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[PMID]:29329303
[Au] Autor:Sienkiewicz W; Dudek A; Czaja K; Janeczek M; Chrószcz A; Kaleczyc J
[Ad] Endereço:Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland.
[Ti] Título:Efficacy of lateral- versus medial-approach hip joint capsule denervation as surgical treatments of the hip joint pain; a neuronal tract tracing study in the sheep.
[So] Source:PLoS One;13(1):e0190052, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To evaluate efficacy of denervation of the of the hip joint capsule (HJC), as a treatment of hip joint pain. Specifically, we tested the hypothesis that HJC denervation will significantly reduce the number of sensory neurons innervating the capsule. STUDY DESIGN: Denervation of the HJC from a medial or lateral approach was followed by retrograde tracing of sensory neurons innervating the capsule. ANIMALS: Twenty adult male sheep (30-40 kg of body weight; Polish merino breed) were used in the study. METHODS: The hip joint was denervated from medial (n = 5) or lateral (n = 5) surgical approaches. Immediately after denervation, the retrograde neural tract tracer Fast Blue (FB) was injected into the HJC. An additional ten animals (n = 5 for medial and n = 5 for lateral approach) received the same treatment without HJC denervation to provide the appropriate controls. RESULTS: Results of the study revealed that the vast majority of retrogradely labelled sensory neurons innervating the HJC originate from fifth lumbar to second sacral dorsal root ganglia. Both the medial and the lateral denervations significantly reduced the number of sensory neurons innervating the HJC (39.2% and 69.0% reduction respectively). CONCLUSIONS: These results show that denervation of the HJC is an effective surgical procedure for reduction of the sensory neuronal input to the HJC. Moreover, the lateral approach was found to be significantly more effective for reducing sensory innervation as compared to the medial one.
[Mh] Termos MeSH primário: Articulação do Quadril/fisiologia
Marcadores do Trato Nervoso
[Mh] Termos MeSH secundário: Animais
Articulação do Quadril/inervação
Masculino
Ovinos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Neuronal Tract-Tracers)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190052


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[PMID]:28542213
[Au] Autor:Gross C; Ellison B; Buchman AS; Terasawa E; VanderHorst VG
[Ad] Endereço:Department of Neurology, Division of Movement Disorders, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, United States of America.
[Ti] Título:A novel approach for assigning levels to monkey and human lumbosacral spinal cord based on ventral horn morphology.
[So] Source:PLoS One;12(5):e0177243, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Proper identification of spinal cord levels is crucial for clinical-pathological and imaging studies in humans, but can be a challenge given technical limitations. We have previously demonstrated in non-primate models that the contours of the spinal ventral horn are determined by the position of motoneuron pools. These positions are preserved within and among individuals and can be used to identify lumbosacral spinal levels. Here we tested the hypothesis that this approach can be extended to identify monkey and human spinal levels. In 7 rhesus monkeys, we retrogradely labeled motoneuron pools that represent rostral, middle and caudal landmarks of the lumbosacral enlargement. We then aligned the lumbosacral enlargements among animals using absolute length, segmental level or a relative scale based upon rostral and caudal landmarks. Inter-animal matching of labeled motoneurons across the lumbosacral enlargement was most precise when using internal landmarks. We then reconstructed 3 human lumbosacral spinal cords, and aligned these based upon homologous internal landmarks. Changes in shape of the ventral horn were consistent among human subjects using this relative scale, despite marked differences in absolute length or age. These data suggest that the relative position of spinal motoneuron pools is conserved across species, including primates. Therefore, in clinical-pathological or imaging studies in humans, one can assign spinal cord levels to even single sections by matching ventral horn shape to standardized series.
[Mh] Termos MeSH primário: Células do Corno Anterior/citologia
Região Lombossacral/anatomia & histologia
[Mh] Termos MeSH secundário: Idoso de 80 Anos ou mais
Animais
Gatos
Feminino
Fixadores
Formaldeído
Seres Humanos
Macaca mulatta
Masculino
Meia-Idade
Técnicas de Rastreamento Neuroanatômico
Marcadores do Trato Nervoso
Especificidade da Espécie
Fixação de Tecidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fixatives); 0 (Neuronal Tract-Tracers); 1HG84L3525 (Formaldehyde)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177243


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[PMID]:28083561
[Au] Autor:Iyilikci O; Balthazart J; Ball GF
[Ad] Endereço:Department of Psychological and Brain Sciences, Johns Hopkins University, Baltimore, Maryland 21218; Department of Psychology, University of Maryland, College Park, College Park, Maryland 20742.
[Ti] Título:Medial Preoptic Regulation of the Ventral Tegmental Area Related to the Control of Sociosexual Behaviors.
[So] Source:eNeuro;3(6), 2016 Nov-Dec.
[Is] ISSN:2373-2822
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:During sociosexual encounters, different brain mechanisms interact to orchestrate information about the salience of external stimuli along with the current physiological and environmental conditions in order to process these in an optimal manner. One candidate neural system involves the potential interplay between the medial preoptic nucleus (POM) and mesolimbic reward circuitry. We present here evidence indicating that projections originating from the POM play a modulatory role on the mesolimbic reward circuitry related to male sexual behavior in Japanese quail ( ). First, we used an asymmetrical inactivation strategy where POM and ventral tegmental area (VTA) were unilaterally inactivated via the GABA agonist muscimol, either in an ipsilateral or contralateral fashion. Ipsilateral injections of muscimol had negligible effects on both appetitive and consummatory sexual behaviors. In contrast, contralateral injections significantly impaired appetitive sexual behaviors but had no clear effect on consummatory sexual behaviors. Next, we labeled cells projecting from the POM to the VTA by stereotaxic injection into VTA of the retrograde tracer biotinylated dextran amine (BDA). Two weeks later, brains from males who had been allowed to interact freely with a female (15 min) or kept as controls were collected and fixed for double immunohistochemical labeling of BDA and the immediate early gene Fos. More retrogradely labeled BDA cells in POM expressed Fos after sociosexual interactions than in control conditions. Overall, these findings provide novel evidence for the interplay between POM and VTA in the modulation of appetitive but not consummatory sexual behaviors. Schematic representation of the putative role of the projection from the medial POM to the VTA in the regulation of appetitive and consummatory sexual behaviors. Unilateral inactivation of POM and VTA on (1) ipsilateral sides has negligible effects on both aspects of sexual behaviors, whereas (2) contralateral inactivation disrupts appetitive sexual behaviors.
[Mh] Termos MeSH primário: Área Pré-Óptica/fisiologia
Comportamento Sexual Animal/fisiologia
Área Tegmentar Ventral/fisiologia
[Mh] Termos MeSH secundário: Animais
Comportamento Apetitivo/fisiologia
Proteínas Aviárias/antagonistas & inibidores
Proteínas Aviárias/metabolismo
Biotina/análogos & derivados
Cateteres de Demora
Coturnix
Dextranos
Lateralidade Funcional
Agonistas de Receptores de GABA-A/farmacologia
Imuno-Histoquímica
Masculino
Muscimol/farmacologia
Vias Neurais/citologia
Vias Neurais/efeitos dos fármacos
Vias Neurais/fisiologia
Técnicas de Rastreamento Neuroanatômico
Marcadores do Trato Nervoso
Área Pré-Óptica/citologia
Área Pré-Óptica/efeitos dos fármacos
Proteínas Proto-Oncogênicas c-fos/metabolismo
Receptores de GABA-A/metabolismo
Comportamento Sexual Animal/efeitos dos fármacos
Comportamento Social
Área Tegmentar Ventral/citologia
Área Tegmentar Ventral/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Avian Proteins); 0 (Dextrans); 0 (GABA-A Receptor Agonists); 0 (Neuronal Tract-Tracers); 0 (Proto-Oncogene Proteins c-fos); 0 (Receptors, GABA-A); 0 (biotinylated dextran amine); 2763-96-4 (Muscimol); 6SO6U10H04 (Biotin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170114
[St] Status:MEDLINE


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[PMID]:27617735
[Au] Autor:Fox ME; Bucher ES; Johnson JA; Wightman RM
[Ad] Endereço:Department of Chemistry, Neuroscience Center, University of North Carolina at Chapel Hill , Chapel Hill, North Carolina 27599-3290, United States.
[Ti] Título:Medullary Norepinephrine Projections Release Norepinephrine into the Contralateral Bed Nucleus of the Stria Terminalis.
[So] Source:ACS Chem Neurosci;7(12):1681-1689, 2016 Dec 21.
[Is] ISSN:1948-7193
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Central norepinephrine signaling influences a wide range of behavioral and physiological processes, and the ventral bed nucleus of the stria terminalis (vBNST) receives some of the densest norepinephrine innervation in the brain. Previous work describes norepinephrine neurons as projecting primarily unilaterally; however, recent evidence for cross-hemispheric catecholamine signaling challenges this idea. Here, we use fast-scan cyclic voltammetry and retrograde tracing to characterize cross-hemispheric norepinephrine signaling in the vBNST. We delivered stimulations to noradrenergic pathways originating in the A1/A2 and locus coeruleus and found hemispherically equivalent norepinephrine release in the vBNST regardless of stimulated hemisphere. Unilateral retrograde tracing revealed that medullary, but not locus coeruleus norepinephrine neurons send cross-hemispheric projections to the vBNST. Further characterization with pharmacological lesions revealed that stimulations of the locus coeruleus and its axon bundles likely elicit vBNST norepinephrine release through indirect activation. These experiments are the first to demonstrate contralateral norepinephrine release and establish that medullary, but not coerulean neurons are responsible for norepinephrine release in the vBNST.
[Mh] Termos MeSH primário: Lateralidade Funcional
Bulbo/metabolismo
Neurônios/metabolismo
Norepinefrina/metabolismo
Núcleos Septais/metabolismo
[Mh] Termos MeSH secundário: Animais
Estimulação Elétrica
Lateralidade Funcional/fisiologia
Ácido Ibotênico
Locus Cerúleo/citologia
Locus Cerúleo/lesões
Locus Cerúleo/metabolismo
Masculino
Bulbo/citologia
Vias Neurais/citologia
Vias Neurais/metabolismo
Técnicas de Rastreamento Neuroanatômico
Marcadores do Trato Nervoso
Neurônios/citologia
Oxidopamina
Ratos Sprague-Dawley
Núcleos Septais/citologia
Estilbamidinas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt); 0 (Neuronal Tract-Tracers); 0 (Stilbamidines); 2552-55-8 (Ibotenic Acid); 8HW4YBZ748 (Oxidopamine); X4W3ENH1CV (Norepinephrine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160913
[St] Status:MEDLINE
[do] DOI:10.1021/acschemneuro.6b00210


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[PMID]:27575005
[Au] Autor:Kuroiwa M; Fukushima N; Yokouchi K; Kawagishi K; Moriizumi T
[Ad] Endereço:Department of Neurosurgery, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan.
[Ti] Título:Morphological analysis of regenerated bulbar fibers in relation to neonatal olfaction.
[So] Source:Brain Res Bull;127:66-73, 2016 Oct.
[Is] ISSN:1873-2747
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:It was revealed that regeneration of the lateral olfactory tract (LOT) occurred in developing rats and the regenerated olfactory system was functional 4 weeks after transection. The aim of this study was to determine the earliest onset of functional recovery in LOT-injured rats and to quantify regenerated nerve components with functional correlation. Neonatal rats on postnatal day (P) 2 were subjected to unilateral transection of the left LOT and underwent unilateral removal of the right olfactory bulb on P11. Functional recovery of the tract injury was assessed by the suckling capability, which can be achieved by olfaction. Suckling capability was observed on P12 in most neonatally LOT-transected pups. Rat pups were subjected to unilateral transection of the left LOT on P2, and received injections of biotinylated dextran amine (BDA) into the bilateral olfactory bulb on P5 to quantify normal and regenerated nerve components in the olfactory cortices at the level of the olfactory tubercle. BDA(+) areas and density indices of the olfactory cortices in the neonatally LOT-transected P12 pups were 11.05×10 µm and 0.35 on the normal right side and 4.34×10 µm and 0.21 on the transected left side. We concluded that functional recovery of the LOT-transected neonatal rats occurred as early as 10days after tract transection and that areas and densities of regenerated nerve components essential for functional recovery were approximately 40% and 60% of the age-matched normal values in the olfactory cortices at the level of the olfactory tubercle.
[Mh] Termos MeSH primário: Regeneração Nervosa/fisiologia
Bulbo Olfatório/patologia
Condutos Olfatórios/lesões
Condutos Olfatórios/patologia
Recuperação de Função Fisiológica/fisiologia
Olfato/fisiologia
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos
Biotina/análogos & derivados
Dextranos
Corantes Fluorescentes
Marcadores do Trato Nervoso
Bulbo Olfatório/fisiopatologia
Córtex Olfatório/patologia
Córtex Olfatório/fisiopatologia
Condutos Olfatórios/fisiopatologia
Ratos Wistar
Comportamento de Sucção/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dextrans); 0 (Fluorescent Dyes); 0 (Neuronal Tract-Tracers); 0 (biotinylated dextran amine); 6SO6U10H04 (Biotin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160831
[St] Status:MEDLINE


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[PMID]:27436534
[Au] Autor:Ni RJ; Luo PH; Shu YM; Chen JT; Zhou JN
[Ad] Endereço:Chinese Academy of Science Key Laboratory of Brain Function and Diseases, School of Life Sciences, University of Science and Technology of China, Hefei 230027, Anhui, PR China.
[Ti] Título:Whole-brain mapping of afferent projections to the bed nucleus of the stria terminalis in tree shrews.
[So] Source:Neuroscience;333:162-80, 2016 Oct 01.
[Is] ISSN:1873-7544
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The bed nucleus of the stria terminalis (BST) plays an important role in integrating and relaying input information to other brain regions in response to stress. The cytoarchitecture of the BST in tree shrews (Tupaia belangeri chinensis) has been comprehensively described in our previous publications. However, the inputs to the BST have not been described in previous reports. The aim of the present study was to investigate the sources of afferent projections to the BST throughout the brain of tree shrews using the retrograde tracer Fluoro-Gold (FG). The present results provide the first detailed whole-brain mapping of BST-projecting neurons in the tree shrew brain. The BST was densely innervated by the prefrontal cortex, entorhinal cortex, ventral subiculum, amygdala, ventral tegmental area, and parabrachial nucleus. Moreover, moderate projections to the BST originated from the medial preoptic area, supramammillary nucleus, paraventricular thalamic nucleus, pedunculopontine tegmental nucleus, dorsal raphe nucleus, locus coeruleus, and nucleus of the solitary tract. Afferent projections to the BST are identified in the ventral pallidum, nucleus of the diagonal band, ventral posteromedial thalamic nucleus, posterior complex of the thalamus, interfascicular nucleus, retrorubral field, rhabdoid nucleus, intermediate reticular nucleus, and parvicellular reticular nucleus. In addition, the different densities of BST-projecting neurons in various regions were analyzed in the tree shrew brains. In summary, whole-brain mapping of direct inputs to the BST is delineated in tree shrews. These brain circuits are implicated in the regulation of numerous physiological and behavioral processes including stress, reward, food intake, and arousal.
[Mh] Termos MeSH primário: Núcleos Septais/anatomia & histologia
Tupaiidae/anatomia & histologia
[Mh] Termos MeSH secundário: Vias Aferentes/anatomia & histologia
Animais
Imuno-Histoquímica
Masculino
Técnicas de Rastreamento Neuroanatômico
Marcadores do Trato Nervoso
Fotomicrografia
Estilbamidinas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt); 0 (Neuronal Tract-Tracers); 0 (Stilbamidines)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160721
[St] Status:MEDLINE


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[PMID]:27288218
[Au] Autor:Mondello SE; Jefferson SC; O'Steen WA; Howland DR
[Ad] Endereço:Department of Rehabilitation Medicine, University of Washington, Seattle, WA 98195, United States.
