Base de dados : MEDLINE
Pesquisa : E01.370.225.500.620 [Categoria DeCS]
Referências encontradas : 1308 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 131 ir para página                         

  1 / 1308 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28714056
[Au] Autor:Bragoni A; Gambella A; Pigozzi S; Grigolini M; Fiocca R; Mastracci L; Grillo F
[Ad] Endereço:Histopathology Unit, Department of Surgical Sciences and Integrated Diagnostics (DISC), University of Genoa, Genoa, Italy.
[Ti] Título:Quality control in diagnostic immunohistochemistry: integrated on-slide positive controls.
[So] Source:Histochem Cell Biol;148(5):569-573, 2017 Nov.
[Is] ISSN:1432-119X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Standardization in immunohistochemistry is a priority in modern pathology and requires strict quality control. Cost containment has also become fundamental and auditing of all procedures must take into account both these principles. Positive controls must be routinely performed so that their positivity guarantees the appropriateness of the immunohistochemical procedure. The aim of this study is to develop a low cost (utilizing a punch biopsy-PB-tool) procedure to construct positive controls which can be integrated in the patient's tissue slide. Sixteen frequently used control blocks were selected and multiple cylindrical samples were obtained using a 5-mm diameter punch biopsy tool, separately re-embedding them in single blocks. For each diagnostic immunoreaction requiring a positive control, an integrated PB-control section (cut from the appropriate PB-control block) was added to the top right corner of the diagnostic slide before immunostaining. This integrated control technique permitted a saving of 4.75% in total direct lab costs and proved to be technically feasible and reliable. Our proposal is easy to perform and within the reach of all pathology labs, requires easily available tools, its application costs is less than using external paired controls and ensures that a specific control for each slide is always available.
[Mh] Termos MeSH primário: Biópsia/normas
Técnicas de Preparação Histocitológica/normas
Imuno-Histoquímica/normas
Controle de Qualidade
[Mh] Termos MeSH secundário: Biópsia/economia
Biópsia/instrumentação
Técnicas de Preparação Histocitológica/economia
Técnicas de Preparação Histocitológica/instrumentação
Seres Humanos
Imuno-Histoquímica/economia
Imuno-Histoquímica/instrumentação
Padrões de Referência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170718
[St] Status:MEDLINE
[do] DOI:10.1007/s00418-017-1596-y


  2 / 1308 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28624120
[Au] Autor:Chung HJ; Goldberg LJ
[Ad] Endereço:Section of Dermatopathology, Department of Dermatology, Boston University School of Medicine, Boston, Massachusetts.
[Ti] Título:Histologic features of chronic cutaneous lupus erythematosus of the scalp using horizontal sectioning: Emphasis on follicular findings.
[So] Source:J Am Acad Dermatol;77(2):349-355, 2017 Aug.
[Is] ISSN:1097-6787
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Chronic cutaneous lupus erythematosus (CCLE) often affects the scalp resulting in scarring alopecia. While histopathologic findings of CCLE have been well described, there is little written on the morphologic changes to the hair follicles in this condition. OBJECTIVE: We aim to determine the histopathologic findings of hair follicles in CCLE of the scalp. METHODS: We conducted a retrospective study of 33 transversely sectioned skin biopsy specimens of CCLE of the scalp at the Skin Pathology Laboratory at Boston University between April 2005 and March 2015. RESULTS: New findings include basaloid follicular epithelium lacking hair follicles at deep levels, follicular miniaturization, increased catagen/telogen hair follicles, and pigment casts. Two histopathologic patterns could be discerned, an alopecia areatalike pattern and a lichen planopilarislike pattern. LIMITATIONS: Generalizability of a single-center experience may be limited. CONCLUSION: Follicular findings in CCLE of the scalp are reported. We hypothesize that the basaloid aggregates are remnants of hair follicles that are no longer actively cycling.
[Mh] Termos MeSH primário: Folículo Piloso/patologia
Lúpus Eritematoso Discoide/patologia
Dermatoses do Couro Cabeludo/patologia
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Doença Crônica
Feminino
Técnicas de Preparação Histocitológica/métodos
Seres Humanos
Masculino
Meia-Idade
Estudos Retrospectivos
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170619
[St] Status:MEDLINE


  3 / 1308 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28437474
[Au] Autor:Holtfreter MC; Neubauer H; Groten T; El-Adawy H; Pastuschek J; Richter J; Häussinger D; Pletz MW; Schleenvoigt BT
[Ad] Endereço:Tropical Medicine Unit, Department of Gastroenterology, Hepatology and Infectious Diseases, Faculty of Medicine, Heinrich-Heine-University, Düsseldorf, Germany.
[Ti] Título:Improvement of a tissue maceration technique for the determination of placental involvement in schistosomiasis.
[So] Source:PLoS Negl Trop Dis;11(4):e0005551, 2017 Apr.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Schistosomiasis in pregnancy may cause low birth weight, prematurity and stillbirth of the offspring. The placenta of pregnant women might be involved when schistosome ova are trapped in placental tissue. Standard histopathological methods only allow the examination of a limited amount of placental tissue and are therefore not sufficiently sensitive. Thus, placental schistosomiasis remains underdiagnosed and its role in contributing to schistosomiasis-associated pregnancy outcomes remains unclear. Here we investigated an advanced maceration method in order to recover a maximum number of schistosome ova from the placenta. We examined the effect of different potassium hydroxide (KOH) concentrations and different tissue fixatives with respect to maceration success and egg morphology. Placental tissue was kept either in 0.9% saline, 5% formalin or 70% ethanol and was macerated together with Schistosoma mansoni infested mouse livers and KOH 4% or 10%, respectively. We found that placenta maceration using 4% KOH at 37°C for 24 h was the most effective method: placental tissue was completely digested, egg morphology was well preserved and alkaline concentration was the lowest. Ethanol proved to be the best fixative for this method. Here we propose an improved maceration technique in terms of sensitivity, safety and required skills, which may enable its wider use also in endemic areas. This technique may contribute to clarifying the role of placental involvement in pregnant women with schistosomiasis.
[Mh] Termos MeSH primário: Técnicas de Preparação Histocitológica/métodos
Placenta/patologia
Placenta/parasitologia
Complicações Parasitárias na Gravidez/patologia
Schistosoma mansoni/isolamento & purificação
Esquistossomose/patologia
[Mh] Termos MeSH secundário: Animais
Feminino
Fixadores
Seres Humanos
Hidróxidos/química
Camundongos
Óvulo/parasitologia
Compostos de Potássio/química
Gravidez
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fixatives); 0 (Hydroxides); 0 (Potassium Compounds); WZH3C48M4T (potassium hydroxide)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170628
[Lr] Data última revisão:
170628
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170425
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005551


  4 / 1308 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28242761
[Au] Autor:Liang T; Dolai S; Xie L; Winter E; Orabi AI; Karimian N; Cosen-Binker LI; Huang YC; Thorn P; Cattral MS; Gaisano HY
[Ad] Endereço:From the Department of Medicine, University of Toronto, Toronto, Ontario M5S 1A8, Canada.
[Ti] Título: human pancreatic slice preparations offer a valuable model for studying pancreatic exocrine biology.
[So] Source:J Biol Chem;292(14):5957-5969, 2017 Apr 07.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A genuine understanding of human exocrine pancreas biology and pathobiology has been hampered by a lack of suitable preparations and reliance on rodent models employing dispersed acini preparations. We have developed an organotypic slice preparation of the normal portions of human pancreas obtained from cancer resections. The preparation was assessed for physiologic and pathologic responses to the cholinergic agonist carbachol (Cch) and cholecystokinin (CCK-8), including 1) amylase secretion, 2) exocytosis, 3) intracellular Ca responses, 4) cytoplasmic autophagic vacuole formation, and 5) protease activation. Cch and CCK-8 both dose-dependently stimulated secretory responses from human pancreas slices similar to those previously observed in dispersed rodent acini. Confocal microscopy imaging showed that these responses were accounted for by efficient apical exocytosis at physiologic doses of both agonists and by apical blockade and redirection of exocytosis to the basolateral plasma membrane at supramaximal doses. The secretory responses and exocytotic events evoked by CCK-8 were mediated by CCK-A and not CCK-B receptors. Physiologic agonist doses evoked oscillatory Ca increases across the acini. Supraphysiologic doses induced formation of cytoplasmic autophagic vacuoles and activation of proteases (trypsin, chymotrypsin). Maximal atropine pretreatment that completely blocked all the Cch-evoked responses did not affect any of the CCK-8-evoked responses, indicating that rather than acting on the nerves within the pancreas slice, CCK cellular actions directly affected human acinar cells. Human pancreas slices represent excellent preparations to examine pancreatic cell biology and pathobiology and could help screen for potential treatments for human pancreatitis.
[Mh] Termos MeSH primário: Exocitose
Técnicas de Preparação Histocitológica/métodos
Modelos Biológicos
Pâncreas Exócrino/metabolismo
Pancreatite/metabolismo
[Mh] Termos MeSH secundário: Adulto
Idoso
Feminino
Seres Humanos
Masculino
Meia-Idade
Pâncreas Exócrino/patologia
Pancreatite/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170601
[Lr] Data última revisão:
170601
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170301
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.777433


  5 / 1308 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28238098
[Au] Autor:Teng X; Zhang S; Liu W; Bi K; Zhang L
[Ad] Endereço:Department of Pathology, Beijing Friendship Hospital, Capital Medical University (Lymphoma Diagnostic Center, Beijing Institute of Clinical Medicine), Beijing, 100050, People's Republic of China. xiaojingteng@sina.com.
[Ti] Título:A new method for real-time evaluation of pepsin digestion of paraffin-embedded tissue sections, prior to fluorescence in situ hybridisation.
[So] Source:Virchows Arch;470(5):567-573, 2017 May.
[Is] ISSN:1432-2307
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Fluorescence in situ hybridisation (FISH) is a molecular cytogenetic technique, which is regularly applied to formalin-fixed paraffin-embedded (FFPE) tissue sections of a variety of cancers to assess chromosomal aberrations. However, high-quality FISH requires optimal enzymatic digestion, and insufficient digestion is not noted until the hybridisation signals are evaluated in the fluorescence microscope. As a consequence, FISH results may be unreliable, and the experiment might have to be repeated. To solve this problem, we developed a new method for real-time evaluation of enzymatic tissue digestion. Termination of enzyme activity at the proper time facilitates successful hybridisation, and experiments do not have to be repeated. We first performed FISH on 20 FFPE samples, which had been pepsin digested for different times, and this revealed distinct morphological changes within the nucleus and perinuclear space that were detectable by light microscopy. These observations suggested that the presence of intact and clear bare nuclei, surrounded by a translucent perinuclear space, might serve as an indicator of adequate digestion. We developed a protocol for assessment of this indicator, based on morphological features, and applied this to a collection of 400 tissue samples, partly of breast cancer and partly of different types of lymphoma, prior to FISH. The FISH success rate was 99.5% (398/400), which was significantly higher than that of the conventional method. In all successful cases, morphological signs of adequate digestion were paralleled by easily interpretable FISH signals. This new method for the real-time assessment of digestion quality improved the success rate of FISH and in addition was simple and rapid.
[Mh] Termos MeSH primário: Técnicas de Preparação Histocitológica
Hibridização in Situ Fluorescente/métodos
Pepsina A
[Mh] Termos MeSH secundário: Seres Humanos
Neoplasias/diagnóstico
Neoplasias/genética
Inclusão em Parafina
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.4.23.1 (Pepsin A)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170227
[St] Status:MEDLINE
[do] DOI:10.1007/s00428-017-2097-z


  6 / 1308 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28195650
[Au] Autor:Marcos R; Santos M; Marrinhas C; Caniatti M
[Ad] Endereço:Cytology Diagnostic Services, Institute of Biomedical Sciences Abel Salazar, University of Porto, ICBAS - UP, Porto, Portugal.
[Ti] Título:Cell tube block: a new technique to produce cell blocks from fluid cytology samples.
[So] Source:Vet Clin Pathol;46(1):195-201, 2017 Mar.
[Is] ISSN:1939-165X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Cell blocks are widely used in human cytopathology. Several techniques have been proposed to convert fluid specimens into solid material, which after embedding in paraffin can be used for histochemistry, immunohistochemistry, or molecular testing. In contrast, only in the last few years, have cell blocks begun to be used in the veterinary field. OBJECTIVES: The purpose of the study was to present the production and features of cell tube blocks (CTB) with veterinary liquid samples. METHODS: Liquid samples including cerebrospinal fluid, cutaneous cyst fluid, pericardial and pleural effusions, bronchoalveolar lavage, urine, and blood were centrifuged in a microhematocrit centrifuge. Capillary tubes were broken at the liquid-solid interface and fixed in 10% formalin for 24 hours. After paraffin embedding, sections of CTB were used for different stains including immunohistochemistry. RESULTS: The morphology and cellular detail in CTB sections were comparable to conventional histologic sections and other existing cell block techniques. The use of special stains such as Gram, Giemsa, alcian blue, and periodic acid-Schiff was straightforward, and immunohistochemistry results with antibodies to pan-cytokeratin, PAX-5, and CD3 were considered good. CONCLUSIONS: The CTB method was easily implementable under practice conditions (up to the fixation of the microhemtocrit tube), analogous to surgical biopsy submission for histology. Cell tube blocks can increase diagnostic accuracy when the technique is used in tandem after the cytologic evaluation, and the technique allows storage of fluids. Other advantages of CTB were the simplicity, low cost, and separation of erythrocytes from the nucleated cells, which was helpful in hemodiluted samples.
[Mh] Termos MeSH primário: Líquidos Corporais/citologia
Doenças do Gato/diagnóstico
Doenças do Cão/diagnóstico
Técnicas de Preparação Histocitológica/veterinária
[Mh] Termos MeSH secundário: Animais
Doenças do Gato/patologia
Gatos
Doenças do Cão/patologia
Cães
Histocitoquímica/métodos
Histocitoquímica/veterinária
Técnicas de Preparação Histocitológica/métodos
Imuno-Histoquímica/métodos
Imuno-Histoquímica/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170215
[St] Status:MEDLINE
[do] DOI:10.1111/vcp.12446


  7 / 1308 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28140513
[Au] Autor:La Fortune KA; Randolph ML; Wu HH; Cramer HM
[Ad] Endereço:Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, Indiana.
[Ti] Título:Improvements in cell block processing: The Cell-Gel method.
[So] Source:Cancer;125(4):267-276, 2017 Apr.
[Is] ISSN:1097-0142
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The ability to produce adequate cell blocks profoundly impacts the diagnostic usefulness of cytology specimens. Cell blocks are routinely processed from fine-needle aspiration specimens or concentrated fluid samples. Obtaining directed passes for the sole purpose of producing a cell block is common practice, particularly when the cytopathologist anticipates the need for ancillary immunocytochemical stains and/or molecular studies. METHODS: The authors developed an effective and inexpensive process for producing cell blocks that consistently yields abundant cellular material, which they have termed the Cell-Gel method. This method can be simplified into 3 main steps: 1) preparing the sample; 2) constructing the cell block; and 3) processing the cell block. Highlights of the protocol include using a hemolytic fixative for sample preparation and disposable base molds for cell block construction. RESULTS: The cell block failure rate in the current study decreased from 18% with the HistoGel Tube method (January 2014-December 2014) to 6% with the Cell-Gel method (January 2015-December 2016). The authors evaluated 110 cell blocks processed with the HistoGel Tube method and 110 cell blocks processed with the Cell-Gel method, for a total evaluation of 220 cell blocks. CONCLUSIONS: The authors have developed an effective and inexpensive protocol for producing cell blocks that consistently yields abundant cellular material. The Cell-Gel method uses a hemolytic fixative and disposable base molds to produce adequate cell blocks. When the method was implemented, the cell block failure rate of the study laboratory decreased by approximately 67%. Cancer Cytopathol 2017;125:267-276. © 2016 American Cancer Society.
[Mh] Termos MeSH primário: Citodiagnóstico/métodos
Técnicas de Preparação Histocitológica/métodos
Neoplasias/patologia
[Mh] Termos MeSH secundário: Citodiagnóstico/economia
Citodiagnóstico/instrumentação
Géis
Técnicas de Preparação Histocitológica/economia
Técnicas de Preparação Histocitológica/instrumentação
Seres Humanos
Imuno-Histoquímica/métodos
Neoplasias/diagnóstico
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gels)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170420
[Lr] Data última revisão:
170420
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170201
[St] Status:MEDLINE
[do] DOI:10.1002/cncy.21814


  8 / 1308 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27893537
[Au] Autor:Godsey T; Jacobson R; Gloster H
[Ad] Endereço:Department of Dermatology, University of Cincinnati, Cincinnati, Ohio.
[Ti] Título:A Novel Method of Processing Single Sections Too Large to Fit on One Glass Slide in Mohs Micrographic Surgery.
[So] Source:Dermatol Surg;43(7):987-989, 2017 07.
[Is] ISSN:1524-4725
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Técnicas de Preparação Histocitológica
Cirurgia de Mohs
Neoplasias Cutâneas/patologia
Neoplasias Cutâneas/cirurgia
[Mh] Termos MeSH secundário: Secções Congeladas
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161129
[St] Status:MEDLINE
[do] DOI:10.1097/DSS.0000000000000994


