Defensins); 0 (cathelicidin antimicrobial peptide); 0 (human neutrophil peptide 1)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170915
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184753


  2 / 11822 MEDLINE  
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[PMID]:28872666
[Au] Autor:Gurevich I; Feferman T; Milo I; Tal O; Golani O; Drexler I; Shakhar G
[Ad] Endereço:Department of Immunology and Veterinary Services, Weizmann Institute of Science, Rehovot, Israel.
[Ti] Título:Active dissemination of cellular antigens by DCs facilitates CD8 T-cell priming in lymph nodes.
[So] Source:Eur J Immunol;47(10):1802-1818, 2017 Oct.
[Is] ISSN:1521-4141
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Antigen (Ag) specific activation of naïve T cells by migrating dendritic cells (DCs) is a highly efficient process, although the chances for their colocalization in lymph nodes (LNs) appear low. Ag presentation may be delegated from Ag-donor DCs to the abundant resident DCs, but the routes of Ag transfer and how it facilitates T-cell activation remain unclear. We visualized CD8 T cell-DC interactions to study the sites, routes, and cells mediating Ag transfer in mice. In vitro, Ag transfer from isolated ovalbumin (OVA) bone marrow (BM) DCs triggered widespread arrest, Ca flux, and CD69 upregulation in OT-I T cells contacting recipient DCs. Intravital two-photon imaging revealed that survival of Ag-donor DCs in LNs was required for Ag dissemination among resident CD11c DCs. Upon interaction with recipient DCs, CD8 T cells clustered, upregulated CD69, proliferated and differentiated into effectors. Few DCs sufficed for activation, and for efficient Ag dissemination lymphocyte function associated antigen 1 (LFA-1) expression on recipient DCs was essential. Similar findings characterized DCs infected with a replication-deficient OVA-expressing Vaccinia virus known to downregulate MHC-I. Overall, active Ag dissemination from live incoming DCs helped activate CD8 T cells by increasing the number of effective presenting cells and salvaged T-cell priming when Ag-donor DCs could not present Ag.
[Mh] Termos MeSH primário: Antígenos/metabolismo
Linfócitos T CD8-Positivos/imunologia
Apresentação Cruzada
Células Dendríticas/imunologia
Linfonodos/imunologia
[Mh] Termos MeSH secundário: Animais
Apresentação do Antígeno
Antígenos/imunologia
Antígenos CD/genética
Antígenos CD/imunologia
Antígenos de Diferenciação de Linfócitos T/genética
Antígenos de Diferenciação de Linfócitos T/imunologia
Linfócitos T CD8-Positivos/metabolismo
Movimento Celular
Células Dendríticas/química
Células Dendríticas/metabolismo
Microscopia Intravital
Lectinas Tipo C/genética
Lectinas Tipo C/imunologia
Linfonodos/citologia
Ativação Linfocitária
Camundongos
Camundongos Endogâmicos C57BL
Ovalbumina/genética
Ovalbumina/imunologia
Vírus Vaccinia/genética
Vírus Vaccinia/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens); 0 (Antigens, CD); 0 (Antigens, Differentiation, T-Lymphocyte); 0 (CD69 antigen); 0 (Lectins, C-Type); 9006-59-1 (Ovalbumin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170906
[St] Status:MEDLINE
[do] DOI:10.1002/eji.201747042


  3 / 11822 MEDLINE  
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[PMID]:28750012
[Au] Autor:Kang SJ; Jin HM; Cho YN; Kim SE; Kim UJ; Park KH; Jang HC; Jung SI; Kee SJ; Park YW
[Ad] Endereço:Department of Infectious Diseases, Chonnam National University Medical School and Hospital, Gwangju, Republic of Korea.
[Ti] Título:Increased level and interferon-γ production of circulating natural killer cells in patients with scrub typhus.
[So] Source:PLoS Negl Trop Dis;11(7):e0005815, 2017 Jul.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Natural killer (NK) cells are essential immune cells against several pathogens. Not much is known regarding the roll of NK cells in Orientia tsutsugamushi infection. Thus, this study aims to determine the level, function, and clinical relevance of NK cells in patients with scrub typhus. METHODOLOGY/PRINCIPAL FINDINGS: This study enrolled fifty-six scrub typhus patients and 56 health controls (HCs). The patients were divided into subgroups according to their disease severity. A flow cytometry measured NK cell level and function in peripheral blood. Circulating NK cell levels and CD69 expressions were significantly increased in scrub typhus patients. Increased NK cell levels reflected disease severity. In scrub typhus patients, tests showed their NK cells produced higher amounts of interferon (IFN)-γ after stimulation with interleukin (IL)-12 and IL-18 relative to those of HCs. Meanwhile, between scrub typhus patients and HCs, the cytotoxicity and degranulation of NK cells against K562 were comparable. CD69 expressions were recovered to the normal levels in the remission phase. CONCLUSIONS: This study shows that circulating NK cells are activated and numerically increased, and they produced more IFN-γ in scrub typhus patients.
[Mh] Termos MeSH primário: Interferon gama/imunologia
Células Matadoras Naturais/imunologia
Tifo por Ácaros/imunologia
[Mh] Termos MeSH secundário: Idoso
Antígenos CD/metabolismo
Antígenos de Diferenciação de Linfócitos T/metabolismo
Estudos de Casos e Controles
Feminino
Citometria de Fluxo
Seres Humanos
Interleucina-12/farmacologia
Interleucina-18/farmacologia
Lectinas Tipo C/metabolismo
Contagem de Leucócitos
Ativação Linfocitária
Masculino
Meia-Idade
Orientia tsutsugamushi
Índice de Gravidade de Doença
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD); 0 (Antigens, Differentiation, T-Lymphocyte); 0 (CD69 antigen); 0 (Interleukin-18); 0 (Lectins, C-Type); 187348-17-0 (Interleukin-12); 82115-62-6 (Interferon-gamma)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005815


  4 / 11822 MEDLINE  
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[PMID]:28730595
[Au] Autor:Gao J; Zheng Q; Xin N; Wang W; Zhao C
[Ad] Endereço:Department of Pathophysiology, College of Basic Medical Science, China Medical University, Shenyang, China.
[Ti] Título:CD155, an onco-immunologic molecule in human tumors.
[So] Source:Cancer Sci;108(10):1934-1938, 2017 Oct.
[Is] ISSN:1349-7006
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CD155 is the fifth member in the nectin-like molecule family, and functions as the receptor of poliovirus; therefore, CD155 is also referred to as necl-5, or PVR. As an immunoglobulin-like adhesion molecule, CD155 is involved in cell motility, and natural killer and T cell-mediated immunity. CD155 is barely or weakly expressed in various normal human tissues, but frequently overexpressed in human malignant tumors. CD155 overexpression promotes tumor cell invasion and migration, and is associated with tumor progression and poor prognosis. As the ligand for both costimulatory receptor CD226 and coinhibitory receptor TIGIT and CD96 on natural killer and T cells, CD155 seems to play a dual role in oncoimmunity. However, some recent studies indicate that CD155 overexpression may induce tumor immune escape. Taken together, CD155 may be considered as a target for the treatment of tumors with CD155 overexpression.
[Mh] Termos MeSH primário: Células T Matadoras Naturais/imunologia
Neoplasias/imunologia
Receptores Virais/metabolismo
Regulação para Cima
[Mh] Termos MeSH secundário: Animais
Antígenos de Diferenciação de Linfócitos T/metabolismo
Movimento Celular
Progressão da Doença
Regulação Neoplásica da Expressão Gênica
Seres Humanos
Imunidade Celular
Invasividade Neoplásica
Neoplasias/metabolismo
Prognóstico
Receptores Imunológicos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antigens, Differentiation, T-Lymphocyte); 0 (CD226 antigen); 0 (Receptors, Immunologic); 0 (Receptors, Virus); 0 (TIGIT protein, human); 0 (poliovirus receptor)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170722
[St] Status:MEDLINE
[do] DOI:10.1111/cas.13324


  5 / 11822 MEDLINE  
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[PMID]:28701399
[Au] Autor:Hensel MT; Peng T; Cheng A; De Rosa SC; Wald A; Laing KJ; Jing L; Dong L; Magaret AS; Koelle DM
[Ad] Endereço:Department of Global Health, Program in Pathobiology, University of Washington, Seattle, Washington, USA.
[Ti] Título:Selective Expression of CCR10 and CXCR3 by Circulating Human Herpes Simplex Virus-Specific CD8 T Cells.
[So] Source:J Virol;91(19), 2017 Oct 01.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Herpes simplex virus (HSV) infection is restricted to epithelial cells and neurons and is controlled by CD8 T cells. These cells both traffic to epithelial sites of recurrent lytic infection and to ganglia and persist at the dermal-epidermal junction for up to 12 weeks after lesion resolution. We previously showed that cutaneous lymphocyte-associated antigen (CLA), a functional E-selectin ligand (ESL), is selectively expressed on circulating HSV-2-specific CD8 T cells. CLA/ESL mediates adhesion of T cells to inflamed vascular endothelium. Later stages in T-cell homing involve chemokines (Ch) and lymphocyte chemokine receptors (ChR) for vascular wall arrest and diapedesis. Several candidate ChR have been implicated in skin homing. We measured cell surface ChR on HSV-specific human peripheral blood CD8 T cells and extended our studies to HSV-1. We observed preferential cell surface expression of CCR10 and CXCR3 by HSV-specific CD8 T cells compared to CD8 T cells specific for control viruses, Epstein-Barr virus (EBV) and cytomegalovirus (CMV), and compared to bulk memory CD8 T cells. CXCR3 ligand mRNA levels were selectively increased in skin biopsy specimens from persons with recurrent HSV-2, while the mRNA levels of the CCR10 ligand CCL27 were equivalent in lesion and control skin. Our data are consistent with a model in which CCL27 drives baseline recruitment of HSV-specific CD8 T cells expressing CCR10, while interferon-responsive CXCR3 ligands recruit additional cells in response to virus-driven inflammation. HSV-2 causes very localized recurrent infections in the skin and genital mucosa. Virus-specific CD8 T cells home to the site of recurrent infection and participate in viral clearance. The exit of T cells from the blood involves the use of chemokine receptors on the T-cell surface and chemokines that are present in infected tissue. In this study, circulating HSV-2-specific CD8 T cells were identified using specific fluorescent tetramer reagents, and their expression of several candidate skin-homing-associated chemokine receptors was measured using flow cytometry. We found that two chemokine receptors, CXCR3 and CCR10, are upregulated on HSV-specific CD8 T cells in blood. The chemokines corresponding to these receptors are also expressed in infected tissues. Vaccine strategies to prime CD8 T cells to home to HSV lesions should elicit these chemokine receptors if possible to increase the homing of vaccine-primed cells to sites of infection.
[Mh] Termos MeSH primário: Linfócitos T CD8-Positivos/imunologia
Quimiocina CCL27/imunologia
Herpes Simples/imunologia
Herpesvirus Humano 2/imunologia
Ativação Linfocitária/imunologia
Receptores CCR10/imunologia
Receptores CXCR3/imunologia
[Mh] Termos MeSH secundário: Antígenos de Diferenciação de Linfócitos T/imunologia
Linfócitos T CD8-Positivos/metabolismo
Quimiocina CCL27/genética
Citomegalovirus/imunologia
Feminino
Citometria de Fluxo
Herpes Simples/virologia
Herpesvirus Humano 4/imunologia
Seres Humanos
Memória Imunológica/imunologia
Masculino
Glicoproteínas de Membrana/imunologia
RNA Mensageiro/genética
Receptores CCR10/biossíntese
Receptores CCR10/genética
Receptores CXCR3/biossíntese
Receptores CXCR3/genética
Pele/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Differentiation, T-Lymphocyte); 0 (CCL27 protein, human); 0 (CCR10 protein, human); 0 (CTAGE1 protein, human); 0 (CXCR3 protein, human); 0 (Chemokine CCL27); 0 (Membrane Glycoproteins); 0 (RNA, Messenger); 0 (Receptors, CCR10); 0 (Receptors, CXCR3)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170714
[St] Status:MEDLINE


  6 / 11822 MEDLINE  
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[PMID]:28575063
[Au] Autor:Rani M; Schwacha MG
[Ad] Endereço:Department of Surgery, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States of America.