[Ti] Título:Enhancing Fluorogold-based neural tract tracing.
[So] Source:J Neurosci Methods;270:85-91, 2016 09 01.
[Is] ISSN:1872-678X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Fluorogold (FG) is used by many groups to retrogradely trace nervous system pathways. Fluorogold, while a robust tracer, also is neurotoxic and causes tissue damage at the injection site and leads to motor deficits. NEW METHOD: In the current study, we describe a method for enhancing FG-uptake using Triton™ and an overall procedure for reducing FG-related tissue damage while still allowing effective quantification. RESULTS: Triton™ decreases the amount of FG, as well as the time required for long-distance transport from the thoracic spinal cord to the motor cortex by >4 fold when this distance is >10in. Although small FG concentrations and injection volumes are ideal for minimizing associated tissue damage and motor deficits, they result in difficult-to-detect fluorescence. This can be solved using FG antiserum paired with an ABC chromogen reaction. This ABC chromogen reaction product can remain stable for at least 9 years. COMPARISON WITH EXISTING METHOD(S): This study is the first to collectively address FG-induced tissue damage and describe methods for minimizing this damage. CONCLUSIONS: Triton™ enhances the uptake of FG in the nervous system, reduces the FG required, and allows for a substantial decrease in tracing time that limits FG-induced motor deficits. Small FG concentration and volume decreases tissue damage but also decreases FG fluorescent detection. Detection challenges are resolved using FG anti-serum and chromogen reactions.
[Mh] Termos MeSH primário: Netuno
Técnicas de Rastreamento Neuroanatômico
Marcadores do Trato Nervoso
Estilbamidinas
[Mh] Termos MeSH secundário: Animais
Benzoxazinas
Gatos
Feminino
Imuno-Histoquímica
Microscopia de Fluorescência
Córtex Motor/patologia
Necrose/etiologia
Necrose/patologia
Marcadores do Trato Nervoso/efeitos adversos
Medula Espinal/citologia
Medula Espinal/diagnóstico por imagem
Medula Espinal/patologia
Traumatismos da Medula Espinal/patologia
Estilbamidinas/efeitos adversos
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt); 0 (Benzoxazines); 0 (Neuronal Tract-Tracers); 0 (Stilbamidines); 2AB49C465R (cresyl violet)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171127
[Lr] Data última revisão:
171127
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160612
[St] Status:MEDLINE


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[PMID]:27155454
[Au] Autor:Schmued LC
[Ad] Endereço:US Food and Drug Administration (FDA), National Center for Toxicological Research (NCTR), Division of Neurotoxicology, 3900 NCTR Rd, Jefferson, AR 72079United States. Electronic address: larry.schmued@fda.hhs.gov.
[Ti] Título:Development and application of novel histochemical tracers for localizing brain connectivity and pathology.
[So] Source:Brain Res;1645:31-5, 2016 Aug 15.
[Is] ISSN:1872-6240
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:FLUORO-GOLD: A NEW FLUORESCENT RETROGRADE AXONAL TRACER WITH NUMEROUS UNIQUE PROPERTIES: A new fluorescent dye, Fluoro-Gold, has been demonstrated to undergo retrograde axonal transport. Its properties include (1) intense fluorescence, (2) extensive filling of dendrites, (3) high resistance to fading, (4) no uptake by intact undamaged fibers of passage, (5) no diffusion from labeled cells, (6) consistent and pure commercial source, (7) wide latitude of survival times and (8) compatibility with all other tested neuro-histochemical techniques. © 1986. Fluoro-Jade C results in ultra high resolution and contrast labeling of degenerating neurons: The causes and effects of neuronal degeneration are of major interest to a wide variety of neuroscientists. Paralleling this growing interest is an increasing number of methods applicable to the detection of neuronal degeneration. The earliest methods employing aniline dyes were methodologically simple, but difficult to interpret due to a lack of staining specificity. In an attempt to circumvent this problem, numerous suppressed silver methods have been introduced. However, these methods are labor intensive, incompatible with most other histochemical procedures and notoriously capricious. In an attempt to develop a tracer with the methodological simplicity and reliability of conventional stains but with the specificity of an ideal suppressed silver preparation, the Fluoro-Jade dyes were developed. Fluoro-Jade C, like its predecessors, Fluoro-Jade and Fluoro-Jade B, was found to stain all degenerating neurons, regardless of specific insult or mechanism of cell death. Therefore, the patterns of neuronal degeneration seen following exposure to either the glutamate agonist, kainic acid, or the inhibitor of mitochondrial respiration, 3-NPA, were the same for all of the Fluoro-Jade dyes. However, there was a qualitative difference in the staining characteristics of the three fluorochromes. Specifically, Fluoro-Jade C exhibited the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localizing not only degenerating nerve cell bodies, but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols. Triple labeling was accomplished by staining degenerating neurons with Fluoro-Jade C, cell nuclei with DAPI and activated astrocytes with GFAP immunofluoresence. © 2005. ARTICLE ABSTRACT: The development of novel tracers and associated histochemical methods has always been need driven. One such need was the development of tracers that could be administered to discrete brain regions in vivo to subsequently reveal neuronal connectivity via axonal transport of the tracer. One such compound is Fluoro-Gold (F-G), which can be used to demonstrate retrograde axonal transport. Advantages of this fluorescent tracer include brightness, sensitivity, contrast, stability, permanence and compatibility with multiple labeling studies. It may be applied to resolve either the afferent or efferent connections of brain regions of interest. Another need addressed was for a simple and definitive way to localize degenerating neurons in brain tissue sections. This led to the development of Fluoro-Jade B (FJ-B) and Fluoro-Jade C (FJ-C). Advantages of these fluorescent histochemical tracers include high specificity, resolution, contrast, stability and suitability for use in multiple labeling studies. These methods can be applied to detect both apoptotic and necrotic neuronal degeneration following a variety of insults including physical trauma, neurodegenerative disease and a wide variety of neurotoxicants. This article is part of a Special Issue entitled SI:50th Anniversary Issue.
[Mh] Termos MeSH primário: Encéfalo/citologia
Encéfalo/patologia
Técnicas de Rastreamento Neuroanatômico
Marcadores do Trato Nervoso
Neurônios/citologia
Neurônios/patologia
[Mh] Termos MeSH secundário: Animais
Corantes Fluorescentes
Seres Humanos
Vias Neurais/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Fluorescent Dyes); 0 (Neuronal Tract-Tracers)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160508
[St] Status:MEDLINE


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[PMID]:27126450
[Au] Autor:Olsen GM; Witter MP
[Ad] Endereço:Kavli Institute for Systems Neuroscience, Centre for Neural Computation, NTNU Norwegian University of Science and Technology, The Faculty of Medicine, 7491, Trondheim, Norway.
[Ti] Título:Posterior parietal cortex of the rat: Architectural delineation and thalamic differentiation.
[So] Source:J Comp Neurol;524(18):3774-3809, 2016 Dec 15.
[Is] ISSN:1096-9861
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study refines the characterization of the rat parietal cortical domain in terms of cyto- and chemoarchitecture as well as thalamic connectivity. We recognize three subdivisions of the posterior parietal cortex (PPC), which are architectonically distinct from the neighboring somatosensory and visual cortices. Furthermore, we show that the different parietal areas are differently connected with thalamic nuclei. The medial portion of PPC (mPPC) is connected primarily with the medial portion of the lateral posterior nucleus (LP), whereas the lateral portion (lPPC) connects with the posterior complex (Po). The more caudolateral part of PPC (PtP) also projects to Po but can be distinguished from lPPC based on architectonic criteria. The primary somatic and visual cortices, neighboring PPC, are preferentially connected with the primary ventral posterior and dorsolateral geniculate nuclei, respectively, and less with the associational Po and LP. Particularly the border between the secondary visual cortex and the PPC has been a matter of controversy, but here we show that, although PPC subareas are connected with Po and medial LP, the medial and lateral secondary visual cortices are connected with lateral LP and a portion of medial LP different from that connected with PPC. The resulting delineations and specifications of connectivity with thalamic nuclei together with upcoming studies of cortical connectivity will facilitate detailed studies on the role of the subdivisions of PPC in the rat as diverse, higher order associative cortical areas, comparable to those described in the primate.for J. Comp. Neurol. 524:3774-3809, 2016. © 2016 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Lobo Parietal/anatomia & histologia
Ratos Sprague-Dawley/anatomia & histologia
[Mh] Termos MeSH secundário: Animais
Feminino
Masculino
Vias Neurais/anatomia & histologia
Técnicas de Rastreamento Neuroanatômico
Marcadores do Trato Nervoso
Tálamo/anatomia & histologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neuronal Tract-Tracers)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160430
[St] Status:MEDLINE
[do] DOI:10.1002/cne.24032


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[PMID]:27019197
[Au] Autor:Spencer NJ; Kyloh M; Beckett EA; Brookes S; Hibberd T
[Ad] Endereço:Discipline of Human Physiology and Centre for Neuroscience, School of Medicine, Flinders University, Adelaide, 5001, South Australia, Australia.
[Ti] Título:Different types of spinal afferent nerve endings in stomach and esophagus identified by anterograde tracing from dorsal root ganglia.
[So] Source:J Comp Neurol;524(15):3064-83, 2016 Oct 15.
[Is] ISSN:1096-9861
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In visceral organs of mammals, most noxious (painful) stimuli as well as innocuous stimuli are detected by spinal afferent neurons, whose cell bodies lie in dorsal root ganglia (DRGs). One of the major unresolved questions is the location, morphology, and neurochemistry of the nerve endings of spinal afferents that actually detect these stimuli in the viscera. In the upper gastrointestinal (GI) tract, there have been many anterograde tracing studies of vagal afferent endings, but none on spinal afferent endings. Recently, we developed a technique that now provides selective labeling of only spinal afferents. We used this approach to identify spinal afferent nerve endings in the upper GI tract of mice. Animals were anesthetized, and injections of dextran-amine were made into thoracic DRGs (T8-T12). Seven days post surgery, mice were euthanized, and the stomach and esophagus were removed, fixed, and stained for calcitonin gene-related peptide (CGRP). Spinal afferent axons were identified that ramified extensively through many rows of myenteric ganglia and formed nerve endings in discrete anatomical layers. Most commonly, intraganglionic varicose endings (IGVEs) were identified in myenteric ganglia of the stomach and varicose simple-type endings in the circular muscle and mucosa. Less commonly, nerve endings were identified in internodal strands, blood vessels, submucosal ganglia, and longitudinal muscle. In the esophagus, only IGVEs were identified in myenteric ganglia. No intraganglionic lamellar endings (IGLEs) were identified in the stomach or esophagus. We present the first identification of spinal afferent endings in the upper GI tract. Eight distinct types of spinal afferent endings were identified in the stomach, and most of them were CGRP immunoreactive. J. Comp. Neurol. 524:3064-3083, 2016. © 2016 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Esôfago/citologia
Esôfago/inervação
Gânglios Espinais/citologia
Neurônios Aferentes/citologia
Estômago/citologia
Estômago/inervação
[Mh] Termos MeSH secundário: Vias Aferentes/citologia
Animais
Feminino
Imuno-Histoquímica
Masculino
Camundongos Endogâmicos C57BL
Membrana Mucosa/citologia
Membrana Mucosa/inervação
Marcadores do Trato Nervoso
Vértebras Torácicas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neuronal Tract-Tracers)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160329
[St] Status:MEDLINE
[do] DOI:10.1002/cne.24006



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