  9 / 1308 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27826759
[Au] Autor:Kipp M; Kiessling MC; Hochstrasser T; Roggenkamp C; Schmitz C
[Ad] Endereço:Department of Neuroanatomy, Ludwig-Maximilians University of Munich, 11 Pettenkoferstr, 80336, Munich, Germany.
[Ti] Título:Design-Based Stereology for Evaluation of Histological Parameters.
[So] Source:J Mol Neurosci;61(3):325-342, 2017 Mar.
[Is] ISSN:1559-1166
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Valid quantification of organ volume and total cell numbers are crucial parameters for morphometric studies. The number of a specific cell type cannot be simply deduced from the number of its profiles found in thin tissue sections, as this parameter also depends on cell volume, tissue orientation as well as tissue atrophy. Design-based stereology has become the method of choice for unbiased, reproducible total cell number quantification. Steps described in this protocol include transcardial perfusion of mice, postfixation, and cryoprotection of the region of interest (ROI), followed by the preparation of a systematically and randomly sampled series of thick sections through the entire ROI. Furthermore, it is described how to perform immuno-histochemical staining of such thick cryo-sections, followed by providing a guidance for quantification of the ROI volume, the generation of unbiased virtual counting spaces, and steps to work with these counting spaces to obtain an unbiased estimate of total cell numbers.
[Mh] Termos MeSH primário: Técnicas de Preparação Histocitológica/métodos
Imagem Óptica/métodos
[Mh] Termos MeSH secundário: Animais
Técnicas de Preparação Histocitológica/normas
Camundongos
Imagem Óptica/normas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161110
[St] Status:MEDLINE
[do] DOI:10.1007/s12031-016-0858-9


  10 / 1308 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27264533
[Au] Autor:Mansour AG; Khalil PA; Bejjani N; Chatila R; Dagher-Hamalian C; Faour WH
[Ad] Endereço:School of Medicine, Lebanese American University, Byblos, Lebanon.
[Ti] Título:An optimized xylene-free protein extraction method adapted to formalin-fixed paraffin embedded tissue sections for western blot analysis.
[So] Source:Histol Histopathol;32(3):307-313, 2017 Mar.
[Is] ISSN:1699-5848
[Cp] País de publicação:Spain
[La] Idioma:eng
[Ab] Resumo:Deparaffinization of formalin-fixed paraffin embedded (FFPE) tissues with xylene currently remains a major challenge to the biomedical community. We developed an efficient xylene-free protocol to isolate proteins from archived FFPE human tissue sections. A total of 79 different types of FFPE tissue sections of 8 µm thickness were obtained from various archived FFPE specimens. Deparaffinization was conducted by gently washing each section with around 1 ml of hot distilled water (≈80°C). The deparaffinized tissues were homogenized in lysis buffer, and the isolated proteins were quantified and efficiently resolved using western blot analysis for the presence of Protein kinase B (PKB/AKT) and ß-actin. Moreover, a significant amount of proteins was successfully isolated with an average of 2.31 µg/µl. The migration pattern of AKT and ß-actin obtained from the specimens was similar to the positive control obtained from protein lysates prepared from in vitro cultured MDA231 cancer cell lines. AKT was successfully identified in all specimens, and ß-actin protein was resolved with an efficiency higher than 80%. The entire extraction procedure requires only 20 minutes. This newly developed technique is an efficient, safe, cost-effective, and rapid method to isolate proteins from FFPE tissue sections adequate for molecular analysis.
[Mh] Termos MeSH primário: Biomarcadores Tumorais/análise
Western Blotting/métodos
Técnicas de Preparação Histocitológica
Proteínas/isolamento & purificação
[Mh] Termos MeSH secundário: Seres Humanos
Inclusão em Parafina
Fixação de Tecidos
Xilenos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (Proteins); 0 (Xylenes)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170420
[Lr] Data última revisão:
170420
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160607
[St] Status:MEDLINE
[do] DOI:10.14670/HH-11-789



página 1 de 131 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde