Base de dados : MEDLINE
Pesquisa : E01.370.225.875.074 [Categoria DeCS]
Referências encontradas : 91 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 10 ir para página                        

  1 / 91 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28711497
[Au] Autor:Ilacqua AN; Shettler JA; Wernke KM; Skalla JK; McQuade KJ
[Ad] Endereço:Department of Biological Sciences, Colorado Mesa University, Grand Junction, CO 81501, USA.
[Ti] Título:Theaflavins from black tea affect growth, development, and motility in Dictyostelium discoideum.
[So] Source:Biochem Biophys Res Commun;491(2):449-454, 2017 Sep 16.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Theaflavins, flavonoids found in black tea, exhibit a variety of health-promoting activities, but the mechanisms by which they act are not clear. Here, we assess the effects of black tea extract and isolated theaflavins on Dictyostelium discoideum, a model organism exhibiting an unusual life cycle relying on conserved pathways involved in human disease. Dictyostelium has been used to characterize the activities of numerous bioactive small molecules, including catechins, from which theaflavins are produced during the preparation of black tea. We show that theaflavins block growth, development, and motility in Dictyostelium, results that suggest catechins and theaflavins exert similar activities in this organism.
[Mh] Termos MeSH primário: Biflavonoides/farmacologia
Camellia sinensis/química
Catequina/farmacologia
Catecóis/farmacologia
Dictyostelium/efeitos dos fármacos
[Mh] Termos MeSH secundário: Cultura Axênica
Biflavonoides/química
Biflavonoides/isolamento & purificação
Catequina/química
Catequina/isolamento & purificação
Catecóis/química
Catecóis/isolamento & purificação
Movimento Celular/efeitos dos fármacos
Movimento Celular/fisiologia
Dictyostelium/crescimento & desenvolvimento
Extratos Vegetais/química
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biflavonoids); 0 (Catechols); 0 (Plant Extracts); 1IA46M0D13 (theaflavin); 8R1V1STN48 (Catechin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170717
[St] Status:MEDLINE


  2 / 91 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28237997
[Au] Autor:Yun YH; Oh MH; Kim JY; Kim SH
[Ad] Endereço:Department of Microbiology, Dankook University, Cheonan 31116, Republic of Korea.
[Ti] Título:, a Hybrid Histidine Kinase Gene, Is Essential for the Sexual Development and Virulence of .
[So] Source:J Microbiol Biotechnol;27(5):1010-1022, 2017 May 28.
[Is] ISSN:1738-8872
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:Hybrid histidine kinase is part of a two-component system that is required for various stress responses and pathogenesis of pathogenic fungi. The gene in human pathogen encodes a hybrid histidine kinase and is important for pathogenesis. In this study, we identified a homolog, , in the maize pathogen by bioinformatics analysis. To explore the role of in the survival of under environmental stresses and its pathogenesis, mutants were constructed by allelic exchange. The growth of mutants was significantly impaired when they were cultured under hyperosmotic stress. The mutants exhibited increased resistance to antifungal agent fludioxonil. In particular, the mutants were unable to produce cytokinesis or conjugation tubes, and to develop fuzzy filaments, resulting in impaired mating between compatible strains. The expression levels of , and , which are involved in the pheromone pathway, were significantly decreased in the mutants. In inoculation tests to the host plant, the mutants showed significantly reduced ability in the production of anthocyanin pigments and tumor development on maize leaves. Overall, the combined results indicated that plays important roles in the survival under hyperosmotic stress, and contributes to cytokinesis, sexual development, and virulence of by regulating the expression of the genes involved in the pheromone pathway.
[Mh] Termos MeSH primário: Genes Fúngicos Tipo Acasalamento/genética
Histidina Quinase/genética
Desenvolvimento Sexual/genética
Ustilago/crescimento & desenvolvimento
Ustilago/patogenicidade
Virulência/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Antocianinas/metabolismo
Antifúngicos/farmacologia
Cultura Axênica
Biologia Computacional
Citocinese
DNA Fúngico/genética
Dioxóis/farmacologia
Escherichia coli/genética
Proteínas Fúngicas/metabolismo
Regulação Fúngica da Expressão Gênica
Proteínas de Grupo de Alta Mobilidade/metabolismo
Histidina Quinase/classificação
Hiperostose
Mutação
Pressão Osmótica
Fenótipo
Feromônios/metabolismo
Filogenia
Doenças das Plantas/microbiologia
Folhas de Planta/metabolismo
Proteínas de Plantas/metabolismo
Pirróis/farmacologia
RNA Mensageiro/análise
Receptores de Feromonas/metabolismo
Alinhamento de Sequência
Estresse Fisiológico/genética
Fatores de Transcrição/metabolismo
Ustilago/efeitos dos fármacos
Zea mays/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Antifungal Agents); 0 (DNA, Fungal); 0 (Dioxoles); 0 (Fungal Proteins); 0 (High Mobility Group Proteins); 0 (Pheromones); 0 (Plant Proteins); 0 (Pyrroles); 0 (RNA, Messenger); 0 (Receptors, Pheromone); 0 (Transcription Factors); 0 (pheromone response factor 1, Ustilago maydis); EC 2.7.13.1 (Histidine Kinase); ENS9J0YM16 (fludioxonil)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170227
[St] Status:MEDLINE
[do] DOI:10.4014/jmb.1702.02001


  3 / 91 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28046131
[Au] Autor:Maes S; Props R; Fitts JP; De Smet R; Vanhaecke F; Boon N; Hennebel T
[Ad] Endereço:Center for Microbial Ecology and Technology (CMET), Department of Biochemical and Microbial Technology, Ghent University, Ghent, Belgium.
[Ti] Título:Biological Recovery of Platinum Complexes from Diluted Aqueous Streams by Axenic Cultures.
[So] Source:PLoS One;12(1):e0169093, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The widespread use of platinum in high-tech and catalytic applications has led to the production of diverse Pt loaded wastewaters. Effective recovery strategies are needed for the treatment of low concentrated waste streams to prevent pollution and to stimulate recovery of this precious resource. The biological recovery of five common environmental Pt-complexes was studied under acidic conditions; the chloro-complexes PtCl42- and PtCl62-, the amine-complex Pt(NH3)4Cl2 and the pharmaceutical complexes cisplatin and carboplatin. Five bacterial species were screened on their platinum recovery potential; the Gram-negative species Shewanella oneidensis MR-1, Cupriavidus metallidurans CH34, Geobacter metallireducens, and Pseudomonas stutzeri, and the Gram-positive species Bacillus toyonensis. Overall, PtCl42- and PtCl62- were completely recovered by all bacterial species while only S. oneidensis and C. metallidurans were able to recover cisplatin quantitatively (99%), all in the presence of H2 as electron donor at pH 2. Carboplatin was only partly recovered (max. 25% at pH 7), whereas no recovery was observed in the case of the Pt-tetraamine complex. Transmission electron microscopy (TEM) revealed the presence of both intra- and extracellular platinum particles. Flow cytometry based microbial viability assessment demonstrated the decrease in number of intact bacterial cells during platinum reduction and indicated C. metallidurans to be the most resistant species. This study showed the effective and complete biological recovery of three common Pt-complexes, and estimated the fate and transport of the Pt-complexes in wastewater treatment plants and the natural environment.
[Mh] Termos MeSH primário: Cultura Axênica
Poluentes Ambientais/análise
Recuperação e Remediação Ambiental
Platina/análise
[Mh] Termos MeSH secundário: Antineoplásicos/análise
Carboplatina/análise
Cisplatino/análise
Cupriavidus
Monitoramento Ambiental
Citometria de Fluxo
Geobacter
Viabilidade Microbiana
Microscopia Eletrônica de Transmissão
Compostos de Platina/análise
Shewanella
Águas Residuais
Purificação da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Environmental Pollutants); 0 (Platinum Compounds); 0 (Waste Water); 12648-47-4 (platinum chloride); 49DFR088MY (Platinum); BG3F62OND5 (Carboplatin); Q20Q21Q62J (Cisplatin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170104
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0169093


  4 / 91 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27866249
[Au] Autor:Esposito-Polesi NP; de Abreu-Tarazi MF; de Almeida CV; Tsai SM; de Almeida M
[Ad] Endereço:Biological Science Department, "Luiz de Queiroz" Superior College of Agriculture, University of São Paulo (ESALQ/USP), Pádua Dias Avenue, 11, P.O. Box 9, Piracicaba, SP, 13418-900, Brazil. esposito.polesi@gmail.com.
[Ti] Título:Investigation of Endophytic Bacterial Community in Supposedly Axenic Cultures of Pineapple and Orchids with Evidence on Abundant Intracellular Bacteria.
[So] Source:Curr Microbiol;74(1):103-113, 2017 Jan.
[Is] ISSN:1432-0991
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Asepsis, defined as the absence of microbial contamination, is one of the most important requirements of plant micropropagation. In long-term micropropagated cultures, there may occasionally occur scattered microorganism growth in the culture medium. These microorganisms are common plant components and are known as latent endophytes. Thus, the aim of this research was to investigate the presence of endophytic bacteria in asymptomatic pineapple and orchid microplants, which were cultivated in three laboratories for 1 year. Isolation and characterization of bacterial isolates, PCR-DGGE from total genomic DNA of microplants and ultrastructural analysis of leaves were performed. In the culture-dependent technique, it was only possible to obtain bacterial isolates from pineapple microplants. In this case, the bacteria genera identified in the isolation technique were Bacillus, Acinetobacter, and Methylobacterium. The scanning electron microscopy and transmission electron microscopy (SEM and TEM) analyses revealed the presence of endophytic bacteria in intracellular spaces in the leaves of pineapple and orchid microplants, independent of the laboratory or cultivation protocol. Our results strongly indicate that there are endophytic bacterial communities inhabiting the microplants before initiation of the in vitro culture and that some of these endophytes persist in their latent form and can also grow in the culture medium even after long-term micropropagation, thus discarding the concept of "truly axenic plants."
[Mh] Termos MeSH primário: Ananas/microbiologia
Bactérias/isolamento & purificação
Endófitos/isolamento & purificação
Orchidaceae/microbiologia
[Mh] Termos MeSH secundário: Ananas/crescimento & desenvolvimento
Cultura Axênica
Bactérias/classificação
Bactérias/genética
Biodiversidade
DNA Bacteriano/genética
DNA Ribossômico/genética
Endófitos/classificação
Endófitos/genética
Orchidaceae/crescimento & desenvolvimento
Filogenia
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161121
[St] Status:MEDLINE
[do] DOI:10.1007/s00284-016-1163-0


  5 / 91 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27862633
[Au] Autor:Gao Y; Tao W; Yan SZ; Chen SL
[Ad] Endereço:College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu, 210023, China.
[Ti] Título:The Life Cycle of Didymium laxifilum and Physarum album on Oat Agar Culture.
[So] Source:J Eukaryot Microbiol;64(4):457-463, 2017 Jul.
[Is] ISSN:1550-7408
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The plasmodial slime molds is the largest group in the phylum Amoebozoa. Its life cycle includes the plasmodial trophic stage and the spore-bearing fruiting bodies. However, only a few species have their complete life cycle known in details so far. This study is the first reporting the morphogenesis of Didymium laxifilum and Physarum album. Spores, from field-collected sporangia, were incubated into hanging drop cultures for viewing germination and axenic oat agar plates for viewing plasmodial development and sporulation. The spores of D. laxifilum and P. album germinated by method of V-shape split and minute pore, respectively. The amoeboflagellates, released from spores, were observed in water film. The phaneroplasmodia of two species developed into a number of sporangia by subhypothallic type on oat agar culture. The main interspecific difference of morphogenesis was also discussed.
[Mh] Termos MeSH primário: Estágios do Ciclo de Vida/fisiologia
Mixomicetos/crescimento & desenvolvimento
Physarum/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Cultura Axênica
Microscopia Eletrônica de Varredura
Morfogênese
Mixomicetos/classificação
Physarum/classificação
Esporos de Protozoários/crescimento & desenvolvimento
Madeira/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161119
[St] Status:MEDLINE
[do] DOI:10.1111/jeu.12383


