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[PMID]:29346450
[Au] Autor:Sridharan J; Haremaki T; Weinstein DC
[Ad] Endereço:Biology Department, Queens College of the City University of New York, Flushing, New York, United States of America.
[Ti] Título:Cloning and spatiotemporal expression of Xenopus laevis Apolipoprotein CI.
[So] Source:PLoS One;13(1):e0191470, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Apolipoprotein CI (ApoCI) belongs to the Apolipoprotein superfamily, members of which are involved in lipid transport, uptake and homeostasis. Excessive ApoCI has been implicated in atherosclerosis and Alzheimer's disease in humans. In this study we report the isolation of Xenopus laevis apoCI and describe the expression pattern of this gene during early development, using reverse transcription polymerase chain reaction and whole mount in situ hybridization. Xenopus apoCI is enriched in the dorsal ectoderm during gastrulation, and is subsequently expressed in sensory placodes, neural tube and cranial neural crest. These data suggest as yet uncharacterized roles for ApoCI during early vertebrate embryogenesis.
[Mh] Termos MeSH primário: Apolipoproteína C-I/genética
[Mh] Termos MeSH secundário: Animais
Clonagem Molecular
Gastrulação
Hibridização In Situ
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Xenopus laevis/embriologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Apolipoprotein C-I)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180119
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191470


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[PMID]:27773872
[Au] Autor:Willmore-Payne C; Damjanovich-Colmenares K; Pasi AV; Werner TL; Gulbahce HE; Downs-Kelly E; Geiersbach KB
[Ad] Endereço:From the ARUP Laboratories, Salt Lake City, UT.
[Ti] Título:Inconsistent Results With Different Secondary Reflex Assays for Resolving HER2 Status.
[So] Source:Am J Clin Pathol;146(5):618-626, 2016 Nov 01.
[Is] ISSN:1943-7722
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Objectives: Guidelines suggest that secondary reflex testing may be useful for resolving HER2 status in breast cancers with equivocal results by both immunohistochemistry (IHC) and in situ hybridization (ISH). We compared two reflex ISH assays and a polymerase chain reaction (PCR) assay for this application. Methods: Twenty-nine breast cancers with equivocal IHC and ISH results were retested two ways: (1) ISH using differentially labeled probes targeting ERBB2 ( HER2 , 17q12) and either RAI1 (17p11.2) or ORC4 (2q22.3-2q23.1) in two separate assays and (2) real-time quantitative PCR amplification of ERBB2 and a control locus ( EIF5B , 2q11.2). Results: Results of the HER2 / RAI1 and HER2 / ORC4 ISH assays were concordant for 21 (72%) cases, and results of all three secondary reflex assays were concordant for only 18 (62%) cases. Result discrepancies between the two ISH readers were observed for cases close to the cutoff threshold. Conclusions: Use of different control loci for ISH is associated with discordant results, and PCR is more likely to classify cases as nonamplified, possibly due to contamination with nontumor cells. While resolution of HER2 -equivocal results is desirable from a clinical perspective, different secondary reflex assays yield different results, and the correlation of these results with clinical outcomes is unknown.
[Mh] Termos MeSH primário: Biomarcadores Tumorais/metabolismo
Neoplasias da Mama/metabolismo
Receptor ErbB-2/metabolismo
[Mh] Termos MeSH secundário: Neoplasias da Mama/diagnóstico
Neoplasias da Mama/patologia
Proteínas de Ciclo Celular/genética
Proteínas de Ciclo Celular/metabolismo
Feminino
Seres Humanos
Imuno-Histoquímica
Hibridização In Situ
Complexo de Reconhecimento de Origem/genética
Complexo de Reconhecimento de Origem/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Receptor ErbB-2/genética
Reflexo
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (Cell Cycle Proteins); 0 (ORC4 protein, human); 0 (Origin Recognition Complex); 0 (RAI1 protein, human); 0 (Transcription Factors); EC 2.7.10.1 (ERBB2 protein, human); EC 2.7.10.1 (Receptor, ErbB-2)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1093/ajcp/aqw177


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[PMID]:29429163
[Au] Autor:Wu YL; Wu F; Yang L; Sun H; Yan XC; Duan GJ
[Ad] Endereço:Department of Pathology, the First Affiliated Hospital, Army Medical University (Third Military Medical University), PLA, Chongqing 400038, China.
[Ti] Título:[Clinicopathologic features and prognosis of inflammatory pseudotumor-like follicular dendritic cell sarcomas in liver and spleen: an analysis of seven cases].
[So] Source:Zhonghua Bing Li Xue Za Zhi;47(2):114-118, 2018 Feb 08.
[Is] ISSN:0529-5807
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To investigate the clinicopathological features and prognostic parameters of the inflammatory pseudotumor-like follicular dendritic cell sarcoma (IPT-like FDCS) of liver and spleen. Ninteen cases of inflammatory pseudotumor (IPT) and 5 cases of IPT-like FDCS of the liver and spleen were collected at the First Affiliated Hospital, Army Medical University from 2006 to 2016. HE sections, immunohistochemical staining, and Epstein-Barr virus encoded nuclear RNA (EBER) in situ hybridization were reviewed along with a summary of the literature. Among the previously diagnosed 19 cases of IPT of the liver and spleen, 2 cases were misdiagnosed (the ratio of 2/19). Among 7 new cases including 3 males and 4 females, 3 cases involved the liver and 4 cases involved the spleen. The age range was 37-64 years (mean 53 years). The maximum tumor diameter ranged from 3.0 to 11.0 cm (mean 6.5 cm). Surgical resections were performed in all patients with follow-up time ranging from 3 to 84 months.All patients were disease-free.7 new cases were all positive for EBER, and showed the expression of at least one of the FDC markers, including CD21, CD23, and CD35. The rest of 17 cases of IPT were all negative for EBER and essentially negative for FDC markers, but were all positive for SMA. IPT-like FDCS of the liver and spleen is a rare low-grade malignant tumor morphologically mimicking inflammatory pseudotumor, and is easy to be misdiagnosis due to under-recognition. EBER in situ hybridization and FDC markers are indispensable for confirming the diagnosis.
[Mh] Termos MeSH primário: Granuloma de Células Plasmáticas/patologia
Hepatopatias/patologia
Esplenopatias/patologia
[Mh] Termos MeSH secundário: Adulto
Sarcoma de Células Dendríticas Foliculares/patologia
Feminino
Herpesvirus Humano 4/genética
Seres Humanos
Hibridização In Situ
Masculino
Meia-Idade
Prognóstico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180213
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.0529-5807.2018.02.007


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[PMID]:28448987
[Au] Autor:Weitekamp CA; Solomon-Lane TK; Del Valle P; Triki Z; Nugent BM; Hofmann HA
[Ad] Endereço:Department of Integrative Biology, The University of Texas at Austin, Austin, TX, USA.
[Ti] Título:A Role for Oxytocin-Like Receptor in Social Habituation in a Teleost.
[So] Source:Brain Behav Evol;89(3):153-161, 2017.
[Is] ISSN:1421-9743
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Oxytocin (OT) mediates social habituation in rodent model systems, but its role in mediating this effect in other vertebrates is unknown. We used males of the African cichlid fish, Astatotilapia burtoni, to investigate two aspects of isotocin (IT; an OT homolog) signaling in social habituation. First, we examined the expression of IT receptor 2 (ITR2) as well as two immediate early genes in brain regions implicated in social recognition. Next, we examined IT neuron activity using immunohistochemistry. Patterns of gene expression in homologs of the amygdala and hippocampus implicate IT signaling in these regions in social habituation to a territorial neighbor. In the preoptic area, the expression of the ITR2 subtype and IT neuron activity respond to the presence of a male, independent of familiarity. Our results implicate IT in mediating social habituation in a teleost.
[Mh] Termos MeSH primário: Ciclídeos/genética
Ocitocina/análogos & derivados
[Mh] Termos MeSH secundário: Agressão/fisiologia
Tonsila do Cerebelo
Animais
Comportamento Animal/fisiologia
Encéfalo/metabolismo
Ciclídeos/fisiologia
Feminino
Hibridização In Situ
Masculino
Ocitocina/metabolismo
Ocitocina/fisiologia
Área Pré-Óptica/metabolismo
Receptores de Ocitocina/fisiologia
Comportamento Social
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, Oxytocin); 50-56-6 (Oxytocin); 550-21-0 (isotocin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE
[do] DOI:10.1159/000464098


  5 / 49096 MEDLINE  
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[PMID]:28456077
[Au] Autor:Zhao Y; Wang Y; Gong J; Yang L; Niu C; Ni X; Wang Y; Peng S; Gu X; Sun C; Yang Y
[Ad] Endereço:Key Laboratory of Neuroregeneration, Nantong University, Nantong 226007, PR China; Co-innovation Center of Neuroregeneration, Jiangsu Province, Nantong 226007, PR China.
[Ti] Título:Chitosan degradation products facilitate peripheral nerve regeneration by improving macrophage-constructed microenvironments.
[So] Source:Biomaterials;134:64-77, 2017 Jul.
[Is] ISSN:1878-5905
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Chitosan-based artificial nerve grafts have been widely employed to repair peripheral nerve defects. Our previous study has shown that chitosan constructed nerve graft not only provides suitable scaffolds for nerve regeneration, its degradation products, chitooligosaccharides (COS), also promote nerve repair. However, the involved mechanisms are still not fully elucidated. In the present study, we observed that pro-inflammatory cytokines, as well as macrophage infiltration, were transiently up-regulated in the injured sciatic nerves which were bridged with silicon tubes filled with COS. Based upon transcriptome analysis, the axis of miR-327/CCL2 in Schwann cells (SCs) was identified as a potential target of COS. The following experiments have confirmed that COS stimulate CCL2 expression by down-regulating miR-327 in SCs. Consequently, the resulting CCL2 induces macrophage migration at injury sites to re-construct microenvironments and thus facilitates nerve regeneration. Collectively, our data provide a theoretical basis for the clinical application of chitosan-based grafts in peripheral nerve regeneration.
[Mh] Termos MeSH primário: Quitosana/química
Quitosana/farmacologia
Macrófagos/efeitos dos fármacos
Macrófagos/metabolismo
Regeneração Nervosa/efeitos dos fármacos
Nervos Periféricos/citologia
Nervos Periféricos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Proliferação Celular/efeitos dos fármacos
Quitina/análogos & derivados
Quitina/química
Quitina/farmacologia
Cromatografia Líquida de Alta Pressão
Biologia Computacional
Ensaio de Imunoadsorção Enzimática
Células HEK293
Seres Humanos
Imuno-Histoquímica
Hibridização In Situ
Masculino
Camundongos
Células RAW 264.7
Ratos
Ratos Sprague-Dawley
Reação em Cadeia da Polimerase em Tempo Real
Células de Schwann/efeitos dos fármacos
Células de Schwann/metabolismo
Cicatrização/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (oligochitosan); 1398-61-4 (Chitin); 9012-76-4 (Chitosan)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


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[PMID]:29366791
[Au] Autor:Ganareal TACS; Balbin MM; Monserate JJ; Salazar JR; Mingala CN
[Ad] Endereço:Department of Chemistry, College of Arts and Sciences, Central Luzon State University, Science City of Muñoz 3120, Nueva Ecija, Philippines.
[Ti] Título:Gold nanoparticle-based probes for the colorimetric detection of Mycobacterium avium subspecies paratuberculosis DNA.
[So] Source:Biochem Biophys Res Commun;496(3):988-997, 2018 02 12.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Gold nanoparticle (AuNP) is considered to be the most stable metal nanoparticle having the ability to be functionalized with biomolecules. Recently, AuNP-based DNA detection methods captured the interest of researchers worldwide. Paratuberculosis or Johne's disease, a chronic gastroenteritis in ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP), was found to have negative effect in the livestock industry. In this study, AuNP-based probes were evaluated for the specific and sensitive detection of MAP DNA. AuNP-based probe was produced by functionalization of AuNPs with thiol-modified oligonucleotide and was confirmed by Fourier-Transform Infrared (FTIR) spectroscopy. UV-Vis spectroscopy and Scanning Electron Microscopy (SEM) were used to characterize AuNPs. DNA detection was done by hybridization of 10 µL of DNA with 5 µL of probe at 63 °C for 10 min and addition of 3 µL salt solution. The method was specific to MAP with detection limit of 103 ng. UV-Vis and SEM showed dispersion and aggregation of the AuNPs for the positive and negative results, respectively, with no observed particle growth. This study therefore reports an AuNP-based probes which can be used for the specific and sensitive detection of MAP DNA.
[Mh] Termos MeSH primário: Colorimetria/métodos
DNA/genética
DNA/isolamento & purificação
Ouro/química
Nanopartículas Metálicas/química
Mycobacterium avium/genética
Mycobacterium avium/isolamento & purificação
[Mh] Termos MeSH secundário: Técnicas Biossensoriais/métodos
Hibridização In Situ/métodos
Técnicas de Sonda Molecular
Sondas Moleculares/química
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Molecular Probes); 7440-57-5 (Gold); 9007-49-2 (DNA)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE


  7 / 49096 MEDLINE  
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[PMID]:29293602
[Au] Autor:Qian J; Mummalaneni S; Grider JR; Damaj MI; Lyall V
[Ad] Endereço:Physiology and Biophysics Virginia Commonwealth University, Richmond, VA, United States of America.
[Ti] Título:Nicotinic acetylcholine receptors (nAChRs) are expressed in Trpm5 positive taste receptor cells (TRCs).
[So] Source:PLoS One;13(1):e0190465, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Nicotine evokes chorda tympani (CT) taste nerve responses and an aversive behavior in Trpm5 knockout (KO) mice. The agonists and antagonists of nicotinic acetylcholine receptors (nAChRs) modulate neural and behavioral responses to nicotine in wildtype (WT) mice, Trpm5 KO mice and rats. This indicates that nicotine evokes bitter taste by activating a Trpm5-dependent pathway and a Trpm5-independent but nAChR-dependent pathway. Rat CT responses to ethanol are also partially inhibited by nAChR blockers, mecamylamine and dihydro-ß-erythroidine. This indicates that a component of the bitter taste of ethanol is also nAChR-dependent. However, at present the expression and localization of nAChR subunits has not been investigated in detail in taste receptor cells (TRCs). To this end, in situ hybridization, immunohistochemistry and q-RT-PCR techniques were utilized to localize nAChR subunits in fungiform and circumvallate TRCs in WT mice, Trpm5-GFP transgenic mice, nAChR KO mice, and rats. The expression of mRNAs for α7, ß2 and ß4 nAChR subunits was observed in a subset of rat and WT mouse circumvallate and fungiform TRCs. Specific α3, α4, α7, ß2, and ß4 antibodies localized to a subset of WT mouse circumvallate and fungiform TRCs. In Trpm5-GFP mice α3, α4, α7, and ß4 antibody binding was observed in a subset of Trpm5-positive circumvallate TRCs. Giving nicotine (100 µg/ml) in drinking water to WT mice for 3 weeks differentially increased the expression of α3, α4, α5, α6, α7, ß2 and ß4 mRNAs in circumvallate TRCs to varying degrees. Giving ethanol (5%) in drinking water to WT mice induced an increase in the expression of α5 and ß4 mRNAs in circumvallate TRCs with a significant decrease in the expression of α3, α6 and ß2 mRNAs. We conclude that nAChR subunits are expressed in Trpm5-positive TRCs and their expression levels are differentially altered by chronic oral exposure to nicotine and ethanol.
[Mh] Termos MeSH primário: Receptores Nicotínicos/metabolismo
Canais de Cátion TRPM/metabolismo
Papilas Gustativas/fisiologia
[Mh] Termos MeSH secundário: Animais
Hibridização In Situ
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Transgênicos
Ratos
Ratos Sprague-Dawley
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Receptors, Nicotinic); 0 (TRPM Cation Channels); 0 (TRPM5 protein, rat); 0 (Trpm5 protein, mouse)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190465


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[PMID]:29355689
[Au] Autor:Gryshkova V; Fleming A; McGhan P; De Ron P; Fleurance R; Valentin JP; Nogueira da Costa A
[Ad] Endereço:Investigative Toxicology, Non-Clinical Development, UCB Biopharma SPRL, Belgium.
[Ti] Título:miR-21-5p as a potential biomarker of inflammatory infiltration in the heart upon acute drug-induced cardiac injury in rats.
[So] Source:Toxicol Lett;286:31-38, 2018 Apr.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Investigation of genomic changes in cardiotoxicity can provide novel biomarkers and insights into molecular mechanisms of drug-induced cardiac injury (DICI). The main objective of this study was to identify and characterize dysregulated microRNAs (miRNAs) in the heart associated with cardiotoxicity. Wistar rats were dosed once with either isoproterenol (1.5 mg/kg, i.p), allylamine (100 mg/kg, p.o.) or the respective vehicle controls. Heart tissue was collected at 24 h, 48 h and 72 h post-drug administration and used for histopathological assessment, miRNA profiling, immunohistochemical analysis and in situ hybridization. Multiplex analysis of 68 miRNAs in the heart revealed a significant upregulation of several miRNAs (miR-19a-3p, miR-142-3p, miR-155-5p, miR-208b-3p, miR-21-5p) after isoproterenol and one miRNA (miR-21-5p) after allylamine administration. Localization of miR-21-5p was specific to inflammatory cell infiltrates in the heart after both treatments. Immunohistochemical analysis of Stat3, a known miR-21-5p regulator, also confirmed its upregulation in cardiomyocytes and inflammatory cell infiltrates. The toxicity signatures based on miRNA networks, identified in vivo, can potentially be used as mechanistic biomarkers as well as to study cardiotoxicity in vitro in order to develop sensitive tools for early hazard identification and risk assessment.
[Mh] Termos MeSH primário: Cardiopatias/induzido quimicamente
Inflamação/induzido quimicamente
MicroRNAs/genética
Miocárdio/metabolismo
[Mh] Termos MeSH secundário: Alilamina
Animais
Cardiotoxicidade
Modelos Animais de Doenças
Perfilação da Expressão Gênica/métodos
Regulação da Expressão Gênica
Marcadores Genéticos
Cardiopatias/genética
Cardiopatias/metabolismo
Cardiopatias/patologia
Hibridização In Situ
Inflamação/genética
Inflamação/metabolismo
Inflamação/patologia
Isoproterenol
Masculino
MicroRNAs/metabolismo
Reação em Cadeia da Polimerase Multiplex
Miocárdio/patologia
Análise de Sequência com Séries de Oligonucleotídeos
Ratos Wistar
Medição de Risco
Fator de Transcrição STAT3/metabolismo
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers); 0 (MicroRNAs); 0 (STAT3 Transcription Factor); 0 (Stat3 protein, rat); 0 (mirn21 microRNA, rat); 48G762T011 (Allylamine); L628TT009W (Isoproterenol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180123
[St] Status:MEDLINE


  9 / 49096 MEDLINE  
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[PMID]:27770977
[Au] Autor:Gray SG; Cuffe S; Finn SP
[Ad] Endereço:Labmed Directorate, St. James's Hospital, Dublin, Ireland. Electronic address: sgray@stjames.ie.
[Ti] Título:PD-L1 as a Companion Biomarker for Immune Checkpoint Inhibitors in NSCLC: Should RNA ISH (RISH) Be Considered?
[So] Source:J Thorac Oncol;11(11):e142-e144, 2016 11.
[Is] ISSN:1556-1380
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Antígeno B7-H1/genética
Biomarcadores/análise
Carcinoma Pulmonar de Células não Pequenas/diagnóstico
Pontos de Checagem do Ciclo Celular/imunologia
Hibridização In Situ/métodos
Neoplasias Pulmonares/diagnóstico
RNA Mensageiro/genética
[Mh] Termos MeSH secundário: Antineoplásicos/uso terapêutico
Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico
Carcinoma Pulmonar de Células não Pequenas/genética
Carcinoma Pulmonar de Células não Pequenas/imunologia
Pontos de Checagem do Ciclo Celular/efeitos dos fármacos
Seres Humanos
Neoplasias Pulmonares/tratamento farmacológico
Neoplasias Pulmonares/genética
Neoplasias Pulmonares/imunologia
[Pt] Tipo de publicação:LETTER
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (B7-H1 Antigen); 0 (Biomarkers); 0 (CD274 protein, human); 0 (RNA, Messenger)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


  10 / 49096 MEDLINE  
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[PMID]:29227650
[Au] Autor:Wang X; Guo L; Gao L; Shi X; Zhao X; Ma X; Xia T; Wang Y
[Ad] Endereço:Life Science College and §State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University , Hefei, Anhui 230036, People's Republic of China.
[Ti] Título:Molecular Evidence for Catechin Synthesis and Accumulation in Tea Buds (Camellia sinensis).
[So] Source:J Agric Food Chem;66(1):63-69, 2018 Jan 10.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Early spring buds of the Camellia sinensis variety Shuchazao were separated into two parts, including the shoot tip (ST) and non-expanded young leaves (YL), in which the synthesis and accumulation of catechins in the two parts were assessed by high-performance liquid chromatography (HPLC), p-dimethylaminocinnamaldehyde (DMACA) staining, quantitative real-time polymerase chain reaction (qRT-PCR), and in situ hybridization. HPLC showed that (-)-epigallocatechin-3-gallate (EGCG) and (-)-epicatechin-3-gallate (ECG) amounts in YL were increased significantly by 74.0 and 71.8%, respectively. The results of DMACA staining indicated that catechins in buds accumulated mainly in mesophyll cells and the bud shaft of YL. Meanwhile, qRT-PCR demonstrated that the relative expression levels of genes related to flavonoid metabolism, including CsPAL1, CsC4H1, CsC4H2, CsCHS2, CsF3'5'H1, CsDFR1, CsDFR2, and CsANR1, were significantly higher in YL than in the ST. In situ hybridization revealed that CsDFR1, CsDFR2, CsLAR, and CsANR1 were expressed in leaf primordia and YL but not in the apical meristem. These findings highlight the synthesis and accumulation patterns of catechins in different parts of the ST in C. sinensis, providing a theoretical basis for the assessment of synthesis, accumulation, and transfer patterns of catechins in tea plants.
[Mh] Termos MeSH primário: Camellia sinensis/metabolismo
Catequina/metabolismo
Folhas de Planta/metabolismo
[Mh] Termos MeSH secundário: Catequina/biossíntese
Catequina/genética
Cromatografia Líquida de Alta Pressão/métodos
Flavonoides/metabolismo
Regulação da Expressão Gênica de Plantas
Hibridização In Situ/métodos
Folhas de Planta/genética
Folhas de Planta/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Flavonoids); 35HDD3NRIE (flavan-3-ol); 8R1V1STN48 (Catechin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b03205



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