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  1 / 2845 MEDLINE  
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[PMID]:29214970
[Au] Autor:Kassi FK; Bellet V; Drakulovski P; Krasteva D; Roger F; Valérie BA; Aboubakar T; Doumbia A; Kouakou GA; Delaporte E; Reynes J; Yavo W; Menan HIE; Bertout S
[Ad] Endereço:1​Laboratoire de Parasitologie et de Mycologie - CeDReS (Centre de Diagnostic et de Recherche sur le SIDA et les Autres Maladies Infectieuses), UFR Pharmacie, CHU de Treichville, Université Félix Houphouët Boigny, Abidjan, Ivory Coast.
[Ti] Título:Comparative typing analyses of clinical and environmental strains of the Cryptococcus neoformans/Cryptococcus gattii species complex from Ivory Coast.
[So] Source:J Med Microbiol;67(1):87-96, 2018 Jan.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: The aim of this study was to assess the biotope of the Cryptococcus neoformans/Cryptococcus gattii species complex from Ivory Coast, and clarify the possible epidemiological relationship between environmental and clinical strains. METHODOLOGY: Samples from Eucalyptus camaldulensis (n=136), Mangifera indica (n=13) and pigeon droppings (n=518) were collected from different sites close to the living environment of Ivorian HIV patients with cryptococcosis (n=10, 50 clinical strains). Clinical and environmental strains were characterized by molecular serotyping and genotyping [RFLP analysis of the URA5 gene, (GACA)4, (GTG)5 and M13 PCR fingerprinting] and compared.Results/Key findings. Environmental strains were recovered only from the pigeon droppings. In vitro susceptibility profiles showed that all strains were susceptible to fluconazole, flucytosine and amphotericin B. All environmental strains consisted of C. neoformans (A, AFLP1/VNI), whereas clinical strains included C. neoformans (A, AFLP1/VNI), C. neoformans x Cryptococcus deneoformans hybrids (AD, AFLP3/VNIII) and Cryptococcus deuterogattii (B, AFLP6/VGII). Two patients were co-infected with both C. neoformans and C. neoformans x C. deneoformans hybrids. We noticed a low genetic diversity among the environmental samples compared to the high diversity of the clinical samples. Some clinical strains were genetically more similar to environmental strains than to other clinical strains, including those from the same patient. CONCLUSION: These results provide new information on the ecology and epidemiology of the C. neoformans/C. gattii species complex in Ivory Coast.
[Mh] Termos MeSH primário: Cryptococcus gattii/genética
Cryptococcus neoformans/genética
[Mh] Termos MeSH secundário: Adulto
Anfotericina B/uso terapêutico
Antifúngicos/uso terapêutico
Cloranfenicol/uso terapêutico
Costa do Marfim
Criptococose/microbiologia
DNA Fúngico/genética
Microbiologia Ambiental
Feminino
Fluconazol/uso terapêutico
Flucitosina/uso terapêutico
Genótipo
Infecções por HIV/microbiologia
Seres Humanos
Masculino
Testes de Sensibilidade Microbiana/métodos
Meia-Idade
Tipagem Molecular/métodos
Técnicas de Tipagem Micológica/métodos
Estudos Prospectivos
Sorotipagem/métodos
Adulto Jovem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (DNA, Fungal); 66974FR9Q1 (Chloramphenicol); 7XU7A7DROE (Amphotericin B); 8VZV102JFY (Fluconazole); D83282DT06 (Flucytosine)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171227
[Lr] Data última revisão:
171227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000654


  2 / 2845 MEDLINE  
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[PMID]:28895520
[Au] Autor:Kobayashi R; Kanti A; Kawasaki H
[Ad] Endereço:1​Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Chiba, Japan.
[Ti] Título:Three novel species of d-xylose-assimilating yeasts, Barnettozyma xylosiphila sp. nov., Barnettozyma xylosica sp. nov. and Wickerhamomyces xylosivorus f.a., sp. nov.
