Base de dados : MEDLINE
Pesquisa : D27.888.569.864 [Categoria DeCS]
Referências encontradas : 7289 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 729 ir para página                         

  1 / 7289 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28457675
[Au] Autor:Wichgers Schreur PJ; van Keulen L; Kant J; Kortekaas J
[Ad] Endereço:Department of Virology, Wageningen Bioveterinary Research, Lelystad, The Netherlands. Electronic address: paul.wichgersschreur@wur.nl.
[Ti] Título:Four-segmented Rift Valley fever virus-based vaccines can be applied safely in ewes during pregnancy.
[So] Source:Vaccine;35(23):3123-3128, 2017 05 25.
[Is] ISSN:1873-2518
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Rift Valley fever virus (RVFV) causes severe and recurrent outbreaks on the African continent and the Arabian Peninsula and continues to expand its habitat. This mosquito-borne virus, belonging to the genus Phlebovirus of the family Bunyaviridae contains a tri-segmented negative-strand RNA genome. Previously, we developed four-segmented RVFV (RVFV-4s) variants by splitting the M-genome segment into two M-type segments each encoding one of the structural glycoproteins; Gn or Gc. Vaccination/challenge experiments with mice and lambs subsequently showed that RVFV-4s induces protective immunity against wild-type virus infection after a single administration. To demonstrate the unprecedented safety of RVFV-4s, we here report that the virus does not cause encephalitis after intranasal inoculation of mice. A study with pregnant ewes subsequently revealed that RVFV-4s does not cause viremia and does not cross the ovine placental barrier, as evidenced by the absence of teratogenic effects and virus in the blood and organs of the fetuses. Altogether, these results show that the RVFV-4s vaccine virus can be applied safely in pregnant ewes.
[Mh] Termos MeSH primário: Febre do Vale de Rift/prevenção & controle
Vírus da Febre do Vale do Rift/genética
Vírus da Febre do Vale do Rift/imunologia
Doenças dos Ovinos/prevenção & controle
Vacinas Virais/administração & dosagem
Vacinas Virais/efeitos adversos
[Mh] Termos MeSH secundário: Animais
Anticorpos Neutralizantes/sangue
Anticorpos Antivirais/sangue
Encefalite Viral/etiologia
Encefalite Viral/veterinária
Feminino
Genoma Viral/genética
Genoma Viral/imunologia
Camundongos
Gravidez
Febre do Vale de Rift/virologia
Vírus da Febre do Vale do Rift/química
Ovinos
Carneiro Doméstico/imunologia
Teratogênios
Vacinas Atenuadas/administração & dosagem
Vacinas Atenuadas/efeitos adversos
Vacinas Atenuadas/imunologia
Vacinas Virais/imunologia
Viremia/prevenção & controle
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Neutralizing); 0 (Antibodies, Viral); 0 (Teratogens); 0 (Vaccines, Attenuated); 0 (Viral Vaccines)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


  2 / 7289 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29181896
[Au] Autor:Yang X; Li S; Wang Z; Lee SMY; Wang LH; Wang R
[Ad] Endereço:State Key Laboratory of Quality Research in Chinese Medicine, and Institute of Chinese Medical Sciences, University of Macau, Avenida da Universidade, Taipa, Macau, China.
[Ti] Título:Constraining the Teratogenicity of Pesticide Pollution by a Synthetic Nanoreceptor.
[So] Source:Chem Asian J;13(1):41-45, 2018 Jan 04.
[Is] ISSN:1861-471X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The teratogenicity of the pesticide nereistoxin (NTX) and its derivative thiocyclam (THI) towards aquatic life was dramatically constrained by a synthetic nanoreceptor, cucurbit[7]uril, through selective encapsulation of the pesticides (K of 3.24(±0.31)×10 m and K of 7.46(±0.10)×10 m ), as evidenced by the rate of hatchability, morphology development, and tyrosinase activity of zebrafish larvae incubated with the pesticides (3-300 µm) in the absence and in the presence of 300 µm cucurbit[7]uril, demonstrating the significant potential of the nanoreceptor in managing ecological pollution of these pesticides.
