Base de dados : MEDLINE
Pesquisa : D27.720.470 [Categoria DeCS]
Referências encontradas : 93 [refinar]
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[PMID]:25082738
[Au] Autor:Eifler N; Medaglia G; Anderka O; Laurin L; Hermans P
[Ad] Endereço:Dept. BPRD Protein Processing, Technical Research and Development, Novartis Pharma AG, Werk Klybeck, Basel, CH-4002, Switzerland.
[Ti] Título:Development of a novel affinity chromatography resin for platform purification of lambda fabs.
[So] Source:Biotechnol Prog;30(6):1311-8, 2014 Nov-Dec.
[Is] ISSN:1520-6033
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Antigen-binding fragments (Fabs) are novel formats in the growing pipeline of biotherapeutics. Sharing similar features to monoclonal antibodies (mAbs) with regard to expression, Fabs are considered as unchallenging for upstream development. Yet for downstream processing, the mature mAb downstream purification platform is not directly applicable. New approaches need to be found to achieve a lean purification process that maintains quality, productivity, and timelines while being generically applicable independent of the expression system. In a successful collaboration, BAC BV, GE Healthcare, and Novartis Pharma AG have developed a new affinity chromatography medium (resin) suitable to support cGMP manufacturing of lambda Fabs. We show that using this novel chromatography medium for the capture step, a purification platform for lambda Fabs can be established.
[Mh] Termos MeSH primário: Cromatografia de Afinidade/instrumentação
Cromatografia de Afinidade/métodos
Fragmentos Fab das Imunoglobulinas
Reagentes de Laboratório
[Mh] Termos MeSH secundário: Animais
Células CHO
Cricetinae
Cricetulus
Escherichia coli
Seres Humanos
Fragmentos Fab das Imunoglobulinas/química
Fragmentos Fab das Imunoglobulinas/isolamento & purificação
Fragmentos Fab das Imunoglobulinas/metabolismo
Reagentes de Laboratório/química
Reagentes de Laboratório/metabolismo
Ligação Proteica
Proteínas Recombinantes/química
Proteínas Recombinantes/isolamento & purificação
Proteínas Recombinantes/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunoglobulin Fab Fragments); 0 (Laboratory Chemicals); 0 (Recombinant Proteins)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:141208
[Lr] Data última revisão:
141208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140802
[St] Status:MEDLINE
[do] DOI:10.1002/btpr.1958


  2 / 93 MEDLINE  
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[PMID]:25080096
[Au] Autor:Siu SC; Chia C; Mok Y; Pattnaik P
[Ad] Endereço:Manufacturing Science and Technology, Roche Singapore Technical Operations Pte Ltd., Singapore.
[Ti] Título:Packing of large-scale chromatography columns with irregularly shaped glass based resins using a stop-flow method.
[So] Source:Biotechnol Prog;30(6):1319-25, 2014 Nov-Dec.
[Is] ISSN:1520-6033
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Rigid chromatography resins, such as controlled pore glass based adsorbents, offer the advantage of high permeability and a linear pressure-flow relationship irrespective of column diameter which improves process time and maximizes productivity. However, the rigidity and irregularly shaped nature of these resins often present challenges in achieving consistent and uniform packed beds as formation of bridges between resin particles can hinder bed consolidation. The standard flow-pack method when applied to irregularly shaped particles does not yield well-consolidated packed beds, resulting in formation of a head space and increased band broadening during operation. Vibration packing methods requiring the use of pneumatically driven vibrators are recommended to achieve full packed bed consolidation but limitations in manufacturing facilities and equipment may prevent the implementation of such devices. The stop-flow packing method was developed as an improvement over the flow-pack method to overcome these limitations and to improve bed consolidation without the use of vibrating devices. Transition analysis of large-scale columns packed using the stop-flow method over multiple cycles has shown a two- to three-fold reduction of change in bed integrity values as compared to a flow-packed bed demonstrating an improvement in packed bed stability in terms of the height equivalent to a theoretical plate (HETP) and peak asymmetry (As ).
[Mh] Termos MeSH primário: Cromatografia Líquida/instrumentação
Vidro/química
Modelos Teóricos
[Mh] Termos MeSH secundário: Reagentes de Laboratório
Reologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Laboratory Chemicals)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:150115
[Lr] Data última revisão:
150115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140801
[St] Status:MEDLINE
[do] DOI:10.1002/btpr.1962


  3 / 93 MEDLINE  
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[PMID]:24814006
[Au] Autor:Pezzini J; Cabanne C; Dupuy JW; Gantier R; Santarelli X
[Ad] Endereço:Univ. Bordeaux, EA 4135, F-33000 Bordeaux, France; IPB, EA 4135, F-33000 Bordeaux, France; Pall Life Sciences, 48, avenue des Genottes, 95800 Cergy Saint-Christophe, France; Pall Life Sciences, 50 Bearfoot Road, Northborough, MA 01532, USA.
[Ti] Título:A study on the nature of interactions of mixed-mode ligands HEA and PPA HyperCel using phenylglyoxal modified lysozyme.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;960:209-13, 2014 Jun 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Mixed mode chromatography, or multimodal chromatography, involves the exploitation of combinations of several interactions in a controlled manner, to facilitate the rapid capture of proteins. Mixed-mode ligands like HEA and PPA HyperCel™ facilitate different kinds of interactions (hydrophobic, ionic, etc.) under different conditions. In order to better characterize the nature of this multi-modal interaction, we sought to study a protein, lysozyme, which is normally not retained by these mixed mode resins under normal binding conditions. Lysozyme was modified specifically at Arginine residues by the action of phenylglyoxal, and was extensively studied in this work to better characterize the mixed-mode interactions of HEA HyperCel™ and PPA HyperCel™ chromatographic supports. We show here that the adsorption behaviour of lysozyme on HEA and PPA HyperCel™ mixed mode sorbents varies depending on the degree of charge modification at the surface of the protein. Experiments using conventional cation exchange and hydrophobic interaction chromatography confirm that both charge and hydrophobicity modification occurs at the surface of the protein after lysozyme reaction with phenylglyoxal. The results emanating from this work using HEA and PPA HyperCel sorbents strongly suggest that mixed mode chromatography can efficiently separate closely related proteins of only minor surface charge and/or hydrophobicity differences.
[Mh] Termos MeSH primário: Cromatografia Líquida/instrumentação
Cromatografia Líquida/métodos
Muramidase/química
Fenilglioxal/química
[Mh] Termos MeSH secundário: Adsorção
Interações Hidrofóbicas e Hidrofílicas
Reagentes de Laboratório
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Laboratory Chemicals); EC 3.2.1.17 (Muramidase); N45G3015PA (Phenylglyoxal)
[Em] Mês de entrada:1501
[Cu] Atualização por classe:140523
[Lr] Data última revisão:
140523
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140513
[St] Status:MEDLINE


  4 / 93 MEDLINE  
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[PMID]:24814003
[Au] Autor:Liu J; Li Q; Liu R; Yin Y; Chen X; Bi K
[Ad] Endereço:School of Chinese Material Medica, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China.
[Ti] Título:Enrichment and purification of six Aconitum alkaloids from Aconiti kusnezoffii radix by macroporous resins and quantification by HPLC-MS.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;960:174-81, 2014 Jun 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Aconitine, mesaconitine, hypaconitine, benzoylaconine, benzoylmesaconine and benzoylhypaconine are six main Aconitum alkaloids from traditional Chinese medicine, Aconiti kusnezoffii radix, which possess highly bioactive as well as highly toxic character for medicinal use. In the present study, for the purpose of better utilizing the toxic herbal material, the performance characteristics of NKA-II, D101, X-5, AB-8, S-8, HPD722 and HPD750 macroporous resins for the enrichment and purification of these six Aconitum alkaloids were critically evaluated. Results showed that NKA-II offered the best adsorption and desorption capacities for six Aconitum alkaloids among the seven macroporous resins tested, which were affected significantly by the pH value. Subsequently, dynamic adsorption and desorption experiments had been carried out with the column packed by NKA-II resin to optimize the separation process of six Aconitum alkaloids. After one run treatment with NKA-II resin, the content of total six Aconitum alkaloids were increased from 5.87% to 60.3%, the recovery was 75.8%. Meanwhile, a validated HPLC-MS method had been developed to qualitative and quantitative these six Aconitum alkaloids. This method would provide scientific references to the large-scale production of six Aconitum alkaloids from Aconiti kusnezoffii radix or other plants and might also expand the secure application of these highly toxic components for pharmacy.
[Mh] Termos MeSH primário: Aconitum/química
Alcaloides/isolamento & purificação
Cromatografia Líquida de Alta Pressão/métodos
Espectrometria de Massas/métodos
Extratos Vegetais/química
[Mh] Termos MeSH secundário: Adsorção
Alcaloides/análise
Alcaloides/química
Concentração de Íons de Hidrogênio
Reagentes de Laboratório/química
Porosidade
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Laboratory Chemicals); 0 (Plant Extracts)
[Em] Mês de entrada:1501
[Cu] Atualização por classe:140523
[Lr] Data última revisão:
140523
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140513
[St] Status:MEDLINE


  5 / 93 MEDLINE  
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[PMID]:24801195
[Au] Autor:Rodrigues T; Schneider P; Schneider G
[Ad] Endereço:Eidgenössische Technische Hochschule (ETH) Zürich, Departement für Chemie und Angewandte Biowissenschaften, Vladimir-Prelog-Weg 4, 8093 Zürich (Switzerland).
[Ti] Título:Accessing new chemical entities through microfluidic systems.
[So] Source:Angew Chem Int Ed Engl;53(23):5750-8, 2014 Jun 02.
[Is] ISSN:1521-3773
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Flow systems have been successfully utilized for a wide variety of applications in chemical research and development, including the miniaturization of (bio)analytical methods and synthetic (bio)organic chemistry. Currently, we are witnessing the growing use of microfluidic technologies for the discovery of new chemical entities. As a consequence, chemical biology and molecular medicine research are being reshaped by this technique. In this Minireview we portray the state-of-the-art, including the most recent advances in the application of microchip reactors as well as the micro- and mesoscale coil reactor-assisted synthesis of bioactive small molecules, and forecast the potential future use of this promising technology.
[Mh] Termos MeSH primário: Técnicas Analíticas Microfluídicas/métodos
Microfluídica/métodos
[Mh] Termos MeSH secundário: Reagentes de Laboratório
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Laboratory Chemicals)
[Em] Mês de entrada:1504
[Cu] Atualização por classe:140603
[Lr] Data última revisão:
140603
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140508
[St] Status:MEDLINE
[do] DOI:10.1002/anie.201400988


  6 / 93 MEDLINE  
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[PMID]:24685908
[Au] Autor:Pecinka A; Liu CH
[Ad] Endereço:Department of Plant Breeding and Genetics, Max Planck Institute for Plant Breeding Research, Cologne, Germany.
[Ti] Título:Drugs for plant chromosome and chromatin research.
[So] Source:Cytogenet Genome Res;143(1-3):51-9, 2014.
[Is] ISSN:1424-859X
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Eukaryotic genomes are organized into chromosomes. Genetic information regularly becomes damaged and requires repair in order to ensure genome stability. Furthermore, expression of individual genetic elements on the chromosome(s) is controlled by several factors, including chromatin. Understanding the functions of chromatin may provide efficient tools for regulating gene expression. There has been great progress in understanding genome control using genetic mutations, but the use of mutants is sometimes not possible or may require additional interference with DNA or chromatin structure using specific treatments in order to obtain phenotypes. Therefore, chemical genetics has become an integral part of plant genome research. Here, we summarize information on the most commonly used drugs for chromatin and DNA damage repair studies, with the aim of simplifying the choice of drug and the estimation of possible side effects for current and future research.
[Mh] Termos MeSH primário: Cromatina/efeitos dos fármacos
Cromossomos de Plantas/efeitos dos fármacos
Reparo do DNA/efeitos dos fármacos
Reagentes de Laboratório/farmacologia
[Mh] Termos MeSH secundário: Animais
Cromatina/genética
Cromossomos de Plantas/genética
Reparo do DNA/genética
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Chromatin); 0 (Laboratory Chemicals)
[Em] Mês de entrada:1410
[Cu] Atualização por classe:140820
[Lr] Data última revisão:
140820
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140402
[St] Status:MEDLINE
[do] DOI:10.1159/000360774


  7 / 93 MEDLINE  
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[PMID]:24662143
[Au] Autor:Chen T; Liu YL; Chen C; Zou DL; You JM; Sun J; Li YL
[Ad] Endereço:Key Laboratory of Tibetan medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, P.R. China.
[Ti] Título:Application of high-speed counter-current chromatography combined with macroporous resin for rapid enrichment and separation of three anthraquinone glycosides and one stilbene glycoside from Rheum tanguticum.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;957:90-5, 2014 Apr 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this paper, an efficient method was successfully established by the combination of macroporous resin (MR) and high-speed counter-current chromatography (HSCCC) for rapid enrichment and separation of aloe-emodin 8-O-ß-D-glucoside, emodin 1-O-ß-D-glucoside, emodin 8-O-ß-D-glucoside and piceatannol 4'-O-ß-D-(6″-O-gallate)-glucoside. Six kinds of macroporous resins were investigated in the first step and X-5 macroporous resin was selected for the enrichment of the target compounds. The recoveries of the target compounds reached 89.0, 85.9, 82.3 and 84.9% respectively after 40% ethanol elution. In the second step, the target compounds were separated by HSCCC with a two-phase solvent system composed of chloroform/ethyl acetate/methanol/water (8:1:6:5, v/v). The established method will be helpful for further characterization and utilization of Rheum tanguticum. The results demonstrate that MR coupled with HSCCC is a powerful technique for separation of bioactive compounds from natural products.
[Mh] Termos MeSH primário: Antraquinonas/isolamento & purificação
Distribuição Contracorrente/métodos
Glicosídeos/isolamento & purificação
Rheum/química
Estilbenos/isolamento & purificação
[Mh] Termos MeSH secundário: Antraquinonas/análise
Antraquinonas/química
Glicosídeos/análise
Glicosídeos/química
Reagentes de Laboratório
Extratos Vegetais/química
Raízes de Plantas/química
Porosidade
Estilbenos/análise
Estilbenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anthraquinones); 0 (Glycosides); 0 (Laboratory Chemicals); 0 (Plant Extracts); 0 (Stilbenes)
[Em] Mês de entrada:1411
[Cu] Atualização por classe:140401
[Lr] Data última revisão:
140401
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140326
[St] Status:MEDLINE


  8 / 93 MEDLINE  
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[PMID]:24660226
[Au] Autor:Nybo K
[Ti] Título:Troubleshooting forum. Molecular biology techniques Q&A.
[So] Source:Biotechniques;56(2):59, 2014.
[Is] ISSN:1940-9818
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Separação Celular/métodos
Centrifugação/métodos
[Mh] Termos MeSH secundário: Ficoll/química
Reagentes de Laboratório/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Laboratory Chemicals); 25702-74-3 (Ficoll)
[Em] Mês de entrada:1409
[Cu] Atualização por classe:140321
[Lr] Data última revisão:
140321
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140325
[St] Status:MEDLINE


  9 / 93 MEDLINE  
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[PMID]:24643870
[Au] Autor:Chuffart F; Yvert G
[Ad] Endereço:Laboratoire de Biologie Moléculaire de la Cellule, Ecole Normale Supérieure de Lyon, CNRS, Université de Lyon, France.
[Ti] Título:MyLabStocks: a web-application to manage molecular biology materials.
[So] Source:Yeast;31(5):179-84, 2014 May.
[Is] ISSN:1097-0061
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Laboratory stocks are the hardware of research. They must be stored and managed with mimimum loss of material and information. Plasmids, oligonucleotides and strains are regularly exchanged between collaborators within and between laboratories. Managing and sharing information about every item is crucial for retrieval of reagents, for planning experiments and for reproducing past experimental results. We have developed a web-based application to manage stocks commonly used in a molecular biology laboratory. Its functionalities include user-defined privileges, visualization of plasmid maps directly from their sequence and the capacity to search items from fields of annotation or directly from a query sequence using BLAST. It is designed to handle records of plasmids, oligonucleotides, yeast strains, antibodies, pipettes and notebooks. Based on PHP/MySQL, it can easily be extended to handle other types of stocks and it can be installed on any server architecture. MyLabStocks is freely available from: https://forge.cbp.ens-lyon.fr/redmine/projects/mylabstocks under an open source licence.
[Mh] Termos MeSH primário: Bases de Dados Genéticas/provisão & distribuição
Biologia Molecular/instrumentação
Ácidos Nucleicos/provisão & distribuição
Software
[Mh] Termos MeSH secundário: Internet
Laboratórios/provisão & distribuição
Reagentes de Laboratório/provisão & distribuição
Plasmídeos/provisão & distribuição
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Laboratory Chemicals); 0 (Nucleic Acids)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140320
[St] Status:MEDLINE
[do] DOI:10.1002/yea.3008


  10 / 93 MEDLINE  
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[PMID]:24641474
[Au] Autor:Perkel JM
[Ti] Título:The antibody challenge.
[So] Source:Biotechniques;56(3):111-4, 2014.
[Is] ISSN:1940-9818
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Anticorpos/imunologia
Técnicas Imunológicas/normas
[Mh] Termos MeSH secundário: Animais
Especificidade de Anticorpos
Biomarcadores
Seres Humanos
Técnicas Imunológicas/instrumentação
Reagentes de Laboratório/normas
Editoração/normas
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:NEWS
[Nm] Nome de substância:
0 (Antibodies); 0 (Biomarkers); 0 (Laboratory Chemicals)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140320
[St] Status:MEDLINE
[do] DOI:10.2144/000114143



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