[So] Source:Int J Syst Evol Microbiol;67(10):3971-3976, 2017 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study describes three novel xylose-assimilating yeasts, which were isolated from decayed wood collected from Bung Hatta Botanical Garden in West Sumatra and Cibodas Botanic Garden in West Java, or from litter from Eka Karya Bali Botanic Garden in Bali, Indonesia. Phylogenetic analysis was performed based on the sequences of the D1/D2 domains of the large ribosomal subunit (LSU), the small ribosomal subunit (SSU), the internal transcribed spacer (ITS) and elongation factor-1α (EF-1α), and the three strains were found to represent three novel species belonging to genera Barnettozyma or Wickerhamomyces. The morphological, biochemical and physiological characteristics indicated that the strains were distinct from other closely related species. Strains 13Y206 and 14Y196 belonging to the Barnettozyma clade are described as the type strains of Barnettozyma xylosiphila sp. nov. (type strain 13Y206 =NBRC 110202 =InaCC Y726 ; MycoBank MB808598) and Barnettozyma xylosica sp. nov. (type strain 14Y196 =NBRC 111558 =InaCC Y1030 ; MycoBank MB819485). Strain 14Y125 belonging to the Wickerhamomyces clade is described as the type strain of Wickerhamomyces xylosivorus f.a., sp. nov. (type strain 14Y125 =NBRC 111553 =InaCC Y1026 ; MycoBank MB819484).
[Mh] Termos MeSH primário: Filogenia
Saccharomycetales/classificação
Madeira/microbiologia
Xilose/metabolismo
[Mh] Termos MeSH secundário: DNA Fúngico/química
DNA Espaçador Ribossômico/genética
Indonésia
Técnicas de Tipagem Micológica
Fator 1 de Elongação de Peptídeos/genética
Saccharomycetales/genética
Saccharomycetales/isolamento & purificação
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (DNA, Ribosomal Spacer); 0 (Peptide Elongation Factor 1); A1TA934AKO (Xylose)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002233


  3 / 2845 MEDLINE  
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[PMID]:28895516
[Au] Autor:Yurkov AM; Dlauchy D; Péter G
[Ad] Endereço:1​Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Brunswick, Germany.
[Ti] Título:Meyerozyma amylolytica sp. nov. from temperate deciduous trees and the transfer of five Candida species to the genus Meyerozyma.
[So] Source:Int J Syst Evol Microbiol;67(10):3977-3981, 2017 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In the course of two independent studies three yeasts have been isolated from temperate deciduous trees in Hungary and Germany. Analyses of nucleotide sequences of D1/D2 domains of the 26S rRNA gene (LSU) suggested that these strains belong to the Meyerozyma clade in Debaryomycetaceae (Saccharomycetales). The phylogenetic analysis of a concatenated alignment of the ITS region and LSU gene sequences confirmed the placement of the three strains in the Meyerozyma clade close to Candida elateridarum. If mixed in proper combinations, the strains formed one to two hat shaped ascospores in deliquescent asci. In addition to the ascospore formation, the three studied strains differed from Candida elateridarum and other members of the Meyerozyma clade in terms of ribosomal gene sequence and some physiological properties. To accommodate the above-noted strains, we describe the new species as Meyerozyma amylolytica sp. nov. (holotype: DSM 27310 ; ex-type cultures: NCAIM Y.02140 =MUCL 56454 , allotype: NCAIM Y.01955 ; ex-allotype culture: DSM 27468), MB 821663. Additionally, we propose the transfer of five non-ascosporic members of the Meyerozyma clade to the genus Meyerozyma as the following new taxonomic combinations Meyerozyma athensensis f.a., comb. nov. (MB 821664), Meyerozyma carpophila f.a., comb. nov. (MB 821665), Meyerozyma elateridarum f.a., comb. nov. (MB 821666), Meyerozyma neustonensis f.a., comb. nov. (MB 821667), and Meyerozyma smithsonii f.a., comb. nov. (MB 821668).
[Mh] Termos MeSH primário: Candida/classificação
Filogenia
Saccharomycetales/classificação
Árvores/microbiologia
[Mh] Termos MeSH secundário: DNA Fúngico/genética
Alemanha
Hungria
Técnicas de Tipagem Micológica
RNA Ribossômico/genética
Saccharomycetales/genética
Saccharomycetales/isolamento & purificação
Análise de Sequência de DNA
Esporos Fúngicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (RNA, Ribosomal); 0 (RNA, ribosomal, 26S)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002232


  4 / 2845 MEDLINE  
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[PMID]:28884677
[Au] Autor:Morais CG; Batista TM; Kominek J; Borelli BM; Furtado C; Moreira RG; Franco GR; Rosa LH; Fonseca C; Hittinger CT; Lachance MA; Rosa CA
[Ad] Endereço:1​Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
[Ti] Título:Spathaspora boniae sp. nov., a D-xylose-fermenting species in the Candida albicans/Lodderomyces clade.
[So] Source:Int J Syst Evol Microbiol;67(10):3798-3805, 2017 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two yeast isolates producing asci-containing elongate ascospores with curved ends typical of the genus Spathaspora were isolated from rotting wood samples collected in an Atlantic rainforest ecosystem in Brazil. Phylogenetic analysis of the LSU rRNA gene D1/D2 domain sequences demonstrated that the strains represent a new species and placed it next to Candida blackwellae, in a clade that also contains Candida albicans and Candida dubliniensis. Other sequences of the ribosomal gene cluster supported same placementin the same clade, and a phylogenomic analysis placed this new species in an early emerging position relative to the larger C. albicans/Lodderomyces clade. One interpretation is that the genus Spathaspora is, in fact, paraphyletic. In conformity with this view, we propose the novel species Spathaspora boniae sp. nov. to accommodate the isolates. The type strain of Spathaspora boniae sp. nov. is UFMG-CM-Y306 (=CBS 13262 ). The MycoBank number is MB 821297. A detailed analysis of xylose metabolism was conducted for the new species.
[Mh] Termos MeSH primário: Filogenia
Saccharomycetales/classificação
Madeira/microbiologia
Xilose/metabolismo
[Mh] Termos MeSH secundário: Brasil
DNA Fúngico/genética
Fermentação
Genes de RNAr
Técnicas de Tipagem Micológica
RNA Ribossômico 16S/genética
Saccharomycetales/genética
Saccharomycetales/isolamento & purificação
Análise de Sequência de DNA
Esporos Fúngicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (RNA, Ribosomal, 16S); A1TA934AKO (Xylose)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002186


  5 / 2845 MEDLINE  
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[PMID]:28875908
[Au] Autor:Pontes A; Röhl O; Maldonado C; Yurkov AM; Sampaio JP
[Ad] Endereço:1​UCIBIO-REQUIMTE, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.
[Ti] Título:Cryptotrichosporon argae sp. nov., Cryptotrichosporon brontae sp. nov. and Cryptotrichosporon steropae sp. nov., isolated from forest soils.
[So] Source:Int J Syst Evol Microbiol;67(9):3610-3614, 2017 Sep.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Yeast strains belonging to the basidiomycetous genus Cryptotrichosporon were isolated from forest soils in Serra da Arrábida Natural Park in Portugal. Similar to the already-known representatives of this genus, the new isolates formed pigmented colonies of a distinctive pale orange colour. Phylogenetic analyses employing concatenated sequences of the D1/D2 domains of the 26S (large subunit) rRNA gene and the internal transcribed spacer (ITS) region supported the recognition of three novel species: Cryptotrichosporon argae sp. nov. (type strain CM 19T=CBS 14376T=PYCC 7010T=DSM 104550T; MycoBank accession number MB 817168), Cryptotrichosporon brontae sp. nov. (type strain CM 1562T=CBS 14303T=PYCC 7011T=DSM 104551T; MycoBank accession number MB 817077) and Cryptotrichosporon steropae sp. nov. (type strain OR 395T=CBS 14302T=PYCC 7012T=DSM 104552T; MycoBank accession number MB 817078).
[Mh] Termos MeSH primário: Basidiomycota/classificação
Florestas
Filogenia
Microbiologia do Solo
[Mh] Termos MeSH secundário: Basidiomycota/genética
Basidiomycota/isolamento & purificação
DNA Fúngico/genética
DNA Espaçador Ribossômico/genética
Técnicas de Tipagem Micológica
Pigmentação
Portugal
RNA Ribossômico/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (DNA, Ribosomal Spacer); 0 (RNA, Ribosomal); 0 (RNA, ribosomal, 26S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002177


  6 / 2845 MEDLINE  
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[PMID]:28854190
[Au] Autor:Al-Obaid K; Asadzadeh M; Ahmad S; Khan Z
[Ad] Endereço:Microbiology, Department of Medical Laboratories, Al-Amiri Hospital, Sharq, Kuwait.
[Ti] Título:Population structure and molecular genetic characterization of clinical Candida tropicalis isolates from a tertiary-care hospital in Kuwait reveal infections with unique strains.
[So] Source:PLoS One;12(8):e0182292, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Candida tropicalis is a frequently isolated yeast species causing bloodstream, urinary tract and other infections particularly in patients admitted to intensive care units (ICUs) and those requiring prolonged urinary catheterization (UC) or receiving broad-spectrum antibiotics (BSA). This study investigated clinical characteristics and genetic relatedness among C. tropicalis strains isolated from patients at Al-Amiri Hospital in Kuwait. C. tropicalis strains (n = 63) isolated from blood, genito-urinary, respiratory (RT) and digestive (GIT) tracts and wound sites from 54 patients were used. All isolates were phenotypically identified and tested against six antifungal drugs by using Vitek 2 system. Molecular identification was performed by PCR amplification of rDNA. Fingerprinting was achieved by 6-loci-based multilocus sequence typing (MLST) and data were analyzed by BioNumerics software for phylogenetic relationships. Patients mean age was >65 years and >20% patients were hospitalized in ICUs. Most patients had underlying conditions that included UC, BSA, diabetes and RT/GIT abnormalities. Most candiduria cases had UC, ureteric stent or suprapubic catheters. All isolates were identified as C. tropicalis by Vitek 2 and by species-specific PCR. Sixty-two isolates were susceptible to all tested antifungal drugs. MLST identified 59 diploid sequence types (DSTs) including 54 newly-identified DSTs. C. tropicalis isolates from multiple sites of same patient usually belonged to different DSTs. Interestingly, 56 of 57 isolates from 48 patients belonged to unique genotypes. Only six isolates from six patients belonged to three DSTs (clusters), however, C. tropicalis strains in each cluster were isolated >3 months apart. Our data show diverse origins of C. tropicalis infections in Kuwait as most isolates were unique strains. There was no obvious correlation between cluster isolates with time of isolation and/or hospital ward of their origin. This study presents the first MLST analysis of C. tropicalis isolates from Middle East and may be useful for studying genetic relationships among global C. tropicalis strains.
[Mh] Termos MeSH primário: Candida tropicalis/genética
Candidíase/microbiologia
[Mh] Termos MeSH secundário: Antifúngicos/farmacologia
Candida tropicalis/classificação
Candida tropicalis/efeitos dos fármacos
Candida tropicalis/isolamento & purificação
Candidíase/diagnóstico
Candidíase/tratamento farmacológico
Candidíase/epidemiologia
Farmacorresistência Fúngica
Variação Genética
Genótipo
Seres Humanos
Kuweit/epidemiologia
Testes de Sensibilidade Microbiana
Tipagem de Sequências Multilocus
Técnicas de Tipagem Micológica
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170831
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182292


  7 / 2845 MEDLINE  
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[PMID]:28840804
[Au] Autor:Gao WL; Liu TT; Zheng J; Hui FL
[Ad] Endereço:1​School of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, PR China.
[Ti] Título:Kodamaea neixiangensis f.a., sp. nov. and Kodamaea jinghongensis f.a., sp. nov., two yeast species isolated from rotting wood.
[So] Source:Int J Syst Evol Microbiol;67(9):3358-3362, 2017 Sep.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Seven strains representing two novel yeast species were isolated from rotting wood in Henan and Yunnan Provinces, PR China. The results of phylogenetic analysis based on the D1/D2 domains of the large subunit (LSU) rRNA gene revealed that these two species are members of the genus Kodamaea, although the formation of ascospores was not observed. Kodamaea neixiangensis f.a., sp. nov. (type strain NYNU 167139T=CICC 33170T=CBS 14699T) formed a clade with Candida kaohsiungensis and Candida hsintzibuensis, from which it differed by 10-16 substitutions in the D1/D2 domain. The ITS sequences of K. neixiangensis sp. nov. differed by 27 substitutions from those of the type strain of C. kaohsiungensis. The most closely related species with a validly published name to Kodamaea jinghongensis f.a., sp. nov. (type strain NYNU 167162T=CICC 33171T=CBS 14700T) was Candida fukazawae, but this differed by 14 substitutions in the D1/D2 domain and by 15 substitutions in the ITS region.
[Mh] Termos MeSH primário: Filogenia
Saccharomycetales/classificação
Madeira/microbiologia
[Mh] Termos MeSH secundário: China
DNA Fúngico/genética
DNA Espaçador Ribossômico/genética
Técnicas de Tipagem Micológica
RNA Ribossômico/genética
Saccharomycetales/genética
Saccharomycetales/isolamento & purificação
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (DNA, Ribosomal Spacer); 0 (RNA, Ribosomal)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170826
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002117


  8 / 2845 MEDLINE  
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[PMID]:28820107
[Au] Autor:Lu YF; Wang M; Zheng J; Hui FL
[Ad] Endereço:School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, PR China.
[Ti] Título:Ogataea neixiangensis sp. nov. and Ogataea paraovalis f.a., sp. nov., two methanol-assimilating yeast species isolated from rotting wood.
[So] Source:Int J Syst Evol Microbiol;67(8):3038-3042, 2017 Aug.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Four yeast strains were isolated from rotting wood samples collected in the Baotianman Nature Reserve in Henan Province, central China. The sequences of the D1/D2 domains of the large subunit rRNA gene and the internal transcribed spacer regions showed that these four strains represent two different undescribed yeast species belonging to the Ogataea clade. Ogataea neixiangensis sp. nov. produces two to four hat-shaped ascospores per ascus, and its closest relative among recognized species is Candida nitratophila. Ogataea paraovalis f.a., sp. nov. is closely related to Candida ovalis but the formation of ascospores was not observed on various sporulation media. The type strain of O. neixiangensis sp. nov. (MycoBank number MB 820697) is NYNU 16951T (=CICC 33166T=CBS 14695T), and the type strain of O. paraovalis f.a., sp. nov. (MycoBank number MB 820698) is NYNU 167106T (=CICC 33168T=CBS 14697T).
[Mh] Termos MeSH primário: Metanol/metabolismo
Filogenia
Saccharomycetales/classificação
Madeira/microbiologia
[Mh] Termos MeSH secundário: China
DNA Fúngico/genética
Técnicas de Tipagem Micológica
RNA Ribossômico/genética
RNA Ribossômico 16S/genética
Saccharomycetales/genética
Saccharomycetales/isolamento & purificação
Análise de Sequência de DNA
Esporos Fúngicos/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (RNA, Ribosomal); 0 (RNA, Ribosomal, 16S); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170819
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002075


  9 / 2845 MEDLINE  
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[PMID]:28820103
[Au] Autor:Yamazaki A; Yanagiba M; Naganuma T
[Ad] Endereço:1​NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
[Ti] Título:Two novel Lipomycetaceous yeast species, Lipomyces okinawensis sp. nov. and Lipomyces yamanashiensis f.a., sp. nov., isolated from soil in the Okinawa and Yamanashi prefectures, Japan.
[So] Source:Int J Syst Evol Microbiol;67(8):2941-2946, 2017 Aug.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Four novel Lipomyces strains were isolated from soil collected in the Okinawa and Yamanashi prefectures, Japan. Based on their morphological and biochemical characteristics, along with sequence typing using the D1/D2 domain of the LSU rRNA, internal transcribed spacer (ITS) region including 5.8S rRNA, and translation elongation factor 1 alpha gene (EF-1α), the four strains were shown to represent two novel species of the genus Lipomyces, described as Lipomyces okinawensis sp. nov. (type strain No.3-a(35)T=NBRC 110620T=CBS 14747T) and Lipomyces yamanashiensis f.a., sp. nov. (type strain No.313T=NBRC 110621T=CBS 14748T).
[Mh] Termos MeSH primário: Lipomyces/classificação
Filogenia
Microbiologia do Solo
[Mh] Termos MeSH secundário: DNA Fúngico/genética
DNA Espaçador Ribossômico/genética
Genes Fúngicos
Japão
Lipomyces/genética
Lipomyces/isolamento & purificação
Técnicas de Tipagem Micológica
Fator 1 de Elongação de Peptídeos/genética
RNA Ribossômico/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (DNA, Ribosomal Spacer); 0 (Peptide Elongation Factor 1); 0 (RNA, Ribosomal)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170819
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002050


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[PMID]:28771588
[Au] Autor:Dögen A; Metin B; Ilkit M; de Hoog GS; Heitman J
[Ad] Endereço:Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Mersin, Mersin, Turkey.
[Ti] Título:MTL genotypes, phenotypic switching, and susceptibility profiles of Candida parapsilosis species group compared to Lodderomyces elongisporus.
[So] Source:PLoS One;12(8):e0182653, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Reference isolates of Candida parapsilosis (n = 8), Candida metapsilosis (n = 6), Candida orthopsilosis (n = 7), and Lodderomyces elongisporus (n = 11) were analyzed to gain insight into their pathobiology and virulence mechanisms. Initial evaluation using BBL Chromagar Candida medium misidentified L. elongisporus isolates as C. albicans. Polymerase chain reaction analysis of isolate MTL idiomorphs revealed that all C. parapsilosis isolates were MTLa homozygous and no MTL α1, α2, a1, or a2 gene was detected in L. elongisporus isolates. For C. orthopsilosis, two isolates were MTLa homozygous and five were MTL-heterozygous. Similarly, one C. metapsilosis isolate was MTLα homozygous whereas five were MTL-heterozygous. Isolate phenotypic switching analysis revealed potential phenotypic switching in the MTLα homozygous C. metapsilosis isolate, resulting in concomitant elongated cell formation. Minimum inhibitory concentrations of fluconazole (FLC) and FK506, alone or in combination, were determined by checkerboard assay, with data analyzed using the fractional inhibitory concentration index model. Synergistic or additive effects of these compounds were commonly observed in C. parapsilosis and L. elongisporus isolates. No killer activity was observed in the studied isolates, as determined phenotypically. No significant difference in virulence was seen for the four species in a Galleria mellonella model (P > 0.05). In conclusion, our results demonstrated phenotypic switching of C. metapsilosis CBS 2315 and that FLC and FK506 represent a promising drug combination against C. parapsilosis and L. elongisporus. The findings of the present study contribute to our understanding of the biology, diagnosis, and new possible treatments of the C. parapsilosis species group and L. elongisporus.
[Mh] Termos MeSH primário: Antifúngicos/farmacologia
Candida/classificação
Genes Fúngicos Tipo Acasalamento
Saccharomycetales/classificação
[Mh] Termos MeSH secundário: Candida/efeitos dos fármacos
Candida/genética
Testes de Sensibilidade Microbiana
Técnicas de Tipagem Micológica
Polimorfismo de Fragmento de Restrição
Saccharomycetales/efeitos dos fármacos
Saccharomycetales/genética
Análise de Sequência de DNA
Fatores de Virulência/genética
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Virulence Factors)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182653



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