[Mh] Termos MeSH primário: Hidrocarbonetos Aromáticos com Pontes/farmacologia
Compostos Heterocíclicos com 1 Anel/antagonistas & inibidores
Compostos Heterocíclicos com 1 Anel/toxicidade
Imidazóis/farmacologia
Toxinas Marinhas/antagonistas & inibidores
Toxinas Marinhas/toxicidade
Teratogênios/toxicidade
Poluentes Químicos da Água/antagonistas & inibidores
[Mh] Termos MeSH secundário: Animais
Hidrocarbonetos Aromáticos com Pontes/química
Relação Dose-Resposta a Droga
Compostos Heterocíclicos com 1 Anel/química
Imidazóis/química
Larva/efeitos dos fármacos
Toxinas Marinhas/química
Estrutura Molecular
Relação Estrutura-Atividade
Teratogênios/química
Poluentes Químicos da Água/química
Poluentes Químicos da Água/toxicidade
Peixe-Zebra
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bridged-Ring Compounds); 0 (Heterocyclic Compounds, 1-Ring); 0 (Imidazoles); 0 (Marine Toxins); 0 (N,N-dimethyl-1,2,3-trithian-5-yl amine); 0 (Teratogens); 0 (Water Pollutants, Chemical); 0 (cucurbit(7)uril); 1631-58-9 (nereistoxin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE
[do] DOI:10.1002/asia.201701527


  3 / 7289 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29337257
[Au] Autor:Dimopoulou M; Verhoef A; Gomes CA; van Dongen CW; Rietjens IMCM; Piersma AH; van Ravenzwaay B
[Ad] Endereço:Division of Toxicology, Wageningen University, The Netherlands; National Institute of Public Health and the Environment (RIVM), Bilthoven, The Netherlands. Electronic address: myrto.dimopoulou@wur.nl.
[Ti] Título:A comparison of the embryonic stem cell test and whole embryo culture assay combined with the BeWo placental passage model for predicting the embryotoxicity of azoles.
[So] Source:Toxicol Lett;286:10-21, 2018 Apr.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In the present study, we show the value of combining toxico-dynamic and -kinetic in vitro approaches for embryotoxicity testing of azoles. Both the whole embryo culture (WEC) and the embryonic stem cells test (EST) predicted the in vivo potency ranking of twelve tested azoles with moderate accuracy. Combining these results with relative placental transfer rates (Papp values) as determined in the BeWo cell culture model, increased the predictability of both WEC and EST, with R values increasing from 0.51 to 0.87 and from 0.35 to 0.60, respectively. The comparison of these in vitro systems correlated well (R = 0.67), correctly identifying the in vivo strong and weak embryotoxicants. Evaluating also specific gene responses related with the retinoic acid and sterol biosynthesis pathways, which represent the toxicological and fungicidal mode of action of azoles respectively in the WEC and EST, we observed that the differential regulation of Dhrs3 and Msmo1 reached higher magnitudes in both systems compared to Cyp26a1 and Cyp51. Establishing sensitive biomarkers across the in vitro systems for studying the underlying mechanism of action of chemicals, such as azoles, is valuable for comparing alternative in vitro models and for improving insight in the mechanism of developmental toxicity of chemicals.
[Mh] Termos MeSH primário: Azóis/toxicidade
Bioensaio
Embrião de Mamíferos/efeitos dos fármacos
Células-Tronco Embrionárias Murinas/efeitos dos fármacos
Placenta/efeitos dos fármacos
Teratogênios/toxicidade
Testes de Toxicidade/métodos
[Mh] Termos MeSH secundário: Oxirredutases do Álcool/genética
Oxirredutases do Álcool/metabolismo
Animais
Transporte Biológico
Diferenciação Celular/efeitos dos fármacos
Linhagem Celular
Relação Dose-Resposta a Droga
Técnicas de Cultura Embrionária
Embrião de Mamíferos/metabolismo
Embrião de Mamíferos/patologia
Feminino
Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos
Seres Humanos
Cinética
Camundongos
Oxigenases de Função Mista/genética
Oxigenases de Função Mista/metabolismo
Células-Tronco Embrionárias Murinas/metabolismo
Células-Tronco Embrionárias Murinas/patologia
Miócitos Cardíacos/efeitos dos fármacos
Miócitos Cardíacos/metabolismo
Miócitos Cardíacos/patologia
Placenta/metabolismo
Placenta/patologia
Gravidez
Reprodutibilidade dos Testes
Ácido Retinoico 4 Hidroxilase/genética
Ácido Retinoico 4 Hidroxilase/metabolismo
Medição de Risco
Esterol 14-Desmetilase/genética
Esterol 14-Desmetilase/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Azoles); 0 (Teratogens); EC 1.- (Mixed Function Oxygenases); EC 1.1.- (Alcohol Oxidoreductases); EC 1.1.1.71 (DHRS3 protein, mouse); EC 1.14.13.70 (Cyp51 protein, mouse); EC 1.14.13.70 (Sterol 14-Demethylase); EC 1.14.13.72 (methylsterol monooxygenase); EC 1.14.14.1 (Cyp26a1 protein, mouse); EC 1.14.14.1 (Retinoic Acid 4-Hydroxylase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE


  4 / 7289 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28745489
[Au] Autor:Wani TH; Chakrabarty A; Shibata N; Yamazaki H; Guengerich FP; Chowdhury G
[Ad] Endereço:Departments of Chemistry and Life Sciences, SONS, Shiv Nadar University , Greater Noida, Uttar Pradesh 201314, India.
[Ti] Título:The Dihydroxy Metabolite of the Teratogen Thalidomide Causes Oxidative DNA Damage.
[So] Source:Chem Res Toxicol;30(8):1622-1628, 2017 08 21.
[Is] ISSN:1520-5010
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Thalidomide [α-(N-phthalimido)glutarimide] (1) is a sedative and antiemetic drug originally introduced into the clinic in the 1950s for the treatment of morning sickness. Although marketed as entirely safe, more than 10 000 babies were born with severe birth defects. Thalidomide was banned and subsequently approved for the treatment of multiple myeloma and complications associated with leprosy. Although known for more than 5 decades, the mechanism of teratogenicity remains to be conclusively understood. Various theories have been proposed in the literature including DNA damage and ROS and inhibition of angiogenesis and cereblon. All of the theories have their merits and limitations. Although the recently proposed cereblon theory has gained wide acceptance, it fails to explain the metabolism and low-dose requirement reported by a number of groups. Recently, we have provided convincing structural evidence in support of the presence of arene oxide and the quinone-reactive intermediates. However, the ability of these reactive intermediates to impart toxicity/teratogenicity needs investigation. Herein we report that the oxidative metabolite of thalidomide, dihydroxythalidomide, is responsible for generating ROS and causing DNA damage. We show, using cell lines, the formation of comet (DNA damage) and ROS. Using DNA-cleavage assays, we also show that catalase, radical scavengers, and desferal are capable of inhibiting DNA damage. A mechanism of teratogenicity is proposed that not only explains the DNA-damaging property but also the metabolism, low concentration, and species-specificity requirements of thalidomide.
[Mh] Termos MeSH primário: Dano ao DNA/efeitos dos fármacos
Talidomida/toxicidade
[Mh] Termos MeSH secundário: Catalase/metabolismo
Clivagem do DNA
Depuradores de Radicais Livres/química
Células HEK293
Células Hep G2
Células Endoteliais da Veia Umbilical Humana
Seres Humanos
Microscopia de Fluorescência
Plasmídeos/metabolismo
Poli(ADP-Ribose) Polimerases/metabolismo
Espécies Reativas de Oxigênio/análise
Espécies Reativas de Oxigênio/metabolismo
Teratogênios/química
Teratogênios/metabolismo
Teratogênios/toxicidade
Talidomida/química
Talidomida/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Free Radical Scavengers); 0 (Reactive Oxygen Species); 0 (Teratogens); 4Z8R6ORS6L (Thalidomide); EC 1.11.1.6 (Catalase); EC 2.4.2.30 (Poly(ADP-ribose) Polymerases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180127
[Lr] Data última revisão:
180127
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1021/acs.chemrestox.7b00127


  5 / 7289 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28892742
[Au] Autor:Karthik R; Manigandan V; Saravanan R
[Ad] Endereço:Department of Medical Biotechnology, Chettinad Academy of Research and Education, Kelambakkam, Chennai-603 103, Tamil Nadu, India.
