Base de dados : MEDLINE
Pesquisa : E01.370.225.500.386 [Categoria DeCS]
Referências encontradas : 973 [refinar]
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[PMID]:29221729
[Au] Autor:Alcántara-Hernández M; Leylek R; Wagar LE; Engleman EG; Keler T; Marinkovich MP; Davis MM; Nolan GP; Idoyaga J
[Ad] Endereço:Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA; Program in Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
[Ti] Título:High-Dimensional Phenotypic Mapping of Human Dendritic Cells Reveals Interindividual Variation and Tissue Specialization.
[So] Source:Immunity;47(6):1037-1050.e6, 2017 Dec 19.
[Is] ISSN:1097-4180
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Given the limited efficacy of clinical approaches that rely on ex vivo generated dendritic cells (DCs), it is imperative to design strategies that harness specialized DC subsets in situ. This requires delineating the expression of surface markers by DC subsets among individuals and tissues. Here, we performed a multiparametric phenotypic characterization and unbiased analysis of human DC subsets in blood, tonsil, spleen, and skin. We uncovered previously unreported phenotypic heterogeneity of human cDC2s among individuals, including variable expression of functional receptors such as CD172a. We found marked differences in DC subsets localized in blood and lymphoid tissues versus skin, and a striking absence of the newly discovered Axl DCs in the skin. Finally, we evaluated the capacity of anti-receptor monoclonal antibodies to deliver vaccine components to skin DC subsets. These results offer a promising path for developing DC subset-specific immunotherapies that cannot be provided by transcriptomic analysis alone.
[Mh] Termos MeSH primário: Antígenos de Diferenciação/imunologia
Variação Biológica Individual
Células Dendríticas/imunologia
Fenótipo
Proteínas Proto-Oncogênicas/imunologia
Receptores Proteína Tirosina Quinases/imunologia
Receptores Imunológicos/imunologia
Pele/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Monoclonais/química
Anticorpos Monoclonais/metabolismo
Anticorpos Monoclonais/farmacocinética
Antígenos CD/genética
Antígenos CD/imunologia
Antígenos de Diferenciação/genética
Biomarcadores/análise
Vacinas Anticâncer/administração & dosagem
Vacinas Anticâncer/biossíntese
Citofotometria/métodos
Células Dendríticas/citologia
Feminino
Expressão Gênica
Seres Humanos
Imunofenotipagem
Imunoterapia
Linfonodos/citologia
Linfonodos/imunologia
Camundongos
Camundongos Endogâmicos C57BL
Terapia de Alvo Molecular
Neoplasias/genética
Neoplasias/imunologia
Neoplasias/patologia
Neoplasias/terapia
Especificidade de Órgãos
Tonsila Palatina/citologia
Tonsila Palatina/imunologia
Proteínas Proto-Oncogênicas/deficiência
Proteínas Proto-Oncogênicas/genética
Receptores Proteína Tirosina Quinases/deficiência
Receptores Proteína Tirosina Quinases/genética
Receptores Imunológicos/genética
Pele/citologia
Baço/citologia
Baço/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Antigens, CD); 0 (Antigens, Differentiation); 0 (Biomarkers); 0 (Cancer Vaccines); 0 (Proto-Oncogene Proteins); 0 (Receptors, Immunologic); 0 (SIRPA protein, human); EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases); EC 2.7.10.1 (axl receptor tyrosine kinase)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171210
[St] Status:MEDLINE


  2 / 973 MEDLINE  
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[PMID]:28257779
[Au] Autor:Bulychev AA; Komarova AV
[Ad] Endereço:Department of Biophysics, Faculty of Biology, Moscow State University, Moscow 119991, Russia. Electronic address: bulychev@biophys.msu.ru.
[Ti] Título:Photoregulation of photosystem II activity mediated by cytoplasmic streaming in Chara and its relation to pH bands.
[So] Source:Biochim Biophys Acta;1858(5):386-395, 2017 05.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Chloroplasts in vivo exposed to strong light export assimilates and excess reducing power to the cytoplasm for metabolic conversions and allocation to neighboring and distant organelles. The cytoplasmic streaming, being particularly fast in characean internodes, distributes the exported metabolites from brightly illuminated cell spots to light-limited regions, which is evident from the transient increase in chlorophyll fluorescence of shaded areas in response to illumination of distant cell regions situated upstream the liquid flow. It is not yet known whether long-distance communications between anchored chloroplasts are interfered by pH banding that commonly arises in characean internodes under the action of continuous or fluctuating light. In this study, microfluorometry, pH-microsensors, and local illumination were combined to examine long-distance transport and subsequent reentry of photosynthetic metabolites, including triose phosphates, into chloroplasts of cell regions producing external alkaline and acid bands. The lateral transmission of metabolic signals between distant chloroplasts was found to operate effectively in cell areas underlying acid zones but was almost fully blocked under alkaline zones. The rates of linear electron flow in chloroplasts of these regions were nearly equal under dim background light, but differed substantially at high light when availability of CO , rather than irradiance, was the rate-limiting factor. Different productions of assimilates by chloroplasts underlying CO -sufficient acid and CO -deficient alkaline zones were a cause for contrasting manifestations of long-distance transport of photosynthetic metabolites. Nonuniform cytoplasmic pH in cells exhibiting pH bands might contribute to different activities of metabolic translocators under high and low pH zones.
[Mh] Termos MeSH primário: Chara/efeitos da radiação
Cloroplastos/efeitos da radiação
Corrente Citoplasmática/efeitos da radiação
Transdução de Sinal Luminoso/efeitos da radiação
Luz
Fotossíntese/efeitos da radiação
Complexo de Proteína do Fotossistema II/efeitos da radiação
[Mh] Termos MeSH secundário: Chara/metabolismo
Cloroplastos/metabolismo
Citofotometria
Transferência de Energia
Concentração de Íons de Hidrogênio
Complexo de Proteína do Fotossistema II/metabolismo
Prótons
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Photosystem II Protein Complex); 0 (Protons)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170824
[Lr] Data última revisão:
170824
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170305
[St] Status:MEDLINE


  3 / 973 MEDLINE  
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[PMID]:27887728
[Au] Autor:Lyons JS; Iyer SR; Lovering RM; Ward CW; Stains JP
[Ad] Endereço:Department of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
[Ti] Título:Novel multi-functional fluid flow device for studying cellular mechanotransduction.
[So] Source:J Biomech;49(16):4173-4179, 2016 Dec 08.
[Is] ISSN:1873-2380
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cells respond to their mechanical environment by initiating multiple mechanotransduction signaling pathways. Defects in mechanotransduction have been implicated in a number of pathologies; thus, there is need for convenient and efficient methods for studying the mechanisms underlying these processes. A widely used and accepted technique for mechanically stimulating cells in culture is the introduction of fluid flow on cell monolayers. Here, we describe a novel, multifunctional fluid flow device for exposing cells to fluid flow in culture. This device integrates with common lab equipment including routine cell culture plates and peristaltic pumps. Further, it allows the fluid flow treated cells to be examined with outcomes at the cell and molecular level. We validated the device using the biologic response of cultured UMR-106 osteoblast-like cells in comparison to a commercially available system of laminar sheer stress to track live cell calcium influx in response to fluid flow. In addition, we demonstrate the fluid flow-dependent activation of phospho-ERK in these cells, consistent with the findings in other fluid flow devices. This device provides a low cost, multi-functional alternative to currently available systems, while still providing the ability to generate physiologically relevant conditions for studying processes involved in mechanotransduction in vitro.
[Mh] Termos MeSH primário: Citofotometria/instrumentação
Mecanotransdução Celular
[Mh] Termos MeSH secundário: Animais
Sinalização do Cálcio
Técnicas de Cultura de Células/instrumentação
Células Cultivadas
Hidrodinâmica
Osteoblastos/fisiologia
Ratos
Análise de Célula Única/instrumentação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161127
[St] Status:MEDLINE


  4 / 973 MEDLINE  
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[PMID]:27599517
[Au] Autor:Krutetskaya ZI; Milenina LS; Naumova AA; Antonov VG; Nozdrachev AD
[Ad] Endereço:St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia. z.krutetskaya@spbu.ru.
[Ti] Título:5-Lipoxygenase inhibitor zileuton inhibits Ca(2+)-responses induced by glutoxim and molixan in macrophages.
[So] Source:Dokl Biochem Biophys;469(1):302-4, 2016 Jul.
[Is] ISSN:1608-3091
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:Using Fura-2AM microfluorimetry, we have shown for the first time that 5-lipoxygenase specific inhibitor antiasthmatic agent zileuton significantly inhibits Ca(2+)-responses induced by glutoxim and molixan in macrophages. The results support 5-lipoxygenase involvement in the effect of glutoxim and molixan on intracellular Ca(2+) concentration in macrophages and indicate the inadvisability of a combined use of drugs glutoxim and molixan and antiasthmatic agent zileuton.
[Mh] Termos MeSH primário: Antiasmáticos/farmacologia
Hidroxiureia/análogos & derivados
Inosina/farmacologia
Inibidores de Lipoxigenase/farmacologia
Macrófagos Peritoneais/efeitos dos fármacos
Oligopeptídeos/farmacologia
[Mh] Termos MeSH secundário: Animais
Araquidonato 5-Lipoxigenase/metabolismo
Cálcio/metabolismo
Sinalização do Cálcio/efeitos dos fármacos
Sinalização do Cálcio/fisiologia
Cátions Bivalentes/metabolismo
Células Cultivadas
Citofotometria
Combinação de Medicamentos
Interações Medicamentosas
Fura-2/análogos & derivados
Hidroxiureia/farmacologia
Espaço Intracelular/efeitos dos fármacos
Espaço Intracelular/enzimologia
Macrófagos Peritoneais/enzimologia
Microscopia de Fluorescência
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Asthmatic Agents); 0 (Cations, Divalent); 0 (Drug Combinations); 0 (Lipoxygenase Inhibitors); 0 (Molixan); 0 (Oligopeptides); 0 (glutoxim); 105344-37-4 (fura-2-am); 5A614L51CT (Inosine); EC 1.13.11.34 (Arachidonate 5-Lipoxygenase); SY7Q814VUP (Calcium); TSN3DL106G (Fura-2); V1L22WVE2S (zileuton); X6Q56QN5QC (Hydroxyurea)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160908
[St] Status:MEDLINE
[do] DOI:10.1134/S1607672916040177


  5 / 973 MEDLINE  
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[PMID]:27561953
[Au] Autor:Liu B; Song S; Ruz-Maldonado I; Pingitore A; Huang GC; Baker D; Jones PM; Persaud SJ
[Ad] Endereço:Division of Diabetes and Nutritional Sciences, Diabetes Research Group, King's College London, London, UK.
[Ti] Título:GPR55-dependent stimulation of insulin secretion from isolated mouse and human islets of Langerhans.
[So] Source:Diabetes Obes Metab;18(12):1263-1273, 2016 Dec.
[Is] ISSN:1463-1326
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: The novel cannabinoid receptor GPR55 is expressed by rodent islets and it has been implicated in ß-cell function in response to a range of ligands. This study evaluated the effects of GPR55 ligands on intracellular calcium ([Ca ] ) levels and insulin secretion from islets isolated from GPR55 knockout (GPR55 ) mice, age-matched wildtype (WT) mice and human pancreas. MATERIALS AND METHODS: GPR55 expression was determined by Western blotting and fluorescent immunohistochemistry. Changes in [Ca ] were measured by Fura-2 microfluorimetry. Dynamic insulin secretion was quantified by radioimmunoassay following perifusion of isolated islets. RhoA activity was monitored using a Rho binding domain pull down assay. RESULTS: Western blotting indicated that MIN6 ß-cells, mouse and human islets express GPR55 and its localization on human ß-cells was demonstrated by fluorescent immunohistochemistry. The pharmacological GPR55 agonist O-1602 (10 µM) significantly stimulated [Ca ] and insulin secretion from WT mouse islets and these stimulatory effects were abolished in islets isolated from GPR55 mice. In contrast, while the putative endogenous GPR55 agonist lysophosphatidylinositol (LPI, 5 µM) and the GPR55 antagonist cannabidiol (CBD, 1 µM) also elevated [Ca ] and insulin secretion, these effects were sustained in islets from GPR55 mice. Stimulatory effects of O-1602 on [Ca ] and insulin secretion were also observed in experiments using human islets, but O-1602 did not activate RhoA in MIN6 ß-cells. CONCLUSIONS: Our results therefore suggest that GPR55 plays an important role in the regulation of mouse and human islet physiology, but LPI and CBD exert stimulatory effects on islet function by a GPR55-independent pathway(s).
[Mh] Termos MeSH primário: Canabidiol/farmacologia
Agonistas de Receptores de Canabinoides/farmacologia
Antagonistas de Receptores de Canabinoides/farmacologia
Cicloexanos/farmacologia
Células Secretoras de Insulina/efeitos dos fármacos
Lisofosfolipídeos/farmacologia
Receptores de Canabinoides/genética
Receptores Acoplados a Proteínas-G/metabolismo
Resorcinóis/farmacologia
[Mh] Termos MeSH secundário: Animais
Western Blotting
Cálcio/metabolismo
Linhagem Celular
Citofotometria
Eletroforese em Gel de Poliacrilamida
Seres Humanos
Imuno-Histoquímica
Insulina/secreção
Células Secretoras de Insulina/metabolismo
Células Secretoras de Insulina/secreção
Ilhotas Pancreáticas/efeitos dos fármacos
Ilhotas Pancreáticas/metabolismo
Ilhotas Pancreáticas/secreção
Camundongos
Camundongos Knockout
Imagem Óptica
Receptores de Canabinoides/metabolismo
Análise de Célula Única
Proteína rhoA de Ligação ao GTP/efeitos dos fármacos
Proteína rhoA de Ligação ao GTP/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cannabinoid Receptor Agonists); 0 (Cannabinoid Receptor Antagonists); 0 (Cyclohexanes); 0 (GPR55 protein, human); 0 (GPR55 protein, mouse); 0 (Insulin); 0 (Lysophospholipids); 0 (O 1602); 0 (Receptors, Cannabinoid); 0 (Receptors, G-Protein-Coupled); 0 (Resorcinols); 0 (lysophosphatidylinositol); 19GBJ60SN5 (Cannabidiol); EC 3.6.5.2 (rhoA GTP-Binding Protein); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160827
[St] Status:MEDLINE
[do] DOI:10.1111/dom.12780


  6 / 973 MEDLINE  
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[PMID]:27323800
[Au] Autor:Nassar AF; Wisnewski AV; Raddassi K
[Ad] Endereço:Department of Internal Medicine, School of Medicine, Yale University, New Haven, CT, USA.
[Ti] Título:Progress in automation of mass cytometry barcoding for drug development.
[So] Source:Bioanalysis;8(14):1429-35, 2016 Jul.
[Is] ISSN:1757-6199
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Citofotometria/métodos
Descoberta de Drogas/métodos
Ensaios de Triagem em Larga Escala/métodos
Espectrometria de Massas/métodos
[Mh] Termos MeSH secundário: Animais
Citofotometria/instrumentação
Descoberta de Drogas/instrumentação
Ensaios de Triagem em Larga Escala/instrumentação
Seres Humanos
Espectrometria de Massas/instrumentação
Paládio/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
5TWQ1V240M (Palladium)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170112
[Lr] Data última revisão:
170112
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160622
[St] Status:MEDLINE
[do] DOI:10.4155/bio-2016-0135


  7 / 973 MEDLINE  
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[PMID]:27055750
[Au] Autor:Ladyman MK; Walton JG; Lilienkampf A; Bradley M
[Ti] Título:Fluorescent Formazans and Tetrazolium Salts - Towards Fluorescent Cytotoxicity Assays.
[So] Source:Comb Chem High Throughput Screen;19(5):384-91, 2016.
[Is] ISSN:1875-5402
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Formazan-based colorimetric cytotoxicity assays, such as the MTT assay, are typically used to assess cell viability with only metabolically active cells reducing tetrazolium salts into the formazans, which is then quantified by absorbance. Fluorescence offers several advantages compared to colorimetric assays and would enable techniques such as flow cytometry and confocal microscopy to be used for analysis. Here, fluorescent formazans 10, 11 and 12, and their corresponding tetrazolium salts 13, 16 and 24, respectively, were synthesised by incorporation of a known fluorophore backbone (coumarin, fluorescein and rhodol) with disruption of the conjugated system preventing or reducing fluorescence of the tetrazolium salts. These tetrazolium salts were successfully reduced to the fluorescent formazans with cells and offer a step forward in the development of fluorescent cytotoxicity assays.
[Mh] Termos MeSH primário: Citofotometria/métodos
Formazans/química
Sais de Tetrazólio/química
[Mh] Termos MeSH secundário: Animais
Sobrevivência Celular
Células Cultivadas
Cumarínicos/química
Diagnóstico por Imagem/métodos
Fluoresceína/química
Fluorescência
Seres Humanos
Indicadores e Reagentes
Xantonas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coumarins); 0 (Formazans); 0 (Indicators and Reagents); 0 (Tetrazolium Salts); 0 (Xanthones); 0 (rhodol); A4VZ22K1WT (coumarin); TPY09G7XIR (Fluorescein)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170803
[Lr] Data última revisão:
170803
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160409
[St] Status:MEDLINE


  8 / 973 MEDLINE  
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[PMID]:27018769
[Au] Autor:Catena R; Özcan A; Zivanovic N; Bodenmiller B
[Ad] Endereço:Institute of Molecular Life Sciences (IMLS), University of Zürich, Zürich, Switzerland.
[Ti] Título:Enhanced multiplexing in mass cytometry using osmium and ruthenium tetroxide species.
[So] Source:Cytometry A;89(5):491-7, 2016 May.
[Is] ISSN:1552-4930
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mass cytometry facilitates high-dimensional, quantitative, single-cell analysis. The method for sample multiplexing in mass cytometry, called mass-tag cellular barcoding (MCB), relies on the covalent reaction of bifunctional metal chelators with intracellular proteins. Here, we describe the use of osmium and ruthenium tetroxides (OsO4 and RuO4 ) that bind covalently with fatty acids in the cellular membranes and aromatic amino acids in proteins. Both OsO4 and RuO4 rapidly reacted and allowed for MCB with live cells, crosslinked cells, and permeabilized cells. Given the covalent nature of the labeling reaction, isotope leaching was not observed. OsO4 and RuO4 were used in a 20-sample barcoding protocol together with palladium isotopes. As mass channels occupied by osmium and ruthenium are not used for antibody detection the number of masses effectively utilized in a single experiment is expanded. OsO4 and RuO4 can therefore be used as MCB reagents for a wide range of mass cytometry workflows. © 2016 International Society for Advancement of Cytometry.
[Mh] Termos MeSH primário: Citofotometria/métodos
Espectrometria de Massas/métodos
Tetróxido de Ósmio/química
Compostos de Rutênio/química
Análise de Célula Única/métodos
Coloração e Rotulagem/métodos
[Mh] Termos MeSH secundário: Aminoácidos/química
Anticorpos Monoclonais/química
Antígenos CD/análise
Linhagem Celular Tumoral
Quelantes/química
Citofotometria/instrumentação
Ácidos Graxos/química
Compostos Heterocíclicos com 1 Anel/química
Seres Humanos
Células Jurkat
Leucócitos Mononucleares/classificação
Leucócitos Mononucleares/citologia
Espectrometria de Massas/instrumentação
Paládio/química
Análise de Célula Única/instrumentação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Antibodies, Monoclonal); 0 (Antigens, CD); 0 (Chelating Agents); 0 (Fatty Acids); 0 (Heterocyclic Compounds, 1-Ring); 0 (Ruthenium Compounds); 1HTE449DGZ (1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid); 5TWQ1V240M (Palladium); 97E960G9RP (ruthenium tetraoxide); P40W033BGM (Osmium Tetroxide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160329
[St] Status:MEDLINE
[do] DOI:10.1002/cyto.a.22848


  9 / 973 MEDLINE  
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[PMID]:26736644
[Au] Autor:Otto O; Rosendahl P; Golfier S; Mietke A; Herbig M; Jacobi A; Topfner N; Herold C; Klaue D; Girardo S; Winzi M; Fischer-Friedrich E; Guck J
[Ti] Título:Real-time deformability cytometry as a label-free indicator of cell function.
[So] Source:Conf Proc IEEE Eng Med Biol Soc;2015:1861-4, 2015.
[Is] ISSN:1557-170X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The mechanical properties of cells are known to be a label-free, inherent marker of biological function in health and disease. Wide-spread utilization has so far been impeded by the lack of a convenient measurement technique with sufficient throughput. To address this unmet need, we have recently introduced real-time deformability cytometry (RT-DC) for continuous mechanical single-cell classification of heterogeneous cell populations at rates of several hundred cells per second. Cells are driven through the constriction zone of a microfluidic chip leading to cell deformations due to hydrodynamic stresses only. Our custom-built image processing software performs image acquisition, image analysis and data storage on the fly. The ensuing deformations can be quantified and an analytical model enables the derivation of cell material properties. Performing RT-DC we highlight its potential to identify rare objects in heterogeneous suspensions and to track drug-induced changes in cells. In summary, RT-DC enables marker-free, quantitative phenotyping of heterogeneous cell populations with a throughput comparable to standard flow cytometry.
[Mh] Termos MeSH primário: Citofotometria/instrumentação
Citofotometria/métodos
[Mh] Termos MeSH secundário: Desenho de Equipamento
Citometria de Fluxo/métodos
Células HL-60
Ensaios de Triagem em Larga Escala/instrumentação
Ensaios de Triagem em Larga Escala/métodos
Seres Humanos
Hidrodinâmica
Processamento de Imagem Assistida por Computador
Dispositivos Lab-On-A-Chip
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160107
[Lr] Data última revisão:
160107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160107
[St] Status:MEDLINE
[do] DOI:10.1109/EMBC.2015.7318744


  10 / 973 MEDLINE  
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[PMID]:26238588
[Au] Autor:Miyawaki A; Jaffrey SR
[Ad] Endereço:Laboratory of Cell Function Dynamics, Brain Science Institute, RIKEN, Wako, Saitama, Japan. Electronic address: matsushi@brain.riken.jp.
[Ti] Título:Editorial overview: Molecular imaging: Cellular imaging approaches.
[So] Source:Curr Opin Chem Biol;27:v-vi, 2015 Aug.
[Is] ISSN:1879-0402
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Pesquisa Biomédica/métodos
Citofotometria/métodos
Imagem Molecular/métodos
[Pt] Tipo de publicação:EDITORIAL
[Em] Mês de entrada:1605
[Cu] Atualização por classe:150828
[Lr] Data última revisão:
150828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150805
[St] Status:MEDLINE



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