  6 / 91 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27639667
[Au] Autor:Krishnan R; Menon RR; Likhitha; Busse HJ; Tanaka N; Krishnamurthi S; Rameshkumar N
[Ad] Endereço:Biotechnology Department, National Institute for Interdisciplinary Science and Technology (CSIR), Thiruvananthapuram, 695 019, Kerala, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, 110 001, India.
[Ti] Título:Novosphingobium pokkalii sp nov, a novel rhizosphere-associated bacterium with plant beneficial properties isolated from saline-tolerant pokkali rice.
[So] Source:Res Microbiol;168(2):113-121, 2017 Feb - Mar.
[Is] ISSN:1769-7123
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Pokkali rice varieties are known for their saline tolerance when specifically grown in coastal saline affected agri-fields of southern Kerala. These fields are prone to seawater intrusion. During characterization of phytobeneficial rhizobacteria from this pokkali rice, L3E4 was isolated. This strain showed some plant growth-promoting functions (production of indole acetic acid (IAA), acetoin, and siderophore), biofilm formation and capacity to use a wide range of plant-derived organic compounds. In planta assay under axenic conditions showed a positive effect of L3E4 on pokkali rice growth; importantly, it was able to attach and colonize pokkali rice roots in the presence of natural seawater, a key adaptation required for survival in pokkali rice fields. Phylogenetic analysis using 16S rRNA, recA, and gyrB gene sequences showed that strain L3E4 belongs to the genus Novosphingobium, with Novosphingobium capsulatum GIFU 11526 and Novosphingobium rhizosphaerae JM.1 being the nearest phylogenetic relatives. In addition, DNA-DNA hybridization analysis and phenotypic traits established that this strain belongs to a novel Novosphingobium species, for which we propose the name Novosphingobium pokkalii sp. nov. The type strain is represented by strain L3E4 (=MTCC 12357 = KCTC 42224 ).
[Mh] Termos MeSH primário: Produtos Agrícolas/microbiologia
Oryza/microbiologia
Rizosfera
Sphingomonadaceae/genética
Sphingomonadaceae/isolamento & purificação
[Mh] Termos MeSH secundário: Acetoína/metabolismo
Cultura Axênica
Técnicas de Tipagem Bacteriana
Biofilmes
DNA Girase/genética
DNA Bacteriano/genética
Ácidos Graxos/metabolismo
Ácidos Indolacéticos/metabolismo
Hibridização de Ácido Nucleico
Fosfolipídeos/metabolismo
Filogenia
RNA Ribossômico 16S
Recombinases Rec A/genética
Plantas Tolerantes a Sal/microbiologia
Água do Mar/microbiologia
Análise de Sequência de DNA
Sideróforos/biossíntese
Sphingomonadaceae/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Indoleacetic Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 0 (Siderophores); 6U1S09C61L (indoleacetic acid); BG4D34CO2H (Acetoin); EC 2.7.7.- (Rec A Recombinases); EC 5.99.1.3 (DNA Gyrase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170322
[Lr] Data última revisão:
170322
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160919
[St] Status:MEDLINE


  7 / 91 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:28853769
[Au] Autor:Temraleeva AD; Dronova SA; Moskalenko SV; Didovich SV
[Ti] Título:Modern Methods for Isolation, Purification, and Cultivation of Soil Cyanobacteria.
[So] Source:Mikrobiologiia;85(4):369-380, 2016 Jul.
[Is] ISSN:0026-3656
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:Up-to-date methods for isolation of cyanobacteria from soil samples, removal of accompanying microflora, obtaining axenic strains, and -conditions and media for subsequnt cultivation are reviewed. Char acterization of soil as a specific habitat for cyanobacteria is provided. Comparative analysis of pH and ele- mental composition of the liquid phase of most soil types with the media for cultivating cyanobacteria is car- ried out. The functional role of the major components required for the cultivation of cyanobacteria is de- scribed. The problems associated with isolation, purification, and cultivation of soil cyanobacteria, as well as the relevant solutions, are discussed.
[Mh] Termos MeSH primário: Meios de Cultura/química
Cianobactérias/isolamento & purificação
Microbiologia do Solo
Solo/química
[Mh] Termos MeSH secundário: Cultura Axênica/métodos
Meios de Cultura/farmacologia
Cianobactérias/efeitos dos fármacos
Cianobactérias/crescimento & desenvolvimento
Ecossistema
Concentração de Íons de Hidrogênio
Solo/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Culture Media); 0 (Soil)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170831
[St] Status:MEDLINE


  8 / 91 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27565778
[Au] Autor:Zied DC; Maciel WP; Marques SC; da Silveira E Santos DM; Rinker DL; Dias ES
[Ad] Endereço:Faculdade de Ciências Agrárias e Tecnológicas (FCAT), Universidade Estadual Paulista (UNESP), Dracena, SP, Brazil. dczied@gmail.com.
[Ti] Título:Selection of strains for shiitake production in axenic substrate.
[So] Source:World J Microbiol Biotechnol;32(10):168, 2016 Oct.
[Is] ISSN:1573-0972
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Shiitake mushroom consumption is increasing in Brazil. In addition to the implementation of new production methods, it is also important to increase productivity, quality and reduce production costs. In this study, six commercial Lentinula edodes strains were characterized for genetic diversity (rep-PCR analysis) and mushroom production (yield, number and weight of individual mushrooms) using different substrates and cultural conditions. All strains showed genetic differences by repetitive element palindromic based-polymerase chain reaction (rep-PCR). The richest substrate resulted in the greatest production under both environmental conditions. Strains LE4 and LE6 produced the majority of their mushrooms earlier than the other strains. The highest number of mushrooms was observed in the LE6 strain while the highest weights of individual mushrooms were observed in the LE4 strain. Controlled environmental conditions resulted in superior production for all strains, except for LE4, which had empirically greater yield in the semi-controlled environmental condition.
[Mh] Termos MeSH primário: Cultura Axênica/métodos
Cogumelos Shiitake/crescimento & desenvolvimento
Cogumelos Shiitake/genética
[Mh] Termos MeSH secundário: Agricultura/métodos
Brasil
DNA Fúngico/análise
Variação Genética
Reação em Cadeia da Polimerase/métodos
Locos de Características Quantitativas
Cogumelos Shiitake/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160828
[St] Status:MEDLINE
[do] DOI:10.1007/s11274-016-2115-3


  9 / 91 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27476485
[Au] Autor:Heck K; Machineski GS; Alvarenga DO; Vaz MGMV; Varani AM; Fiore MF
[Ad] Endereço:University of São Paulo, Center for Nuclear Energy in Agriculture, Piracicaba, São Paulo, Brazil.
[Ti] Título:Evaluating methods for purifying cyanobacterial cultures by qPCR and high-throughput Illumina sequencing.
[So] Source:J Microbiol Methods;129:55-60, 2016 Oct.
[Is] ISSN:1872-8359
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Cyanobacteria are commonly found in association with other microorganisms, which constitutes a great challenge during the isolation of cyanobacterial strains. Although several methods have been published for obtaining axenic cyanobacterial cultures, their efficiency is usually evaluated by observing the growth of non-cyanobacteria in culture media. In order to verify whether uncultured bacteria should be a concern during cyanobacterial isolation, this work aimed to detect by molecular methods sequences from cyanobacteria and other bacteria present before and after a technique for obtaining axenic cultures from plating and exposure of Fischerella sp. CENA161 akinetes to the Extran detergent and sodium hypochlorite. Solutions containing 0.5, 1, and 2% sodium hypochlorite were able to remove contaminant bacterial CFUs from the culture. However, qPCR pointed that the quantity of sequences amplified with universal bacteria primers was higher than the number of cyanobacteria-specific sequences before and after treatments. The presence of uncultured bacteria in post-hypochlorite cultures was confirmed by high-throughput Illumina sequencing. These results suggest that culturing may overlook the presence of uncultured bacteria associated to cyanobacterial strains and is not sufficient for monitoring the success of cyanobacterial isolation by itself. Molecular methods such as qPCR could be employed as an additional measure for evaluating axenity in cyanobacterial strains.
[Mh] Termos MeSH primário: Cultura Axênica/métodos
Cianobactérias/genética
Cianobactérias/isolamento & purificação
Sequenciamento de Nucleotídeos em Larga Escala/métodos
Reação em Cadeia da Polimerase em Tempo Real/métodos
[Mh] Termos MeSH secundário: Meios de Cultura/química
Cianobactérias/crescimento & desenvolvimento
Primers do DNA
DNA Bacteriano/genética
Metagenômica/métodos
Filogenia
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Simbiose
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 0 (DNA Primers); 0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160802
[St] Status:MEDLINE


  10 / 91 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27022778
[Au] Autor:Sakoguchi H; Yoshihara A; Izumori K; Sato M
[Ad] Endereço:a Faculty of Agriculture, Department of Applied Biological Science , Kagawa University , Miki , Japan.
[Ti] Título:Screening of biologically active monosaccharides: growth inhibitory effects of d-allose, d-talose, and l-idose against the nematode Caenorhabditis elegans.
[So] Source:Biosci Biotechnol Biochem;80(6):1058-61, 2016 Jun.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We compared the growth inhibitory effects of all aldohexose stereoisomers against the model animal Caenorhabditis elegans. Among the tested compounds, the rare sugars d-allose (d-All), d-talose (d-Tal), and l-idose (l-Ido) showed considerable growth inhibition under both monoxenic and axenic culture conditions. 6-Deoxy-d-All had no effect on growth, which suggests that C6-phosphorylation by hexokinase is essential for inhibition by d-All.
[Mh] Termos MeSH primário: Anti-Helmínticos/farmacologia
Caenorhabditis elegans/efeitos dos fármacos
Glucose/farmacologia
Hexoses/farmacologia
[Mh] Termos MeSH secundário: Animais
Anti-Helmínticos/química
Cultura Axênica
Caenorhabditis elegans/crescimento & desenvolvimento
Glucose/química
Hexoquinase/metabolismo
Hexoses/química
Fosforilação
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthelmintics); 0 (Hexoses); 2152-76-3 (idose); 6038-51-3 (allose); EC 2.7.1.1 (Hexokinase); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170117
[Lr] Data última revisão:
170117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160330
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2016.1146069



página 1 de 10 ir para página                        
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde