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Pesquisa : E01.370.225.875.150.115 [Categoria DeCS]
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[PMID]:29484747
[Au] Autor:Hirad AH; Ahmad J; Alkhedhairy AA; Bahkali AH; Khan ST
[Ad] Endereço:Botany and Microbiology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
[Ti] Título:Bacterial isolates exhibiting multidrug resistance, hemolytic activity, and high 16S rRNA gene similarity with well-known pathogens found in camel milk samples of Riyadh region.
[So] Source:APMIS;126(3):215-226, 2018 Mar.
[Is] ISSN:1600-0463
[Cp] País de publicação:Denmark
[La] Idioma:eng
[Ab] Resumo:Customary consumption of unpasteurized milk by the population in the central Najed region of Saudi Arabia may pose a health risk. Therefore, 80 camel milk samples were collected aseptically from seven different stations of Riyadh region. The biochemical and microbiological properties of these milk samples were determined. Nutrient agar and brain heart infusion agar were used to determine mesophilic aerobic counts (MACs). The MAC in each mL of milk varied from 60 to 16 × 10  CFU/mL on nutrient agar. Based on the colony morphology, 176 colonies were collected from different samples, and these isolates were de-replicated into 80 unique isolates using rep-PCR analysis. Surprisingly, the 16S rRNA sequence analysis of these strains revealed that more than one-third of the collected milk samples contained strains that share maximum sequence similarities with well-known pathogens, such as Brucella, Bacillus anthracis, Listeria monocytogenes, and MRSA. Furthermore, many strains exhibit 16S rRNA gene similarity with opportunistic pathogens such as Citrobacter freundii and Kytococcus schroeteri. Many strains exhibit ß-hemolytic activity and resistant to six different antibiotics. Our study suggested that consumption of raw camel milk from this region constitutes a great health risk.
[Mh] Termos MeSH primário: Bactérias/isolamento & purificação
Leite/química
Leite/microbiologia
[Mh] Termos MeSH secundário: Animais
Bacillus anthracis/genética
Bacillus anthracis/isolamento & purificação
Bactérias/genética
Carga Bacteriana
Brucella/genética
Brucella/isolamento & purificação
Camelus
Citrobacter freundii/genética
Citrobacter freundii/isolamento & purificação
Microbiologia de Alimentos
Seres Humanos
Listeria monocytogenes/genética
Listeria monocytogenes/isolamento & purificação
Staphylococcus aureus Resistente à Meticilina/genética
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Micrococcaceae/genética
Micrococcaceae/isolamento & purificação
Pasteurização
RNA Ribossômico 16S/genética
Arábia Saudita
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180228
[St] Status:MEDLINE
[do] DOI:10.1111/apm.12802


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[PMID]:29458668
[Au] Autor:Al-Ahmad A; Muzafferiy F; Anderson AC; Wölber JP; Ratka-Krüger P; Fretwurst T; Nelson K; Vach K; Hellwig E
[Ad] Endereço:1​Department of Operative Dentistry and Periodontology, Faculty of Medicine, Medical Center - University of Freiburg, Germany.
[Ti] Título:Shift of microbial composition of peri-implantitis-associated oral biofilm as revealed by 16S rRNA gene cloning.
[So] Source:J Med Microbiol;67(3):332-340, 2018 Mar.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Micro-organisms are important triggers of peri-implant inflammation and analysing their diversity is necessary for peri-implantitis treatment. This study aimed to analyse and compare the microbiota associated with individuals with peri-implantitis, as well as clinically healthy implant sites. METHODOLOGY: Subgingival biofilm samples were taken from 10 individuals with peri-implantitis and from at least 1 clinically healthy implant. DNA was extracted and bacterial 16S rRNA genes were amplified using universal primers. After cloning the PCR-products, amplified inserts of positive clones were digested using restriction endonucleases, and the chosen clones were sequenced. The 16S rDNA-sequences were compared to those from the public sequence databases GenBank, EMBL and DDBJ to determine the corresponding taxa. RESULTS: Differing distributions of taxa belonging to the phyla Firmicutes, Bacteroidetes, Fusobacteria, Actinobacteria, Proteobacteria, Synergistetes, Spirochaetae and TM 7 were detected in both the healthy implant (HI) and the peri-implantitis (PI) groups. A significantly higher relative abundance of phylum Bacteroidetes, as well as of the species Fusobacterium nucleatum, were found in the PI group (P<0.05). The putative periodontal red complex (Porphyromonas gingivalis, Tannerella forsythia) was also detected at significantly higher levels in the PI group (P<0.05), whereas the yellow group, as well as the species Veillonella dispar, tended to be associated with the HI group. CONCLUSION: A shift in the healthy subgingival microbiota was shown in peri-implantitis-associated biofilm. Anaerobic Gram-negative periopathogens, including P. gingivalis and T. forsythia, seem to play an important role in peri-implantitis development and should be considered in treatment and prevention strategies.
[Mh] Termos MeSH primário: Bactérias/isolamento & purificação
Biofilmes
Microbiota/genética
Peri-Implantite/microbiologia
RNA Ribossômico 16S/genética
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Bactérias/classificação
Bactérias/genética
Carga Bacteriana
Fenômenos Fisiológicos Bacterianos
Bacteroides/genética
Bacteroides/isolamento & purificação
Feminino
Fusobacterium nucleatum/genética
Fusobacterium nucleatum/isolamento & purificação
Genes de RNAr
Gengiva/microbiologia
Seres Humanos
Masculino
Meia-Idade
Porphyromonas gingivalis/genética
Porphyromonas gingivalis/isolamento & purificação
Prevotella intermedia/genética
Prevotella intermedia/isolamento & purificação
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000682


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[PMID]:28747346
[Au] Autor:Nagalingam G; Vinuesa CG; Britton WJ; Saunders BM
[Ad] Endereço:Tuberculosis Research Program, Centenary Institute, Newtown, New South Wales 2042, Australia.
[Ti] Título:Modulation of Roquin Function in Myeloid Cells Reduces -Induced Inflammation.
[So] Source:J Immunol;199(5):1796-1804, 2017 09 01.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Damaging inflammation is a hallmark of infection, and understanding how this is regulated is important for the development of new therapies to limit excessive inflammation. The E3 ubiquitin ligase, Roquin, is involved in immune regulation; however, its role in immunity to is unknown. To address this, we infected mice with a point mutation in ( ). Aerosol-infected mice showed enhanced control of infection associated with delayed bacterial dissemination and upregulated TNF production in the lungs after 2 wk. However, this early control of infection was not maintained, and by 8 wk postinfection mice demonstrated an increased bacterial burden and dysregulated inflammation in the lungs. As the inflammation in the lungs of the mice could have been influenced by emerging autoimmune conditions that are characteristic of the mice aging, the function of Roquin was examined in immune cell subsets in the absence of autoimmune complications. bacillus Calmette-Guérin-primed T cells transferred into mice provided equivalent protection in the spleen and liver. Interestingly, the transfer of mycobacteria-specific (P25 CD4 TCR transgenic) wild-type spleen cells into mice actually led to enhanced protection with reduced bacterial load, decreased chemokine expression, and reduced inflammation in the lungs compared with transfers into mice expressing intact Roquin. These studies suggest that modulation of Roquin in myeloid cells may reduce both inflammation and bacterial growth during the chronic phase of infection.
[Mh] Termos MeSH primário: Linfócitos T CD4-Positivos/imunologia
Inflamação/imunologia
Pulmão/imunologia
Mycobacterium tuberculosis/fisiologia
Células Mieloides/imunologia
Tuberculose/imunologia
Ubiquitina-Proteína Ligases/metabolismo
[Mh] Termos MeSH secundário: Animais
Carga Bacteriana
Linfócitos T CD4-Positivos/microbiologia
Linfócitos T CD4-Positivos/transplante
Células Cultivadas
Pulmão/microbiologia
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Células Mieloides/microbiologia
Quimeras de Transplante
Fator de Necrose Tumoral alfa/metabolismo
Ubiquitina-Proteína Ligases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Tumor Necrosis Factor-alpha); EC 2.3.2.27 (Rc3h1 protein, mouse); EC 2.3.2.27 (Ubiquitin-Protein Ligases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1602069


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[PMID]:27773684
[Au] Autor:Elkington P; Tebruegge M; Mansour S
[Ad] Endereço:NIHR Southampton Respiratory Biomedical Research Unit, Clinical and Experimental Sciences Academic Unit, Faculty of Medicine, University of Southampton, Southampton, UK. Electronic address: p.elkington@soton.ac.uk.
[Ti] Título:Tuberculosis: An Infection-Initiated Autoimmune Disease?
[So] Source:Trends Immunol;37(12):815-818, 2016 12.
[Is] ISSN:1471-4981
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Tuberculosis (TB) is caused by Mycobacterium tuberculosis (Mtb) and provided original proof that an infectious agent can cause human disease. However, key steps in TB pathogenesis remain poorly understood. We propose that autoimmunity is a critical and overlooked process driving pathology in TB, and present clinical and experimental observations supporting this hypothesis.
[Mh] Termos MeSH primário: Doenças Autoimunes/imunologia
Mycobacterium tuberculosis/imunologia
Tuberculose/imunologia
[Mh] Termos MeSH secundário: Animais
Autoimunidade
Carga Bacteriana/imunologia
Seres Humanos
Hospedeiro Imunocomprometido
Controle de Infecções
Camundongos
Tuberculose/transmissão
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161107
[St] Status:MEDLINE


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[PMID]:29073460
[Au] Autor:Scariot MC; Venturelli GL; Prudêncio ES; Arisi ACM
[Ad] Endereço:CAL CCA UFSC, Food Science and Technology Department, Federal University of Santa Catarina, Rod. Admar Gonzaga, 1346, 88034-001 Florianópolis, SC, Brazil.
[Ti] Título:Quantification of Lactobacillus paracasei viable cells in probiotic yoghurt by propidium monoazide combined with quantitative PCR.
[So] Source:Int J Food Microbiol;264:1-7, 2018 Jan 02.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Propidium monoazide (PMA) coupled with qPCR has been successfully used for specific quantification of viable bacteria cells in diverse matrices food. The present study aimed to develop PMA-qPCR assay for quantification of Lactobacillus paracasei viable cells in probiotic yoghurt. L. paracasei grown in culture medium was submitted to heat treatment at 60°C for different periods of time and probiotic yoghurt containing L. paracasei were prepared and stored at 4°C for 30days. The viable cells were quantified using qPCR and PMA-qPCR assays targeting tuf gene and also by plate counting. Standard curves were prepared and mean efficiency obtained was 94% and 96% (R >0.98) to L. paracasei in culture medium and probiotic yoghurt stored one day, respectively. The limit of detection (LOD) for both samples was 10 genome copies, corresponding to 32.1pg of DNA. For viable cells quantification, standard curves Cq versus log CFU were plotted using mean CFU by plate counting of L. paracasei grown in culture medium and probiotic yoghurt. Results obtained for L. paracasei heat-treated cells were concordant by PMA-qPCR and plate count, CFU decreased as the heat treatment time increased, while qPCR count remained constant. L. paracasei enumerations obtained by qPCR for probiotic yoghurt stored one day and 30days were higher than enumerations by PMA-qPCR for the same samples. The plate count values were similar to CFU values obtained by PMA-qPCR. These results showed that PMA-qPCR is a powerful approach compared with culture-dependent methods for quantification of L. paracasei viable cells in yoghurt. PMA-qPCR allowed reliable obtained results much faster than plate counting.
[Mh] Termos MeSH primário: Azidas/química
Carga Bacteriana/métodos
Lactobacillus paracasei/crescimento & desenvolvimento
Propídio/análogos & derivados
Reação em Cadeia da Polimerase em Tempo Real/métodos
Iogurte/microbiologia
[Mh] Termos MeSH secundário: DNA Bacteriano/análise
Temperatura Alta
Viabilidade Microbiana
Probióticos/análise
Propídio/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Azides); 0 (DNA, Bacterial); 0 (propidium monoazide); 36015-30-2 (Propidium)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171027
[St] Status:MEDLINE


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[PMID]:29304041
[Au] Autor:Gutiérrez-Del-Río I; Marín L; Fernández J; Álvarez San Millán M; Ferrero FJ; Valledor M; Campo JC; Cobián N; Méndez I; Lombó F
[Ad] Endereço:Research Group BIONUC, Departamento de Biología Funcional, Área de Microbiología, University of Oviedo, Oviedo, Principality of Asturias, Spain.
[Ti] Título:Development of a biosensor protein bullet as a fluorescent method for fast detection of Escherichia coli in drinking water.
[So] Source:PLoS One;13(1):e0184277, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Drinking water can be exposed to different biological contaminants from the source, through the pipelines, until reaching the final consumer or industry. Some of these are pathogenic bacteria and viruses which may cause important gastrointestinal or systemic diseases. The microbiological quality of drinking water relies mainly in monitoring three indicator bacteria of faecal origin, Escherichia coli, Enterococcus faecalis and Clostridium perfringens, which serve as early sentinels of potential health hazards for the population. Here we describe the analysis of three chimeric fluorescent protein bullets as biosensor candidates for fast detection of E. coli in drinking water. Two of the chimeric proteins (based on GFP-hadrurin and GFP-pb5 chimera proteins) failed with respect to specificity and/or sensitivity, but the GFP-colS4 chimera protein was able to carry out specific detection of E. coli in drinking water samples in a procedure encompassing about 8 min for final result and this biosensor protein was able to detect in a linear way between 20 and 103 CFU of this bacterium. Below 20 CFU, the system cannot differentiate presence or absence of the target bacterium. The fluorescence in this biosensor system is provided by the GFP subunit of the chimeric protein, which, in the case of the better performing sensor bullet, GFP-colS4 chimera, is covalently bound to a flexible peptide bridge and to a bacteriocin binding specifically to E. coli cells. Once bound to the target bacteria, the excitation step with 395 nm LED light causes emission of fluorescence from the GFP domain, which is amplified in a photomultiplier tube, and finally this signal is converted into an output voltage which can be associated with a CFU value and these data distributed along mobile phone networks, for example. This method, and the portable fluorimeter which has been developed for it, may contribute to reduce the analysis time for detecting E. coli presence in drinking water.
[Mh] Termos MeSH primário: Técnicas Biossensoriais/métodos
Água Potável/microbiologia
Escherichia coli/isolamento & purificação
Microbiologia da Água
[Mh] Termos MeSH secundário: Carga Bacteriana/métodos
Carga Bacteriana/estatística & dados numéricos
Colicinas/química
Colicinas/genética
Escherichia coli/genética
Fluorometria/instrumentação
Proteínas de Fluorescência Verde/química
Proteínas de Fluorescência Verde/genética
Seres Humanos
Proteínas Recombinantes de Fusão/química
Proteínas Recombinantes de Fusão/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Colicins); 0 (Drinking Water); 0 (Recombinant Fusion Proteins); 147336-22-9 (Green Fluorescent Proteins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180210
[Lr] Data última revisão:
180210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184277


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[PMID]:29364606
[Au] Autor:Rumyantseva KV; Kosolapova NG; Kosolapov DB
[Ti] Título:[Relations between Bacterioplankton, Heterotrophic Nanoflagellates, and Virioplankton in the Littoral Zone of a LarRe Plain Reservoir:. ImDact of Bird Colonies.]
[So] Source:Mikrobiologiia;85(5):588-597, 2016 Sep.
[Is] ISSN:0026-3656
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Interactions of the main components of microbial planktonic food web (bacteria, heterotrophic nanoflagellates, and viruses) were studied in a protected overgrown littoral zone of the Rybinsk Reservoir (Upper Volga).. The effect of bird colonial, settlements (the Laridae family) on these processes was deter- mined. The following systems exhibited significant negative correlations: "heterotrophic nanoflagellates- large rod-shaped bacteria" ("predator-prey"), "viruses-bacteriophages-bacterial products" ("parasite-. host") and "heterotrophic nanoflagellates-viruses-bacteriophages." Relations between biotic factors con- trolling bacterial development were more pronounced outside the zone affected by colonial bird settlements. Near the bird colony the role of viruses in mortality of planktonic bacteria increased. Reproduction of bacte- rial cells accelerated in response to the increase in feeding activity of heterotrophic nanoflagellates. Viruses- bacteriophages and heterotrophic nanoflagellates probably eliminate different targets until medium-sized cells become predominant in the bacterial community. Then heterotrophic nanoflagellates consume bacterial cells infected with viruses.
[Mh] Termos MeSH primário: Bactérias/crescimento & desenvolvimento
Charadriiformes/fisiologia
Dinoflagelados/crescimento & desenvolvimento
Processos Heterotróficos/fisiologia
Plâncton/crescimento & desenvolvimento
Vírus/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Bactérias/virologia
Carga Bacteriana
Contagem de Células
Dinoflagelados/microbiologia
Dinoflagelados/virologia
Ecossistema
Cadeia Alimentar
Plâncton/microbiologia
Plâncton/virologia
Tanques/microbiologia
Tanques/virologia
Federação Russa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE


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[PMID]:29364599
[Au] Autor:Mukhanovi VS; Rylkova OA; Churiloval TY; Sakhon EG; Pimenov NV
[Ti] Título:Structure and Seasonal Trophodynamics of Picophytoplankton in Sevastopol Bay and Adjacent Waters (the Black Sea).
[So] Source:Mikrobiologiia;85(5):512-521, 2016 Sep.
[Is] ISSN:0026-3656
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:Abundance and seasonal trophodynamics. (specific growth rate, daily production, and grazing mortality) of the major picophytoplankton components, Synechococcus cyanobacteria (Syn) and picoeukary- otes (Pico-E), were studied at three stations in Sevastopol Bay and adjacent coastal waters (the Black Sea) in 2014 by flow cytometry and the dilution method. Pico- E abundance was shown to increase along the nutrient and pollution gradient from the coastal waters outside the bay (annual average of 7.3 ± 5.4 x 103 cells mL⁻¹) to the eastern corner of the bay (28.7 ± 11.4 x 103 cells mL⁻¹), while no relation was found between the water pollution status.and Syn abundance (9.9 ± 8.7 x 10³ cells mL⁻¹, at all the stations, n=27). Matter flows through the communities (daily production for Syn and Pico-E 0-16.6 and 0-19.3 µg C L- day⁻¹, respec- tively; grazing mortality for Syn and PicoE 0-3.6 and 0-21.2 µg C L⁻¹ day⁻¹, respectively) were comparable to or even exceeded their biomass stocks (<0.05-6.8 and 0.9-26.5 µg C L- for Syn and PicoE, respectively), indicating high biomass turnover rates. The highest flow-to-stock ratio (up to 6 for Syn) and,a significant imbalance between daily production (P) and grazing mortality (G) were observed in the most polluted and eu- trophicated waters of the bay in spring (Pico-E: P/G <.1) and late summer (Syn: P/G > 1). Black River inflow to the bay was hypothesized to be among the mechanisms maintaining.this pronounced and long-term im- balance in the open system without any negative consequences for the picophytoplankton assemlages.
[Mh] Termos MeSH primário: Eutrofização/fisiologia
Fitoplâncton/crescimento & desenvolvimento
Synechococcus/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Carga Bacteriana
Baías
Biomassa
Mar Negro
Contagem de Células
Ecossistema
Fitoplâncton/classificação
Fitoplâncton/metabolismo
Estações do Ano
Synechococcus/metabolismo
Poluição da Água/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE


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[PMID]:27771185
[Au] Autor:Habets MN; van Selm S; van Opzeeland FJ; Simonetti E; Hermans PWM; de Jonge MI; Diavatopoulos DA
[Ad] Endereço:Laboratory of Pediatric Infectious Diseases, Radboud Center for Infectious Diseases, Radboud University Medical Center, Geert Grooteplein 10 (Route 412), P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
[Ti] Título:Role of antibodies and IL17-mediated immunity in protection against pneumococcal otitis media.
[So] Source:Vaccine;34(48):5968-5974, 2016 11 21.
[Is] ISSN:1873-2518
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Widespread vaccination against Streptococcus pneumoniae (the pneumococcus) has significantly reduced pneumococcal disease caused by vaccine serotypes. Despite vaccination, overall pneumococcal colonization rates in children have not reduced and otitis media (OM) by non-vaccine serotypes remains one of the most common childhood infections. Pneumococcal surface protein A (PspA) has been shown to be a promising protein antigen to induce broad protection against pneumococcal colonization. However, its ability to protect against OM remains unclear. Using our previously established mouse model of influenza-virus induced pneumococcal OM, we here show that intranasal vaccination of mice with PspA together with the mucosal adjuvant CTB results in a decrease in pneumococcal load in the middle ears. This decrease correlated with the induction of PspA-specific IgA, a balanced IgG1:IgG2a antibody response and the induction of a mucosal Th17 response. Our data suggests that the IL-17 response to PspA is more important for protection against OM, whilst the presence of antibodies may be less important, as determined in mice deficient in IL-17 signaling or antibody production. Together, these results suggest that mucosal vaccination with PspA may not only protect against colonization, but also against the development of virus-induced pneumococcal OM.
[Mh] Termos MeSH primário: Anticorpos Antibacterianos/imunologia
Interleucina-17/imunologia
Otite Média/imunologia
Otite Média/prevenção & controle
Infecções Pneumocócicas/imunologia
Vacinas Pneumocócicas/imunologia
[Mh] Termos MeSH secundário: Adjuvantes Imunológicos/administração & dosagem
Administração Intranasal
Animais
Anticorpos Antibacterianos/sangue
Carga Bacteriana
Proteínas de Bactérias/imunologia
Modelos Animais de Doenças
Orelha Média/microbiologia
Imunidade nas Mucosas
Imunoglobulina A/imunologia
Interleucina-17/deficiência
Camundongos Endogâmicos BALB C
Otite Média/virologia
Infecções Pneumocócicas/microbiologia
Infecções Pneumocócicas/prevenção & controle
Vacinas Pneumocócicas/administração & dosagem
Streptococcus pneumoniae/imunologia
Streptococcus pneumoniae/isolamento & purificação
Células Th17/imunologia
Vacinação/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Antibodies, Bacterial); 0 (Bacterial Proteins); 0 (Immunoglobulin A); 0 (Interleukin-17); 0 (Pneumococcal Vaccines); 0 (pneumococcal surface protein A)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:28747473
[Au] Autor:Tate AT; Graham AL
[Ad] Endereço:Department of Biological Sciences, Vanderbilt University, Nashville, TN, USA a.tate@vanderbilt.edu.
[Ti] Título:Dissecting the contributions of time and microbe density to variation in immune gene expression.
[So] Source:Proc Biol Sci;284(1859), 2017 Jul 26.
[Is] ISSN:1471-2954
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Widespread differential expression of immunological genes is a hallmark of the response to infection in almost all surveyed taxa. However, several challenges remain in the attempt to connect differences in gene expression with functional outcomes like parasite killing and host survival. For example, temporal gene expression patterns are not always monotonic (unidirectional slope), yielding results that qualitatively depend on the time point selected for analysis. They may also be correlated to microbe density, confounding the strength of an immune response and resistance to parasites. In this study, we analyse these relationships in an mRNA-seq time series of infected with Our results suggest that many extracellular immunological components with known roles in immunity, like antimicrobial peptides and recognition proteins, are highly correlated to microbe load. On the other hand, intracellular components of immunological signalling pathways overwhelmingly show non-monotonic temporal patterns of gene expression, despite the underlying assumption of monotonicity in most ecological and comparative transcriptomics studies that rely on cross-sectional analyses. Our results raise a host of new questions, including to what extent variation in host resistance, infection tolerance and immunopathology can be explained by variation in the slope or sensitivity of these newly characterized patterns.
[Mh] Termos MeSH primário: Carga Bacteriana
Regulação da Expressão Gênica/imunologia
Tribolium/imunologia
[Mh] Termos MeSH secundário: Animais
Bacillus thuringiensis/patogenicidade
Estudos Transversais
Transdução de Sinais
Fatores de Tempo
Tribolium/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180202
[Lr] Data última revisão:
180202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE



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