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[PMID]: | 28582663 |
[Au] Autor: | Umeda K; Nakamura H; Yamamoto K; Nishina N; Yasufuku K; Hirai Y; Hirayama T; Goto K; Hase A; Ogasawara J |
[Ad] Endereço: | Division of Microbiology, Osaka Institute of Public Health, 8-34, Tojo-cho, Tennoji-ku, Osaka 543-0026, Japan. Electronic address: kaor-umeda@iph.osaka.jp. |
[Ti] Título: | Molecular and epidemiological characterization of staphylococcal foodborne outbreak of Staphylococcus aureus harboring seg, sei, sem, sen, seo, and selu genes without production of classical enterotoxins. |
[So] Source: | Int J Food Microbiol;256:30-35, 2017 Sep 01. | [Is] ISSN: | 1879-3460 |
[Cp] País de publicação: | Netherlands |
[La] Idioma: | eng |
[Ab] Resumo: | Staphylococcal food poisoning is the result of consumption of food contaminated with staphylococcal enterotoxins (SEs) produced by Staphylococcus aureus. To date, 23 SEs and SE-like enterotoxins (SEls) have been described in the literature. They are divided into classical SEs (SEA-SEE) and new SE/SEls (SEG-SElX). Some have proved to be foodborne-inducible, but others remain unidentified. In May 2016, at an elderly group home in Osaka city, Japan, an outbreak from foodborne pathogens occurred among lunch party participants. Within 2h 30min to 4h 40min, 15 of 53 participants presented gastrointestinal symptoms of vomiting, diarrhea, and nausea. A subsequent laboratory investigation detected S. aureus from most stool samples from patients, several left-over food items, a kitchen swab, and hand swabs from two food handlers. Classical SEs was not detected from S. aureus isolates or left-over food items. From examination for the presence of SE/SEl genes of 20 kinds by PCR, seg, sei, sem, sen, seo, and selu genes were detected in almost all isolates. These isolates exhibited identical or closely related types by coagulase type (type VII), Sma I digested pulsed-field gel electrophoresis analysis and multi-locus sequence typing (MLST-CC45 lineage). These results suggest that the foodborne outbreak was caused by S. aureus harboring seg, sei, sem, sen, seo, and selu genes without production of classical SEs. Additionally, some S. aureus isolates from human nasal swabs and healthy human feces harboring seg, sei, sem, sen, seo, and selu genes without production of classical SEs were classified into CC45 lineage using MLST. These findings suggest new SE/SEls as a potential cause of foodborne outbreaks. |
[Mh] Termos MeSH primário: |
Surtos de Doenças Enterotoxinas/genética Intoxicação Alimentar Estafilocócica/epidemiologia Staphylococcus aureus/genética Staphylococcus aureus/isolamento & purificação
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[Mh] Termos MeSH secundário: |
Primers do DNA Eletroforese em Gel de Campo Pulsado Enterotoxinas/biossíntese Seres Humanos Japão/epidemiologia Tipagem de Sequências Multilocus Reação em Cadeia da Polimerase/métodos Intoxicação Alimentar Estafilocócica/microbiologia Infecções Estafilocócicas/microbiologia Superantígenos/genética
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (DNA Primers); 0 (Enterotoxins); 0 (Superantigens); 0 (enterotoxin G, staphylococcal); 0 (enterotoxin I, staphylococcal) |
[Em] Mês de entrada: | 1710 |
[Cu] Atualização por classe: | 171031 |
[Lr] Data última revisão:
| 171031 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170606 |
[St] Status: | MEDLINE |
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