[Ti] Título:Toxicity, teratogenicity and antibacterial activity of posterior salivary gland (PSG) toxin from the cuttlefish Sepia pharaonis (Ehrenberg, 1831).
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1064:28-35, 2017 Oct 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Toxins from the posterior salivary gland (PSG) of cuttlefishes are known toxins with pronounced toxicity. In the present study, ionic peptide rich PSG toxin from the cuttlefish S. pharaonis was isolated by ion exchange chromatography and purified by Reversed Phase High Performance Liquid Chromatography (RP-HPLC), with active fraction at a retention time of 26min. The net protein content of the PSG toxin was estimated to be 46.6mg at a proximate molecular weight of∼50kDa. Fourier Transform Infrared Spectroscopy (FT-IR) of PSG toxin revealed the presence of alcoholic OH, primary NH, alkyl CH and conjugated CONH functional groups. Circular Dichroism (CD) spectroscopy and K2D analysis of the PSG toxin confirmed the presence of secondary structure with 36.77% α-helix,12.31% ß sheet and 50.92% random coil. Scanning Electron Microscopy (SEM) of the PSG toxin eluted amberlite IRA 900 Cl resin showed surface abrasion and corrosive blebbing. Energy Dispersive X-ray Spectrometry (EDX) analysis of PSG toxin treated resin revealed increase in nitrogen and sulphur content corresponding to amino acid composition. Teratogenicity of PSG toxin against Zebrafish embryo demonstrated developmental malformations and premature hatching at a maximum tolerated dose of 1.25µM. The PSG toxin (50µM) exhibited commendable inhibitory activity with pronounced zone of inhibition against gram E. coli (10mm) and K. pneumonia (10mm). The results strongly demonstrate the toxicity of the ionic peptide rich PSG toxin from S. pharaonis and its exploitation for its promise as a potential antibacterial agent of the future.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Toxinas Marinhas/farmacologia
Toxinas Marinhas/toxicidade
Sepia/química
Teratogênios/toxicidade
[Mh] Termos MeSH secundário: Animais
Antibacterianos/química
Bactérias/efeitos dos fármacos
Embrião não Mamífero/efeitos dos fármacos
Embrião não Mamífero/patologia
Toxinas Marinhas/química
Toxinas Marinhas/isolamento & purificação
Teratogênios/química
Peixe-Zebra
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Marine Toxins); 0 (Teratogens)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170912
[St] Status:MEDLINE


  6 / 7289 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28822238
[Au] Autor:Zota AR; Shamasunder B
[Ad] Endereço:Department of Environmental and Occupational Health, George Washington University Milken Institute School of Public Health, Washington DC. Electronic address: azota@gwu.edu.
[Ti] Título:The environmental injustice of beauty: framing chemical exposures from beauty products as a health disparities concern.
[So] Source:Am J Obstet Gynecol;217(4):418.e1-418.e6, 2017 Oct.
[Is] ISSN:1097-6868
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The obstetrics-gynecology community has issued a call to action to prevent toxic environmental chemical exposures and their threats to healthy human reproduction. Recent committee opinions recognize that vulnerable and underserved women may be impacted disproportionately by environmental chemical exposures and recommend that reproductive health professionals champion policies that secure environmental justice. Beauty product use is an understudied source of environmental chemical exposures. Beauty products can include reproductive and developmental toxicants such as phthalates and heavy metals; however, disclosure requirements are limited and inconsistent. Compared with white women, women of color have higher levels of beauty product-related environmental chemicals in their bodies, independent of socioeconomic status. Even small exposures to toxic chemicals during critical periods of development (such as pregnancy) can trigger adverse health consequences (such as impacts on fertility and pregnancy, neurodevelopment, and cancer). In this commentary, we seek to highlight the connections between environmental justice and beauty product-related chemical exposures. We describe racial/ethnic differences in beauty product use (such as skin lighteners, hair straighteners, and feminine hygiene products) and the potential chemical exposures and health risks that are associated with these products. We also discuss how targeted advertising can take advantage of mainstream beauty norms to influence the use of these products. Reproductive health professionals can use this information to advance environmental justice by being prepared to counsel patients who have questions about toxic environmental exposures from beauty care products and other sources. Researchers and healthcare providers can also promote health-protective policies such as improved ingredient testing and disclosure for the beauty product industry. Future clinical and public health research should consider beauty product use as a factor that may shape health inequities in women's reproductive health across the life course.
[Mh] Termos MeSH primário: Cosméticos/efeitos adversos
[Mh] Termos MeSH secundário: Carcinógenos/toxicidade
Doenças do Sistema Endócrino/induzido quimicamente
Feminino
Disparidades nos Níveis de Saúde
Seres Humanos
Gravidez
Resultado da Gravidez
Teratogênios/toxicidade
Saúde da Mulher
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carcinogens); 0 (Cosmetics); 0 (Teratogens)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170820
[St] Status:MEDLINE


  7 / 7289 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28763493
[Au] Autor:Tokunaga E; Akiyama H; Soloshonok VA; Inoue Y; Hara H; Shibata N
[Ad] Endereço:Department of Nanopharmaceutical Sciences, Nagoya Institute of Technology, Nagoya, Japan.
[Ti] Título:Biological evaluation of both enantiomers of fluoro-thalidomide using human myeloma cell line H929 and others.
[So] Source:PLoS One;12(8):e0182152, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Over the last few years, thalidomide has become one of the most important anti-tumour drugs for the treatment of relapsed-refractory multiple myeloma. However, besides its undesirable teratogenic side effect, its configurational instability critically limits any further therapeutic improvements of this drug. In 1999, we developed fluoro-thalidomide which is a bioisostere of thalidomide, but, in sharp contrast to the latter, it is configurationally stable and readily available in both enantiomeric forms. The biological activity of fluoro-thalidomide however, still remains virtually unstudied, with the exception that fluoro-thalidomide is not teratogenic. Herein, we report the first biological evaluation of fluoro-thalidomide in racemic and in both (R)- and (S)-enantiomerically pure forms against (in vitro) H929 cells of multiple myeloma (MM) using an annexin V assay. We demonstrate that all fluoro-thalidomides inhibited the growth of H929 MM cells without any in-vivo activation. Furthermore, we report that the enantiomeric forms of fluoro-thalidomide display different anti-tumour activities, with the (S)-enantiomer being noticeably more potent. The angiogenesis of fluoro-thalidomides is also investigated and compared to thalidomide. The data obtained in this study paves the way towards novel pharmaceutical research on fluoro-thalidomides.
[Mh] Termos MeSH primário: Inibidores da Angiogênese/farmacologia
Antineoplásicos/uso terapêutico
Ensaios de Seleção de Medicamentos Antitumorais
Mieloma Múltiplo/patologia
Talidomida/análogos & derivados
Talidomida/farmacologia
[Mh] Termos MeSH secundário: Apoptose
Inibidores de Caspase/química
Caspases/metabolismo
Ciclo Celular
Linhagem Celular
Linhagem Celular Tumoral
Fármacos Dermatológicos/uso terapêutico
Células Endoteliais da Veia Umbilical Humana
Seres Humanos
Espectroscopia de Ressonância Magnética
Mieloma Múltiplo/tratamento farmacológico
Neovascularização Patológica
Proteínas Proto-Oncogênicas c-bcl-2/metabolismo
Estereoisomerismo
Teratogênios
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Angiogenesis Inhibitors); 0 (Antineoplastic Agents); 0 (BCL2 protein, human); 0 (Caspase Inhibitors); 0 (Dermatologic Agents); 0 (Proto-Oncogene Proteins c-bcl-2); 0 (Teratogens); 4Z8R6ORS6L (Thalidomide); EC 3.4.22.- (Caspases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170802
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182152


  8 / 7289 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28716577
[Au] Autor:Félix LM; Serafim C; Valentim AM; Antunes LM; Matos M; Coimbra AM
[Ad] Endereço:Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal; Institute for Research and Innovation in Health (i3S), University of Porto (UP), Porto, Portugal; Laboratory Animal Science (LAS), In
[Ti] Título:Apoptosis-related genes induced in response to ketamine during early life stages of zebrafish.
[So] Source:Toxicol Lett;279:1-8, 2017 Sep 05.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Increasing evidence supports that ketamine, a widely used anaesthetic, potentiates apoptosis during development through the mitochondrial pathway of apoptosis. Defects in the apoptotic machinery can cause or contribute to the developmental abnormalities previously described in ketamine-exposed zebrafish. The involvement of the apoptotic machinery in ketamine-induced teratogenicity was addressed by assessing the apoptotic signals at 8 and 24 hpf following 20min exposure to ketamine at three stages of early zebrafish embryo development (256 cell, 50% epiboly and 1-4 somites stages). Exposure at the 256-cell stage to ketamine induced an up-regulation of casp8 and pcna at 8 hpf while changes in pcna at the mRNA level were observed at 24 hpf. After the 50% epiboly stage exposure, the mRNA levels of casp9 were increased at 8 and 24 hpf while aifm1 was affected at 24 hpf. Both tp53 and pcna expressions were increased at 8 hpf. After exposure during the 1-4 somites stage, no meaningful changes on transcript levels were observed. The distribution of apoptotic cells and the caspase-like enzymatic activities of caspase-3 and -9 were not affected by ketamine exposure. It is proposed that ketamine exposure at the 256-cell stage induced a cooperative mechanism between proliferation and cellular death while following exposure at the 50% epiboly, a p53-dependent and -independent caspase activation may occur. Finally, at the 1-4 somites stage, the defence mechanisms are already fully in place to protect against ketamine-insult. Thus, ketamine teratogenicity seems to be dependent on the functional mechanisms present in each developmental stage.
[Mh] Termos MeSH primário: Anestésicos Dissociativos/toxicidade
Proteínas Reguladoras de Apoptose/genética
Apoptose/efeitos dos fármacos
Blástula/efeitos dos fármacos
Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos
Ketamina/toxicidade
Teratogênios/toxicidade
Proteínas de Peixe-Zebra/genética
Peixe-Zebra/genética
[Mh] Termos MeSH secundário: Animais
Apoptose/genética
Proteínas Reguladoras de Apoptose/metabolismo
Blástula/metabolismo
Blástula/patologia
Proliferação Celular/efeitos dos fármacos
Relação Dose-Resposta a Droga
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Medição de Risco
Fatores de Tempo
Proteína Supressora de Tumor p53/genética
Proteína Supressora de Tumor p53/metabolismo
Peixe-Zebra/embriologia
Peixe-Zebra/metabolismo
Proteínas de Peixe-Zebra/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anesthetics, Dissociative); 0 (Apoptosis Regulatory Proteins); 0 (RNA, Messenger); 0 (Teratogens); 0 (Tumor Suppressor Protein p53); 0 (Zebrafish Proteins); 690G0D6V8H (Ketamine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170719
[St] Status:MEDLINE


  9 / 7289 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28689802
[Au] Autor:Zhu J; Hu L; Li L; Huang X; Shi H
[Ad] Endereço:State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai 200062, China.
[Ti] Título:Comparison of phenotypic and global gene expression changes in Xenopus tropicalis embryos induced by agonists of RAR and RXR.
[So] Source:Toxicol Appl Pharmacol;330:40-47, 2017 Sep 01.
[Is] ISSN:1096-0333
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Retinoic acid functions through two classes of receptors, i.e., the retinoic acid receptor (RAR) and the retinoid X receptor (RXR). The difference in the role between RAR and RXR, however, are not well clarified. In the present study, we comparatively investigated the phenotypic and global gene expression changes in Xenopus tropicalis embryos induced by three different agonists, including a RAR selective ligand (all-trans retinoic acid, at-RA), a RXR selective ligand (fluorobexarotene, FBA) and their common ligand (9-cis retinoic acid, 9c-RA). All three agonists induced striking and similar malformations in X. tropicalis embryos at the concentrations of 5-50µg/L. Especially, the development of anterior structures and caudal region was dramatically altered. The hierarchical clustering analysis of phenotypes and gene profiles suggested that effects induced by 9c-RA separated from those by at-RA and FBA. The up-regulated genes were involved in multiple pathways while down-regulated genes were mainly related to phototransduction and tyrosine metabolism. at-RA primarily affected the retinol, glycolysis, starch and sucrose metabolisms while FBA led to disturbances in more wide-ranging pathways such as the PPAR, adipocytokine, insulin, FoxO signaling pathways, alanine, aspartate and glutamate metabolism. RXR is a heterodimeric partner for several other nuclear receptors, which opens the possibility that additional retinoid effects could be mediated by FBA, such as RXR-PPAR. Our data indicates that not only RXR-RAR but also RXR-PPAR plays important roles in the control of metabolism with retinoid treatment in X. tropicalis embryos.
[Mh] Termos MeSH primário: Expressão Gênica/efeitos dos fármacos
Receptores do Ácido Retinoico/agonistas
Receptores X Retinoide/agonistas
Xenopus/genética
Xenopus/metabolismo
[Mh] Termos MeSH secundário: Animais
Embrião não Mamífero/anatomia & histologia
Embrião não Mamífero/efeitos dos fármacos
Transdução de Sinal Luminoso/efeitos dos fármacos
Transdução de Sinal Luminoso/genética
Análise em Microsséries
Fenótipo
Teratogênios/toxicidade
Tretinoína/farmacologia
Tirosina/metabolismo
Vitamina A/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, Retinoic Acid); 0 (Retinoid X Receptors); 0 (Teratogens); 11103-57-4 (Vitamin A); 42HK56048U (Tyrosine); 5688UTC01R (Tretinoin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170711
[St] Status:MEDLINE


  10 / 7289 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28646619
[Au] Autor:Jungmann P; Pires P; Araujo Júnior E
[Ad] Endereço:Department of Pathology, Pernambuco University, Recife, Brazil.
[Ti] Título:Early insights into Zika's microcephaly physiopathology from the epicenter of the outbreak: teratogenic apoptosis in the central nervous system.
[So] Source:Acta Obstet Gynecol Scand;96(9):1039-1044, 2017 Sep.
[Is] ISSN:1600-0412
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fetal infection by the Zika virus has been implicated in the exceptional rise in the number of microcephalic newborns recorded by the end of 2015 in Brazil. The mechanism by which this teratogenic effect is produced in the developing brain has not been fully established. Very early in the outbreak, we addressed this question by evaluating available initial data from a gestational and postnatal clinical investigation in the Brazilian state of Pernambuco. The present study was undertaken to test the hypothesis that the subtractive dysmorphic brain malformations observed in Zika-related microcephaly are primarily due to the massive induction of apoptosis of neuroprogenitor cells. We designed a physiopathological algorithm based on the examination of the following medical findings: epidemiological data, ultrasound images, computed tomography scans, placental tissue, cerebral fluid analysis, eye fundoscopy, neurological examination and necroscopy findings.
[Mh] Termos MeSH primário: Surtos de Doenças
Microcefalia/epidemiologia
Complicações Infecciosas na Gravidez
Infecção pelo Zika virus
Zika virus/patogenicidade
[Mh] Termos MeSH secundário: Apoptose
Brasil/epidemiologia
Sistema Nervoso Central/patologia
Sistema Nervoso Central/virologia
Feminino
Seres Humanos
Recém-Nascido
Microcefalia/fisiopatologia
Gravidez
Teratogênios/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Teratogens)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170625
[St] Status:MEDLINE
[do] DOI:10.1111/aogs.13184



página 1 de 729 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde