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  1 / 5317 MEDLINE  
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[PMID]:29431926
[Au] Autor:Kalaev VN; Nechaeva MS
[Ti] Título:[The study of the frequency of buccal epithelium cells with nuclear in athletes in dependence on the place got in the competition].
[So] Source:Gig Sanit;95(10):992-7, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Cytogenetic homeostasis ofathletes involved in hand-to-hand armyfighting, depending on the outcome of the fight and the level of aggressiveness has been studied. Status of the genetic apparatus in athletes was evaluated in buccal epithelium with the use of micronucleus test, which is widely usedfor the determination of the influence of various factors on the genetic stability of the organism. Psychological testing of athletes was executed with the use of Spielberger's State-Trait Anger Expression Inventory scale, Bass-Darky questionnaire for the diagnostics of aggression and hostile reactions, Eysenck Personality Questionnaire test for the evaluation ofpsychic conditions and method of U.P. Ilyin and P.A. Kovalev for the identification of the propensity to conflictness and aggressiveness (positive and negative) as the personal characteristics. The place taken by the athlete on the competition was shown to have an impact on the frequency of buccal epithelium cells with nuclear abnormalities (micronuclei, perinuclear vacuoles, notches, protrusions "tongue" and "broken egg"). Losers have nuclear aberrations more than winners. At that in athletes with higher aggressiveness and its attendant psychological characteristics the influence of the outcome of the competition on the number of cells with aberrations core is more pronounced.
[Mh] Termos MeSH primário: Agressão
Desempenho Atlético
Artes Marciais
Testes para Micronúcleos/métodos
Mucosa Bucal/patologia
[Mh] Termos MeSH secundário: Adolescente
Agressão/fisiologia
Agressão/psicologia
Atletas/psicologia
Desempenho Atlético/fisiologia
Desempenho Atlético/psicologia
Análise Citogenética/métodos
Seres Humanos
Masculino
Artes Marciais/fisiologia
Artes Marciais/psicologia
Testes Psicológicos
Estatística como Assunto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180213
[St] Status:MEDLINE


  2 / 5317 MEDLINE  
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[PMID]:29431322
[Au] Autor:Shinkaruk EV; Agbalyan EV
[Ti] Título:[Cytogenetic status of the residents of the Gydansky Peninsula (Gydan)].
[So] Source:Gig Sanit;95(9):865-8, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The relevance of the study on the Gydansky Peninsula lies in poor knowledge and inaccessibility of the territory, planned intensive industrial development of the Gydansky Peninsula, in 2011 there were received licenses for the exploration of license areas at the peninsula up to 2031. Industrial development will inevitably lead to certain environmental shifts, emission of the harmful substances into biosphere, the accumulation of anthropogenic pollutants in soil and water sources. The proposed development of the territory of the Peninsula Gydan sets the task of assessment of the impact of gas and oil production in conditions of the far North on health, as well indigenous persons as employees recruited to this of the region. One of the informative approaches to the assessment of population health is the assessment of the cytogenetic status with the use of noninvasive analysis of buccal epithelium. The aim of the study is to determine the cytogenetic status of the inhabitants of the village of Antipayuta of the Yamal-Nenets Autonomous Okrug for the assessment of the impact of environmental factors on the health of the population at the present stage of the industrial development of the territory. Samples of buccal epithelium of 81 alien and indigenous people of the Yamal-Nenets Autonomous district were the object of the investigation. There was performed the analysis of indices of cells of buccal epithelium of the residents living in the village in comparison with the control group. The analysis of samples was performed on a Nikon Eclipse E100 microscope. For the assessment of the cytogenetic status of the individual there was used the proposed by Sycheva L. P. (2012-Index of accumulation of cytogenetic damages (Iac). It is shown that the frequency of occurrence of micronucleus and nuclear protrusions does not exceed the performance of the control group. The index of accumulation of cytogenetic damage for the population of the village is 0.78±0.07% and corresponds to a low level. The analysis of indices of buccal epithelial cells of residents living in rural areas was performed in the comparison with the control group The prevalence rate of cells with micronuclei and protrusions were shown to account of 14.6% andfail to exceed indices in the control group. Accumulation index of cytogenetic damages for the village population is 0.78 ± 0.07 % and corresponds to the low level. The amount of cells with micronuclei and protrusions in the current study is by 14.6% lower than the average of the control group. The intensity of proliferative processes and apoptosis in the comparison groups occurs evenly.
[Mh] Termos MeSH primário: Análise Citogenética
Exposição Ambiental
Desenvolvimento Industrial/tendências
Saúde Pública
Regionalização/organização & administração
[Mh] Termos MeSH secundário: Regiões Árticas/epidemiologia
Análise Citogenética/métodos
Análise Citogenética/estatística & dados numéricos
Exposição Ambiental/análise
Exposição Ambiental/prevenção & controle
Exposição Ambiental/normas
Implementação de Plano de Saúde/métodos
Implementação de Plano de Saúde/tendências
Seres Humanos
Saúde da População/estatística & dados numéricos
Saúde Pública/normas
Saúde Pública/tendências
Federação Russa/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180213
[St] Status:MEDLINE


  3 / 5317 MEDLINE  
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[PMID]:29430903
[Au] Autor:Sycheva LP; Budarina OV; Sabirova ZF; Akhaltseva LV; Rosolovsky AP
[Ti] Título:[Cytogenetic status of children in the hygienic assessment of the air pollution by odorous substances].
[So] Source:Gig Sanit;95(8):765-8, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:For the first time the impact of odorous emissions from the enterprise for the production of chewing gum on the cytogenetic status of children, residing in the distance of 0,9 and 6 km, was studied. Specific weak mint and fruity odour in the air in the placement of the enterprise may be related to the presence of menthol, menthone, pinene, limonene, ethyl acetate and other compounds containing in the emissions. There were no differences in cytogenetic status of children in both groups (by frequency of micronuclei, protrusions, binucleated cells, apoptosis).
[Mh] Termos MeSH primário: Poluentes Atmosféricos
Mucosa Bucal/patologia
Odorantes
[Mh] Termos MeSH secundário: Poluentes Atmosféricos/efeitos adversos
Poluentes Atmosféricos/análise
Poluentes Atmosféricos/classificação
Pré-Escolar
Análise Citogenética/métodos
Análise Citogenética/estatística & dados numéricos
Exposição Ambiental/efeitos adversos
Exposição Ambiental/prevenção & controle
Feminino
Seres Humanos
Masculino
Indústria Manufatureira/normas
Odorantes/análise
Odorantes/prevenção & controle
Estatística como Assunto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Air Pollutants)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180213
[St] Status:MEDLINE


  4 / 5317 MEDLINE  
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[PMID]:29412553
[Au] Autor:Ladnova GG; Istomin AV; Kurochitskaya MG; Silyutina VV
[Ti] Título:[Cytogenetic indices of buccal epithelium in schoolchildren residing in territories with different levels of the air pollution].
[So] Source:Gig Sanit;95(5):428-31, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:In the article there are contained the results of the study of ambient air of the regional center, urban-type settlement, and buccal epithelium in 87 schoolchildren aged of 11-13 years. Schoolchildren residing in the regional center were shown to be more susceptible to the exposure to such toxicants as carbon monoxide, nitrogen oxide, fluorhydric acid, chlorine and its compounds, sulfur dioxide, formaldehyde contained in the ambient air. In this group of students if compared with students who live in the village, there were revealed more pronounced cytogenetic deteriorations in oral mucosal epitheliocytes: cells with micronuclei, different forms of protrusion of the core, dual-core cell mitosis, kariokynesis, apoptotic bodies significantly more common occur, that can speak about both not only of cytogenetic instability, but and the degree of environmental genotoxicity, in particular, ambient air. There was noted the tendency to increase the level of cytogenetic damage and destruction of the nucleus in the group of girls in comparison with boys.
[Mh] Termos MeSH primário: Substâncias Perigosas
Mucosa Bucal/patologia
[Mh] Termos MeSH secundário: Adolescente
Poluentes Atmosféricos/efeitos adversos
Poluentes Atmosféricos/análise
Criança
Análise Citogenética/métodos
Exposição Ambiental/prevenção & controle
Monitoramento Ambiental/métodos
Monitoramento Ambiental/estatística & dados numéricos
Feminino
Substâncias Perigosas/efeitos adversos
Substâncias Perigosas/análise
Seres Humanos
Masculino
Determinação de Necessidades de Cuidados de Saúde
Poluentes Radioativos/efeitos adversos
Poluentes Radioativos/análise
Federação Russa/epidemiologia
Serviços de Saúde Escolar/estatística & dados numéricos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Air Pollutants); 0 (Hazardous Substances); 0 (Radioactive Pollutants)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180208
[St] Status:MEDLINE


  5 / 5317 MEDLINE  
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[PMID]:29265181
[Au] Autor:Horai M; Satoh S; Matsuo M; Iwanaga M; Horio K; Jo T; Takasaki Y; Kawaguchi Y; Tsushima H; Yoshida S; Taguchi M; Itonaga H; Sawayama Y; Taguchi J; Imaizumi Y; Hata T; Moriuchi Y; Haase D; Yoshiura KI; Miyazaki Y
[Ad] Endereço:Department of Haematology, Atomic Bomb Disease and Hibakusha Medicine Unit, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
[Ti] Título:Chromosomal analysis of myelodysplastic syndromes among atomic bomb survivors in Nagasaki.
[So] Source:Br J Haematol;180(3):381-390, 2018 02.
[Is] ISSN:1365-2141
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The myelodysplastic syndromes (MDS) are clonal haematopoietic disorders that develop de novo and also secondary to chemotherapy and/or radiation therapy. We previously demonstrated that the risk of MDS is increased among atomic bomb survivors with significant correlation to radiation dose; however, the clinical characteristics of these survivors have not been well analysed. In this study, we investigated chromosomal abnormalities of MDS among survivors. The frequency of abnormal karyotypes was significantly higher, with more very poor risk karyotypes, according to the revised International Prognostic Scoring System, among those exposed close to the hypocentre compared with unexposed cases. However, abnormal karyotype frequency did not reflect the prognosis of exposed cases with respect to distance from the hypocentre. In addition, there was no difference in prognosis between exposed and unexposed cases. Among proximally exposed cases (<1·5 km from the hypocentre), chromosomal translocations and inversions were more frequent, and the frequency of structural alterations in chromosomes 3, 8, and 11 was significantly increased compared with unexposed cases. These results suggest that chromosomal alterations in MDS among survivors have different features compared with those in de novo or therapy-related MDS. Detailed molecular study is warranted.
[Mh] Termos MeSH primário: Aberrações Cromossômicas
Vítimas de Desastres
Síndromes Mielodisplásicas/epidemiologia
Síndromes Mielodisplásicas/genética
Armas Nucleares
Sobreviventes
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Contagem de Células Sanguíneas
Medula Óssea/patologia
Análise Citogenética
Feminino
Seres Humanos
Japão/epidemiologia
Masculino
Meia-Idade
Síndromes Mielodisplásicas/diagnóstico
Síndromes Mielodisplásicas/terapia
Avaliação de Resultados da Assistência ao Paciente
Sistema de Registros
Análise de Sobrevida
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1111/bjh.15050


  6 / 5317 MEDLINE  
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[PMID]:29377069
[Au] Autor:Scharenberg C; Jansson M; Saft L; Hellström-Lindberg E
[Ad] Endereço:Department of Medicine, Centre for Haematology and Regenerative Medicine, Karolinska University Hospital Huddinge, Karolinska Institutet, Stockholm, Sweden.
[Ti] Título:Megakaryocytes harbour the del(5q) abnormality despite complete clinical and cytogenetic remission induced by lenalidomide treatment.
[So] Source:Br J Haematol;180(4):526-533, 2018 02.
[Is] ISSN:1365-2141
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The mechanisms underlying lenalidomide-resistance of del(5q) MDS stem cells remain to be elucidated and may include cell-intrinsic as well as microenvironmental causes. Abnormal hypolobated megakaryocytes constitute one of the hallmarks of del(5q) MDS. We hypothesized that these cells have potential implications for the regulation of haematopoietic stem cells (HSC) similarly to what has recently been described for megakaryocytes in the murine system. Therefore, we conducted a study to determine the response of abnormal hypolobated megakaryocytes to lenalidomide therapy. We studied lenalidomide-treated patients in the MDS-004 trial as well as a cohort seen at our institution. Morphological evaluation at time of complete cytogenetic remission (CCyR) demonstrated the persistence of hypolobated megakaryocytes in all evaluable patients (n = 9). Furthermore, we provide evidence that the abnormal hypolobated morphology is restricted to del(5q) megakaryocytes, both at diagnosis and during CCyR. Using fluorescence in situ hybridisation analysis on flow-sorted stem- and progenitor populations, we observed a similar degree of clonal involvement in megakaryocyte-erythroid-progenitors as in HSC. Taken together, our findings suggest that megakaryocyte morphology might aid in the evaluation of patients where discontinuation of lenalidomide is considered and offers interesting hypotheses for further investigation of lenalidomide resistance.
[Mh] Termos MeSH primário: Antineoplásicos/uso terapêutico
Deleção Cromossômica
Cromossomos Humanos Par 5
Neoplasias Hematológicas/tratamento farmacológico
Neoplasias Hematológicas/genética
Megacariócitos/metabolismo
Talidomida/análogos & derivados
[Mh] Termos MeSH secundário: Antineoplásicos/administração & dosagem
Antineoplásicos/efeitos adversos
Medula Óssea/patologia
Evolução Clonal
Análise Citogenética
Neoplasias Hematológicas/diagnóstico
Seres Humanos
Imunofenotipagem
Hibridização in Situ Fluorescente
Células Progenitoras de Megacariócitos e Eritrócitos/metabolismo
Células Progenitoras de Megacariócitos e Eritrócitos/patologia
Megacariócitos/patologia
Indução de Remissão
Talidomida/administração & dosagem
Talidomida/efeitos adversos
Talidomida/uso terapêutico
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents); 4Z8R6ORS6L (Thalidomide); F0P408N6V4 (lenalidomide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180130
[St] Status:MEDLINE
[do] DOI:10.1111/bjh.15094


  7 / 5317 MEDLINE  
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[PMID]:29285580
[Au] Autor:Hidalgo López JE; Carballo-Zarate A; Verstovsek S; Wang SA; Hu S; Li S; Xu J; Zuo W; Tang Z; Yin CC; Medeiros LJ; Bueso-Ramos CE; Tang G
[Ad] Endereço:Department of Hematopathology, Unit 0072, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX, 77030, USA.
[Ti] Título:Bone marrow findings in blast phase of polycythemia vera.
[So] Source:Ann Hematol;97(3):425-434, 2018 Mar.
[Is] ISSN:1432-0584
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Approximately 10% of patients with polycythemia vera (PV) transform to acute leukemia (blast phase) at 10 years after initial diagnosis of PV. The bone marrow pathologic, cytogenetic, and molecular features of blast phase have not been well characterized. In this study, we reviewed 422 PV patients over a period of 11 years and identified 58 patients who developed acute myeloid leukemia (blast phase) during the course of disease. We found that blast phase of PV was characterized by overt myelodysplasia (n = 51, 88%); moderate to severe myelofibrosis (33 of 45, 73%); an abnormal karyotype (n = 51, 88%) that was often complex karyotype (n = 42, 72%); and gene mutations involving TP53 (55%), TET2 (27%), and DNMT3A (25%). Patients with blast phase of PV had an aggressive clinical course, with a median overall survival of 4 months after onset of blast phase. Eleven patients had close follow-up from polycythemic phase to blast phase: Four patients showed dysplastic changes in the polycythemic phase, and three of them transformed to blast phase without a "middle phase" of post-PV myelofibrosis.We conclude that blast phase of PV is characterized by myelodysplasia, moderate to severe fibrosis, a high frequency of an abnormal and often complex karyotype, and frequentTP53mutation.
[Mh] Termos MeSH primário: Crise Blástica/patologia
Medula Óssea/patologia
Leucemia Mieloide Aguda/patologia
Policitemia Vera/patologia
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Crise Blástica/genética
Medula Óssea/metabolismo
Transformação Celular Neoplásica/genética
Transformação Celular Neoplásica/patologia
Análise Citogenética
Progressão da Doença
Feminino
Seres Humanos
Leucemia Mieloide Aguda/genética
Masculino
Meia-Idade
Policitemia Vera/genética
Estudos Retrospectivos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171230
[St] Status:MEDLINE
[do] DOI:10.1007/s00277-017-3211-7


  8 / 5317 MEDLINE  
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[PMID]:29181548
[Au] Autor:Senín A; Fernández-Rodríguez C; Bellosillo B; Camacho L; Longarón R; Angona A; Besses C; Álvarez-Larrán A
[Ad] Endereço:Hematology Department, Hospital del Mar-IMIM (Institut Hospital del Mar d'Investigacions Mèdiques), Universidad Autónoma de Barcelona, Passeig Marítim 25-29, 08003, Barcelona, Spain.
[Ti] Título:Non-driver mutations in patients with JAK2V617F-mutated polycythemia vera or essential thrombocythemia with long-term molecular follow-up.
[So] Source:Ann Hematol;97(3):443-451, 2018 Mar.
[Is] ISSN:1432-0584
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:JAK2V617F monitoring and NGS of non-driver genes was performed in 100 patients with polycythemia vera (PV) or essential thrombocythemia (ET) with long molecular follow-up. Patients who did not progress to myelofibrosis (MF) or acute myeloid leukemia (AML) after more than 10 years (n = 50) showed a low frequency of mutations at first sample (18%) and an incidence rate of 1.7 new mutations × 100 person-years. Mutations were detected at first sample in 83% of PV/ET patients who later progressed to AML (n = 12) with these patients having a rate of 25.6 mutations × 100 person-years. Presence of mutations at diagnosis was the unique risk factor for acquiring a new genetic event (HR 2.7, 95% CI 1.1-6.8, p = 0.03) after correction for age, PV diagnosis, and total duration of hydroxyurea (HU) exposure. Patients with additional mutation at first sample showed a higher probability of developing cytopenia under HU therapy and a higher risk of AML (HR 12.2, 95% CI 2.6-57.1, p = 0.001) with mutations in ASXL1 (p < 0.0001), TP53 (p = 0.01), SRSF2 (p < 0.0001), IDH1/2 (p < 0.0001), and RUNX1 (p < 0.0001) being associated with a higher probability of AML. Myelofibrotic transformation was more frequent in patients with additional mutations, especially in SF3B1 (p = 0.02) and IDH1/2 (p < 0.0001) although a persistently high or a progressive increase of the JAK2V617F allele burden while receiving cytoreduction was the strongest predictor of MF transformation (HR 10.8, 95% CI 2.4-49.1, p = 0.002). In conclusion, NGS may be useful to identify a minority of PV and ET patients with high genetic instability and increased risk of AML transformation.
[Mh] Termos MeSH primário: Transformação Celular Neoplásica/genética
Janus Quinase 2/genética
Mutação de Sentido Incorreto
Policitemia Vera/genética
Trombocitemia Essencial/genética
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Alelos
Substituição de Aminoácidos
Análise Citogenética
Progressão da Doença
Feminino
Seguimentos
Frequência do Gene
Seres Humanos
Masculino
Meia-Idade
Fenilalanina/genética
Policitemia Vera/patologia
Trombocitemia Essencial/patologia
Valina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
47E5O17Y3R (Phenylalanine); EC 2.7.10.2 (JAK2 protein, human); EC 2.7.10.2 (Janus Kinase 2); HG18B9YRS7 (Valine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE
[do] DOI:10.1007/s00277-017-3193-5


  9 / 5317 MEDLINE  
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[PMID]:28456962
[Au] Autor:Ranisavljevic N; Okamoto I; Heard E; Ancelin K
[Ad] Endereço:Unité de Génétique et Biologie du Développement, Institut Curie, PSL Research University, CNRS UMR 3215, INSERM U934, 26 rue d'Ulm, 75248 Paris Cedex 05, France.
[Ti] Título:RNA FISH to Study Zygotic Genome Activation in Early Mouse Embryos.
[So] Source:Methods Mol Biol;1605:133-145, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Characterizing the maternal-to-zygotic transition (MZT) is a central question in embryogenesis, and is critical for our understanding of early post-fertilization events in mammals. High-throughput RNA sequencing (RNA Seq) of mouse oocytes and early embryos has recently revealed that elaborate transcription patterns of genes and repeats are established post-fertilization. This occurs in the context of the gradually depleted maternal pool of RNA provided by the oocyte, which can confound the accurate analysis of the zygotic genome activation when the mRNA population is sequenced. In this context, and given the limited amounts of material available from embryos, particularly when studying mutants, as well as the cost of sequencing, an alternative, complementary single cell approach is RNA FISH. This approach can assay the expression of specific genes or genetic elements during preimplantation development, in particular during the MZT. Here, we describe how RNA FISH can be applied to visualize nascent transcription at specific genomic loci in embryos at different stages of preimplantation development and also discuss possible analytical methods of RNA FISH data.
[Mh] Termos MeSH primário: Embrião de Mamíferos/química
Hibridização in Situ Fluorescente/métodos
RNA/genética
[Mh] Termos MeSH secundário: Animais
Análise Citogenética
Regulação da Expressão Gênica no Desenvolvimento
Camundongos
Análise de Célula Única
Ativação Transcricional
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
63231-63-0 (RNA)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170501
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-6988-3_9


  10 / 5317 MEDLINE  
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[PMID]:29246535
[Au] Autor:Taboada X; Rey M; Bouza C; Viñas A
[Ad] Endereço:Departamento de Zoología, Genética y Antropología Física, Facultad de Biología, CIBUS, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
[Ti] Título:Cytogenomic analysis of several repetitive DNA elements in turbot (Scophthalmus maximus).
[So] Source:Gene;644:4-12, 2018 Feb 20.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Repetitive DNA plays a fundamental role in the organization, size and evolution of eukaryotic genomes. The sequencing of the turbot revealed a small and compact genome, as in all flatfish studied to date. The assembly of repetitive regions is still incomplete because it is difficult to correctly identify their position, number and array. The combination of classical cytogenetic techniques along with high quality sequencing is essential to increase the knowledge of the structure and composition of these sequences and, thus, of the structure and function of the whole genome. In this work, the in silico analysis of H1 histone, 5S rDNA, telomeric and Rex repetitive sequences, was compared to their chromosomal mapping by fluorescent in situ hybridization (FISH), providing a more comprehensive picture of these elements in the turbot genome. FISH assays confirmed the location of H1 in LG8; 5S rDNA in LG4 and LG6; telomeric sequences at the end of all chromosomes whereas Rex elements were dispersed along most chromosomes. The discrepancies found between both approaches could be related to the sequencing methodology applied in this species and also to the resolution limitations of the FISH technique. Turbot cytogenomic analyses have proven to add new chromosomal landmarks in the karyotype of this species, representing a powerful tool to investigate targeted genomic sequences or regions in the genetic and physical maps of this species.
[Mh] Termos MeSH primário: DNA/genética
Linguados/genética
Sequências Repetitivas de Ácido Nucleico/genética
[Mh] Termos MeSH secundário: Animais
Mapeamento Cromossômico/métodos
Análise Citogenética/métodos
Genoma/genética
Hibridização in Situ Fluorescente/métodos
Cariótipo
RNA Ribossômico 5S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 5S); 9007-49-2 (DNA)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180119
[Lr] Data última revisão:
180119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171217
[St] Status:MEDLINE



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