Base de dados : MEDLINE
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[PMID]:29199992
[Au] Autor:Oulavallickal T; Brewster JL; McKellar JLO; Fairhurst MJ; Tenci NA; Gerth ML
[Ad] Endereço:Department of Biochemistry, University of Otago, PO Box 56, Dunedin, Otago 9054, New Zealand.
[Ti] Título:The Pseudomonas syringae pv. actinidiae chemoreceptor protein F (PscF) periplasmic sensor domain: cloning, purification and X-ray crystallographic analysis.
[So] Source:Acta Crystallogr F Struct Biol Commun;73(Pt 12):701-705, 2017 Dec 01.
[Is] ISSN:2053-230X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Nitrate- and nitrite-sensing (NIT) domains are found associated with a wide variety of bacterial receptors, including chemoreceptors. However, the structure of a chemoreceptor-associated NIT domain has not yet been characterized. Recently, a chemoreceptor named PscF was identified from the plant pathogen Pseudomonas syringae pv. actinidiae that is predicted to contain a periplasmic NIT domain. The PscF sensor domain (PscF-SD; residues 42-332) was cloned into an appropriate expression vector, recombinantly produced in Escherichia coli BL21-Gold(DE3) cells and purified via immobilized metal-affinity and size-exclusion chromatography. Purified PscF-SD was screened for crystallization; the best crystal diffracted to a maximum resolution of 1.46 Šin space group P2 2 2 . However, the data could not be phased using the only available NIT-domain structure (Klebsiella oxytoca NasR; PDB entry 4akk) as the search model. Therefore, a data set from a selenomethionine-labelled protein crystal was also collected. The selenomethionine-labelled protein crystal diffracted to a resolution of 2.46 Šin space group P2 2 2 . These data will be used to attempt to solve the structure using the single-wavelength anomalous diffraction technique. The structure is expected to provide insights into the ligand specificity of NIT domains and the role of NIT domains in chemotaxis.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Pseudomonas syringae/química
[Mh] Termos MeSH secundário: Proteínas de Bactérias/isolamento & purificação
Fatores Quimiotáticos
Cromatografia de Afinidade
Cromatografia em Gel
Clonagem Molecular
Cristalografia por Raios X
Nitratos/química
Nitratos/metabolismo
Nitritos/química
Nitritos/metabolismo
Periplasma/metabolismo
Domínios Proteicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Chemotactic Factors); 0 (Nitrates); 0 (Nitrites)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE
[do] DOI:10.1107/S2053230X17016831


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[PMID]:28893946
[Au] Autor:Pauerstein PT; Tellez K; Willmarth KB; Park KM; Hsueh B; Efsun Arda H; Gu X; Aghajanian H; Deisseroth K; Epstein JA; Kim SK
[Ad] Endereço:Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
[Ti] Título:A radial axis defined by semaphorin-to-neuropilin signaling controls pancreatic islet morphogenesis.
[So] Source:Development;144(20):3744-3754, 2017 10 15.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The islets of Langerhans are endocrine organs characteristically dispersed throughout the pancreas. During development, endocrine progenitors delaminate, migrate radially and cluster to form islets. Despite the distinctive distribution of islets, spatially localized signals that control islet morphogenesis have not been discovered. Here, we identify a radial signaling axis that instructs developing islet cells to disperse throughout the pancreas. A screen of pancreatic extracellular signals identified factors that stimulated islet cell development. These included semaphorin 3a, a guidance cue in neural development without known functions in the pancreas. In the fetal pancreas, peripheral mesenchymal cells expressed Sema3a, while central nascent islet cells produced the semaphorin receptor neuropilin 2 (Nrp2). Nrp2 mutant islet cells developed in proper numbers, but had defects in migration and were unresponsive to purified Sema3a. Mutant Nrp2 islets aggregated centrally and failed to disperse radially. Thus, Sema3a-Nrp2 signaling along an unrecognized pancreatic developmental axis constitutes a chemoattractant system essential for generating the hallmark morphogenetic properties of pancreatic islets. Unexpectedly, Sema3a- and Nrp2-mediated control of islet morphogenesis is strikingly homologous to mechanisms that regulate radial neuronal migration and cortical lamination in the developing mammalian brain.
[Mh] Termos MeSH primário: Ilhotas Pancreáticas/citologia
Neuropilina-2/metabolismo
Semaforina-3A/metabolismo
[Mh] Termos MeSH secundário: Animais
Adesão Celular
Movimento Celular
Fatores Quimiotáticos/química
Regulação da Expressão Gênica no Desenvolvimento
Seres Humanos
Ligantes
Camundongos
Camundongos Knockout
Morfogênese
Mutação
Neurônios/metabolismo
Neuropilina-2/genética
Pâncreas/citologia
Semaforina-3A/genética
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chemotactic Factors); 0 (Ligands); 0 (Neuropilin-2); 0 (SEMA3A protein, human); 0 (Sema3a protein, mouse); 0 (Semaphorin-3A)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE
[do] DOI:10.1242/dev.148684


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[PMID]:28808157
[Au] Autor:Bhowmick R; Clark S; Bonventre JV; Leong JM; McCormick BA
[Ad] Endereço:School of Chemical Engineering, Oklahoma State University, Stillwater, Oklahoma, USA.
[Ti] Título:Cytosolic Phospholipase A α Promotes Pulmonary Inflammation and Systemic Disease during Streptococcus pneumoniae Infection.
[So] Source:Infect Immun;85(11), 2017 Nov.
[Is] ISSN:1098-5522
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pulmonary infection by is characterized by a robust alveolar infiltration of neutrophils (polymorphonuclear cells [PMNs]) that can promote systemic spread of the infection if not resolved. We previously showed that 12-lipoxygenase (12-LOX), which is required to generate the PMN chemoattractant hepoxilin A (HXA ) from arachidonic acid (AA), promotes acute pulmonary inflammation and systemic infection after lung challenge with As phospholipase A (PLA ) promotes the release of AA, we investigated the role of PLA in local and systemic disease during infection. The group IVA cytosolic isoform of PLA (cPLA α) was activated upon infection of cultured lung epithelial cells and was critical for AA release from membrane phospholipids. Pharmacological inhibition of this enzyme blocked -induced PMN transepithelial migration Genetic ablation of the cPLA isoform cPLA α dramatically reduced lung inflammation in mice upon high-dose pulmonary challenge with The cPLA α-deficient mice also suffered no bacteremia and survived a pulmonary challenge that was lethal to wild-type mice. Our data suggest that cPLA α plays a crucial role in eliciting pulmonary inflammation during pneumococcal infection and is required for lethal systemic infection following lung challenge.
