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[PMID]:28246608
[Au] Autor:Liu H; Zhu R; Liu C; Ma R; Wang L; Chen B; Li L; Niu J; Zhao D; Mo F; Fu M; Brömme D; Zhang D; Gao S
[Ad] Endereço:Preclinical Medicine School, Beijing University of Chinese Medicine, Beijing 100029, China.
[Ti] Título:Evaluation of Decalcification Techniques for Rat Femurs Using HE and Immunohistochemical Staining.
[So] Source:Biomed Res Int;2017:9050754, 2017.
[Is] ISSN:2314-6141
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:. In routine histopathology, decalcification is an essential step for mineralized tissues. The purpose of this study is to evaluate the effects of different decalcification solutions on the morphological and antigenicity preservation in Sprague Dawley (SD) rat femurs. . Four different decalcification solutions were employed to remove the mineral substances from rat femurs, including 10% neutral buffered EDTA, 3% nitric acid, 5% nitric acid, and 8% hydrochloric acid/formic acid. Shaking and low temperature were used to process the samples. The stainings of hematoxylin-eosin (HE) and immunohistochemical (IHC) were employed to evaluate the bone morphology and antigenicity. . Different decalcification solutions may affect the quality of morphology and the staining of paraffin-embedded sections in pathological examinations. Among four decalcifying solutions, 3% nitric acid is the best decalcifying agent for HE staining. 10% neutral buffered EDTA and 5% nitric acid are the preferred decalcifying agents for IHC staining. . The current study investigated the effects of different decalcifying agents on the preservation of the bone structure and antigenicity, which will help to develop suitable protocols for the analyses of the bony tissue.
[Mh] Termos MeSH primário: Técnica de Descalcificação/métodos
Amarelo de Eosina-(YS)/metabolismo
Fêmur/patologia
Hematoxilina/metabolismo
Imuno-Histoquímica/métodos
Coloração e Rotulagem
[Mh] Termos MeSH secundário: Animais
Antígenos/metabolismo
Feminino
Processamento de Imagem Assistida por Computador
Ratos Sprague-Dawley
Soluções
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens); 0 (Solutions); TDQ283MPCW (Eosine Yellowish-(YS)); YKM8PY2Z55 (Hematoxylin)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170313
[Lr] Data última revisão:
170313
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170302
[St] Status:MEDLINE
[do] DOI:10.1155/2017/9050754


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[PMID]:27720733
[Au] Autor:Righi S; Pileri S; Agostinelli C; Bacci F; Spagnolo S; Sabattini E
[Ad] Endereço:Haemolymphopathology Unit, Department of Haematology and Oncology "L & A Seragnoli", S. Orsola-Malpighi Hospital, University of Bologna, 40138 Bologna, Italy. Electronic address: simona.righi5@unibo.it.
[Ti] Título:Reproducibility of SOX-11 detection in decalcified bone marrow tissue in mantle cell lymphoma patients.
[So] Source:Hum Pathol;59:94-101, 2017 Jan.
[Is] ISSN:1532-8392
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mantle cell lymphoma (MCL) usually harbors the t(11;14)(q13;q32) with overexpression of CCND1 mRNA and transcription of the cyclin D1 nuclear protein. Regardless of CCND1 status, most MCLs also express the SOX11 nuclear protein, which is thus helpful in the diagnosis of the rare CCND1-negative MCLs. Recently, SOX11 has been reported to be often negative in MCLs clinically resembling marginal zone lymphoma and recently defined as "leukemic non-nodal" MCL in the incoming revision of the WHO classification of lymphoid tumors, for which the bone marrow biopsy is commonly the first diagnostic approach. Due to the less aggressive clinical behavior of the latter MCLs, the reliable determination of the SOX11 antigen in decalcified tissue is mandatory. To this end, since little data are available in the literature, four commercially available anti-SOX11 antibodies (two polyclonal and two monoclonal) were tested on 21 positive staging bone marrow (BM) biopsies from cyclin D1/SOX11-positive MCL patients (17 fixed in B5, 4 in 10% buffered formalin) and on 9 positive BM biopsies from leukemic non-nodal MCL patients. The results were compared for specificity, sensitivity, staining strength and degree of an additional staining on myeloid precursors, also evaluating possible impact of the different fixatives used. Non-mantle cell lymphomas were also tested to address specificity. All reagents showed high sensitivity but the monoclonal code CMC38221001 provided the highest specificity and the lowest degree of non-lymphoid staining on myeloid cells. Formalin fixation generally improved the performance of most antibodies when compared to B5 fixation.
[Mh] Termos MeSH primário: Biomarcadores Tumorais/análise
Exame de Medula Óssea/métodos
Medula Óssea/química
Técnica de Descalcificação
Imuno-Histoquímica
Linfoma de Célula do Manto/química
Fatores de Transcrição SOXC/análise
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Anticorpos Monoclonais/imunologia
Especificidade de Anticorpos
Biomarcadores Tumorais/imunologia
Biópsia
Medula Óssea/imunologia
Medula Óssea/patologia
Feminino
Fixadores
Formaldeído
Seres Humanos
Linfoma de Célula do Manto/imunologia
Linfoma de Célula do Manto/patologia
Masculino
Meia-Idade
Valor Preditivo dos Testes
Reprodutibilidade dos Testes
Fatores de Transcrição SOXC/imunologia
Fixação de Tecidos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Biomarkers, Tumor); 0 (Fixatives); 0 (SOX11 protein, human); 0 (SOXC Transcription Factors); 1HG84L3525 (Formaldehyde)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161011
[St] Status:MEDLINE


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[PMID]:26950817
[Au] Autor:Urs AB; Singh H; Rawat G; Mohanty S; Ghosh S
[Ti] Título:Cementoblastoma Solely Involving Maxillary Primary Teeth--A Rare Presentation.
[So] Source:J Clin Pediatr Dent;40(2):147-51, 2016.
[Is] ISSN:1053-4628
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cementoblastoma is a rare benign neoplasm of odontogenic ectomesenchyme origin, involving the roots of any tooth, which occurs predominantly in second and third decade of life. Very few cases of cementoblastoma associated with a primary tooth or having a maxillary presentation have been reported in the past. Here, a rare case of a ten year old boy who presented to the department with a swelling in maxillary posterior region since one month is being discussed. The radiographic presentation was mimicking an odontoma. The final diagnosis was cementoblastoma. We have advocated the use of polarized microscopy to support the histopathological diagnosis with respect to its cemental origin. Cementoblastoma should be considered in the differential diagnosis of radio-opaque lesions in the transitional dentition.
[Mh] Termos MeSH primário: Neoplasias Maxilares/diagnóstico
Dente Molar/patologia
Tumores Odontogênicos/diagnóstico
Dente Decíduo/patologia
[Mh] Termos MeSH secundário: Criança
Técnica de Descalcificação
Cemento Dentário/patologia
Seres Humanos
Masculino
Neoplasias Maxilares/patologia
Tumores Odontogênicos/patologia
Radiografia Panorâmica
Ápice Dentário/patologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1605
[Cu] Atualização por classe:160308
[Lr] Data última revisão:
160308
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:160308
[St] Status:MEDLINE
[do] DOI:10.17796/1053-4628-40.2.147


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[PMID]:26358636
[Au] Autor:Damante CA; Ducati P; Ferreira R; Salmeron S; Zangrando MS; de Rezende ML; Sant'Ana AC; Greghi SL; Magalhães AC
[Ad] Endereço:Discipline of Periodontics, Bauru School of Dentistry, Brazil. Electronic address: cdamante@usp.br.
[Ti] Título:In vitro evaluation of adhesion/proliferation of human gingival fibroblasts on demineralized root surfaces by toluidine blue O in antimicrobial photodynamic therapy.
[So] Source:Photodiagnosis Photodyn Ther;13:303-7, 2016 Mar.
[Is] ISSN:1873-1597
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Antimicrobial photodynamic therapy (aPDT) in Dentistry has important effects as bacterial destruction in areas with periodontal disease. Some dyes applied in aPDT could present low pH and, consequently, result in tooth demineralization. This study evaluated demineralization produced by aPDT with toluidine blue O (TBO) at low pH and analyzed adhesion/proliferation of human gingival fibroblasts (HGF). METHODS: In the 1st phase, bovine enamel and root dentin fragments received 2 treatments: PDT4 group (TBO-100 µg/ml-pH 4-60s) plus laser (660 nm, 45 J/cm(2), 1.08 J, 30 mW, 30 s, spot 0.024 cm(2), 1.25 W/cm(2), sweeping, non-contact) and CA group (citric acid plus tetracycline-pH 1-180 s). Surface hardness loss and tooth wear were statistically analyzed (Student's t test, ANOVA/Tukey, p<0.05). In the 2nd phase, human dentin fragments were divided in C (control group-scaling and root planing), PDT4 and CA. HGF (10(4), 5th passage) were cultured on these fragments for 24, 48 and 72 h and counted in scanning electron microscopy photographs. Number of HGF was analyzed using repeated-measures ANOVA and Tukey (p<0.05). RESULTS: Percentage of surface hardness loss was similar in dentin for PDT4 (71.5%) and CA (76.1%) (p>0.05) and higher in enamel for CA (68.0%) compared to PDT4 (34.1%) (p<0.05). In respect to wear, no difference was found between PDT4 (dentin: 12.58 µm, enamel: 12.19 µm respectively) and CA (dentin: 11.74 µm and enamel: 11.03 µm) (p>0.05). Number of HGF was higher after 72 h in CA group (2.66, p<0.05) compared to PDT4 (2.2) and C (1.33). CONCLUSION: PDT4 is not as aggressive as CA for enamel. However, dentin demineralized promoted by PDT4 does not stimulate HGF adhesion and proliferation as CA.
[Mh] Termos MeSH primário: Desmineralização Patológica Óssea/induzido quimicamente
Desmineralização Patológica Óssea/patologia
Esmalte Dentário/efeitos dos fármacos
Fibroblastos/efeitos dos fármacos
Fotoquimioterapia/métodos
Cloreto de Tolônio/efeitos adversos
Raiz Dentária/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Infecções Bacterianas/tratamento farmacológico
Infecções Bacterianas/patologia
Bovinos
Adesão Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Técnica de Descalcificação
Esmalte Dentário/patologia
Fibroblastos/patologia
Gengiva/efeitos dos fármacos
Gengiva/patologia
Gengivite/tratamento farmacológico
Gengivite/patologia
Técnicas In Vitro
Fármacos Fotossensibilizantes/administração & dosagem
Fármacos Fotossensibilizantes/efeitos adversos
Cloreto de Tolônio/administração & dosagem
Raiz Dentária/patologia
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Photosensitizing Agents); 15XUH0X66N (Tolonium Chloride)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150912
[St] Status:MEDLINE


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[PMID]:26057927
[Au] Autor:Rehman K; Khan FR; Habib S
[Ad] Endereço:Assistant Professor, Department of Operative Dentistry, Liaquat National Hospital and Medical College, Karachi 74800, Pakistan, Phone: +92 3212672957, e-mail: dr.k.rehman@gmail.com.
[Ti] Título:Diaphonization: a recipe to study teeth.
[So] Source:J Contemp Dent Pract;16(3):248-51, 2015 03 01.
[Is] ISSN:1526-3711
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:AIM: There are various techniques to study root canal morphology and diaphonization is one of them. There are various methods of decalcification and diaphonization, cited in literature and the main aim of this paper was to give a brief account of the various techniques and share our experience of the technique at a teaching institution in Karachi, Pakistan. MATERIALS AND METHODS: Diaphonization is one of the oldest methods and is based on decalcification of teeth followed by clearing and dye penetration. The specimen is later studied under microscope without sectioning. RESULTS: After the process of clearing a three-dimensional (3D) structure of the internal canal anatomy was visible with naked eye. CONCLUSION: This paper entails a detailed historical background as well as the author's technique including percentages of various chemicals used and the timing of immersion of teeth into these agents. CLINICAL SIGNIFICANCE: The read out is simple and can be subjected to interpretation by direct observation under microscope and can be helpful for students undertaking research in not only the discipline of dentistry but also in other fields such as botany and zoology.
[Mh] Termos MeSH primário: Corantes
Técnica de Descalcificação/métodos
Cavidade Pulpar/anatomia & histologia
[Mh] Termos MeSH secundário: 2-Propanol/química
Fixadores/química
Seres Humanos
Ácido Nítrico/química
Salicilatos/química
Fatores de Tempo
Fixação de Tecidos/métodos
Xilenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coloring Agents); 0 (Fixatives); 0 (Salicylates); 0 (Xylenes); 411VRN1TV4 (Nitric Acid); LAV5U5022Y (methyl salicylate); ND2M416302 (2-Propanol)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170508
[Lr] Data última revisão:
170508
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:150610
[St] Status:MEDLINE


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[PMID]:25977137
[Au] Autor:Torlakovic EE; Brynes RK; Hyjek E; Lee SH; Kreipe H; Kremer M; McKenna R; Sadahira Y; Tzankov A; Reis M; Porwit A; International Council for Standardization in Haematology
[Ad] Endereço:Department of Laboratory Hematology, University Health Network, University of Toronto, Toronto, ON, Canada.
[Ti] Título:ICSH guidelines for the standardization of bone marrow immunohistochemistry.
[So] Source:Int J Lab Hematol;37(4):431-49, 2015 Aug.
[Is] ISSN:1751-553X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bone marrow (BM) tissue biopsy evaluation, including trephine biopsy and clot section, is an integral part of BM investigation and is often followed by ancillary studies, in particular immunohistochemistry (IHC). IHC provides in situ coupling of morphological assessment and immunophenotype. The number of different IHC tests that can be applied to BM trephine biopsies and the number of indications for IHC testing is increasing concurrently with the development of flow cytometry and molecular diagnostic methods. An international Working Party for the Standardization of Bone Marrow IHC was formed by the International Council for Standardization in Hematology (ICSH) to prepare a set of guidelines for the standardization of BM IHC based on currently available published evidence and modern understanding of quality assurance principles as applied to IHC in general. The guidelines were discussed at the ICSH General Assemblies and reviewed by an international panel of experts to achieve further consensus and represent further development of the previously published ICSH guidelines for the standardization of BM specimens handling and reports.
[Mh] Termos MeSH primário: Exame de Medula Óssea/normas
Medula Óssea/patologia
Citometria de Fluxo/normas
Imuno-Histoquímica/normas
Imunofenotipagem/normas
[Mh] Termos MeSH secundário: Biópsia/normas
Medula Óssea/cirurgia
Técnica de Descalcificação/normas
Seres Humanos
Cooperação Internacional
Ensaio de Proficiência Laboratorial
Inclusão em Parafina/normas
Controle de Qualidade
Fixação de Tecidos/normas
[Pt] Tipo de publicação:GUIDELINE; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1604
[Cu] Atualização por classe:150716
[Lr] Data última revisão:
150716
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150516
[St] Status:MEDLINE
[do] DOI:10.1111/ijlh.12365


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[PMID]:25636435
[Au] Autor:Visinoni F
[Ad] Endereço:Milestone Srl, Via Fatebenefratelli 1/5, 24010, Sorisole Bergamo, Italy, f.visinoni@milestonemedsrl.com.
[Ti] Título:Towards the lean lab: the industry challenge.
[So] Source:Recent Results Cancer Res;199:119-33, 2015.
[Is] ISSN:0080-0015
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In anatomic pathology, the current state encompassing the pre-analytic processes of tissue collection, handling, examination, preparation, processing, and storage are largely uncontrolled, inconsistently performed, and/or not standardized according to the sound scientific data. Pre-analytic defects result in nearly three-quarters of the problems in laboratory diagnostics. This is evident in quality surveys from well-respected institutions that document high miss rates in the required basics of information related to patient and tissue identity, let alone parameters documenting quality aspects related to the surgical specimen and its preservation. This talk will describe the historical approach to tissue processing and identify gaps from worldwide observations in current laboratory practices. It will also offer potential methodological and technological solutions and process improvements that laboratories may consider in serving the ultimate users of pathology information: the clinician and the patient. It illustrates the need for scientifically validated specimen guidelines and a performance based, standardized and documented "chain of custody" of the pre-analytical steps from the patient's body through fixation. For thought leaders and professional standard setters, opportunities for optimizing molecular studies exist in specimen collection, transfer, grossing, fixation, and decalcification protocols. In this evolving era of molecular profiling and personalized therapeutic decision-making, a well-reasoned and coordinated focus on pre-analytic processes that optimizes specimens for subsequent testing will result in: Improved specimen quality for molecular testing Improved accuracy of diagnostic and molecular test results Reduced Turnaroundtimes for same-day diagnosis Enhanced satisfaction of clinicians and patients.
[Mh] Termos MeSH primário: Serviços de Laboratório Clínico/tendências
Padrões de Prática Médica/tendências
Manejo de Espécimes
[Mh] Termos MeSH secundário: Serviços de Laboratório Clínico/normas
Técnica de Descalcificação/instrumentação
Técnica de Descalcificação/métodos
Seres Humanos
Laboratórios/tendências
Microtomia/instrumentação
Microtomia/normas
Microtomia/tendências
Padrões de Prática Médica/normas
Manejo de Espécimes/instrumentação
Manejo de Espécimes/normas
Manejo de Espécimes/tendências
Preservação de Tecido/instrumentação
Preservação de Tecido/normas
Preservação de Tecido/tendências
Coleta de Tecidos e Órgãos/instrumentação
Coleta de Tecidos e Órgãos/normas
Coleta de Tecidos e Órgãos/tendências
Transportes/instrumentação
Transportes/normas
Vácuo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1506
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150201
[St] Status:MEDLINE
[do] DOI:10.1007/978-3-319-13957-9_12


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[PMID]:25630848
[Au] Autor:Mayer A; Royer MC; Summerlin DJ; Moore M; Galer C; Shipchandler TZ; Kokoska MS
[Ad] Endereço:Department of Otolaryngology-Head and Neck Surgery, Indiana University School of Medicine, Indianapolis, IN. Electronic address: almayer@iupui.edu.
[Ti] Título:Rapid mandible margins for intraoperative assessment.
[So] Source:Am J Otolaryngol;36(3):324-9, 2015 May-Jun.
[Is] ISSN:1532-818X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: To determine the feasibility of a rapid method of processing mandible bone margins for intraoperative histopathologic examination and to assess the relative value of fine, coarse, and core specimens in assessing bone margins. STUDY DESIGN: Prospective histologic controlled study. SETTING: A tertiary level academic medical center histopathology laboratory. SUBJECTS AND METHODS: Multiple bone samples were collected from fresh (<12 hours post-mortem) human cadaveric mandible using a 1) standard 4mm otolaryngologic cutting drill bit 2) diamond drill bit and 3) cutting core biopsy trocar. The specimens were placed in one of three decalcifying solutions (Decal A, Calex, EDTA Decal) from 15 to 75 minutes or control (fixation in 10% formalin). After each designated decalcification time period, specimens were cryosectioned or paraffin embedded and subsequently reviewed by a head and neck surgical pathologist. The specimens were assessed for overall quality, adequacy of decalcification, soft tissue quality, marrow quality, and presence of artifact. RESULTS: Bone margin specimens collected with a 4mm burr and processed with EDTA Decal for 30 minutes yielded the highest quality histopathologic slides compared to the other methods in a similar time frame. The adequacy of decalcification directly impacted the quality of histopathologic assessment. CONCLUSIONS: Mandible bone margins can be rapidly and safely prepared and adequately evaluated with only 30 minutes of decalcification. This method may provide acceptable intraoperative assessment of bone margins in patients with tumors which involve or approximate bone. We plan to examine this model in a prospective clinical study of patients with cancer invading mandibular bone.
[Mh] Termos MeSH primário: Técnica de Descalcificação/métodos
Técnicas de Preparação Histocitológica/métodos
Cuidados Intraoperatórios
Mandíbula/patologia
Mandíbula/cirurgia
[Mh] Termos MeSH secundário: Cadáver
Quelantes de Cálcio
Ácido Edético
Estudos de Viabilidade
Seres Humanos
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Calcium Chelating Agents); 9G34HU7RV0 (Edetic Acid)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150505
[Lr] Data última revisão:
150505
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150130
[St] Status:MEDLINE


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[PMID]:25611237
[Au] Autor:Dinges HC; Capper D; Ritz O; Brüderlein S; Marienfeld R; von Deimling A; Möller P; Lennerz JK
[Ad] Endereço:*Division of Molecular Diagnostics, Institute of Pathology, University Ulm, Ulm †Department of Neuropathology and Clinical Cooperation Unit Neuropathology, German Cancer Research Center (DKFZ), Institute of Pathology, Ruprecht-Karls-Universität Heidelberg, Heidelberg, Germany.
[Ti] Título:Validation of a Manual Protocol for BRAF V600E Mutation-specific Immunohistochemistry.
[So] Source:Appl Immunohistochem Mol Morphol;23(5):382-8, 2015 May-Jun.
[Is] ISSN:1533-4058
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Detection of BRAF V600E has diagnostic, prognostic, and therapeutic relevance. The recently developed BRAF V600E mutation-specific antibody has evolved into a feasible alternative to DNA analysis. The plethora of immunohistochemical protocols makes implementation tedious and, here we tested a set of manual and automated protocols and compared test performance with sequencing results. For assays, we employed formalin-fixed, in part decalcified, and paraffin-embedded tissue samples. Empiric testing of manual protocols included 10 variables in 17 protocols. Automated immunohistochemical staining and BRAF pyrosequencing served as independent test methods. Test performance measures were compared without considering 1 method as a standard. Four well-fixed samples (2WT/2Mut) were used for testing of all protocols and indicated 2 correctly classifying procedures. Practical performance assessment employed 33 independent tissue samples, composed of 27 leukemias (by pyrosequencing: 8 wild-type; 18 mutated; 1 noninformative) and 6 melanomas (V600E; V600K; wild-type, 2 each). Manual V600E staining was positive in 20 cases (19 of 20 V600E-containing samples plus the 1 sample that was noninformative), whereas all wild-type and V600K cases were immunonegative. Manual or automated staining as well as pyrosequencing would have missed an equal number of V600E-mutated cases and the correlation coefficient for these methods was 0.75 to 0.93 (substantial to almost perfect); the Youden index was 0.95. Detection of V600E-mutated BRAF at the protein level in routine and decalcified tissue samples is possible, and the presented manual protocols should expedite implementation in routine diagnostic practice. Our results indicate that both molecular techniques should be considered complementary.
[Mh] Termos MeSH primário: Biomarcadores Tumorais/genética
Imuno-Histoquímica/normas
Leucemia de Células Pilosas/diagnóstico
Melanoma/diagnóstico
Mutação
Proteínas Proto-Oncogênicas B-raf/genética
[Mh] Termos MeSH secundário: Ácido Aspártico/metabolismo
Automação Laboratorial
Análise Mutacional de DNA
Técnica de Descalcificação
Formaldeído
Expressão Gênica
Seres Humanos
Leucemia de Células Pilosas/genética
Leucemia de Células Pilosas/patologia
Melanoma/genética
Melanoma/patologia
Inclusão do Tecido
Fixação de Tecidos
Valina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 1HG84L3525 (Formaldehyde); 30KYC7MIAI (Aspartic Acid); EC 2.7.11.1 (BRAF protein, human); EC 2.7.11.1 (Proto-Oncogene Proteins B-raf); HG18B9YRS7 (Valine)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:161020
[Lr] Data última revisão:
161020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150123
[St] Status:MEDLINE
[do] DOI:10.1097/PAI.0000000000000092


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[PMID]:25458523
[Au] Autor:Chen M; Zhang Y; Dusevich V; Liu Y; Yu Q; Wang Y
[Ad] Endereço:Department of Prosthodontics, Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 401147, China; Department of Oral and Craniofacial Sciences, School of Dentistry, University of Missouri-Kansas City, Kansas City, MO 64108, USA.
[Ti] Título:Non-thermal atmospheric plasma brush induces HEMA grafting onto dentin collagen.
[So] Source:Dent Mater;30(12):1369-77, 2014 Dec.
[Is] ISSN:1879-0097
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Non-thermal atmospheric plasma (NTAP) brush has been regarded as a promising technique to enhance dental interfacial bonding. However, the principal enhancement mechanisms have not been well identified. In this study, the effect of non-thermal plasmas on grafting of HEMA, a typical dental monomer, onto dentin collagen thin films was investigated. METHODS: Human dentin was sectioned into 10-µm-thick films. After total demineralization in 0.5M EDTA solution for 30min, the dentin collagen films were water-rinsed, air-dried, treated with 35wt% HEMA aqueous solution. The films were then subject to plasma-exposure under a NTAP brush with different time (1-8min)/input power (5-15W). For comparison, the dentin collagen films were also treated with the above HEMA solution containing photo-initiators, then subject to light-curing. After plasma-exposure or light-curing, the HEMA-collagen films were rinsed in deionized water, and then examined by FTIR spectroscopy and TEM. RESULTS: The FITR results indicated that plasma-exposure could induce significant HEMA grafting onto dentin collagen thin films. In contrast, light-curing led to no detectable interaction of HEMA with dentin collagen. Quantitative IR spectral analysis (i.e., 1720/3075 or 749/3075, HEMA/collagen ratios) further suggested that the grafting efficacy of HEMA onto the plasma-exposed collagen thin films strongly depended on the treatment time and input power of plasmas. TEM results indicated that plasma treatment did not alter collagen's banding structure. SIGNIFICANCE: The current study provides deeper insight into the mechanism of dental adhesion enhancement induced by non-thermal plasmas treatment. The NTAP brush could be a promising method to create chemical bond between resin monomers and dentin collagen.
[Mh] Termos MeSH primário: Colágeno/química
Colagem Dentária/métodos
Dentina/química
Metacrilatos/química
Gases em Plasma/química
[Mh] Termos MeSH secundário: Compostos de Bifenilo/química
Cânfora/análogos & derivados
Cânfora/química
Técnica de Descalcificação
Ácido Edético/química
Seres Humanos
Cura Luminosa de Adesivos Dentários/métodos
Teste de Materiais
Microscopia Eletrônica de Transmissão
Oniocompostos/química
Fotoiniciadores Dentários/química
Espectrofotometria Infravermelho
Espectroscopia de Infravermelho com Transformada de Fourier
Propriedades de Superfície
Fatores de Tempo
para-Aminobenzoatos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Biphenyl Compounds); 0 (Methacrylates); 0 (Onium Compounds); 0 (Photoinitiators, Dental); 0 (Plasma Gases); 0 (ethyl 4-N,N-dimethylaminobenzoate); 0 (para-Aminobenzoates); 10182-84-0 (diphenyliodonium); 6E1I4IV47V (hydroxyethyl methacrylate); 76-22-2 (Camphor); 9007-34-5 (Collagen); 9G34HU7RV0 (Edetic Acid); RAL3591W33 (camphorquinone)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:141203
[St] Status:MEDLINE



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