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Pesquisa : E01.370.225.500.620.670.325.680 [Categoria DeCS]
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  1 / 162 MEDLINE  
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[PMID]:24882625
[Au] Autor:Lalli C; van de Weghe P
[Ad] Endereço:Université de Rennes 1, UMR 6226 CNRS, Institut des Sciences Chimiques de Rennes, Equipe PNSCM, UFR des Sciences Biologiques et Pharmaceutiques, 2 Avenue du Prof. Léon Bernard, 35043 Rennes Cedex, France. claudia.lalli@univ-rennes1.fr pierre.van-de-weghe@univ-rennes1.fr.
[Ti] Título:Enantioselective Prins cyclization: BINOL-derived phosphoric acid and CuCl synergistic catalysis.
[So] Source:Chem Commun (Camb);50(56):7495-8, 2014 Jul 18.
[Is] ISSN:1364-548X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The first catalytic enantioselective Prins cyclization is disclosed. The reaction is catalyzed by the combination of a chiral BINOL-derived bis-phosphoric acid and CuCl. The process consists of a tandem Prins/Friedel-Crafts cyclization that affords the hexahydro-1H-benzo[f]isochromenes products with three new contiguous stereogenic centers in high yields, and good enantio- and excellent diastereoselectivities.
[Mh] Termos MeSH primário: Cobre/química
Naftóis/química
Ácidos Fosfóricos/química
Marcação in Situ com Primers/métodos
[Mh] Termos MeSH secundário: Catálise
Ciclização
Estereoisomerismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (BINOL, naphthol); 0 (Naphthols); 0 (Phosphoric Acids); 789U1901C5 (Copper); E4GA8884NN (phosphoric acid); S2QG84156O (cupric chloride)
[Em] Mês de entrada:1505
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140603
[St] Status:MEDLINE
[do] DOI:10.1039/c4cc02826k


  2 / 162 MEDLINE  
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[PMID]:24578229
[Au] Autor:Emad A; Bouchard EF; Lamoureux J; Ouellet A; Dutta A; Klingbeil U; Drouin R
[Ad] Endereço:Division of Genetics, Department of Pediatrics, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, Canada.
[Ti] Título:Validation of automatic scanning of microscope slides in recovering rare cellular events: application for detection of fetal cells in maternal blood.
[So] Source:Prenat Diagn;34(6):538-46, 2014 Jun.
[Is] ISSN:1097-0223
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Detection of rare fetal cells (FCs) in the maternal circulation could be used for non-invasive prenatal diagnosis. Considering that FCs in maternal blood are present in extremely low frequency, manual scanning is cumbersome, time-consuming, and unsuitable for clinical applications. As an alternative, we optimized a custom-made classifier for automatic detection of FCs. METHODS: Using MetaSystems' automated platform, we developed a robust detection algorithm and validated its efficiency on retrieval of rare XY cells in a pure population of XX cells. Slides were scanned for presence of predefined XY cells after fluorescence in situ hybridization (FISH) and primed in situ labeling (PRINS). Retrieval of FCs was also performed on samples from maternal blood. RESULTS: The efficiency of detection of rare XY cells was 88% using FISH (117/133) in comparison with 78% (53/68) with PRINS. FC frequencies per 1 mL of maternal blood ranged from 3 to 6 FCs in normal pregnancies versus 13 to 21 FCs in Down syndrome pregnancies. CONCLUSION: Automatic scanning was more efficient and consistent than manual scanning for detection of rare FCs and required considerably less operator time. Automatic scanning using FISH is more sensitive than that using PRINS. The study validates automatic scanning retrieval of FCs from maternal blood.
[Mh] Termos MeSH primário: Células Sanguíneas/citologia
Feto/citologia
Processamento de Imagem Assistida por Computador/métodos
Diagnóstico Pré-Natal/métodos
Marcação in Situ com Primers
[Mh] Termos MeSH secundário: Processamento Automatizado de Dados/métodos
Células Sanguíneas/patologia
Feminino
Testes Hematológicos/métodos
Seres Humanos
Hibridização in Situ Fluorescente
Cariotipagem/métodos
Gravidez
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; VALIDATION STUDIES
[Em] Mês de entrada:1504
[Cu] Atualização por classe:140603
[Lr] Data última revisão:
140603
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140301
[St] Status:MEDLINE
[do] DOI:10.1002/pd.4345


  3 / 162 MEDLINE  
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[PMID]:24569078
[Au] Autor:Kurogi Y; Matsuo Y; Mihara Y; Yagi H; Shigaki-Miyamoto K; Toyota S; Azuma Y; Igarashi M; Tani T
[Ad] Endereço:Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Chuo-ku, Kumamoto 860-8555, Japan.
[Ti] Título:Identification of a chemical inhibitor for nuclear speckle formation: implications for the function of nuclear speckles in regulation of alternative pre-mRNA splicing.
[So] Source:Biochem Biophys Res Commun;446(1):119-24, 2014 Mar 28.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Nuclear speckles are subnuclear structures enriched with RNA processing factors and poly (A)(+) RNAs comprising mRNAs and poly (A)(+) non-coding RNAs (ncRNAs). Nuclear speckles are thought to be involved in post-transcriptional regulation of gene expression, such as pre-mRNA splicing. By screening 3585 culture extracts of actinomycetes with in situ hybridization using an oligo dT probe, we identified tubercidin, an analogue of adenosine, as an inhibitor of speckle formation, which induces the delocalization of poly (A)(+) RNA and dispersion of splicing factor SRSF1/SF2 from nuclear speckles in HeLa cells. Treatment with tubercidin also decreased steady-state MALAT1 long ncRNA, thought to be involved in the retention of SRSF1/SF2 in nuclear speckles. In addition, we found that tubercidin treatment promoted exon skipping in the alternative splicing of Clk1 pre-mRNA. These results suggest that nuclear speckles play a role in modulating the concentration of splicing factors in the nucleoplasm to regulate alternative pre-mRNA splicing.
[Mh] Termos MeSH primário: Processamento Alternativo
Estruturas do Núcleo Celular/efeitos dos fármacos
Estruturas do Núcleo Celular/metabolismo
Precursores de RNA/metabolismo
[Mh] Termos MeSH secundário: Actinobacteria/química
Processamento Alternativo/efeitos dos fármacos
Processamento Alternativo/genética
Estruturas do Núcleo Celular/genética
Avaliação Pré-Clínica de Medicamentos
Éxons
Células HeLa
Seres Humanos
Modelos Biológicos
Proteínas Nucleares/metabolismo
Marcação in Situ com Primers
Proteínas Serina-Treonina Quinases/genética
Proteínas Tirosina Quinases/genética
Precursores de RNA/genética
RNA Longo não Codificante/genética
RNA Longo não Codificante/metabolismo
Proteínas de Ligação a RNA/metabolismo
Fatores de Processamento de Serina-Arginina
Tubercidina/isolamento & purificação
Tubercidina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (MALAT1 long non-coding RNA, human); 0 (Nuclear Proteins); 0 (RNA Precursors); 0 (RNA, Long Noncoding); 0 (RNA-Binding Proteins); 170974-22-8 (Serine-Arginine Splicing Factors); EC 2.7.1.- (Clk dual-specificity kinases); EC 2.7.10.1 (Protein-Tyrosine Kinases); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); M351LCX45Y (Tubercidin)
[Em] Mês de entrada:1406
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140227
[St] Status:MEDLINE


  4 / 162 MEDLINE  
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[PMID]:24279162
[Au] Autor:Bugno-Poniewierska M; Potocki L; Bladek B; Pawlina K; Wnuk M; Pietras M; Slota E
[Ad] Endereço:Laboratory of Genomics, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland. monika.bugno@izoo.krakow.pl
[Ti] Título:The use of primed in situ synthesis (PRINS) to analyze nucleolar organizer regions (NORs) and telomeric DNA sequences in the domestic chicken genome.
[So] Source:Folia Biol (Krakow);61(3-4):149-53, 2013.
[Is] ISSN:0015-5497
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:One of the most often analyzed avian genomes is the domestic chicken genome (Gallus domesticus) whose diploid number is 2n = 78. In the chicken karyotype, similarly to other birds, there is a group of microchromosomes for which the determination of morphology and banding pattern is impossible using classic cytogenetics methods. The aim of this study was to evaluate telomeric and rDNA repetitive sequences in the chicken genome by the PRINS technique as an alternative method to fluorescence in situ hybridization. This is the first report on the application of the PRINS method to locate these repetitive sequences in the chicken nuclei and metaphase chromosomes.
[Mh] Termos MeSH primário: Galinhas/genética
DNA/genética
Genoma
Região Organizadora do Nucléolo/genética
Marcação in Situ com Primers/métodos
Telômero/genética
[Mh] Termos MeSH secundário: Animais
Regulação da Expressão Gênica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-49-2 (DNA)
[Em] Mês de entrada:1312
[Cu] Atualização por classe:131127
[Lr] Data última revisão:
131127
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131128
[St] Status:MEDLINE


  5 / 162 MEDLINE  
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[PMID]:23085140
[Au] Autor:Capim SL; Carneiro PH; Castro PC; Barros MR; Marinho BG; Vasconcellos ML
[Ad] Endereço:Laboratório de Síntese Orgânica Medicinal da Paraíba, Departamento de Química, Universidade Federal da Paraíba, Campus I, João Pessoa, PB 58059-900, Brazil.
[Ti] Título:Design, Prins-cyclization reaction promoting diastereoselective synthesis of 10 new tetrahydropyran derivatives and in vivo antinociceptive evaluations.
[So] Source:Eur J Med Chem;58:1-11, 2012 Dec.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:We described in this article the very efficient 2,6-cis ou 2,4,6-cis diastereoselective synthesis (2 or 3 steps, 62-65% global yields) from Prins-cyclization reaction as synthetic key-step to tetrahydropyran rings construction of 10 new congeners compounds (3-12) designed from Naproxen structure. These tetrahydropyran derivatives were in vivo bioevaluated on antinociceptive effect in the acetic acid-induced abdominal writhing test, the tail-flick test, the rota-rod performance and open field tests. All new compounds showed greater antinociceptive activity compared to compound 1a, an analgesic tetrahydropyran derivative previously described by us. We can detach the high activity of tetrahydropyran derivative 10 which presented 87.5% inhibition (14% inhibition was presented by 1a) in the acetic acid-induced abdominal writhing test. Besides that the tail-flick tests indicate compounds 7 and 10 as the most actives. All these new compounds showed no toxicity in mice in all biologically studied models.
[Mh] Termos MeSH primário: Dor Abdominal/tratamento farmacológico
Analgésicos/uso terapêutico
Desenho de Drogas
Marcação in Situ com Primers
Piranos/uso terapêutico
[Mh] Termos MeSH secundário: Dor Abdominal/induzido quimicamente
Ácido Acético/metabolismo
Analgésicos/síntese química
Analgésicos/química
Animais
Ciclização
Relação Dose-Resposta a Droga
Masculino
Camundongos
Estrutura Molecular
Piranos/síntese química
Piranos/química
Estereoisomerismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Analgesics); 0 (Pyrans); Q40Q9N063P (Acetic Acid)
[Em] Mês de entrada:1305
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121023
[St] Status:MEDLINE


  6 / 162 MEDLINE  
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[PMID]:22714899
[Au] Autor:Goñi-de-Cerio F; Alvarez A; Lara-Celador I; Alvarez FJ; Alonso-Alconada D; Hilario E
[Ad] Endereço:Department of Cell Biology and Histology, School of Medicine and Dentistry, University of the Basque Country, Leioa, Vizcaya, Spain. goni@gaiker.es
[Ti] Título:Magnesium sulfate treatment decreases the initial brain damage alterations produced after perinatal asphyxia in fetal lambs.
[So] Source:J Neurosci Res;90(10):1932-40, 2012 Oct.
[Is] ISSN:1097-4547
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The aim of this work was to analyze the effect of MgSO(4) treatment in the brain after hypoxic-ischemic (HI) injury in premature fetal lambs. Injury was induced by partial occlusion of umbilical cord for 60 min, and then the preterm lambs (80-90% of gestation) were randomly assigned to one of the following groups: control group, in which the animals were managed by conventional mechanical ventilation for 3 hr; 3 hr postpartial cord occlusion (3-hr-PCO) group, in which injured animals were managed by ventilation and then sacrificed 3 hr after HI; and MgSO(4) group, in which animals received 400 mg/kg MgSO(4) for 20 min soon after HI was induced and were managed by ventilation for 3 hr. Brains were analyzed for apoptosis by TUNEL assay. Cell viability and intracellular state studies were assessed by flow cytometry. The delayed death index was significantly increased in the 3-hr-PCO group in comparison with control. Administration of MgSO(4) elicited a delay in cell death that was similar to that in the control group. The 3-hr-PCO group showed a significantly higher concentration of reactive oxygen species, mitochondrial damage, and intracellular calcium in comparison with control and MgSO(4) - treated groups. Our results suggest that MgSO(4) treatment might have potential therapeutic benefits after the HI event.
[Mh] Termos MeSH primário: Animais Recém-Nascidos/fisiologia
Asfixia/patologia
Dano Encefálico Crônico/patologia
Dano Encefálico Crônico/prevenção & controle
Sulfato de Magnésio/farmacologia
[Mh] Termos MeSH secundário: Animais
Anexina A5/metabolismo
Apoptose/efeitos dos fármacos
Cálcio/metabolismo
Artérias Carótidas/patologia
Membrana Celular/efeitos dos fármacos
Membrana Celular/ultraestrutura
Feminino
Feto/patologia
Corantes Fluorescentes
Imuno-Histoquímica
Marcação In Situ das Extremidades Cortadas
Potenciais da Membrana/efeitos dos fármacos
Membranas Mitocondriais/efeitos dos fármacos
Gravidez
Marcação in Situ com Primers
Espécies Reativas de Oxigênio/metabolismo
Rodamina 123
Ovinos
Medula Espinal/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Annexin A5); 0 (Fluorescent Dyes); 0 (Reactive Oxygen Species); 1N3CZ14C5O (Rhodamine 123); 7487-88-9 (Magnesium Sulfate); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1301
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120621
[St] Status:MEDLINE
[do] DOI:10.1002/jnr.23091


  7 / 162 MEDLINE  
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[PMID]:22678791
[Au] Autor:Luo X; Ding X; Chen L; Quan Q
[Ad] Endereço:Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, Institute of Medical Genetics, College of Life Science, Sichuan University, Chengdu, Sichuan 610041, P. R. China.
[Ti] Título:[Rapid detection of SOX2 gene by primed in situ labeling].
[So] Source:Zhonghua Yi Xue Yi Chuan Xue Za Zhi;29(3):289-92, 2012 Jun.
[Is] ISSN:1003-9406
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To rapidly detect SOX2 gene using primed in situ labeling (PRINS). METHODS: Human peripheral blood samples were cultured using an optimized method. Sequence of the SOX2 gene was amplified in situ with biotin-labeled specific primers and processed with a tyramide signal amplification (TSA) biotin system. Subsequently, fluorescence-stained signal was detected by streptavidin-Texas red. For the control group, MCF-10F cells were transfected with Lentivirus hSox2. RESULTS: By VideoTesT-FISH software analysis, the long arm of chromosome 3 in the experimental group showed a specific red fluorescence signal, whilst the control samples showed no specific signals for SOX2. Transfected MCF-10F cells showed various efficiency of SOX2 gene integration. CONCLUSION: PRINS utilizes a highly sensitive in situ PCR technique combined with fluorescence labeled oligodeoxynucleotides can synthesize probes in situ, thus greatly reducing the cost of probe and time for detection. It can facilitate identification and classification of induced pluripotent stem cells, and has many potential applications in this prospect.
[Mh] Termos MeSH primário: Marcação in Situ com Primers/métodos
Fatores de Transcrição SOXB1/química
[Mh] Termos MeSH secundário: Seres Humanos
Hibridização in Situ Fluorescente/métodos
Masculino
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (SOX2 protein, human); 0 (SOXB1 Transcription Factors)
[Em] Mês de entrada:1209
[Cu] Atualização por classe:120608
[Lr] Data última revisão:
120608
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120609
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.1003-9406.2012.03.009


  8 / 162 MEDLINE  
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[PMID]:22648284
[Au] Autor:Mozdarani H; Ghoraeian P
[Ad] Endereço:Department of Medical Genetics, Faculty of Medical Sciences , Tarbiat Modares University, Tehran, Iran. mozdarah@modares.ac.ir
[Ti] Título:Efficient combined FISH and PRINS technique for detection of DAZ microdeletion in human sperm.
[So] Source:J Assist Reprod Genet;29(9):979-84, 2012 Sep.
[Is] ISSN:1573-7330
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Intracytoplasmic sperm injection (ICSI) now offers an effective therapeutic option for men with male infertility and is believed to allow transmission of genetically determined infertility to the male offspring. Transmission of DAZ (Deleted in Azoospermia) microdeletion is one of the major concerns for oligo and severe oligozoospermia patients. Screening of the Y chromosome microdeletion in the diagnostic work-up of infertile men is mainly done using polymerase chain reaction (PCR) on blood leukocytes. However, there are evidences showing that presence of DAZ in somatic cells might not be indicative of its presence in germ cell lineage. In this report we are going to describe a combined Primed in situ labeling (PRINS) and fluorescence in situ hybridization (FISH) technique to show the localization of DAZ gene as well as Y chromosome centromere on sperm nuclei. PRINS is a combination of FISH and in situ polymerization provides another approach for in situ chromosomal detection. In the present study the PRINS primers specific for DAZ genes and traditional direct labeled centromere FISH probes for Y and X chromosomes were used in order to simultaneously detect DAZ genes and sex chromosome aneuploidy in sperm samples.
[Mh] Termos MeSH primário: Hibridização in Situ Fluorescente/métodos
Marcação in Situ com Primers/métodos
Proteínas de Ligação a RNA/genética
Transtornos do Cromossomo Sexual no Desenvolvimento Sexual/genética
Espermatozoides/citologia
[Mh] Termos MeSH secundário: Adulto
Núcleo Celular/genética
Centrômero/genética
Deleção Cromossômica
Cromossomos Humanos Y/genética
Sondas de DNA
Proteína 1 Suprimida em Azoospermia
Testes Genéticos
Seres Humanos
Infertilidade Masculina
Masculino
Oligospermia/genética
Reprodutibilidade dos Testes
Análise do Sêmen/métodos
Sensibilidade e Especificidade
Aberrações dos Cromossomos Sexuais
Contagem de Espermatozoides
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DAZ1 protein, human); 0 (DNA Probes); 0 (Deleted in Azoospermia 1 Protein); 0 (RNA-Binding Proteins)
[Em] Mês de entrada:1303
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120601
[St] Status:MEDLINE
[do] DOI:10.1007/s10815-012-9805-z


  9 / 162 MEDLINE  
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[PMID]:22502695
[Au] Autor:Salimi M; Mozdarani H; Majidzadeh-A K
[Ad] Endereço:Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
[Ti] Título:Efficacy of primed in situ labelling in determination of HER-2 gene amplification and CEN-17 status in breast cancer tissue.
[So] Source:Asian Pac J Cancer Prev;13(1):329-37, 2012.
[Is] ISSN:2476-762X
[Cp] País de publicação:Thailand
[La] Idioma:eng
[Ab] Resumo:Considerable attention has been given to the accuracy of HER-2 testing and the correlation between the results of different testing methods. This interest reflects the growing importance of HER-2 status in the management of patients with breast cancer. In this study the detection of HER-2 gene and centromere 17 status was evaluated using dual-colour primed in situ labelling (PRINS) in comparison with fluorescence in situ hybridization (FISH). These two methods were evaluated on a series of 27 formalin fixed paraffin embedded breast carcinoma tumours, previously tested for protein overexpression by HercepTest (grouped into Hercep 1+/0, 2+ and 3+). HER-2 gene amplification (ratio ≥ 2.2) by PRINS was found in 3:3, 6:21 and 0:3 in IHC 3+, 2+ and 1+/0 cases, respectively. Comparing FISH and IHC (immunohistochemistry), showed the same results as for PRINS and IHC. Chromosome 17 aneusomy was found in 10 of 21 IHC 2+ cases (47.6%), of which 1 (10%) showed hypodisomy (chromosome 17 copy number per cell ≤ 1.75), 7 (70%) showed low polysomy (chromosome 17 copy number per cell=2.26 - 3.75) and 2 (20%) showed high polysomy (chromosome 17 copy number per cell ≥ 3.76). The overall concordance of detection of HER-2 gene amplification by FISH and PRINS was 100% (27:27). Furthermore, both the level of HER-2 amplification and copy number of CEN17 analysis results correlated well between the two methods. In conclusion, PRINS is a reliable, reproducible technique and in our opinion can be used as an additional test to determine HER-2 status in breast tumours.
[Mh] Termos MeSH primário: Neoplasias da Mama/genética
Aberrações Cromossômicas
Cromossomos Humanos Par 17/genética
Amplificação de Genes
Marcação in Situ com Primers/métodos
Receptor ErbB-2/genética
[Mh] Termos MeSH secundário: Neoplasias da Mama/metabolismo
Neoplasias da Mama/patologia
Centrossomo
Feminino
Regulação Neoplásica da Expressão Gênica
Seres Humanos
Técnicas Imunoenzimáticas
Hibridização in Situ Fluorescente
Inclusão em Parafina
Marcação in Situ com Primers/utilização
Prognóstico
Receptor ErbB-2/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 2.7.10.1 (Receptor, ErbB-2)
[Em] Mês de entrada:1208
[Cu] Atualização por classe:170308
[Lr] Data última revisão:
170308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120417
[St] Status:MEDLINE


  10 / 162 MEDLINE  
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[PMID]:22395136
[Au] Autor:Nili HA; Mozdarani H; Pellestor F
[Ad] Endereço:Dept. of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box: 14115-111, Tehran, Iran.
[Ti] Título:Impact of DNA damage on the frequency of sperm chromosomal aneuploidy in normal and subfertile men.
[So] Source:Iran Biomed J;15(4):122-9, 2011.
[Is] ISSN:2008-823X
[Cp] País de publicação:Iran
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Various frequencies of sperm aneuploidy are reported in sperms of subfertile patients compared to normal individuals. Moreover, sperm DNA damage is shown to be associated with male infertility. In this study, the rate of DNA damage and frequencies of aneuploidy in sperms of subfertile patients was investigated. METHODS: Semen samples were obtained from healthy normal and subfertile (oligozoospermia, asthenozoospermia, and oligoasthenozoospermia) men. The frequency of aneuploidy was assessed using primed in situ labeling (PRINS) analysis with specific primers for chromosomes 18, 21, X, and Y. Sperm DNA damage was assessed using alkaline comet assay. RESULTS: The mean frequencies of disomy for the patients were significantly higher than normal for all chromosomes (P < 0.01). The extent of DNA damage in sperms of subfertiles was significantly higher than in normal individuals (P < 0.001). The obtained results indicated that higher rate of DNA damages led to higher frequency of chromosomal disomy except for asthenozoospermia samples which exhibited higher rate of DNA damage and lower frequency of chromosomal disomy. CONCLUSIONS: These results demonstrate that men with oligozoospermia and oligoasthenozoospermia have an elevated risk for chromosome abnormalities in their sperm, particularly sex chromosomes. DNA damage might be involved in the process of malsegregation of chromosomes.
[Mh] Termos MeSH primário: Aneuploidia
Cromossomos Humanos/genética
Dano ao DNA
Infertilidade Masculina/genética
Infertilidade Masculina/patologia
Espermatozoides/metabolismo
[Mh] Termos MeSH secundário: Adulto
Núcleo Celular/metabolismo
Demografia
Seres Humanos
Masculino
Marcação in Situ com Primers
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1209
[Cu] Atualização por classe:170114
[Lr] Data última revisão:
170114
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120308
[St] Status:MEDLINE



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