[Mh] Termos MeSH primário: Células Epiteliais/imunologia
Fosfolipases A2 do Grupo IV/imunologia
Interações Hospedeiro-Patógeno
Pulmão/imunologia
Infecções Pneumocócicas/imunologia
Pneumonia Bacteriana/imunologia
[Mh] Termos MeSH secundário: Animais
Ácido Araquidônico/imunologia
Ácido Araquidônico/metabolismo
Bacteriemia/genética
Bacteriemia/imunologia
Bacteriemia/prevenção & controle
Linhagem Celular Tumoral
Fatores Quimiotáticos/imunologia
Fatores Quimiotáticos/metabolismo
Clorobenzoatos/farmacologia
Cinamatos/farmacologia
Cicloexanonas/farmacologia
Inibidores Enzimáticos/farmacologia
Células Epiteliais/efeitos dos fármacos
Células Epiteliais/enzimologia
Células Epiteliais/microbiologia
Fosfolipases A2 do Grupo IV/antagonistas & inibidores
Fosfolipases A2 do Grupo IV/deficiência
Fosfolipases A2 do Grupo IV/genética
Seres Humanos
Pulmão/efeitos dos fármacos
Pulmão/enzimologia
Pulmão/microbiologia
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Knockout
Infiltração de Neutrófilos/efeitos dos fármacos
Neutrófilos/efeitos dos fármacos
Neutrófilos/imunologia
Neutrófilos/microbiologia
Infecções Pneumocócicas/genética
Infecções Pneumocócicas/microbiologia
Infecções Pneumocócicas/mortalidade
Pneumonia Bacteriana/genética
Pneumonia Bacteriana/microbiologia
Pneumonia Bacteriana/mortalidade
Streptococcus pneumoniae/efeitos dos fármacos
Streptococcus pneumoniae/genética
Streptococcus pneumoniae/patogenicidade
Análise de Sobrevida
Migração Transendotelial e Transepitelial/efeitos dos fármacos
Migração Transendotelial e Transepitelial/imunologia
ortoaminobenzoatos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chemotactic Factors); 0 (Chlorobenzoates); 0 (Cinnamates); 0 (Cyclohexanones); 0 (Enzyme Inhibitors); 0 (ortho-Aminobenzoates); 110683-10-8 (4-amylcinnamoylanthranilic acid); 27YG812J1I (Arachidonic Acid); 83654-05-1 (1,6-bis(cyclohexyloximinocarbonyl)hexane); 99754-06-0 (2-(4-amylcinnamoyl)amino-4-chlorobenzoic acid); EC 3.1.1.4 (Group IV Phospholipases A2)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170816
[St] Status:MEDLINE


  4 / 5553 MEDLINE  
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[PMID]:28593809
[Au] Autor:Hosoi A; Katsuyama T; Sasaki Y; Kondo T; Yajima S; Ito S
[Ad] Endereço:a Department of Bioscience , Tokyo University of Agriculture , Tokyo , Japan.
[Ti] Título:Nitrate analogs as attractants for soybean cyst nematode.
[So] Source:Biosci Biotechnol Biochem;81(8):1542-1547, 2017 Aug.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Soybean cyst nematode (SCN) Heterodera glycines Ichinohe, a plant parasite, is one of the most serious pests of soybean. In this paper, we report that SCN is attracted to nitrate and its analogs. We performed attraction assays to screen for novel attractants for SCN and found that nitrates were attractants for SCN and SCN recognized nitrate gradients. However, attraction of SCN to nitrates was not observed on agar containing nitrate. To further elucidate the attraction mechanism in SCN, we performed attraction assays using nitrate analogs ([Formula: see text], [Formula: see text], [Formula: see text]). SCN was attracted to all nitrate analogs; however, attraction of SCN to nitrate analogs was not observed on agar containing nitrate. In contrast, SCN was attracted to azuki root, irrespective of presence or absence of nitrate in agar media. Our results suggest that the attraction mechanisms differ between plant-derived attractant and nitrate.
[Mh] Termos MeSH primário: Fatores Quimiotáticos/farmacologia
Nitratos/farmacologia
Tylenchoidea/efeitos dos fármacos
[Mh] Termos MeSH secundário: Ágar/farmacologia
Animais
Fatores Quimiotáticos/química
Nitratos/química
Doenças das Plantas/parasitologia
Doenças das Plantas/prevenção & controle
Raízes de Plantas/efeitos dos fármacos
Raízes de Plantas/parasitologia
Feijão de Soja/efeitos dos fármacos
Feijão de Soja/parasitologia
Relação Estrutura-Atividade
Tylenchoidea/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chemotactic Factors); 0 (Nitrates); 9002-18-0 (Agar)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170609
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2017.1332980


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[PMID]:28476332
[Au] Autor:Chourey S; Ye Q; Reddy CN; Cossette C; Gravel S; Zeller M; Slobodchikova I; Vuckovic D; Rokach J; Powell WS
[Ad] Endereço:Claude Pepper Institute and Department of Chemistry, Florida Institute of Technology, 150 West University Boulevard, Melbourne, FL 32901-6982, USA.
[Ti] Título:In vivo α-hydroxylation of a 2-alkylindole antagonist of the OXE receptor for the eosinophil chemoattractant 5-oxo-6,8,11,14-eicosatetraenoic acid in monkeys.
[So] Source:Biochem Pharmacol;138:107-118, 2017 Aug 15.
[Is] ISSN:1873-2968
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We have developed a selective indole antagonist (230) targeting the OXE receptor for the potent eosinophil chemoattractant 5-oxo-ETE (5-oxo-6,8,11,14-eicosatetraenoic acid), that may be useful for the treatment of eosinophilic diseases such as asthma. In previous studies we identified ω2-oxidation of the hexyl side chain of racemic 230 as a major metabolic route in monkeys, but also obtained evidence for another pathway that appeared to involve hydroxylation of the hexyl side chain close to the indole. The present study was designed to investigate the metabolism of the active S-enantiomer of 230 (S230) and to identify the novel hydroxy metabolite and its chirality. Following oral administration, S230 rapidly appeared in the blood along with metabolites formed by a novel and highly stereospecific α-hydroxylation pathway, resulting in the formation of αS-hydroxy-S230. The chirality of α-hydroxy-S230 was determined by the total synthesis of the relevant diastereomers. Of the four possible diastereomers of α-hydroxy-230 only αS-hydroxy-S230 has significant OXE receptor antagonist activity and only this diastereomer was found in significant amounts in blood following oral administration of S230. Other novel metabolites of S230 identified in plasma by LC-MS/MS were αS,ω2-dihydroxy-S230 and glucuronides of S230 and ω2-hydroxy-S230. Thus the alkyl side chain of S230, which is essential for its antagonist activity, is also the major target of the metabolic enzymes that terminate its antagonist activity. Modification of this side chain might result in the development of related antagonists with improved metabolic stability and efficacy.
[Mh] Termos MeSH primário: Antiasmáticos/farmacocinética
Anti-Inflamatórios não Esteroides/farmacocinética
Ácidos Araquidônicos/antagonistas & inibidores
Fatores Quimiotáticos/antagonistas & inibidores
Indóis/farmacocinética
Cetoácidos/farmacocinética
Receptores Eicosanoides/antagonistas & inibidores
[Mh] Termos MeSH secundário: Administração Oral
Alquilação
Animais
Antiasmáticos/administração & dosagem
Antiasmáticos/sangue
Antiasmáticos/farmacologia
Anti-Inflamatórios não Esteroides/administração & dosagem
Anti-Inflamatórios não Esteroides/sangue
Anti-Inflamatórios não Esteroides/farmacologia
Ácidos Araquidônicos/metabolismo
Fatores Quimiotáticos/metabolismo
Eosinófilos/efeitos dos fármacos
Eosinófilos/imunologia
Eosinófilos/metabolismo
Feminino
Glucuronídeos/sangue
Glucuronídeos/química
Glucuronídeos/farmacologia
Seres Humanos
Hidroxilação
Inativação Metabólica
Indóis/administração & dosagem
Indóis/sangue
Indóis/química
Indóis/farmacologia
Cetoácidos/administração & dosagem
Cetoácidos/sangue
Cetoácidos/química
Cetoácidos/farmacologia
Macaca fascicularis
Estrutura Molecular
Neutrófilos/efeitos dos fármacos
Neutrófilos/imunologia
Neutrófilos/metabolismo
Receptores Eicosanoides/agonistas
Receptores Eicosanoides/metabolismo
Estereoisomerismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (5-(5-chloro-2-(1-hydroxyhexyl)-1-methyl-1H-indol-3-yl)-3-methyl-5-oxopentanoate); 0 (5-(5-chloro-2-hexyl-1-methyl-1H-indol-3-yl)-3-methyl-5-oxopentanoate); 0 (Anti-Asthmatic Agents); 0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Arachidonic Acids); 0 (Chemotactic Factors); 0 (Glucuronides); 0 (Indoles); 0 (Keto Acids); 0 (Receptors, Eicosanoid); 126432-17-5 (5-oxo-6,8,11,14-eicosatetraenoic acid)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170507
[St] Status:MEDLINE


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[PMID]:28434801
[Au] Autor:Varadarajan SG; Kong JH; Phan KD; Kao TJ; Panaitof SC; Cardin J; Eltzschig H; Kania A; Novitch BG; Butler SJ
[Ad] Endereço:Department of Neurobiology, University of California, Los Angeles, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USA; Neuroscience Interdisciplinary Graduate Program, University o
[Ti] Título:Netrin1 Produced by Neural Progenitors, Not Floor Plate Cells, Is Required for Axon Guidance in the Spinal Cord.
