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[PMID]:29267507
[Au] Autor:Liu N; Ding D; Wang L; Zhao H; Zhu L; Geng X
[Ad] Endereço:Department of Critical Care Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.
[Ti] Título:Two novel Mg(II)-based and Zn(II)-based complexes: inhibiting growth of human liver cancer cells.
[So] Source:Braz J Med Biol Res;51(2):e6929, 2017 Dec 18.
[Is] ISSN:1414-431X
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Two new Mg(II)-based and Zn(II)-based coordination polymers, {[Mg3(BTB)(DMA)4](DMA)2}n (1, H3BTB=1,3,5-benzenetrisbenzoic acid, DMA=N,N-dimethylacetamide) and {(H2NMe2)2[Zn3(BTB)2(OH)(Im)](DMF)9(MeOH)7}n (2, Im=imidazole, DMF=N,N-dimethylformamide), have been successfully synthesized and structurally characterized under solvothermal conditions. 1 contains a linear [Mg3(COO)6] cluster that connected by the fully deprotonated BTB3- ligands to give a kgd-type 2D bilayer structure; 2 represents a microporous 3D pillar-layered system based on the binuclear Zn units and pillared Im ligands, which shows a (3,5)-connected hms topological net. In addition, in vitro anticancer activities of compounds 1 and 2 on 4 human liver cancer cells (HB611, HHCC, BEL-7405 and SMMC-7721) were determined.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Benzimidazóis/farmacologia
Neoplasias Hepáticas/tratamento farmacológico
Estruturas Metalorgânicas/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos/síntese química
Benzimidazóis/síntese química
Linhagem Celular Tumoral
Seres Humanos
Ligantes
Neoplasias Hepáticas/patologia
Magnésio/química
Estruturas Metalorgânicas/síntese química
Estrutura Molecular
Zinco/química
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Benzimidazoles); 0 (Ligands); 0 (Metal-Organic Frameworks); I38ZP9992A (Magnesium); J41CSQ7QDS (Zinc)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE


  2 / 86388 MEDLINE  
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[PMID]:28453850
[Au] Autor:Mukherjee S; Mukherjee S; Maiti TK; Bhattacharya S; Sinha Babu SP
[Ad] Endereço:Department of Zoology (Centre for Advanced Studies), Visva-Bharati University, West Bengal, India.
[Ti] Título:A Novel Ligand of Toll-like Receptor 4 From the Sheath of Wuchereria bancrofti Microfilaria Induces Proinflammatory Response in Macrophages.
[So] Source:J Infect Dis;215(6):954-965, 2017 03 15.
[Is] ISSN:1537-6613
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Background: Lymphatic filariasis, frequently caused from Wuchereria bancrofti infection, is endemic in several parts of the globe and responsible for human health problems and socioeconomic loss to a large extent. Inflammatory consequences originating from host-parasite interaction play a major role in the disease pathology and allied complications. The identity of the key mediator of this process is yet unknown in filarial research. Methods: Microfilarial protein (MfP) was isolated from the sheath of W. bancrofti microfilariae through ultrafiltration, followed by chromatographic separation. Expression of signaling molecules was studied by enzyme-linked immunosorbent assay and immunoblotting. Binding of MfP to Toll-like receptor 4 (TLR4) was determined by co-immunoprecipitation, fluorescein isothiocyanate-probing, and surface plasmon resonance analysis. Results: We found that MfP (approximately 70 kDa) binds to macrophage-TLR4 and triggers nuclear factor kappa beta activation that upregulates secretion of proinflammatory cytokines. Microfilarial protein failed to induce inflammation in either TLRKO macrophage or macrophage treated with TLR4 inhibitor, indicating that MfP acts through TLR4. We have also detected phenotypic transformation of macrophages from anti-inflammatory (M2) to proinflammatory (M1) subtype after incubation with MfP. Conclusions: Microfilarial protein appears to be a new ligand of TLR4 from W. bancrofti. Determination of its functional attributions in the host-parasite relationship, especially immunopathogenesis of filarial infection, may improve our understanding.
[Mh] Termos MeSH primário: Antígenos de Helmintos/imunologia
Proteínas de Helminto/imunologia
Macrófagos/imunologia
Receptor 4 Toll-Like/imunologia
Wuchereria bancrofti/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Anti-Helmínticos/sangue
Células Cultivadas
Ensaio de Imunoadsorção Enzimática
Células HEK293
Interações Hospedeiro-Parasita
Seres Humanos
Ligantes
Camundongos
Camundongos Endogâmicos BALB C
Microfilárias/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Helminth); 0 (Antigens, Helminth); 0 (Helminth Proteins); 0 (Ligands); 0 (Toll-Like Receptor 4)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1093/infdis/jix067


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[PMID]:29302024
[Au] Autor:Chen Y; Ho JML; Shis DL; Gupta C; Long J; Wagner DS; Ott W; Josic K; Bennett MR
[Ad] Endereço:Department of Biosciences, Rice University, 6100 Main Street, Houston, TX, 77005, USA.
[Ti] Título:Tuning the dynamic range of bacterial promoters regulated by ligand-inducible transcription factors.
[So] Source:Nat Commun;9(1):64, 2018 01 04.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:One challenge for synthetic biologists is the predictable tuning of genetic circuit regulatory components to elicit desired outputs. Gene expression driven by ligand-inducible transcription factor systems must exhibit the correct ON and OFF characteristics: appropriate activation and leakiness in the presence and absence of inducer, respectively. However, the dynamic range of a promoter (i.e., absolute difference between ON and OFF states) is difficult to control. We report a method that tunes the dynamic range of ligand-inducible promoters to achieve desired ON and OFF characteristics. We build combinatorial sets of AraC-and LasR-regulated promoters containing -10 and -35 sites from synthetic and Escherichia coli promoters. Four sequence combinations with diverse dynamic ranges were chosen to build multi-input transcriptional logic gates regulated by two and three ligand-inducible transcription factors (LacI, TetR, AraC, XylS, RhlR, LasR, and LuxR). This work enables predictable control over the dynamic range of regulatory components.
[Mh] Termos MeSH primário: Proteínas de Escherichia coli/genética
Escherichia coli/genética
Regulação Bacteriana da Expressão Gênica
Regiões Promotoras Genéticas/genética
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Algoritmos
Escherichia coli/metabolismo
Proteínas de Escherichia coli/metabolismo
Ligantes
Modelos Genéticos
Termodinâmica
Fatores de Transcrição/metabolismo
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Escherichia coli Proteins); 0 (Ligands); 0 (Transcription Factors)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02473-5


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[PMID]:28985538
[Au] Autor:Chillè D; Foti C; Giuffrè O
[Ad] Endereço:Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, Università di Messina, 98166 Messina, Italy.
[Ti] Título:Thermodynamic parameters for the protonation and the interaction of arsenate with Mg , Ca and Sr : Application to natural waters.
[So] Source:Chemosphere;190:72-79, 2018 Jan.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Thermodynamic parameters for the protonation of AsO and for the interaction with Mg , Ca and Sr were reported, comprehensive also of their dependence on ionic strength, considering the 0.1 ≤ I ≤ 1 M range and using NaCl as background salt. The same speciation models were obtained for Mg , Ca and Sr systems, with the formation of three different species: ML, MLH and MLH (L = AsO ). Mono- and di-protonated species were very weak, with formation constant values (log K) ranging from 1.45 to 3.23. In order to have a complete picture of thermodynamic properties of the systems under study and to fill the shortage of thermodynamic data on arsenate complex systems, the ligand protonation and metal complex enthalpies were also determined by calorimetric titrations, at t = 25 °C and in NaCl at I = 0.7 M (for H -AsO species also at I = 0.1 M). On the light of the proposed speciation models, examples of As(V) distribution in some natural waters are reported.
[Mh] Termos MeSH primário: Arseniatos/química
Concentração Osmolar
Termodinâmica
Água/química
[Mh] Termos MeSH secundário: Cálcio/química
Calorimetria
Ligantes
Magnésio/química
Cloreto de Sódio/química
Estrôncio/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arsenates); 0 (Ligands); 059QF0KO0R (Water); 451W47IQ8X (Sodium Chloride); I38ZP9992A (Magnesium); SY7Q814VUP (Calcium); YZS2RPE8LE (Strontium)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171007
[St] Status:MEDLINE


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[PMID]:28460457
[Au] Autor:Trivedi T; Zheng Y; Fournier PGJ; Murthy S; John S; Schillo S; Dunstan CR; Mohammad KS; Zhou H; Seibel MJ; Guise TA
[Ad] Endereço:Bone Research Program, ANZAC Research Institute, University of Sydney, Sydney, Australia.
[Ti] Título:The vitamin D receptor is involved in the regulation of human breast cancer cell growth via a ligand-independent function in cytoplasm.
[So] Source:Oncotarget;8(16):26687-26701, 2017 Apr 18.
[Is] ISSN:1949-2553
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Vitamin D has pleiotropic effects on multiple tissues, including malignant tumors. Vitamin D inhibits breast cancer growth through activation of the vitamin D receptor (VDR) and via classical nuclear signaling pathways. Here, we demonstrate that the VDR can also function in the absence of its ligand to control behaviour of human breast cancer cells both outside and within the bone microenvironment. Stable shRNA expression was used to knock down VDR expression in MCF-7 cells, generating two VDR knockdown clonal lines. In ligand-free culture, knockdown of VDR in MCF-7 cells significantly reduced proliferation and increased apoptosis, suggesting that the VDR plays a ligand-independent role in cancer cell growth. Implantation of these VDR knockdown cells into the mammary fat pad of nude mice resulted in reduced tumor growth in vivo compared with controls. In the intra-tibial xenograft model, VDR knockdown greatly reduced the ability of the cells to form tumors in the bone microenvironment. The in vitro growth of VDR knockdown cells was rescued by the expression of a mutant form of VDR which is unable to translocate to the nucleus and hence accumulates in the cytoplasm. Thus, our data indicate that in the absence of ligand, the VDR promotes breast cancer growth both in vitro and in vivo and that cytoplasmic accumulation of VDR is sufficient to produce this effect in vitro. This new mechanism of VDR action in breast cancer cells contrasts the known anti-proliferative nuclear actions of the VDR-vitamin D ligand complex.
[Mh] Termos MeSH primário: Neoplasias da Mama/metabolismo
Receptores de Calcitriol/metabolismo
[Mh] Termos MeSH secundário: Animais
Apoptose/genética
Neoplasias Ósseas/genética
Neoplasias Ósseas/metabolismo
Neoplasias Ósseas/patologia
Neoplasias da Mama/genética
Linhagem Celular Tumoral
Proliferação Celular
Citoplasma/metabolismo
Modelos Animais de Doenças
Feminino
Expressão Gênica
Técnicas de Silenciamento de Genes
Xenoenxertos
Seres Humanos
Ligantes
Camundongos
Mutação
Osteosclerose/genética
Osteosclerose/metabolismo
Transporte Proteico
Receptores de Calcitriol/genética
Vitamina D/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ligands); 0 (Receptors, Calcitriol); 0 (VDR protein, human); 1406-16-2 (Vitamin D)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.18632/oncotarget.15803


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[PMID]:28460447
[Au] Autor:Dou X; Han J; Song W; Dong N; Xu X; Zhang W; Shan A
[Ad] Endereço:Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, P.R. China.
[Ti] Título:Sodium butyrate improves porcine host defense peptide expression and relieves the inflammatory response upon Toll-like receptor 2 activation and histone deacetylase inhibition in porcine kidney cells.
[So] Source:Oncotarget;8(16):26532-26551, 2017 Apr 18.
[Is] ISSN:1949-2553
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Host defense peptides (HDPs) are an important component of the innate immune system and possess direct antimicrobial and immunomodulatory activities. Dietary regulation of HDPs synthesis has emerged as a novel non-antibiotic approach to combat pathogen infection. There are species- and tissue-dependent characteristics of the regulation and mechanism of HDPs. In this study, we investigated whether the histone deacetylase inhibitor (HDACi) sodium butyrate (NaB) could induce HDP expression and the mechanism underlying NaB-regulated HDP expression in PK-15 cells. Our results revealed that NaB augmented HDP expression in PK-15 cells, including porcine ß-defensin 3 (pBD3), epididymis protein 2 splicing variant C (pEP2C), pBD128, pBD123, and pBD115, but no inflammatory response occurred. Inhibition of HDAC activity was not sufficient to induce the expression of pBD3 and pEP2C in comparisons of NaB and another HDACi, trichostatin A (TSA). Concomitantly, NF-κB activation was involved in the induction of HDP expression by NaB. MAPK pathway inhibition also prevented pBD3 and pEP2C induction by NaB. Furthermore, NaB could still promote pBD3 and pEP2C expression and inhibit IL-6 production in the presence of the toll-like receptor 2 (TLR2) ligand peptidoglycan. Moreover, TLR2 could be activated by both NaB and peptidoglycan, and blocking TLR2 expression suppressed HDP induction. Finally, we further showed that increased pBD3 could decrease cytokine interleukin-18 (IL-18) and increase porcine claudin 15 (pCLDN15) contents, suggesting an immunoregulatory function of pBD3. In conclusion, this work paves the way for using HDACi-NaB to induce porcine kidney defense peptides while limiting the deleterious risk of an inflammatory response.
[Mh] Termos MeSH primário: Ácido Butírico/farmacologia
Defensinas/genética
Células Epiteliais/efeitos dos fármacos
Células Epiteliais/metabolismo
Regulação da Expressão Gênica/efeitos dos fármacos
Inibidores de Histona Desacetilases/farmacologia
Receptor 2 Toll-Like/metabolismo
[Mh] Termos MeSH secundário: Animais
Peptídeos Catiônicos Antimicrobianos/genética
Linhagem Celular
Células Cultivadas
Citocinas/genética
Citocinas/metabolismo
Mediadores da Inflamação
Rim
Ligantes
NF-kappa B/metabolismo
Transdução de Sinais/efeitos dos fármacos
Suínos
Receptor 2 Toll-Like/agonistas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antimicrobial Cationic Peptides); 0 (Cytokines); 0 (Defensins); 0 (Histone Deacetylase Inhibitors); 0 (Inflammation Mediators); 0 (Ligands); 0 (NF-kappa B); 0 (Toll-Like Receptor 2); 107-92-6 (Butyric Acid)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.18632/oncotarget.15714


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[PMID]:29407590
[Au] Autor:Cooper AG; MacDonald C; Glass M; Hook S; Tyndall JDA; Vernall AJ
[Ad] Endereço:School of Pharmacy, University of Otago, Dunedin, New Zealand.
[Ti] Título:Alkyl indole-based cannabinoid type 2 receptor tools: Exploration of linker and fluorophore attachment.
[So] Source:Eur J Med Chem;145:770-789, 2018 Feb 10.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Cannabinoid type 2 (CB ) receptor continues to emerge as a promising drug target for many diseases and conditions. New tools for studying CB receptor are required to further inform how this receptor functions in healthy and diseased states. The alkyl indole scaffold is a well-recognised ligand for cannabinoid receptors, and in this study the indole C5-7 positions were explored for linker and fluorophore attachment. A new high affinity, CB receptor selective inverse agonist was identified (16b) along with a general trend of C5-substituted indoles acting as agonists versus C7-substituted indoles acting as inverse agonists. The indole C7 position was found to be the most tolerant to linker extension and resulted in a high affinity inverse agonist with a medium length linker (19). Although a high affinity fluorescent ligand for CB receptor was not identified in this study, the indole C7 position shows great promise for fluorophore or probe attachment.
[Mh] Termos MeSH primário: Corantes Fluorescentes/farmacologia
Indóis/farmacologia
Receptor CB2 de Canabinoide/agonistas
[Mh] Termos MeSH secundário: Relação Dose-Resposta a Droga
Corantes Fluorescentes/síntese química
Corantes Fluorescentes/química
Seres Humanos
Indóis/síntese química
Indóis/química
Ligantes
Modelos Moleculares
Estrutura Molecular
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fluorescent Dyes); 0 (Indoles); 0 (Ligands); 0 (Receptor, Cannabinoid, CB2); 8724FJW4M5 (indole)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180207
[St] Status:MEDLINE


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[PMID]:29331752
[Au] Autor:Reddy TS; Privér SH; Mirzadeh N; Bhargava SK
[Ad] Endereço:Centre for Advanced Materials & Industrial Chemistry (CAMIC), School of Science, RMIT University, GPO BOX 2476, Melbourne 3001, Australia.
[Ti] Título:Synthesis of gold(I) phosphine complexes containing the 2-BrC F PPh ligand: Evaluation of anticancer activity in 2D and 3D spheroidal models of HeLa cancer cells.
[So] Source:Eur J Med Chem;145:291-301, 2018 Feb 10.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Newly synthesised mononuclear gold complexes containing the 2-BrC F PPh ligand have been fully characterised and their anticancer activity towards five human tumor [prostate (PC3), glioblastoma (U87MG), cervical (HeLa), fibrosarcoma (HT1080), ovarian (SKOV-3)] and normal human embryonic kidney (Hek-293T) cell lines investigated. Some of the synthesised gold complexes displayed higher cytotoxicity than cisplatin towards PC-3, HeLa and U87MG cells and inhibited the thioredoxin reductase (TrxR) enzyme, which is considered a potential target for new compounds in cancer treatment. The more physiologically relevant tumor spheroid assay demonstrated the superior potency of these gold phosphine complexes in inhibiting the growth of cervical carcinoma cell line HeLa (3D) spheroidal models. The mechanism of cell death was shown to be apoptotic cell death through cell cycle arrest, mitochondrial membrane depolarisation and increased ROS production.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Modelos Biológicos
Compostos Organoáuricos/farmacologia
Fosfinas/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos/síntese química
Antineoplásicos/química
Morte Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Células Cultivadas
Cristalografia por Raios X
Relação Dose-Resposta a Droga
Ensaios de Seleção de Medicamentos Antitumorais
Células HeLa
Seres Humanos
Ligantes
Modelos Moleculares
Estrutura Molecular
Compostos Organoáuricos/síntese química
Compostos Organoáuricos/química
Fosfinas/química
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2-BrC6F4PPh2); 0 (Antineoplastic Agents); 0 (Ligands); 0 (Organogold Compounds); 0 (Phosphines); FW6947296I (phosphine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180115
[St] Status:MEDLINE


  9 / 86388 MEDLINE  
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[PMID]:29291444
[Au] Autor:Khodadust F; Ahmadpour S; Aligholikhamseh N; Abedi SM; Hosseinimehr SJ
[Ad] Endereço:Department of Radiopharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
[Ti] Título:An improved Tc-HYNIC-(Ser) -LTVSPWY peptide with EDDA/tricine as co-ligands for targeting and imaging of HER2 overexpression tumor.
[So] Source:Eur J Med Chem;144:767-773, 2018 Jan 20.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Overexpression of human epidermal receptor 2 (HER2) has given the opportunity for targeting and delivering of imaging radiotracers. The aim of this study was to evaluate the Tc-HYNIC-(EDDA/tricine)-(Ser) -LTVSPWY peptide for tumor targeting and imaging of tumor with overexpression of HER2. The HYNIC-(Ser) -LTVSPWY was labeled with Tc in presence of EDDA/tricine mixture as co-ligands. The in vitro and in vivo studies of this radiolabeled peptide were performed for cellular specific binding and tumor targeting. The high radiochemical purity of Tc-HYNIC (EDDA/tricine)-(Ser) -LTVSPWY was obtained to be 99%. It exhibited high stability in normal saline and human serum. In HER2 binding affinity study, a significant reduction in uptake of radiolabeled peptide (7.7 fold) was observed by blocking SKOV-3 cells receptors with unlabeled peptide. The K and B values for this radiolabeled peptide were determined as 3.3 ±â€¯1.0 nM and 2.9 ±â€¯0.3 × 10 CPM/pMol, respectively. Biodistribution study revealed tumor to blood and tumor to muscle ratios about 6.9 and 4 respectively after 4 h. Tumor imaging by gamma camera demonstrated considerable high contrast tumor uptake. This developed Tc-HYNIC-(Ser) -LTVSPWY peptide selectively targeted on HER2 tumor and exhibited a high target uptake combined with acceptable low background activity for tumor imaging in mice. The results of this study and its comparison with another study showed that Tc-HYNIC-(EDDA/tricine)-(Ser) -LTVSPWY is much better than previously reported radiolabeled peptide as Tc-CSSS-LTVSPWY for HER2 overexpression tumor targeting and imaging.
[Mh] Termos MeSH primário: Glicina/análogos & derivados
Neoplasias/diagnóstico
Oligopeptídeos/farmacocinética
Compostos de Organotecnécio/farmacocinética
Receptor ErbB-2/biossíntese
[Mh] Termos MeSH secundário: Animais
Linhagem Celular Tumoral
Relação Dose-Resposta a Droga
Feminino
Glicina/química
Glicina/farmacocinética
Seres Humanos
Ligantes
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Nus
Estrutura Molecular
Neoplasias/metabolismo
Neoplasias Experimentais/diagnóstico
Neoplasias Experimentais/metabolismo
Oligopeptídeos/síntese química
Oligopeptídeos/química
Compostos de Organotecnécio/síntese química
Compostos de Organotecnécio/química
Relação Estrutura-Atividade
Distribuição Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 ((99m)Tc-HYNIC-(Ser)3-LTVSPWY); 0 (Ligands); 0 (Oligopeptides); 0 (Organotechnetium Compounds); EC 2.7.10.1 (ERBB2 protein, human); EC 2.7.10.1 (Receptor, ErbB-2); TE7660XO1C (Glycine); W12LH4V8V3 (tricine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180102
[St] Status:MEDLINE


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[PMID]:29188999
[Au] Autor:Song LC; Zhu L; Hu FQ; Wang YX
[Ad] Endereço:Department of Chemistry, State Key Laboratory of Elemento-Organic Chemistry, College of Chemistry, and ‡Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University , Tianjin 300071, China.
[Ti] Título:Studies on Chemical Reactivity and Electrocatalysis of Two Acylmethyl(hydroxymethyl)pyridine Ligand-Containing [Fe]-Hydrogenase Models (2-COCH -6-HOCH C H N)Fe(CO) L (L = η -SCOMe, η -2-SC H N).
[So] Source:Inorg Chem;56(24):15216-15230, 2017 Dec 18.
[Is] ISSN:1520-510X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:On the basis of preparation and characterization of [Fe]-H ase models (2-COCH -6-HOCH C H N)Fe(CO) L (A, L = η -SCOMe; B, L = η -2-SC H N), the chemical reactivities of A and B with various electrophilic and nucleophilic reagents have been investigated, systematically. Thus, when A reacted with 1 equiv of MeCOCl in the presence of Et N in MeCN to give the η -SCOMe-coordinated acylation product (2-COCH -6-MeCO CH C H N)Fe(CO) (η -SCOMe) (1), treatment of A with excess HBF ·Et O in MeCN gave the cationic MeCN-coordinated complex [(2-COCH -6-HOCH C H N)Fe(CO) (MeCN)](BF ) (2). In addition, when 2 was treated with 1 equiv of 2,6-(p-4-MeC H ) C H SK or PPh in CH Cl to give the thiophenolato- and PPh -substituted derivatives (2-COCH -6-HOCH C H N)Fe(CO) [2,6-(p-MeC H ) C H S] (3) and [(2-COCH -6-HOCH C H N)Fe(CO) (PPh )](BF ) (4), treatment of B with 1 equiv of PMe or P(OMe) in THF afforded the phosphine- and phosphite-substituted complexes (2-COCH -6-HOCH C H N)(η -2-SC H N)Fe(CO) L (5, L = PMe ; 6, L = P(OMe) ). Interestingly, in contrast to A, when B reacted with excess HBF ·Et O in MeCN to afford the BF adduct [2-COCH -6-HO(BF )CH C H N]Fe(CO) (η -2-SC H N) (7), reaction of B with 1 equiv of p-MeC H COCl in the presence of Et N in MeCN gave not only the expected 2-acylmethyl-6-p-toluoyloxomethylpyridine-containing complex (2-COCH -6-p-MeC H CO CH C H N)Fe(CO) (η -2-SC H N) (8), but also gave the unexpected 2-toluoyloxovinyl-6-toluoyloxomethylpyridine-containing complex (2-p-MeC H CO C H-6-p-MeC H CO CH C H N)Fe(CO) (η -2-SC H N) (9). While the possible pathways for the novel reactions leading to complexes 1, 2, and 7-9 are suggested, the structures of complexes B, 1-4, and 6-9 were unambiguously confirmed by X-ray crystallography. In addition, model complexes A and B have been found to be catalysts for proton reduction to H from TFA under CV conditions.
[Mh] Termos MeSH primário: Materiais Biomiméticos/química
Hidrogenase/química
Compostos de Ferro/química
Proteínas com Ferro-Enxofre/química
Piridinas/química
[Mh] Termos MeSH secundário: Catálise
Cristalografia por Raios X
Técnicas Eletroquímicas
Ligantes
Modelos Moleculares
Oxirredução
Prótons
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Iron Compounds); 0 (Iron-Sulfur Proteins); 0 (Ligands); 0 (Protons); 0 (Pyridines); 0 (acylmethyl(hydroxymethyl)pyridine); EC 1.12.- (iron hydrogenase); EC 1.12.7.2 (Hydrogenase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE
[do] DOI:10.1021/acs.inorgchem.7b02582



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