Base de dados : MEDLINE
Pesquisa : D23.050.327.150 [Categoria DeCS]
Referências encontradas : 57 [refinar]
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[PMID]:25780883
[Au] Autor:Heydari Zarnagh H; Ravanshad M; Pourfatollah AA; Rasaee MJ
[Ad] Endereço:Department of Clinical Biochemistry, Faculity of Medical Science, Tarbiat Modares University, Tehran, Iran Hafez.heydari@modares.ac.ir.
[Ti] Título:Expression and Purification of a Novel Computationally Designed Antigen for Simultaneously Detection of HTLV-1 and HBV Antibodies.
[So] Source:Iran J Allergy Asthma Immunol;14(2):168-78, 2015 Apr.
[Is] ISSN:1735-1502
[Cp] País de publicação:Iran
[La] Idioma:eng
[Ab] Resumo:Computational tools are reliable alternatives to laborious work in chimeric protein design. In this study, a chimeric antigen was designed using computational techniques for simultaneous detection of anti-HTLV-I and anti-HBV in infected sera. Databases were searched for amino acid sequences of HBV/HLV-I diagnostic antigens. The immunodominant fragments were selected based on propensity scales. The diagnostic antigen was designed using these fragments. Secondary and tertiary structures were predicted and the B-cell epitopes were mapped on the surface of built model. The synthetic DNA coding antigen was sub-cloned into pGS21a expression vector. SDS-PAGE analysis showed that glutathione fused antigen was highly expressed in E. coli BL21 (DE3) cells. The recombinant antigen was purified by nickel affinity chromatography. ELISA results showed that soluble antigen could specifically react with the HTLV-I and HBV infected sera. This specific antigen could be used as suitable agent for antibody-antigen based screening tests and can help clinicians in order to perform quick and precise screening of the HBV and HTLV-I infections.
[Mh] Termos MeSH primário: Biologia Computacional/métodos
Anticorpos Antideltaretrovirus/análise
Antígenos de Deltaretrovirus/imunologia
Anticorpos Anti-Hepatite B/análise
Antígenos de Superfície da Hepatite B/imunologia
Proteínas Recombinantes de Fusão/síntese química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Antígenos de Deltaretrovirus/química
Antígenos de Deltaretrovirus/isolamento & purificação
Infecções por Deltaretrovirus/diagnóstico
Ensaio de Imunoadsorção Enzimática
Epitopos de Linfócito B/química
Epitopos de Linfócito B/imunologia
Hepatite B/diagnóstico
Antígenos de Superfície da Hepatite B/química
Seres Humanos
Epitopos Imunodominantes/química
Epitopos Imunodominantes/imunologia
Infecções Tumorais por Vírus/diagnóstico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Deltaretrovirus Antibodies); 0 (Deltaretrovirus Antigens); 0 (Epitopes, B-Lymphocyte); 0 (Hepatitis B Antibodies); 0 (Hepatitis B Surface Antigens); 0 (Immunodominant Epitopes); 0 (Recombinant Fusion Proteins)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:150318
[Lr] Data última revisão:
150318
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150318
[St] Status:MEDLINE


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[PMID]:21994781
[Au] Autor:Abrams A; Akahata Y; Jacobson S
[Ad] Endereço:Neuroimmunology Branch, National Institute of Neurological Diseases and Stroke, National Institutes of Health, Bethesda, MD 20892, USA. abramsan@mail.nih.gov
[Ti] Título:The prevalence and significance of HTLV-I/II seroindeterminate Western blot patterns.
[So] Source:Viruses;3(8):1320-31, 2011 08.
