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  1 / 11071 MEDLINE  
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[PMID]:29360866
[Au] Autor:Cui B; Wang C; Zhao X; Yao J; Zeng Z; Wang Y; Sun C; Liu G; Cui H
[Ad] Endereço:Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, China.
[Ti] Título:Characterization and evaluation of avermectin solid nanodispersion prepared by microprecipitation and lyophilisation techniques.
[So] Source:PLoS One;13(1):e0191742, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Poorly water-soluble and photosensitive pesticide compounds are difficult to formulate as solvent-free nanoformulations with high efficacy. A avermectin solid nanodispersion with a mean particle size of 188 nm was developed by microprecipitation and lyophilisation techniques. The suspensibility and wetting time of the solid nanodispersion in water were 99.8% and 13 s, respectively, superior to those of conventional water dispersible granules and wettable powders. The anti-photolysis performance of the nanoformulation was twice that of the technical material, and the biological activity against diamondback moths was more than 1.5 times that of the conventional solid formulations while taking LC 50 as the evaluation index. Moreover, the formulation composition substantially decreased the surfactant content and avoided organic solvents. Microprecipitation combined with lyophilisation is an easy and promising method to construct solid nanoformulations for pesticides with poor water solubility and environmental sensitivity. The application of the highly effective solid nanodispersion in crop production will have a great potential in reducing chemical residues and environmental pollution.
[Mh] Termos MeSH primário: Inseticidas/química
Ivermectina/análogos & derivados
[Mh] Termos MeSH secundário: Bioensaio
Liofilização
Ivermectina/química
Nanotecnologia
Solubilidade
Água/química
Molhabilidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Insecticides); 059QF0KO0R (Water); 70288-86-7 (Ivermectin); 73989-17-0 (avermectin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180124
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191742


  2 / 11071 MEDLINE  
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[PMID]:29244815
[Au] Autor:Liu CC; You CH; Wang PJ; Yu JS; Huang GJ; Liu CH; Hsieh WC; Lin CC
[Ad] Endereço:Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan.
[Ti] Título:Analysis of the efficacy of Taiwanese freeze-dried neurotoxic antivenom against Naja kaouthia, Naja siamensis and Ophiophagus hannah through proteomics and animal model approaches.
[So] Source:PLoS Negl Trop Dis;11(12):e0006138, 2017 12.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In Southeast Asia, envenoming resulting from cobra snakebites is an important public health issue in many regions, and antivenom therapy is the standard treatment for the snakebite. Because these cobras share a close evolutionary history, the amino acid sequences of major venom components in different snakes are very similar. Therefore, either monovalent or polyvalent antivenoms may offer paraspecific protection against envenomation of humans by several different snakes. In Taiwan, a bivalent antivenom-freeze-dried neurotoxic antivenom (FNAV)-against Bungarus multicinctus and Naja atra is available. However, whether this antivenom is also capable of neutralizing the venom of other species of snakes is not known. Here, to expand the clinical application of Taiwanese FNAV, we used an animal model to evaluate the neutralizing ability of FNAV against the venoms of three common snakes in Southeast Asia, including two 'true' cobras Naja kaouthia (Thailand) and Naja siamensis (Thailand), and the king cobra Ophiophagus hannah (Indonesia). We further applied mass spectrometry (MS)-based proteomic techniques to characterize venom proteomes and identify FNAV-recognizable antigens in the venoms of these Asian snakes. Neutralization assays in a mouse model showed that FNAV effectively neutralized the lethality of N. kaouthia and N. siamensis venoms, but not O. hannah venom. MS-based venom protein identification results further revealed that FNAV strongly recognized three-finger toxin and phospholipase A2, the major protein components of N. kaouthia and N. siamensis venoms. The characterization of venom proteomes and identification of FNAV-recognizable venom antigens may help researchers to further develop more effective antivenom designed to block the toxicity of dominant toxic proteins, with the ultimate goal of achieving broadly therapeutic effects against these cobra snakebites.
[Mh] Termos MeSH primário: Antídotos/farmacologia
Antivenenos/farmacologia
Venenos Elapídicos/química
Proteoma
Mordeduras de Serpentes/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Antídotos/química
Antivenenos/química
Cromatografia Líquida de Alta Pressão
Cromatografia Líquida
Modelos Animais de Doenças
Venenos Elapídicos/envenenamento
Liofilização
Seres Humanos
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Testes de Neutralização
Taiwan
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antidotes); 0 (Antivenins); 0 (Elapid Venoms); 0 (Proteome)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0006138


  3 / 11071 MEDLINE  
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[PMID]:29241319
[Au] Autor:Chen H; Huang J; Shi X; Li Y; Liu Y
[Ad] Endereço:School of Food and Biological Engineering, Shaanxi University of Science and Technology Xi?an,China.
[Ti] Título:Effects of six substances on the growth and freeze-drying of Lactobacillus delbrueckii subsp. bulgaricus.
[So] Source:Acta Sci Pol Technol Aliment;16(4):403-412, 2017 Oct-Dec.
[Is] ISSN:1898-9594
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The efficacy of Lactobacillus delbrueckii subsp. bulgaricus as starter cultures for the dairy industry depends largely on the number of viable and active cells. Freeze-drying is the most convenient and successful method to preserve the bacterial cells. However, not all strains survived during freeze-drying. METHODS: The effects of six substances including NaCl, sorbitol, mannitol, mannose, sodium glutamate, betaine added to the MRS medium on the growth and freeze-drying survival rate and viable counts of Lb. delbrueckii subsp. bulgaricus were studied through a single-factor test and Plackett-Burman design. Subsequently, the optimum freeze-drying conditions of Lb. delbrueckii subsp. bulgaricus were determined. RESULTS: Lb. delbrueckii subsp. bulgaricus survival rates were up to the maximum of 42.7%, 45.4%, 23.6%, while the concentrations of NaCl, sorbitol, sodium glutamate were 0.6%, 0.15%, 0.09%, respectively. In the optimum concentration, the viable counts in broth is 6.1, 6.9, 5.13 (×108 CFU/mL), respectively; the viable counts in freeze-drying power are 3.09, 5.2, 2.7 (×1010 CFU/g), respectively. CONCLUSIONS: Three antifreeze factors including NaCl, sorbitol, sodium glutamate have a positive effect on the growth and freeze-drying of Lb. delbrueckii subsp. bulgaricus. The results are beneficial for developing Lb. delbrueckii subsp. bulgaricus.
[Mh] Termos MeSH primário: Crioprotetores/farmacologia
Aditivos Alimentares/farmacologia
Liofilização
Lactobacillus delbrueckii
[Mh] Termos MeSH secundário: Betaína/farmacologia
Lactobacillus delbrueckii/efeitos dos fármacos
Lactobacillus delbrueckii/crescimento & desenvolvimento
Manitol/farmacologia
Viabilidade Microbiana
Cloreto de Sódio/farmacologia
Glutamato de Sódio/farmacologia
Sorbitol/farmacologia
Iogurte/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cryoprotective Agents); 0 (Food Additives); 3OWL53L36A (Mannitol); 3SCV180C9W (Betaine); 451W47IQ8X (Sodium Chloride); 506T60A25R (Sorbitol); W81N5U6R6U (Sodium Glutamate)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.17306/J.AFS.0512


  4 / 11071 MEDLINE  
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[PMID]:28870733
[Au] Autor:Stenger B; Gerber A; Bernhardt R; Hannemann F
[Ad] Endereço:Institute of Biochemistry, Saarland University, Saarbrücken, Germany.
[Ti] Título:Functionalized poly(3-hydroxybutyric acid) bodies as new in vitro biocatalysts.
[So] Source:Biochim Biophys Acta;1866(1):52-59, 2018 01.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Cytochromes P450 play a key role in the drug and steroid metabolism in the human body. This leads to a high interest in this class of proteins. Mammalian cytochromes P450 are rather delicate. Due to their localization in the mitochondrial or microsomal membrane, they tend to aggregate during expression and purification and to convert to an inactive form so that they have to be purified and stored in complex buffers. The complex buffers and low storage temperatures, however, limit the feasibility of fast, automated screening of the corresponding cytochrome P450-effector interactions, which are necessary to study substrate-protein and inhibitor-protein interactions. Here, we present the production and isolation of functionalized poly(3-hydroxybutyrate) granules (PHB bodies) from Bacillus megaterium MS941 strain. In contrast to the expression in Escherichia coli, where mammalian cytochromes P450 are associated to the cell membrane, when CYP11A1 is heterologously expressed in Bacillus megaterium, it is located on the PHB bodies. The surface of these particles provides a matrix for immobilization and stabilization of the CYP11A1 during the storage of the protein and substrate conversion. It was demonstrated that the PHB polymer basis is inert concerning the performed conversion. Immobilization of the CYP11A1 onto the PHB bodies allows freeze-drying of the complex without significant decrease of the CYP11A1 activity. This is the first lyophilization of a mammalian cytochrome P450, which allows storage over more than 18days at 4°C instead of storage at -80°C. In addition, we were able to immobilize the cytochrome P450 on the PHB bodies in vitro. In this case the expression of the protein is separated from the production of the immobilization matrix, which widens the application of this method. This article is part of a Special Issue entitled: Cytochrome P450 biodiversity and biotechnology, edited by Erika Plettner, Gianfranco Gilardi, Luet Wong, Vlada Urlacher, Jared Goldstone.
[Mh] Termos MeSH primário: Ácido 3-Hidroxibutírico/química
Bacillus megaterium/genética
Biotecnologia/métodos
Enzima de Clivagem da Cadeia Lateral do Colesterol/química
Proteínas Imobilizadas/biossíntese
Proteínas Mitocondriais/biossíntese
[Mh] Termos MeSH secundário: Ácido 3-Hidroxibutírico/biossíntese
Animais
Bacillus megaterium/enzimologia
Biocatálise
Bovinos
Colesterol/química
Colesterol/metabolismo
Enzima de Clivagem da Cadeia Lateral do Colesterol/genética
Enzima de Clivagem da Cadeia Lateral do Colesterol/metabolismo
Grânulos Citoplasmáticos/química
Liofilização
Expressão Gênica
Proteínas Imobilizadas/química
Proteínas Imobilizadas/genética
Proteínas Mitocondriais/química
Proteínas Mitocondriais/genética
Pregnenolona/biossíntese
Pregnenolona/química
Refrigeração
Transgenes
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Immobilized Proteins); 0 (Mitochondrial Proteins); 73R90F7MQ8 (Pregnenolone); 97C5T2UQ7J (Cholesterol); EC 1.14.15.6 (Cholesterol Side-Chain Cleavage Enzyme); TZP1275679 (3-Hydroxybutyric Acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170906
[St] Status:MEDLINE


  5 / 11071 MEDLINE  
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[PMID]:29025651
[Au] Autor:Feng S; He F; Ye J
[Ad] Endereço:School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
[Ti] Título:Hierarchically porous structure, mechanical strength and cell biological behaviors of calcium phosphate composite scaffolds prepared by combination of extrusion and porogen burnout technique and enhanced by gelatin.
[So] Source:Mater Sci Eng C Mater Biol Appl;82:217-224, 2018 Jan 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this study, hierarchically porous calcium phosphate scaffolds (HTCP) with unidirectional pores, transversely interconnected pores, and micropores were fabricated by the combination of extrusion and porogen burnout technique. Gelatin was incorporated into the HTCP scaffolds by vacuum-impregnation of gelatin solution and subsequent freeze-drying. The phase composition, microstructure, physical and cytobiological properties were analyzed. The results showed that the HTCP scaffolds were composed of ß-tricalcium phosphate with minor hydroxyapatite. The HTCP scaffolds had unidirectional pores (~400µm), transversely interconnected pores (~130µm) and micropores (~1µm). The incorporation of gelatin significantly increased the compressive strength, toughness, and cell seeding of the HTCP scaffolds. The composite scaffolds showed excellent cytocompatibility. The hierarchically porous calcium phosphate composite scaffolds may have potential application prospects in bone tissue engineering.
[Mh] Termos MeSH primário: Materiais Biocompatíveis/química
Fosfatos de Cálcio/química
Gelatina/química
Engenharia Tecidual/métodos
Tecidos Suporte/química
[Mh] Termos MeSH secundário: Animais
Materiais Biocompatíveis/farmacologia
Células da Medula Óssea/citologia
Adesão Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Células Cultivadas
Força Compressiva
Liofilização
Células Mesenquimais Estromais/citologia
Células Mesenquimais Estromais/metabolismo
Camundongos
Porosidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Calcium Phosphates); 9000-70-8 (Gelatin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171014
[St] Status:MEDLINE


  6 / 11071 MEDLINE  
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[PMID]:29191190
[Au] Autor:Xie L; Zhong X; Liu D; Liu L; Xia Z
[Ad] Endereço:College of Horticulture and Landscape Architecture, Hunan Agricultural University, Changsha, China.
[Ti] Título:The effects of freeze-dried Ganoderma lucidum mycelia on a recurrent oral ulceration rat model.
[So] Source:BMC Complement Altern Med;17(1):511, 2017 Dec 01.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Conventional scientific studies had supported the use of polysaccharides and ß-glucans from a number of fungi, including Ganoderma lucidum for the treatment of recurrent oral ulceration (ROU). Our aim of the present study was to evaluate whether freeze-dried powder from G. lucidum mycelia (FDPGLM) prevents ROU in rats. METHODS: A Sprague-Dawley (SD) rat model with ROU was established by autoantigen injection. The ROU rats were treated with three different dosages of FDPGLM and prednisone acetate (PA), and their effects were evaluated according to the clinical therapeutic evaluation indices of ROU. RESULTS: High-dose FDPGLM induced significantly prolonged total intervals and a reduction in the number of ulcers and ulcer areas, thereby indicating that the treatment was effective in preventing ROU. Enzyme-linked immunosorbent assay (ELISA) showed that high-dose FDPGLM significantly enhanced the serum transforming growth factor-ß1 (TGF-ß1) levels, whereas reduced those of interleukin-6 (IL-6) and interleukin-17 (IL-17). Flow cytometry (FCM) showed that the proportion of CD4 CD25 Foxp3 (forkhead box P3) regulatory T cells (Tregs) significantly increased by 1.5-fold in the high-dose FDPGLM group compared to that in the rat model group (P < 0.01). The application of middle- and high-dose FDPGLM also resulted in the upregulation of Foxp3 and downregulation of retinoid-related orphan receptor gamma t(RORγt) mRNA. CONCLUSION: High-dose FDPGLM possibly plays a role in ROU by promoting CD4 CD25 Foxp3 Treg and inhibiting T helper cell 17 differentiation. This study also shows that FDPGLM may be potentially used as a complementary and alternative medicine treatment scheme for ROU.
[Mh] Termos MeSH primário: Produtos Biológicos/uso terapêutico
Ganoderma/química
Micélio/química
Úlceras Orais/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Produtos Biológicos/química
Produtos Biológicos/farmacologia
Citocinas/sangue
Modelos Animais de Doenças
Fatores de Transcrição Forkhead/genética
Fatores de Transcrição Forkhead/metabolismo
Liofilização
Mucosa Bucal/química
Mucosa Bucal/patologia
Prednisona/farmacologia
Prednisona/uso terapêutico
Ratos
Ratos Sprague-Dawley
Recidiva
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biological Products); 0 (Cytokines); 0 (Forkhead Transcription Factors); 0 (Foxp3 protein, rat); VB0R961HZT (Prednisone)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171220
[Lr] Data última revisão:
171220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-2021-8


  7 / 11071 MEDLINE  
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[PMID]:29176824
[Au] Autor:Mendonça-da-Silva I; Magela Tavares A; Sachett J; Sardinha JF; Zaparolli L; Gomes Santos MF; Lacerda M; Monteiro WM
[Ad] Endereço:Escola Superior de Saúde, Universidade do Estado do Amazonas, Manaus, Amazonas, Brazil.
[Ti] Título:Safety and efficacy of a freeze-dried trivalent antivenom for snakebites in the Brazilian Amazon: An open randomized controlled phase IIb clinical trial.
[So] Source:PLoS Negl Trop Dis;11(11):e0006068, 2017 Nov.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In tropical areas, a major concern regarding snakebites treatment effectiveness relates to the failure in liquid antivenom (AV) distribution due to the lack of an adequate cold chain in remote areas. To minimize this problem, freeze-drying has been suggested to improve AV stability. METHODS AND FINDINGS: This study compares the safety and efficacy of a freeze-dried trivalent antivenom (FDTAV) and the standard liquid AV provided by the Brazilian Ministry of Health (SLAV) to treat Bothrops, Lachesis and Crotalus snakebites. This was a prospective, randomized, open, phase IIb trial, carried out from June 2005 to May 2008 in the Brazilian Amazon. Primary efficacy endpoints were the suppression of clinical manifestations and return of hemostasis and renal function markers to normal ranges within the first 24 hours of follow-up. Primary safety endpoint was the presence of early adverse reactions (EAR) in the first 24 hours after treatment. FDTAV thermal stability was determined by estimating AV potency over one year at 56°C. Of the patients recruited, 65 and 51 were assigned to FDTAV and SLAV groups, respectively. Only mild EARs were reported, and they were not different between groups. There were no differences in fibrinogen (p = 0.911) and clotting time (p = 0.982) recovery between FDTAV and SLAV treated groups for Bothrops snakebites. For Lachesis and Crotalus snakebites, coagulation parameters and creatine phosphokinase presented normal values 24 hours after AV therapy for both antivenoms. CONCLUSIONS/SIGNIFICANCE: Since promising results were observed for efficacy, safety and thermal stability, our results indicate that FDTAV is suitable for a larger phase III trial. TRIAL REGISTRATION: ISRCTNregistry: ISRCTN12845255; DOI: 10.1186/ISRCTN12845255 (http://www.isrctn.com/ISRCTN12845255).
[Mh] Termos MeSH primário: Antivenenos/administração & dosagem
Bothrops
Crotalus
Mordeduras de Serpentes/terapia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Animais
Antivenenos/efeitos adversos
Coagulação Sanguínea
Brasil
Criança
Pré-Escolar
Feminino
Fibrinogênio/análise
Liofilização
Seres Humanos
Lactente
Recém-Nascido
Masculino
Meia-Idade
Estudos Prospectivos
Resultado do Tratamento
Adulto Jovem
[Pt] Tipo de publicação:CLINICAL TRIAL, PHASE II; JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Antivenins); 9001-32-5 (Fibrinogen)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0006068


  8 / 11071 MEDLINE  
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[PMID]:28466491
[Au] Autor:Politowicz J; Lech K; Sánchez-Rodríguez L; Szumny A; Carbonell-Barrachina ÁA
[Ad] Endereço:The Faculty of Food Science, Department of Chemistry, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland.
[Ti] Título:Volatile composition and sensory profile of Cantharellus cibarius Fr. as affected by drying method.
[So] Source:J Sci Food Agric;97(15):5223-5232, 2017 Dec.
[Is] ISSN:1097-0010
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In this work, the influence of different drying methods on the aroma composition and sensory quality of chanterelle mushrooms (Cantharellus cibarius Fr.) was evaluated. The drying methods tested were convective drying (CD), freeze drying (FD), vacuum microwave drying (VMD) and a combination of convective pre-drying and vacuum microwave finish drying (CPD-VMFD). RESULTS: Analyses of fresh and dried chanterelle samples by HS-SPME and GC/MS and GC-FID showed the presence of 39 volatile compounds at different concentrations. The most abundant compounds in fresh chanterelle were 1-hexanol (33.4 µg per 100 g dry basis (db)), 1-octen-3-ol (80.2 µg per 100 g db) and 2-octen-1-ol (19.3 µg per 100 g db). The results showed that fresh and dried chanterelle contained very low levels of aroma compounds; however, the highest contents of volatile compounds were found in samples after (i) CD at 80 °C (129 µg per 100 g db), (ii) CPD-VMFD at 70 °C-480/240 W (136 µg per 100 g db) and (iii) CPD-VMFD at 80 °C-480/240 W (136 µg per 100 g db). CONCLUSION: The best dehydration methods, which resulted in high contents of volatile compounds and appropriate sensory quality, according to descriptive sensory analysis and PCA tools, were CD at 70 and 80 °C. Besides, these methods led to spongy dried mushrooms with high intensities of fresh, mushroom ID, with proper color and without intense shrinkage. © 2017 Society of Chemical Industry.
[Mh] Termos MeSH primário: Agaricales/química
Conservação de Alimentos/métodos
Verduras/química
Compostos Orgânicos Voláteis/química
[Mh] Termos MeSH secundário: Adulto
Feminino
Liofilização
Cromatografia Gasosa-Espectrometria de Massas
Seres Humanos
Masculino
Meia-Idade
Odorantes/análise
Paladar
Adulto Jovem
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Volatile Organic Compounds)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171215
[Lr] Data última revisão:
171215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1002/jsfa.8406


  9 / 11071 MEDLINE  
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[PMID]:27771082
[Au] Autor:Shao Y; Wang Z; Bao Q; Zhang H
[Ad] Endereço:College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi' an, Shaanxi, P. R. China, 710119.
[Ti] Título:Application of propidium monoazide quantitative real-time PCR to quantify the viability of Lactobacillus delbrueckii ssp. bulgaricus.
[So] Source:J Dairy Sci;99(12):9570-9580, 2016 Dec.
[Is] ISSN:1525-3198
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, a combination of propidium monoazide (PMA) and quantitative real-time PCR (qPCR) was used to develop a method to determine the viability of cells of Lactobacillus delbrueckii ssp. bulgaricus ND02 (L. bulgaricus) that may have entered into a viable but nonculturable state. This can happen due to its susceptibility to cold shock during lyophilization and storage. Propidium monoazide concentration, PMA incubation time, and light exposure time were optimized to fully exploit the PMA-qPCR approach to accurately assess the total number of living L. bulgaricus ND02. Although PMA has little influence on living cells, when concentrations of PMA were higher than 30µg/mL the number of PCR-positive living bacteria decreased from 10 to 10 cfu/mL in comparison with qPCR enumeration. Mixtures of living and dead cells were used as method verification samples for enumeration by PMA-qPCR, demonstrating that this method was feasible and effective for distinguishing living cells of L. bulgaricus when mixed with a known number of dead cells. We suggest that several conditions need to be studied further before PMA-qPCR methods can be accurately used to distinguish living from dead cells for enumeration under more realistic sampling situations. However, this research provides a rapid way to enumerate living cells of L. bulgaricus and could be used to optimize selection of cryoprotectants in the lyophilization process and develop technologies for high cell density cultivation and optimal freeze-drying processes.
[Mh] Termos MeSH primário: Lactobacillus delbrueckii
Reação em Cadeia da Polimerase em Tempo Real
[Mh] Termos MeSH secundário: Animais
Azidas
Bactérias
Liofilização
Viabilidade Microbiana
Propídio
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Azides); 36015-30-2 (Propidium)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171204
[Lr] Data última revisão:
171204
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:28930960
[Au] Autor:Shlaifer A; Siman-Tov M; Radomislensky I; Peleg K; Shina A; Baruch EN; Glassberg E; Yitzhak A; ITG*
[Ad] Endereço:From the The Israel Defense Forces Medical Corps (S.A., S.A., B.N.A., G.E., Y.A.), Tel-Hashomer; The Orthopedic Division (S.A.), Tel Aviv Sourasky Medical Center, Tel Aviv; Israel National Center for Trauma and Emergency Medicine (S.-T.M., R.I., P.K.), Gertner Institute for Epidemiology and Public Health Policy, Tel-Hashomer; Department of Disaster Management (P.K.), School of Public Health, Tel Aviv University, Tel Aviv; The Pinchas Bornstein Talpiot Medical Leadership Program (S.A.), The Sheba Medical Center; The Ella Lemelbaum Institute of Immuno-Oncology (B.N.A.), Institute of Oncology, The Sheba Medical Center, Ramat Gan; Sackler Faculty of Medicine (B.N.A.), Tel Aviv University, Tel Aviv, Israel; and The Department of Surgery at Uniformed Services (G.E.), University of the Health Sciences, Bethesda, MD.
[Ti] Título:Prehospital administration of freeze-dried plasma, is it the solution for trauma casualties?
[So] Source:J Trauma Acute Care Surg;83(4):675-682, 2017 Oct.
[Is] ISSN:2163-0763
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Hemorrhage is the leading cause of possible preventable death in the battlefield. There is an increasing evidence for the effectiveness of blood component therapy in general, and plasma infusion in particular but their use is less applicable in the prehospital setting due to logistic difficulties. Israeli Defense Force has implemented the use of freeze-dried plasma (FDP) at the point of injury (POI), this adoption of FDP use entailed doubts regarding the feasibility and effectiveness of this practice. In this article, we present our experience with the use of FDP at the POI and prehospital setting regarding the feasibility, safety, adverse reactions, and adherence to clinical practice guidelines. METHODS: This is a descriptive retrospective cohort study based on all casualties receiving FDP during January 2013 to June 2016. The study describes the injury, treatment, and outcome characteristics from POI until hospital discharge. RESULTS: During the study period, 109 casualties received FDP. The majority were men, aged 18 years to 35 years. Multiple severe injuries were found in almost half of the casualties, 78% had penetrating injury, and more than half were involved in a multicasualty event. Eighty-three percent were treated with one unit of FDP, 13% with two units, and 4% casualties with three units, nine patients (8.2%) were also treated in the prehospital setting with packed red blood cells. Fifty-seven percent fulfilled at least one criterion for the administration of FDP. Lifesaving interventions were required in 64%. In five (4.6%) cases, there were difficulties with FDP administration. Side effects were reported in one female patient. CONCLUSION: This study supports the usage feasibility of FDP at the POI and in the prehospital setting. Further adjustment of the clinical practice guidelines is required basing it not only on pathophysiologic parameters but also on clinical judgment. Further investigation of the available data is required to learn about the effectiveness of FDP at POI. LEVEL OF EVIDENCE: Retrospective case series study, level IV.
[Mh] Termos MeSH primário: Transfusão de Componentes Sanguíneos
Serviços Médicos de Emergência
Ferimentos e Lesões/terapia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Estudos de Viabilidade
Feminino
Liofilização
Fidelidade a Diretrizes
Seres Humanos
Masculino
Plasma
Estudos Retrospectivos
Resultado do Tratamento
Ferimentos e Lesões/mortalidade
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170921
[St] Status:MEDLINE
[do] DOI:10.1097/TA.0000000000001569



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