Base de dados : MEDLINE
Pesquisa : D26.776.399 [Categoria DeCS]
Referências encontradas : 2961 [refinar]
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[PMID]:28543431
[Au] Autor:Wang M; Chai N; Sha B; Guo M; Zhuang J; Xu F; Li F
[Ad] Endereço:The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, P. R. China.
[Ti] Título:The effect of substrate stiffness on cancer cell volume homeostasis.
[So] Source:J Cell Physiol;233(2):1414-1423, 2018 Feb.
[Is] ISSN:1097-4652
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Existing studies on the mechanism of cell volume regulation are mainly relevant to ion channels and osmosis in extracellular fluid. Recently, accumulating evidence has shown that cellular mechanical microenvironment also influences the cell volume. Herein, we investigated the regulation of substrate stiffness on the cell volume homeostasis of MCF-7 cells and their following migration behaviors. We found that cell volume increases with increasing substrate stiffness, which could be affected by blocking the cell membrane anion permeability and dopamine receptor. In addition, the cell migration is significantly inhibited by decreasing the cell volume using tamoxifen and such inhibition effect on migration is enhanced by increasing substrate stiffness. The cell membrane anion permeability might be the linker between cellular mechanical microenvironment and cellular volume homeostasis regulation. This work revealed the regulation of substrate stiffness on cell volume homeostasis for the first time, which would provide a new perspective into the understanding of cancer metastasis and a promising anti-cancer therapy through regulation of cell volume homeostasis.
[Mh] Termos MeSH primário: Resinas Acrílicas/química
Neoplasias da Mama/metabolismo
Membrana Celular/metabolismo
Tamanho Celular
Osmorregulação
[Mh] Termos MeSH secundário: Ácido 4,4'-Di-Isotiocianoestilbeno-2,2'-Dissulfônico/farmacologia
Proteínas de Transporte de Ânions/antagonistas & inibidores
Proteínas de Transporte de Ânions/metabolismo
Antineoplásicos Hormonais/farmacologia
Benzazepinas/farmacologia
Neoplasias da Mama/tratamento farmacológico
Neoplasias da Mama/patologia
Adesão Celular
Membrana Celular/efeitos dos fármacos
Membrana Celular/patologia
Permeabilidade da Membrana Celular
Movimento Celular
Tamanho Celular/efeitos dos fármacos
Colágeno/metabolismo
Feminino
Seres Humanos
Hidrogéis
Soluções Hipotônicas/farmacologia
Células MCF-7
Osmorregulação/efeitos dos fármacos
Porosidade
Receptores de Dopamina D1/antagonistas & inibidores
Receptores de Dopamina D1/metabolismo
Tamoxifeno/farmacologia
Microambiente Tumoral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acrylic Resins); 0 (Anion Transport Proteins); 0 (Antineoplastic Agents, Hormonal); 0 (Benzazepines); 0 (DRD1 protein, human); 0 (Hydrogels); 0 (Hypotonic Solutions); 0 (Receptors, Dopamine D1); 0 (SCH 23390); 094ZI81Y45 (Tamoxifen); 9003-05-8 (polyacrylamide); 9007-34-5 (Collagen); Q1O6DSW23R (4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1002/jcp.26026


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[PMID]:28499095
[Au] Autor:Kim JM; Choi S; Park K
[Ad] Endereço:1 Department of Physiology, College of Dentistry, Seoul National University, & Dental Research Institute, Seoul, Korea.
[Ti] Título:TRPM7 Is Involved in Volume Regulation in Salivary Glands.
[So] Source:J Dent Res;96(9):1044-1050, 2017 Aug.
[Is] ISSN:1544-0591
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Under hypotonic conditions, the regulatory volume decrease (RVD) is essential to maintain physiological homeostasis and functions in diverse biological systems. Intracellular Ca has been reported as an important mediator of this response, but the underlying Ca mechanism responsible for RVD is still controversial. Here we investigate the role of Ca in the RVD response using live-cell imaging, microspectrofluorimetry, and a patch-clamp technique. A typical RVD was observed in submandibular gland acinar cells after swelling in a hypotonic solution, whereas intracellular Ca chelation completely inhibited the RVD response. The incidence and magnitude of the Ca transient were proportional to the degree of hypotonicity of the extracellular medium, and there was a close relationship between intracellular Ca concentration and the volumetric changes of the cells. Notably, this response was mediated by Ca -induced Ca release, which is triggered by Ca influx via stretch-activated TRPM7 channels. Furthermore, we detected the generation of Cl currents in the swelling acinar cells upon hypotonic stress, and the current profile matched that of the Ca -activated Cl currents. A specific inhibitor of Cl currents also inhibited the RVD response. In conclusion, an intracellular Ca increase in response to osmotically induced cell swelling plays a critical role in RVD in salivary gland acinar cells.
[Mh] Termos MeSH primário: Células Acinares/metabolismo
Cálcio/metabolismo
Glândulas Salivares/metabolismo
Canais de Cátion TRPM/fisiologia
[Mh] Termos MeSH secundário: Animais
Soluções Hipotônicas
Mecanotransdução Celular
Concentração Osmolar
Técnicas de Patch-Clamp
Plasmídeos
Ratos
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hypotonic Solutions); 0 (TRPM Cation Channels); EC 2.7.11.1 (Trpm7 protein, rat); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:170513
[St] Status:MEDLINE
[do] DOI:10.1177/0022034517708766


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[PMID]:28445759
[Au] Autor:Chabanon M; Ho JCS; Liedberg B; Parikh AN; Rangamani P
[Ad] Endereço:Department of Mechanical and Aerospace Engineering, University of California San Diego, La Jolla, California.
[Ti] Título:Pulsatile Lipid Vesicles under Osmotic Stress.
[So] Source:Biophys J;112(8):1682-1691, 2017 Apr 25.
[Is] ISSN:1542-0086
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The response of lipid bilayers to osmotic stress is an important part of cellular function. Recent experimental studies showed that when cell-sized giant unilamellar vesicles (GUVs) are exposed to hypotonic media, they respond to the osmotic assault by undergoing a cyclical sequence of swelling and bursting events, coupled to the membrane's compositional degrees of freedom. Here, we establish a fundamental and quantitative understanding of the essential pulsatile behavior of GUVs under hypotonic conditions by advancing a comprehensive theoretical model of vesicle dynamics. The model quantitatively captures the experimentally measured swell-burst parameters for single-component GUVs, and reveals that thermal fluctuations enable rate-dependent pore nucleation, driving the dynamics of the swell-burst cycles. We further extract constitutional scaling relationships between the pulsatile dynamics and GUV properties over multiple timescales. Our findings provide a fundamental framework that has the potential to guide future investigations on the nonequilibrium dynamics of vesicles under osmotic stress.
[Mh] Termos MeSH primário: Pressão Osmótica
Estresse Fisiológico
Lipossomas Unilamelares/química
[Mh] Termos MeSH secundário: Dermoscopia
Difusão
Soluções Hipotônicas/química
Bicamadas Lipídicas/química
Modelos Biológicos
Fosfatidilcolinas/química
Sacarose/química
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hypotonic Solutions); 0 (Lipid Bilayers); 0 (Phosphatidylcholines); 0 (Unilamellar Liposomes); 57-50-1 (Sucrose); TE895536Y5 (1-palmitoyl-2-oleoylphosphatidylcholine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170427
[St] Status:MEDLINE


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[PMID]:28408388
[Au] Autor:Shein SL; Slain K; Martinez Schlurmann N; Speicher R; Rotta AT
[Ad] Endereço:Division of Pediatric Critical Care, Rainbow Babies & Children's Hospital, Cleveland, Ohio steven.shein@uhhospitals.org.
[Ti] Título:Hyponatremia and Hypotonic Intravenous Fluids Are Associated With Unfavorable Outcomes of Bronchiolitis Admissions.
[So] Source:Hosp Pediatr;7(5):263-270, 2017 05.
[Is] ISSN:2154-1663
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: Hyponatremia has been associated with unfavorable outcomes when present at admission in children with bronchiolitis. Delayed hyponatremia may be a modifiable risk factor for severe disease that is influenced by intravenous fluid (IVF) tonicity. We hypothesized that both hyponatremia and prescription of severely hypotonic IVF are associated with unfavorable outcomes, and that prescription of severely hypotonic IVF is associated with subsequent hyponatremia. METHODS: Data were retrospectively extracted for 1557 pediatric inpatients with bronchiolitis. Any day on which a subject was prescribed IVF with sodium <70 mEq/L was termed "IVF <70." All other days on which IVF was prescribed were termed "IVF ≥70." Any blood sodium ≤135 mEq/L defined hyponatremia for that day. All other days with sodium available were labeled normonatremia. Variables were compared with Spearman correlation, Wilcoxon rank test, or χ . Significant results had < .05. RESULTS: Blood sodium levels correlated negatively with hospital length of stay ( = -0.477, < .0001). On each of the first 4 days of hospitalization, significantly increased hospital length of stay was observed in patients with hyponatremia ( = 134 [25.7% of subjects with available sodium data]) versus patients with normonatremia ( = 387 [74.3%]), and in patients prescribed IVF <70 ( = 348 [46.3% of subjects prescribed IVF]) versus patients prescribed IVF ≥70 ( = 403 [53.7%]). Patients prescribed IVF <70 had increased rates of hyponatremia on the subsequent day versus patients prescribed IVF ≥70 (50.0% vs 26.9%, < .001). CONCLUSIONS: In children hospitalized with bronchiolitis, hyponatremia may be a modifiable risk factor for severe disease that may be mitigated by avoiding use of severely hypotonic IVF.
[Mh] Termos MeSH primário: Bronquiolite/terapia
Hidratação
Hiponatremia/complicações
Soluções Hipotônicas/efeitos adversos
Tempo de Internação/estatística & dados numéricos
Respiração Artificial/estatística & dados numéricos
[Mh] Termos MeSH secundário: Feminino
Hospitalização
Seres Humanos
Lactente
Infusões Intravenosas
Masculino
Estudos Retrospectivos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hypotonic Solutions)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170415
[St] Status:MEDLINE
[do] DOI:10.1542/hpeds.2016-0205


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[PMID]:28320625
[Au] Autor:Ismail T; Bürgin J; Todorov A; Osinga R; Menzi N; Largo RD; Haug M; Martin I; Scherberich A; Schaefer DJ
[Ad] Endereço:Department of Plastic, Reconstructive, Aesthetic and Hand Surgery, University Hospital of Basel, Basel, BS, Switzerland; Tissue Engineering, Institute for Surgical Research and Hospital Management, University Hospital of Basel, Basel, BS, Switzerland. Electronic address: Tarek.Ismail@usb.ch.
[Ti] Título:Low osmolality and shear stress during liposuction impair cell viability in autologous fat grafting.
[So] Source:J Plast Reconstr Aesthet Surg;70(5):596-605, 2017 May.
[Is] ISSN:1878-0539
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Liposuction and subsequent autologous fat grafting have become essential techniques for fat augmentation in plastic surgery. However, standard harvesting techniques that ensure the survival of adipocytes and stromal vascular fraction (SVF) cells and thus preserve the transplanted fat volume are lacking. In particular, the effect of different parameters of the tumescent solution has not been studied in this context. We hypothesized that the osmolality of the tumescent solution could have a significant effect on the survival of adipocytes and SVF cells. METHODS: We developed two distinct in vitro models based on freshly harvested excision fat from patients undergoing surgical treatment. First, we investigated the effect of osmolality by incubating excision fat in different tumescent solutions and analyzed the total cell survival and the differentiation potential of SVF cells. Vital whole-mount staining, isolation yield of SVF cells, clonogenicity, and osteogenic and adipogenic differentiation capacities were analyzed. Second, we addressed the additional effect of mechanical stress by simulating a liposuction on pieces of excision fat after incubation with the tumescent solutions. RESULTS: Osmolality of the tumescent solution by itself did not have a significant effect on adipocyte and SVF viability or SVF differentiation. However, when osmolality was combined with liposuction, a significant trend toward lower viability and more lipid droplets with lower osmolality was observed. Especially, SVF viability was significantly lower after liposuction with a hypotonic (150 mOsm/kg) solution. CONCLUSION: This study demonstrates the considerable effect of osmolality during liposuction and may lead to the development of "cell-protective" tumescent solutions.
[Mh] Termos MeSH primário: Lipectomia/métodos
Coleta de Tecidos e Órgãos/métodos
[Mh] Termos MeSH secundário: Adipócitos/efeitos dos fármacos
Adipócitos/fisiologia
Adipócitos/transplante
Tecido Adiposo/transplante
Análise de Variância
Diferenciação Celular
Sobrevivência Celular/fisiologia
Células Cultivadas
Feminino
Seres Humanos
Concentração de Íons de Hidrogênio
Soluções Hipertônicas/química
Soluções Hipertônicas/farmacologia
Soluções Hipotônicas/química
Soluções Hipotônicas/farmacologia
Soluções Isotônicas/química
Soluções Isotônicas/farmacologia
Meia-Idade
Concentração Osmolar
Estresse Mecânico
Estresse Fisiológico/fisiologia
Células Estromais/fisiologia
Transplante Autólogo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hypertonic Solutions); 0 (Hypotonic Solutions); 0 (Isotonic Solutions)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170428
[Lr] Data última revisão:
170428
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE


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[PMID]:28274876
[Au] Autor:Hu F; Hui Z; Wei W; Yang J; Chen Z; Guo B; Xing F; Zhang X; Pan L; Xu J
[Ad] Endereço:The Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, TEDA Institute of Applied Physics, School of Physics, Nankai University, Tianjin, China.
[Ti] Título:Hypotonic stress promotes ATP release, reactive oxygen species production and cell proliferation via TRPV4 activation in rheumatoid arthritis rat synovial fibroblasts.
[So] Source:Biochem Biophys Res Commun;486(1):108-115, 2017 Apr 22.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Rheumatoid arthritis (RA) is a chronic and systemic autoimmune-disease with complex and unclear etiology. Hypotonicity of synovial fluid is a typical characteristic of RA, which may play pivotal roles in RA pathogenesis. In this work, we studied the responses of RA synovial fibroblasts to hypotonic stress in vitro and further explored the underlying mechanisms. Data showed that hyposmotic solutions significantly triggered increases in cytosolic calcium concentration ([Ca ] ) of synoviocytes. Subsequently, it caused rapid release of ATP, as well as remarkable production of intracellular reactive oxygen species (ROS). Meanwhile, hypotonic stimulus promoted the proliferation of synovial fibroblasts. These effects were almost abolished by calcium-free buffer and significantly inhibited by gadolinium (III) chloride (a mechanosensitive Ca channel blocker) and ruthenium red (a transient receptor potential vanilloid 4 (TRPV4) blocker). 4α-phorbol 12,13-didecanoate, a specific agonist of TRPV4, also mimicked hypotonic shock-induced responses shown above. In contrast, voltage-gated channel inhibitors verapamil and nifedipine had little influences on these responses. Furthermore, RT-PCR and western blotting evidently detected TRPV4 expression at mRNA and protein level in isolated synoviocytes. Taken together, our results indicated that hypotonic stimulus resulted in ATP release, ROS production, and cell proliferation depending on Ca entry through activation of TRPV4 channel in synoviocytes.
[Mh] Termos MeSH primário: Trifosfato de Adenosina/secreção
Proliferação Celular/efeitos dos fármacos
Fibroblastos/efeitos dos fármacos
Soluções Hipotônicas/farmacologia
Espécies Reativas de Oxigênio/metabolismo
Canais de Cátion TRPV/metabolismo
[Mh] Termos MeSH secundário: Animais
Artrite Experimental/patologia
Artrite Reumatoide/patologia
Western Blotting
Cálcio/metabolismo
Células Cultivadas
Fibroblastos/metabolismo
Expressão Gênica/efeitos dos fármacos
Masculino
Pressão Osmótica
Ratos Wistar
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Membrana Sinovial/patologia
Canais de Cátion TRPV/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hypotonic Solutions); 0 (Reactive Oxygen Species); 0 (TRPV Cation Channels); 0 (Trpv4 protein, rat); 8L70Q75FXE (Adenosine Triphosphate); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170619
[Lr] Data última revisão:
170619
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170310
[St] Status:MEDLINE


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[PMID]:28235528
[Au] Autor:Anta D; Beleña JM; Álvarez R; Núñez M
[Ad] Endereço:Sureste University Hospital, Ronda del Sur 10, Arganda del Rey, Madrid. Electronic address: diego.anta@salud.madrid.es.
[Ti] Título:Effects of pneumoperitoneum on severe hypernatremia in an adult patient who underwent laparoscopic surgery of hydatid cysts.
[So] Source:J Clin Anesth;37:52-54, 2017 Feb.
[Is] ISSN:1873-4529
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We describe the first case of severe hypernatremia associated to laparoscopic surgery for hydatid cyst in an adult patient after the use of hypertonic saline solution with complete resolution. Severe hypernatremia is an unusual fact at the immediate postoperative period but may have fatal consequences for the patient and need immediate action. The patient reached a serum sodium concentration of 179 mmol/L without adverse effects after 6 days of treatment. Laparoscopy could play a crucial role in Na absorption due to high intraabdominal pressure caused by the pneumoperitoneum and its limitations to avoid peritoneal absorption of hypertonic saline solution. The relation between this surgical technique and the severe complication is discussed. More experience is needed in terms of safety for the patient.
[Mh] Termos MeSH primário: Colecistectomia Laparoscópica/efeitos adversos
Equinococose Hepática/cirurgia
Equinococose/cirurgia
Hipernatremia/complicações
Pneumoperitônio Artificial/efeitos adversos
Solução Salina Hipertônica/efeitos adversos
[Mh] Termos MeSH secundário: Analgésicos Opioides/administração & dosagem
Analgésicos Opioides/uso terapêutico
Antibioticoprofilaxia/métodos
Equinococose/diagnóstico por imagem
Equinococose Hepática/diagnóstico por imagem
Efedrina/administração & dosagem
Efedrina/uso terapêutico
Feminino
Fentanila/administração & dosagem
Fentanila/uso terapêutico
Glucose/administração & dosagem
Glucose/uso terapêutico
Seres Humanos
Hidrocortisona/administração & dosagem
Hidrocortisona/uso terapêutico
Hiperglicemia/sangue
Hiperglicemia/tratamento farmacológico
Hipernatremia/sangue
Hipernatremia/tratamento farmacológico
Hipoglicemiantes/administração & dosagem
Hipoglicemiantes/uso terapêutico
Soluções Hipotônicas/administração & dosagem
Soluções Hipotônicas/uso terapêutico
Infusões Intravenosas
Insulina/administração & dosagem
Insulina/uso terapêutico
Imagem por Ressonância Magnética
Meia-Idade
Hipotonia Muscular/tratamento farmacológico
Hipotonia Muscular/etiologia
Dor Pós-Operatória/tratamento farmacológico
Período Pós-Operatório
Solução Salina Hipertônica/administração & dosagem
Sódio/administração & dosagem
Sódio/uso terapêutico
Vasoconstritores/administração & dosagem
Vasoconstritores/uso terapêutico
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Analgesics, Opioid); 0 (Hypoglycemic Agents); 0 (Hypotonic Solutions); 0 (Insulin); 0 (Saline Solution, Hypertonic); 0 (Vasoconstrictor Agents); 9NEZ333N27 (Sodium); GN83C131XS (Ephedrine); IY9XDZ35W2 (Glucose); UF599785JZ (Fentanyl); WI4X0X7BPJ (Hydrocortisone)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170914
[Lr] Data última revisão:
170914
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170226
[St] Status:MEDLINE


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[PMID]:28222129
[Au] Autor:Tejada MA; Hashem N; Calloe K; Klaerke DA
[Ad] Endereço:Department of Physiology, IKVH, Faculty of Health and Medical Sciences, University of Copenhagen, Dyrlaegevej, Frederiksberg C, Denmark.
[Ti] Título:Heteromeric Slick/Slack K+ channels show graded sensitivity to cell volume changes.
[So] Source:PLoS One;12(2):e0169914, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Slick and Slack high-conductance K+ channels are found in the CNS, kidneys, pancreas, among other organs, where they play an important role in cell excitability as well as in ion transport processes. They are both activated by Na+ and Cl- but show a differential regulation by cell volume changes. Slick has been shown to be regulated by cell volume changes, whereas Slack is insensitive. α-subunits of these channels form homomeric as well as heteromeric channels. It is the aim of this work to explore whether the subunit composition of the Slick/Slack heteromeric channel affects the response to osmotic challenges. In order to provide with the adequate water permeability to the cell membrane of Xenopus laevis oocytes, mRNA of aquaporin 1 was co-expressed with homomeric or heteromeric Slick and Slack α-subunits. Oocytes were superfused with hypotonic or hypertonic buffers and changes in currents were measured by two-electrode voltage clamp. This work presents the first heteromeric K+ channel with a characteristic graded sensitivity to small and fast changes in cell volume. Our results show that the cell volume sensitivity of Slick/Slack heteromeric channels is dependent on the number of volume sensitive Slick α-subunits in the tetrameric channels, giving rise to graded cell volume sensitivity. Regulation of the subunit composition of a channel may constitute a novel mechanism to determine volume sensitivity of cells.
[Mh] Termos MeSH primário: Tamanho Celular
Proteínas do Tecido Nervoso/química
Canais de Potássio/química
[Mh] Termos MeSH secundário: Animais
Aquaporina 1/metabolismo
Seres Humanos
Soluções Hipertônicas/farmacologia
Soluções Hipotônicas/farmacologia
Potenciais da Membrana/fisiologia
Proteínas do Tecido Nervoso/metabolismo
Oócitos
Concentração Osmolar
Técnicas de Patch-Clamp
Canais de Potássio/metabolismo
Multimerização Proteica
Subunidades Proteicas
RNA Mensageiro/genética
Ratos
Proteínas Recombinantes de Fusão/metabolismo
Xenopus laevis
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hypertonic Solutions); 0 (Hypotonic Solutions); 0 (KCNT2 protein, human); 0 (Nerve Tissue Proteins); 0 (Potassium Channels); 0 (Protein Subunits); 0 (RNA, Messenger); 0 (Recombinant Fusion Proteins); 0 (Slack protein, rat); 146410-94-8 (Aquaporin 1)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170222
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0169914


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[PMID]:28185236
[Au] Autor:Shiozaki A; Ichikawa D; Kosuga T; Marunaka Y; Otsuji E
[Ad] Endereço:Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, Kyoto, 602-8566, Japan. shiozaki@koto.kpu-m.ac.jp.
[Ti] Título:Regulation of osmolality for cancer treatment.
[So] Source:J Physiol Sci;67(3):353-360, 2017 May.
[Is] ISSN:1880-6562
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Disseminated metastasis is associated with a poor prognosis, and its management in the peritoneal or pleural cavity is crucial in the treatment of cancer. Recent studies show that ion and water transporters play important roles in fundamental cellular functions, including the regulation of cell volume that would be involved in the cancer process. Here, we review the evidence for hypotonic treatments of cancer and evaluate the potential of the cellular physiological approach in clinical management. The regulation of extracellular osmolality is a promising method, with several studies demonstrating the cytocidal effects of hypotonic solution on cancer cells. Peritoneal lavage with distilled water (DW) during surgery is reported to improve the survival rate of patients with spontaneously ruptured hepatocellular carcinoma. The in vitro studies included in this review also indicate the cytocidal effects of hypotonic shock on esophageal, gastric, colonic, pancreatic, and liver cancer cells with several unique methods and apparatuses, such as a differential interference contrast microscope connected to a digital video camera, a high-resolution flow cytometer and re-incubation analysis. The in vivo studies demonstrate the safeness of a peritoneal injection of DW into mice and indicate that the development of dissemination nodules can be prevented by the pre-incubation of cancer cells with DW or the peritoneal injection of DW. We also demonstrate that the blockade of Cl channels/transporters enhances the cytocidal effects of hypotonic shock by inhibiting regulatory volume decrease in various cancer cells. A deeper understanding of molecular mechanisms may lead to the discovery of these cellular physiological approaches as a novel therapeutic strategy for disseminated metastasis.
[Mh] Termos MeSH primário: Neoplasias/fisiopatologia
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Soluções Hipotônicas/administração & dosagem
Metástase Neoplásica/fisiopatologia
Concentração Osmolar
Pressão Osmótica/fisiologia
Lavagem Peritoneal/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Hypotonic Solutions)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE
[do] DOI:10.1007/s12576-017-0528-x


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[PMID]:28143710
[Au] Autor:Mai TH; Garland EM; Diedrich A; Robertson D
[Ad] Endereço:Department of Pharmacology, Vanderbilt University, Nashville, TN, United States; Department of Clinical Pharmacology, Vanderbilt University, Nashville, TN, United States.
[Ti] Título:Hepatic and renal mechanisms underlying the osmopressor response.
[So] Source:Auton Neurosci;203:58-66, 2017 Mar.
[Is] ISSN:1872-7484
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Increased blood pressure (BP) is observed in patients with impaired baroreflexes after water drinking. The stimulus for this effect is low blood osmolality, and it has been termed the osmopressor response (OPR). The BP increase is associated with activation of the sympathetic nervous system and a requirement for transient receptor potential vanilloid 4 (TRPV4) channels. However, the mechanisms underlying the OPR are poorly understood. We tested the hypothesis that hypotonicity is sensed in the portal area to initiate the OPR. Sino-aortic denervated mice were used and BP was monitored for 30min after fluid infusion while mice were under anesthesia. Infusion of hypotonic fluid (0.45% saline), but not of isotonic 0.9% saline, directly into the portal vein, produced an immediate OPR (increase in BP with saline 0.45%: 15±13 vs. 0.9%: -7±2mmHg, p=0.003; AUC: 0.45%: 150±99, n=7 vs. 0.9%: -74±60mmHg·min, n=5, p=0.003). However, 0.45% saline was not able to trigger a similar response in TRPV4 mice (ΔBP : -2±5mmHg, n=8, p=0.009). Hypotonic saline did not raise BP when infused at the same speed and volume into the jugular vein (jugular: -5±6mmHg, p=0.002, compared to portal). Denervation of the splanchnic nerve by celiac ganglionectomy (CGX) did not abolish the OPR (CGX: 15±11 vs. Sham: 16±6mmHg, p=0.34). Renal denervation diminished the OPR elicited by duodenal water infusion (denervation: 9±4 vs. sham: 31±15mmHg, p=0.016). Therefore, hypotonicity in the portal circulation, probably sensed by TRPV4 channels, triggers the OPR and intact renal nerves are needed for the full response.
[Mh] Termos MeSH primário: Barorreflexo/fisiologia
Pressão Sanguínea/fisiologia
Ingestão de Líquidos/fisiologia
Rim/metabolismo
Fígado/metabolismo
[Mh] Termos MeSH secundário: Animais
Denervação Autônoma
Água Potável/administração & dosagem
Duodeno/metabolismo
Soluções Hipotônicas/administração & dosagem
Veias Jugulares/metabolismo
Masculino
Camundongos Endogâmicos C57BL
Camundongos Knockout
Modelos Animais
Concentração Osmolar
Veia Porta/metabolismo
Cloreto de Sódio na Dieta/administração & dosagem
Nervos Esplâncnicos/metabolismo
Canais de Cátion TRPV/genética
Canais de Cátion TRPV/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drinking Water); 0 (Hypotonic Solutions); 0 (Sodium Chloride, Dietary); 0 (TRPV Cation Channels); 0 (Trpv4 protein, mouse)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170202
[St] Status:MEDLINE



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