[Is] ISSN:1999-4915
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Human T-lymphotropic virus type I (HTLV-I) infects an estimated 15-20 million persons worldwide. A number of diseases have been associated with the virus including adult T-cell leukemia (ATL), HTLV-associated myelopathy/tropical spastic paraparesis (HAM/TSP), HTLV-I uveitis, and HTLV-I-associated infective dermatitis. Once it was shown that there is an increased risk for developing HAM/TSP associated with blood transfusion, screening for HTLV-1 among blood banks was implemented in Japan, United States, France, and the Netherlands. This process includes detection by an enzyme immunoassay (EIA) followed by a confirmatory Western blot (WB) in which recombinant proteins specific for HTLV-I Env glycoproteins are incorporated into WB strips. HTLV-I seropositive results are defined by the presence of antibodies against either gp46 or gp62/68 (both Env protein bands) and either p19, p24, or p53 (one of the gag bands). HTLV-II seropositivity is confirmed by the presence of rgp46-II. However, numerous cases have been documented in which serum samples are reactive by EIA, but an incomplete banding pattern is displayed by subsequent confirmatory WB. Although the significance of these HTLV-I/II seroindeterminates is unclear, it may suggest a much higher incidence of exposure to HTLV-I/II than previously estimated.
[Mh] Termos MeSH primário: Western Blotting
Infecções por HTLV-I/epidemiologia
Infecções por HTLV-II/epidemiologia
Vírus 1 Linfotrópico T Humano/imunologia
Vírus 2 Linfotrópico T Humano/imunologia
[Mh] Termos MeSH secundário: Formação de Anticorpos
Reações Antígeno-Anticorpo
Antígenos de Deltaretrovirus/imunologia
Produtos do Gene env/imunologia
Anticorpos Anti-HTLV-I/sangue
Infecções por HTLV-I/imunologia
Infecções por HTLV-I/virologia
Anticorpos Anti-HTLV-II/sangue
Infecções por HTLV-II/imunologia
Infecções por HTLV-II/virologia
Seres Humanos
Técnicas Imunoenzimáticas
Paraparesia Espástica Tropical/imunologia
Paraparesia Espástica Tropical/virologia
Prevalência
Proteínas Oncogênicas de Retroviridae/imunologia
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Deltaretrovirus Antigens); 0 (Gene Products, env); 0 (HTLV-I Antibodies); 0 (HTLV-II Antibodies); 0 (Retroviridae Proteins, Oncogenic); 0 (gp46 protein, Human T-cell leukemia virus type I)
[Em] Mês de entrada:1203
[Cu] Atualização por classe:170511
[Lr] Data última revisão:
170511
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:111014
[St] Status:MEDLINE
[do] DOI:10.3390/v3081320


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[PMID]:19279090
[Au] Autor:Jones KS; Huang YK; Chevalier SA; Afonso PV; Petrow-Sadowski C; Bertolette DC; Gessain A; Ruscetti FW; Mahieux R
[Ad] Endereço:Basic Science Program, SAIC-Frederick, National Cancer Institute-Frederick, Frederick, MD 21702-1201, USA. joneska@mail.nih.gov
[Ti] Título:The receptor complex associated with human T-cell lymphotropic virus type 3 (HTLV-3) Env-mediated binding and entry is distinct from, but overlaps with, the receptor complexes of HTLV-1 and HTLV-2.
[So] Source:J Virol;83(10):5244-55, 2009 May.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Little is known about the transmission or tropism of the newly discovered human retrovirus, human T-cell lymphotropic virus type 3 (HTLV-3). Here, we examine the entry requirements of HTLV-3 using independently expressed Env proteins. We observed that HTLV-3 surface glycoprotein (SU) binds efficiently to both activated CD4(+) and CD8(+) T cells. This contrasts with both HTLV-1 SU, which primarily binds to activated CD4(+) T cells, and HTLV-2 SU, which primarily binds to activated CD8(+) T cells. Binding studies with heparan sulfate proteoglycans (HSPGs) and neuropilin-1 (NRP-1), two molecules important for HTLV-1 entry, revealed that these molecules also enhance HTLV-3 SU binding. However, unlike HTLV-1 SU, HTLV-3 SU can bind efficiently in the absence of both HSPGs and NRP-1. Studies of entry performed with HTLV-3 Env-pseudotyped viruses together with SU binding studies revealed that, for HTLV-1, glucose transporter 1 (GLUT-1) functions at a postbinding step during HTLV-3 Env-mediated entry. Further studies revealed that HTLV-3 SU binds efficiently to naive CD4(+) T cells, which do not bind either HTLV-1 or HTLV-2 SU and do not express detectable levels of HSPGs, NRP-1, and GLUT-1. These results indicate that the complex of receptor molecules used by HTLV-3 to bind to primary T lymphocytes differs from that of both HTLV-1 and HTLV-2.
[Mh] Termos MeSH primário: Antígenos de Deltaretrovirus/metabolismo
Deltaretrovirus/metabolismo
Produtos do Gene env/metabolismo
Receptores Virais/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Linfócitos T CD4-Positivos/virologia
Linfócitos T CD8-Positivos/virologia
Linhagem Celular
Deltaretrovirus/genética
Deltaretrovirus/fisiologia
Transportador de Glucose Tipo 1/metabolismo
Proteoglicanas de Heparan Sulfato/metabolismo
Seres Humanos
Dados de Sequência Molecular
Filogenia
Ligação Proteica
Alinhamento de Sequência
Transdução Genética
Ligação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, N.I.H., INTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Deltaretrovirus Antigens); 0 (Gene Products, env); 0 (Glucose Transporter Type 1); 0 (Heparan Sulfate Proteoglycans); 0 (Receptors, Virus)
[Em] Mês de entrada:0904
[Cu] Atualização por classe:161203
[Lr] Data última revisão:
161203
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090313
[St] Status:MEDLINE
[do] DOI:10.1128/JVI.02285-08


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[PMID]:16173022
[Au] Autor:Tsao KC; Chen GW; Huang CG; Huang YL; Lin JY; Mok CK; Keng MC; Sun CF; Shih SR
[Ad] Endereço:Department of Clinical Pathology, Clinical Virology Laboratory, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan.
[Ti] Título:False positive antibody results against human T-cell lymphotropic virus in patients with severe acute respiratory syndrome.
[So] Source:J Med Virol;77(3):331-6, 2005 Nov.
[Is] ISSN:0146-6615
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Taiwan suffered from the outbreak of severe acute respiratory syndrome (SARS) in 2003. Our laboratory performed a series of virology and serology tests for SARS patients admitted to our hospital. Cross-reactivity was found when testing for antibody against human T-cell lymphotropic virus (HTLV) in one patient with SARS. Therefore, antibodies against HTLV were examined in paired-sera from 26 SARS patients. ELISA and a neutralization test were used to measure anti-SARS antibodies. Seroconversion for antibody against SARS-CoV was observed in all patients. Surprisingly, with the use of ELISA for HTLV, sera for 13 patients were positive for HTLV (50%), and seroconversion for HTLV was also observed in 10 patients (38.5%). Western blot for HTLV on those 26 paired-sera from 13 HTLV-positive patients displayed 5 positive results for HTLV-I, 7 positive results for HTLV-II, 1 positive result for both HTLV-I and II, 9 negative results for either HTLV-I or HTLV-II, and 4 "indeterminate" results. The findings that antibody to HTLV can be detected in blood samples collected from SARS patients provide important information for safe handling of blood products. Without such knowledge, blood products can be discarded mistakenly even though they contain anti-SARS-CoV antibodies that may be potentially valuable for SARS therapy.
[Mh] Termos MeSH primário: Anticorpos Antivirais/sangue
Antígenos de Deltaretrovirus/imunologia
Vírus da SARS/imunologia
Síndrome Respiratória Aguda Grave/diagnóstico
Síndrome Respiratória Aguda Grave/imunologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Criança
Reações Cruzadas
Reações Falso-Positivas
Feminino
Infecções por HTLV-I/imunologia
Infecções por HTLV-I/virologia
Infecções por HTLV-II/imunologia
Infecções por HTLV-II/virologia
Seres Humanos
Masculino
Meia-Idade
Testes de Neutralização
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Deltaretrovirus Antigens)
[Em] Mês de entrada:0511
[Cu] Atualização por classe:061115
[Lr] Data última revisão:
061115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:050921
[St] Status:MEDLINE


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[PMID]:15725471
[Au] Autor:Sokol L; Agrawal D; Loughran TP
[Ad] Endereço:Department of Interdisciplinary Oncology, University of South Florida and H Lee, Moffitt Cancer Center and Research Institute, Tampa, FL, USA. sokoll@moffitt.usf.edu
[Ti] Título:Characterization of HTLV envelope seroreactivity in large granular lymphocyte leukemia.
[So] Source:Leuk Res;29(4):381-7, 2005 Apr.
[Is] ISSN:0145-2126
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:T-cell large granular lymphocyte (T-LGL) leukemia is a rare chronic lymphoproliferative disorder of unknown etiology. We have previously reported that patients with T-LGL leukemia were seroreactive against BA21, a 34 amino acid peptide derived from HTLV-I envelope protein p21. We tested sera from 70 patients with T-LGL leukemia and found that 21/70 (30%) of them were seroreactive against fusion peptide GST-BA21. In control group of healthy blood donors 3/30 (10%) were seroreactive. We synthesized a set of overlapping peptides derived from BA21 and tested them against sera from patients. Only a single peptide (p21 env 417-430) showed reactivity. We then generated multiple fusion peptides consisting of 5-14 amino acid residues derived from this peptide and tested them against patient and control sera. Shortest peptide giving positive seroreactivity was octapeptide P8 (p21 env 418-425). Competitive Western blot assay with use of fusion peptides revealed that the minimal HTLV-I epitope responsible for seroreactivity found in patients with T-LGL leukemia is a decapeptide PP10 (p21 env 417-426). Protein Bank (NCBI) search did not reveal any significant homology between PP10 epitope and known human proteins. These results further define the epitope responsible for HTLV env seroreactivity observed in LGL leukemia.
[Mh] Termos MeSH primário: Antígenos de Deltaretrovirus/sangue
Leucemia Linfoide/virologia
Proteínas do Envelope Viral/sangue
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Primers do DNA
Vírus 1 Linfotrópico T Humano/isolamento & purificação
Seres Humanos
Leucemia Linfoide/sangue
Fragmentos de Peptídeos/análise
Fragmentos de Peptídeos/química
Proteínas Recombinantes de Fusão/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Nm] Nome de substância:
0 (DNA Primers); 0 (Deltaretrovirus Antigens); 0 (Peptide Fragments); 0 (Recombinant Fusion Proteins); 0 (Viral Envelope Proteins)
[Em] Mês de entrada:0504
[Cu] Atualização por classe:071115
[Lr] Data última revisão:
071115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:050224
[St] Status:MEDLINE


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[PMID]:14715562
[Au] Autor:De Giuseppe A; Feliziani F; Rutili D; De Mia GM
[Ad] Endereço:Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, 06126 Perugia, Italy.
[Ti] Título:Expression of the bovine leukemia virus envelope glycoprotein (gp51) by recombinant baculovirus and its use in an enzyme-linked immunosorbent assay.
[So] Source:Clin Diagn Lab Immunol;11(1):147-51, 2004 Jan.
[Is] ISSN:1071-412X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The gene encoding the major envelope glycoprotein (gp51) with its signal sequence, represented by an additional NH2-terminal 33-residue amino acid sequence of bovine leukemia virus (BLV), was inserted into a baculovirus transfer vector. A recombinant virus expressing a secreted gp51 protein in insect cells was isolated. The recombinant gp51 expressed was characterized by using an anti-BLV monoclonal antibody by both Western blotting analysis and enzyme-linked immunosorbent assay (ELISA). The secreted gp51 was used as an antigen, and an ELISA with recombinant gp51 (rgp51) was developed for the detection of BLV antibodies. This new procedure was compared with a previous ELISA method for the detection of BLV antibodies and an agar gel immunodiffusion test performed with an unpurified BLV antigen preparation. The comparative testing of field samples showed that the ELISA with rgp51 is more specific and also suitable for the testing of pooled sera.
[Mh] Termos MeSH primário: Ensaio de Imunoadsorção Enzimática/métodos
Vírus da Leucemia Bovina/genética
Vírus da Leucemia Bovina/imunologia
Proteínas do Envelope Viral/genética
Proteínas do Envelope Viral/imunologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Anticorpos Monoclonais
Baculoviridae/genética
Western Blotting
Bovinos
Doenças dos Bovinos/diagnóstico
Doenças dos Bovinos/imunologia
Linhagem Celular
Clonagem Molecular
Anticorpos Antideltaretrovirus/análise
Antígenos de Deltaretrovirus/genética
Leucose Enzoótica Bovina/diagnóstico
Leucose Enzoótica Bovina/imunologia
Genes Virais
Vetores Genéticos
Dados de Sequência Molecular
Proteínas Recombinantes/genética
Proteínas Recombinantes/imunologia
Homologia de Sequência de Aminoácidos
Ovinos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Deltaretrovirus Antibodies); 0 (Deltaretrovirus Antigens); 0 (Recombinant Proteins); 0 (Viral Envelope Proteins)
[Em] Mês de entrada:0404
[Cu] Atualização por classe:140610
[Lr] Data última revisão:
140610
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:040113
[St] Status:MEDLINE


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[PMID]:14622599
[Au] Autor:Manel N; Kim FJ; Kinet S; Taylor N; Sitbon M; Battini JL
[Ad] Endereço:Institut de Génétique Moléculaire de Montpellier, CNRS UMR 5535/IFR 122, F-34293 Montpellier Cedex 5, France.
[Ti] Título:The ubiquitous glucose transporter GLUT-1 is a receptor for HTLV.
[So] Source:Cell;115(4):449-59, 2003 Nov 14.
[Is] ISSN:0092-8674
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The human T cell leukemia virus (HTLV) is associated with leukemia and neurological syndromes. The physiopathological effects of HTLV envelopes are unclear and the identity of the receptor, present on all vertebrate cell lines, has been elusive. We show that the receptor binding domains of both HTLV-1 and -2 envelope glycoproteins inhibit glucose transport by interacting with GLUT-1, the ubiquitous vertebrate glucose transporter. Receptor binding and HTLV envelope-driven infection are selectively inhibited when glucose transport or GLUT-1 expression are blocked by cytochalasin B or siRNAs, respectively. Furthermore, ectopic expression of GLUT-1, but not the related transporter GLUT-3, restores HTLV infection abrogated by either GLUT-1 siRNAs or interfering HTLV envelope glycoproteins. Therefore, GLUT-1 is a receptor for HTLV. Perturbations in glucose metabolism resulting from interactions of HTLV envelope glycoproteins with GLUT-1 are likely to contribute to HTLV-associated disorders.
[Mh] Termos MeSH primário: Vírus 1 Linfotrópico T Humano/metabolismo
Vírus 2 Linfotrópico T Humano/metabolismo
Proteínas de Transporte de Monossacarídeos/metabolismo
Receptores Virais/metabolismo
[Mh] Termos MeSH secundário: Animais
Transporte Biológico/efeitos dos fármacos
Linhagem Celular
Citocalasina B/farmacologia
Antígenos de Deltaretrovirus/metabolismo
Glucose/antagonistas & inibidores
Glucose/metabolismo
Transportador de Glucose Tipo 1
Células HeLa
Vírus 1 Linfotrópico T Humano/fisiologia
Vírus 2 Linfotrópico T Humano/fisiologia
Seres Humanos
Ácido Láctico/metabolismo
Camundongos
Proteínas de Transporte de Monossacarídeos/genética
Ligação Proteica/efeitos dos fármacos
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
RNA Interferente Pequeno/genética
RNA Interferente Pequeno/metabolismo
Receptores Virais/genética
Proteínas do Envelope Viral/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Deltaretrovirus Antigens); 0 (Glucose Transporter Type 1); 0 (Monosaccharide Transport Proteins); 0 (RNA, Messenger); 0 (RNA, Small Interfering); 0 (Receptors, Virus); 0 (SLC2A1 protein, human); 0 (Slc2a1 protein, mouse); 0 (Viral Envelope Proteins); 33X04XA5AT (Lactic Acid); 3CHI920QS7 (Cytochalasin B); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:0401
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:031119
[St] Status:MEDLINE


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[PMID]:14615889
[Au] Autor:Stark P; Bodemer W; Hannig H; Luboshitz J; Shaklai M; Shohat B
[Ad] Endereço:Institute of Hematology, Rabin Medical Center, Beilinson Campus, 49100 Petah Tiqva, Israel. starkp@post.tau.ac.il
[Ti] Título:Human T lymphotropic virus type 1 in a seronegative B chronic lymphocytic leukemia patient.
[So] Source:Med Microbiol Immunol;192(4):205-9, 2003 Nov.
[Is] ISSN:0300-8584
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Human T lymphotropic virus type 1 (HTLV-1) is the etiological agent of adult T cell leukemia and HTLV-1-associated myelopathy/tropical spastic paraparesis. HTLV-1 infection in patients with B cell-type chronic lymphocytic leukemia (B-CLL) is rare and has been reported only in areas in which HTLV-1 is endemic. In the present study, we detected HTLV-1 proviral DNA by polymerase chain reaction, using tax primers, in peripheral blood lymphocytes from a B-CLL patient, an immigrant to Israel, where HTLV-1 infection is not endemic. F344 rats injected intravenously with peripheral blood lymphocytes obtained from the patient developed HTLV-1 antibodies. Titers of antibody to HTLV-1 in the rat blood were 1:512 by particle agglutination; enzyme-linked immunosorbent assay and Western blotting were also positive. No antibody against HTLV-1 was demonstrated in the animal model after inoculation of either purified B lymphocytes from the B-CLL patient or peripheral blood mononuclear cells from healthy donors. This is one of the few studies showing the presence of HTLV-1 provirus in T lymphocytes of a B-CLL patient who had multiple infections, and died of salmonella sepsis, and the first report of HTLV-1 antibody induction in an animal model by inoculation of lymphocytes obtained from an HTLV-1-infected B-CLL patient.
[Mh] Termos MeSH primário: Infecções por HTLV-I/virologia
Vírus 1 Linfotrópico T Humano/isolamento & purificação
Leucemia Linfocítica Crônica de Células B/virologia
Linfócitos T/virologia
[Mh] Termos MeSH secundário: Idoso
Testes de Aglutinação
Animais
Western Blotting
Anticorpos Antideltaretrovirus/sangue
Antígenos de Deltaretrovirus/imunologia
Modelos Animais de Doenças
Ensaio de Imunoadsorção Enzimática
Evolução Fatal
Feminino
Infecções por HTLV-I/transmissão
Vírus 1 Linfotrópico T Humano/genética
Vírus 1 Linfotrópico T Humano/imunologia
Seres Humanos
Israel
Leucemia Linfocítica Crônica de Células B/complicações
Reação em Cadeia da Polimerase
Ratos
Ratos Endogâmicos F344
Sepse
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Deltaretrovirus Antibodies); 0 (Deltaretrovirus Antigens)
[Em] Mês de entrada:0403
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:031115
[St] Status:MEDLINE


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[PMID]:12239314
[Au] Autor:Ding W; Albrecht B; Kelley RE; Muthusamy N; Kim SJ; Altschuld RA; Lairmore MD
[Ad] Endereço:Center for Retrovirus Research, Department of Veterinary Biosciences, The Ohio State University, 1925 Coffey Road, Columbus, OH 43210, USA.
[Ti] Título:Human T-cell lymphotropic virus type 1 p12(I) expression increases cytoplasmic calcium to enhance the activation of nuclear factor of activated T cells.
[So] Source:J Virol;76(20):10374-82, 2002 Oct.
[Is] ISSN:0022-538X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Human T-cell lymphotropic virus type 1 (HTLV-1) establishes persistent infection and is associated with lymphoproliferative or neurodegenerative diseases. As a complex retrovirus, HTLV-1 contains typical structural and enzymatic genes, as well as regulatory and accessory genes encoded in the pX region. The early events necessary for HTLV-1 to establish infection in lymphocytes, its primary target cells, remain unresolved. Recent studies have demonstrated the importance of regulatory and accessory gene products in determining this virus-host interaction. Among these, pX open reading frame I, which encodes two proteins, p12(I) and p27(I), is required for establishing persistent infection in vivo and for infection in quiescent primary lymphocytes. In addition, p12(I) localizes in the endoplasmic reticulum (ER) and cis-Golgi apparatus and associates with a calcium binding protein, calreticulin. We recently reported that p12(I) expression induces the calcium-responsive T-cell transcription factor, nuclear factor of activated T cells (NFAT), in the presence of phorbol ester activation. Based on these studies, we hypothesize that p12(I) may modulate calcium release from the ER. Here, we report that p12(I) expression increases basal cytoplasmic calcium and concurrently diminishes calcium available for release from the ER stores. Overexpression of calreticulin, a calcium buffer protein, blocked p12(I)-mediated NFAT activation independently of its ability to bind p12(I). Chemical inhibition studies using inhibitors of inositol 1,4,5-triphosphate receptor and calcium release-activated calcium channels suggest that inositol 1,4,5-triphosphate receptor in the ER membrane and calcium release-activated calcium channels in the plasma membrane contribute to p12(I)-mediated NFAT activation. Collectively, our results are the first to demonstrate the role of p12(I) in elevating cytoplasmic calcium, an antecedent to T-cell activation, and further support the important role of this accessory protein in the early events of HTLV-1 infection.
[Mh] Termos MeSH primário: Proteínas de Ligação a DNA/metabolismo
Antígenos de Deltaretrovirus/biossíntese
Vírus 1 Linfotrópico T Humano/metabolismo
Proteínas Nucleares
Proteínas Oncogênicas Virais/biossíntese
Linfócitos T/metabolismo
Fatores de Transcrição/metabolismo
[Mh] Termos MeSH secundário: Cálcio/metabolismo
Canais de Cálcio/metabolismo
Proteínas de Ligação ao Cálcio/metabolismo
Proteínas de Ligação ao Cálcio/farmacologia
Calreticulina
Citoplasma/metabolismo
Antígenos de Deltaretrovirus/genética
Retículo Endoplasmático/metabolismo
Expressão Gênica
Células HeLa
Seres Humanos
Receptores de Inositol 1,4,5-Trifosfato
Células Jurkat
Ativação Linfocitária
Fatores de Transcrição NFATC
Proteínas Oncogênicas Virais/genética
Receptores Citoplasmáticos e Nucleares/metabolismo
Ribonucleoproteínas/metabolismo
Ribonucleoproteínas/farmacologia
Proteínas Virais Reguladoras e Acessórias
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Nm] Nome de substância:
0 (Calcium Channels); 0 (Calcium-Binding Proteins); 0 (Calreticulin); 0 (DNA-Binding Proteins); 0 (Deltaretrovirus Antigens); 0 (ITPR1 protein, human); 0 (Inositol 1,4,5-Trisphosphate Receptors); 0 (NFATC Transcription Factors); 0 (Nuclear Proteins); 0 (Oncogene Proteins, Viral); 0 (Receptors, Cytoplasmic and Nuclear); 0 (Ribonucleoproteins); 0 (Transcription Factors); 0 (Viral Regulatory and Accessory Proteins); 0 (p12I protein, Human T-lymphotropic virus 1); SY7Q814VUP (Calcium)
[Em] Mês de entrada:0210
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:020920
[St] Status:MEDLINE


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[PMID]:11595747
[Au] Autor:Wilson KA; Maerz AL; Poumbourios P
[Ad] Endereço:St. Vincent's Institute of Medical Research, Fitzroy, Victoria 3065 Australia.
[Ti] Título:Evidence that the transmembrane domain proximal region of the human T-cell leukemia virus type 1 fusion glycoprotein gp21 has distinct roles in the prefusion and fusion-activated states.
[So] Source:J Biol Chem;276(52):49466-75, 2001 Dec 28.
[Is] ISSN:0021-9258
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To investigate the structural context of the fusion peptide region in human T-cell leukemia virus type 1 gp21, maltose-binding protein (MBP) was used as an N-terminal solubilization partner for the entire gp21 ectodomain (residues 313-445) and C-terminally truncated ectodomain fragments. The bacterial expression of the MBP/gp21 chimeras resulted in soluble trimers containing intramonomer disulfide bonds. Detergents blocked the proteolytic cleavage of fusion peptide residues in the MBP/gp21-(313-425) chimera, indicating that the fusion peptide is available for interaction with detergent despite the presence of an N-terminal MBP domain. Limited proteolysis experiments indicated that the transmembrane domain proximal sequence Thr(425)-Ala(439) protects fusion peptide residues from chymotrypsin. MBP/gp21 chimera stability therefore depends on a functional interaction between N-terminal and transmembrane domain proximal regions in a gp21 helical hairpin structure. In addition, thermal aggregation experiments indicated that the Thr(425)-Ser(436) sequence confers stability to the fusion peptide-containing MBP/gp21 chimeras. The functional role of the transmembrane domain proximal sequence was assessed by alanine-scanning mutagenesis of the full-length envelope glycoprotein, with 11 of 12 single alanine substitutions resulting in 1.5- to 4.5-fold enhancements in cell-cell fusion activity. By contrast, single alanine substitutions in MBP/gp21 did not significantly alter chimera stability, indicating that multiple residues within the transmembrane domain proximal region and the fusion peptide and adjacent glycine-rich segment contribute to stability, thereby mitigating the potential effects of the substitutions. The fusion-enhancing effects of the substitutions are therefore likely to be caused by alteration of the prefusion complex. Our observations suggest that the function of the transmembrane domain proximal sequence in the prefusion envelope glycoprotein is distinct from its role in stabilizing the fusion peptide region in the fusion-activated helical hairpin conformation of gp21.
[Mh] Termos MeSH primário: Antígenos de Deltaretrovirus/metabolismo
Produtos do Gene env/metabolismo
Vírus 1 Linfotrópico T Humano/fisiologia
Proteínas Oncogênicas de Retroviridae/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Proteínas de Transporte/química
Proteínas de Transporte/genética
Proteínas de Transporte/metabolismo
Cromatografia em Gel
Quimotripsina/metabolismo
Antígenos de Deltaretrovirus/genética
Detergentes/química
Dissulfetos/química
Produtos do Gene env/química
Produtos do Gene env/genética
Vírus 1 Linfotrópico T Humano/genética
Seres Humanos
Proteínas Ligantes de Maltose
Dados de Sequência Molecular
Estrutura Secundária de Proteína
Estrutura Terciária de Proteína
Proteínas Recombinantes de Fusão/química
Proteínas Recombinantes de Fusão/metabolismo
Proteínas Oncogênicas de Retroviridae/química
Proteínas Oncogênicas de Retroviridae/genética
Temperatura Ambiente
Produtos do Gene env do Vírus da Imunodeficiência Humana
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (Deltaretrovirus Antigens); 0 (Detergents); 0 (Disulfides); 0 (Gene Products, env); 0 (Maltose-Binding Proteins); 0 (Recombinant Fusion Proteins); 0 (Retroviridae Proteins, Oncogenic); 0 (env Gene Products, Human Immunodeficiency Virus); 0 (gp21 protein, Human T-lymphotropic virus 1); EC 3.4.21.1 (Chymotrypsin)
[Em] Mês de entrada:0201
[Cu] Atualização por classe:101118
[Lr] Data última revisão:
101118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:011012
[St] Status:MEDLINE



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