Base de dados : MEDLINE
Pesquisa : E01.370.225.750 [Categoria DeCS]
Referências encontradas : 15548 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 1555 ir para página                         

  1 / 15548 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29193081
[Au] Autor:Zaidi MY; Canter R; Cardona K
[Ad] Endereço:Division of Surgical Oncology, Winship Cancer Institute, Emory University School of Medicine, Atlanta, Georgia.
[Ti] Título:Post-operative surveillance in retroperitoneal soft tissue sarcoma: The importance of tumor histology in guiding strategy.
[So] Source:J Surg Oncol;117(1):99-104, 2018 Jan.
[Is] ISSN:1096-9098
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Crafting effective surveillance strategies for retroperitoneal soft tissue sarcomas is difficult given the scarcity of literature on this rare disease. In this article, we will summarize the most recent literature on natural history and recurrence patterns of RPS compiled from multi-institutional collaborative studies and centers with extended follow-up data, and based on this evidence, propose principles for histology-based post-operative surveillance protocols.
[Mh] Termos MeSH primário: Técnicas Histológicas/métodos
Vigilância da População
Complicações Pós-Operatórias/diagnóstico
Neoplasias Retroperitoneais/patologia
Sarcoma/patologia
[Mh] Termos MeSH secundário: Terapia Combinada
Seres Humanos
Complicações Pós-Operatórias/epidemiologia
Neoplasias Retroperitoneais/terapia
Sarcoma/terapia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE
[do] DOI:10.1002/jso.24927


  2 / 15548 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29320521
[Au] Autor:Ziai J; Gilbert HN; Foreman O; Eastham-Anderson J; Chu F; Huseni M; Kim JM
[Ad] Endereço:Department of Pathology, Genentech, Inc., South San Francisco, California, United States of America.
[Ti] Título:CD8+ T cell infiltration in breast and colon cancer: A histologic and statistical analysis.
[So] Source:PLoS One;13(1):e0190158, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The prevalence of cytotoxic tumor infiltrating lymphocytes (TILs) has demonstrated prognostic value in multiple tumor types. In particular, CD8 counts (in combination with CD3 and CD45RO) have been shown to be superior to traditional UICC staging in colon cancer patients and higher total CD8 counts have been associated with better survival in breast cancer patients. However, immune infiltrate heterogeneity can lead to potentially significant misrepresentations of marker prevalence in routine histologic sections. We examined step sections of breast and colorectal cancer samples for CD8+ T cell prevalence by standard chromogenic immunohistochemistry to determine marker variability and inform practice of T cell biomarker assessment in formalin-fixed, paraffin-embedded (FFPE) tissue samples. Stained sections were digitally imaged and CD8+ lymphocytes within defined regions of interest (ROI) including the tumor and surrounding stroma were enumerated. Statistical analyses of CD8+ cell count variability using a linear model/ANOVA framework between patients as well as between levels within a patient sample were performed. Our results show that CD8+ T-cell distribution is highly homogeneous within a standard tissue sample in both colorectal and breast carcinomas. As such, cytotoxic T cell prevalence by immunohistochemistry on a single level or even from a subsample of biopsy fragments taken from that level can be considered representative of cytotoxic T cell infiltration for the entire tumor section within the block. These findings support the technical validity of biomarker strategies relying on CD8 immunohistochemistry.
[Mh] Termos MeSH primário: Neoplasias da Mama/imunologia
Linfócitos T CD8-Positivos/patologia
Carcinoma/imunologia
Neoplasias Colorretais/imunologia
Linfócitos do Interstício Tumoral/patologia
Subpopulações de Linfócitos T/patologia
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Biópsia/métodos
Neoplasias da Mama/patologia
Linfócitos T CD8-Positivos/imunologia
Carcinoma/patologia
Neoplasias Colorretais/patologia
Simulação por Computador
Citotoxicidade Imunológica
Feminino
Técnicas Histológicas
Seres Humanos
Processamento de Imagem Assistida por Computador
Contagem de Linfócitos
Linfócitos do Interstício Tumoral/imunologia
Masculino
Meia-Idade
Prognóstico
Curva ROC
Subpopulações de Linfócitos T/imunologia
Linfócitos T Citotóxicos/imunologia
Linfócitos T Citotóxicos/patologia
Microambiente Tumoral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190158


  3 / 15548 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28985569
[Au] Autor:Richardson DS; Lichtman JW
[Ad] Endereço:Harvard Center for Biological Imaging, Harvard University, Cambridge, MA 02138, USA; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
[Ti] Título:SnapShot: Tissue Clearing.
[So] Source:Cell;171(2):496-496.e1, 2017 Oct 05.
[Is] ISSN:1097-4172
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tissue clearing has become an important tool for the investigation of biological systems in three dimensions. However, many pioneering techniques were based on serendipitous discoveries. Next-generation clearing methods have been (re)designed with a better understanding of the chemistry and physics required to equalize the refractive index throughout a sample which prevents the random bending of light that clouds biological tissues.
[Mh] Termos MeSH primário: Técnicas Histológicas/métodos
Microscopia/métodos
[Mh] Termos MeSH secundário: Luz
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171007
[St] Status:MEDLINE


  4 / 15548 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28934283
[Au] Autor:Dimitropoulos K; Barmpoutis P; Zioga C; Kamas A; Patsiaoura K; Grammalidis N
[Ad] Endereço:Information Technologies Institute, Centre for Research and Technology Hellas, Thessaloniki, Greece.
[Ti] Título:Grading of invasive breast carcinoma through Grassmannian VLAD encoding.
[So] Source:PLoS One;12(9):e0185110, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this paper we address the problem of automated grading of invasive breast carcinoma through the encoding of histological images as VLAD (Vector of Locally Aggregated Descriptors) representations on the Grassmann manifold. The proposed method considers each image as a set of multidimensional spatially-evolving signals that can be efficiently modeled through a higher-order linear dynamical systems analysis. Subsequently, each H&E (Hematoxylin and Eosin) stained breast cancer histological image is represented as a cloud of points on the Grassmann manifold, while a vector representation approach is applied aiming to aggregate the Grassmannian points based on a locality criterion on the manifold. To evaluate the efficiency of the proposed methodology, two datasets with different characteristics were used. More specifically, we created a new medium-sized dataset consisting of 300 annotated images (collected from 21 patients) of grades 1, 2 and 3, while we also provide experimental results using a large dataset, namely BreaKHis, containing 7,909 breast cancer histological images, collected from 82 patients, of both benign and malignant cases. Experimental results have shown that the proposed method outperforms a number of state of the art approaches providing average classification rates of 95.8% and 91.38% with our dataset and the BreaKHis dataset, respectively.
[Mh] Termos MeSH primário: Neoplasias da Mama/classificação
Neoplasias da Mama/patologia
Técnicas Histológicas
Interpretação de Imagem Assistida por Computador/métodos
Gradação de Tumores/métodos
[Mh] Termos MeSH secundário: Algoritmos
Neoplasias da Mama/diagnóstico
Conjuntos de Dados como Assunto
Seres Humanos
Modelos Lineares
Invasividade Neoplásica/diagnóstico
Invasividade Neoplásica/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170922
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185110


  5 / 15548 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Texto completo
[PMID]:28731502
[Au] Autor:Novak G; Parker CE; Pai RK; MacDonald JK; Feagan BG; Sandborn WJ; D'Haens G; Jairath V; Khanna R
[Ad] Endereço:Department of Gastroenterology, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands.
[Ti] Título:Histologic scoring indices for evaluation of disease activity in Crohn's disease.
[So] Source:Cochrane Database Syst Rev;7:CD012351, 2017 Jul 21.
[Is] ISSN:1469-493X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Histologic assessment of mucosal disease activity has been increasingly used in clinical trials of treatment for Crohn's disease. However, the operating properties of the currently existing histologic scoring indices remain unclear. OBJECTIVES: A systematic review was undertaken to evaluate the development and operating characteristics of available histologic disease activity indices in Crohn's disease. SEARCH METHODS: Electronic searches of MEDLINE, EMBASE, PubMed, and the Cochrane Library (CENTRAL) databases from inception to 20 July 2016 were supplemented by manual reviews of bibliographies and abstracts submitted to major gastroenterology meetings (Digestive Disease Week, United European Gastroenterology Week, European Crohn's and Colitis Organisation). SELECTION CRITERIA: Any study design (e.g. randomised controlled trial, cohort study, case series) that evaluated a histologic disease activity index in patients with Crohn's disease was considered for inclusion. Study participants included adult patients (> 16 years), diagnosed with Crohn's disease using conventional clinical, radiographic or endoscopic criteria. DATA COLLECTION AND ANALYSIS: Two authors independently reviewed the titles and abstracts of the studies identified from the literature search. The full text of potentially relevant citations were reviewed for inclusion and the study investigators were contacted as needed for clarification. Any disagreements regarding study eligibility were resolved by discussion and consensus with a third author.Two authors independently extracted and recorded data using a standard form. The following data were recorded from each eligible study: number of patients enrolled; number of patients per treatment arm; patient characteristics: age and gender distribution; description of histologic disease activity index utilized; and outcomes such as content validity, construct validity, criterion validity, responsiveness, intra-rater reliability, inter-rater reliability, and feasibility. MAIN RESULTS: Sixteen reports of 14 studies describing 14 different numerical histological indices fulfilled the inclusion criteria.Inter-rater reliability was assessed in one study. For the Naini and Cortina Score, estimates of correlation were 'almost perfect', ranging from r = 0.94 to 0.96. The methodological quality of this study with respect to reliability was 'good'.With respect to validity, correlation estimates between various histological scoring systems and Crohn's disease activity as measured by objective markers of inflammation (including C-reactive protein, erythrocyte sedimentation rate, fecal calprotectin and fecal lactoferrin); endoscopic disease activity scores; clinical disease activity scores; and quality of life questionnaires were reported. Comparisons between histologic scoring indices and endoscopic scoring indices ranged from no correlation to 'substantial' (r = 0.779). The methodological quality of the studies that explored validity ranged form 'poor' to 'good'.Responsiveness data were available in seven studies. After subjects were administered a treatment of known efficacy, statistically significant change in the index score was demonstrated in five studies with respect to six indices. Two studies failed to indicate whether there was statistically significant change in the index score post-treatment. With regard to methodological quality, six of the studies were rated as 'poor' and one of the studies was rated as 'fair'.Feasibility was assessed by one study. The Naini and Cortina Score was shown to be simple to use and feasible for every given case. AUTHORS' CONCLUSIONS: Currently there is no fully validated histological scoring index for evaluation of Crohn's disease activity. Development of a validated histological scoring index for Crohn's disease is a clinical and research priority.
[Mh] Termos MeSH primário: Doença de Crohn/patologia
[Mh] Termos MeSH secundário: Adulto
Biópsia
Colo/patologia
Colonoscopia
Técnicas Histológicas/métodos
Técnicas Histológicas/normas
Seres Humanos
Íleo/patologia
Estudos Prospectivos
Reto/patologia
Reprodutibilidade dos Testes
Estudos Retrospectivos
[Pt] Tipo de publicação:JOURNAL ARTICLE; META-ANALYSIS; REVIEW
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170908
[Lr] Data última revisão:
170908
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170722
[St] Status:MEDLINE
[do] DOI:10.1002/14651858.CD012351.pub2


  6 / 15548 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28714966
[Au] Autor:Zhao Y; Bucur O; Irshad H; Chen F; Weins A; Stancu AL; Oh EY; DiStasio M; Torous V; Glass B; Stillman IE; Schnitt SJ; Beck AH; Boyden ES
[Ad] Endereço:MIT Media Lab, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
[Ti] Título:Nanoscale imaging of clinical specimens using pathology-optimized expansion microscopy.
[So] Source:Nat Biotechnol;35(8):757-764, 2017 Aug.
[Is] ISSN:1546-1696
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Expansion microscopy (ExM), a method for improving the resolution of light microscopy by physically expanding a specimen, has not been applied to clinical tissue samples. Here we report a clinically optimized form of ExM that supports nanoscale imaging of human tissue specimens that have been fixed with formalin, embedded in paraffin, stained with hematoxylin and eosin, and/or fresh frozen. The method, which we call expansion pathology (ExPath), converts clinical samples into an ExM-compatible state, then applies an ExM protocol with protein anchoring and mechanical homogenization steps optimized for clinical samples. ExPath enables ∼70-nm-resolution imaging of diverse biomolecules in intact tissues using conventional diffraction-limited microscopes and standard antibody and fluorescent DNA in situ hybridization reagents. We use ExPath for optical diagnosis of kidney minimal-change disease, a process that previously required electron microscopy, and we demonstrate high-fidelity computational discrimination between early breast neoplastic lesions for which pathologists often disagree in classification. ExPath may enable the routine use of nanoscale imaging in pathology and clinical research.
[Mh] Termos MeSH primário: Processamento de Imagem Assistida por Computador/métodos
Microscopia/métodos
Imagem Molecular/métodos
Nanomedicina/métodos
[Mh] Termos MeSH secundário: Biópsia
Mama/diagnóstico por imagem
Mama/patologia
Mama/ultraestrutura
Feminino
Técnicas Histológicas
Seres Humanos
Rim/diagnóstico por imagem
Rim/patologia
Rim/ultraestrutura
Nefrose Lipoide/diagnóstico por imagem
Nefrose Lipoide/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170924
[Lr] Data última revisão:
170924
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170718
[St] Status:MEDLINE
[do] DOI:10.1038/nbt.3892


  7 / 15548 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28601012
[Au] Autor:Näslund J; Fick J; Asker N; Ekman E; Larsson DGJ; Norrgren L
[Ad] Endereço:Section of Pathology, Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden. Electronic address: johanna.naslund@slu.se.
[Ti] Título:Diclofenac affects kidney histology in the three-spined stickleback (Gasterosteus aculeatus) at low µg/L concentrations.
[So] Source:Aquat Toxicol;189:87-96, 2017 Aug.
[Is] ISSN:1879-1514
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Diclofenac, a commonly used non-steroidal anti-inflammatory drug, is considered for regulation under the European water framework directive. This is because effects on fish have been reported at concentrations around those regularly found in treated sewage effluents (∼1µg/L). However, a recent publication reports no effects on fish at 320µg/L. In this study, three-spined sticklebacks (Gasterosteus aculeatus) were exposed to 0, 4.6, 22, 82 and 271µg/L diclofenac in flow-through systems for 28days using triplicate aquaria per concentration. At the highest concentration, significant mortalities were observed already after 21days (no mortalities found up to 22µg/L). Histological analysis revealed a significant increase in the proportion of renal hematopoietic tissue (renal hematopoietic hyperplasia) after 28days at the lowest concentration and at all higher concentrations, following a clear dose-response pattern. Skin ulcerations of the jaw were noted by macroscopic observations, primarily at the two highest concentrations. No histological changes were observed in the liver. There was an increase in the relative hepatic mRNA levels of c7 (complement component 7), a gene involved in the innate immune system, at 22µg/L and at all higher concentrations, again following a clear dose-response. The bioconcentration factor was stable across concentrations, but lower than reported for rainbow trout, suggesting lower internal exposure to the drug in the stickleback. In conclusion, this study demonstrates that diclofenac causes histological changes in the three-spined stickleback at low µg/L concentrations, which cause concern for fish populations exposed to treated sewage effluents.
[Mh] Termos MeSH primário: Anti-Inflamatórios não Esteroides/toxicidade
Diclofenaco/toxicidade
Rim
Smegmamorpha/metabolismo
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios não Esteroides/metabolismo
Diclofenaco/metabolismo
Relação Dose-Resposta a Droga
Técnicas Histológicas
Rim/efeitos dos fármacos
Rim/patologia
Fígado/efeitos dos fármacos
Fígado/patologia
Oncorhynchus mykiss/metabolismo
RNA Mensageiro/genética
Smegmamorpha/genética
Poluentes Químicos da Água/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (RNA, Messenger); 0 (Water Pollutants, Chemical); 144O8QL0L1 (Diclofenac)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170611
[St] Status:MEDLINE


  8 / 15548 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28504673
[Au] Autor:Damisah EC; Hill RA; Tong L; Murray KN; Grutzendler J
[Ad] Endereço:Department of Neurology, Yale School of Medicine, New Haven, Connecticut, USA.
[Ti] Título:A fluoro-Nissl dye identifies pericytes as distinct vascular mural cells during in vivo brain imaging.
[So] Source:Nat Neurosci;20(7):1023-1032, 2017 Jul.
[Is] ISSN:1546-1726
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pericytes and smooth muscle cells are integral components of the brain microvasculature. However, no techniques exist to unambiguously identify these cell types, greatly limiting their investigation in vivo. Here we show that the fluorescent Nissl dye NeuroTrace 500/525 labels brain pericytes with specificity, allowing high-resolution optical imaging in the live mouse. We demonstrate that capillary pericytes are a population of mural cells with distinct morphological, molecular and functional features that do not overlap with precapillary or arteriolar smooth muscle actin-expressing cells. The remarkable specificity for dye uptake suggests that pericytes have molecular transport mechanisms not present in other brain cells. We demonstrate feasibility of longitudinal pericyte imaging during microvascular development and aging and in models of brain ischemia and Alzheimer's disease. The ability to easily label pericytes in any mouse model opens the possibility of a broad range of investigations of mural cells in vascular development, neurovascular coupling and neuropathology.
[Mh] Termos MeSH primário: Técnicas Histológicas/métodos
Imagem Óptica/métodos
Pericitos/citologia
[Mh] Termos MeSH secundário: Envelhecimento/metabolismo
Doença de Alzheimer/metabolismo
Animais
Encéfalo/irrigação sanguínea
Isquemia Encefálica/metabolismo
Feminino
Corantes Fluorescentes/metabolismo
Masculino
Camundongos
Camundongos Transgênicos
Miócitos de Músculo Liso/citologia
Pericitos/metabolismo
Pericitos/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fluorescent Dyes)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171115
[Lr] Data última revisão:
171115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170516
[St] Status:MEDLINE
[do] DOI:10.1038/nn.4564


  9 / 15548 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28414331
[Au] Autor:Tay TL; Mai D; Dautzenberg J; Fernández-Klett F; Lin G; Sagar; Datta M; Drougard A; Stempfl T; Ardura-Fabregat A; Staszewski O; Margineanu A; Sporbert A; Steinmetz LM; Pospisilik JA; Jung S; Priller J; Grün D; Ronneberger O; Prinz M
[Ad] Endereço:Institute of Neuropathology, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
[Ti] Título:A new fate mapping system reveals context-dependent random or clonal expansion of microglia.
[So] Source:Nat Neurosci;20(6):793-803, 2017 Jun.
[Is] ISSN:1546-1726
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Microglia constitute a highly specialized network of tissue-resident immune cells that is important for the control of tissue homeostasis and the resolution of diseases of the CNS. Little is known about how their spatial distribution is established and maintained in vivo. Here we establish a new multicolor fluorescence fate mapping system to monitor microglial dynamics during steady state and disease. Our findings suggest that microglia establish a dense network with regional differences, and the high regional turnover rates found challenge the universal concept of microglial longevity. Microglial self-renewal under steady state conditions constitutes a stochastic process. During pathology this randomness shifts to selected clonal microglial expansion. In the resolution phase, excess disease-associated microglia are removed by a dual mechanism of cell egress and apoptosis to re-establish the stable microglial network. This study unravels the dynamic yet discrete self-organization of mature microglia in the healthy and diseased CNS.
[Mh] Termos MeSH primário: Linhagem da Célula/fisiologia
Técnicas Histológicas/métodos
Microglia/citologia
[Mh] Termos MeSH secundário: Animais
Apoptose/fisiologia
Encéfalo/citologia
Receptor 1 de Quimiocina CX3C
Contagem de Células/métodos
Proliferação Celular/fisiologia
Feminino
Homeostase/fisiologia
Camundongos
Camundongos Transgênicos
Microglia/fisiologia
Modelos Biológicos
Degeneração Neural/fisiopatologia
Receptores de Quimiocinas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CX3C Chemokine Receptor 1); 0 (Cx3cr1 protein, mouse); 0 (Receptors, Chemokine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170418
[St] Status:MEDLINE
[do] DOI:10.1038/nn.4547


  10 / 15548 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28328876
[Au] Autor:Ward JM; Schofield PN; Sundberg JP
[Ad] Endereço:Global VetPathology, Montgomery Village, Maryland, USA.
[Ti] Título:Reproducibility of histopathological findings in experimental pathology of the mouse: a sorry tail.
[So] Source:Lab Anim (NY);46(4):146-151, 2017 Mar 22.
[Is] ISSN:1548-4475
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Reproducibility of in vivo research using the mouse as a model organism depends on many factors, including experimental design, strain or stock, experimental protocols, and methods of data evaluation. Gross and histopathology are often the endpoints of such research and there is increasing concern about the accuracy and reproducibility of diagnoses in the literature. To reproduce histopathological results, the pathology protocol, including necropsy methods and slide preparation, should be followed by interpretation of the slides by a pathologist familiar with reading mouse slides and familiar with the consensus medical nomenclature used in mouse pathology. Likewise, it is important that pathologists are consulted as reviewers of manuscripts where histopathology is a key part of the investigation. The absence of pathology expertise in planning, executing and reviewing in vivo research using mice leads to questionable pathology-based findings and conclusions from studies, even in high-impact journals. We discuss the various aspects of this problem, give some examples from the literature and suggest solutions.
[Mh] Termos MeSH primário: Camundongos
Patologia Veterinária/métodos
[Mh] Termos MeSH secundário: Animais
Competência Clínica
Técnicas Histológicas/métodos
Neoplasias/diagnóstico
Neoplasias/patologia
Neoplasias/veterinária
Patologia Veterinária/normas
Reprodutibilidade dos Testes
Terminologia como Assunto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170714
[Lr] Data última revisão:
170714
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170323
[St] Status:MEDLINE
[do] DOI:10.1038/laban.1214



página 1 de 1555 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde