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[PMID]:29231012
[Au] Autor:Miao CY; Xu L; Wang N; Zhang M; Li YS; Lü JX
[Ad] Endereço:Dongcheng Branch of Beijing Public Security Bureau, Beijing 100061, China.
[Ti] Título:[Progress in Application of Measuring Skeleton by CT in Forensic Anthropology Research].
[So] Source:Fa Yi Xue Za Zhi;33(1):58-61, 2017 Feb.
[Is] ISSN:1004-5619
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:Individual identification by measuring the human skeleton is an important research in the field of forensic anthropology. Computed tomography (CT) technology can provide high-resolution image of skeleton. Skeleton image can be reformed by software in the post-processing workstation. Different skeleton measurement indexes of anthropology, such as diameter, angle, area and volume, can be measured on section and reformative images. Measurement process is barely affected by human factors. This paper reviews the literatures at home and abroad about the application of measuring skeleton by CT in forensic anthropology research for individual identification in four aspects, including sex determination, height infer, facial soft tissue thickness measurement and age estimation. The major technology and the application of CT in forensic anthropology research are compared and discussed, respectively.
[Mh] Termos MeSH primário: Determinação da Idade pelo Esqueleto
Osso e Ossos/diagnóstico por imagem
Antropologia Forense/métodos
Análise para Determinação do Sexo
Tomografia Computadorizada por Raios X/métodos
[Mh] Termos MeSH secundário: Antropologia Forense/tendências
Seres Humanos
Software
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.3969/j.issn.1004-5619.2017.01.014


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[PMID]:27771467
[Au] Autor:Juras A; Chylenski M; Krenz-Niedbala M; Malmström H; Ehler E; Pospieszny L; Lukasik S; Bednarczyk J; Piontek J; Jakobsson M; Dabert M
[Ad] Endereço:Department of Human Evolutionary Biology, Institute of Anthropology, Faculty of Biology, Adam Mickiewicz University in Poznan, Umultowska 89, 61-614 Poznan, Poland. Electronic address: annaj@amu.edu.pl.
[Ti] Título:Investigating kinship of Neolithic post-LBK human remains from Krusza Zamkowa, Poland using ancient DNA.
[So] Source:Forensic Sci Int Genet;26:30-39, 2017 01.
[Is] ISSN:1878-0326
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:We applied an interdisciplinary approach to investigate kinship patterns and funerary practices during the middle Neolithic. Genetic studies, radiocarbon dating, and taphonomic analyses were used to examine two grave clusters from Krusza Zamkowa, Poland. To reconstruct kinship and determine biological sex, we extracted DNA from bones and teeth, analyzed mitochondrial genomes and nuclear SNPs using the HID-Ion AmpliSeq™ Identity panel generated on Illumina and Ion Torrent platforms, respectively. We further dated the material (AMS C) and to exclude aquatic radiocarbon reservoir effects, measures of carbon and nitrogen stable isotopes for diet reconstruction were used. We found distinct mitochondrial genomes belonging to haplogroups U5b2a1a, K1c and H3d in the first grave cluster, and excluded maternal kin patterns among the three analyzed individuals. In the second grave cluster one individual belonged to K1a4. However, we could not affiliate the second individual to a certain haplogroup due to the fragmented state of the mitochondrial genome. Although the individuals from the second grave cluster differ at position 6643, we believe that more data is needed to fully resolve this issue. We retrieved between 26 and 77 autosomal SNPs from three of the individuals. Based on kinship estimations, taking into account the allelic dropout distribution, we could not exclude first degree kin relation between the two individuals from the second grave cluster. We could, however, exclude a first degree kinship between these two individuals and an individual from the first grave cluster. Presumably, not only biological kinship, but also social relations played an important role in the funerary practice during this time period. We further conclude that the HID-Ion AmpliSeq™ Identity Panel may prove useful for first degree kin relation studies for samples with good DNA preservation, and that mitochondrial genome capture enrichment is a powerful tool for excluding direct maternal relationship in ancient individuals.
[Mh] Termos MeSH primário: DNA Antigo
DNA Mitocondrial/genética
Linhagem
[Mh] Termos MeSH secundário: Osso e Ossos/química
Isótopos de Carbono
Feminino
Genótipo
Sequenciamento de Nucleotídeos em Larga Escala
Seres Humanos
Masculino
Isótopos de Nitrogênio
Polônia
Reação em Cadeia da Polimerase
Polimorfismo de Nucleotídeo Único
Análise para Determinação do Sexo
Dente/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Carbon Isotopes); 0 (DNA, Ancient); 0 (DNA, Mitochondrial); 0 (Nitrogen Isotopes)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161107
[St] Status:MEDLINE


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[PMID]:28780443
[Au] Autor:Saeedfar K; Heng LY; Chiang CP
[Ad] Endereço:School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Selangor, Malaysia; Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, 1417466191 Tehran, Iran. Electronic address: kasra.saeedfar@ut.ac.ir.
[Ti] Título:A DNA biosensor based on gold nanoparticle decorated on carboxylated multi-walled carbon nanotubes for gender determination of Arowana fish.
[So] Source:Bioelectrochemistry;118:106-113, 2017 Dec.
[Is] ISSN:1878-562X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Multi-wall carbon nanotubes (MWCNTs) were modified to design a new DNA biosensor. Functionalized MWCNTs were equipped with gold nanoparticles (GNPs) (~15nm) (GNP-MWCNTCOOH) to construct DNA biosensors based on carbon-paste screen-printed (SPE) electrodes. GNP attachment onto functionalized MWCNTs was carried out by microwave irradiation and was confirmed by spectroscopic studies and surface analysis. DNA biosensors based on differential pulse voltammetry (DPV) were constructed by immobilizing thiolated single-stranded DNA probes onto GNP-MWCNTCOOH. Ruthenium (III) chloride hexaammoniate [Ru(NH ) ,2Cl ] (RuHex) was used as hybridization redox indicator. RuHex and MWCNT interaction was low in compared to other organic redox hybridization indicators. The linear response range for DNA determination was 1×10 to 1×10 M with a lower detection limit of 1.55×10 M. Thus, the attachment of GNPs onto functionalized MWCNTs yielded sensitive DNA biosensor with low detection limit and stability more than 30days. Constructed electrode was used to determine gender of arowana fish.
[Mh] Termos MeSH primário: Técnicas Biossensoriais/métodos
DNA/análise
Peixes
Ouro/química
Nanopartículas Metálicas/química
Nanotubos de Carbono/química
Análise para Determinação do Sexo/métodos
[Mh] Termos MeSH secundário: Animais
Técnicas Biossensoriais/instrumentação
Ácidos Carboxílicos/química
DNA/química
DNA de Cadeia Simples/análise
DNA de Cadeia Simples/química
Eletrodos
Feminino
Concentração de Íons de Hidrogênio
Masculino
Hibridização de Ácido Nucleico
Sonicação
Fatores de Tempo
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carboxylic Acids); 0 (DNA, Single-Stranded); 0 (Nanotubes, Carbon); 7440-57-5 (Gold); 9007-49-2 (DNA)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170807
[St] Status:MEDLINE


