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  1 / 20641 MEDLINE  
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[PMID]:28470607
[Au] Autor:Britton J; Smith JN; Raston CL; Weiss GA
[Ad] Endereço:Department of Chemistry, University of California, Irvine, 1102 Natural Sciences 2, Irvine, CA, 92697-2025, USA.
[Ti] Título:Protein Folding Using a Vortex Fluidic Device.
[So] Source:Methods Mol Biol;1586:211-220, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Essentially all biochemistry and most molecular biology experiments require recombinant proteins. However, large, hydrophobic proteins typically aggregate into insoluble and misfolded species, and are directed into inclusion bodies. Current techniques to fold proteins recovered from inclusion bodies rely on denaturation followed by dialysis or rapid dilution. Such approaches can be time consuming, wasteful, and inefficient. Here, we describe rapid protein folding using a vortex fluidic device (VFD). This process uses mechanical energy introduced into thin films to rapidly and efficiently fold proteins. With the VFD in continuous flow mode, large volumes of protein solution can be processed per day with 100-fold reductions in both folding times and buffer volumes.
[Mh] Termos MeSH primário: Hidrodinâmica
Muramidase/química
Dobramento de Proteína
[Mh] Termos MeSH secundário: Animais
Tampões (Química)
Galinhas
Desenho de Equipamento
Escherichia coli/genética
Expressão Gênica
Muramidase/genética
Física/instrumentação
Desnaturação Proteica
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Buffers); 0 (Recombinant Proteins); EC 3.2.1.- (hen egg lysozyme); EC 3.2.1.17 (Muramidase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-6887-9_13


  2 / 20641 MEDLINE  
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[PMID]:29304068
[Au] Autor:Drake AC; Lee Y; Burgess EM; Karlsson JOM; Eroglu A; Higgins AZ
[Ad] Endereço:School of Chemical, Biological and Environmental Engineering, Oregon State University, Corvallis, Oregon, United States of America.
[Ti] Título:Effect of water content on the glass transition temperature of mixtures of sugars, polymers, and penetrating cryoprotectants in physiological buffer.
[So] Source:PLoS One;13(1):e0190713, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Long-term storage of viable mammalian cells is important for applications ranging from in vitro fertilization to cell therapy. Cryopreservation is currently the most common approach, but storage in liquid nitrogen is relatively costly and the requirement for low temperatures during shipping is inconvenient. Desiccation is an alternative strategy with the potential to enable viable cell preservation at more convenient storage temperatures without the need for liquid nitrogen. To achieve stability during storage in the dried state it is necessary to remove enough water that the remaining matrix forms a non-crystalline glassy solid. Thus, the glass transition temperature is a key parameter for design of cell desiccation procedures. In this study, we have investigated the effects of moisture content on the glass transition temperature (Tg) of mixtures of sugars (trehalose or raffinose), polymers (polyvinylpyrrolidone or Ficoll), penetrating cryoprotectants (ethylene glycol, propylene glycol, or dimethyl sulfoxide), and phosphate buffered saline (PBS) solutes. Aqueous solutions were dried to different moisture contents by equilibration with saturated salt solutions, or by baking at 95°C. The glass transition temperatures of the dehydrated samples were then measured by differential scanning calorimetry. As expected, Tg increased with decreasing moisture content. For example, in a desiccation medium containing 0.1 M trehalose in PBS, Tg ranged from about 360 K for a completely dry sample to about 220 K at a water mass fraction of 0.4. Addition of polymers to the solutions increased Tg, while addition of penetrating cryoprotectants decreased Tg. Our results provide insight into the relationship between relative humidity, moisture content and glass transition temperature for cell desiccation solutions containing sugars, polymers and penetrating cryoprotectants.
[Mh] Termos MeSH primário: Crioprotetores/química
Polímeros/química
Açúcares/química
Temperatura de Transição
Água/química
[Mh] Termos MeSH secundário: Tampões (Química)
Varredura Diferencial de Calorimetria
Criopreservação/métodos
Dessecação/métodos
Dimetil Sulfóxido/química
Etilenoglicol/química
Ficoll/química
Vidro/química
Modelos Teóricos
Povidona/química
Propilenoglicol/química
Rafinose/química
Soluções/química
Trealose/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Buffers); 0 (Cryoprotective Agents); 0 (Polymers); 0 (Solutions); 0 (Sugars); 059QF0KO0R (Water); 25702-74-3 (Ficoll); 6DC9Q167V3 (Propylene Glycol); B8WCK70T7I (Trehalose); FC72KVT52F (Ethylene Glycol); FZ989GH94E (Povidone); N5O3QU595M (Raffinose); YOW8V9698H (Dimethyl Sulfoxide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190713


