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[PMID]:29431326
[Au] Autor:Khripach LV; Zheleznyak EV; Knyazeva TD; Koganova ZI; Salikhova DI; Grishin DA
[Ti] Título:[Method of colored model radicals for assessment of oxidative equilibrium in biologic samples].
[So] Source:Gig Sanit;95(9):884-90, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The most specific method of the recording of the rate offree radical reactions is the method of electron paramagnetic resonance (EPR) spectroscopy, but it is rarely used in applied biology due to expensive equipment and complexity of the execution of measurements. However chemists have found a number of colored organic radicals which lose the coloring under transition into diamagnetic form. In the given paper there are presented results of our studies on the development of methods for the assessment of oxidant equilibrium in biological media with a use of stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) and cation-radicals of N,N-diethyl-p-phenylenediamine (DEPPD). We have developed the new modification of DPPH test, replacing methanol-based incubation medium by non-ionic detergent solution, compatible with native blood serum. Modified DPPH test conserved typical biphasic kinetics of the origin variant, had the similar sensitivity to model antioxidants (IC values 49, 38 and 13 mkMfor ascorbate, a-tocopherol and quercetine, correspondingly) and was applied in experiments on laboratory animals treated with nano- and ionic silver, carbon nanotubes, microfine coal and electrolytic dust. We have tried also the assay of serum lipid hydroperoxides based on Fe-initiated DEPPD oxidation (Alberti et al., 2000). The comparison of kinetics of DEPPD oxidation in model (HO/Fe) and biologic (rat serum/Fe) systems, before and after Fe addition, seems to be an evidence that ceruloplasmin (CP) was involved in the resulting process, but failed to determine its polynomial kinetics, at least for the rat serum and DEPPD excess. The use of CP monoclonal antibodies seems to be the best way for the clarification of the mechanism of this reaction.
[Mh] Termos MeSH primário: Compostos de Bifenilo
Oxirredução
Fenilenodiaminas
Picratos
Plasma
[Mh] Termos MeSH secundário: Animais
Fenômenos Bioquímicos
Compostos de Bifenilo/análise
Compostos de Bifenilo/química
Compostos de Bifenilo/metabolismo
Corantes/análise
Corantes/química
Indicadores e Reagentes/análise
Indicadores e Reagentes/química
Modelos Químicos
Fenilenodiaminas/análise
Fenilenodiaminas/química
Fenilenodiaminas/metabolismo
Picratos/análise
Picratos/química
Picratos/metabolismo
Plasma/química
Plasma/metabolismo
Ratos
Kit de Reagentes para Diagnóstico
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biphenyl Compounds); 0 (Coloring Agents); 0 (Indicators and Reagents); 0 (Phenylenediamines); 0 (Picrates); 0 (Reagent Kits, Diagnostic); 93-05-0 (N,N-diethyl 4-phenylenediamine); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180213
[St] Status:MEDLINE


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[PMID]:29441912
[Au] Autor:Wu JX; Zhang G; Song M; Liu M; Liang JL; Xu JL; Shang F; Qi P
[Ti] Título:Determination of gamithromycin in an injection by ultra-performance liquid chromatography-tandem quadrupole-time-of-flight mass spectrometry.
[So] Source:Pharmazie;71(7):378-381, 2016 Jul 07.
[Is] ISSN:0031-7144
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In this study, a sensitive and precise method was developed for the determination of gamithromycin in an injection using ultra-performance liquid chromatography-tandem quadrupole-time-of-flight mass spectrometry (UPLC-QTOF-MS) and the results were compared with a similar analysis for the ion fragments of gamithromycin in MS/ MS. The sample was dissolved in methanol then filtered and separated on a C18 column using acetonitrile-0.1% formic acid (containing 2 mmol/L ammonium acetate) as the mobile phase. The flow rate was 0.4 mL/min and the column temperature was 40 °C. The mass spectrometry conditions were electrospray ionization (ESI) operated in positive ion full scan mode and quantified using external calibration. Subsequently, ion fragments of the MS/MS were compared and analyzed. The linear range was 10 ∼ 200 µg/L with a correlation coefficient of 0.9992. The limit of detection (LOD) was 0.77 µg/L and the limit of quantitation (LOQ) was 2.55 µg/L. The average recoveries of the intra-assay were 98.8%-105.6% with a relative standard deviation ranging from 1.79% to 2.38% and the inter-assay were 89.3%-110.7% with a relative standard deviation ranging from 4.93% to 6.27%. After the comparative analysis of the fragments with a Molecular Structure Correlator, the score of the total matching degree reached 83.19 and the scores of each ion fragment matching degree were all greater than 90, which supplied the basis for the confirmation of gamithromycin. The results indicated that the method was simple, sensitive and precise and could be applied in the determination of gamithromycin in real samples.
[Mh] Termos MeSH primário: Antibacterianos/análise
Macrolídeos/análise
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão
Indicadores e Reagentes
Limite de Detecção
Padrões de Referência
Reprodutibilidade dos Testes
Espectrometria de Massas por Ionização por Electrospray
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Indicators and Reagents); 0 (Macrolides); ZE856183S0 (gamithromycin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180215
[St] Status:MEDLINE
[do] DOI:10.1691/ph.2016.6004


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[PMID]:29360877
[Au] Autor:Verma V; Kaur C; Grover P; Gupta A; Chaudhary VK
[Ad] Endereço:Centre for Innovation in Infectious Disease Research, Education and Training (CIIDRET), University of Delhi South Campus, New Delhi, India.
[Ti] Título:Biotin-tagged proteins: Reagents for efficient ELISA-based serodiagnosis and phage display-based affinity selection.
[So] Source:PLoS One;13(1):e0191315, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The high-affinity interaction between biotin and streptavidin has opened avenues for using recombinant proteins with site-specific biotinylation to achieve efficient and directional immobilization. The site-specific biotinylation of proteins carrying a 15 amino acid long Biotin Acceptor Peptide tag (BAP; also known as AviTag) is effected on a specific lysine either by co-expressing the E. coli BirA enzyme in vivo or by using purified recombinant E. coli BirA enzyme in the presence of ATP and biotin in vitro. In this paper, we have designed a T7 promoter-lac operator-based expression vector for rapid and efficient cloning, and high-level cytosolic expression of proteins carrying a C-terminal BAP tag in E. coli with TEV protease cleavable N-terminal deca-histidine tag, useful for initial purification. Furthermore, a robust three-step purification pipeline integrated with well-optimized protocols for TEV protease-based H10 tag removal, and recombinant BirA enzyme-based site-specific in vitro biotinylation is described to obtain highly pure biotinylated proteins. Most importantly, the paper demonstrates superior sensitivities in indirect ELISA with directional and efficient immobilization of biotin-tagged proteins on streptavidin-coated surfaces in comparison to passive immobilization. The use of biotin-tagged proteins through specific immobilization also allows more efficient selection of binders from a phage-displayed naïve antibody library. In addition, for both these applications, specific immobilization requires much less amount of protein as compared to passive immobilization and can be easily multiplexed. The simplified strategy described here for the production of highly pure biotin-tagged proteins will find use in numerous applications, including those, which may require immobilization of multiple proteins simultaneously on a solid surface.
[Mh] Termos MeSH primário: Proteínas de Bactérias/isolamento & purificação
Proteínas de Bactérias/metabolismo
Biotina/metabolismo
Ensaio de Imunoadsorção Enzimática/métodos
Biblioteca de Peptídeos
Testes Sorológicos/métodos
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Biotinilação
Carbono-Nitrogênio Ligases/metabolismo
Clonagem Molecular
Citosol/metabolismo
Escherichia coli/citologia
Escherichia coli/genética
Proteínas de Escherichia coli/metabolismo
Vetores Genéticos/genética
Indicadores e Reagentes/metabolismo
Mycobacterium tuberculosis/genética
Proteínas Repressoras/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Escherichia coli Proteins); 0 (Indicators and Reagents); 0 (Peptide Library); 0 (Repressor Proteins); 6SO6U10H04 (Biotin); EC 6.3.- (Carbon-Nitrogen Ligases); EC 6.3.4.15 (birA protein, E coli)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180124
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191315


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[PMID]:28741644
[Au] Autor:Bieszczad B; Gilheany DG
[Ad] Endereço:Centre for Synthesis and Chemical Biology, School of Chemistry, University College Dublin, Belfield, Dublin 4, Ireland. declan.gilheany@ucd.ie.
[Ti] Título:Highly stereoselective construction of the C2 stereocentre of α-tocopherol (vitamin E) by asymmetric addition of Grignard reagents to ketones.
[So] Source:Org Biomol Chem;15(31):6483-6492, 2017 Aug 09.
[Is] ISSN:1477-0539
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Tertiary alcohol precursors of both C2 diastereoisomers of α-tocopherol were prepared in three ways by our recently reported asymmetric Grignard synthesis. The versatility of Grignard chemistry inherent in its three-way disconnection was exploited to allow the synthesis of three product grades: 77 : 23 dr (5 steps), 81 : 19 dr (5 steps) and 96 : 4 dr (7 steps, one gram scale) from readily available and abundant starting materials. The products were converted to their respective α-tocopherols in 3 steps, which allowed a definitive re-assignment of their absolute configurations.
[Mh] Termos MeSH primário: Cetonas/química
Vitaminas/síntese química
alfa-Tocoferol/síntese química
[Mh] Termos MeSH secundário: Álcoois/síntese química
Álcoois/química
Técnicas de Química Sintética
Indicadores e Reagentes
Cetonas/síntese química
Conformação Molecular
Estereoisomerismo
Vitaminas/química
alfa-Tocoferol/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alcohols); 0 (Indicators and Reagents); 0 (Ketones); 0 (Vitamins); H4N855PNZ1 (alpha-Tocopherol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1039/c7ob00751e


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[PMID]:28470518
[Au] Autor:Chen D; Eyupoglu IY; Savaskan N
[Ad] Endereço:Translational Cell Biology and Neurooncology Laboratory of the Universitätsklinikum Erlangen (UKER), Friedrich-Alexander University of Erlangen - Nürnberg (FAU), and Department of Neurosurgery of the Universitätsklinikum Erlangen, Universitätsklinikum Erlangen (UKER), Friedrich-Alexander University
[Ti] Título:Ferroptosis and Cell Death Analysis by Flow Cytometry.
[So] Source:Methods Mol Biol;1601:71-77, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cell death and its recently discovered regulated form ferroptosis are characterized by distinct morphological, electrophysiological, and pharmacological features. In particular ferroptosis can be induced by experimental compounds and clinical drugs (i.e., erastin, sulfasalazine, sorafenib, and artesunate) in various cell types and cancer cells. Pharmacologically, this cell death process can be inhibited by iron chelators and lipid peroxidation inhibitors. Relevance of this specific cell death form has been found in different pathological conditions such as cancer, neurotoxicity, neurodegeneration, and ischemia. Distinguishing cell viability and cell death is essential for experimental and clinical applications and a key component in flow cytometry experiments. Dead cells can compromise the integrity of the data by nonspecific binding of antibodies and dyes. Therefore it is essential that dead cells are robustly and reproducibly identified and characterized by means of cytometry application. Here we describe a procedure to detect and quantify cell death and its specific form ferroptosis based on standard flow cytometry techniques.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Citometria de Fluxo/métodos
Ferro/metabolismo
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/farmacologia
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Dactinomicina/análogos & derivados
Dactinomicina/química
Seres Humanos
Indicadores e Reagentes/química
Necrose
Niacinamida/análogos & derivados
Niacinamida/farmacologia
Compostos de Fenilureia/farmacologia
Piperazinas/farmacologia
Propídio/química
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Indicators and Reagents); 0 (Phenylurea Compounds); 0 (Piperazines); 0 (erastin); 1CC1JFE158 (Dactinomycin); 25X51I8RD4 (Niacinamide); 36015-30-2 (Propidium); 7240-37-1 (7-aminoactinomycin D); 9ZOQ3TZI87 (sorafenib); E1UOL152H7 (Iron)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-6960-9_6


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[PMID]:28470516
[Au] Autor:Ivanov DP; Grabowska AM; Garnett MC
[Ad] Endereço:Cancer Biology, Division of Cancer and Stem Cells, School of Medicine, Queen's Medical Centre, University of Nottingham, Nottingham, NG7 2UH, UK. delyan.ivanov@nottingham.ac.uk.
[Ti] Título:High-Throughput Spheroid Screens Using Volume, Resazurin Reduction, and Acid Phosphatase Activity.
[So] Source:Methods Mol Biol;1601:43-59, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mainstream adoption of physiologically relevant three-dimensional models has been slow in the last 50 years due to long, manual protocols with poor reproducibility, high price, and closed commercial platforms. This chapter describes high-throughput, low-cost, open methods for spheroid viability assessment which use readily available reagents and open-source software to analyze spheroid volume, metabolism, and enzymatic activity. We provide two ImageJ macros for automated spheroid size determination-for both single images and images in stacks. We also share an Excel template spreadsheet allowing users to rapidly process spheroid size data, analyze plate uniformity (such as edge effects and systematic seeding errors), detect outliers, and calculate dose-response. The methods would be useful to researchers in preclinical and translational research planning to move away from simplistic monolayer studies and explore 3D spheroid screens for drug safety and efficacy without substantial investment in money or time.
[Mh] Termos MeSH primário: Sobrevivência Celular
Ensaios de Triagem em Larga Escala/métodos
Indicadores e Reagentes/metabolismo
Esferoides Celulares/fisiologia
[Mh] Termos MeSH secundário: Fosfatase Ácida/metabolismo
Encéfalo/citologia
Linhagem Celular Tumoral
Relação Dose-Resposta a Droga
Avaliação Pré-Clínica de Medicamentos
Ensaios de Triagem em Larga Escala/economia
Seres Humanos
Processamento de Imagem Assistida por Computador
Oxazinas/química
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Software
Esferoides Celulares/citologia
Esferoides Celulares/metabolismo
Fatores de Tempo
Xantenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indicators and Reagents); 0 (Oxazines); 0 (Xanthenes); 1FN9YD6968 (resazurin); EC 3.1.3.2 (Acid Phosphatase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-6960-9_4


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[PMID]:28470513
[Au] Autor:Präbst K; Engelhardt H; Ringgeler S; Hübner H
[Ad] Endereço:Institute of Bioprocess Engineering, Friedrich-Alexander University Erlangen-Nürnberg, Paul-Gordan-Str. 3, 91052, Erlangen, Germany. konstantin.praebst@fau.de.
[Ti] Título:Basic Colorimetric Proliferation Assays: MTT, WST, and Resazurin.
[So] Source:Methods Mol Biol;1601:1-17, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This chapter describes selected assays for the evaluation of cellular viability and proliferation of cell cultures. The underlying principle of these assays is the measurement of a biochemical marker to evaluate the cell's metabolic activity. The formation of the omnipresent reducing agents NADH and NADPH is used as a marker for metabolic activity in the following assays. Using NADH and NADPH as electron sources, specific dyes are biochemically reduced which results in a color change that can be determined with basic photometrical methods. The assays selected for this chapter include MTT, WST, and resazurin. They are applicable for adherent or suspended cell lines, easy to perform, and comparably economical. Detailed protocols and notes for easier handling and avoiding pitfalls are enclosed to each assay.
[Mh] Termos MeSH primário: Contagem de Células/métodos
Proliferação Celular
Sobrevivência Celular
Colorimetria/métodos
Indicadores e Reagentes/química
Oxazinas/química
Sais de Tetrazólio/química
Tiazóis/química
Xantenos/química
[Mh] Termos MeSH secundário: Bioensaio
Calibragem
Células HeLa
Seres Humanos
NAD/análise
NADP/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H tetrazolium monosodium salt); 0 (Indicators and Reagents); 0 (Oxazines); 0 (Tetrazolium Salts); 0 (Thiazoles); 0 (Xanthenes); 0U46U6E8UK (NAD); 1FN9YD6968 (resazurin); 53-59-8 (NADP); EUY85H477I (thiazolyl blue)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-6960-9_1


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[PMID]:28461658
[Au] Autor:Kohl TO; Ascoli CA
[Ti] Título:Indirect Immunometric ELISA.
[So] Source:Cold Spring Harb Protoc;2017(5):pdb.prot093708, 2017 May 01.
[Is] ISSN:1559-6095
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This assay facilitates the immunometric determination of assay-associated reagents including the capture and detection antibodies as well as the analyte (i.e., antigen or antibody). It can precede the development of a sandwich enzyme-linked immunosorbent assay (ELISA) in which optimal antibody concentrations are applied for the quantitative measurement of the antigen. This protocol describes the materials and equipment required for the measurement of chromogenic substrate development; however, it can be adapted for use with chemiluminescent- and fluorescent-labeled reporters.
[Mh] Termos MeSH primário: Ensaio de Imunoadsorção Enzimática/métodos
[Mh] Termos MeSH secundário: Anticorpos/análise
Antígenos/análise
Soros Imunes/análise
Indicadores e Reagentes/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies); 0 (Antigens); 0 (Immune Sera); 0 (Indicators and Reagents)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1101/pdb.prot093708


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[PMID]:28461656
[Au] Autor:Behringer R; Gertsenstein M; Nagy KV; Nagy A
[Ti] Título:Shipment of Live Preimplantation-Stage Mouse Embryos.
[So] Source:Cold Spring Harb Protoc;2017(5):pdb.prot092742, 2017 May 01.
[Is] ISSN:1559-6095
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sharing genetically modified mouse models is a very important part of collaboration between researchers. Shipping live animals around the world is inconvenient, expensive, and cumbersome because of the variety of international regulations and paperwork. The issue of health status differences between animal facilities is of great importance; traditionally, imported animals are quarantined to determine their health status and avoid the introduction of undesirable pathogens. The shipment of preimplantation-stage embryos for immediate transfer into pseudopregnant recipients upon arrival is a commonly used method for transportation. Time coordination on both sides is critical in this case, but the shipment can be done by any courier and the container does not need to be returned. This protocol has been used since the early 1990s to rederive dozens of mouse strains.
[Mh] Termos MeSH primário: Blastocisto/fisiologia
Camundongos/embriologia
Transportes/métodos
[Mh] Termos MeSH secundário: Animais
Transferência Embrionária
Feminino
Indicadores e Reagentes
Pseudogravidez
Transportes/instrumentação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indicators and Reagents)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1101/pdb.prot092742


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[PMID]:28461655
[Au] Autor:Mata J; Wise JA
[Ad] Endereço:Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, United Kingdom jm593@cam.ac.uk.
[Ti] Título:4-Thiouridine Labeling to Analyze mRNA Turnover in .
[So] Source:Cold Spring Harb Protoc;2017(5):pdb.prot091645, 2017 May 01.
[Is] ISSN:1559-6095
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Traditionally, the half-lives of mRNAs were measured after inhibition of transcription to allow decay of the preexisting population. The protocol presented here is a more recently developed strategy in which mRNA turnover is analyzed by measuring the decline in levels of newly synthesized RNA labeled with 4-thiouridine (4sU) during a brief pulse. After RNA extraction, the 4sU is biotinylated and the labeled species are purified using streptavidin beads. DNA microarrays can then be used to compare this population with total RNA, allowing half-lives to be calculated.
[Mh] Termos MeSH primário: Marcadores de Afinidade
RNA Fúngico/metabolismo
RNA Mensageiro/metabolismo
Schizosaccharomyces/metabolismo
Tiouridina
[Mh] Termos MeSH secundário: Antimetabólitos
Biotinilação
Meia-Vida
Indicadores e Reagentes
Análise de Sequência com Séries de Oligonucleotídeos
RNA Fúngico/biossíntese
RNA Mensageiro/análise
RNA Mensageiro/biossíntese
Estreptavidina
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Affinity Labels); 0 (Antimetabolites); 0 (Indicators and Reagents); 0 (RNA, Fungal); 0 (RNA, Messenger); 13957-31-8 (Thiouridine); 9013-20-1 (Streptavidin)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1101/pdb.prot091645



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