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[PMID]:29030070
[Au] Autor:Buffin S; Ikhelef N; Prudent J; Dubayle J; Nougarede N; Varenne MP; Moste C; Legastelois I
[Ad] Endereço:Research and Development, Sanofi Pasteur, Marcy L'Etoile, France. Electronic address: sophie.buffin@sanofi.com.
[Ti] Título:A latex agglutination assay to quantify the amount of hemagglutinin protein in adjuvanted low-dose influenza monovalent vaccines.
[So] Source:J Virol Methods;251:46-53, 2018 Jan.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:To formulate inactivated influenza vaccines, the concentration of hemagglutinin (HA) must be accurately determined. The standard test currently used to measure HA in influenza vaccines is the Single Radial Immunodiffusion (SRID) assay. We developed a very rapid, simple and sensitive alternative quantitative HA assay, namely the Latex Agglutination Assay (LAA). The LAA uses the Spherotest technology, which is based on the agglutination of HA-specific immunoglobulin-coated latex beads. The amount of HA in a sample is calculated from the level of bead agglutination by a simple absorbance measurement at 405nm against a standard curve generated using a monovalent vaccine standard. In less than 2hours, tens of samples could be quantified using the LAA as opposed to 2days for the SRID assay. Ten steps are required to complete an SRID assay as compared to 6 steps for the LAA, from sample preparation through spectrophotometric analysis. Furthermore, the limit of detection of the LAA was found to be approximately 15ng HA/mL, similar to an ELISA, with the quantification of less than 1.8µg HA/mL. The quantification limit of the SRID is usually considered to be approximately 5µg HA/mL. The development of the assay and a comparison of the titers obtained by SRID and LAA for several monovalent vaccines corresponding to various strains were performed. For A/H5N1 and A/H1N1 monovalent vaccines, the LAA was found to be linear and accurate as compared to the SRID. The precision of the LAA was close to that of the standard test, and good reproducibility from one laboratory to another was observed. Moreover, the LAA enabled HA quantification in AlOOH-adjuvanted and in emulsion-adjuvanted low-dose vaccines as well as unadjuvanted vaccines. In conclusion, LAA may be useful to rapidly and accurately measure influenza HA protein in monovalent vaccines, especially in those containing less than 5µg/mL of HA in the presence of an adjuvant.
[Mh] Termos MeSH primário: Glicoproteínas de Hemaglutininação de Vírus da Influenza/análise
Vacinas contra Influenza/imunologia
Testes de Fixação do Látex/métodos
Tecnologia Farmacêutica/métodos
[Mh] Termos MeSH secundário: Fatores de Tempo
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hemagglutinin Glycoproteins, Influenza Virus); 0 (Influenza Vaccines)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171015
[St] Status:MEDLINE


  2 / 4313 MEDLINE  
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[PMID]:29187950
[Au] Autor:Ibrahim OMA; Bilal NE; Osman OF; Magzoub MA
[Ad] Endereço:Department of Microbiology, Faculty of Medicine and Health Sciences, University of El-imam El-mahdi P.O Box 209, Kosti City, Sudan.
[Ti] Título:Assessment of methicillin resistant Aureus detection methods: analytical comparative study.
[So] Source:Pan Afr Med J;27:281, 2017.