  3 / 20641 MEDLINE  
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[PMID]:28453926
[Au] Autor:Eberhart MS; Wee KR; Marquard S; Skinner K; Wang D; Nayak A; Meyer TJ
[Ad] Endereço:Department of Chemistry, University of North Carolina at Chapel Hill, CB 3290, Chapel Hill, North Carolina, 27599, USA.
[Ti] Título:Fluoropolymer-Stabilized Chromophore-Catalyst Assemblies in Aqueous Buffer Solutions for Water-Oxidation Catalysis.
[So] Source:ChemSusChem;10(11):2380-2384, 2017 06 09.
[Is] ISSN:1864-564X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Here, the application of the fluorinated polymer [Dupont AF, a copolymer of 4,5-difluoro-2,2-bis(trifluoromethyl)-1,3-dioxole and tetrafluoroethylene] is described in stabilizing phosphonate-derivatized molecular assemblies on oxide electrodes. In the procedure, the polymer was dip-coated onto the surfaces of oxide electrodes with pre-bound, phosphonate-derivatized chromophores and assemblies, including assemblies for water oxidation. The results of the experiments showed a high degree of stabilization by the added polymer and a demonstration of its use in stabilizing surface-bound assemblies for water-oxidation catalysis.
[Mh] Termos MeSH primário: Polímeros/química
Água/química
[Mh] Termos MeSH secundário: Tampões (Química)
Catálise
Eletroquímica/métodos
Eletrodos
Oxirredução
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Buffers); 0 (Polymers); 059QF0KO0R (Water)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180117
[Lr] Data última revisão:
180117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1002/cssc.201700630


  4 / 20641 MEDLINE  
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[PMID]:29227597
[Au] Autor:Yatsenko TA; Rybachuk VM; Yusova OI; Kharchenko SM; Grinenko TV
[Ti] Título:Effect of fibrin degradation products on fibrinolytic process.
[So] Source:Ukr Biochem J;88(2):16-24, 2016 Mar-Apr.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:Fibrin clot lysis by plasminogen/plasmin system results in fibrin degradation products formation with subsequent release into bloodstream. The fragments contain specific binding sites for fibrinolytic system components and can interact with them. In this study, we investigated the way in which fibrin fragments effect fibrinolytic process. We have shown that high molecular weight products of fibrin degradation and fibrin fragments of DDE-complex and DD, but not end product Е3, stimulate plasmin formation. Additionally, components of DDE-complex mixture of fragments Е1 and Е2 have potentiation ability. The intermediate fibrin fragments hmFDPs and DDE attenuate clot lysis by plasmin and hmFDPs protect plasmin from α2-antiplasmin inhibition but under further fragmentation to endpoint fibrin fragments loose this ability. The plasma inhibitors reduce fibrinolytic system activity generated by the degradation products. Thus, fibrin fragments formed during the clot lysis can bind and move out fibrinolytic system components from clot volume and in this way result in clot resistance to hydrolysis.
[Mh] Termos MeSH primário: Produtos de Degradação da Fibrina e do Fibrinogênio/isolamento & purificação
Fibrina/química
Fibrinolisina/química
Plasminogênio/química
Ativador de Plasminogênio Tecidual/química
alfa 2-Antiplasmina/química
[Mh] Termos MeSH secundário: Tampões (Química)
Cromatografia em Gel
Cromatografia por Troca Iônica
Ativação Enzimática
Produtos de Degradação da Fibrina e do Fibrinogênio/química
Fibrinólise/fisiologia
Seres Humanos
Cinética
Ligação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Buffers); 0 (Fibrin Fibrinogen Degradation Products); 0 (alpha-2-Antiplasmin); 9001-31-4 (Fibrin); 9001-91-6 (Plasminogen); EC 3.4.21.68 (PLAT protein, human); EC 3.4.21.68 (Tissue Plasminogen Activator); EC 3.4.21.7 (Fibrinolysin)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.02.016


  5 / 20641 MEDLINE  
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[PMID]:28466818
[Au] Autor:Algul S; Ozcelik O; Yilmaz B
[Ad] Endereço:Faculty of Medicine, Department of Physiology, YuzuncuYil University, Van, Turkey.
[Ti] Título:Evaluation of relationship between aerobic fitness level and range of isocapnic buffering periods during incremental exercise test.
[So] Source:Cell Mol Biol (Noisy-le-grand);63(3):78-82, 2017 Mar 31.
[Is] ISSN:1165-158X
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:The purpose of this study is to examine the relationship between the amount of O2 uptake (VO2) in the range of isocapnic buffering (ICB) periods and aerobic fitness levels of subjects with different exercise tolerance levels. A total of 50 young male subjects (20.8±0.4 years) performed an incremental exercise test using a cycle ergometer to determinetheir anaerobic threshold (AT), respiratory compensation point (RCP) and maximal exercise capacity (Wmax). The ICB period is defined as the region between AT and RCP. Pulmonary gas exchange parameters were measured breath-by-breath using a respiratory gas analyser. The subjects' fitness levels, as indicated by peak O2 uptake to body weight ratios (VO2peak/BW), ranged from 28 ml/min/kg to 58 ml/min/kg, and Wmax capacity to body weight ratio (Wmax/BW) ranged from 1.94 W/min/kg to 3.96 W/min/kg. The VO2 in the range of ICB periodsranged from 101 ml to 793 ml (with an average of 295±157 ml). There was a positive linear correlation between VO2peak/BW, Wmax/BW and range of ICB: R=0.76542 (p<0.0001), and R=0.92135 (p<0.0001), respectively. The results of this study suggest that the range of ICB periods may be related to aerobic fitness. Importantly, aerobic fitness levels should be evaluated and considered important data, in addition toAT, VO2peak/BW and Wmax/BW.
[Mh] Termos MeSH primário: Dióxido de Carbono/metabolismo
Teste de Esforço
Exercício
Aptidão Física
[Mh] Termos MeSH secundário: Limiar Anaeróbio
Peso Corporal
Tampões (Química)
Seres Humanos
Masculino
Consumo de Oxigênio
Troca Gasosa Pulmonar
Adulto Jovem
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Buffers); 142M471B3J (Carbon Dioxide)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180115
[Lr] Data última revisão:
180115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.14715/cmb/2017.63.3.15


  6 / 20641 MEDLINE  
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[PMID]:29253861
[Au] Autor:Eich G; Bartosova M; Tischer C; Wlodkowski TT; Schaefer B; Pichl S; Kraewer N; Ranchin B; Vondrak K; Liebau MC; Hackert T; Schmitt CP
[Ad] Endereço:Center for Pediatric and Adolescent Medicine, University Hospital Heidelberg, Heidelberg, Germany.
[Ti] Título:Bicarbonate buffered peritoneal dialysis fluid upregulates angiopoietin-1 and promotes vessel maturation.
[So] Source:PLoS One;12(12):e0189903, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Ultrafiltration decline is a progressive issue for patients on chronic peritoneal dialysis (PD) and can be caused by peritoneal angiogenesis induced by PD fluids. A recent pediatric trial suggests better preservation of ultrafiltration with bicarbonate versus lactate buffered fluid; underlying molecular mechanisms are unknown. METHODS: Angiogenic cytokine profile, tube formation capacity and Receptor Tyrosine Kinase translocation were assessed in primary human umbilical vein endothelial cells following incubation with bicarbonate (BPDF) and lactate buffered (LPDF), pH neutral PD fluid with low glucose degradation product content and lactate buffered, acidic PD fluid with high glucose degradation product content (CPDF). Peritoneal biopsies from age-, PD-vintage- and dialytic glucose exposure matched, peritonitis-free children on chronic PD underwent automated histomorphometry and immunohistochemistry. RESULTS: In endothelial cells angiopoietin-1 mRNA and protein abundance increased 200% upon incubation with BPDF, but decreased by 70% with LPDF as compared to medium control; angiopoietin-2 remained unchanged. Angiopoietin-1/Angiopoietin-2 protein ratio was 15 and 3-fold increased with BPDF compared to LPDF and medium. Time-lapse microscopy with automated network analysis demonstrated less endothelial cell tube formation with BPDF compared to LPDF and CPDF incubation. Receptor Tyrosine Kinase translocated to the cell membrane in BPDF but not in LPDF or CPDF incubated endothelial cells. In children dialyzed with BPDF peritoneal vessels were larger and angiopoietin-1 abundance in CD31 positive endothelium higher compared to children treated with LPDF. CONCLUSION: Bicarbonate buffered PD fluid promotes vessel maturation via upregulation of angiopoietin-1 in vitro and in children on dialysis. Our findings suggest a molecular mechanism for the observed superior preservation of ultrafiltration capacity with bicarbonate buffered PD fluid with low glucose degradation product content.
[Mh] Termos MeSH primário: Angiopoietina-1/metabolismo
Bicarbonatos/química
Tampões (Química)
Diálise Peritoneal
[Mh] Termos MeSH secundário: Adolescente
Angiopoietina-2/metabolismo
Biópsia
Criança
Doença Crônica
Citocinas/metabolismo
Células Endoteliais/metabolismo
Glucose/química
Células Endoteliais da Veia Umbilical Humana
Seres Humanos
Concentração de Íons de Hidrogênio
Nefropatias/terapia
Lactatos/química
Peritônio/patologia
Molécula-1 de Adesão Celular Endotelial de Plaquetas/metabolismo
Receptores Proteína Tirosina Quinases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ANGPT1 protein, human); 0 (ANGPT2 protein, human); 0 (Angiopoietin-1); 0 (Angiopoietin-2); 0 (Bicarbonates); 0 (Buffers); 0 (Cytokines); 0 (Lactates); 0 (Platelet Endothelial Cell Adhesion Molecule-1); EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180112
[Lr] Data última revisão:
180112
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189903


  7 / 20641 MEDLINE  
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[PMID]:28468478
[Au] Autor:Finsen V
[Ad] Endereço:Det medisinske fakultet Norges teknisk-naturvitenskapelige universitet og Ortopedisk avdeling St. Olavs hospital.
[Ti] Título:Reduced pain when injecting lidocaine.
[Ti] Título:Mindre smerte ved lokalbedøvelse..
[So] Source:Tidsskr Nor Laegeforen;137(9):629-630, 2017 05.
[Is] ISSN:0807-7096
[Cp] País de publicação:Norway
[La] Idioma:eng; nor
[Ab] Resumo:Pain on injection of lidocaine is often considered a necessary evil, but it can be reduced by simple means.
[Mh] Termos MeSH primário: Anestésicos Locais
Lidocaína
Dor
[Mh] Termos MeSH secundário: Anestesia Local/instrumentação
Anestesia Local/métodos
Anestésicos Locais/administração & dosagem
Anestésicos Locais/efeitos adversos
Bicarbonatos/administração & dosagem
Tampões (Química)
Temperatura Alta
Seres Humanos
Injeções Subcutâneas/instrumentação
Injeções Subcutâneas/métodos
Lidocaína/administração & dosagem
Lidocaína/efeitos adversos
Agulhas
Dor/induzido quimicamente
Dor/prevenção & controle
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anesthetics, Local); 0 (Bicarbonates); 0 (Buffers); 98PI200987 (Lidocaine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171208
[Lr] Data última revisão:
171208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.4045/tidsskr.16.0515


  8 / 20641 MEDLINE  
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[PMID]:28846405
[Au] Autor:Shi RY; Hong ZN; Li JY; Jiang J; Baquy MA; Xu RK; Qian W
[Ad] Endereço:State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences , P.O. Box 821, Nanjing 210008, P.R. China.
[Ti] Título:Mechanisms for Increasing the pH Buffering Capacity of an Acidic Ultisol by Crop Residue-Derived Biochars.
[So] Source:J Agric Food Chem;65(37):8111-8119, 2017 Sep 20.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The effects and underlying mechanisms of crop residue-derived biochars on the pH buffering capacity (pHbuff) of an acidic Ultisol, with low pHbuff, were investigated through indoor incubation and simulated acidification experiments. The incorporation of biochars significantly increased soil pHbuff with the magnitude of the increase dependent on acid buffering capacity of the biochar incorporated to the soil. Cation release, resulting from the protonation of carboxyl groups on biochar surfaces and the dissolution of carbonates, was the predominant mechanism responsible for the increase in soil pHbuff at pH 4.0-7.0 and accounted for >67% of the increased pHbuff. The reaction of protons with soluble silica (Si) in biochars derived from rice straw and corn stover also accounted for ∼20% of the pHbuff increase due to H SiO precipitation. In conclusion, the incorporation of crop residue-derived biochars into acidic soils increased soil pHbuff with peanut stover biochar being the most effective biochar tested.
[Mh] Termos MeSH primário: Arachis/química
Carvão Vegetal/química
Oryza/química
Solo/química
Zea mays/química
[Mh] Termos MeSH secundário: Ácidos/química
Tampões (Química)
Concentração de Íons de Hidrogênio
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acids); 0 (Buffers); 0 (Soil); 0 (biochar); 16291-96-6 (Charcoal)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170829
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b02266


  9 / 20641 MEDLINE  
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[PMID]:28838788
[Au] Autor:Ren Y; Chen J; Shi Y; Li X; Yang N; Wang X
[Ad] Endereço:Jiangsu Key Laboratory of Anaerobic Biotechnology, Jiangsu Cooperative Innovation Center of Technology and Material of Water Treatment, School of Environmental and Civil Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address: ypren@jiangnan.edu.cn.
[Ti] Título:Anolyte recycling enhanced bioelectricity generation of the buffer-free single-chamber air-cathode microbial fuel cell.
[So] Source:Bioresour Technol;244(Pt 1):1183-1187, 2017 Nov.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Anolyte acidification is an inevitable restriction for the bioelectricity generation of buffer-free microbial fuel cells (MFCs). In this work, acidification of the buffer-free KCl anolyte has been thoroughly eliminated through anolyte recycling. The accumulated HCO concentration in the recycled KCl anolyte was above 50mM, which played as natural buffer and elevated the anolyte pH to above 8. The maximum power density (P ) increased from 322.9mWm to 527.2mWm , which is comparable with the phosphate buffered MFC. Besides Geobacter genus, the gradually increased anolyte pH and conductivity induced the growing of electrochemically active Geoalkalibacter genus, in the anode biofilm. Anolyte recycling is a feasible strategy to strengthen the self-buffering capacity of buffer-free MFCs, thoroughly eliminate the anolyte acidification and prominently enhance the electric power.
[Mh] Termos MeSH primário: Fontes de Energia Bioelétrica
Geobacter
[Mh] Termos MeSH secundário: Tampões (Química)
Eletricidade
Eletrodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Buffers)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170826
[St] Status:MEDLINE


  10 / 20641 MEDLINE  
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[PMID]:28796475
[Au] Autor:Kim C; Searson PC
[Ad] Endereço:Department of Materials Science and Engineering, Johns Hopkins University , 3400 North Charles Street, Baltimore, Maryland 21218, United States.
[Ti] Título:Detection of Plasmodium Lactate Dehydrogenase Antigen in Buffer Using Aptamer-Modified Magnetic Microparticles for Capture, Oligonucleotide-Modified Quantum Dots for Detection, and Oligonucleotide-Modified Gold Nanoparticles for Signal Amplification.
[So] Source:Bioconjug Chem;28(9):2230-2234, 2017 Sep 20.
[Is] ISSN:1520-4812
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To overcome the limitations associated with antibody-based sensors, we describe a proof-of-concept of an aptamer-based sandwich assay for detection of lactate dehydrogenase, an antigen associated with malaria. We show a detection limit of Plasmodium falciparum lactate dehydrogenase and Plasmodium vivax lactate dehydrogenase of 0.5 fmole in buffer, comparable to an antibody-based assay, using a magnetic particle-aptamer construct for capture and a quantum dot-aptamer construct for detection. We then demonstrate a detection limit of 10 amole (50-fold amplification) using oligonucleotide-functionalized gold nanoparticles to allow the conjugation of multiple quantum dots for each target antigen.
[Mh] Termos MeSH primário: Antígenos de Protozoários/análise
Aptâmeros de Nucleotídeos/química
Técnicas Biossensoriais/métodos
Ouro/química
L-Lactato Desidrogenase/análise
Plasmodium vivax/enzimologia
Pontos Quânticos/química
[Mh] Termos MeSH secundário: Tampões (Química)
Seres Humanos
Limite de Detecção
Imãs/química
Malária Vivax/parasitologia
Oligonucleotídeos/química
Plasmodium vivax/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Protozoan); 0 (Aptamers, Nucleotide); 0 (Buffers); 0 (Oligonucleotides); 7440-57-5 (Gold); EC 1.1.1.27 (L-Lactate Dehydrogenase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170811
[St] Status:MEDLINE
[do] DOI:10.1021/acs.bioconjchem.7b00328



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde