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  1 / 11090 MEDLINE  
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[PMID]:27774621
[Au] Autor:Schulz U; Reil A; Kiefel V; Bux J; Moog R
[Ad] Endereço:DRK Blood Service North-East, Cottbus, Germany.
[Ti] Título:Evaluation of a new microbeads assay for granulocyte antibody detection.
[So] Source:Transfusion;57(1):70-81, 2017 01.
[Is] ISSN:1537-2995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: To reduce the risk of transfusion-associated acute lung injury (TRALI), a high number of plasma donors were tested for human leukocyte antigen (HLA) and human neutrophil antigen (HNA) antibodies. For HNA antibody detection, the gold standard is a combination of the granulocyte immunofluorescence test (GIFT) and the granulocyte agglutination test (GAT). However, these tests are not suitable for a high-throughput of samples. STUDY DESIGN AND METHODS: To evaluate the new generation of the LABScreen MULTI assay (One Lambda, Inc.), which has special new beads for all the known HNA specificities, including HNA-3a, 97 sera samples containing well-defined HNA antibodies were used. For background testing, we used 91 samples from plasma donors previously identified by GAT, GIFT, and the monoclonal antibody-specific immobilization of granulocyte antigens (MAIGA) assay. RESULTS: Compared with previous tests, the new LABScreen MULTI assay was highly specific for the HNA-1a, HNA-1b, HNA-2, and HNA-3a antibody specificities required to prevent TRALI. Ninety-eight percent of the HNA-1a, HNA-1b, and HNA-2 antibodies could be detected as true positive; and 90% of the HNA-3a antibodies were recognized correctly as positive. False-positive reactions were identified in 5.5% of samples that previously tested negative. CONCLUSION: The detection of HNA-3a antibody specificities could be integrated into the new LABScreen MULTI assay; however, we detected only 90%. In addition, we detected further HNA antibodies, such as HNA-1c, HNA-1d, and some HNA-3b and HNA-4a antibodies. The new generation of LABScreen MULTI is a great step toward feasible high-throughput testing for HNA antibodies. Nevertheless, GIFT and GAT remain the gold-standard methods for the differentiation of rare and currently unknown HNA specificities.
[Mh] Termos MeSH primário: Autoanticorpos/sangue
Isoantígenos/sangue
Microesferas
[Mh] Termos MeSH secundário: Lesão Pulmonar Aguda/sangue
Lesão Pulmonar Aguda/etiologia
Lesão Pulmonar Aguda/prevenção & controle
Testes de Aglutinação/métodos
Anticorpos Monoclonais/química
Feminino
Técnica Direta de Fluorescência para Anticorpo/métodos
Seres Humanos
Masculino
Reação Transfusional
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Autoantibodies); 0 (Isoantigens)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1111/trf.13878


  2 / 11090 MEDLINE  
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[PMID]:29390413
[Au] Autor:Wang L; Lu S; Feng Z; Li L; Niu B; Shuai J; Cao L; Li G; Liu J
[Ad] Endereço:Institute of Pediatric Research.
[Ti] Título:The early examination of combined serum and imaging data under flexible fiberoptic bronchoscopy as a novel predictor for refractory Mycoplasma pneumoniae pneumonia diagnosis.
[So] Source:Medicine (Baltimore);96(50):e9364, 2017 Dec.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The treatment role of flexible bronchoscopy (FOB) for pediatric refractory Mycoplasma pneumoniae pneumonia (RMPP) has been well documented. Besides, the application indication of FOB is also studied in patients with general MPP (GMPP), especially in those with large pulmonary lesions. This study was designed to examine the diagnostic value of bronchoscopic features for RMPP.The FOB and bronchoalveolar lavage (BAL) were adopted for pediatric patients who showed clinical and radiograph indications. On the basis of the final diagnosis on discharge, patients were divided into general and refractory MPP groups. The clinical, laboratory, and bronchoscopic imaging features were retrospectively investigated between these 2 groups.From June 2012 to May 2014, a total of 62 RMPP and 101 GMPP patients were treated with therapeutic bronchoscopy. The comparison analysis showed that the CRP, HBDH, LDH were significantly different between RMPP and GMPP groups (all P < .001). In the bronchoscopic imaging, the mucus plug was significantly more commonly seen in the RMPP group (P < .001). Receiver operating characteristic (ROC) analysis revealed that the combined serum, clinical, and FOB imaging data possessed greater specificity and sensitivity than serum and clinical data alone.Our data suggest that the combined serum, clinical, and bronchoscopic imaging data might serve as a promising predictor for early RMPP diagnosis for pediatric patients with large pulmonary lesions.
[Mh] Termos MeSH primário: Diagnóstico Precoce
Pneumonia por Mycoplasma/diagnóstico
[Mh] Termos MeSH secundário: Testes de Aglutinação
Biomarcadores/sangue
Lavagem Broncoalveolar
Broncoscopia
Pré-Escolar
Diagnóstico Diferencial
Feminino
Seres Humanos
Lactente
Masculino
Pneumonia por Mycoplasma/microbiologia
Valor Preditivo dos Testes
Reação em Cadeia da Polimerase em Tempo Real
Estudos Retrospectivos
Tomografia Computadorizada por Raios X
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180203
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000009364


  3 / 11090 MEDLINE  
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Alves, Leucio Câmara
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[PMID]:28468666
[Au] Autor:Silva JCR; Ferreira F; Dias RA; Ajzenberg D; Marvulo MFV; Magalhães FJR; Filho CDFL; Oliveira S; Soares HS; Feitosa TF; Aizawa J; Alves LC; Mota RA; Dubey JP; Gennari SM; Pena HFJ
[Ad] Endereço:Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, s/n, Dois Irmãos, Recife, PE, 52171-900, Brazil.
[Ti] Título:Cat-rodent Toxoplasma gondii Type II-variant circulation and limited genetic diversity on the Island of Fernando de Noronha, Brazil.
[So] Source:Parasit Vectors;10(1):220, 2017 May 03.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In Brazil, studies on animals and humans in mainland areas have shown that most strains of Toxoplasma gondii are pathogenic to mice and exhibit great genetic variability. RESULTS: In this study, using a set of 11 PCR-RFLP and 15 microsatellite markers, we isolated and genetically characterised T. gondii strains from one cat and three rats on Fernando de Noronha Island. The cat had antibodies to T. gondii, which were revealed using a modified agglutination test (MAT, cut-off 1:25) and the seroprevalence among the 46 rodents was 15.2%. Viable T. gondii was isolated from one cat (TgCatBrFN1), two brown rats (TgRatnoBrFN1 and TgRatnoBrFN2) and one black rat (TgRatraBrFN1). Unlike the strains from mainland Brazil, these isolates were not pathogenic to outbred mice. The genotypes of these strains were compared with strains previously isolated on the island and in mainland Brazil. The analysis based on microsatellite data showed a limited genetic diversity of T. gondii on Fernando de Noronha Island with the majority of strains clustered into the following three groups: type II, III, and Caribbean 1. CONCLUSIONS: There was little variation among strains within the same group, suggesting that the majority of strains circulating on Fernando de Noronha are derived from only a few strains that were recently introduced to the island, likely from imported cats. Except for the strain belonging to the Caribbean 1 group that originates from northeast Brazil, there was little evidence that strains from the other groups were introduced to Fernando de Noronha via mainland Brazil.
[Mh] Termos MeSH primário: Doenças do Gato/parasitologia
Variação Genética
Doenças dos Roedores/parasitologia
Toxoplasma/genética
Toxoplasma/isolamento & purificação
Toxoplasmose Animal/parasitologia
[Mh] Termos MeSH secundário: Testes de Aglutinação
Animais
Animais Selvagens
Anticorpos Antiprotozoários/sangue
Brasil/epidemiologia
Gatos/parasitologia
Genótipo
Seres Humanos
Ilhas
Camundongos
Repetições de Microssatélites
Reação em Cadeia da Polimerase/veterinária
Polimorfismo de Fragmento de Restrição
Ratos
Roedores/parasitologia
Estudos Soroepidemiológicos
Toxoplasma/imunologia
Toxoplasma/patogenicidade
Toxoplasmose Animal/epidemiologia
Toxoplasmose Animal/imunologia
Toxoplasmose Animal/transmissão
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Protozoan)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-017-2150-4


  4 / 11090 MEDLINE  
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[PMID]:29293563
[Au] Autor:Baranova DE; Levinson KJ; Mantis NJ
[Ad] Endereço:Department of Biomedical Sciences, University at Albany, Albany, NY, United States of America.
[Ti] Título:Vibrio cholerae O1 secretes an extracellular matrix in response to antibody-mediated agglutination.
[So] Source:PLoS One;13(1):e0190026, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Vibrio cholerae O1 is one of two serogroups responsible for epidemic cholera, a severe watery diarrhea that occurs after the bacterium colonizes the human small intestine and secretes a potent ADP-ribosylating toxin. Immunity to cholera is associated with intestinal anti-lipopolysaccharide (LPS) antibodies, which are known to inhibit V. cholerae motility and promote bacterial cell-cell crosslinking and aggregation. Here we report that V. cholerae O1 classical and El Tor biotypes produce an extracellular matrix (ECM) when forcibly immobilized and agglutinated by ZAC-3 IgG, an intestinally-derived monoclonal antibody (MAb) against the core/lipid A region of LPS. ECM secretion, as demonstrated by crystal violet staining and scanning electron microscopy, occurred within 30 minutes of antibody exposure and peaked by 3 hours. Non-motile mutants of V. cholerae did not secrete ECM following ZAC-3 IgG exposure, even though they were susceptible to agglutination. The ECM was enriched in O-specific polysaccharide (OSP) but not Vibrio polysaccharide (VPS). Finally, we demonstrate that ECM production by V. cholerae in response to ZAC-3 IgG was associated with bacterial resistant to a secondary complement-mediated attack. In summary, we propose that V. cholerae O1, upon encountering anti-LPS antibodies in the intestinal lumen, secretes an ECM (or O-antigen capsule) possibly as a strategy to shield itself from additional host immune factors and to exit an otherwise inhospitable host environment.
[Mh] Termos MeSH primário: Matriz Extracelular
Vibrio cholerae O1/metabolismo
[Mh] Termos MeSH secundário: Testes de Aglutinação
Ensaio de Imunoadsorção Enzimática
Seres Humanos
Imunoglobulina G/imunologia
Microscopia Eletrônica de Varredura
Antígenos O/imunologia
Vibrio cholerae O1/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Immunoglobulin G); 0 (O Antigens)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190026


  5 / 11090 MEDLINE  
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[PMID]:28743259
[Au] Autor:Wang L; Feng Z; Zhao M; Yang S; Yan X; Guo W; Shi Z; Li G
[Ad] Endereço:Institute of Pediatric Research, Children's Hospital of Hebei Province, 133 Zhonghua South Street, Shijiazhuang, Hebei Province, 050031, China.
[Ti] Título:A comparison study between GeXP-based multiplex-PCR and serology assay for Mycoplasma pneumoniae detection in children with community acquired pneumonia.
[So] Source:BMC Infect Dis;17(1):518, 2017 07 25.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Diagnosis of community-acquired pneumonia (CAP) caused by Mycoplasma pneumoniae (Mp) in children has been hampered by difficulty in obtaining convalescent serum and time constraints. In this study, the two diagnostic assays that targeted respectively on Mp-antibody and Mp-DNA were retrospectively investigated. METHODS: A total of 3146 children were clinically diagnosed to have CAP and were confirmed by chest X-ray during March 2015 to February 2016 in Children's hospital of Hebei Province (China). Both of the sera and sputum samples were collected in 24 h after their admission. The Mp-antibody was examined by the passive particle agglutination assay and a fourfold or greater increase of antibody titers of paired sera or≧1:160 titer of single serum was set as the serology positive. Mp-DNA in the sputum samples was tested by a multiplex-PCR method named GeXP assay (multiplex PCR combined with automated capillary electrophoresis). In order to eliminate the false positive results caused by the asymptomatic carriage after infected by M. pneumoniae, the inconsistent samples were tested by the real-time isothermal transcription-mediated RNA amplification assay (SAT). RESULTS: The inter-rated agreement test was performed in 3146 CAP patients, with a highest kappa value in the school-age children as 0.783. There were 6.29% (198/3146) cases showed inconsistent results determined by GeXP and serology assay. All of the 19 GeXP(+)/Serology (-) samples and a randomly chosen 27 from 179 GeXP(-)/Serology (+) samples were tested by SAT assay, and a 97.8% diagnosis agreement was observed between SAT and GeXP assay, but not with the serology assay. In addition, patients who were detected only by serology or only by multiplex-PCR were significantly younger than those with both methods positive (3.0 and 1.5 years vs. 5.0 years, p < 0.01). The Viral-Mp coinfection accounted for 37.0% (97/262), which was more common in winter and spring (p < 0.05) and in the infantile group (p < 0.01), compared to the pure Mp positive ones. CONCLUSION: In some children CAP cases, the Mp laboratory diagnosis was inconsistent between serology and multiplex-PCR assay. Verified by the SAT assay, the GeXP showed a more sensitive and reliable performance compared with the serology assay. Furthermore, employing the multiplex-PCR could provide more information on the associated pathogens for clinical assessment of CAP.
[Mh] Termos MeSH primário: Infecções Comunitárias Adquiridas/microbiologia
Reação em Cadeia da Polimerase Multiplex/métodos
Mycoplasma pneumoniae/genética
Pneumonia por Mycoplasma/diagnóstico
[Mh] Termos MeSH secundário: Adolescente
Testes de Aglutinação
Anticorpos Antibacterianos/sangue
Criança
Pré-Escolar
China
Técnicas de Laboratório Clínico
Infecções Comunitárias Adquiridas/diagnóstico
Seres Humanos
Lactente
Recém-Nascido
Mycoplasma pneumoniae/patogenicidade
Pneumonia Viral
Estações do Ano
Escarro/microbiologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180120
[Lr] Data última revisão:
180120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2614-3


  6 / 11090 MEDLINE  
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[PMID]:28450662
[Au] Autor:Thengchaisri N; Sinthusingha C; Arthitwong S; Sattasathuchana P
[Ad] Endereço:Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand.
[Ti] Título:Comparative serological investigation between cat and tiger blood for transfusion.
[So] Source:J Vet Med Sci;79(6):1081-1085, 2017 Jun 29.
[Is] ISSN:1347-7439
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Evidence suggests that non-domesticated felids inherited the same AB-erythrocyte antigens as domestic cats. To study the possible compatibility of tiger blood with that of other endangered felidae, blood samples from captive tigers and domestic cats were subjected to an in vitro study. The objectives of this study were to (1) identify whether the captive tigers had blood type AB and (2) determine the compatibility between the blood of captive tigers and that of domestic cats with a similar blood type. The anti-coagulated blood with ethylenediaminetetraacetic acid of 30 tigers was examined to determine blood type, and a crossmatching test was performed between tiger and cat blood. All 30 tigers had blood type A. Tube agglutination tests using tiger plasma with cat erythrocytes resulted in 100% agglutination (n=30) with type B cat erythrocytes and 76.7% agglutination (n=23) with type A cat erythrocytes. The 80% of major and 60% of minor compatibilities between blood from 10 tigers and 10 domestic cats with blood type A were found to pass compatibility tests. Interestingly, 3/10 of the tigers' red blood cell samples were fully compatible with all cat plasmas, and 1/10 of the tiger plasma samples were fully compatible with the type A red cells of domestic cats. Although the result of present findings revealed type-A blood group in the surveyed tigers, the reaction of tiger plasma with Type-A red cell from cats suggested a possibility of other blood type in tigers.
[Mh] Termos MeSH primário: Transfusão de Sangue/veterinária
Gatos/imunologia
Tigres/imunologia
[Mh] Termos MeSH secundário: Testes de Aglutinação/veterinária
Animais
Tipagem e Reações Cruzadas Sanguíneas/veterinária
Gatos/sangue
Feminino
Masculino
Tigres/sangue
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180102
[Lr] Data última revisão:
180102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1292/jvms.16-0630


  7 / 11090 MEDLINE  
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[PMID]:27775481
[Au] Autor:Chikweto A; Sharma RN; Tiwari KP; Verma SK; Calero-Bernal R; Jiang T; Su C; Kwok OC; Dubey JP
[Ad] Endereço:Pathobiology Department, School of Veterinary Medicine, St. George's University, Grenada, West Indies.
[Ti] Título:Isolation and RFLP Genotyping of Toxoplasma gondii in Free-Range Chickens (Gallus domesticus) in Grenada, West Indies, Revealed Widespread and Dominance of Clonal Type III Parasites.
[So] Source:J Parasitol;103(1):52-55, 2017 02.
[Is] ISSN:1937-2345
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The objectives of the present cross-sectional study were to isolate and genotype Toxoplasma gondii in free-range chickens from Grenada, West Indies. Using the modified agglutination test, antibodies to T. gondii were found in 39 (26.9%) of 145 free-range chickens with titers of 25 in 7 chickens, 50 in 6 chickens, 100 in 2 chickens, and 200 or higher in 24 chickens. The hearts of the 39 seropositive chickens were bioassayed in mice; viable T. gondii was isolated from 20 and further propagated in cell culture. Genotyping of T. gondii DNA extracted from cell-cultured tachyzoites using the 10 PCR-restriction fragment length polymorphism (RFLP) markers SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico revealed 4 genotypes, including ToxoDB PCR-RFLP no. 2 (Type III), no. 7, no. 13, and no. 259 (new). These results indicated that T. gondii population genetics in free-range chickens seems to be moderately diverse with ToxoDB no. 2 (Type III) as the most frequent (15/20 = 75%) compared to other genotypes in Grenada.
[Mh] Termos MeSH primário: Galinhas/parasitologia
Técnicas de Genotipagem/veterinária
Polimorfismo de Fragmento de Restrição
Doenças das Aves Domésticas/parasitologia
Toxoplasma/isolamento & purificação
Toxoplasmose Animal/parasitologia
[Mh] Termos MeSH secundário: Testes de Aglutinação/veterinária
Animais
Anticorpos Antiprotozoários/sangue
Bioensaio/veterinária
Estudos Transversais
Genótipo
Granada/epidemiologia
Coração/parasitologia
Camundongos
Reação em Cadeia da Polimerase
Doenças das Aves Domésticas/epidemiologia
Estudos Soroepidemiológicos
Toxoplasma/classificação
Toxoplasma/genética
Toxoplasma/imunologia
Toxoplasmose Animal/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Protozoan)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:180102
[Lr] Data última revisão:
180102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1645/15-945


  8 / 11090 MEDLINE  
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[PMID]:28791815
[Au] Autor:Fiecek B; Chmielewski T; Sadkowska-Todys M; Czerwinski M; Zalewska G; Roguska U; Tylewska-Wierzbanowska S
[Ad] Endereço:National Institute of Public Health - National Institute of Hygiene, Warszawa, Poland.
[Ti] Título:An outbreak of leptospirosis imported from Germany to Poland.
[So] Source:Adv Clin Exp Med;26(3):415-419, 2017 May-Jun.
[Is] ISSN:1899-5276
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Leptospirosis is a zoonotic disease caused by spirochetes of the Leptospiraceae family. In both humans and animals the main route of infection is indirect contact - through water or other products contaminated with urine containing spirochetes. Infection most commonly occurs through ingestion of water or food contaminated with Leptospira spp. OBJECTIVES: The aim of the study was to characterize cases of leptospirosis imported to Poland from Germany in 2014 and to analyze methods that are helpful for making a diagnosis. MATERIAL AND METHODS: The 10 patients examined were reported as suspected leptospirosis cases on the basis of clinical symptoms and epidemiological investigations. They originated from different regions of Poland and had been working together at a strawberry plantation in the Cloppenburg district of Lower Saxony in Germany. Blood and urine samples were tested by PCR and serum samples by serology. All ELISA positive and negative cases were examined using a reference microscopic agglutination test (MAT). RESULTS: In the tested group, 6 individuals (60%) were seropositive according to the ELISA, and 2 of them were confirmed by the MAT. The PCR results for the blood and urine samples were negative. CONCLUSIONS: Using the ELISA in the diagnosis of leptospirosis allowed the disease to be identified much faster, differentiating classes of antibodies and recognizing levels of them that are too low to be detectable by the MAT.
[Mh] Termos MeSH primário: Leptospirose/epidemiologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Testes de Aglutinação/métodos
Animais
Anticorpos Antibacterianos/sangue
Anticorpos Antibacterianos/imunologia
Surtos de Doenças
Ensaio de Imunoadsorção Enzimática/métodos
Feminino
Alemanha/epidemiologia
Seres Humanos
Leptospirose/sangue
Leptospirose/imunologia
Masculino
Meia-Idade
Polônia/epidemiologia
Reação em Cadeia da Polimerase/métodos
Sensibilidade e Especificidade
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.17219/acem/62022


  9 / 11090 MEDLINE  
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[PMID]:28700061
[Au] Autor:Balassiano IT; Vital-Brazil JM; Ramos TMV; Timm LN; Pereira MM
[Ad] Endereço:Laboratório de Zoonoses Bacterianas, Centro de Referência Nacional para Leptospirose, World Health Organization/Pan American Health Organization Centro Colaborador para Leptospirose - Coleção de Leptospira, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
[Ti] Título:Molecular and serological characterization of Leptospira kirschneri serogroup Pomona isolated from a human case in a Brazilian rural area.
[So] Source:Rev Soc Bras Med Trop;50(3):396-398, 2017 May-Jun.
[Is] ISSN:1678-9849
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION:: Leptospirosis is an important health concern in Brazil. Currently, information on the epidemiology of the disease in the rural areas of the country is lacking. METHODS:: Serological and molecular techniques were used to characterize a clinical isolate of Leptospira. RESULTS:: The strain CLEP 00060, isolated from a 59-year-old man in a rural area of Rio Grande do Sul state, Brazil, was identified as belonging to L. kirschneri serogroup Pomona serovar Mozdok. CONCLUSIONS:: This study contributes to the local epidemiological knowledge of leptospirosis, prevention of the disease by vaccines, and improvements in its diagnosis.
[Mh] Termos MeSH primário: Leptospira/classificação
Leptospirose/microbiologia
[Mh] Termos MeSH secundário: Testes de Aglutinação
Brasil
Eletroforese em Gel de Campo Pulsado
Seres Humanos
Leptospira/genética
Leptospira/imunologia
Leptospirose/diagnóstico
Masculino
Meia-Idade
Tipagem de Sequências Multilocus
Filogenia
População Rural
Sorogrupo
Sorotipagem
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE


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[PMID]:28647986
[Au] Autor:Yu F; Wang RN; Chen X; Zheng SF; Wang YY; Chen Y
[Ad] Endereço:Key Laboratory of Clinical in Vitro Diagnostic Techniques of Zhejiang Province, Department of Clinical Laboratory, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
[Ti] Título:[Studies on the serum types and identification efficiency on Diarrheagenic isolated from diarrhea patients, in Zhejiang province].
[So] Source:Zhonghua Liu Xing Bing Xue Za Zhi;38(6):800-804, 2017 Jun 10.
[Is] ISSN:0254-6450
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To investigate the serotypes of Diarrheagenic (DEC) isolated from diarrheal patients in Zhejiang province and to explore the identification efficiency of serological screening methods. Serological agglutination tests were carried out in 696 strains of DEC (through the identification of virulence genes) which were selected from the Infectious Diarrhea Pathogen Monitoring Network Strain Bank of Zhejiang province, from July 2009 to June 2013. Results of virulence genes, serological identification and classification were compared. Among the 696 isolates of DEC, O antigen type was identified in 288 (41.4 ) isolates which belonging to 35 different 'O' serum types. H antigen was seen in 171 (24.6 ) isolates and determined as having 21 types. The agglutination rates of EAEC, ETEC, EPEC and EHEC isolates were 31.9 (130/408), 70.6 (127/180), 31.5 (29/92) and 14.3 (2/14), respectively and belonged to 30, 18, 15 kinds of 'O' sero-groups, respectively. One EHEC isolate was identified as O157∶H7. Serum groups were diverse for EAEC and EPEC, while relatively concentrated on ETEC. Different types of DEC might belong to the same sero-group or type. Among the 74 strains of DEC available for classification serologically, 41 isolates were in consistent with virulence gene identification and another 33 strains were not. The sero-group/type of DEC strains in Zhejiang were varied. Based on the serological screening method alone, DEC classification might end in getting the wrong answer, thus we would recommend the use of virulence gene for the purpose of identification.
[Mh] Termos MeSH primário: Diarreia/microbiologia
Infecções por Escherichia coli/diagnóstico
Escherichia coli/classificação
Escherichia coli/genética
Reação em Cadeia da Polimerase Multiplex/métodos
Antígenos O/análise
[Mh] Termos MeSH secundário: Testes de Aglutinação
Disenteria/microbiologia
Escherichia coli/isolamento & purificação
Infecções por Escherichia coli/microbiologia
Genes Bacterianos
Seres Humanos
Sensibilidade e Especificidade
Sorotipagem
Virulência
Fatores de Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (O Antigens); 0 (Virulence Factors)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170904
[Lr] Data última revisão:
170904
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170627
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.0254-6450.2017.06.022



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