[Ti] Título:The composition of T-cell subsets are altered in the burn wound early after injury.
[So] Source:PLoS One;12(6):e0179015, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Burn-induced inflammation leads to impaired immune responses resulting in increased morbidity and mortality. T-cells are central in the immune response and circulating CD4 and CD8 T-cells have been used to evaluate immune status; however, the role of these T-cell subsets in the burn wound is unknown. METHODS: Male C57BL/6 mice were subjected to a major 3rd degree scald burn or sham treatment. Twenty-four hours later, full thickness skin samples from sham mice and the burn wounds were collected and single cells were isolated and analyzed for αß TCR, γδ TCR, CD3, CD4, CD8 and CD69 expressions by flow cytometry. RESULTS: The burn wound contained significantly greater numbers of T-cells than skin from sham mice, due to a profound infiltration of αß T-cells. These infiltrating αß T-cells were primarily suppressor T-cells with a CD8+ or CD8-CD4- phenotype. The 15-fold increase in CD8+ αß T-cells caused a decrease in the CD4:CD8 ratio from 0.7 in sham skin to 0.3 in the burn wound. In contrast, the majority of the γδ T-cells in sham skin were CD4-CD8-, which decreased 9-fold in the burn wound. CD69 expression was suppressed on burn wound αß T-cells, but increased on γδ T-cells in the burn wound. CONCLUSIONS: The infiltrating burn wound αß T-cells likely act to quell inflammation. In contrast wound γδ T-cells were activated with elevated CD4 and CD69 expression. Thus, these two distinct T-cell subsets likely differentially regulate the burn wound inflammatory response.
[Mh] Termos MeSH primário: Queimaduras/patologia
Pele/patologia
Subpopulações de Linfócitos T/patologia
[Mh] Termos MeSH secundário: Animais
Antígenos CD/análise
Antígenos CD/imunologia
Antígenos de Diferenciação de Linfócitos T/análise
Antígenos de Diferenciação de Linfócitos T/imunologia
Queimaduras/imunologia
Complexo CD3/análise
Complexo CD3/imunologia
Antígenos CD4/análise
Antígenos CD4/imunologia
Linfócitos T CD4-Positivos/imunologia
Linfócitos T CD4-Positivos/patologia
Antígenos CD8/análise
Antígenos CD8/imunologia
Linfócitos T CD8-Positivos/imunologia
Linfócitos T CD8-Positivos/patologia
Lectinas Tipo C/análise
Lectinas Tipo C/imunologia
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Receptores de Antígenos de Linfócitos T alfa-beta/análise
Receptores de Antígenos de Linfócitos T alfa-beta/imunologia
Receptores de Antígenos de Linfócitos T gama-delta/análise
Receptores de Antígenos de Linfócitos T gama-delta/imunologia
Pele/imunologia
Subpopulações de Linfócitos T/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD); 0 (Antigens, Differentiation, T-Lymphocyte); 0 (CD3 Complex); 0 (CD4 Antigens); 0 (CD69 antigen); 0 (CD8 Antigens); 0 (Lectins, C-Type); 0 (Receptors, Antigen, T-Cell, alpha-beta); 0 (Receptors, Antigen, T-Cell, gamma-delta)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170603
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179015


  7 / 11822 MEDLINE  
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[PMID]:28541707
[Au] Autor:Liu Q; Shi Q; Marcoux D; Batt DG; Cornelius L; Qin LY; Ruan Z; Neels J; Beaudoin-Bertrand M; Srivastava AS; Li L; Cherney RJ; Gong H; Watterson SH; Weigelt C; Gillooly KM; McIntyre KW; Xie JH; Obermeier MT; Fura A; Sleczka B; Stefanski K; Fancher RM; Padmanabhan S; Rp T; Kundu I; Rajareddy K; Smith R; Hennan JK; Xing D; Fan J; Levesque PC; Ruan Q; Pitt S; Zhang R; Pedicord D; Pan J; Yarde M; Lu H; Lippy J; Goldstine C; Skala S; Rampulla RA; Mathur A; Gupta A; Arunachalam PN; Sack JS; Muckelbauer JK; Cvijic ME; Salter-Cid LM
[Ad] Endereço:Research & Development, Bristol-Myers Squibb Company , Route 206 and Province Line Road, Princeton, New Jersey 08543, United States.
[Ti] Título:Identification of a Potent, Selective, and Efficacious Phosphatidylinositol 3-Kinase δ (PI3Kδ) Inhibitor for the Treatment of Immunological Disorders.
[So] Source:J Med Chem;60(12):5193-5208, 2017 Jun 22.
[Is] ISSN:1520-4804
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PI3Kδ plays an important role controlling immune cell function and has therefore been identified as a potential target for the treatment of immunological disorders. This article highlights our work toward the identification of a potent, selective, and efficacious PI3Kδ inhibitor. Through careful SAR, the successful replacement of a polar pyrazole group by a simple chloro or trifluoromethyl group led to improved Caco-2 permeability, reduced Caco-2 efflux, reduced hERG PC activity, and increased selectivity profile while maintaining potency in the CD69 hWB assay. The optimization of the aryl substitution then identified a 4'-CN group that improved the human/rodent correlation in microsomal metabolic stability. Our lead molecule is very potent in PK/PD assays and highly efficacious in a mouse collagen-induced arthritis model.
[Mh] Termos MeSH primário: Artrite Experimental/tratamento farmacológico
Avaliação Pré-Clínica de Medicamentos/métodos
Inibidores Enzimáticos/farmacologia
Fosfatidilinositol 3-Quinases/antagonistas & inibidores
Relação Estrutura-Atividade
[Mh] Termos MeSH secundário: Animais
Antígenos CD/metabolismo
Antígenos de Diferenciação de Linfócitos T/metabolismo
Células CACO-2/efeitos dos fármacos
Células CACO-2/imunologia
Cães
Canal de Potássio ERG1/metabolismo
Inibidores Enzimáticos/química
Feminino
Seres Humanos
Doenças do Sistema Imune/tratamento farmacológico
Isoenzimas/antagonistas & inibidores
Isoenzimas/metabolismo
Lectinas Tipo C/metabolismo
Masculino
Camundongos Endogâmicos BALB C
Pirazóis/química
Pirazóis/metabolismo
Pirazóis/farmacologia
Coelhos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD); 0 (Antigens, Differentiation, T-Lymphocyte); 0 (CD69 antigen); 0 (ERG1 Potassium Channel); 0 (Enzyme Inhibitors); 0 (Isoenzymes); 0 (KCNH2 protein, human); 0 (Lectins, C-Type); 0 (Pyrazoles); EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170811
[Lr] Data última revisão:
170811
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jmedchem.7b00618


  8 / 11822 MEDLINE  
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[PMID]:28539453
[Au] Autor:Bolduc JF; Hany L; Barat C; Ouellet M; Tremblay MJ
[Ad] Endereço:Axe des Maladies Infectieuses et Immunitaires, Centre de Recherche du Centre Hospitalier Universitaire de Québec-Université Laval, Pavillon CHUL, Québec (QC), Canada.
[Ti] Título:Epigenetic Metabolite Acetate Inhibits Class I/II Histone Deacetylases, Promotes Histone Acetylation, and Increases HIV-1 Integration in CD4 T Cells.
[So] Source:J Virol;91(16), 2017 Aug 15.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, we investigated the effect of acetate, the most concentrated short-chain fatty acid (SCFA) in the gut and bloodstream, on the susceptibility of primary human CD4 T cells to HIV-1 infection. We report that HIV-1 replication is increased in CD3/CD28-costimulated CD4 T cells upon acetate treatment. This enhancing effect correlates with increased expression of the early activation marker CD69 and impaired class I/II histone deacetylase (HDAC) activity. In addition, acetate enhances acetylation of histones H3 and H4 and augments HIV-1 integration into the genome of CD4 T cells. Thus, we propose that upon antigen presentation, acetate influences class I/II HDAC activity that transforms condensed chromatin into a more relaxed structure. This event leads to a higher level of viral integration and enhanced HIV-1 production. In line with previous studies showing reactivation of latent HIV-1 by SCFAs, we provide evidence that acetate can also increase the susceptibility of primary human CD4 T cells to productive HIV-1 infection. Alterations in the fecal microbiota and intestinal epithelial damage involved in the gastrointestinal disorder associated with HIV-1 infection result in microbial translocation that leads to disease progression and virus-related comorbidities. Indeed, notably via production of short-chain fatty acids, bacteria migrating from the lumen to the intestinal mucosa could influence HIV-1 replication by epigenetic regulatory mechanisms, such as histone acetylation. We demonstrate that acetate enhances virus production in primary human CD4 T cells. Moreover, we report that acetate impairs class I/II histone deacetylase activity and increases integration of HIV-1 DNA into the host genome. Therefore, it can be postulated that bacterial metabolites such as acetate modulate HIV-1-mediated disease progression.
[Mh] Termos MeSH primário: Acetatos/metabolismo
Linfócitos T CD4-Positivos/virologia
HIV-1/fisiologia
Inibidores de Histona Desacetilases/metabolismo
Histonas/metabolismo
Processamento de Proteína Pós-Traducional
Integração Viral
[Mh] Termos MeSH secundário: Acetilação
Antígenos CD/análise
Antígenos de Diferenciação de Linfócitos T/análise
Linfócitos T CD4-Positivos/química
Linfócitos T CD4-Positivos/efeitos dos fármacos
Células Cultivadas
Seres Humanos
Lectinas Tipo C/análise
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Antigens, CD); 0 (Antigens, Differentiation, T-Lymphocyte); 0 (CD69 antigen); 0 (Histone Deacetylase Inhibitors); 0 (Histones); 0 (Lectins, C-Type)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE


  9 / 11822 MEDLINE  
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Antunes, Edson
Texto completo
[PMID]:28489854
[Au] Autor:Braga FG; Ruas LP; Pereira RM; Lima XT; Antunes E; Mamoni RL; Blotta MHSL
[Ad] Endereço:Department of Clinical Pathology, Faculty of Medical Sciences, State University of Campinas, Campinas, São Paulo, Brazil.
[Ti] Título:Functional and phenotypic evaluation of eosinophils from patients with the acute form of paracoccidioidomycosis.
[So] Source:PLoS Negl Trop Dis;11(5):e0005601, 2017 May.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Eosinophilia is a typical finding of the acute/juvenile form of paracoccidioidomycosis (PCM), a systemic mycosis endemic in Latin America. This clinical form is characterized by depressed cellular immune response and production of Th2 cytokines. Moreover, it has been shown that the increased number of eosinophils in peripheral blood of patients returns to normal values after antifungal treatment. However, the role of eosinophils in PCM has never been evaluated. This study aimed to assess the phenotypic and functional characteristics of eosinophils in PCM. METHODS/PRINCIPAL FINDINGS: In 15 patients with the acute form of the disease, we detected expression of MBP, CCL5 (RANTES) and CCL11 (eotaxin) in biopsies of lymph nodes and liver. In addition, there were higher levels of chemokines and granule proteins in the peripheral blood of patients compared to controls. Isolation of eosinophils from blood revealed a higher frequency of CD69+ and TLR2+ eosinophils in patients compared to controls, and a lower population of CD80+ cells. We also evaluated the fungicidal capacity of eosinophils in vitro. Our results revealed that eosinophils from PCM patients and controls exhibit similar ability to kill P. brasiliensis yeast cells, although eosinophils of patients were less responsive to IL-5 stimulation than controls. CONCLUSION/PRINCIPAL FINDINGS: In conclusion, we suggest that eosinophils might play a role in the host response to fungi and in the pathophysiology of PCM by inducing an intense and systemic inflammatory response in the initial phase of the infection.
[Mh] Termos MeSH primário: Eosinofilia/patologia
Eosinófilos/imunologia
Paracoccidioides/imunologia
Paracoccidioidomicose/complicações
Paracoccidioidomicose/patologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Antígenos CD/análise
Antígenos de Diferenciação de Linfócitos T/análise
Antígeno B7-1/análise
Criança
Pré-Escolar
Citocinas/sangue
Eosinófilos/química
Feminino
Seres Humanos
Lectinas Tipo C/análise
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD); 0 (Antigens, Differentiation, T-Lymphocyte); 0 (B7-1 Antigen); 0 (CD69 antigen); 0 (Cytokines); 0 (Lectins, C-Type)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170511
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005601


  10 / 11822 MEDLINE  
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[PMID]:28475283
[Au] Autor:Cibrián D; Sánchez-Madrid F
[Ad] Endereço:Hospital Universitario de la Princesa, Instituto Investigación Sanitaria Princesa (IIS-IP), Universidad Autónoma de Madrid, Madrid, Spain.
[Ti] Título:CD69: from activation marker to metabolic gatekeeper.
[So] Source:Eur J Immunol;47(6):946-953, 2017 Jun.
[Is] ISSN:1521-4141
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:CD69 is a membrane-bound, type II C-lectin receptor. It is a classical early marker of lymphocyte activation due to its rapid appearance on the surface of the plasma membrane after stimulation. CD69 is expressed by several subsets of tissue resident immune cells, including resident memory T (TRM) cells and gamma delta (γδ) T cells, and is therefore considered a marker of tissue retention. Recent evidence has revealed that CD69 regulates some specific functions of selected T-cell subsets, determining the migration-retention ratio as well as the acquisition of effector or regulatory phenotypes. Specifically, CD69 regulates the differentiation of regulatory T (Treg) cells as well as the secretion of IFN-γ, IL-17, and IL-22. The identification of putative CD69 ligands, such as Galectin-1 (Gal-1), suggests that CD69-induced signaling can be regulated not only during cognate contacts between T cells and antigen-presenting cells in lymphoid organs, but also in the periphery, where cytokines and other metabolites control the final outcome of the immune response. Here, we will discuss new aspects of the molecular signaling mediated by CD69 and its involvement in the metabolic reprogramming regulating TH-effector lineages.
[Mh] Termos MeSH primário: Antígenos CD/metabolismo
Antígenos de Diferenciação de Linfócitos T/metabolismo
Lectinas Tipo C/metabolismo
Ativação Linfocitária
Subpopulações de Linfócitos T/imunologia
[Mh] Termos MeSH secundário: Animais
Células Apresentadoras de Antígenos/imunologia
Células Apresentadoras de Antígenos/fisiologia
Antígenos CD/genética
Antígenos CD/imunologia
Antígenos de Diferenciação de Linfócitos T/genética
Antígenos de Diferenciação de Linfócitos T/imunologia
Diferenciação Celular
Citocinas/imunologia
Citocinas/secreção
Galectinas/imunologia
Regulação da Expressão Gênica
Seres Humanos
Interleucina-17/imunologia
Interleucina-17/secreção
Lectinas Tipo C/genética
Lectinas Tipo C/imunologia
Transdução de Sinais
Subpopulações de Linfócitos T/fisiologia
Linfócitos T Reguladores/imunologia
Linfócitos T Reguladores/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antigens, CD); 0 (Antigens, Differentiation, T-Lymphocyte); 0 (CD69 antigen); 0 (Cytokines); 0 (Galectins); 0 (Interleukin-17); 0 (Lectins, C-Type)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170506
[St] Status:MEDLINE
[do] DOI:10.1002/eji.201646837



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