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[PMID]:27865415
[Au] Autor:Roy VK; Peki V; Devi MS; Sanjeev S; Khusboo M; Zothansanga R; Ibrahim KS; Kumar NS; Gurusubramanian G
[Ad] Endereço:Department of Zoology, Mizoram Central University, Aizawl, Mizoram, India.
[Ti] Título:Biosterilant effects of Bacillus thuringiensis kurstaki HD-73 extract on male Wistar albino rats.
[So] Source:Theriogenology;88:73-83, 2017 Jan 15.
[Is] ISSN:1879-3231
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chemosterilants have long been used in sterilization programs for managing pet, stray, and wild animals but adverse effects such as trauma, incomplete responses to treatment, and complete abolition of gonadal sources of testosterone often occurs. This study describes the biosterilant effects of administering three doses of Bacillus thuringiensis kurstaki HD-73 (Bt 5, 50, and 250 mg/kg; 20 rats/dose) extract in adult male Wistar albino rats on testicular parameters, function, histology, and a number of biochemical markers of overall health, free radical production, and cell proliferation. Intratesticular administration of Bt extract to rats induces testicular oxidative stress and damages and consequently, perturb spermatogenesis, degeneration of testis, reduction in testes size, and depletion of testosterone and antioxidant enzyme concentrations in a dose-dependent manner because of free radical-mediated lipid peroxidation. No morbidity or mortality adverse effects were observed in both the saline control and Bt extract-treated rats. Significant variation was noted in clinical manifestations, weight and volume of testes, and hormonal and biochemical profiles between Bt doses in comparison with the saline control. Aspermia/azoospermia (100%) resulted in Bt-treated rats without any adverse effects. Histopathological analysis showed degeneration, necrosis, vacuolation, fewer germ cells, formation of multinucleated giant cells, and a lack of elongated spermatids in atrophic seminiferous tubules in Bt extract-treated groups in the presence of low concentrations of testosterone, antioxidant enzymes, and suppression of germ cell proliferation. Dose-dependent effects were evident in most parameters that were measured. The vast array of tests that were undertaken also provides some important indicators of the physiological effects associated with the treatments that were applied. Intratesticular injection of Bt extract impairs spermatogenesis and induces permanent sterility in rats.
[Mh] Termos MeSH primário: Bacillus thuringiensis/química
Esterilizantes Químicos/farmacologia
[Mh] Termos MeSH secundário: Animais
Asparaginase/efeitos dos fármacos
Glicemia/efeitos dos fármacos
Peso Corporal/efeitos dos fármacos
Relação Dose-Resposta a Droga
Ingestão de Alimentos/efeitos dos fármacos
Fígado/efeitos dos fármacos
Masculino
Tamanho do Órgão
Ratos
Ratos Wistar
Testículo/anatomia & histologia
Testículo/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Blood Glucose); 0 (Chemosterilants); EC 3.5.1.1 (Asparaginase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170512
[Lr] Data última revisão:
170512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161121
[St] Status:MEDLINE


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[PMID]:27779930
[Au] Autor:Baxter RH
[Ad] Endereço:Department of Chemistry Dept. of Molecular Biophysics & Biochemistry Yale University PO Box 208107, New Haven CT 06520-8107, USA. richard.baxter@yale.edu.
[Ti] Título:Chemosterilants for Control of Insects and Insect Vectors of Disease.
[So] Source:Chimia (Aarau);70(10):715-720, 2016 Oct.
[Is] ISSN:0009-4293
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Both historically and at present, vector control is the most generally effective means of controlling malaria transmission. Insecticides are the predominant method of vector control, but the sterile insect technique (SIT) is a complementary strategy with a successful track record in both agricultural and public health sectors. Strategies of genetic and radiation-induced sterilization of Anopheles have to date been limited by logistical and/or regulatory hurdles. A safe and effective mosquito chemosterilant would therefore be of major utility to future deployment of SIT for malaria control. Here we review the prior and current use of chemosterilants in SIT, and assess the potential for future research. Recent genomic and proteomic studies reveal opportunities for specific targeting of seminal fluid proteins, and the capacity to interfere with sperm motility and storage in the female.
[Mh] Termos MeSH primário: Esterilizantes Químicos/farmacologia
Insetos Vetores
Malária/prevenção & controle
Controle de Mosquitos/métodos
[Mh] Termos MeSH secundário: Animais
Aziridinas/farmacologia
Quitinases/antagonistas & inibidores
Hormônios Juvenis/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aziridines); 0 (Chemosterilants); 0 (Juvenile Hormones); 54P5FEX9FH (aziridine); EC 3.2.1.14 (Chitinases)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171001
[Lr] Data última revisão:
171001
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE


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[PMID]:27732882
[Au] Autor:Peng X; Gan J; Wang Q; Shi Z; Xia X
[Ad] Endereço:College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanixi, 712100, China.
[Ti] Título:3-Monochloro-1,2-propanediol (3-MCPD) induces apoptosis via mitochondrial oxidative phosphorylation system impairment and the caspase cascade pathway.
[So] Source:Toxicology;372:1-11, 2016 Nov 30.
[Is] ISSN:1879-3185
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:3-Monochloro-1,2-propanediol (3-MCPD) is the most toxic chloropropanols compounds in foodstuff which mainly generated during thermal processing. Kidney is one of the primary target organs for 3-MCPD. Using human embryonic kidney cell (HEK293FT) as an in vitro model, we found that 3-MCPD caused concentration-dependent increase in cytoxicity as assessed by dye uptake, lactatedehydrogenase (LDH) leakage and MTT assays. HEK293FT cell treated with 3-MCPD suffered the decrease of mitochondrial membrane potential and the impairment of mitochondrial oxidative phosphorylation system, especially the reduced amount of mRNA expression and protein synthesis of electron transport chain complex II, complex IV, and complex III. More importantly, energy release (ATP synthesis) was significantly inhibited by 3-MCPD resulting from the down regulation expressions of ATP synthase (ATP6 and ATP8), as well as the loss of transmembrane potential required for synthesis of ATP. The decreased ratio of mitochondrial apoptogenic factors Bax/Bcl-2 and the cytochrome-c release from mitochondria to cytosol followed by the activation of apoptotic initiators caspase 9 and apoptotic executioners (caspase 3, caspase 6 and caspase 7) leading to apoptosis. The activation of caspase 8 and caspase 2 implied that there were probably other factors to induce the caspase-dependent apoptosis.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Caspases/efeitos dos fármacos
Esterilizantes Químicos/toxicidade
Mitocôndrias/efeitos dos fármacos
Mitocôndrias/metabolismo
Fosforilação Oxidativa/efeitos dos fármacos
alfa-Cloridrina/toxicidade
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/biossíntese
Caspases/metabolismo
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Transporte de Elétrons/efeitos dos fármacos
Seres Humanos
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Potenciais da Membrana/efeitos dos fármacos
Espécies Reativas de Oxigênio/metabolismo
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chemosterilants); 0 (Reactive Oxygen Species); 8L70Q75FXE (Adenosine Triphosphate); 96-24-2 (alpha-Chlorohydrin); EC 3.4.22.- (Caspases)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170505
[Lr] Data última revisão:
170505
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161013
[St] Status:MEDLINE


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[PMID]:26657528
[Au] Autor:Garde E; Pérez GE; Vanderstichel R; Dalla Villa PF; Serpell JA
[Ad] Endereço:The Global Alliance for Animals and People, Pasaje Los Arrayanes 333, Valdivia 5110624, Chile; Veterinarians without Borders/Vétérinaires sans Frontiéres Canada. Electronic address: elena.garde@thegaap.org.
[Ti] Título:Effects of surgical and chemical sterilization on the behavior of free-roaming male dogs in Puerto Natales, Chile.
[So] Source:Prev Vet Med;123:106-120, 2016 Jan 01.
[Is] ISSN:1873-1716
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Population management of free-roaming domestic dogs (Canis lupus familiaris) is of interest due to the threat these animals pose to people, other animals and the environment. Current sterilization procedures for male dogs include surgical and chemical methods. However, little is known about how these procedures affect their behavior. The primary objective of this study was to investigate changes in selected behaviors following chemical and surgical sterilization in a male free-roaming dog (FRD) population in southern Chile. We also examined the association between serum testosterone levels and behaviors thought to be influenced by circulating androgens. A total of 174 dogs were randomly assigned to either a surgical or chemical sterilization group, or a control group. At the onset of the intervention period, 119 dogs remained and 102 dogs successfully completed the study. Each dog was monitored pre- and post-intervention using video recordings, GPS collars, and blood samples for the measurement of testosterone. Analysis of behavior revealed that surgically castrated dogs showed no reduction of sexual activity or aggression when compared to their pre-intervention behavior. Chemically sterilized dogs showed a statistically significant increase in dog-directed aggression, but no change in sexual activity. There was no change in home range size in any groups between the pre- and post-intervention measurement. We found no consistent association between levels of serum testosterone concentration and behavioral changes in any of the groups. This study presents the first detailed behavioral observations following surgical and chemical sterilization in male FRDs. The information generated is highly relevant to communities struggling with the control of FRDs. Complementary studies to further our understanding of the effects of male sterilization on the behavioral and reproductive dynamics of FRD populations are needed.
[Mh] Termos MeSH primário: Agressão
Esterilizantes Químicos/farmacologia
Cães/fisiologia
Gluconatos/farmacologia
Comportamento Sexual Animal
Esterilização Reprodutiva/veterinária
[Mh] Termos MeSH secundário: Agressão/efeitos dos fármacos
Animais
Arginina/administração & dosagem
Arginina/farmacologia
Esterilizantes Químicos/administração & dosagem
Chile
Cães/cirurgia
Análise Fatorial
Gluconatos/administração & dosagem
Masculino
Orquiectomia/veterinária
Comportamento Sexual Animal/efeitos dos fármacos
Esterilização Reprodutiva/métodos
Testosterona/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chemosterilants); 0 (Gluconates); 3XMK78S47O (Testosterone); 94ZLA3W45F (Arginine); R4R8J0Q44B (gluconic acid)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:170916
[Lr] Data última revisão:
170916
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151215
[St] Status:MEDLINE


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[PMID]:26025059
[Au] Autor:Camire RB; Beaulac HJ; Willis CL
[Ad] Endereço:Westbrook College of Health Professions, University of New England, Biddeford, ME 04005, USA. Electronic address: rcamire@une.edu.
[Ti] Título:Transitory loss of glia and the subsequent modulation in inflammatory cytokines/chemokines regulate paracellular claudin-5 expression in endothelial cells.
[So] Source:J Neuroimmunol;284:57-66, 2015 Jul 15.
[Is] ISSN:1872-8421
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Signaling mechanisms involved in regulating blood-brain barrier (BBB) integrity during central nervous system (CNS) inflammation remain unclear. We show that an imbalance between pro-/anti-inflammatory cytokines/chemokines alters claudin-5 expression. In vivo, gliotoxin-induced changes in glial populations and an imbalance between pro-/anti-inflammatory cytokine/chemokine expression occurred as BBB integrity was compromised. The balance was restored as BBB integrity was re-established. In vitro, TNF-α, IL-6, and MCP-1 induced paracellular claudin-5 expression loss. TNF-α- and IL-6- effects were mediated through the PI3K pathway and IL-10 attenuated TNF-α's effect. This study shows that pro-/anti-inflammatory modulators play a critical role in BBB integrity during CNS inflammation.
[Mh] Termos MeSH primário: Claudina-5/metabolismo
Citocinas/metabolismo
Células Endoteliais/metabolismo
Colículos Inferiores/citologia
Neuroglia/metabolismo
[Mh] Termos MeSH secundário: Animais
Antígeno CD11b/metabolismo
Linhagem Celular Transformada
Esterilizantes Químicos/farmacologia
Cromonas/farmacologia
Citocinas/farmacologia
Relação Dose-Resposta a Droga
Interações Medicamentosas
Células Endoteliais/efeitos dos fármacos
Inibidores Enzimáticos/farmacologia
Regulação da Expressão Gênica/efeitos dos fármacos
Proteína Glial Fibrilar Ácida/metabolismo
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Morfolinas/farmacologia
Neuroglia/efeitos dos fármacos
Ratos
Ratos Endogâmicos F344
Fatores de Tempo
alfa-Cloridrina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (CD11b Antigen); 0 (Chemosterilants); 0 (Chromones); 0 (Claudin-5); 0 (Cytokines); 0 (Enzyme Inhibitors); 0 (Glial Fibrillary Acidic Protein); 0 (Morpholines); 31M2U1DVID (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one); 96-24-2 (alpha-Chlorohydrin)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150531
[St] Status:MEDLINE


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[PMID]:25557187
[Au] Autor:Vanderstichel R; Forzán MJ; Pérez GE; Serpell JA; Garde E
[Ad] Endereço:Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Prince Edward Island, Canada. Electronic address: rvanderstich@upei.ca.
[Ti] Título:Changes in blood testosterone concentrations after surgical and chemical sterilization of male free-roaming dogs in southern Chile.
[So] Source:Theriogenology;83(6):1021-7, 2015 Apr 01.
[Is] ISSN:1879-3231
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:There is a growing interest in chemical sterilization as an alternative to surgical castration in large-scale sterilization campaigns to control canine populations. An important step toward understanding the short-term and long-term effects of chemical sterilants is to determine their impact on blood testosterone concentrations, particularly as these could influence dog behavior after treatment. A field trial was conducted with 118 free-roaming male dogs in the Chilean Patagonia, where 36 dogs were chemically sterilized using EsterilSol, 39 dogs were surgically castrated, and 43 dogs remained intact as controls. Blood testosterone levels were determined at four time periods: on enrollment 6 months before treatment (t-6m), at the time of treatment (t0, within one hour after surgical castration or chemical sterilization and during a concurrent 2-week period for the control group), four (t+4m), and six (t+6m) months after treatment. Intrinsic and temporal factors were evaluated; age was significantly associated with testosterone, where dogs 2- to 4-year-old had the highest testosterone concentrations (P = 0.036), whereas body weight and body condition scores were not associated with testosterone; testosterone concentration was not influenced by time of day, month, or season. After treatment (t+4m and t+6m), all of the surgically castrated dogs had testosterone concentrations below 1.0 ng/mL. On the basis of this cut point (<1 ng/mL), testosterone remained unchanged in 66% of the chemically sterilized dogs at both t+4m and t+6m; it remained low for 22% of dogs at both t+4m and t+6m; it was unchanged at t+4m but low at t+6m in 9% of dogs; and, it was low at t+4m but reverted back to unchanged at t+6m in one dog (3%). Incidentally, testosterone in chemically sterilized dogs increased dramatically within 1 hour of treatment (t0), more than doubling (131%) the concentration of control dogs at the time of treatment (t0), likely because of severe necrosis of interstitial cells. The use of EsterilSol as a method of sterilizing dogs had a variable effect on blood testosterone concentrations. Approximately, 30% of chemically sterilized dogs had a reduced testosterone concentration (actual maximum, 1 ng/mL) after 6 months, similar to that of surgically castrated dogs. Most chemically sterilized dogs, however, showed no long-term changes in blood testosterone concentrations.
