Base de dados : MEDLINE
Pesquisa : D27.720.355 [Categoria DeCS]
Referências encontradas : 5145 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 515 ir para página                         

  1 / 5145 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29024724
[Au] Autor:Urban C; Buck A; Siveke JT; Lordick F; Luber B; Walch A; Aichler M
[Ad] Endereço:Research Unit Analytical Pathology, Helmholtz Zentrum München, Neuherberg, Germany. Electronic address: christian.urban@helmholtz-muenchen.de.
[Ti] Título:PAXgene fixation enables comprehensive metabolomic and proteomic analyses of tissue specimens by MALDI MSI.
[So] Source:Biochim Biophys Acta;1862(1):51-60, 2018 01.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:An alcohol-based non-crosslinking tissue fixative, PAXgene Tissue System, has been proposed as alternative fixation method to formalin, providing superior and morphological preservation. To date, metabolites have not been assessed in PAXgene-fixed tissues. The study focuses on a comparison between PAXgene and standard formalin fixation for metabolomic analysis by MALDI mass spectrometry imaging. Therefore, fifty-six samples from seven mice organs were fixed with PAXgene (PFPE) or formalin (FFPE), embedded in paraffin, and processed to a tissue microarray. PAXgene was able to spatially preserve metabolites in organs achieving an overlap of common metabolites ranging from 34 to 78% with FFPE. Highly similar signal intensities and visualization of molecules demonstrated negligible differences for metabolite imaging on PFPE compared to FFPE tissues. In addition, we performed proteomic analysis of intact proteins and peptides derived from enzymatic digestion. An overlap of 33 to 58% was found between FFPE and PFPE tissue samples in peptide analysis with a higher number of PFPE-specific peaks. Analysis of intact proteins achieved an overlap in the range of 0 to 28% owing to the poor detectability of cross-linked proteins in formalin-fixed tissues. Furthermore, metabolite and peptide profiles obtained from PFPE tissues were able to correctly classify organs independent of the fixation method, whereas a distinction of organs by protein profiles was only achieved by PAXgene fixation. Finally, we applied MALDI MSI to human biopsies by sequentially analyzing metabolites and peptides within the same tissue section. Concerning prospective studies, PAXgene can be used as an alternative fixative for multi-omic tissue analysis.
[Mh] Termos MeSH primário: Fixadores/química
Metabolômica/métodos
Proteômica/métodos
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
Fixação de Tecidos/métodos
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Camundongos
Peptídeos/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fixatives); 0 (Peptides)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171013
[St] Status:MEDLINE


  2 / 5145 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28969954
[Au] Autor:Marcano V; Cardenas-Garcia S; Gogal RM; Afonso CL
[Ad] Endereço:Department of Veterinary Pathology, College of Veterinary Medicine, The University of Georgia, 501, D. W. Brooks Dr., Athens, GA 30601, USA; Southeast Poultry Research Laboratory, 934 College Station Rd., Athens, GA, 30605, USA. Electronic address: vcm6@uga.edu.
[Ti] Título:Intracellular fixation buffer inactivates Newcastle disease virus in chicken allantoic fluid, macrophages and splenocytes.
[So] Source:J Virol Methods;251:1-6, 2018 Jan.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Inactivation of Newcastle disease virus (NDV) has been routinely achieved with heat, ß-propiolactone, binary ethylenimine, ultraviolet light and formalin. However, these strategies have not been tested for cell surface ligand or receptor phenotype in viral-infected chicken immune cells. To study the capacity of fixation buffers to preserve surface markers while inactivating NDV, a primary splenocyte culture was infected with NDV and incubated with a commercial intracellular fixation buffer (ICB), formulated with 4% formaldehyde. Splenocytes were fixed with a 1:2 dilution of ICB in phosphate buffered saline (PBS) for 45min at 23°C or 4°C and inactivation of NDV was tested in addition to recognition of antigens by antibodies in fixed and non-fixed splenocytes via flow cytometric analysis. The binding and percentage of splenic CD4+ and CD8+ cells were not affected. In addition, NDV titers as high as 10 and 10 EID in allantoic fluid (AF) and macrophages, respectively, were successfully inactivated after 45min at 23°C and 4°C, confirming the ICB's effectiveness in inactivating high concentrations of NDV. In conclusion, high concentrations of NDV in AF, chicken splenocytes, and macrophages can be inactivated using ICB. Additionally, this method did not compromise cell phenotyping of enriched chicken splenocytes.
[Mh] Termos MeSH primário: Desinfetantes/farmacologia
Fixadores/farmacologia
Leucócitos/virologia
Viabilidade Microbiana/efeitos dos fármacos
Vírus da Doença de Newcastle/efeitos dos fármacos
Inativação de Vírus
[Mh] Termos MeSH secundário: Alantoide/virologia
Animais
Células Cultivadas
Galinhas
Vírus da Doença de Newcastle/fisiologia
Baço/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Disinfectants); 0 (Fixatives)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171004
[St] Status:MEDLINE


  3 / 5145 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28823320
[Au] Autor:Yamamoto Y; Iino K; Shintani Y; Kato H; Kimura K; Watanabe G; Takemura H
[Ad] Endereço:Department of Thoracic, Cardiovascular and General Surgery, Kanazawa University, Kanazawa, Japan. Electronic address: yamamoto054@gmail.com.
[Ti] Título:Comparison of Aortic Annulus Dimension After Aortic Valve Neocuspidization With Valve Replacement and Normal Valve.
[So] Source:Semin Thorac Cardiovasc Surg;29(2):143-149, 2017 Summer.
[Is] ISSN:1532-9488
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Aortic valve replacement (AVR) remains the standard surgical intervention for aortic valve disease and is preferred by many surgeons, despite its associated clinical issues. The clinical efficacy of aortic valve neocuspidization (AVNeo) with glutaraldehyde-treated autologous pericardium, the Ozaki procedure, has recently been reported. Although it is presumed to preserve the normal aortic annulus motion, changes to the aortic annulus during the cardiac cycle after AVNeo remain unclear. From March to December 2014, aortic annular dimensions were measured for 23 patients; the sample included 8 patients who had undergone AVNeo, 10 patients with normal aortic valves, and 5 patients who had undergone AVR. Measurements were recorded using electrocardiography-gated multidetector computed tomography. Data were analyzed using automated aortic root analysis software. Postoperative peak pressure gradients for the AVNeo and AVR groups were compared. No statistically significant differences in annulus variation were observed between patients who had undergone AVNeo and those with normal aortic valves. Annular area was larger during systole than during diastole in both groups. Postoperative peak pressure gradients were significantly lower in the AVNeo group than in the AVR group. The results of the present study demonstrated that aortic annular dimensions after AVNeo are similar to the dimensions of normal aortic valves. This was evidenced using electrocardiography-gated multidetector computed tomography, previously reported as the most reliable method, to evaluate annulus motion during the cardiac cycle. Lower postoperative peak pressure gradients might underlie the observed changes. These advantages will help in rectifying AVR defects.
[Mh] Termos MeSH primário: Valva Aórtica/cirurgia
Bioprótese
Doenças das Valvas Cardíacas/cirurgia
Implante de Prótese de Valva Cardíaca/instrumentação
Próteses Valvulares Cardíacas
Pericárdio/transplante
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Valva Aórtica/diagnóstico por imagem
Valva Aórtica/fisiopatologia
Autoenxertos
Técnicas de Imagem de Sincronização Cardíaca
Estudos de Casos e Controles
Eletrocardiografia
Feminino
Fixadores
Glutaral
Doenças das Valvas Cardíacas/diagnóstico por imagem
Doenças das Valvas Cardíacas/fisiopatologia
Implante de Prótese de Valva Cardíaca/efeitos adversos
Implante de Prótese de Valva Cardíaca/métodos
Hemodinâmica
Seres Humanos
Masculino
Meia-Idade
Tomografia Computadorizada Multidetectores
Valor Preditivo dos Testes
Desenho de Prótese
Interpretação de Imagem Radiográfica Assistida por Computador
Recuperação de Função Fisiológica
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fixatives); T3C89M417N (Glutaral)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170822
[St] Status:MEDLINE


  4 / 5145 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28796828
[Au] Autor:Bussolati G; Annaratone L; Berrino E; Miglio U; Panero M; Cupo M; Gugliotta P; Venesio T; Sapino A; Marchiò C
[Ad] Endereço:Department of Medical Sciences, University of Turin, Turin, Italy.
[Ti] Título:Acid-free glyoxal as a substitute of formalin for structural and molecular preservation in tissue samples.
[So] Source:PLoS One;12(8):e0182965, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tissue fixation in phosphate buffered formalin (PBF) remains the standard procedure in histopathology, since it results in an optimal structural, antigenic and molecular preservation that justifies the pivotal role presently played by diagnoses on PBF-fixed tissues in precision medicine. However, toxicity of formaldehyde causes an environmental concern and may demand substitution of this reagent. Having observed that the reported drawbacks of commercially available glyoxal substitutes of PBF (Prefer, Glyo-fix, Histo-Fix, Histo-CHOICE, and Safe-Fix II) are likely related to their acidity, we have devised a neutral fixative, obtained by removing acids from the dialdehyde glyoxal with an ion-exchange resin. The resulting glyoxal acid-free (GAF) fixative has been tested in a cohort of 30 specimens including colon (N = 25) and stomach (N = 5) cancers. Our results show that GAF fixation produces a tissue and cellular preservation similar to that produced by PBF. Comparable immuno-histochemical and molecular (DNA and RNA) analytical data were obtained. We observed a significant enrichment of longer DNA fragment size in GAF-fixed compared to PBF-fixed samples. Adoption of GAF as a non-toxic histological fixative of choice would require a process of validation, but the present data suggest that it represents a reliable candidate.
[Mh] Termos MeSH primário: Fixadores/química
Formaldeído/química
Glioxal/química
Fixação de Tecidos/métodos
[Mh] Termos MeSH secundário: DNA/análise
Seres Humanos
Imuno-Histoquímica/métodos
Hibridização in Situ Fluorescente/métodos
RNA/análise
Análise de Sequência de DNA/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fixatives); 1HG84L3525 (Formaldehyde); 50NP6JJ975 (Glyoxal); 63231-63-0 (RNA); 9007-49-2 (DNA)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170811
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182965


  5 / 5145 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28673821
[Au] Autor:Li Y; Almassalha LM; Chandler JE; Zhou X; Stypula-Cyrus YE; Hujsak KA; Roth EW; Bleher R; Subramanian H; Szleifer I; Dravid VP; Backman V
[Ad] Endereço:Applied Physics Program, Northwestern University, Evanston, IL, USA. Electronic address: yueli2014@u.northwestern.edu.
[Ti] Título:The effects of chemical fixation on the cellular nanostructure.
[So] Source:Exp Cell Res;358(2):253-259, 2017 Sep 15.
[Is] ISSN:1090-2422
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chemical fixation is nearly indispensable in the biological sciences, especially in circumstances where cryo-fixation is not applicable. While universally employed for the preservation of cell organization, chemical fixatives often introduce artifacts that can confound identification of true structures. Since biological research is increasingly probing ever-finer details of the cellular architecture, it is critical to understand the nanoscale transformation of the cellular organization due to fixation both systematically and quantitatively. In this work, we employed Partial Wave Spectroscopic (PWS) Microscopy, a nanoscale sensitive and label-free live cell spectroscopic-imaging technique, to analyze the effects of the fixation process through three commonly used fixation protocols for cells in vitro. In each method investigated, we detected dramatic difference in both nuclear and cytoplasmic nanoarchitecture between live and fixed states. But significantly, despite the alterations in cellular nanoscale organizations after chemical fixation, the population differences in chromatin structure (e.g. induced by a specific chemotherapeutic agent) remains. In conclusion, we demonstrated that the nanoscale cellular arrangement observed in fixed cells was fundamentally divorced from that in live cells, thus the quantitative analysis is only meaningful on the population level. This finding highlights the importance of live cell imaging techniques with nanoscale sensitivity or cryo-fixation in the interrogation of cellular structure, to complement more traditional chemical fixation methods.
[Mh] Termos MeSH primário: Fixadores/metabolismo
Nanoestruturas
[Mh] Termos MeSH secundário: Animais
Artefatos
Criopreservação/instrumentação
Seres Humanos
Imagem por Ressonância Magnética/métodos
Microscopia/métodos
Fixação de Tecidos/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fixatives)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170705
[St] Status:MEDLINE


  6 / 5145 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28655757
[Au] Autor:Chew TG; Huang J; Palani S; Sommese R; Kamnev A; Hatano T; Gu Y; Oliferenko S; Sivaramakrishnan S; Balasubramanian MK
[Ad] Endereço:Warwick Medical School, University of Warwick, Coventry, UK t.g.chew@warwick.ac.uk.
[Ti] Título:Actin turnover maintains actin filament homeostasis during cytokinetic ring contraction.
[So] Source:J Cell Biol;216(9):2657-2667, 2017 Sep 04.
[Is] ISSN:1540-8140
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cytokinesis in many eukaryotes involves a tension-generating actomyosin-based contractile ring. Many components of actomyosin rings turn over during contraction, although the significance of this turnover has remained enigmatic. Here, using , we investigate the role of turnover of actin and myosin II in its contraction. Actomyosin ring components self-organize into ∼1-µm-spaced clusters instead of undergoing full-ring contraction in the absence of continuous actin polymerization. This effect is reversed when actin filaments are stabilized. We tested the idea that the function of turnover is to ensure actin filament homeostasis in a synthetic system, in which we abolished turnover by fixing rings in cell ghosts with formaldehyde. We found that these rings contracted fully upon exogenous addition of a vertebrate myosin. We conclude that actin turnover is required to maintain actin filament homeostasis during ring contraction and that the requirement for turnover can be bypassed if homeostasis is achieved artificially.
[Mh] Termos MeSH primário: Citoesqueleto de Actina/metabolismo
Actinas/metabolismo
Actomiosina/metabolismo
Citocinese
Schizosaccharomyces/metabolismo
[Mh] Termos MeSH secundário: Fixadores/química
Formaldeído/química
Homeostase
Microscopia Confocal
Microscopia de Vídeo
Schizosaccharomyces/genética
Fatores de Tempo
Imagem com Lapso de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; VIDEO-AUDIO MEDIA
[Nm] Nome de substância:
0 (Actins); 0 (Fixatives); 1HG84L3525 (Formaldehyde); 9013-26-7 (Actomyosin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170629
[St] Status:MEDLINE
[do] DOI:10.1083/jcb.201701104


  7 / 5145 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28644855
[Au] Autor:Vojtechova Z; Zavadil J; Klozar J; Grega M; Tachezy R
[Ad] Endereço:Department of Genetics and Microbiology, Faculty of Science, Charles University, Prague, Czech Republic.
[Ti] Título:Comparison of the miRNA expression profiles in fresh frozen and formalin-fixed paraffin-embedded tonsillar tumors.
[So] Source:PLoS One;12(6):e0179645, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:MicroRNAs are considered as promising prognostic and diagnostic biomarkers of human cancer since their profiles differ between tumor types. Most of the tumor profiling studies were performed on rarely available fresh frozen (FF) samples. Alternatively, archived formalin-fixed paraffin-embedded (FFPE) tissue samples are also well applicable to larger-scale retrospective miRNA profiling studies. The aim of this study was to perform systematic comparison of the miRNA expression profiles between FF and macrodissected FFPE tonsillar tumors using the TaqMan Low Density Array system, with the data processed by different software programs and two types of normalization methods. We observed a marked correlation between the miRNA expression profiles of paired FF and FFPE samples; however, only 27-38% of the differentially deregulated miRNAs overlapped between the two source systems. The comparison of the results with regard to the distinct modes of data normalization revealed an overlap in 58-67% of differentially expressed miRNAs, with no influence of the choice of software platform. Our study highlights the fact that for an accurate comparison of the miRNA expression profiles from published studies, it is important to use the same type of clinical material and to test and select the best-performing normalization method for data analysis.
[Mh] Termos MeSH primário: Criopreservação
MicroRNAs/metabolismo
Inclusão em Parafina
Neoplasias Tonsilares/metabolismo
[Mh] Termos MeSH secundário: Fixadores
Formaldeído
Perfilação da Expressão Gênica
Seres Humanos
Análise em Microsséries
Tonsila Palatina/metabolismo
Análise de Componente Principal
Processamento de Sinais Assistido por Computador
Software
Fixação de Tecidos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fixatives); 0 (MicroRNAs); 1HG84L3525 (Formaldehyde)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170624
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179645


  8 / 5145 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28624625
[Au] Autor:Low YS; Blöcker C; McPherson JR; Tang SA; Cheng YY; Wong JYS; Chua C; Lim TKH; Tang CL; Chew MH; Tan P; Tan IB; Rozen SG; Cheah PY
[Ad] Endereço:Department of Colorectal Surgery, Singapore General Hospital, 169856, Singapore.
[Ti] Título:A formalin-fixed paraffin-embedded (FFPE)-based prognostic signature to predict metastasis in clinically low risk stage I/II microsatellite stable colorectal cancer.
[So] Source:Cancer Lett;403:13-20, 2017 Sep 10.
[Is] ISSN:1872-7980
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Approximately 20% early-stage (I/II) colorectal cancer (CRC) patients develop metastases despite curative surgery. We aim to develop a formalin-fixed and paraffin-embedded (FFPE)-based predictor of metastases in early-stage, clinically-defined low risk, microsatellite-stable (MSS) CRC patients. We considered genome-wide mRNA and miRNA expression and mutation status of 20 genes assayed in 150 fresh-frozen tumours with known metastasis status. We selected 193 genes for further analysis using NanoString nCounter arrays on corresponding FFPE tumours. Neither mutation status nor miRNA expression improved the estimated prediction. The final predictor, ColoMet19, based on the top 19 genes' mRNA levels trained by Random Forest machine-learning strategy, had an estimated positive-predictive-value (PPV) of 0.66. We tested ColoMet19 on an independent test-set of 131 tumours and obtained a population-adjusted PPV of 0.67 indicating that early-stage CRC patients who tested positive have a 67% risk of developing metastases, substantially higher than the metastasis risk of 40% for node-positive (Stage III) patients who are generally treated with chemotherapy. Predicted-positive patients also had poorer metastasis-free survival (hazard ratios [HR] = 1.92, design-set; HR = 2.05, test-set). Thus, early-stage CRC patients who test positive may be considered for adjuvant therapy after surgery.
[Mh] Termos MeSH primário: Biomarcadores Tumorais/genética
Neoplasias Colorretais/genética
Fixadores/química
Formaldeído/química
Perfilação da Expressão Gênica/métodos
Repetições de Microssatélites
Inclusão em Parafina
Fixação de Tecidos/métodos
Transcriptoma
[Mh] Termos MeSH secundário: Idoso
Área Sob a Curva
Neoplasias Colorretais/mortalidade
Neoplasias Colorretais/patologia
Neoplasias Colorretais/terapia
Análise Mutacional de DNA
Intervalo Livre de Doença
Feminino
Predisposição Genética para Doença
Estudo de Associação Genômica Ampla
Seres Humanos
Estimativa de Kaplan-Meier
Masculino
MicroRNAs/genética
Mutação
Metástase Neoplásica
Estadiamento de Neoplasias
Fenótipo
Valor Preditivo dos Testes
Modelos de Riscos Proporcionais
RNA Mensageiro/genética
Curva ROC
Reprodutibilidade dos Testes
Medição de Risco
Fatores de Risco
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (Fixatives); 0 (MicroRNAs); 0 (RNA, Messenger); 1HG84L3525 (Formaldehyde)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170619
[St] Status:MEDLINE


  9 / 5145 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28596153
[Au] Autor:Grillo F; Bruzzone M; Pigozzi S; Prosapio S; Migliora P; Fiocca R; Mastracci L
[Ad] Endereço:Pathology Unit, Department of Surgical Science and Integrated Diagnostics (DISC), University of Genoa, Genoa, Italy.
[Ti] Título:Immunohistochemistry on old archival paraffin blocks: is there an expiry date?
[So] Source:J Clin Pathol;70(11):988-993, 2017 Nov.
[Is] ISSN:1472-4146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Few studies have focused on antigen preservation in formalin-fixed and paraffin-embedded (FFPE) tissue in old archival material and additional studies are required, especially considering that these samples are an irreplaceable resource for scientific and clinical research. The purpose of this study is to verify antigen preservation in FFPE tissue samples stored for several decades. From the pathology archives, FFPE blocks were selected dating back to the 1960s, 1970s, 1980s, 1990s, 2000s and 2010. A panel of 12 antibodies was applied and immunoreactivities were compared. While cytoplasmic antigens showed no reduction in immunostaining intensity over time, membrane and nuclear antigens presented reduced staining intensity in older blocks. In particular, the nuclear antigen, Ki67 and CD31 showed the most pronounced antigen decay in the oldest archival blocks. In order to test possible antigen recovery, deep sectioning and lengthening of heat pretreatment were applied. Both strategies partially recover antigenicity, but their simultaneous application shows the best results.
[Mh] Termos MeSH primário: Fixadores
Formaldeído
Imuno-Histoquímica
Antígeno Ki-67/análise
Inclusão em Parafina
Molécula-1 de Adesão Celular Endotelial de Plaquetas/análise
Manejo de Espécimes/métodos
Fixação de Tecidos/métodos
[Mh] Termos MeSH secundário: Seres Humanos
Valor Preditivo dos Testes
Estabilidade Proteica
Proteólise
Reprodutibilidade dos Testes
Fatores de Tempo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fixatives); 0 (Ki-67 Antigen); 0 (Platelet Endothelial Cell Adhesion Molecule-1); 1HG84L3525 (Formaldehyde)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170610
[St] Status:MEDLINE
[do] DOI:10.1136/jclinpath-2017-204387


  10 / 5145 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28571924
[Au] Autor:Naik SM; Anil AC
[Ad] Endereço:Academy of Scientific and Innovative Research (AcSIR), CSIR-National Institute of Oceanography (NIO), Dona Paula 403 004, Goa, India.
[Ti] Título:Long-term preservation of Tetraselmis indica (Chlorodendrophyceae, Chlorophyta) for flow cytometric analysis: Influence of fixative and storage temperature.
[So] Source:J Microbiol Methods;139:123-129, 2017 Aug.
[Is] ISSN:1872-8359
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Immediate enumeration of phytoplankton is seldom possible. Therefore, fixation and subsequent storage are required for delayed analysis. This study investigated the influence of glutaraldehyde (GA) concentrations (0.25%, 0.5%, and 1%) and storage temperatures (-80°C , -80°C, -20°C, and 5°C) on Tetraselmis indica for flow cytometric analysis. Cell recovery, granularity, and membrane permeability were independent of GA concentration whereas cell size and chlorophyll autofluorescence were concentration dependent. After an initial cell loss (16-19%), no cell loss was observed when samples were stored at 5°C. Cell recovery was not influenced by storage temperature until 4months but later samples preserved at -80°C , -80°C, and -20°C resulted in ~41% cell loss. Although maximum cell recovery with minimal effect on cell integrity was obtained at 5°C, autofluorescence was retained better at -80°C and -80°C. This suggests that in addition to fixative, the choice of storage temperature is equally important. Thus for long-term preservation, especially to retain autofluorescence, the use of lower concentration (0.25%) of GA when stored at a lower temperature (-80°C and -80°C) while a higher concentration (1%) of GA when stored at a higher temperature (5°C) is recommended.
[Mh] Termos MeSH primário: Clorófitas
Temperatura Baixa
Fixadores/farmacologia
Citometria de Fluxo/métodos
Glutaral/farmacologia
Preservação Biológica/métodos
[Mh] Termos MeSH secundário: Sobrevivência Celular
Clorófitas/fisiologia
Fluorescência
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fixatives); T3C89M417N (Glutaral)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170603
[St] Status:MEDLINE



página 1 de 515 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde