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[PMID]:29360856
[Au] Autor:Yang Y; Xu H; Lu Y; Wang C; Lu Z
[Ad] Endereço:State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
[Ti] Título:Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin.
[So] Source:PLoS One;13(1):e0191686, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cnaphalocrocis medinalis (Guenée) is one of the important insect pests in rice field. Bt agents were recommended in the C. medinalis control and Bt rice is bred as a tactic to control this insect. However, the tolerance or resistance of insect to Bt protein is a main threat to the application of Bt protein. In order to investigate the response of C. medinalis transcriptome in defending a Cry1C toxin, high-through RNA-sequencing was carried in the C. medinalis larvae treated with and without Cry1C toxin. A total of 35,586 high-quality unigenes was annotated in the transcriptome of C. medinalis midgut. The comparative analysis identified 6,966 differently expressed unigenes (DEGs) between the two treatments. GO analysis showed that these genes involved in proteolysis and extracellular region. Among these DEGs, carboxylesterase, glutathione S-transferase and P450 were differently expressed in the treated C. medinalis midgut. Furthermore, trypsin, chymotrypsin, and carboxypeptidase were identified in DEGs, and most of them up-regulated. In addition, thirteen ABC transporters were downregulated and three upregulated in Cry1C-treated C. medinalis midgut. Based on the pathway analysis, antigen processing and presentation pathway, and chronic myeloid leukemia pathway were significant in C. medinalis treated with Cry1C toxin. These results indicated that serine protease, detoxification enzymes and ABC transporter, antigen processing and presentation pathway, and chronic myeloid leukemia pathway may involved in the response of C. medinalis to Cry1C toxin. This study provides a transcriptomal foundation for the identification and functional characterization of genes involved in the toxicity of Bt Cry protein against C. medinalis, and provides potential clues to the studies on the tolerance or resistance of an agriculturally important insect pest C. medinalis to Cry1C toxin.
[Mh] Termos MeSH primário: Proteínas de Bactérias/toxicidade
Endotoxinas/toxicidade
Proteínas Hemolisinas/toxicidade
Mariposas/efeitos dos fármacos
Transcriptoma
[Mh] Termos MeSH secundário: Animais
Mariposas/genética
Análise de Sequência de RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Endotoxins); 0 (Hemolysin Proteins); 0 (insecticidal crystal protein, Bacillus Thuringiensis)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180124
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191686


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[PMID]:28926817
[Au] Autor:Chen Y; Yang Y; Zhu H; Romeis J; Li Y; Peng Y; Chen X
[Ad] Endereço:The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
[Ti] Título:Safety of Bacillus thuringiensis Cry1C protein for Daphnia magna based on different functional traits.
[So] Source:Ecotoxicol Environ Saf;147:631-636, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Cry1C is a Bacillus thuringiensis (Bt) insecticidal protein and it can be produced by transgenic rice lines developed in China. Cladocera species are common aquatic arthropods that may be exposed to insecticidal proteins produced in Bt-transgenic plants through ingestion of pollen or crop residues in water. As the cladoceran Daphnia magna plays an important role in the aquatic food chain, it is important to assess the possible effects of Bt crops to this species. To evaluate the safety of the Cry1C protein for D. magna, individuals were exposed to different concentrations of purified Cry1C protein in M4 medium for 21 days. Potassium dichromate (K Cr O ), a known toxicant to D. magna, was added to M4 medium as a positive control treatment, and pure M4 medium was used as a negative control. Our results show that developmental, reproductive, and biochemical parameters of D. magna were not significantly different between Cry1C and negative control treatments but were significantly inhibited by the positive control. We thus conclude that D. magna is insensitive to Cry1C.
[Mh] Termos MeSH primário: Proteínas de Bactérias/toxicidade
Daphnia/efeitos dos fármacos
Endotoxinas/toxicidade
Proteínas Hemolisinas/toxicidade
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Proteínas de Bactérias/genética
China
Relação Dose-Resposta a Droga
Endotoxinas/genética
Proteínas Hemolisinas/genética
Oryza/genética
Oryza/metabolismo
Plantas Geneticamente Modificadas/genética
Plantas Geneticamente Modificadas/metabolismo
Testes de Toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Endotoxins); 0 (Hemolysin Proteins); 0 (Water Pollutants, Chemical); 0 (insecticidal crystal protein, Bacillus Thuringiensis)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170920
[St] Status:MEDLINE


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[PMID]:28457962
[Au] Autor:Khara JS; Obuobi S; Wang Y; Hamilton MS; Robertson BD; Newton SM; Yang YY; Langford PR; Ee PLR
[Ad] Endereço:Department of Pharmacy, National University of Singapore, 18 Science Drive 4, Singapore 117543, Singapore; Section of Paediatrics, Department of Medicine, St Mary's Campus, Imperial College London, London W2 1PG, United Kingdom; MRC Centre for Molecular Bacteriology and Infection, Department of Medi
[Ti] Título:Disruption of drug-resistant biofilms using de novo designed short α-helical antimicrobial peptides with idealized facial amphiphilicity.
[So] Source:Acta Biomater;57:103-114, 2017 Jul 15.
[Is] ISSN:1878-7568
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The escalating threat of antimicrobial resistance has increased pressure to develop novel therapeutic strategies to tackle drug-resistant infections. Antimicrobial peptides have emerged as a promising class of therapeutics for various systemic and topical clinical applications. In this study, the de novo design of α-helical peptides with idealized facial amphiphilicities, based on an understanding of the pertinent features of protein secondary structures, is presented. Synthetic amphiphiles composed of the backbone sequence (X Y Y X ) , where X and X are hydrophobic residues (Leu or Ile or Trp), Y and Y are cationic residues (Lys), and n is the number repeat units (2 or 2.5 or 3), demonstrated potent broad-spectrum antimicrobial activities against clinical isolates of drug-susceptible and multi-drug resistant bacteria. Live-cell imaging revealed that the most selective peptide, (LKKL) , promoted rapid permeabilization of bacterial membranes. Importantly, (LKKL) not only suppressed biofilm growth, but effectively disrupted mature biofilms after only 2h of treatment. The peptides (LKKL) and (WKKW) suppressed the production of LPS-induced pro-inflammatory mediators to levels of unstimulated controls at low micromolar concentrations. Thus, the rational design strategies proposed herein can be implemented to develop potent, selective and multifunctional α-helical peptides to eradicate drug-resistant biofilm-associated infections. STATEMENT OF SIGNIFICANCE: Antimicrobial peptides (AMPs) are increasingly explored as therapeutics for drug-resistant and biofilm-related infections to help expand the size and quality of the current antibiotic pipeline in the face of mounting antimicrobial resistance. Here, synthetic peptides rationally designed based upon principles governing the folding of natural α-helical AMPs, comprising the backbone sequence (X Y Y X ) , and which assemble into α-helical structures with idealized facial amphiphilicity, is presented. These multifunctional peptide amphiphiles demonstrate high bacterial selectivity, promote the disruption of pre-formed drug-resistant biofilms, and effectively neutralize endotoxins at low micromolar concentrations. Overall, the design strategies presented here could provide a useful tool for developing therapeutic peptides with broad-ranging clinical applications from the treatment and prevention of drug-resistant biofilms to the neutralization of bacterial endotoxins.
[Mh] Termos MeSH primário: Peptídeos Catiônicos Antimicrobianos/química
Bactérias/crescimento & desenvolvimento
Biofilmes
Farmacorresistência Bacteriana
Endotoxinas/química
[Mh] Termos MeSH secundário: Fenômenos Fisiológicos Bacterianos
Estrutura Secundária de Proteína
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antimicrobial Cationic Peptides); 0 (Endotoxins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


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[PMID]:29254304
[Au] Autor:Li JK; Wang C; Gong HD; Li HZ
[Ad] Endereço:Department of Neurosurgery, Affiliated HongQi Hospital of Mu Dan Jiang Medical University, Mudanjiang City, China.
[Ti] Título:Coagulation in hindbrain membrane meningioma patients treated with different injections using acute hypervolemic hemodilution.
[So] Source:J Biol Regul Homeost Agents;31(4):991-996, 2017 Oct-Dec.
[Is] ISSN:0393-974X
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to analyze the changes in coagulation in meningioma patients treated with different injections using the method of acute hypervolemic hemodilution (AHH). One hundred fifty hindbrain membrane meningioma patients were randomly divided into 5 groups, 30 per group. The first group were injected 40ml/time with Danhong after anesthesia induction; the second group were injected with 40ml~60ml/time Kangai and combined with interventional chemotherapy and embolization procedure; the third group of AHH were injected with polygeline 15ml/kg; the fourth group were injected with hydroxyethyl starch (130/0.4) sodium chloride in doses of 15ml/kg; the control group underwent basic treatment for lowering blood pressure and lowering blood fat. The changes of coagulation index were recorded before and after surgery and before and after the injection of different medications. Compared to the control group, for the first group of AHH, after being treated for 10 days and 30 days, the concentrations of bone specific alkaline phosphatase (BALP), bone Gla protein (BGP) and pro-collagen carboxy-terminal propeptide (PICP) were higher than that of the control group, the levels of endotoxin (ET) and C-reactive protein (CRP) were decreased compared to the control group (p less than 0.05); for the second group of AHH, after being treated for 10 days, the index of prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), fibrinogen (Fg) were not significantly changed, but the related level of vascular endothelial growth factor (VEGF) significantly decreased (p less than 0.05). Comparing the coagulation function index after surgery in the third and fourth groups, there were no significant changes in mean arterial pressure (MAP) level, heart rate (HR) value presented a low decrease, central venous pressure (CVP) level increased and the level of interleukin IL-6 showed a steady state after increasing. Analyzing the levels of interleukin IL-8 and tumor necrosis factor-α (TNF-α) after surgery, it was seen that in the third group they increased and in the fourth group they decreased (p less than 0.05). Danhong injection improved the coagulation function and microcirculation of patients, Kangai injection and interventional chemotherapy and embolization restrained the appearance of tumor angiogenesis, AHH operation with polygeline injection and hydroxyethyl starch (130/0.4) sodium chloride kept blood flow in normal parameters.
[Mh] Termos MeSH primário: Coagulação Sanguínea/efeitos dos fármacos
Cardiotônicos/uso terapêutico
Medicamentos de Ervas Chinesas/uso terapêutico
Hemodiluição/métodos
Neoplasias Meníngeas/tratamento farmacológico
Meningioma/tratamento farmacológico
[Mh] Termos MeSH secundário: Adulto
Fosfatase Alcalina/genética
Fosfatase Alcalina/metabolismo
Pressão Arterial/efeitos dos fármacos
Pressão Arterial/fisiologia
Biomarcadores/metabolismo
Viscosidade Sanguínea/efeitos dos fármacos
Proteína C-Reativa/genética
Proteína C-Reativa/metabolismo
Embolização Terapêutica/métodos
Endotoxinas/metabolismo
Feminino
Fibrinogênio/genética
Fibrinogênio/metabolismo
Expressão Gênica
Frequência Cardíaca/efeitos dos fármacos
Frequência Cardíaca/fisiologia
Seres Humanos
Derivados de Hidroxietil Amido/administração & dosagem
Masculino
Neoplasias Meníngeas/sangue
Neoplasias Meníngeas/patologia
Neoplasias Meníngeas/cirurgia
Meningioma/sangue
Meningioma/patologia
Meningioma/cirurgia
Meia-Idade
Osteocalcina/genética
Osteocalcina/metabolismo
Fragmentos de Peptídeos/genética
Fragmentos de Peptídeos/metabolismo
Substitutos do Plasma/administração & dosagem
Poligelina/administração & dosagem
Pró-Colágeno/genética
Pró-Colágeno/metabolismo
Rombencéfalo/efeitos dos fármacos
Rombencéfalo/metabolismo
Rombencéfalo/patologia
Rombencéfalo/cirurgia
Fator A de Crescimento do Endotélio Vascular/genética
Fator A de Crescimento do Endotélio Vascular/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Cardiotonic Agents); 0 (Drugs, Chinese Herbal); 0 (Endotoxins); 0 (Hydroxyethyl Starch Derivatives); 0 (Peptide Fragments); 0 (Plasma Substitutes); 0 (Procollagen); 0 (VEGFA protein, human); 0 (Vascular Endothelial Growth Factor A); 0 (danhong); 0 (procollagen type I carboxy terminal peptide); 104982-03-8 (Osteocalcin); 9001-32-5 (Fibrinogen); 9007-41-4 (C-Reactive Protein); 9015-56-9 (Polygeline); EC 3.1.3.1 (Alkaline Phosphatase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE


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[PMID]:29293334
[Au] Autor:Qiu Y; Li P; Dong S; Zhang X; Yang Q; Wang Y; Ge J; Hammock BD; Zhang C; Liu X
[Ad] Endereço:Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology,Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality (Ministry of Agriculture), Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural
[Ti] Título:Phage-Mediated Competitive Chemiluminescent Immunoassay for Detecting Cry1Ab Toxin by Using an Anti-Idiotypic Camel Nanobody.
[So] Source:J Agric Food Chem;66(4):950-956, 2018 Jan 31.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cry toxins have been widely used in genetically modified organisms for pest control, raising public concern regarding their effects on the natural environment and food safety. In this work, a phage-mediated competitive chemiluminescent immunoassay (c-CLIA) was developed for determination of Cry1Ab toxin using anti-idiotypic camel nanobodies. By extracting RNA from camels' peripheral blood lymphocytes, a naive phage-displayed nanobody library was established. Using anti-Cry1Ab toxin monoclonal antibodies (mAbs) against the library for anti-idiotypic antibody screening, four anti-idiotypic nanobodies were selected and confirmed to be specific for anti-Cry1Ab mAb binding. Thereafter, a c-CLIA was developed for detection of Cry1Ab toxin based on anti-idiotypic camel nanobodies and employed for sample testing. The results revealed a half-inhibition concentration of developed assay to be 42.68 ± 2.54 ng/mL, in the linear range of 10.49-307.1 ng/mL. The established method is highly specific for Cry1Ab recognition, with negligible cross-reactivity for other Cry toxins. For spiked cereal samples, the recoveries of Cry1Ab toxin ranged from 77.4% to 127%, with coefficient of variation of less than 9%. This study demonstrated that the competitive format based on phage-displayed anti-idiotypic nanobodies can provide an alternative strategy for Cry toxin detection.
[Mh] Termos MeSH primário: Anticorpos Anti-Idiotípicos
Proteínas de Bactérias/análise
Bacteriófagos
Camelus/imunologia
Endotoxinas/análise
Proteínas Hemolisinas/análise
Medições Luminescentes/métodos
Anticorpos de Domínio Único
[Mh] Termos MeSH secundário: Animais
Anticorpos Monoclonais
Proteínas de Bactérias/imunologia
Camelus/sangue
Grãos Comestíveis/química
Endotoxinas/imunologia
Contaminação de Alimentos/análise
Proteínas Hemolisinas/imunologia
Linfócitos/química
Biblioteca de Peptídeos
RNA/isolamento & purificação
Anticorpos de Domínio Único/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Anti-Idiotypic); 0 (Antibodies, Monoclonal); 0 (Bacterial Proteins); 0 (Endotoxins); 0 (Hemolysin Proteins); 0 (Peptide Library); 0 (Single-Domain Antibodies); 0 (insecticidal crystal protein, Bacillus Thuringiensis); 63231-63-0 (RNA)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04923


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[PMID]:29179781
[Au] Autor:Pei R; DiMarco DM; Putt KK; Martin DA; Gu Q; Chitchumroonchokchai C; White HM; Scarlett CO; Bruno RS; Bolling BW
[Ad] Endereço:1Department of Nutritional Sciences,University of Connecticut,3624 Horsebarn Road Extension,Unit 4017,Storrs,CT 06269,USA.
[Ti] Título:Low-fat yogurt consumption reduces biomarkers of chronic inflammation and inhibits markers of endotoxin exposure in healthy premenopausal women: a randomised controlled trial.
[So] Source:Br J Nutr;118(12):1043-1051, 2017 Dec.
[Is] ISSN:1475-2662
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The anti-inflammatory mechanisms of low-fat dairy product consumption are largely unknown. The objective of this study was to determine whether low-fat yogurt reduces biomarkers of chronic inflammation and endotoxin exposure in women. Premenopausal women (BMI 18·5-27 and 30-40 kg/m2) were randomised to consume 339 g of low-fat yogurt (yogurt non-obese (YN); yogurt obese (YO)) or 324 g of soya pudding (control non-obese; control obese (CO)) daily for 9 weeks (n 30/group). Fasting blood samples were analysed for IL-6, TNF-α/soluble TNF II (sTNF-RII), high-sensitivity C-reactive protein, 2-arachidonoyl glycerol, anandamide, monocyte gene expression, soluble CD14 (sCD14), lipopolysaccharide (LPS), LPS binding protein (LBP), IgM endotoxin-core antibody (IgM EndoCAb), and zonulin. BMI, waist circumference and blood pressure were also determined. After 9-week yogurt consumption, YO and YN had decreased TNF-α/sTNFR-RII. Yogurt consumption increased plasma IgM EndoCAb regardless of obesity status. sCD14 was not affected by diet, but LBP/sCD14 was lowered by yogurt consumption in both YN and YO. Yogurt intervention increased plasma 2-arachidonoylglycerol in YO but not YN. YO peripheral blood mononuclear cells expression of NF-κB inhibitor α and transforming growth factor ß1 increased relative to CO at 9 weeks. Other biomarkers were unchanged by diet. CO and YO gained approximately 0·9 kg in body weight. YO had 3·6 % lower diastolic blood pressure at week 3. Low-fat yogurt for 9 weeks reduced biomarkers of chronic inflammation and endotoxin exposure in premenopausal women compared with a non-dairy control food. This trial was registered as NCT01686204.
[Mh] Termos MeSH primário: Biomarcadores/sangue
Dieta
Endotoxinas/toxicidade
Inflamação/sangue
Inflamação/dietoterapia
Iogurte/análise
[Mh] Termos MeSH secundário: Proteínas da Fase Aguda
Adulto
Antropometria
Ácidos Araquidônicos/sangue
Proteína C-Reativa/metabolismo
Proteínas de Transporte/sangue
Doença Crônica
Citocinas/sangue
Gorduras na Dieta/administração & dosagem
Gorduras na Dieta/análise
Endocanabinoides/sangue
Endotoxemia/sangue
Endotoxemia/dietoterapia
Feminino
Glicerídeos/sangue
Seres Humanos
Imunoglobulina M/sangue
Leucócitos Mononucleares/metabolismo
Glicoproteínas de Membrana/sangue
Meia-Idade
NF-kappa B/metabolismo
Obesidade/metabolismo
Alcamidas Poli-Insaturadas/sangue
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Acute-Phase Proteins); 0 (Arachidonic Acids); 0 (Biomarkers); 0 (Carrier Proteins); 0 (Cytokines); 0 (Dietary Fats); 0 (Endocannabinoids); 0 (Endotoxins); 0 (Glycerides); 0 (Immunoglobulin M); 0 (Membrane Glycoproteins); 0 (NF-kappa B); 0 (Polyunsaturated Alkamides); 0 (lipopolysaccharide-binding protein); 8D239QDW64 (glyceryl 2-arachidonate); 9007-41-4 (C-Reactive Protein); UR5G69TJKH (anandamide)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[Cl] Clinical Trial:ClinicalTrial
[St] Status:MEDLINE
[do] DOI:10.1017/S0007114517003038


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[PMID]:29244909
[Au] Autor:Gordienko AI; Beloglazov VA; Kubyshkin AV
[Ti] Título:Changes of humoral anti-endotoxin immunity and low-intensity inflammation in diabetes mellitus type 1 and 2.
[So] Source:Patol Fiziol Eksp Ter;60(3):61-7, 2016 Jul-Sep.
[Is] ISSN:0031-2991
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:The purpose: The purpose. Investigate the levels of different classes serum anti-endotoxin antibodies in patients with diabetes mellitus type 1 and 2 and to hold the cluster analysis of the relationship between the individual levels of such antibodies and the concentration of C-reactive protein in the blood. Methods: We examined 51 patients with diabetes mellitus type 1 and 60 patients with diabetes mellitus type 2. The diagnosis of diabetes mellitus type 1 or type 2 has been delivered in accordance with the criteria of the World Health Organization. The control group included 49 healthy people who have not a history of any chronic disease, and the clinical manifestations of acute diseases were absent at the time of the survey. By sex and age, the control group of healthy people matched to a group of patients with diabetes type 1 and type 2. The concentration of C-reactive protein in the blood and the levels of serum anti-endotoxin antibodies of different classes (A, M and G) was determined by ELISA. Results: Using cluster analysis revealed that 40.8% of patients with type 1 diabetes increased concentration of C-reactive protein in the blood is associated with a significant reduction of levels of serum anti-endotoxin antibodies classes A, M and G. In 56.7% of patients with type 2 diabetes the high concentration of C-reactive protein in the blood levels of serum anti-endotoxin antibody classes A and M were not significantly different from the normal values, but the levels of serum anti-endotoxin antibodies of class G were significantly increased. The activation of inflammation with a further increase of C-reactive protein in the blood of patients with type 2 diabetes mellitus accompanied by a significant increase in levels of serum anti-endotoxin antibodies classes A and G, and also a tendency to reduce of levels anti-endotoxin antibodies class M. Conclusion: The results suggest about the relationship between low-intensity inflammation and immune response to enterobacterial endotoxins in patients with diabetes mellitus type 1 and 2.
[Mh] Termos MeSH primário: Anticorpos/imunologia
Diabetes Mellitus Tipo 1/imunologia
Diabetes Mellitus Tipo 2/imunologia
Endotoxinas/imunologia
Imunidade Humoral
[Mh] Termos MeSH secundário: Adulto
Anticorpos/sangue
Proteína C-Reativa/imunologia
Proteína C-Reativa/metabolismo
Diabetes Mellitus Tipo 1/sangue
Diabetes Mellitus Tipo 2/sangue
Feminino
Seres Humanos
Inflamação/sangue
Inflamação/imunologia
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies); 0 (Endotoxins); 9007-41-4 (C-Reactive Protein)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180118
[Lr] Data última revisão:
180118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE


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[PMID]:29215274
[Au] Autor:Zhang L; Zhao G; Hu X; Liu J; Li M; Batool K; Chen M; Wang J; Xu J; Huang T; Pan X; Xu L; Yu XQ; Guan X
[Ad] Endereço:Division of Cell Biology and Biophysics, University of Missouri-Kansas City , Kansas City, Missouri 64110, United States.
[Ti] Título:Cry11Aa Interacts with the ATP-Binding Protein from Culex quinquefasciatus To Improve the Toxicity.
[So] Source:J Agric Food Chem;65(50):10884-10890, 2017 Dec 20.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cry11Aa displays high toxicity to the larvae of several mosquito species, including Aedes, Culex, and Anopheles. To study its binding characterization against Culex quinquefasciatus, Cry11Aa was purified and western blot results showed that Cry11Aa could bind successfully to the brush border membrane vesicles. To identify Cry11Aa-binding proteins in C. quinquefasciatus, a biotin-based protein pull-down experiment was performed and seven Cry11Aa-binding proteins were isolated from the midgut of C. quinquefasciatus larvae. Analysis of liquid chromatography-tandem mass spectrometry showed that one of the Cry11Aa-binding proteins is the ATP-binding domain 1 family member B. To investigate its binding property and effect on the toxicity of Cry11Aa, western blot, far-western blot, enzyme-linked immunosorbent assay, and bioassays of Cry11Aa in the presence and absence of the recombinant ATP-binding protein were performed. Our results showed that the ATP-binding protein interacted with Cry11Aa and increased the toxicity of Cry11Aa against C. quinquefasciatus. Our study suggests that midgut proteins other than the toxin receptors may modulate the toxicity of Cry toxins against mosquitoes.
[Mh] Termos MeSH primário: Trifosfato de Adenosina/metabolismo
Proteínas de Bactérias/metabolismo
Culex/metabolismo
Endotoxinas/metabolismo
Proteínas Hemolisinas/metabolismo
Proteínas de Insetos/metabolismo
[Mh] Termos MeSH secundário: Animais
Proteínas de Bactérias/toxicidade
Culex/química
Culex/efeitos dos fármacos
Culex/genética
Endotoxinas/toxicidade
Proteínas Hemolisinas/toxicidade
Proteínas de Insetos/química
Proteínas de Insetos/genética
Larva/efeitos dos fármacos
Larva/genética
Larva/crescimento & desenvolvimento
Larva/metabolismo
Ligação Proteica
Domínios Proteicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Endotoxins); 0 (Hemolysin Proteins); 0 (Insect Proteins); 0 (insecticidal crystal protein, Bacillus Thuringiensis); 8L70Q75FXE (Adenosine Triphosphate)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180110
[Lr] Data última revisão:
180110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04427


  9 / 23095 MEDLINE  
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[PMID]:29211815
[Au] Autor:Berger AK; Yi H; Kearns DB; Mainou BA
[Ad] Endereço:Department of Pediatrics, Emory University School of Medicine, Atlanta, Georgia, United States of America.
[Ti] Título:Bacteria and bacterial envelope components enhance mammalian reovirus thermostability.
[So] Source:PLoS Pathog;13(12):e1006768, 2017 Dec.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Enteric viruses encounter diverse environments as they migrate through the gastrointestinal tract to infect their hosts. The interaction of eukaryotic viruses with members of the host microbiota can greatly impact various aspects of virus biology, including the efficiency with which viruses can infect their hosts. Mammalian orthoreovirus, a human enteric virus that infects most humans during childhood, is negatively affected by antibiotic treatment prior to infection. However, it is not known how components of the host microbiota affect reovirus infectivity. In this study, we show that reovirus virions directly interact with Gram positive and Gram negative bacteria. Reovirus interaction with bacterial cells conveys enhanced virion thermostability that translates into enhanced attachment and infection of cells following an environmental insult. Enhanced virion thermostability was also conveyed by bacterial envelope components lipopolysaccharide (LPS) and peptidoglycan (PG). Lipoteichoic acid and N-acetylglucosamine-containing polysaccharides enhanced virion stability in a serotype-dependent manner. LPS and PG also enhanced the thermostability of an intermediate reovirus particle (ISVP) that is associated with primary infection in the gut. Although LPS and PG alter reovirus thermostability, these bacterial envelope components did not affect reovirus utilization of its proteinaceous cellular receptor junctional adhesion molecule-A or cell entry kinetics. LPS and PG also did not affect the overall number of reovirus capsid proteins σ1 and σ3, suggesting their effect on virion thermostability is not mediated through altering the overall number of major capsid proteins on the virus. Incubation of reovirus with LPS and PG did not significantly affect the neutralizing efficiency of reovirus-specific antibodies. These data suggest that bacteria enhance reovirus infection of the intestinal tract by enhancing the thermal stability of the reovirus particle at a variety of temperatures through interactions between the viral particle and bacterial envelope components.
[Mh] Termos MeSH primário: Bacillus subtilis/fisiologia
Enterócitos/virologia
Escherichia coli K12/fisiologia
Infecções por Reoviridae/virologia
Reoviridae/fisiologia
[Mh] Termos MeSH secundário: Acetilglucosamina/análogos & derivados
Acetilglucosamina/metabolismo
Acetilglucosamina/toxicidade
Bacillus subtilis/metabolismo
Bacillus subtilis/ultraestrutura
Bacillus subtilis/virologia
Células CACO-2
Endotoxinas/metabolismo
Endotoxinas/toxicidade
Enterócitos/efeitos dos fármacos
Enterócitos/microbiologia
Enterócitos/patologia
Escherichia coli K12/metabolismo
Escherichia coli K12/ultraestrutura
Escherichia coli K12/virologia
Microbioma Gastrointestinal
Células HeLa
Temperatura Alta
Seres Humanos
Lipopolissacarídeos/metabolismo
Lipopolissacarídeos/toxicidade
Proteínas Luminescentes/genética
Proteínas Luminescentes/metabolismo
Microscopia Eletrônica de Transmissão
Peptidoglicano/metabolismo
Peptidoglicano/toxicidade
RNA/metabolismo
Estabilidade de RNA/efeitos dos fármacos
Proteínas Recombinantes/metabolismo
Reoviridae/química
Reoviridae/efeitos dos fármacos
Reoviridae/patogenicidade
Infecções por Reoviridae/metabolismo
Infecções por Reoviridae/microbiologia
Infecções por Reoviridae/patologia
Ácidos Teicoicos/metabolismo
Ácidos Teicoicos/toxicidade
Vírion/química
Vírion/patogenicidade
Vírion/fisiologia
Ligação Viral/efeitos dos fármacos
Internalização do Vírus/efeitos dos fármacos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Endotoxins); 0 (Lipopolysaccharides); 0 (Luminescent Proteins); 0 (Peptidoglycan); 0 (RNA, recombinant); 0 (Recombinant Proteins); 0 (Teichoic Acids); 0 (red fluorescent protein); 56411-57-5 (lipoteichoic acid); 63231-63-0 (RNA); 67924-63-4 (endotoxin, Escherichia coli); V956696549 (Acetylglucosamine)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180109
[Lr] Data última revisão:
180109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006768


  10 / 23095 MEDLINE  
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[PMID]:29211772
[Au] Autor:Spierenburg EAJ; Smit LAM; Krop EJM; Heederik D; Hylkema MN; Wouters IM
[Ad] Endereço:Institute for Risk Assessment Sciences, Division of Environmental Epidemiology, Utrecht University, Utrecht, the Netherlands.
[Ti] Título:Occupational endotoxin exposure in association with atopic sensitization and respiratory health in adults: Results of a 5-year follow-up.
[So] Source:PLoS One;12(12):e0189097, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The objective of the present longitudinal study was to investigate the effects of occupational endotoxin exposure on respiratory health and atopic sensitization in adults. Health outcomes and personal endotoxin exposure estimates were determined for 234 farmers and agricultural workers both at baseline and 5 years later. A questionnaire was used to assess respiratory symptoms, spirometry tests were performed and total and specific IgE levels were measured in serum. A twofold increase in personal endotoxin exposure was associated with less hay fever (OR 0.68, 95%CI 0.54-0.87) and grass IgE positivity (OR 0.81, 95%CI 0.68-0.97) at both time points ("persistent" versus "never"). Although not statistically significant, a consistent protective pattern was observed for an increased loss of hay fever symptoms (OR 2.19, 95%CI 0.96-4.99) and grass IgE positivity (OR 1.24, 95%CI 0.76-2.02), and for less new-onset of hay fever (OR 0.87, 95%CI 0.65-1.17), grass IgE positivity (OR 0.83, 95%CI 0.61-1.12) and atopic sensitization (OR 0.75, 95%CI 0.55-1.02). Endotoxin exposure was not associated with changes in lung function. We showed that occupational endotoxin exposure is associated with a long-term protective effect on hay fever and grass IgE positivity. Results on longitudinal changes in hay fever, atopy and grass IgE positivity in adulthood were consistent with a protective effect of endotoxin exposure, but results need to be confirmed in larger cohorts. An effect of endotoxin exposure on lung function decline was not found.
[Mh] Termos MeSH primário: Endotoxinas/administração & dosagem
Hipersensibilidade Imediata
Exposição Ocupacional
Sistema Respiratório/efeitos dos fármacos
[Mh] Termos MeSH secundário: Adulto
Estudos de Coortes
Feminino
Seguimentos
Seres Humanos
Imunoglobulina E/sangue
Masculino
Meia-Idade
Testes de Função Respiratória
Sistema Respiratório/fisiopatologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Endotoxins); 37341-29-0 (Immunoglobulin E)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189097



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