| [PMID]: | 29429161 |
| [Au] Autor: | Li L; Duan XJ; Sun Y; Lu Y; Xu HY; Wang QZ; Wang HY |
| [Ad] Endereço: | Department of Pathology, Fuwai Hospital, Chinese Academy of Medical Sciences, Beijing 100037, China. |
| [Ti] Título: | [Classification of cardiac amyloidosis: an immunohistochemical analysis]. |
| [So] Source: | Zhonghua Bing Li Xue Za Zhi;47(2):105-109, 2018 Feb 08. |
| [Is] ISSN: | 0529-5807 |
| [Cp] País de publicação: | China |
| [La] Idioma: | chi |
| [Ab] Resumo: | To evaluate the sensitivity and specificity of immunohistochemistry (IHC) in the classification of cardiac amyloidosis on endomyocardial biopsy (EMB) and heart allograft. Twenty cardiac tissues from 19 patients at Fuwai Hospital from January, 1990 to April, 2017 with histopathologic features of amyloidosis and Congo red staining positivity were included. IHC was performed with monoclonal antibodies against AA amyloid and polyclonal antibodies against transthyretin (ATTR), λ-light chain (AL-λ), κ-light chain (AL-κ), ApoAâ… , ApoAâ…¡, ApoA â…£ and ß(2)-microglobin. The extent of interstitial staining was evaluated by light microscopy, and three patterns were recognized; these included diffuse pericellular pattern, discrete pericellular pattern, and nodular pattern. Two patterns of vascular deposition were also noted, including arterial pattern and venous pattern. Endocardial involvement was also assessed and recorded. Nineteen cases were divided into three groups according to the pattern of proteins expression in specimens. The first group (5 cases) only showed single protein expression on EMB. The second group (6 cases) showed more than one protein expression, but one of them was intensely stained or any staining of any protein together with ApoA â…£ co-staining. The third group (8 cases) also showed more than one protein expression and all of them had intense staining. Amyloid deposits were successfully subtyped as AL-λ, ATTR, AL-κ and ApoAâ… by IHC in the former two groups with the sensitivity of 11/19. In the third group, amyloid deposits could not be subtyped by immunohistochemistry due to their poor specificity. The pericellular pattern tended to favor AL over ATTR amyloidosis and vascular deposition tended to favor ATTR. Amyloid deposits can be reliably subtyped in diagnostic cardiac specimens using IHC. The co-deposition of chaperon proteins, the distribution of amyloid proteins and clinical features are also auxiliary to subtype cardiac amyloidosis. |
| [Mh] Termos MeSH primário: |
Amiloidose/patologia
Cardiomiopatias/patologia
|
| [Mh] Termos MeSH secundário: |
Amiloide/análise
Neuropatias Amiloides Familiares/patologia
Anticorpos Monoclonais/análise
Apolipoproteína A-I/análise
Apolipoproteínas A/análise
Biópsia
Seres Humanos
Cadeias kappa de Imunoglobulina/análise
Cadeias lambda de Imunoglobulina/análise
Imuno-Histoquímica
Placa Amiloide/patologia
Sensibilidade e Especificidade
|
| [Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (APOA1 protein, human); 0 (Amyloid); 0 (Antibodies, Monoclonal); 0 (Apolipoprotein A-I); 0 (Apolipoproteins A); 0 (Immunoglobulin kappa-Chains); 0 (Immunoglobulin lambda-Chains); 0 (apolipoprotein A-IV) |
| [Em] Mês de entrada: | 1803 |
| [Cu] Atualização por classe: | 180306 |
[Lr] Data última revisão:
| 180306 |
| [Sb] Subgrupo de revista: | IM |
| [Da] Data de entrada para processamento: | 180213 |
| [St] Status: | MEDLINE |
| [do] DOI: | 10.3760/cma.j.issn.0529-5807.2018.02.005 |
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