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[PMID]:29223151
[Au] Autor:Ustinov NB; Zavyalova EG; Smirnova IG; Kopylov AM
[Ad] Endereço:Lomonosov Moscow State University, Faculty of Chemistry, Moscow, 119991, Russia. zlenka2006@gmail.com.
[Ti] Título:The Power and Limitations of Influenza Virus Hemagglutinin Assays.
[So] Source:Biochemistry (Mosc);82(11):1234-1248, 2017 Nov.
[Is] ISSN:1608-3040
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Influenza virus hemagglutinins (HAs) are surface proteins that bind to sialic acid residues at the host cell surface and ensure further virus internalization. Development of methods for the inhibition of these processes drives progress in the design of new antiviral drugs. The state of the isolated HA (i.e. combining tertiary structure and extent of oligomerization) is defined by multiple factors, like the HA source and purification method, posttranslational modifications, pH, etc. The HA state affects HA functional activity and significantly impacts the results of numerous HA assays. In this review, we analyze the power and limitations of currently used HA assays regarding the state of HA.
[Mh] Termos MeSH primário: Glicoproteínas de Hemaglutininação de Vírus da Influenza/química
Glicoproteínas de Hemaglutininação de Vírus da Influenza/fisiologia
[Mh] Termos MeSH secundário: Animais
Epitopos/imunologia
Testes de Hemaglutinação
Glicoproteínas de Hemaglutininação de Vírus da Influenza/imunologia
Seres Humanos
Técnicas Imunológicas
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Epitopes); 0 (Hemagglutinin Glycoproteins, Influenza Virus)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180103
[Lr] Data última revisão:
180103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171211
[St] Status:MEDLINE
[do] DOI:10.1134/S0006297917110025


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[PMID]:27774692
[Au] Autor:Awadallah AK; Osman ME; Ibrahim MA; Bernardes ES; Dias-Baruffi M; Konozy EH
[Ad] Endereço:Department of Zoology, Faculty of Science, University of Khartoum, Khartoum, Sudan.
[Ti] Título:Isolation and partial characterization of 3 nontoxic d-galactose-specific isolectins from seeds of Momordica balsamina.
[So] Source:J Mol Recognit;30(2), 2017 Feb.
[Is] ISSN:1099-1352
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Three isolectins denoted hereforth MBaL-30, MBaL-60, and MBaL-80 were isolated from seeds extract of Momordica balsamina by 30%, 60%, and 80% ammonium sulfate saturations, respectively. The native molecular weights of these lectins, as judged by gel filtration, were 108, 56, and 160 kDa, respectively. On SDS-PAGE, under reduced condition, 27 kDa band was obtained for all isolectins. The lectins hemagglutinating activities were variably inhibited by d-galactose (minimum inhibitory concentrations = 12.5mM, 50mM, and 0.391mM, respectively). MBaL-30 and -60 could agglutinate all human blood types with slight preference for the A and O blood groups, whereas MBaL-80 did not agglutinate B and AB blood types. The 3 isolectins were purified from crude seeds extract, collectively, in a single step on the affinity matrix Lactamyl-Seralose 4B; this purified lectin fraction, which contains all isolectins, is termed MBaL. The N-terminal of MBaL till the 25th amino acid was NLSLSELDFSADTYKSFIKNLRKQL, which shares 88% sequence identity with Momordica charantia lectin type-2 ribosomal inactivating protein from Momordica charantia and 50% with momordin II from Momordica balsamina. MBaL retained 100% activity at up to 50°C for 30 minutes. MBaL-30 and MBaL-60 exhibited maximum activities in the pH range between 4 and 8, while MBaL-80 was showing maximum activity in the pH range between 3 and 5. Treatment of MBaL-30 and MBaL-60 with EDTA completely abolished their hemagglutinating activities. Addition of Zn and Fe ions to the ethylenediaminetetraacetic acid-treated MBaL-30 and MBaL-60 lectins did not only regained the loss of activity but also resulted in 200% to 300% increase in activity, respectively. MBaL-30 and -60 agglutinated gram positive Listeria monocytogenes and Staphylococcus aureus, whereas MBaL-30 could merely agglutinate Escherichia coli. None of these lectins could arrest bacterial growth. Addition of MBaL to cancer cell lines (Gastric cancer cell line (AGS) and Gastric cencer cell line (MKN45), Glioblastoma (ECV-304), and Human urinary bladder cancer cell line (U87-MG)) at varying concentrations did not cause statistically significant changes on cell growth and viability.
[Mh] Termos MeSH primário: Momordica/metabolismo
Lectinas de Plantas/análise
Sementes/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Galactose/metabolismo
Testes de Hemaglutinação
Seres Humanos
Peso Molecular
Lectinas de Plantas/química
Lectinas de Plantas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Lectins); X2RN3Q8DNE (Galactose)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171211
[Lr] Data última revisão:
171211
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1002/jmr.2582


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[PMID]:28884263
[Au] Autor:Prezioso C; Scribano D; Bellizzi A; Anzivino E; Rodio DM; Trancassini M; Palamara AT; Pietropaolo V
[Ad] Endereço:Department of Public Health and Infectious Diseases, "Sapienza" University, P.le Aldo Moro, 5, 00185, Rome, Italy.
[Ti] Título:Efficient propagation of archetype JC polyomavirus in COS-7 cells: evaluation of rearrangements within the NCCR structural organization after transfection.
[So] Source:Arch Virol;162(12):3745-3752, 2017 Dec.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:John Cunningham virus (JCPyV) is an ubiquitous human pathogen that causes disease in immunocompromised patients. The JCPyV genome is composed of an early region and a late region, which are physically separated by the non-coding control region (NCCR). The DNA sequence of the NCCR distinguishes two forms of JCPyV, the designated archetype and the prototype, which resulted from a rearrangement of the archetype sequence. To date, the cell culture systems for propagating JCPyV archetype have been very limited in their availability and robustness. Prior to this study, it was demonstrated that JCPyV archetype DNA replicates in COS-7 simian kidney cells expressing SV40 TAg and COS-7 cells expressing HIV-1 Tat. Based on these observations, the present study was conducted to reproduce an in vitro model in COS-7 cells transfected with the JCPyV archetype strain in order to study JCPyV DNA replication and analyze NCCR rearrangements during the viral life cycle. The efficiency of JCPyV replication was evaluated by quantitative PCR (Q-PCR) and by hemagglutination (HA) assay after transfection. In parallel, sequence analysis of JCPyV NCCR was performed. JCPyV efficiently replicated in kidney-derived COS-7 cells, as demonstrated by a progressive increase in viral load and virion particle production after transfection. The archetypal structure of NCCR was maintained during the viral cycle, but two characteristic point mutations were detected 28 days after transfection. This model is a useful tool for analyzing NCCR rearrangements during in vitro replication in cells that are sites of viral persistence, such as tubular epithelial cells of the kidney.
[Mh] Termos MeSH primário: Adaptação Biológica
Rearranjo Gênico
Vírus JC/crescimento & desenvolvimento
Vírus JC/genética
[Mh] Termos MeSH secundário: Animais
Células COS
Cercopithecus aethiops
Testes de Hemaglutinação
Seres Humanos
Mutação Puntual
Reação em Cadeia da Polimerase em Tempo Real
Análise de Sequência de DNA
Transfecção
Cultura de Vírus
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171119
[Lr] Data última revisão:
171119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3542-7


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[PMID]:28732013
[Au] Autor:Luo M; Yang S; Li X; Liu P; Xue J; Zhou X; Su K; Xu X; Qing Y; Qiu J; Li Y
[Ad] Endereço:School of Public Health and Management, Chongqing Medical University, Chongqing, China.
[Ti] Título:The KP1_4563 gene is regulated by the cAMP receptor protein and controls type 3 fimbrial function in Klebsiella pneumoniae NTUH-K2044.
[So] Source:PLoS One;12(7):e0180666, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Klebsiella pneumoniae (K. pneumoniae) is an opportunistic pathogen that can adhere to host cells or extracellular matrix via type 1 and type 3 fimbriae. KP1_4563 is a gene encoding a hypothetical protein in K. pneumoniae NTUH-K2044. KP1_4563 is located between the type 1 and type 3 fimbrial gene clusters and is likely associated with fimbrial function given its putative conserved domains of unknown function (DUF1471). Cyclic AMP receptor protein (CRP) regulates virulence-related gene expression and is a crucial transcriptional regulator in many bacteria. The predicted DNA recognition motif of CRP is present in the KP1_4563 promoter region. This study aimed to investigate the function of KP1_4563 in fimbriae and its transcriptional regulation mechanism by CRP. We generated Kp-Δ4563 mutant and complementation strains. We utilized phenotype and adhesion assays to evaluate the role of KP1_4563 in fimbriae. We conducted quantitative RT-PCR (qRT-PCR), LacZ fusion, electrophoretic mobility shift, and DNase I footprinting assays to study the transcriptional regulation of KP1_4563 gene by CRP. We found that KP1_4563 negatively regulates the function of type 3 fimbriae. Compared with NTUH-K2044, the absence of KP1_4563 enhanced the ability of Kp-Δ4563 to adhere to A549 cells. CRP negatively regulates KP1_4563 by directly binding to its promoter region. KP1_4563 plays an important role in type 3 fimbrial function. This novel insight will assist in the development of strategies for preventing K. pneumoniae infection.
[Mh] Termos MeSH primário: Proteína Receptora de AMP Cíclico/metabolismo
Proteínas de Fímbrias/metabolismo
Klebsiella pneumoniae/genética
Klebsiella pneumoniae/metabolismo
[Mh] Termos MeSH secundário: Aderência Bacteriana/fisiologia
Pegada de DNA
Desoxirribonuclease I/metabolismo
Ensaio de Desvio de Mobilidade Eletroforética
Escherichia coli
Regulação da Expressão Gênica/fisiologia
Testes de Hemaglutinação
Óperon Lac
Mananas/química
Fenótipo
Reação em Cadeia da Polimerase em Tempo Real
Saccharomyces cerevisiae
Deleção de Sequência
Transcrição Genética/fisiologia
beta-Galactosidase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclic AMP Receptor Protein); 0 (Mannans); 147680-16-8 (Fimbriae Proteins); EC 3.1.21.1 (Deoxyribonuclease I); EC 3.2.1.23 (beta-Galactosidase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170722
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180666


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[PMID]:28506795
[Au] Autor:Wong WK; Foo PC; Olivos-Garcia A; Noordin R; Mohamed Z; Othman N; Few LL; Lim BH
[Ad] Endereço:School of Health Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
[Ti] Título:Parallel ELISAs using crude soluble antigen and excretory-secretory antigen for improved serodiagnosis of amoebic liver abscess.
[So] Source:Acta Trop;172:208-212, 2017 Aug.
[Is] ISSN:1873-6254
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Crude soluble antigen (CSA) produced from Entamoeba histolytica trophozoite is conventionally used for serodiagnosis of invasive amoebiasis. However, high background seropositivities by CSA-assay in endemic areas complicate the interpretation of positive result in clinical settings. Instead, incorporating a second assay which indicates active or recent infection into the routine amoebic serology could possibly complement the limitations of CSA-assay. Hence, the present study aimed to evaluate the diagnostic efficacies of indirect ELISAs using CSA and excretory-secretory antigen (ESA) for serodiagnosis of amoebic liver abscess (ALA). Reference standard for diagnosis of ALA at Hospital Universiti Sains Malaysia is based on clinical presentation, radiological imaging and positive indirect haemagglutination assay (titer ≥256). Five groups of human serum samples collected from the hospital included Group I - ALA diagnosed by the reference standard and pus aspirate analysis using real-time PCR (n=10), Group II - ALA diagnosed by the reference standard only (n=41), Group III - healthy control (n=45), Group IV - other diseases control (n=51) and Group V - other infectious diseases control (n=31). For serodiagnosis of ALA serum samples (Group I and II), CSA-ELISA showed sensitivities of 100% for both groups, while ESA-ELISA showed sensitivities of 100% and 88%, respectively. For serodiagnosis of non-ALA serum samples (Group III, IV and V), CSA-ELISA showed specificities of 91%, 75% and 100%, respectively; while ESA-ELISA showed specificities of 96%, 98% and 100%, respectively. Indirect ELISAs using CSA and ESA have shown distinct strength for serodiagnosis of ALA, in terms of sensitivity and specificity, respectively. In conclusion, parallel analysis by both assays improved the overall efficacies of amoebic serology as compared to either single assay.
[Mh] Termos MeSH primário: Anticorpos Antiprotozoários/sangue
Antígenos de Protozoários/imunologia
Entamoeba histolytica
Ensaio de Imunoadsorção Enzimática/métodos
Abscesso Hepático Amebiano/diagnóstico
[Mh] Termos MeSH secundário: Animais
Entamoeba histolytica/genética
Testes de Hemaglutinação
Seres Humanos
Abscesso Hepático Amebiano/sangue
Abscesso Hepático Amebiano/epidemiologia
Abscesso Hepático Amebiano/parasitologia
Malásia/epidemiologia
Reação em Cadeia da Polimerase em Tempo Real
Sensibilidade e Especificidade
Testes Sorológicos/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Protozoan); 0 (Antigens, Protozoan)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170517
[St] Status:MEDLINE


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[PMID]:28381240
[Au] Autor:Balahbib A; Amarir F; Corstjens PL; de Dood CJ; van Dam GJ; Hajli A; Belhaddad M; El Mansouri B; Sadak A; Rhajaoui M; Adlaoui EB
[Ad] Endereço:National Reference Laboratory for Schistosomiasis and Malacology, National Institute of Hygiene, Agdal, Rabat, Morocco. balahbib.abdo@gmail.com.
[Ti] Título:Selecting accurate post-elimination monitoring tools to prevent reemergence of urogenital schistosomiasis in Morocco: a pilot study.
[So] Source:Infect Dis Poverty;6(1):75, 2017 Apr 06.
[Is] ISSN:2049-9957
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: After alleged stop of transmission of schistosomiasis and further down the line in post elimination settings, sensitive tools are required to monitor infection status to prevent potential re-emergence. In Rahala, where transmission cycle of Schistosoma haematobium is interrupted since 2004 but where 30% of snails are still infected by S. bovis, potential human S. bovis infection can't be excluded. As methods based on egg-counts do not provide the required sensitivity, antibody or antigen assays are envisaged as the most appropriate tools for this type of monitoring. METHODS: In this pilot study, the performances of three assays were compared: two commercially available antibody tests (ELISA and haemagglutination format) indicating exposure, and an antigen test (lateral flow strip format) demonstrating active infection. All 37 recruited study participants resided in Rahala (Akka, province Tata, Morocco). Participants had been diagnosed and cured from schistosomiasis in the period between 1983 and 2003. In 2015 these asymptomatic participants provided fresh clinical samples (blood and urine) for analysis with the aforementioned diagnostics tests. RESULTS: No eggs were identified in the urine of the 37 participants. The haemagglutination test indicated 6 antibody positives whereas the ELISA indicated 28 antibody positives, one indecisive and one false positive. ELISA and haemagglutination results matched for 18 individuals, amongst which 5 out of 6 haemagglutination positives. With the antigen test (performed on paired serum and urine samples), serum from two participants (cured 21 and 32 years ago) indicated the presence of low levels of the highly specific Schistosoma circulating anodic antigen (CAA), demonstrating low worm level infections (less than 5 pg/ml corresponding to probably single worm pair). One tested also CAA positive with urine. ELISA indicated the presence of human anti-Schistosoma antibodies in these two CAA positive cases, haemagglutination results were negative. CONCLUSIONS: To prevent reemergence of schistosomiasis in Morocco current monitoring programs require specific protocols that include testing of antibody positives for active infection by the UCP-LF CAA test, the appropriate diagnostic tool to identify Schistosoma low grade infections in travelers, immigrants and assumed cured cases. The test is genus specific will also identify infections related to S. bovis.
[Mh] Termos MeSH primário: Schistosoma/isolamento & purificação
Esquistossomose Urinária/diagnóstico
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Animais
Anticorpos Anti-Helmínticos/sangue
Anticorpos Anti-Helmínticos/urina
Antígenos de Helmintos/sangue
Antígenos de Helmintos/imunologia
Antígenos de Helmintos/urina
Criança
Erradicação de Doenças
Ensaio de Imunoadsorção Enzimática/métodos
Feminino
Testes de Hemaglutinação/métodos
Seres Humanos
Testes Imunológicos/métodos
Masculino
Meia-Idade
Marrocos
Contagem de Ovos de Parasitas
Projetos Piloto
Schistosoma/imunologia
Esquistossomose Urinária/imunologia
Esquistossomose Urinária/parasitologia
Esquistossomose Urinária/prevenção & controle
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Helminth); 0 (Antigens, Helminth)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170512
[Lr] Data última revisão:
170512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE
[do] DOI:10.1186/s40249-017-0289-z


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[PMID]:28369081
[Au] Autor:Egüez KE; Alonso-Padilla J; Terán C; Chipana Z; García W; Torrico F; Gascon J; Lozano-Beltran DF; Pinazo MJ
[Ad] Endereço:Departmental Reference Laboratory, Chuquisaca Departmental Health Service (SEDES-Chuquisaca), Sucre, Bolivia.
[Ti] Título:Rapid diagnostic tests duo as alternative to conventional serological assays for conclusive Chagas disease diagnosis.
[So] Source:PLoS Negl Trop Dis;11(4):e0005501, 2017 Apr.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chagas disease is caused by the parasite Trypanosoma cruzi. It affects several million people, mainly in Latin America, and severe cardiac and/or digestive complications occur in ~30% of the chronically infected patients. Disease acute stage is mostly asymptomatic and infection goes undiagnosed. In the chronic phase direct parasite detection is hampered due to its concealed presence and diagnosis is achieved by serological methods, like ELISA or indirect hemagglutination assays. Agreement in at least two tests must be obtained due to parasite wide antigenic variability. These techniques require equipped labs and trained personnel and are not available in distant regions. As a result, many infected people often remain undiagnosed until it is too late, as the two available chemotherapies show diminished efficacy in the advanced chronic stage. Easy-to-use rapid diagnostic tests have been developed to be implemented in remote areas as an alternative to conventional tests. They do not need electricity, nor cold chain, they can return results within an hour and some even work with whole blood as sample, like Chagas Stat-Pak (ChemBio Inc.) and Chagas Detect Plus (InBIOS Inc.). Nonetheless, in order to qualify a rapidly diagnosed positive patient for treatment, conventional serological confirmation is obligatory, which might risk its start. In this study two rapid tests based on distinct antigen sets were used in parallel as a way to obtain a fast and conclusive Chagas disease diagnosis using whole blood samples. Chagas Stat-Pak and Chagas Detect Plus were validated by comparison with three conventional tests yielding 100% sensitivity and 99.3% specificity over 342 patients seeking Chagas disease diagnosis in a reference centre in Sucre (Bolivia). Combined used of RDTs in distant regions could substitute laborious conventional serology, allowing immediate treatment and favouring better adhesion to it.
[Mh] Termos MeSH primário: Anticorpos Antiprotozoários/sangue
Doença de Chagas/diagnóstico
Testes Diagnósticos de Rotina
Trypanosoma cruzi/imunologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Bioensaio
Bolívia
Doença de Chagas/parasitologia
Criança
Pré-Escolar
Ensaio de Imunoadsorção Enzimática
Feminino
Testes de Hemaglutinação
Seres Humanos
Lactente
Masculino
Meia-Idade
Estudos Prospectivos
Sensibilidade e Especificidade
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Protozoan)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170516
[Lr] Data última revisão:
170516
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005501


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[PMID]:28288910
[Au] Autor:Lu J; Yu Z; Mu C; Li R; Song W; Wang C
[Ad] Endereço:Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo 315211, China.
[Ti] Título:Characterization and functional analysis of a novel C-type lectin from the swimming crab Portunus trituberculatus.
[So] Source:Fish Shellfish Immunol;64:185-192, 2017 May.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:C-type lectins (CTLs) are a family of calcium-dependent carbohydrate-binding proteins. In the present study, a novel C-type lectin (designated as PtCTL1) was identified and characterized from Portunus trituberculatus. The full-length cDNA of PtCTL1 was of 702 bp, containing a 5' untranslated region (UTR) of 91 bp, a 3' UTR of 110 bp with a poly (A) tail, and an open reading frame (ORF) of 501 bp encoding a polypeptide of 166 amino acids with a putative signaling peptide of 21 amino acids. A C-type lectin carbohydrate-recognition domain (CRD) containing four conserved cysteines was identified in the amino acid sequence of PtCTL1. The cDNA fragment encoding the mature peptide of PtCTL1 was recombined into pET-21a(+) with a C-terminal hexa-histidine tag fused in-frame and expressed in Escherichia coli Origami (DE3). The recombinant PtCTL1 (rPtCTL1) can agglutinate all the tested bacteria, including three Gram-positive bacterial strains and three Gram-negative bacterial strains. In addition, erythrocyte agglutination and LPS-binding activity were observed in a Ca -dependent manner. The erythrocyte agglutination was inhibited by EDTA, indicating that PtCTL1 was Ca -dependent. The mRNA transcripts of PtCTL1 were detected mainly in the tissues of hepatopancreas and hemocytes and its levels were significantly up-regulated in hemocytes following Vibrio alginolyticus challenge. These results indicate that PtCTL1 may function as a pattern recognition receptor (PRR) for protecting P. trituberculatus from bacterial infection. Moreover, such findings also provide evidence for further understanding the innate immunology of invertebrate.
[Mh] Termos MeSH primário: Proteínas de Artrópodes/genética
Braquiúros/genética
Braquiúros/imunologia
Lectinas Tipo C/genética
Vibrio alginolyticus/fisiologia
[Mh] Termos MeSH secundário: Aglutinação
Sequência de Aminoácidos
Animais
Proteínas de Artrópodes/química
Proteínas de Artrópodes/metabolismo
Sequência de Bases
Braquiúros/efeitos dos fármacos
Braquiúros/microbiologia
Clonagem Molecular
DNA Complementar/genética
DNA Complementar/metabolismo
Testes de Hemaglutinação
Lectinas Tipo C/química
Lectinas Tipo C/metabolismo
Lipopolissacarídeos/farmacologia
Filogenia
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Alinhamento de Sequência
Distribuição Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arthropod Proteins); 0 (DNA, Complementary); 0 (Lectins, C-Type); 0 (Lipopolysaccharides); 0 (RNA, Messenger)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170629
[Lr] Data última revisão:
170629
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170315
[St] Status:MEDLINE


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[PMID]:28285512
[Au] Autor:Luo H; Li K; Shahzad M; Zhang H; Lan Y; Xiong X
[Ad] Endereço:College of Animal Sciences, Wenzhou Vocational College of Science and Technology, Wenzhou 325006, China.
[Ti] Título:Seroprevalence of Infection in Wild Boars, Wild Rabbits, and Wild Chickens in Hubei Province, China.
[So] Source:Korean J Parasitol;55(1):85-88, 2017 Feb.
[Is] ISSN:1738-0006
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:causes serious infection worldwide in humans and animals. In this study, the seroepidemiology of toxoplasmosis was investigated in wild boars ( ) (n=377), wild rabbits (cape hare, ) (n=331), and wild chickens (red junglefwol, ) (n=571) in 4 forested and country sided area of Hubei province of China. For this, blood samples were collected and tested by indirect hemagglutination test (IHA). The seroprevalence was found to be 7.2%, 5.1%, and 12.6% in wild boars, rabbits, and chickens, respectively, with significant differences among these species. The prevalence of infection in male and female wild boars was found to be 7.9% and 6.5% ( <0.01), in male and female rabbits was 5.6% and 4.9% ( <0.01), and in male and female chickens was 17.1% and 7.7% ( <0.01), respectively, with significant differences between 2 genders of chickens ( <0.01). The findings of this study may help in planning of the prevention measures against infection in wild animals in this area.
[Mh] Termos MeSH primário: Galinhas
Coelhos
Sus scrofa
Toxoplasmose Animal/epidemiologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antiprotozoários/sangue
China/epidemiologia
Feminino
Testes de Hemaglutinação
Masculino
Estudos Soroepidemiológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Protozoan)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170414
[Lr] Data última revisão:
170414
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170314
[St] Status:MEDLINE
[do] DOI:10.3347/kjp.2017.55.1.85


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[PMID]:28268185
[Au] Autor:Xu J; Duan ZL; Guan ZX; Wang YY; Lin C; Zhang TT; Zhang HQ; Qian X; Xia CM
[Ad] Endereço:Department of Parasitology, Medical College of Soochow University, Suzhou, 215123, Jiangsu Province, China. Electronic address: jxu2014@suda.edu.cn.
[Ti] Título:Early detection of circulating DNA of Schistosoma japonicum in sentinel mice models.
[So] Source:Exp Parasitol;176:82-88, 2017 May.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Currently in China, the schistosomiasis control program has shifted its focus from transmission control to the elimination of the disease. Effective forecast and surveillance systems of schistiosomiasis are of great importance for issuing timely and early warnings on risk of infection, and therefore implementing preventive measures to avoid infection. There is great demand in more sensitive and specific methods to improve the surveillance system for early detection of S. japonicum infection in sentinel mice. In this study, we reported a sensitive nested-PCR assay targeting a 303-bp fragment from highly repetitive retrotransposon SjCHGCS19 to detect the S. japonicum DNA in sera of experimental mice. Meanwhile, detection efficacy of the nested-PCR was compared with two conventional methods for field monitoring schistosomiasis such as ELISA and IHA. The nested-PCR assay could detect the specific DNA at 3-day post-infection in sera of mice with 5 cercariae infection, while for ELISA and IHA, both show negative results even after 2 weeks post-infection in mice with 20 cercariae infection. Our results demonstrated the DNA-based assay was more sensitive to make early diagnosis of S. japonicum infection in sentinel mice models, which will improve the early-warning ability of schistosomiasis surveillance system.
[Mh] Termos MeSH primário: DNA de Helmintos/sangue
Schistosoma japonicum/genética
Esquistossomose Japônica/diagnóstico
[Mh] Termos MeSH secundário: Animais
Anticorpos Anti-Helmínticos/sangue
Modelos Animais de Doenças
Ensaio de Imunoadsorção Enzimática
Feminino
Testes de Hemaglutinação
Masculino
Camundongos
Camundongos Endogâmicos ICR
Reação em Cadeia da Polimerase
Distribuição Aleatória
Schistosoma japonicum/imunologia
Schistosoma japonicum/isolamento & purificação
Esquistossomose Japônica/sangue
Esquistossomose Japônica/parasitologia
Sensibilidade e Especificidade
Espécies Sentinelas
Caramujos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Helminth); 0 (DNA, Helminth)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170411
[Lr] Data última revisão:
170411
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170308
[St] Status:MEDLINE



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