[So] Source:Neuron;94(4):790-799.e3, 2017 May 17.
[Is] ISSN:1097-4199
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Netrin1 has been proposed to act from the floor plate (FP) as a long-range diffusible chemoattractant for commissural axons in the embryonic spinal cord. However, netrin1 mRNA and protein are also present in neural progenitors within the ventricular zone (VZ), raising the question of which source of netrin1 promotes ventrally directed axon growth. Here, we use genetic approaches in mice to selectively remove netrin from different regions of the spinal cord. Our analyses show that the FP is not the source of netrin1 directing axons to the ventral midline, while local VZ-supplied netrin1 is required for this step. Furthermore, rather than being present in a gradient, netrin1 protein accumulates on the pial surface adjacent to the path of commissural axon extension. Thus, netrin1 does not act as a long-range secreted chemoattractant for commissural spinal axons but instead promotes ventrally directed axon outgrowth by haptotaxis, i.e., directed growth along an adhesive surface.
[Mh] Termos MeSH primário: Orientação de Axônios/genética
Axônios/metabolismo
Fatores de Crescimento Neural/genética
Células-Tronco Neurais/metabolismo
Medula Espinal/embriologia
Proteínas Supressoras de Tumor/genética
[Mh] Termos MeSH secundário: Animais
Axônios/ultraestrutura
Fatores Quimiotáticos/genética
Fatores Quimiotáticos/metabolismo
Imagem Tridimensional
Imuno-Histoquímica
Hibridização In Situ
Camundongos
Camundongos Knockout
Microscopia Confocal
Fatores de Crescimento Neural/metabolismo
Netrina-1
Neurogênese/genética
RNA Mensageiro/metabolismo
Medula Espinal/ultraestrutura
Proteínas Supressoras de Tumor/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chemotactic Factors); 0 (Nerve Growth Factors); 0 (RNA, Messenger); 0 (Tumor Suppressor Proteins); 158651-98-0 (Netrin-1)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170425
[St] Status:MEDLINE


  7 / 5553 MEDLINE  
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[PMID]:28367571
[Au] Autor:Yang K; Wu J; Xu G; Xie D; Peretz-Soroka H; Santos S; Alexander M; Zhu L; Zhang M; Liu Y; Lin F
[Ad] Endereço:Institute of Applied Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, P. R. China.
[Ti] Título:A dual-docking microfluidic cell migration assay (D -Chip) for testing neutrophil chemotaxis and the memory effect.
[So] Source:Integr Biol (Camb);9(4):303-312, 2017 Apr 18.
[Is] ISSN:1757-9708
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Chemotaxis is a classic mechanism for guiding cell migration and an important topic in both fundamental cell biology and health sciences. Neutrophils are a widely used model to study eukaryotic cell migration and neutrophil chemotaxis itself can lead to protective or harmful immune actions to the body. While much has been learnt from past research about how neutrophils effectively navigate through a chemoattractant gradient, many interesting questions remain unclear. For example, while it is tempting to model neutrophil chemotaxis using the well-established biased random walk theory, the experimental proof was challenged by the cell's highly persistent migrating nature. A special experimental design is required to test the key predictions from the random walk model. Another question that has interested the cell migration community for decades concerns the existence of chemotactic memory and its underlying mechanism. Although chemotactic memory has been suggested in various studies, a clear quantitative experimental demonstration will improve our understanding of the migratory memory effect. Motivated by these questions, we developed a microfluidic cell migration assay (so-called dual-docking chip or D -Chip) that can test both the biased random walk model and the memory effect for neutrophil chemotaxis on a single chip enabled by multi-region gradient generation and dual-region cell alignment. Our results provide experimental support for the biased random walk model and chemotactic memory for neutrophil chemotaxis. Quantitative data analyses provide new insights into neutrophil chemotaxis and memory by making connections to entropic disorder, cell morphology and oscillating migratory response.
[Mh] Termos MeSH primário: Ensaios de Migração Celular
Quimiotaxia
Neutrófilos/citologia
[Mh] Termos MeSH secundário: Movimento Celular
Fatores Quimiotáticos
Simulação por Computador
Seres Humanos
Sistema Imunitário
Memória Imunológica
Técnicas Analíticas Microfluídicas
Neutrófilos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chemotactic Factors)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.1039/c7ib00037e


  8 / 5553 MEDLINE  
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[PMID]:28355725
[Au] Autor:An N; Chen SL; Li X
[Ti] Título:[A case report of immunoglonulin λ light chain amyloidosis combined with leukocyte cell-derived chemotaxin-2 amyloidosis].
[So] Source:Zhonghua Nei Ke Za Zhi;56(4):298-300, 2017 Apr 01.
[Is] ISSN:0578-1426
[Cp] País de publicação:China
[La] Idioma:chi
[Mh] Termos MeSH primário: Amiloidose/diagnóstico
Amiloidose/imunologia
Cadeias Leves de Imunoglobulina/imunologia
Cadeias lambda de Imunoglobulina
Leucócitos
[Mh] Termos MeSH secundário: Fatores Quimiotáticos
Seres Humanos
Masculino
Meia-Idade
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chemotactic Factors); 0 (Immunoglobulin Light Chains); 0 (Immunoglobulin lambda-Chains)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170330
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.0578-1426.2017.04.012


  9 / 5553 MEDLINE  
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[PMID]:28257236
[Au] Autor:Shariff S; Shelfoon C; Holden NS; Traves SL; Wiehler S; Kooi C; Proud D; Leigh R
[Ad] Endereço:1 Department of Physiology and Pharmacology, Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.
[Ti] Título:Human Rhinovirus Infection of Epithelial Cells Modulates Airway Smooth Muscle Migration.
[So] Source:Am J Respir Cell Mol Biol;56(6):796-803, 2017 Jun.
[Is] ISSN:1535-4989
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Airway remodeling, a characteristic feature of asthma, begins in early life. Recurrent human rhinovirus (HRV) infections are a potential inciting stimulus for remodeling. One component of airway remodeling is an increase in airway smooth muscle cell (ASMC) mass with a greater proximity of the ASMCs to the airway epithelium. We asked whether human bronchial epithelial cells infected with HRV produced mediators that are chemotactic for ASMCs. ASMC migration was investigated using the modified Boyden Chamber and the xCELLigence Real-Time Cell Analyzer (ACEA Biosciences Inc., San Diego, CA). Multiplex bead analysis was used to measure HRV-induced epithelial chemokine release. The chemotactic effects of CCL5, CXCL8, and CXCL10 were also examined. Supernatants from HRV-infected epithelial cells caused ASMC chemotaxis. Pretreatment of ASMCs with pertussis toxin abrogated chemotaxis, as did treatment with formoterol, forskolin, or 8-bromo-cAMP. CCL5, CXCL8, and CXCL10 were the most up-regulated chemokines produced by HRV-infected airway epithelial cells. When recombinant CCL5, CXCL8, and CXCL10 were used at levels found in epithelial supernatants, they induced ASMC chemotaxis similar to that seen with epithelial cell supernatants. When examined individually, CCL5 was the most effective chemokine in causing ASMC migration, and treatment of supernatant from HRV-infected epithelial cells with anti-CCL5 antibodies significantly attenuated ASMC migration. These findings suggest that HRV-induced CCL5 can induce ASMC chemotaxis and thus may contribute to the pathogenesis of airway remodeling in patients with asthma.
[Mh] Termos MeSH primário: Movimento Celular
Células Epiteliais/patologia
Células Epiteliais/virologia
Pulmão/patologia
Miócitos de Músculo Liso/patologia
Infecções por Picornaviridae/virologia
Rhinovirus/fisiologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Brônquios/patologia
Movimento Celular/efeitos dos fármacos
Quimiocina CCL5/metabolismo
Fatores Quimiotáticos/farmacologia
Meios de Cultivo Condicionados/farmacologia
AMP Cíclico/metabolismo
Células Epiteliais/efeitos dos fármacos
Células Epiteliais/metabolismo
Feminino
Citometria de Fluxo
Seres Humanos
Espaço Intracelular/metabolismo
Masculino
Meia-Idade
Peso Molecular
Miócitos de Músculo Liso/efeitos dos fármacos
Miócitos de Músculo Liso/metabolismo
Toxina Pertussis/toxicidade
Infecções por Picornaviridae/patologia
Rhinovirus/efeitos dos fármacos
Replicação Viral/efeitos dos fármacos
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chemokine CCL5); 0 (Chemotactic Factors); 0 (Culture Media, Conditioned); E0399OZS9N (Cyclic AMP); EC 2.4.2.31 (Pertussis Toxin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170304
[St] Status:MEDLINE
[do] DOI:10.1165/rcmb.2016-0252OC


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[PMID]:28185642
[Au] Autor:Francis EA; Heinrich V
[Ad] Endereço:Department of Biomedical Engineering, University of California at Davis, Davis, California.
[Ti] Título:Quantifying the Sensitivity of Human Immune Cells to Chemoattractant.
[So] Source:Biophys J;112(5):834-837, 2017 Mar 14.
[Is] ISSN:1542-0086
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The efficient recruitment of immune cells is a vital cornerstone of our defense against infections and a key challenge of immunotherapeutic applications. It relies on the ability of chemotaxing cells to prioritize their responses to different stimuli. For example, immune cells are known to abandon gradients of host-cell-produced cytokines in favor of complement-derived anaphylatoxins, which then guide the cells toward nearby pathogen surfaces. The aptitude to triage stimuli depends on the cells' specific sensitivities to different chemoattractants. We here use human neutrophils as uniquely capable biodetectors to map out the anaphylatoxic cloud that surrounds microbes in the presence of host serum. We quantify the neutrophil sensitivity in terms of the ratio between the chemoattractant concentration c and the production rate j of the chemoattractant at the source surface. An integrative experimental/theoretical approach allows us to estimate the c/j -threshold at which human neutrophils first detect nearby ß-glucan surfaces as c/j ≈ 0.0044 s/µm.
[Mh] Termos MeSH primário: Fatores Quimiotáticos/farmacologia
Neutrófilos/citologia
Neutrófilos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Quimiotaxia/efeitos dos fármacos
Complemento C5a/metabolismo
Seres Humanos
Neutrófilos/metabolismo
beta-Glucanas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chemotactic Factors); 0 (beta-Glucans); 80295-54-1 (Complement C5a)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170719
[Lr] Data última revisão:
170719
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE



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