[Is] ISSN:1937-8688
[Cp] País de publicação:Uganda
[La] Idioma:eng
[Ab] Resumo:Introduction: The heterogeneous expression of methicillin resistance in (MRSA) affects the efficiency of tests available to detect it. The objective of this study was to assess four phenotypic tests used to detect MRSA. Methods: This is an analytical comparative study conducted among sudanese patients during period from May 2012 to July 2014, strains were isolated and identified by conventional methods, and then confirmed by PCR detection of coagulase gene. PCR detection of mecA gene was used as a gold standard to assess oxacillin resistance screen agar base (ORSAB), oxacillin disc, cefoxitin disc (at different temperatures and incubation periods) and MRSA-latex agglutination test. ATCC 25923 was used as control. Sensitivity and specificity were calculated. Results: MRSA- latex agglutination was the most accurate test; it showed 100% of both sensitivity and specificity, followed by cefoxitin disc with sensitivity of 98.48% and specificity of 100%. However, both of oxacillin disc and oxacillin resistance screen agar base showed less accurate results, and were affected by incubation periods. Oxacillin disc after 24 h incubation both at 30°C and 35°C showed sensitivity and specificity values of 87.88% and 96.23%, respectively. However, after 48h incubation the test at 30°C showed sensitivity and specificity values of 89.39%, and 94.34%, respectively. At 35°C (48h) it showed values of 89.39%, 92.45% respectively. Specificity of ORSAB was more than oxacillin disc at 35°C after 24h incubation 98.11% and 96.23%, respectively. Conclusion: MRSA- latex agglutination and cefoxitin disc diffusion tests are recommended for routine detection of MRSA.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Proteínas de Bactérias/genética
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Proteínas de Ligação às Penicilinas/genética
Infecções Estafilocócicas/diagnóstico
[Mh] Termos MeSH secundário: Cefoxitina/farmacologia
Farmacorresistência Bacteriana
Seres Humanos
Testes de Fixação do Látex
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Oxacilina/farmacologia
Reação em Cadeia da Polimerase/métodos
Sensibilidade e Especificidade
Infecções Estafilocócicas/dietoterapia
Infecções Estafilocócicas/microbiologia
Temperatura Ambiente
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Penicillin-Binding Proteins); 0 (mecA protein, Staphylococcus aureus); 6OEV9DX57Y (Cefoxitin); UH95VD7V76 (Oxacillin)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE
[do] DOI:10.11604/pamj.2017.27.281.9016


  3 / 4313 MEDLINE  
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[PMID]:28867354
[Au] Autor:García VS; Gonzalez VDG; Gugliotta L; Burna A; Demonte A; Arias DG; Cabeza MS; Guerrero SA
[Ad] Endereço:INTEC (CONICET-UNL), Santa Fe, Argentina.
[Ti] Título:Development of a simple and economical diagnostic test for canine leishmaniasis.
[So] Source:Exp Parasitol;182:9-15, 2017 Nov.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Visceral leishmaniasis is a public health problem worldwide. The early diagnosis in dogs is crucial, since they are an epidemiologically relevant reservoir of the disease. The aim of a field study is to early identify the disease allowing rapid intervention to reduce its effects. We propose an immunoagglutination test as a visual in situ method for diagnosis of canine visceral leishmaniasis. Latex-protein complexes were sensitized by covalent coupling of a chimeric recombinant antigen of Leishmania spp. onto polystyrene latex with carboxyl functionality. The reaction time and the antigen concentration under which the immunoagglutination assay shows greater discrimination between the responses of a positive control serum and a negative control serum were determined. Then, the latex-protein complexes were evaluated as a visual diagnostic tool with a panel of 170 sera. The test may be read between 2 and 5 min and can be performed even using sera with elevated concentration of lipids, bilirubin or with variable percentage of hemolysis. The sensitivity, the specificity and the diagnostic accuracy were 78%; 100% and >80%, respectively. The visual immunoagglutination test is of potential application as a method for field studies because it shows results in less than 5 min, it is easy to implement and does not require sophisticated equipment.
[Mh] Termos MeSH primário: Anticorpos Antiprotozoários/sangue
Doenças do Cão/diagnóstico
Testes de Fixação do Látex/veterinária
Leishmania infantum/imunologia
Leishmaniose Visceral/veterinária
[Mh] Termos MeSH secundário: Animais
Antígenos de Protozoários/imunologia
Western Blotting/veterinária
Reservatórios de Doenças
Doenças do Cão/parasitologia
Cães
Leishmaniose Visceral/diagnóstico
Leishmaniose Visceral/parasitologia
Proteínas Recombinantes/imunologia
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Protozoan); 0 (Antigens, Protozoan); 0 (Recombinant Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170905
[St] Status:MEDLINE


  4 / 4313 MEDLINE  
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[PMID]:28558705
[Au] Autor:Ruan Q; Zhu Y; Chen S; Zhu L; Zhang S; Zhang W
[Ad] Endereço:Department of infectious disease, Huashan Hospital of Fudan University, Shanghai, China.
[Ti] Título:Disseminated cryptococcosis with recurrent multiple abscesses in an immunocompetent patient: a case report and literature review.
[So] Source:BMC Infect Dis;17(1):369, 2017 May 30.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Cryptococcus neoformans is frequently present as an opportunistic pathogen mainly affecting immunocompromised populations. Disseminated C. neoformans infection in immunocompetent population is rare and usually involves lung and central nerve system. Cryptococcus from biologic samples can easily grow on routine fungal and bacterial culture media. Besides, cryptococcal latex agglutination test has been established as a reliable diagnostic tool with overall sensitivities of 93-100%. CASE PRESENTATION: We report a rare disseminated cryptococcosis case which presented with chronic recurrent multiple abscess in an immunocompetent male involving skin, lung, spine and iliac fossa without evidence of central nerve system involving. The results of serum cryptococcal latex agglutination tests and standard microbial cultures were negative. The patient underwent empirical anti-bacterial and anti-tuberculosis therapy which turned out to be effectless. Finally, bedside inoculation of the pus was carried out and revealed Cryptococcus neoformans, which was confirmed by polymerase chain reaction. After the administration of anti-fungal drugs including liposomal amphotericin B, the patient recovered from fever and paraplegia. CONCLUSIONS: This case reveals an uncommon pattern of disseminated C. neoformans infection in immunocompetent population presented with chronic multiple abscess and without central nerve system involving. Negative routine microbial cultures may not necessarily rule out cryptococcosis, especially in early stage. Besides, cryptococcal latex agglutination test does have a chance of false negative, which might be related with "capsule-deficiency". Moreover, this phenomenon could be related with low-grade virulence and relative long illness duration.
[Mh] Termos MeSH primário: Anfotericina B/uso terapêutico
Antifúngicos/uso terapêutico
Criptococose/diagnóstico
Criptococose/etiologia
[Mh] Termos MeSH secundário: Abscesso/microbiologia
Idoso
Criptococose/tratamento farmacológico
Cryptococcus neoformans/genética
Cryptococcus neoformans/patogenicidade
Seres Humanos
Hospedeiro Imunocomprometido
Testes de Fixação do Látex
Masculino
Coluna Vertebral/diagnóstico por imagem
Coluna Vertebral/microbiologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (liposomal amphotericin B); 7XU7A7DROE (Amphotericin B)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170601
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2459-9


  5 / 4313 MEDLINE  
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[PMID]:28384252
[Au] Autor:Suttisunhakul V; Pumpuang A; Ekchariyawat P; Wuthiekanun V; Elrod MG; Turner P; Currie BJ; Phetsouvanh R; Dance DA; Limmathurotsakul D; Peacock SJ; Chantratita N
[Ad] Endereço:Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
[Ti] Título:Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the identification of Burkholderia pseudomallei from Asia and Australia and differentiation between Burkholderia species.
[So] Source:PLoS One;12(4):e0175294, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is increasingly used for rapid bacterial identification. Studies of Burkholderia pseudomallei identification have involved small isolate numbers drawn from a restricted geographic region. There is a need to expand the reference database and evaluate B. pseudomallei from a wider geographic distribution that more fully captures the extensive genetic diversity of this species. Here, we describe the evaluation of over 650 isolates. Main spectral profiles (MSP) for 26 isolates of B. pseudomallei (N = 5) and other Burkholderia species (N = 21) were added to the Biotyper database. MALDI-TOF MS was then performed on 581 B. pseudomallei, 19 B. mallei, 6 B. thailandensis and 23 isolates representing a range of other bacterial species. B. pseudomallei originated from northeast and east Thailand (N = 524), Laos (N = 12), Cambodia (N = 14), Hong Kong (N = 4) and Australia (N = 27). All 581 B. pseudomallei were correctly identified, with 100% sensitivity and specificity. Accurate identification required a minimum inoculum of 5 x 107 CFU/ml, and identification could be performed on spiked blood cultures after 24 hours of incubation. Comparison between a dendrogram constructed from MALDI-TOF MS main spectrum profiles and a phylogenetic tree based on recA gene sequencing demonstrated that MALDI-TOF MS distinguished between B. pseudomallei and B. mallei, while the recA tree did not. MALDI-TOF MS is an accurate method for the identification of B. pseudomallei, and discriminates between this and other related Burkholderia species.
[Mh] Termos MeSH primário: Burkholderia pseudomallei/classificação
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
[Mh] Termos MeSH secundário: Ásia
Austrália
Burkholderia pseudomallei/genética
Meios de Cultura
Genes Bacterianos
Testes de Fixação do Látex
Tipagem de Sequências Multilocus
Filogenia
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171118
[Lr] Data última revisão:
171118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0175294


  6 / 4313 MEDLINE  
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[PMID]:28178935
[Au] Autor:Donkor ES; Annan JA; Badoe EV; Dayie NT; Labi AK; Slotved HC
[Ad] Endereço:Department of Medical Microbiology, School of Biomedical and Allied Health Sciences University of Ghana, Accra, Ghana. ericsdon@hotmail.com.
[Ti] Título:Pneumococcal carriage among HIV infected children in Accra, Ghana.
[So] Source:BMC Infect Dis;17(1):133, 2017 Feb 08.
[Is] ISSN:1471-2334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Pneumococcal carriage is the precursor for development of pneumococcal disease, and is also responsible for transmission of the organism from person-to-person. In Africa, little is known about the pneumococcus in relation to people with HIV infection. The aim of the study was to investigate the epidemiology of pneumococcal carriage among HIV infected children visiting a tertiary hospital in Ghana, including the carriage prevalence, risk factors and serotype distribution. METHOD: This was a cross sectional study carried out from February to May, 2015 at the HIV Paediatric Clinic of the Korle-Bu Teaching Hospital in Accra, Ghana. One hundred and eighteen HIV infected children were recruited and nasopharyngeal (NP) swabs were collected from them. Epidemiological data on demographic, household and clinical features of the study participants were also collected. The NP specimens were cultured for Streptococcus pneumoniae and the isolates were serotyped by latex agglutination. The data of the study was analysed using STATA 11 (Strata Corp, College Station, TX, USA). RESULTS: Prevalence of pneumococcal carriage among the HIV infected children was 27.1% (95% CI: 19.1 to 35.1) and the only factor significantly associated with pneumococcal carriage was the presence of respiratory symptoms (OR, 2.63; CI, 1.06-6.53; p = 0.034). The most prevalent pneumococcal serotype among the study participants was serotype 19F (24.4%), followed by 16F (22%). Serotype coverage of the 13-valent Pneumococcal Conjugate Vaccine in this study was 41.5%. Multiple carriage of pneumococcal serotypes among the positive carriage cases was 34.3%. CONCLUSION: Pneumococcal carriage occurred in more than a quarter of the study population and was characterized by predominance of non-vaccine serotypes as well as a high prevalence of multiple carriage. Presence of respiratory symptoms appears to be a major determinant of pneumococcal carriage among the study population.
[Mh] Termos MeSH primário: Portador Sadio/epidemiologia
Infecções por HIV/epidemiologia
Nasofaringe/microbiologia
Infecções Pneumocócicas/epidemiologia
Streptococcus pneumoniae/isolamento & purificação
[Mh] Termos MeSH secundário: Adolescente
Criança
Pré-Escolar
Comorbidade
Tosse/epidemiologia
Estudos Transversais
Dispneia/epidemiologia
Características da Família
Feminino
Gana/epidemiologia
Seres Humanos
Lactente
Testes de Fixação do Látex
Masculino
Faringite/epidemiologia
Infecções Pneumocócicas/prevenção & controle
Vacinas Pneumocócicas/uso terapêutico
Prevalência
Fatores de Risco
Sorogrupo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (13-valent pneumococcal vaccine); 0 (Pneumococcal Vaccines)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170707
[Lr] Data última revisão:
170707
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170210
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2224-0


  7 / 4313 MEDLINE  
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[PMID]:28017861
[Au] Autor:Ben-Abid M; Galaï Y; Habboul Z; Ben-Abdelaziz R; Ben-Sghaier I; Aoun K; Bouratbine A
[Ad] Endereço:Department of Clinical Parasitology, Laboratoire de Recherche LR 11-IPT-06, Parasitologie Médicale, Biotechnologies et Biomolécules, Institut Pasteur de Tunis, Université Tunis El-Manar, Tunis, Tunisia.
[Ti] Título:Diagnosis of Mediterranean visceral leishmaniasis by detection of Leishmania-related antigen in urine and oral fluid samples.
[So] Source:Acta Trop;167:71-72, 2017 Mar.
[Is] ISSN:1873-6254
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Implementation of simple diagnostic tests using non-invasive collection of biological specimens is of great importance in the diagnosis of pediatric visceral leishmaniasis caused by Leishmania infantum. Latex agglutination kit (KAtex ) is widely used in the diagnosis mainly in L. donovani endemic areas. However its utilization in L. infantum endemic regions remains limited and its use on noninvasive biological specimen apart urine was not reported. In this study, KAtex kit was used to detect Leishmania-related antigen in urine and oral fluid of 35 L. infantum visceral leishmaniasis cases and 62 controls including non-infectious disease and infectious disease controls (34 and 28 respectively). Sensitivity and specificity of urine based KAtex were 51.4% and 98.3% respectively, whereas, sensitivity and specificity of oral-fluid based KAtex were 80% and 88.3% respectively. Although, sensitivity of oral-fluid KAtex was high, its specificity varied significantly according to the presence or the absence of an infectious disease (71.4% versus 97%, p=0.01).
[Mh] Termos MeSH primário: Antígenos de Protozoários/análise
Testes de Fixação do Látex
Leishmaniose Visceral/diagnóstico
Saliva/química
[Mh] Termos MeSH secundário: Animais
Estudos de Casos e Controles
Seres Humanos
Lactente
Leishmania infantum/imunologia
Leishmaniose Visceral/urina
Sensibilidade e Especificidade
Tunísia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Protozoan)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161227
[St] Status:MEDLINE


  8 / 4313 MEDLINE  
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[PMID]:27876472
[Au] Autor:Abdelbaset AE; Alhasan H; Salman D; Karram MH; Ellah Rushdi MA; Xuenan X; Igarashi M
[Ad] Endereço:National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, 2-13 Inada-cho, Obihiro, Hokkaido 080-8555, Japan; Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt.
[Ti] Título:Evaluation of recombinant antigens in combination and single formula for diagnosis of feline toxoplasmosis.
[So] Source:Exp Parasitol;172:1-4, 2017 Jan.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cats are the only definitive hosts of Toxoplasma gondii and constitute an essential source of infection to all warm blooded animals and humans. Diagnosis of T. gondii infection in cats is fundamental for proper management and control of infection in humans and animals. In the current study, we have evaluated the diagnostic performance of tachyzoite lysate antigen (TLA) and different T. gondii recombinant antigens including surface antigen 2 (SAG2), dense granule proteins 2, 6, 7, 15 (GRA2, GRA6, GRA7, GRA15) and microneme 10 protein (MIC10) in immunoglobulin G enzyme linked-immunosorbent assay (IgG ELISA) using cat serum samples, with reference to latex agglutination test (LAT). Remarkably, TLA showed better performance than other recombinant antigens in IgG ELISAs as compared to LAT, with concordance and Kappa values of 94.27% and 0.93, respectively. Furthermore, to improve the reactivity of the recombinant antigens, we have developed IgG ELISAs using different combinations with these recombinant antigens. Strikingly, a combination of SAG2 and GRAs has relatively similar performance as TLA evidenced by concordance and Kappa values of 94.27% and 0.81, respectively. The developed ELISA with a combination of recombinant antigens can be used as a promising diagnostic tool for routine testing of T. gondii infection and mass screening in cats. The major advantages of this assay are the high sensitivity and specificity, lower cost, safer production and easiness of standardization in various laboratories worldwide.
[Mh] Termos MeSH primário: Anticorpos Antiprotozoários/sangue
Doenças do Gato/diagnóstico
Toxoplasma/imunologia
Toxoplasmose Animal/diagnóstico
[Mh] Termos MeSH secundário: Animais
Antígenos de Protozoários/genética
Antígenos de Protozoários/imunologia
Gatos
Ensaio de Imunoadsorção Enzimática
Imunoglobulina G/sangue
Testes de Fixação do Látex
Proteínas de Protozoários/genética
Proteínas de Protozoários/imunologia
Proteínas Recombinantes de Fusão/imunologia
Sensibilidade e Especificidade
Testes Sorológicos
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Protozoan); 0 (Antigens, Protozoan); 0 (Immunoglobulin G); 0 (Protozoan Proteins); 0 (Recombinant Fusion Proteins)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170206
[Lr] Data última revisão:
170206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161124
[St] Status:MEDLINE


  9 / 4313 MEDLINE  
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[PMID]:27581781
[Ti] Título:Term 11.
[So] Source:Med Mycol J;57(3):J125, 2016.
[Is] ISSN:1882-0476
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Mh] Termos MeSH primário: Imunocromatografia
Testes de Fixação do Látex/métodos
[Mh] Termos MeSH secundário: Antígenos/análise
Biomarcadores/análise
Líquido da Lavagem Broncoalveolar
Criptococose/diagnóstico
Seres Humanos
Imunocromatografia/métodos
Mananas/imunologia
Polissacarídeos/imunologia
Aspergilose Pulmonar/diagnóstico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens); 0 (Biomarkers); 0 (Mannans); 0 (Polysaccharides); 11078-30-1 (galactomannan); 76082-65-0 (glucuronoxylomannan)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171015
[Lr] Data última revisão:
171015
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160902
[St] Status:MEDLINE
[do] DOI:10.3314/mmj.16.004


  10 / 4313 MEDLINE  
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[PMID]:27436490
[Au] Autor:Gayda J; Reckinger S; Thaben N; Nöckler K; Mayer-Scholl A
[Ad] Endereço:Federal Institute for Risk Assessment, Diedersdorfer Weg 1, 12277 Berlin, Germany. Electronic address: jennifer.gayda@bfr.bund.de.
[Ti] Título:Validation studies of the latex agglutination test for the detection of Trichinella larvae in meat products.
[So] Source:Vet Parasitol;231:150-153, 2016 Nov 15.
[Is] ISSN:1873-2550
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Human trichinellosis is a foodborne disease caused by ingestion of meat infected with Trichinella muscle larvae. To control Trichinella spp. infection in the European Union, all slaughtered pigs from holdings that are not officially recognized as applying controlled housing conditions and other animals susceptible to Trichinella infection and intended for human consumption should be examined by one of the approved digestion methods described in Regulation (EU) No. 2015/1375. In the past, Trichinella outbreaks due to the consumption of cured wild boar or pork products have been described in several European countries, making the identification of the larvae from these products relevant for Trichinella control. Therefore, this study aimed to validate the newly approved latex agglutination test (Trichin-L) for routine testing of cured meat products. The test was validated based on the OIE Guidelines using pork products spiked with Trichinella larvae. The sensitivity of the method varied greatly depending on the investigated meat product and was usually lower than for the gold standard, the magnetic stirrer method. The detection rate reached 80% for three larvae and 60% for one larva in cured pork sausages. A detection rate of 100% for three larvae and 50% for one larva was found in bacon. For frozen samples (-20°C) the Trichin-L kit is similarly sensitive as for cured samples. Further, to determine the performance of the test under field conditions, pork products from regions with known high Trichinella prevalences confiscated by customs authorities at two German international airports were analyzed. Problems associated with the Trichin-L test were incomplete digestion due to fatty ingredients, spices and very dry meat products, resulting in data which could not be evaluated. Therefore, the test is currently not suitable for the detection of Trichinella larvae in cured meat products and needs further adaptation steps to increase both usability and sensitivity.
[Mh] Termos MeSH primário: Parasitologia de Alimentos/métodos
Testes de Fixação do Látex/métodos
Produtos da Carne/parasitologia
Trichinella/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Inspeção de Alimentos
Parasitologia de Alimentos/normas
Testes de Fixação do Látex/normas
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170512
[Lr] Data última revisão:
170512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160721
[St] Status:MEDLINE



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