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  1 / 1873 MEDLINE  
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[PMID]:29287077
[Au] Autor:van Dorp SM; de Greeff SC; Harmanus C; Sanders IMJG; Dekkers OM; Knetsch CW; Kampinga GA; Notermans DW; Kuijper EJ
[Ad] Endereço:Department of Medical Microbiology, Leiden University Medical Center, Leiden, the Netherlands.
[Ti] Título:Ribotype 078 Clostridium difficile infection incidence in Dutch hospitals is not associated with provincial pig farming: Results from a national sentinel surveillance, 2009-2015.
[So] Source:PLoS One;12(12):e0189183, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: It has been suggested that the high incidence of ribotype 078 Clostridium difficile infections (CDI) in the Netherlands is related to pig farming. METHODS: We used data of hospitalised CDI patients (>2yrs of age) diagnosed between May 2009 and May 2015 in 26 hospitals participating in a national sentinel surveillance. We compared clinical and geographical characteristics of 078 CDI to other CDI. We investigated the association between 078 CDI incidence and four indicators of pig farming (piglet, pig, piglet farm and pig farm density) by mixed-effects Poisson regression. We used a space-time permutation model to search for community-onset 078 CDI clusters (using SaTScan). RESULTS: A total of 4,691 CDI were identified. Ribotype 078 was isolated in 493 of 3,756 patients (13.1%) including a typing result. These patients had slightly higher community-onset disease and a 35% increase of 30-day mortality compared to non-078 CDI patients. The pooled overall and 078 incidence rates were 2.82 (95% CI, 2.42-3.29) and 0.26 (95% CI, 0.21-0.31) CDI per 10,000 patients-days respectively. Hospital 078 CDI incidence was not associated with provincial pig (IRR, 0.98; 95% CI, 0.89-1.08), piglet (IRR, 0.95; 95% CI, 0.75-1.19), pig farm (IRR, 1.08; 95% CI, 0.84-1.39), or piglet farm density (IRR, 1.00; 95% CI, 0.56-1.79). No clusters of community-onset ribotype 078 CDI were found. CONCLUSIONS: Our results do not indicate that the ribotype 078 CDI incidence in hospitals is related to pig (farm) or piglet (farm) density. However, transmission beyond provincial borders or in non-hospitalised patients cannot be excluded.
[Mh] Termos MeSH primário: Agricultura
Infecções por Clostridium/epidemiologia
Clostridium difficile/patogenicidade
Ribotipagem/métodos
Vigilância de Evento Sentinela
[Mh] Termos MeSH secundário: Idoso
Animais
Infecções por Clostridium/microbiologia
Infecções por Clostridium/transmissão
Análise por Conglomerados
Feminino
Seres Humanos
Incidência
Masculino
Países Baixos/epidemiologia
Distribuição de Poisson
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171230
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189183


  2 / 1873 MEDLINE  
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[PMID]:29020109
[Au] Autor:Sato J; Nakayama M; Tomita A; Sonoda T; Hasumi M; Miyamoto T
[Ad] Endereço:Safety Science Research, R&D, Kao Corporation, Ichikai, Tochigi, Japan.
[Ti] Título:Evaluation of repetitive-PCR and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for rapid strain typing of Bacillus coagulans.
[So] Source:PLoS One;12(10):e0186327, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In order to establish rapid and accurate typing method for Bacillus coagulans strains which is important for controlling in some canned foods and tea-based beverages manufacturing because of the high-heat resistance of the spores and high tolerance of the vegetative cells to catechins and chemicals, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and repetitive-PCR (rep-PCR) were evaluated. For this purpose, 28 strains of B. coagulans obtained from various culture collections were tested. DNA sequence analyses of the genes encoding 16S rRNA and DNA gyrase classified the test strains into two and three groups, respectively, regardless of their phenotypes. Both MALDI-TOF MS and rep-PCR methods classified the test strains in great detail. Strains classified in each group showed similar phenotypes, such as carbohydrate utilization determined using API 50CH. In particular, the respective two pairs of strains which showed the same metabolic characteristic were classified into the same group by both MALDI-TOF MS and rep-PCR methods separating from the other strains. On the other hand, the other strains which have the different profiles of carbohydrate utilization were separated into different groups by these methods. These results suggested that the combination of MALDI-TOF MS and rep-PCR analyses was advantageous for the rapid and detailed typing of bacterial strains in respect to both phenotype and genotype.
[Mh] Termos MeSH primário: Bacillus coagulans/genética
Tipagem Molecular/métodos
Reação em Cadeia da Polimerase/métodos
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
[Mh] Termos MeSH secundário: Bacillus coagulans/metabolismo
Sequência de Bases
Metabolismo dos Carboidratos
Análise por Conglomerados
DNA Girase/metabolismo
DNA Espaçador Ribossômico/genética
Filogenia
Polimorfismo de Nucleotídeo Único/genética
RNA Ribossômico 16S/genética
Ribotipagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Ribosomal Spacer); 0 (RNA, Ribosomal, 16S); EC 5.99.1.3 (DNA Gyrase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171012
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186327


  3 / 1873 MEDLINE  
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[PMID]:28902923
[Au] Autor:Salazar CL; Reyes C; Atehortua S; Sierra P; Correa MM; Paredes-Sabja D; Best E; Fawley WN; Wilcox M; González Á
[Ad] Endereço:Research Group in Anaerobic Bacteria (GIBA), School of Microbiology, Universidad de Antioquia, Medellín, Colombia.
[Ti] Título:Molecular, microbiological and clinical characterization of Clostridium difficile isolates from tertiary care hospitals in Colombia.
[So] Source:PLoS One;12(9):e0184689, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In Colombia, the epidemiology and circulating genotypes of Clostridium difficile have not yet been described. Therefore, we molecularly characterized clinical isolates of C.difficile from patients with suspicion of C.difficile infection (CDI) in three tertiary care hospitals. C.difficile was isolated from stool samples by culture, the presence of A/B toxins were detected by enzyme immunoassay, cytotoxicity was tested by cell culture and the antimicrobial susceptibility determined. After DNA extraction, tcdA, tcdB and binary toxin (CDTa/CDTb) genes were detected by PCR, and PCR-ribotyping performed. From a total of 913 stool samples collected during 2013-2014, 775 were included in the study. The frequency of A/B toxins-positive samples was 9.7% (75/775). A total of 143 isolates of C.difficile were recovered from culture, 110 (76.9%) produced cytotoxic effect in cell culture, 100 (69.9%) were tcdA+/tcdB+, 11 (7.7%) tcdA-/tcdB+, 32 (22.4%) tcdA-/tcdB- and 25 (17.5%) CDTa+/CDTb+. From 37 ribotypes identified, ribotypes 591 (20%), 106 (9%) and 002 (7.9%) were the most prevalent; only one isolate corresponded to ribotype 027, four to ribotype 078 and four were new ribotypes (794,795, 804,805). All isolates were susceptible to vancomycin and metronidazole, while 85% and 7.7% were resistant to clindamycin and moxifloxacin, respectively. By multivariate analysis, significant risk factors associated to CDI were, staying in orthopedic service, exposure to third-generation cephalosporins and staying in an ICU before CDI symptoms; moreover, steroids showed to be a protector factor. These results revealed new C. difficile ribotypes and a high diversity profile circulating in Colombia different from those reported in America and European countries.
[Mh] Termos MeSH primário: Clostridium difficile/genética
[Mh] Termos MeSH secundário: Idoso
Proteínas de Bactérias/genética
Toxinas Bacterianas/genética
Clostridium difficile/isolamento & purificação
Colômbia
Estudos Transversais
Enterocolite Pseudomembranosa/microbiologia
Enterotoxinas/genética
Feminino
Seres Humanos
Masculino
Testes de Sensibilidade Microbiana
Meia-Idade
Ribotipagem
Fatores de Risco
Centros de Atenção Terciária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Bacterial Toxins); 0 (Enterotoxins); 0 (tcdA protein, Clostridium difficile); 0 (toxB protein, Clostridium difficile)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170914
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184689


  4 / 1873 MEDLINE  
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[PMID]:28891459
[Au] Autor:Zainul NH; Ma ZF; Besari A; Siti Asma H; Rahman RA; Collins DA; Hamid N; Riley TV; Lee YY
[Ad] Endereço:Department of Medicine,School of Medical Sciences,Universiti Sains Malaysia,Kubang Kerian,Kelantan,Malaysia.
[Ti] Título:Prevalence of Clostridium difficile infection and colonization in a tertiary hospital and elderly community of North-Eastern Peninsular Malaysia.
[So] Source:Epidemiol Infect;145(14):3012-3019, 2017 10.
[Is] ISSN:1469-4409
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Little is known about Clostridium difficile infection (CDI) in Asia. The aims of our study were to explore (i) the prevalence, risk factors and molecular epidemiology of CDI and colonization in a tertiary academic hospital in North-Eastern Peninsular Malaysia; (ii) the rate of carriage of C. difficile among the elderly in the region; (iii) the awareness level of this infection among the hospital staffs and students. For stool samples collected from hospital inpatients with diarrhea (n = 76) and healthy community members (n = 138), C. difficile antigen and toxins were tested by enzyme immunoassay. Stool samples were subsequently analyzed by culture and molecular detection of toxin genes, and PCR ribotyping of isolates. To examine awareness among hospital staff and students, participants were asked to complete a self-administered questionnaire. For the hospital and community studies, the prevalence of non-toxigenic C. difficile colonization was 16% and 2%, respectively. The prevalence of CDI among hospital inpatients with diarrhea was 13%. Out of 22 C. difficile strains from hospital inpatients, the toxigenic ribotypes 043 and 017 were most common (both 14%). In univariate analysis, C. difficile colonization in hospital inpatients was significantly associated with greater duration of hospitalization and use of penicillin (both P < 0·05). Absence of these factors was a possible reason for low colonization in the community. Only 3% of 154 respondents answered all questions correctly in the awareness survey. C. difficile colonization is prevalent in a Malaysian hospital setting but not in the elderly community with little or no contact with hospitals. Awareness of CDI is alarmingly poor.
[Mh] Termos MeSH primário: Portador Sadio/epidemiologia
Infecções por Clostridium/epidemiologia
Clostridium difficile/isolamento & purificação
Infecção Hospitalar/epidemiologia
Epidemiologia Molecular
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Portador Sadio/microbiologia
Infecções por Clostridium/complicações
Infecções por Clostridium/microbiologia
Infecção Hospitalar/microbiologia
Diarreia/epidemiologia
Diarreia/etiologia
Diarreia/microbiologia
Fezes/microbiologia
Feminino
Conhecimentos, Atitudes e Prática em Saúde
Seres Humanos
Malásia/epidemiologia
Masculino
Meia-Idade
Prevalência
Ribotipagem
Fatores de Risco
Centros de Atenção Terciária
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170912
[St] Status:MEDLINE
[do] DOI:10.1017/S0950268817002011


  5 / 1873 MEDLINE  
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[PMID]:28800598
[Au] Autor:Blanco N; Foxman B; Malani AN; Zhang M; Walk S; Rickard AH; Eisenberg MC
[Ad] Endereço:Department of Epidemiology, School of Public Health, University of Michigan. Ann Arbor, Michigan, United States of America.
[Ti] Título:An in silico evaluation of treatment regimens for recurrent Clostridium difficile infection.
[So] Source:PLoS One;12(8):e0182815, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Clostridium difficile infection (CDI) is a significant nosocomial infection worldwide, that recurs in as many as 35% of infections. Risk of CDI recurrence varies by ribotype, which also vary in sporulation and germination rates. Whether sporulation/germination mediate risk of recurrence and effectiveness of treatment of recurring CDI remains unclear. We aim to assess the role of sporulation/germination patterns on risk of recurrence, and the relative effectiveness of the recommended tapered/pulsing regimens using an in silico model. METHODS: We created a compartmental in-host mathematical model of CDI, composed of vegetative cells, toxins, and spores, to explore whether sporulation and germination have an impact on recurrence rates. We also simulated the effectiveness of three tapered/pulsed vancomycin regimens by ribotype. RESULTS: Simulations underscored the importance of sporulation/germination patterns in determining pathogenicity and transmission. All recommended regimens for recurring CDI tested were effective in reducing risk of an additional recurrence. Most modified regimens were still effective even after reducing the duration or dosage of vancomycin. However, the effectiveness of treatment varied by ribotype. CONCLUSION: Current CDI vancomycin regimen for treating recurrent cases should be studied further to better balance associated risks and benefits.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Infecções por Clostridium/tratamento farmacológico
Clostridium difficile/efeitos dos fármacos
Modelos Estatísticos
Esporos Bacterianos/efeitos dos fármacos
Vancomicina/farmacologia
[Mh] Termos MeSH secundário: Antibacterianos/farmacocinética
Toxinas Bacterianas/antagonistas & inibidores
Toxinas Bacterianas/biossíntese
Infecções por Clostridium/microbiologia
Infecções por Clostridium/patologia
Clostridium difficile/classificação
Clostridium difficile/genética
Clostridium difficile/crescimento & desenvolvimento
Simulação por Computador
Esquema de Medicação
Cálculos da Dosagem de Medicamento
Seres Humanos
Testes de Sensibilidade Microbiana
Recidiva
Ribotipagem
Esporos Bacterianos/crescimento & desenvolvimento
Esporos Bacterianos/patogenicidade
Vancomicina/farmacocinética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Toxins); 6Q205EH1VU (Vancomycin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170812
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182815


  6 / 1873 MEDLINE  
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[PMID]:28625832
[Au] Autor:Staudacher HM; Lomer MCE; Farquharson FM; Louis P; Fava F; Franciosi E; Scholz M; Tuohy KM; Lindsay JO; Irving PM; Whelan K
[Ad] Endereço:Faculty of Life Sciences and Medicine, Diabetes and Nutritional Sciences Division, King's College London, London, United Kingdom.
[Ti] Título:A Diet Low in FODMAPs Reduces Symptoms in Patients With Irritable Bowel Syndrome and A Probiotic Restores Bifidobacterium Species: A Randomized Controlled Trial.
[So] Source:Gastroenterology;153(4):936-947, 2017 Oct.
[Is] ISSN:1528-0012
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND & AIMS: Dietary restriction of fermentable carbohydrates (a low FODMAP diet) has been reported to reduce symptoms in some patients with irritable bowel syndrome (IBS). We performed a randomized, placebo-controlled study to determine its effects on symptoms and the fecal microbiota in patients with IBS. METHODS: We performed a 2×2 factorial trial of 104 patients with IBS (18-65 years old), based on the Rome III criteria, at 2 hospitals in the United Kingdom. Patients were randomly assigned (blinded) to groups given counselling to follow a sham diet or diet low in FODMAPs for 4 weeks, along with a placebo or multistrain probiotic formulation, resulting in 4 groups (27 receiving sham diet/placebo, 26 receiving sham diet/probiotic, 24 receiving low FODMAP diet /placebo, and 27 receiving low FODMAP diet/probiotic). The sham diet restricted a similar number of staple and non-staple foods as the low FODMAP diet; the diets had similar degrees of difficulty to follow. Dietary counselling was given to patients in all groups and data on foods eaten and compliance were collected. The incidence and severity of 15 gastrointestinal symptoms and overall symptoms were measured daily for 7 days before the study period; along with stool frequency and consistency. At baseline, global and individual symptoms were measured, along with generic and disease-specific health-related quality of life, using standard scoring systems. All data were collected again at 4 weeks, and patients answered questions about adequate symptom relief. Fecal samples were collected at baseline and after 4 weeks and analyzed by quantitative PCR and 16S rRNA sequencing. The co-primary endpoints were adequate relief of symptoms and stool Bifidobacterium species abundance at 4 weeks. RESULTS: There was no significant interaction between the interventions in adequate relief of symptoms (P = .52) or Bifidobacterium species (P = .68). In the intention-to-treat analysis, a higher proportion of patients in the low FODMAP diet had adequate symptom relief (57%) than in the sham diet group (38%), although the difference was not statistically significant (P = .051). In the per-protocol analysis, a significantly higher proportion of patients on the low FODMAP diet had adequate symptom relief (61%) than in the sham diet group (39%) (P = .042). Total mean IBS-Severity Scoring System score was significantly lower for patients on the low FODMAP diet (173 ± 95) than the sham diet (224 ± 89) (P = .001), but not different between those given probiotic (207 ± 98) or placebo (192 ± 93) (P = .721) Abundance of Bifidobacterium species was lower in fecal samples from patients on the low FODMAP diet (8.8 rRNA genes/g) than patients on the sham diet (9.2 rRNA genes/g) (P = .008), but higher in patients given probiotic (9.1 rRNA genes/g) than patients given placebo (8.8 rRNA genes/g) (P = .019). There was no effect of the low FODMAP diet on microbiota diversity in fecal samples. CONCLUSIONS: In a placebo-controlled study of patients with IBS, a low FODMAP diet associates with adequate symptom relief and significantly reduced symptom scores compared with placebo. It is not clear whether changes resulted from collective FODMAP restriction or removal of a single component, such as lactose. Co-administration of the multistrain probiotic increased numbers of Bifidobacterium species, compared with placebo, and might be given to restore these bacteria to patients on a low FODMAP diet. Trial registration no: ISRCTN02275221.
[Mh] Termos MeSH primário: Bifidobacterium/crescimento & desenvolvimento
Dieta com Restrição de Carboidratos
Carboidratos da Dieta/efeitos adversos
Microbioma Gastrointestinal
Intestinos/microbiologia
Síndrome do Intestino Irritável/dietoterapia
Probióticos/uso terapêutico
[Mh] Termos MeSH secundário: Adulto
Bifidobacterium/classificação
Bifidobacterium/genética
Terapia Combinada
Carboidratos da Dieta/metabolismo
Fezes/microbiologia
Feminino
Fermentação
Seres Humanos
Síndrome do Intestino Irritável/diagnóstico
Síndrome do Intestino Irritável/microbiologia
Londres
Masculino
Meia-Idade
Qualidade de Vida
Indução de Remissão
Ribotipagem
Índice de Gravidade de Doença
Inquéritos e Questionários
Fatores de Tempo
Resultado do Tratamento
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Dietary Carbohydrates)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE


  7 / 1873 MEDLINE  
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[PMID]:28414791
[Au] Autor:Dagamac NHA; Rojas C; Novozhilov YK; Moreno GH; Schlueter R; Schnittler M
[Ad] Endereço:Institute of Botany and Landscape Ecology, Ernst Moritz Arndt University Greifswald, Soldmannstr. 15, Greifswald, Germany.
[Ti] Título:Speciation in progress? A phylogeographic study among populations of Hemitrichia serpula (Myxomycetes).
[So] Source:PLoS One;12(4):e0174825, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Myxomycetes (plasmodial slime molds, Amoebozoa) are often perceived as widely distributed, confounding to the "everything is everywhere" hypothesis. To test if gene flow within these spore-dispersed protists is restricted by geographical barriers, we chose the widespread but morphologically unmistakable species Hemitrichia serpula for a phylogeographic study. Partial sequences from nuclear ribosomal RNA genes (SSU) revealed 40 ribotypes among 135 specimens, belonging to three major clades. Each clade is dominated by specimens from a certain region and by one of two morphological varieties which can be differentiated by SEM micrographs. Partial sequences of the protein elongation factor 1 alpha (EF1A) showed each clade to possess a unique combination of SSU and EF1A genotypes. This pattern is best explained assuming the existence of several putative biospecies dominating in a particular geographical region. However, occasional mismatches between molecular data and morphological characters, but as well heterogeneous SSU and heterozygous EF1A sequences, point to ongoing speciation. Environmental niche models suggest that the putative biospecies are rather restricted by geographical barriers than by macroecological conditions. Like other protists, myxomycetes seem to follow the moderate endemicity hypothesis and are in active speciation, which is most likely shaped by limited gene flow and reproductive isolation.
[Mh] Termos MeSH primário: Especiação Genética
Mixomicetos/classificação
Mixomicetos/genética
[Mh] Termos MeSH secundário: Fluxo Gênico
Genes de Protozoários
Variação Genética
Modelos Genéticos
Mixomicetos/ultraestrutura
Fator 1 de Elongação de Peptídeos/genética
Filogenia
Filogeografia
Proteínas de Protozoários/genética
RNA de Protozoário/genética
RNA Ribossômico/genética
Ribotipagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Peptide Elongation Factor 1); 0 (Protozoan Proteins); 0 (RNA, Protozoan); 0 (RNA, Ribosomal)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170509
[Lr] Data última revisão:
170509
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170418
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0174825


  8 / 1873 MEDLINE  
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[PMID]:28355266
[Au] Autor:Nicholas A; Kim YK; Lee WK; Selasi GN; Na SH; Kwon HI; Kim YJ; Lee HS; Song KE; Shin JH; Lee JC
[Ad] Endereço:Department of Microbiology, Kyungpook National University School of Medicine, Daegu, Republic of Korea.
[Ti] Título:Molecular epidemiology and antimicrobial susceptibility of Clostridium difficile isolates from two Korean hospitals.
[So] Source:PLoS One;12(3):e0174716, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Clostridium difficile is one of the main etiological agents causing antibiotic-associated diarrhea. This study investigated the genetic diversity of 70 toxigenic C. difficile isolates from two Korean hospitals by employing toxinotyping, ribotyping, multilocus sequence typing (MLST), and pulsed-field gel electrophoresis (PFGE). Toxin gene amplification resulted in 68 A⁺B⁺ and two A-B+ isolates. Most isolates (95.7-100%) were susceptible to daptomycin, metronidazole, and vancomycin. Seventy C. difficile isolates were classified into five toxinotypes, 19 ribotypes, 16 sequence types (STs), and 33 arbitrary pulsotypes. All C. difficile isolates of ribotype 018 (n = 38) were classified into ST17, which was the most prevalent ST in both hospitals. However, C. difficile isolates of ST17 (ribotype 018) exhibited pulsotypes that differed by hospital. ST2 (ribotype 014/020), 8 (ribotypes 002), 17 (ribotype 018), and 35 (ribotypes 015) were detected in both hospitals, whereas other STs were unique to each hospital. Statistical comparison of the different typing methods revealed that ribotyping and PFGE were highly predictive of STs. In conclusion, our epidemiological study indicates that C. difficile infections in both hospitals are associated with the persistence of endemic clones coupled with the emergence of many unique clones. A combination of MLST with PFGE or ribotyping could be useful for monitoring epidemic C. difficile strains and the emergence of new clones in hospitals.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Infecções por Clostridium/microbiologia
Clostridium difficile/efeitos dos fármacos
Clostridium difficile/genética
Infecção Hospitalar/microbiologia
Testes de Sensibilidade Microbiana/métodos
[Mh] Termos MeSH secundário: Grupo com Ancestrais do Continente Asiático
Infecções por Clostridium/epidemiologia
Infecções por Clostridium/etnologia
Clostridium difficile/classificação
Infecção Hospitalar/epidemiologia
Infecção Hospitalar/etnologia
Daptomicina/farmacologia
Diarreia/epidemiologia
Diarreia/etnologia
Diarreia/microbiologia
Eletroforese em Gel de Campo Pulsado/métodos
Fezes/microbiologia
Genes Bacterianos/genética
Hospitais
Seres Humanos
Metronidazol/farmacologia
Epidemiologia Molecular/métodos
Tipagem de Sequências Multilocus
Reprodutibilidade dos Testes
República da Coreia/epidemiologia
Ribotipagem
Vancomicina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 140QMO216E (Metronidazole); 6Q205EH1VU (Vancomycin); NWQ5N31VKK (Daptomycin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170330
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0174716


  9 / 1873 MEDLINE  
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[PMID]:28335725
[Au] Autor:Lynch M; Walsh TA; Marszalowska I; Webb AE; MacAogain M; Rogers TR; Windle H; Kelleher D; O'Connell MJ; Loscher CE
[Ad] Endereço:Immunomodulation Research Group, School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland.
[Ti] Título:Surface layer proteins from virulent Clostridium difficile ribotypes exhibit signatures of positive selection with consequences for innate immune response.
[So] Source:BMC Evol Biol;17(1):90, 2017 Mar 23.
[Is] ISSN:1471-2148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Clostridium difficile is a nosocomial pathogen prevalent in hospitals worldwide and increasingly common in the community. Sequence differences have been shown to be present in the Surface Layer Proteins (SLPs) from different C. difficile ribotypes (RT) however whether these differences influence severity of infection is still not clear. RESULTS: We used a molecular evolutionary approach to analyse SLPs from twenty-six C. difficile RTs representing different slpA sequences. We demonstrate that SLPs from RT 027 and 078 exhibit evidence of positive selection (PS). We compared the effect of these SLPs to those purified from RT 001 and 014, which did not exhibit PS, and demonstrate that the presence of sites under positive selection correlates with ability to activate macrophages. SLPs from RTs 027 and 078 induced a more potent response in macrophages, with increased levels of IL-6, IL-12p40, IL-10, MIP-1α, MIP-2 production relative to RT 001 and 014. Furthermore, RTs 027 and 078 induced higher expression of CD40, CD80 and MHC II on macrophages with decreased ability to phagocytose relative to LPS. CONCLUSIONS: These results tightly link sequence differences in C. difficile SLPs to disease susceptibility and severity, and suggest that positively selected sites in the SLPs may play a role in driving the emergence of hyper-virulent strains.
[Mh] Termos MeSH primário: Proteínas de Bactérias/imunologia
Infecções por Clostridium/imunologia
Glicoproteínas de Membrana/imunologia
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Infecções por Clostridium/microbiologia
Clostridium difficile/classificação
Clostridium difficile/imunologia
Clostridium difficile/patogenicidade
Seres Humanos
Imunidade Inata
Macrófagos/imunologia
Glicoproteínas de Membrana/genética
Fagocitose
Filogenia
Ribotipagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Membrane Glycoproteins); 0 (S-layer proteins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170627
[Lr] Data última revisão:
170627
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE
[do] DOI:10.1186/s12862-017-0937-8


  10 / 1873 MEDLINE  
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[PMID]:28237369
[Au] Autor:Collins DA; Putsathit P; Elliott B; Riley TV
[Ad] Endereço:Microbiology and Immunology, School of Pathology and Laboratory Medicine, the University of Western Australia, Perth, WA, Australia. Electronic address: deirdre.collins@research.uwa.edu.au.
[Ti] Título:Laboratory-based surveillance of Clostridium difficile strains circulating in the Australian healthcare setting in 2012.
[So] Source:Pathology;49(3):309-313, 2017 Apr.
[Is] ISSN:1465-3931
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Clostridium difficile infection (CDI) has risen in prominence in Australia recently. We conducted laboratory-based surveillance of CDI to examine C. difficile circulating in Australia in October/November 2012. We collected 542 isolates from all States and Territories of Australia except the Northern Territory. The most common ribotypes (RTs) were RTs 014/020 (25.5%), 002 (10.5%), 056 (5.9%) and 070 (4.2%). The survey results were compared with results from a similar Australian survey conducted in 2010. Proportions of RTs 014/020 and 002 remained similar, while RTs 056 (5.9%), 015 (4.1%), 017 (3.3%) and 244 (2.4%) increased in prevalence. Basic clinical and demographic data were available for 338 cases. The majority were healthcare facility-associated (HCFA-CDI, 51.5%) while 17.5% were community-associated (CA-CDI). While no RTs were associated with CA-CDI, RTs 056 and 126 were recently found in Australian production animals, indicating a possible community health threat in Australia.
[Mh] Termos MeSH primário: Infecções por Clostridium/diagnóstico
Infecções por Clostridium/epidemiologia
Clostridium difficile/isolamento & purificação
[Mh] Termos MeSH secundário: Adulto
Idoso
Austrália/epidemiologia
Infecção Hospitalar/diagnóstico
Infecção Hospitalar/epidemiologia
Feminino
Seres Humanos
Masculino
Meia-Idade
Northern Territory
Reação em Cadeia da Polimerase/métodos
Prevalência
Ribotipagem/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170421
[Lr] Data última revisão:
170421
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170227
[St] Status:MEDLINE



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