Base de dados : MEDLINE
Pesquisa : D26.776 [Categoria DeCS]
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  1 / 51253 MEDLINE  
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[PMID]:29377914
[Au] Autor:Grasso R; Musumeci F; Gulino M; Scordino A
[Ad] Endereço:Department of Physics and Astronomy, Catania University, Catania, Italy.
[Ti] Título:Exploring the behaviour of water in glycerol solutions by using delayed luminescence.
[So] Source:PLoS One;13(1):e0191861, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The crucial role of water in the engine of life have encouraged many researchers in studying, both theoretically and experimentally, the possible "structure" of water. Many properties of water have been related to the interplay between two distinct and interconverting structural species, namely the low-density water (LDW) and the high-density water (HDW). Supported by the results obtained with other aqueous solutions, this paper deals with the possibility of using the ultra-weak delayed luminescence (DL) to investigate water structuring in a mixture with glycerol, characterized only by hydrogen bonds between the various molecules. Spectral and temporal characteristics of DL decays give information on the two components of the mixture, by evidencing the contribution of water at glycerol concentrations close to the values used in cryopreservation. DL results have shown a correlation with LDW clusters size as determined by other researchers on the basis of neutron diffraction experiments and computational modelling, as reported in Literature.
[Mh] Termos MeSH primário: Glicerol/química
Água/química
[Mh] Termos MeSH secundário: Luminescência
Soluções
Análise Espectral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Solutions); 059QF0KO0R (Water); PDC6A3C0OX (Glycerol)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180130
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191861


  2 / 51253 MEDLINE  
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[PMID]:29367490
[Au] Autor:Asakawa T; Takano Y; Ohta A; Asakawa H
[Ad] Endereço:School of Chemistry, College of Science and Engineering, Kanazawa University.
[Ti] Título:Aggregation and pH Responsive Behavior of Thioester Surfactants and Formation of Disulfide Linkages in Aqueous Solutions.
[So] Source:J Oleo Sci;67(2):199-206, 2018 Feb 01.
[Is] ISSN:1347-3352
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:pH responsive surfactants, [C H N(CH ) (CH ) SCOCH ]Br (C nSAc, n = 4, 11, 12), were prepared, and their properties in aqueous solution were examined. The critical micelle concentration (cmc) and critical vesicle concentration (cvc) were determined based on changes in conductivity, as well as by fluorescence measurements, and light scattering methods. A significant increase in the light scattering intensities of the C nSAc (n=11, 12) systems suggested that the growth of aggregates was accompanied by considerable counterion binding with increasing surfactant concentration. The diameter of C 11SAc, recorded by the dynamic light scattering measurements, was about 9.6 ±1.0 nm, which was slightly smaller than that for didodecyldimethylammonium bromide (DDAB) vesicles. The thioester group was easily hydrolyzed upon the addition of NaOH, while it was hardly hydrolyzed with the addition of HCl. The time course of alkaline hydrolysis was examined by the conductivity measurements and high-performance liquid chromatography analysis. [C H N(CH ) (CH ) SS(CH ) N(CH ) C H ]2Br (2C 11SS) was generated in the C 11SAc alkaline solution because of air oxidation. The C 11SAc alkaline solution gradually became an opaque blue color with increasing light scattering at 346 nm, indicating the remarkable growth of vesicles. The chemical structure of 2C 11SS was consistent with that of a disulfide linked double tailed surfactant, similar to DDAB. The disulfide linkage between the double tailed surfactants will contribute to the stabilization and growth of vesicles.
[Mh] Termos MeSH primário: Ésteres/química
Tensoativos/química
Água/química
[Mh] Termos MeSH secundário: Dissulfetos/química
Difusão Dinâmica da Luz
Concentração de Íons de Hidrogênio
Hidrólise
Micelas
Oxirredução
Compostos de Amônio Quaternário/química
Soluções
Tensão Superficial
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Disulfides); 0 (Esters); 0 (Micelles); 0 (Quaternary Ammonium Compounds); 0 (Solutions); 0 (Surface-Active Agents); 059QF0KO0R (Water); 13146-86-6 (didodecyldimethylammonium)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE
[do] DOI:10.5650/jos.ess17192


  3 / 51253 MEDLINE  
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[PMID]:29175399
[Au] Autor:Stubbings WA; Harrad S
[Ad] Endereço:School of Geography, Earth, & Environmental Sciences, University of Birmingham, Birmingham, B15 2TT, UK. Electronic address: william.a.stubbings@gmail.com.
[Ti] Título:Leaching of TCIPP from furniture foam is rapid and substantial.
[So] Source:Chemosphere;193:720-725, 2018 Feb.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A series of laboratory experiments were conducted, in which waste furniture polyurethane foam samples containing tris (1-chloro-2-propyl) phosphate (TCIPP) were contacted with a range of leaching fluids, formulated to simulate the composition of landfill leachate. Leaching was examined under a number of different scenarios, such as: dissolved humic matter concentration, pH, and temperature, as well as the effect of agitation, and waste:leaching fluid contact duration. In addition to single batch (no replenishment of leaching fluid), serial batch (draining of leachate and replenishment with fresh leaching fluid at various time intervals) experiments were conducted. Leaching of TCIPP from PUF appears to be a first order process. Concentrations of TCIPP in leachate generated by the experiments in this study ranged from 13 mg L to 130 mg L . In serial batch leaching experiments, >95% of TCIPP was depleted from PUF after 168 h total contact with leaching fluid. Our experiments indicate leaching is potentially a very significant pathway of TCIPP emissions to the environment.
[Mh] Termos MeSH primário: Poluição Ambiental
Retardadores de Chama/toxicidade
Decoração de Interiores e Mobiliário
Organofosfatos/química
Poliuretanos/toxicidade
Poluentes Químicos da Água/química
[Mh] Termos MeSH secundário: Cinética
Solubilidade
Soluções/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Flame Retardants); 0 (Organophosphates); 0 (Polyurethanes); 0 (Solutions); 0 (Water Pollutants, Chemical); 9009-54-5 (polyurethane foam)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


  4 / 51253 MEDLINE  
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[PMID]:28938140
[Au] Autor:Yi X; He J; Guo Y; Han Z; Yang M; Jin J; Gu J; Ou M; Xu X
[Ad] Endereço:College of Chemistry, Fuzhou University, Fuzhou 350108, China. Electronic address: 927208329@qq.com.
[Ti] Título:Encapsulating Fe O into calcium alginate coated chitosan hydrochloride hydrogel beads for removal of Cu (II) and U (VI) from aqueous solutions.
[So] Source:Ecotoxicol Environ Saf;147:699-707, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The aim of this work was to study the removal of Cu (II) and U (VI) ions from aqueous solutions by encapsulating magnetic Fe O nanoparticles into calcium alginate coated chitosan hydrochloride (CCM) hydrogel beads. ATR-FTIR and XRD analysis data indicated that the CCM composites were successfully prepared. SEM images and EDX spectra showed that Cu and UO ions were adhered onto sorbents. Adsorption properties for removal of both copper and uranium ions under various experimental conditions were investigated. Kinetic data and sorption equilibrium isotherms were also conducted in batch process. The sorption kinetic analysis revealed that sorption of Cu (II) and U (VI) followed the pseudo-second-order model well and exhibited 3-stage intraparticle diffusion model during the whole sorption process. Equilibrium data were best described by Langmuir model, and the CCM composite hydrogel beads showed the estimated maximum adsorption capacity 143.276mg/g and 392.692mg/g for Cu (II) and U (VI), respectively. The CCM adsorbent exhibited excellent reusability for five cycles use without significant changes in the adsorption capacity and structural stability. The results demonstrated that CCM can be an effective and promising sorbent for Cu (II) and U (VI) ions in wastewater.
[Mh] Termos MeSH primário: Alginatos/química
Quitosana/química
Cobre/análise
Nanopartículas de Magnetita/química
Compostos de Urânio/análise
Poluentes Químicos da Água/análise
Purificação da Água/métodos
[Mh] Termos MeSH secundário: Adsorção
Ácido Glucurônico/química
Ácidos Hexurônicos/química
Hidrogéis/química
Concentração de Íons de Hidrogênio
Íons
Cinética
Modelos Teóricos
Soluções
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alginates); 0 (Hexuronic Acids); 0 (Hydrogels); 0 (Ions); 0 (Magnetite Nanoparticles); 0 (Solutions); 0 (Uranium Compounds); 0 (Water Pollutants, Chemical); 789U1901C5 (Copper); 8A5D83Q4RW (Glucuronic Acid); 8C3Z4148WZ (alginic acid); 9012-76-4 (Chitosan)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170923
[St] Status:MEDLINE


  5 / 51253 MEDLINE  
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[PMID]:28467316
[Au] Autor:Chen R; Cai X; Ma K; Zhou Y; Wang Y; Jiang T
[Ad] Endereço:The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, People's Republic of China.
[Ti] Título:The fabrication of double-layered chitosan/gelatin/genipin nanosphere coating for sequential and controlled release of therapeutic proteins.
[So] Source:Biofabrication;9(2):025028, 2017 Jun 01.
[Is] ISSN:1758-5090
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bone regeneration is a complicated process and includes a number of distinct and sequential stages of coordinated cellular actions under the regulation of multiple growth factors. Therefore, bone grafting materials in which growth factors can be incorporated and released in a programmed order in line with the bone tissue healing process may lead to desirable clinical outcomes. In the present study, a double-layered chitosan/gelatin/genipin (d-CSG/G) nanosphere coating is developed by using layer-by-layer electrophoretic deposition and genipin crosslinking. The surface morphology, physicochemical and mechanical properties of the coatings are explored. Cytochrome C is used as a therapeutic model protein and is successfully loaded on the inner and outer layers of the coating. The protein release can be controlled by the loading position, genipin concentration and thickness of the outer layer. Furthermore, the cell response to the coatings was evaluated. Real-time polymerase chain reactions, immunofluorescence staining and extracellular matrix mineralization assay confirmed that the functions of the loaded growth factor are fully preserved after fabrication. Overall, the d-CSG/G nanosphere coating could be a promising growth factor delivery system to promote bone tissue regeneration.
[Mh] Termos MeSH primário: Biomimética/métodos
Quitosana/química
Materiais Revestidos Biocompatíveis/química
Citocromos c/uso terapêutico
Gelatina/química
Iridoides/química
Nanosferas/química
[Mh] Termos MeSH secundário: Animais
Proteína Morfogenética Óssea 2/química
Calcificação Fisiológica
Bovinos
Reagentes para Ligações Cruzadas/química
Preparações de Ação Retardada
Matriz Extracelular/metabolismo
Imunofluorescência
Células Mesenquimais Estromais/citologia
Nanosferas/ultraestrutura
Osteocalcina/metabolismo
Ratos
Reação em Cadeia da Polimerase em Tempo Real
Proteínas Recombinantes/química
Soluções
Espectroscopia de Infravermelho com Transformada de Fourier
Propriedades de Superfície
Fator de Crescimento Transformador beta/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bone Morphogenetic Protein 2); 0 (Coated Materials, Biocompatible); 0 (Cross-Linking Reagents); 0 (Delayed-Action Preparations); 0 (Iridoids); 0 (Recombinant Proteins); 0 (Solutions); 0 (Transforming Growth Factor beta); 0 (recombinant human bone morphogenetic protein-2); 104982-03-8 (Osteocalcin); 9000-70-8 (Gelatin); 9007-43-6 (Cytochromes c); 9012-76-4 (Chitosan); A3V2NE52YG (genipin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1088/1758-5090/aa70c3


  6 / 51253 MEDLINE  
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[PMID]:28455252
[Au] Autor:Kwak HW; Shin M; Lee JY; Yun H; Song DW; Yang Y; Shin BS; Park YH; Lee KH
[Ad] Endereço:Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
[Ti] Título:Fabrication of an ultrafine fish gelatin nanofibrous web from an aqueous solution by electrospinning.
[So] Source:Int J Biol Macromol;102:1092-1103, 2017 Sep.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Electrospinning of aqueous gelatin solution obtained from bovine or porcine sources has been difficult to achieve without additional facilities, such as a temperature control oven or heating cover. Gelatin from cold-water fish has low contents of proline (Pro) and hydroxyproline (Hyp) compared with mammalian-derived gelatin. For this reason, the fish-derived gelatin maintains a sol state without showing gelation behavior at room temperature. In the present study, we prepared an ultrafine fish gelatin nanofibrous web by electrospinning from aqueous solutions without any additive polymers or temperature control facilities. The concentration and viscosity of fish gelatin are the most important factor in determining the electrospinnability and fiber diameter. Electrospinning of aqueous fish gelatin has the highest nanofiber productivity compared to other organic solvent systems. Using glutaraldehyde vapor (GTA), the water stability was improved and substantial enhancement was achieved in the mechanical properties. Finally, the cytotoxicity of a fish gelatin nanofibrous scaffold was evaluated based on a cell proliferation study by culturing human dermal fibroblasts (HDFs) compared with a fish gelatin film and nanofibrous mat from mammalian gelatin. The result shows better initial cell attachment and proliferation compared with the fish gelatin film and no significant difference compared with mammalian-derived gelatin nanofibrous mat. We expect that electrospinning of aqueous fish gelatin could be an effective alternative mammalian gelatin source.
[Mh] Termos MeSH primário: Eletricidade
Peixes
Gelatina/química
Nanofibras/química
Nanotecnologia
Água/química
[Mh] Termos MeSH secundário: Animais
Materiais Biocompatíveis/química
Materiais Biocompatíveis/farmacologia
Bovinos
Adesão Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Fibroblastos/citologia
Fibroblastos/efeitos dos fármacos
Gelatina/farmacologia
Glutaral/química
Seres Humanos
Hidrólise
Reologia
Soluções
Viscosidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Solutions); 059QF0KO0R (Water); 9000-70-8 (Gelatin); T3C89M417N (Glutaral)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


  7 / 51253 MEDLINE  
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[PMID]:28968940
[Au] Autor:Li Z; Wang P; Menzies NW; Kopittke PM
[Ad] Endereço:The University of Queensland, School of Agriculture and Food Sciences, St. Lucia, Queensland, 4072, Australia.
[Ti] Título:Defining appropriate methods for studying toxicities of trace metals in nutrient solutions.
[So] Source:Ecotoxicol Environ Saf;147:872-880, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The use of inappropriate experimental conditions for examining trace metal phytotoxicity results in data of questionable value. The present study aimed to identify suitable parameters for study of phytotoxic metals in nutrient solutions. First, the literature was reviewed to determine the concentration of six metals (Cd, Cu, Hg, Ni, Pb, and Zn) from solution of contaminated soils. Next, the effects of pH, P, Cl, NO , and four Fe-chelators were investigated by using thermodynamic modelling and by examining changes in root elongation rate of soybean (Glycine max cv. Bunya). The literature review identified that the solution concentrations of metals in soils were low, ranging from (µM) 0.069-11Cd, 0.19-15.8 Cu, 0.000027-0.000079 Hg, 1.0-8.7 Ni, 0.004-0.55 Pb, and 0.4-36.3 Zn. For studies in nutrient solution, pH should generally be low given its effects on solubility and speciation, as should the P concentration due to the formation of insoluble phosphate salts. The concentrations of Cl, NO , and various chelators also influence metal toxicity through alteration of metal speciation. The nutrient solutions used to study metal toxicity should consider environmentally-relevant conditions especially for metal concentrations, with concentrations of other components added at levels that do not substantially alter metal toxicity.
[Mh] Termos MeSH primário: Monitoramento Ambiental/métodos
Hidroponia
Metais Pesados/toxicidade
Feijão de Soja/efeitos dos fármacos
Oligoelementos/toxicidade
[Mh] Termos MeSH secundário: Metais Pesados/análise
Modelos Teóricos
Raízes de Plantas/efeitos dos fármacos
Raízes de Plantas/crescimento & desenvolvimento
Solubilidade
Soluções
Feijão de Soja/crescimento & desenvolvimento
Termodinâmica
Oligoelementos/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Metals, Heavy); 0 (Solutions); 0 (Trace Elements)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171004
[St] Status:MEDLINE


  8 / 51253 MEDLINE  
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[PMID]:29441996
[Au] Autor:Ayoub BM; Abdel-Aziz O
[Ti] Título:A guide for using experimental design in chromatographic method development: applied to the analysis of selected anti-diabetic pharmaceutical combinations.
[So] Source:Pharmazie;71(12):683-690, 2016 Dec 01.
[Is] ISSN:0031-7144
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A guide experimental design in chromatographic method development was described and applied successfully to the analysis of different recently approved anti-diabetic pharmaceutical combinations. Enhancement of UHPLC analysis of alogliptin benzoate either with pioglitazone hydrochloride or with metformin hydrochloride was achieved. The optimal chromatographic conditions were not attained by trial and error that requires a large number of experiments. Alternatively, a computer program was used as a systematic optimization strategy for the design of the experiment which accurately predicts the combined effect of different factors simultaneously. Resolution between peaks was studied by the proposed fractional factorial design approach performed by the Minitab® Program using screening and optimization steps. Application of the central composite design was implemented. A Pareto chart was used to exclude the insignificant variables. Linearity ranges were found to be 0.5-40 µg ml-1, 1-20 µg ml-1 and 1-32 µg ml-1 for alogliptin benzoate, pioglitazone hydrochloride and metformin hydrochloride, respectively. The proposed method is applicable for the analysis of six pharmaceutical dosage forms namely, Nesina®, Actos®, Glucophage®, Oseni®, Kazano® and Actoplus MET® tablets.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Hipoglicemiantes/análise
[Mh] Termos MeSH secundário: Formas de Dosagem
Combinação de Medicamentos
Desenho de Equipamento
Metformina/análise
Piperidinas/análise
Padrões de Referência
Reprodutibilidade dos Testes
Soluções
Comprimidos/análise
Tiazolidinedionas/análise
Uracila/análogos & derivados
Uracila/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dosage Forms); 0 (Drug Combinations); 0 (Hypoglycemic Agents); 0 (Piperidines); 0 (Solutions); 0 (Tablets); 0 (Thiazolidinediones); 56HH86ZVCT (Uracil); 9100L32L2N (Metformin); JHC049LO86 (alogliptin); X4OV71U42S (pioglitazone)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180215
[St] Status:MEDLINE
[do] DOI:10.1691/ph.2016.6095


  9 / 51253 MEDLINE  
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[PMID]:28471110
[Au] Autor:Luo Y; Li WL; Huang WH; Liu XH; Song YG; Qu HB
[Ad] Endereço:Pharmaceutical Informatics Institute, Zhejiang University, Hangzhou 310058, China.
[Ti] Título:Rapid quantification of multi-components in alcohol precipitation liquid of Codonopsis Radix using near infrared spectroscopy (NIRS).
[So] Source:J Zhejiang Univ Sci B;18(5):383-392, 2017 May.
[Is] ISSN:1862-1783
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:A near infrared spectroscopy (NIRS) approach was established for quality control of the alcohol precipitation liquid in the manufacture of Codonopsis Radix. By applying NIRS with multivariate analysis, it was possible to build variation into the calibration sample set, and the Plackett-Burman design, Box-Behnken design, and a concentrating-diluting method were used to obtain the sample set covered with sufficient fluctuation of process parameters and extended concentration information. NIR data were calibrated to predict the four quality indicators using partial least squares regression (PLSR). In the four calibration models, the root mean squares errors of prediction (RMSEPs) were 1.22 µg/ml, 10.5 µg/ml, 1.43 µg/ml, and 0.433% for lobetyolin, total flavonoids, pigments, and total solid contents, respectively. The results indicated that multi-components quantification of the alcohol precipitation liquid of Codonopsis Radix could be achieved with an NIRS-based method, which offers a useful tool for real-time release testing (RTRT) of intermediates in the manufacture of Codonopsis Radix.
[Mh] Termos MeSH primário: Codonopsis/química
Medicamentos de Ervas Chinesas/química
Etanol/química
Precipitação Fracionada/métodos
Modelos Estatísticos
Extratos Vegetais/química
Espectroscopia de Luz Próxima ao Infravermelho/métodos
[Mh] Termos MeSH secundário: Misturas Complexas/análise
Misturas Complexas/química
Simulação por Computador
Medicamentos de Ervas Chinesas/análise
Modelos Químicos
Extratos Vegetais/análise
Soluções/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Complex Mixtures); 0 (Drugs, Chinese Herbal); 0 (Plant Extracts); 0 (Solutions); 3K9958V90M (Ethanol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1631/jzus.B1600141


  10 / 51253 MEDLINE  
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[PMID]:28467708
[Au] Autor:Watanabe H; Ono M; Ariyoshi T; Katayanagi R; Saji H
[Ad] Endereço:Department of Patho-Functional Bioanalysis, Graduate School of Pharmaceutical Sciences, Kyoto University , 46-29 Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan.
[Ti] Título:Novel Benzothiazole Derivatives as Fluorescent Probes for Detection of ß-Amyloid and α-Synuclein Aggregates.
[So] Source:ACS Chem Neurosci;8(8):1656-1662, 2017 08 16.
[Is] ISSN:1948-7193
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Deposits of ß-amyloid (Aß) and α-synuclein (α-syn) are the hallmark of Alzheimer's disease (AD) and Parkinson's disease (PD), respectively. The detection of these protein aggregates with fluorescent probes is particularly of interest for preclinical studies using fluorescence microscopy on human brain tissue. In this study, we newly designed and synthesized three push-pull benzothiazole (PP-BTA) derivatives as fluorescent probes for detection of Aß and α-syn aggregates. Fluorescence intensity of all PP-BTA derivatives significantly increased upon binding to Aß(1-42) and α-syn aggregates in solution. In in vitro saturation binding assays, PP-BTA derivatives demonstrated affinity for both Aß(1-42) (K = 40-148 nM) and α-syn (K = 48-353 nM) aggregates. In particular, PP-BTA-4 clearly stained senile plaques composed of Aß aggregates in the AD brain section. Moreover, it also labeled Lewy bodies composed of α-syn aggregates in the PD brain section. These results suggest that PP-BTA-4 may serve as a promising fluorescent probe for the detection of Aß and α-syn aggregates.
[Mh] Termos MeSH primário: Peptídeos beta-Amiloides/metabolismo
Benzotiazóis
Encéfalo/metabolismo
Encéfalo/patologia
Corantes Fluorescentes
Fragmentos de Peptídeos/metabolismo
alfa-Sinucleína/metabolismo
[Mh] Termos MeSH secundário: Idoso
Doença de Alzheimer/metabolismo
Doença de Alzheimer/patologia
Benzotiazóis/síntese química
Benzotiazóis/química
Feminino
Corantes Fluorescentes/síntese química
Corantes Fluorescentes/química
Seres Humanos
Imuno-Histoquímica
Corpos de Lewy/metabolismo
Corpos de Lewy/patologia
Masculino
Estrutura Molecular
Doença de Parkinson/metabolismo
Doença de Parkinson/patologia
Placa Amiloide/metabolismo
Placa Amiloide/patologia
Ligação Proteica
Soluções
Espectrometria de Fluorescência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amyloid beta-Peptides); 0 (Benzothiazoles); 0 (Fluorescent Dyes); 0 (Peptide Fragments); 0 (SNCA protein, human); 0 (Solutions); 0 (alpha-Synuclein); 0 (amyloid beta-protein (1-42))
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1021/acschemneuro.6b00450



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