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[PMID]: | 29175396 |
[Au] Autor: | Wang F; Liu F; Chen W; Xu R; Wang W |
[Ad] Endereço: | School of Biological Science, Luoyang Normal University, Luoyang, 471022, China; Cold Water Fish Breeding Engineering Technology Research Center of Henan Province, Luoyang, 471022, China. Electronic address: wangfan7677@163.com. |
[Ti] Título: | Effects of triclosan (TCS) on hormonal balance and genes of hypothalamus-pituitary- gonad axis of juvenile male Yellow River carp (Cyprinus carpio). |
[So] Source: | Chemosphere;193:695-701, 2018 Feb. | [Is] ISSN: | 1879-1298 |
[Cp] País de publicação: | England |
[La] Idioma: | eng |
[Ab] Resumo: | Triclosan (TCS) is a broad spectrum antimicrobial agent which has been widely dispersed and determinated in the aquatic environment. However, the effects of TCS on reproductive endocrine in male fish are poorly understood. In this study, male Yellow River carp (Cyprinus carpio) were exposed to 0, 1/5, 1/10 and 1/20 LC (96 h LC of TCS to carp) TCS under semi-static conditions for 42 d. Vitellogenin (Vtg), 17ß-estradiol (E ), testosterone(T), gonadotropin (GtH), and gonadotropin-releasing hormone (GnRH) levels were measured by enzyme-linked immunosorbent assay (ELISA). Meanwhile, we also examined the mRNA expressions of aromatase, GtHs-ß, GnRH, estrogen receptor (Er), and androgen receptor (Ar) by quantitative Real-time Polymerase Chain Reaction (qRT-PCR). TCS induced Vtg levels of hepatopancreas, E levels of serum, and inhibited Ar and Er mRNA levels, suggesting that the induction of Vtg production by TCS was indirectly caused by non-Er pathways. TCS-induced Vtg levels by interfering with the reproductive axis at plenty of latent loci of male carps: (a) TCS exposure increased the aromatase mRNA expression of hypothalamus and gonad aromatase, consequently increasing serum concentrations of E to induce Vtg in hepatopancreas; (b) TCS treatment changed GtH-ß and GnRH mRNA expression and secretion, causing the disturbance of reproductive endocrine; (c) TCS exposure decreased Ar mRNA levels, indicating potential Ar-mediated antiandrogen action. These mechanisms showed that TCS may induce Vtg production in male carp by non-Er-mediated pathways. |
[Mh] Termos MeSH primário: |
Carpas/metabolismo Triclosan/toxicidade Poluentes Químicos da Água/toxicidade
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[Mh] Termos MeSH secundário: |
Animais Anti-Infecciosos/toxicidade Aromatase/genética Sistema Endócrino/efeitos dos fármacos Ensaio de Imunoadsorção Enzimática Estradiol/análise Hormônio Liberador de Gonadotropina/análise Hormônio Liberador de Gonadotropina/genética Gônadas/enzimologia Gônadas/metabolismo Hepatopâncreas/metabolismo Hormônios/metabolismo Hipotálamo/metabolismo Masculino Hipófise/metabolismo RNA Mensageiro/análise Reação em Cadeia da Polimerase em Tempo Real Receptores Estrogênicos/genética Reprodução/efeitos dos fármacos Testosterona/análise Vitelogeninas/análise
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Anti-Infective Agents); 0 (Hormones); 0 (RNA, Messenger); 0 (Receptors, Estrogen); 0 (Vitellogenins); 0 (Water Pollutants, Chemical); 33515-09-2 (Gonadotropin-Releasing Hormone); 3XMK78S47O (Testosterone); 4NM5039Y5X (Triclosan); 4TI98Z838E (Estradiol); EC 1.14.14.1 (Aromatase); EC 1.14.14.1 (CYP19A1 protein, human) |
[Em] Mês de entrada: | 1803 |
[Cu] Atualização por classe: | 180309 |
[Lr] Data última revisão:
| 180309 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 171128 |
[St] Status: | MEDLINE |
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