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[PMID]:28664631
[Au] Autor:Valadan R; Nejatollahi F; Ehsani-Nori H; Habibi H; Amini H; Aliabadian M
[Ad] Endereço:Molecular and Cell Biology Research Center, Department of Immunology, Mazandaran University of Medical Sciences, Sari, Iran.
[Ti] Título:Avian gametologs as molecular tags for sex identification in birds of prey of Iran.
[So] Source:Zoo Biol;36(4):289-293, 2017 Jul.
[Is] ISSN:1098-2361
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Global environmental change and rapid destruction of natural habitats necessitate the conservation of endangered and threatened birds of prey. Recently, molecular sex identification methods based on amplification of introns of chromodomain-helicase DNA binding protein1 (CHD1) have provided valuable tools for ecological study and conservation breeding programs of birds. These methods employ a primer pair flanking an intron which varies considerably in length between the avian gametologs CHD1Z and CHD1W. Herein, we test the applicability of CHD1Z and CHD1W as universal tags for molecular sex identification in birds of prey of Iran. We showed successful sex identification in 22 species of birds of prey using feathers as the source of DNA. The results suggest that the regions of CHD1W and CHD1Z amplified in this study are conserved among most of Falconiformes, enabling accurate sex identification in birds of prey.
[Mh] Termos MeSH primário: Proteínas Aviárias/metabolismo
Proteínas de Ligação a DNA/metabolismo
Aves Predatórias/fisiologia
Análise para Determinação do Sexo/veterinária
[Mh] Termos MeSH secundário: Animais
Proteínas Aviárias/genética
Proteínas de Ligação a DNA/genética
Feminino
Regulação da Expressão Gênica
Masculino
Análise para Determinação do Sexo/métodos
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Avian Proteins); 0 (DNA-Binding Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170701
[St] Status:MEDLINE
[do] DOI:10.1002/zoo.21363


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[PMID]:28628900
[Au] Autor:Ristow PG; Barnes N; Murphy GP; Brown H; Cloete KW; D'Amato ME
[Ad] Endereço:Forensic DNA Laboratory, Department of Biotechnology, University of the Western Cape, Bellville, 7535, South Africa; Centre for GeoGenetics, Natural History Museum of Denmark, University of Copenhagen, Øster Voldgade 5-7, 1350, Copenhagen, Denmark.
[Ti] Título:Evaluation of the InnoTyper 21 genotyping kit in multi-ethnic populations.
[So] Source:Forensic Sci Int Genet;30:43-50, 2017 Sep.
[Is] ISSN:1878-0326
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:We report the findings of the evaluation of the InnoTyper 21 genotyping kit for the use of human identification (HID) and paternity testing in South Africa. This novel forensic kit evaluates 20 retrotransposable elements (AC4027, MLS26, ALU79712, NBC216, NBC106, RG148, NBC13, AC2265, MLS09, AC1141, TARBP, AC2305, HS4.69, NBC51, ACA1766, NBC120, NBC10, NBC102, SB19.12 and NBC148) and the Amelogenin locus for sex determination. The evaluation of the genotyping performance showed no significant spectral pull-up for peak heights between 100 and 30,000 RFUs. All loci presented biallelic patterns except the triallelic RG148 locus resulting from a variant insertion allele, named RG148I-1, observed exclusively in the Bantu. The InnoTyper 21 kit was found to be highly discriminatory between the 507 unrelated individuals of the Afrikaaner, Asian Indian, Coloured, amaXhosa and amaZulu groups. The HID parameters: the CPD ranged between 0.99999987 and 0.9999999845, and the CMP between 1.0335×10 and 1.5506×10 . The paternity parameters: the CPI ranged between 0.0202 and 0.3177, and the CPE between 0.9161 and 0.9749. There were no significant signs of deviations from HWE or linkage disequilibrium (LD) after applying a Bonferroni correction. This kit also showed minor levels of population structure which could differentiate between the African and non-African population groups. Finally, in challenging casework with severely degraded biological material, the InnoTyper 21 genotyping kit was compatible with GlobalFiler and Investigator DIPplex to increase the HID parameters.
[Mh] Termos MeSH primário: Impressões Digitais de DNA/instrumentação
Grupos Étnicos/genética
Genética Populacional
Genótipo
[Mh] Termos MeSH secundário: Alelos
Amelogenina/genética
Análise Discriminante
Seres Humanos
Repetições de Microssatélites
Análise para Determinação do Sexo
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amelogenin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171011
[Lr] Data última revisão:
171011
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE


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[PMID]:28582396
[Au] Autor:Oike A; Kodama M; Yasumasu S; Yamamoto T; Nakamura Y; Ito E; Nakamura M
[Ad] Endereço:Department of Biology, Faculty of Education and Integrated Arts and Sciences, Waseda University, Shinjuku-ku, Tokyo, Japan.
[Ti] Título:Participation of androgen and its receptor in sex determination of an amphibian species.
[So] Source:PLoS One;12(6):e0178067, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: In the Japanese frog Rana (R.) rugosa the androgen receptor (AR) gene on the W chromosome (W-AR) is barely expressed. Previously we showed that incomplete female-to-male sex-reversal occurred in Z-AR transgenic female frogs. To date, however, there is no report showing that AR with androgens can determine genetically programed male sex fate in any vertebrate species. Here, we examined whether AR together with androgens functions as a sex determinant in an amphibian species. METHODS: To examine whether complete female-to-male sex-reversal occurs in R. rugosa frogs, we produced AR-transgenic (Tg) and -knockdown (KD) female R. rugosa frogs by the I-SceI meganuclease-mediated gene trap and CRISPR/Cas9 system, respectively. AR-Tg and -KD tadpoles were reared in water containing testosterone (T) at 0 to 7.1 ng/ml. Frozen sections were prepared from the gonads of metamorphosed frogs and immunostained for laminin, Vasa, Pat1a, CYP17 and AR. We also employed PCR analysis to examine Dmrt1, Pat1a and CYP17 expression in the gonads of KD and placebo-KD female frogs. RESULTS: Complete female-to-male sex-reversal occurred in the AR-Tg ZW female frogs when a low dosage of T was supplied in the rearing water of tadpoles. However, no sex-reversal was observed in AR-KD ZW female frogs when the gonads were treated with dosages of T high enough to induce complete female-to-male sex-reversal even in wild type frogs. DISCUSSION: These results suggest that AR with its androgen ligand functions as a male sex-determinant in the ZW type R. rugosa frogs.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica no Desenvolvimento
Ranidae/genética
Receptores Androgênicos/genética
Cromossomos Sexuais/efeitos dos fármacos
Processos de Determinação Sexual
Testosterona/farmacologia
[Mh] Termos MeSH secundário: Proteínas de Anfíbios/genética
Proteínas de Anfíbios/metabolismo
Animais
Animais Geneticamente Modificados
Sequência de Bases
Sistemas CRISPR-Cas
RNA Helicases DEAD-box/genética
RNA Helicases DEAD-box/metabolismo
Desoxirribonucleases de Sítio Específico do Tipo II/genética
Desoxirribonucleases de Sítio Específico do Tipo II/metabolismo
Feminino
Edição de Genes
Técnicas de Silenciamento de Genes
Laminina/genética
Laminina/metabolismo
Larva/efeitos dos fármacos
Larva/genética
Larva/crescimento & desenvolvimento
Larva/metabolismo
Masculino
Proteínas Serina-Treonina Quinases/genética
Proteínas Serina-Treonina Quinases/metabolismo
Ranidae/crescimento & desenvolvimento
Ranidae/metabolismo
Receptores Androgênicos/deficiência
Proteínas de Saccharomyces cerevisiae/genética
Proteínas de Saccharomyces cerevisiae/metabolismo
Cromossomos Sexuais/química
Cromossomos Sexuais/metabolismo
Análise para Determinação do Sexo
Esteroide 17-alfa-Hidroxilase/genética
Esteroide 17-alfa-Hidroxilase/metabolismo
Testosterona/metabolismo
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amphibian Proteins); 0 (DMRT1 protein); 0 (Laminin); 0 (Receptors, Androgen); 0 (Saccharomyces cerevisiae Proteins); 0 (Transcription Factors); 3XMK78S47O (Testosterone); EC 1.14.14.19 (Steroid 17-alpha-Hydroxylase); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); EC 3.1.21.- (SCEI protein, S cerevisiae); EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific); EC 3.6.4.13 (DEAD-box RNA Helicases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170606
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178067


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[PMID]:28534314
[Au] Autor:Bakker TCM; Giger T; Frommen JG; Largiadèr CR
[Ad] Endereço:Institute for Evolutionary Biology and Ecology, University of Bonn, An der Immenburg 1, 53121, Bonn, Germany. tbakker@evolution.uni-bonn.de.
[Ti] Título:Rapid molecular sexing of three-spined sticklebacks, Gasterosteus aculeatus L., based on large Y-chromosomal insertions.
[So] Source:J Appl Genet;58(3):401-407, 2017 Aug.
[Is] ISSN:2190-3883
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:There is a need for rapid and reliable molecular sexing of three-spined sticklebacks, Gasterosteus aculeatus, the supermodel species for evolutionary biology. A DNA region at the 5' end of the sex-linked microsatellite Gac4202 was sequenced for the X chromosome of six females and the Y chromosome of five males from three populations. The Y chromosome contained two large insertions, which did not recombine with the phenotype of sex in a cross of 322 individuals. Genetic variation (SNPs and indels) within the insertions was smaller than on flanking DNA sequences. Three molecular PCR-based sex tests were developed, in which the first, the second or both insertions were covered. In five European populations (from DE, CH, NL, GB) of three-spined sticklebacks, tests with both insertions combined showed two clearly separated bands on agarose minigels in males and one band in females. The tests with the separate insertions gave similar results. Thus, the new molecular sexing method gave rapid and reliable results for sexing three-spined sticklebacks and is an improvement and/or alternative to existing methods.
[Mh] Termos MeSH primário: Mutação INDEL
Análise para Determinação do Sexo/métodos
Smegmamorpha/genética
Cromossomo Y/genética
[Mh] Termos MeSH secundário: Animais
Feminino
Masculino
Repetições de Microssatélites
Fenótipo
Polimorfismo de Nucleotídeo Único
Análise para Determinação do Sexo/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170524
[St] Status:MEDLINE
[do] DOI:10.1007/s13353-017-0399-0


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[PMID]:28424205
[Au] Autor:Sugiyama A; Shichi K; Masaki T; Hubbell SP
[Ad] Endereço:Department of Ecology and Evolutionary Biology, University of California, Los Angeles, California 90095 USA anna.sugiyama@yale.edu.
[Ti] Título:The use of soil pollen to determine the sex of overhead individuals of a temperate dioecious shrub.
[So] Source:Am J Bot;104(4):632-638, 2017 Apr.
[Is] ISSN:1537-2197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PREMISE OF THE STUDY: In dioecious species, determining the sex of individual plants from one-time phenological observations is rarely feasible when some individuals capable of reproducing are not flowering or fruiting at the time of observation. Currently, sexing those individuals requires long-term phenological data on individuals and populations, but such data are rarely available or feasible to collect. We tested the hypothesis that differences in soil pollen concentrations beneath the crowns of female and male plants would exist and be sufficient to reliably determine the sex of the individual plant overhead in a dioecious species. We predicted that soil pollen concentrations beneath male plants would be significantly higher than beneath female plants because only males produce pollen and pollen should accumulate in the soil underneath the male plants over repeated flowering events. METHODS: We collected samples from surface soil under both sexes of the insect-pollinated dioecious shrub, (Garryaceae). KEY RESULTS: Pollen grains were present in surface soil in both Oe and A horizons, and mean pollen concentration under males was significantly higher than under females. Pollen concentrations beneath males were positively correlated with male plant height, potentially reflecting greater pollen production by larger individuals. CONCLUSIONS: Considering the small plant size and relatively low pollen production of , this method may hold promise for sexing other dioecious species in the absence of direct phenological data. Our phenology-free and relatively low-cost method for sexing dioecious plants may be especially useful in tropical forests where many species are dioecious.
[Mh] Termos MeSH primário: Magnoliopsida
Pólen
Solo
[Mh] Termos MeSH secundário: Análise para Determinação do Sexo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170421
[St] Status:MEDLINE
[do] DOI:10.3732/ajb.1600407


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[PMID]:28391819
[Au] Autor:Akkoç B; Arslan A; Kök H
[Ad] Endereço:Department of Computer Engineering, Faculty of Engineering, Selçuk University, Aleaddin Keykubad Campus, Konya 42075, Turkey. Electronic address: betulakkoc@selcuk.edu.tr.
[Ti] Título:Automatic gender determination from 3D digital maxillary tooth plaster models based on the random forest algorithm and discrete cosine transform.
[So] Source:Comput Methods Programs Biomed;143:59-65, 2017 May.
[Is] ISSN:1872-7565
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND OBJECTIVE: One of the first stages in the identification of an individual is gender determination. Through gender determination, the search spectrum can be reduced. In disasters such as accidents or fires, which can render identification somewhat difficult, durable teeth are an important source for identification. This study proposes a smart system that can automatically determine gender using 3D digital maxillary tooth plaster models. METHODS: The study group was composed of 40 Turkish individuals (20 female, 20 male) between the ages of 21 and 24. Using the iterative closest point (ICP) algorithm, tooth models were aligned, and after the segmentation process, models were transformed into depth images. The local discrete cosine transform (DCT) was used in the process of feature extraction, and the random forest (RF) algorithm was used for the process of classification. RESULTS: Classification was performed using 30 different seeds for random generator values and 10-fold cross-validation. A value of 85.166% was obtained for average classification accuracy (CA) and a value of 91.75% for the area under the ROC curve (AUC). CONCLUSIONS: A multi-disciplinary study is performed here that includes computer sciences, medicine and dentistry. A smart system is proposed for the determination of gender from 3D digital models of maxillary tooth plaster models. This study has the capacity to extend the field of gender determination from teeth.
[Mh] Termos MeSH primário: Processamento de Imagem Assistida por Computador/métodos
Imagem Tridimensional/métodos
Maxila/diagnóstico por imagem
Análise para Determinação do Sexo/métodos
Tomografia Computadorizada por Raios X/métodos
Dente/diagnóstico por imagem
[Mh] Termos MeSH secundário: Adulto
Algoritmos
Área Sob a Curva
Simulação por Computador
Modelos Dentários
Feminino
Seres Humanos
Masculino
Reconhecimento Automatizado de Padrão
Curva ROC
Reprodutibilidade dos Testes
Fatores Sexuais
Turquia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170411
[St] Status:MEDLINE


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[PMID]:28273159
[Au] Autor:Alibhai S; Jewell Z; Evans J
[Ad] Endereço:Nicholas School of the Environment, Duke University, Durham, North Carolina, United States of America.
[Ti] Título:The challenge of monitoring elusive large carnivores: An accurate and cost-effective tool to identify and sex pumas (Puma concolor) from footprints.
[So] Source:PLoS One;12(3):e0172065, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Acquiring reliable data on large felid populations is crucial for effective conservation and management. However, large felids, typically solitary, elusive and nocturnal, are difficult to survey. Tagging and following individuals with VHF or GPS technology is the standard approach, but costs are high and these methodologies can compromise animal welfare. Such limitations can restrict the use of these techniques at population or landscape levels. In this paper we describe a robust technique to identify and sex individual pumas from footprints. We used a standardized image collection protocol to collect a reference database of 535 footprints from 35 captive pumas over 10 facilities; 19 females (300 footprints) and 16 males (235 footprints), ranging in age from 1-20 yrs. Images were processed in JMP data visualization software, generating one hundred and twenty three measurements from each footprint. Data were analyzed using a customized model based on a pairwise trail comparison using robust cross-validated discriminant analysis with a Ward's clustering method. Classification accuracy was consistently > 90% for individuals, and for the correct classification of footprints within trails, and > 99% for sex classification. The technique has the potential to greatly augment the methods available for studying puma and other elusive felids, and is amenable to both citizen-science and opportunistic/local community data collection efforts, particularly as the data collection protocol is inexpensive and intuitive.
[Mh] Termos MeSH primário: Puma/fisiologia
Análise para Determinação do Sexo/economia
Análise para Determinação do Sexo/métodos
[Mh] Termos MeSH secundário: Algoritmos
Animais
Análise por Conglomerados
Feminino

Masculino
Fotografia
Densidade Demográfica
Vigilância da População
Puma/anatomia & histologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170830
[Lr] Data última revisão:
170830
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170309
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0172065



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