[Mh] Termos MeSH primário: Esterilizantes Químicos/farmacologia
Cães
Gluconatos/farmacologia
Orquiectomia/veterinária
Esterilização Reprodutiva/veterinária
Testosterona/sangue
[Mh] Termos MeSH secundário: Animais
Gluconatos/administração & dosagem
Masculino
Orquiectomia/métodos
Esterilização Reprodutiva/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chemosterilants); 0 (Gluconates); 3XMK78S47O (Testosterone); R4R8J0Q44B (gluconic acid)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:150303
[Lr] Data última revisão:
150303
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150106
[St] Status:MEDLINE


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[PMID]:24888439
[Au] Autor:Le BV; Klöck C; Schatz A; Nguyen JB; Kakani EG; Catteruccia F; Khosla C; Baxter RH
[Ti] Título:Dihydroisoxazole inhibitors of Anopheles gambiae seminal transglutaminase AgTG3.
[So] Source:Malar J;13:210, 2014 Jun 02.
[Is] ISSN:1475-2875
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Current vector-based malaria control strategies are threatened by the rise of biochemical and behavioural resistance in mosquitoes. Researching mosquito traits of immunity and fertility is required to find potential targets for new vector control strategies. The seminal transglutaminase AgTG3 coagulates male Anopheles gambiae seminal fluids, forming a 'mating plug' that is required for male reproductive success. Inhibitors of AgTG3 can be useful both as chemical probes of A. gambiae reproductive biology and may further the development of new chemosterilants for mosquito population control. METHODS: A targeted library of 3-bromo-4,5-dihydroxoisoxazole inhibitors were synthesized and screened for inhibition of AgTG3 in a fluorescent, plate-based assay. Positive hits were tested for in vitro activity using cross-linking and mass spectrometry, and in vivo efficacy in laboratory mating assays. RESULTS: A targeted chemical library was screened for inhibition of AgTG3 in a fluorescent plate-based assay using its native substrate, plugin. Several inhibitors were identified with IC50 < 10 µM. Preliminary structure-activity relationships within the library support the stereo-specificity and preference for aromatic substituents in the chemical scaffold. Both inhibition of plugin cross-linking and covalent modification of the active site cysteine of AgTG3 were verified. Administration of an AgTG3 inhibitor to A. gambiae males by intrathoracic injection led to a 15% reduction in mating plug transfer in laboratory mating assays. CONCLUSIONS: A targeted screen has identified chemical inhibitors of A. gambiae transglutaminase 3 (AgTG3). The most potent inhibitors are known inhibitors of human transglutaminase 2, suggesting a common binding pose may exist within the active site of both enzymes. Future efforts to develop additional inhibitors will provide chemical tools to address important biological questions regarding the role of the A. gambiae mating plug. A second use for transglutaminase inhibitors exists for the study of haemolymph coagulation and immune responses to wound healing in insects.
[Mh] Termos MeSH primário: Anopheles/enzimologia
Esterilizantes Químicos/farmacologia
Proteínas de Insetos/antagonistas & inibidores
Isoxazóis/farmacologia
Controle de Mosquitos/métodos
Sêmen/enzimologia
Transglutaminases/antagonistas & inibidores
[Mh] Termos MeSH secundário: Animais
Domínio Catalítico
Esterilizantes Químicos/síntese química
Esterilizantes Químicos/química
Reagentes para Ligações Cruzadas/química
Reagentes para Ligações Cruzadas/farmacologia
Inibidores de Cisteína Proteinase/farmacologia
Avaliação Pré-Clínica de Medicamentos
Feminino
Seres Humanos
Concentração Inibidora 50
Isoxazóis/síntese química
Isoxazóis/química
Masculino
Modelos Moleculares
Estrutura Molecular
Conformação Proteica
Proteínas Recombinantes/efeitos dos fármacos
Bibliotecas de Moléculas Pequenas
Especificidade da Espécie
Relação Estrutura-Atividade
Especificidade por Substrato
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chemosterilants); 0 (Cross-Linking Reagents); 0 (Cysteine Proteinase Inhibitors); 0 (Insect Proteins); 0 (Isoxazoles); 0 (Recombinant Proteins); 0 (Small Molecule Libraries); EC 2.3.2.13 (TGM3 protein, human); EC 2.3.2.13 (Transglutaminases)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140604
[St] Status:MEDLINE
[do] DOI:10.1186/1475-2875-13-210


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[PMID]:24513037
[Au] Autor:Gato R; Companioni A; Bruzón RY; Menéndez Z; González A; Rodríguez M
[Ad] Endereço:Instituto de Medicina Tropical Pedro Kourí, Cuba. Electronic address: rene@ipk.sld.cu.
[Ti] Título:Release of thiotepa sterilized males into caged populations of Aedes aegypti: life table analysis.
[So] Source:Acta Trop;132 Suppl:S164-9, 2014 Apr.
[Is] ISSN:1873-6254
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Successful SIT trials against mosquitoes in the 1960-70s were achieved by sterilizing male mosquitoes using chemosterilants. Their use was discontinued after concerns were raised about the effect of residues on non-target organisms, although scant evidence has been published. Irradiation is an expensive process; chemosterilization could be an affordable option for implementing SIT programs in developing countries. We compare life table parameters of three Aedes aegypti populations comprising different ratios of thiotepa-treated and non-treated males in order to identify the impact on reproductive potential of the presence of sterile males. No difference was observed in the survival of the treated and untreated males. The release of thiotepa sterilized males into caged Ae. aegypti populations had no effect on death or survival probability of the individuals in the cages but the fecundity of females was significantly reduced, as evaluated by hatch rate and stable age structure parameters. The significant decreases in net reproduction rate, finite rate of natural increase and intrinsic rate of natural increase in populations including sterile males are sufficient to indicate that such populations would not be able to proliferate in natural conditions. This suggests that release of Ae. aegypti thiotepa-treated males could be effective in reducing the reproductive capability of the target population and consequently contribute to vector control.
[Mh] Termos MeSH primário: Aedes/efeitos dos fármacos
Aedes/crescimento & desenvolvimento
Esterilizantes Químicos/metabolismo
Esterilização Reprodutiva/métodos
Tiotepa/metabolismo
[Mh] Termos MeSH secundário: Animais
Feminino
Fertilidade
Tábuas de Vida
Masculino
Controle Biológico de Vetores/métodos
Reprodução
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chemosterilants); 905Z5W3GKH (Thiotepa)
[Em] Mês de entrada:1411
[Cu] Atualização por classe:140326
[Lr] Data última revisão:
140326
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140212
[St] Status:MEDLINE


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[PMID]:24498756
[Au] Autor:Juan-Blasco M; Urbaneja A; San Andrés V; Castañera P; Sabater-Muñoz B
[Ad] Endereço:Instituto Valenciano de Investigaciones Agrarias, Centro de Protección Vegetal y Biotecnología, Unidad Asociada de Entomología, Moncada, Spain.
[Ti] Título:Improving the sterile sperm identification method for its implementation in the Area-wide Sterile Insect Technique Program against Ceratitis capitata (Diptera: Tephritidae) in Spain.
[So] Source:J Econ Entomol;106(6):2541-7, 2013 Dec.
[Is] ISSN:0022-0493
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The success of sterile males in area-wide sterile insect technique (aw-SIT) programs against Ceratitis capitata (Wiedemann) is currently measured by using indirect methods as the wild:sterile male ratio captured in monitoring traps. In the past decade, molecular techniques have been used to improve these methods. The development of a polymerase chain reaction-restriction fragment-length polymorphism-based method to identify the transfer of sterile sperm to wild females, the target of SIT, was considered a significant step in this direction. This method relies on identification of sperm by detecting the presence of Y chromosomes in spermathecae DNA extract complemented by the identification of the genetic origin of this sperm: Vienna-8 males or wild haplotype. However, the application of this protocol to aw-SIT programs is limited by handling time and personnel cost. The objective of this work was to obtain a high-throughput protocol to facilitate the routine measurement in a pest population of sterile sperm presence in wild females. The polymerase chain reaction-restriction fragment-length polymorphism markers previously developed were validated in Mediterranean fruit fly samples collected from various locations worldwide. A laboratory protocol previously published was modified to allow for the analysis of more samples at the same time. Preservation methods and preservation times commonly used for Mediterranean fruit fly female samples were assessed for their influence on the correct molecular detection of sterile sperm. This high-throughput methodology, as well as the results of sample management presented here, provide a robust, efficient, fast, and economical sterile sperm identification method ready to be used in all Mediterranean fruit fly SIT programs.
[Mh] Termos MeSH primário: Ceratitis capitata/fisiologia
Controle Biológico de Vetores/métodos
Reação em Cadeia da Polimerase/métodos
Espermatozoides/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Ceratitis capitata/efeitos dos fármacos
Ceratitis capitata/genética
Esterilizantes Químicos/farmacologia
Feminino
Masculino
Polimorfismo de Fragmento de Restrição
Espermatozoides/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Chemosterilants)
[Em] Mês de entrada:1402
[Cu] Atualização por classe:140206
[Lr] Data última revisão:
140206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140207
[St] Status:MEDLINE


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[PMID]:23998740
[Au] Autor:Massei G; Miller LA
[Ad] Endereço:National Wildlife Management Centre, Animal Health and Veterinary Laboratories Agency, Sand Hutton, York YO26 5LE, UK. Electronic address: giovanna.massei@ahvla.gsi.gov.uk.
[Ti] Título:Nonsurgical fertility control for managing free-roaming dog populations: a review of products and criteria for field applications.
[So] Source:Theriogenology;80(8):829-38, 2013 Nov.
[Is] ISSN:1879-3231
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:About 75% of dogs worldwide are free to roam and reproduce, thus creating locally overabundant populations. Problems caused by roaming dogs include diseases transmitted to livestock and humans, predation on livestock, attacks on humans, road traffic accidents, and nuisance behavior. Nonsurgical fertility control is increasingly advocated as more cost-effective than surgical sterilization to manage dog populations and their impact. The aims of this review were to 1) analyze trends in numbers of scientific publications on nonsurgical fertility control for dogs; 2) illustrate the spectrum of fertility inhibitors available for dogs; 3) examine how differences between confined and free-roaming dogs might affect the choice of fertility inhibitors to be used in dog population management; and 4) provide a framework of criteria to guide decisions regarding the use of nonsurgical fertility control for dog population management. The results showed that the 117 articles published between 1982 and 2011 focussed on long-term hormonal contraceptives, such as gonadotropin-releasing hormone agonists, immunocontraceptives, and male chemical sterilants. The number of articles published biennially increased from one to five papers produced in the early 1980s to 10 to 20 in the past decade. Differences between confined dogs and free-roaming dogs include reproduction and survival as well as social expectations regarding the duration of infertility, the costs of sterilization, and the responsibilities for meeting these costs. These differences are likely to dictate which fertility inhibitors will be used for confined or free-roaming dogs. The criteria regarding the use of fertility control for dog population management, presented as a decision tree, covered social acceptance, animal welfare, effectiveness, legal compliance, feasibility, and sustainability. The review concluded that the main challenges for the future are evaluating the feasibility, effectiveness, sustainability, and effects of mass nonsurgical sterilization campaigns on dog population size and impact as well as integrating nonsurgical fertility control with disease vaccination and public education programs.
[Mh] Termos MeSH primário: Anticoncepção/veterinária
Cães/fisiologia
[Mh] Termos MeSH secundário: Animais
Esterilizantes Químicos/uso terapêutico
Anticoncepção/métodos
Anticoncepção Imunológica/veterinária
Feminino
Masculino
Densidade Demográfica
Esterilização Reprodutiva/métodos
Esterilização Reprodutiva/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Chemosterilants)
[Em] Mês de entrada:1406
[Cu] Atualização por classe:130927
[Lr] Data última revisão:
130927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130904
[St] Status:MEDLINE



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