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[PMID]:29458687
[Au] Autor:Tracz DM; Tober AD; Antonation KS; Corbett CR
[Ad] Endereço:1​National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba, R3E 3R2, Canada.
[Ti] Título:MALDI-TOF mass spectrometry and high-consequence bacteria: safety and stability of biothreat bacterial sample testing in clinical diagnostic laboratories.
[So] Source:J Med Microbiol;67(3):341-346, 2018 Mar.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We considered the application of MALDI-TOF mass spectrometry for BSL-3 bacterial diagnostics, with a focus on the biosafety of live-culture direct-colony testing and the stability of stored extracts. Biosafety level 2 (BSL-2) bacterial species were used as surrogates for BSL-3 high-consequence pathogens in all live-culture MALDI-TOF experiments. Viable BSL-2 bacteria were isolated from MALDI-TOF mass spectrometry target plates after 'direct-colony' and 'on-plate' extraction testing, suggesting that the matrix chemicals alone cannot be considered sufficient to inactivate bacterial culture and spores in all samples. Sampling of the instrument interior after direct-colony analysis did not recover viable organisms, suggesting that any potential risks to the laboratory technician are associated with preparation of the MALDI-TOF target plate before or after testing. Secondly, a long-term stability study (3 years) of stored MALDI-TOF extracts showed that match scores can decrease below the threshold for reliable species identification (<1.7), which has implications for proficiency test panel item storage and distribution.
[Mh] Termos MeSH primário: Infecções Bacterianas/diagnóstico
Técnicas Bacteriológicas
Armas Biológicas
Técnicas de Laboratório Clínico/métodos
Contenção de Riscos Biológicos
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
[Mh] Termos MeSH secundário: Bactérias/isolamento & purificação
Infecções Bacterianas/microbiologia
Técnicas Bacteriológicas/instrumentação
Técnicas de Laboratório Clínico/instrumentação
Seres Humanos
Manejo de Espécimes/efeitos adversos
Manejo de Espécimes/instrumentação
Manejo de Espécimes/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biological Warfare Agents)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000695


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[PMID]:28457785
[Au] Autor:Glushakova LG; Alto BW; Kim MS; Bradley A; Yaren O; Benner SA
[Ad] Endereço:Firebird Biomolecular Sciences LLC,13709 Progress Blvd, Box 17, Alachua, FL 32615, United States.
[Ti] Título:Detection of chikungunya viral RNA in mosquito bodies on cationic (Q) paper based on innovations in synthetic biology.
[So] Source:J Virol Methods;246:104-111, 2017 Aug.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Chikungunya virus (CHIKV) represents a growing and global concern for public health that needs inexpensive and convenient methods to collect mosquitoes as potential carriers so that they can be preserved, stored and transported for later and/or remote analysis. Reported here is a cellulose-based paper, derivatized with quaternary ammonium groups ("Q-paper") that meets these needs. In a series of tests, infected mosquito bodies were squashed directly on Q-paper. Aqueous ammonia was then added on the mosquito bodies to release viral RNA that adsorbed on the cationic surface via electrostatic interactions. The samples were then stored (frozen) or transported. For analysis, the CHIKV nucleic acids were eluted from the Q-paper and PCR amplified in a workflow, previously developed, that also exploited two nucleic acid innovations, ("artificially expanded genetic information systems", AEGIS, and "self-avoiding molecular recognition systems", SAMRS). The amplicons were then analyzed by a Luminex hybridization assay. This procedure detected CHIKV RNA, if present, in each infected mosquito sample, but not in non-infected counterparts or ddH O samples washes, with testing one week or ten months after sample collection.
[Mh] Termos MeSH primário: Aedes/virologia
Vírus Chikungunya/genética
Vírus Chikungunya/isolamento & purificação
Papel
RNA Viral/isolamento & purificação
Biologia Sintética/métodos
[Mh] Termos MeSH secundário: Animais
Hibridização de Ácido Nucleico/métodos
Preservação Biológica
Compostos de Amônio Quaternário/química
RNA Viral/genética
Manejo de Espécimes/instrumentação
Manejo de Espécimes/métodos
Biologia Sintética/instrumentação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Quaternary Ammonium Compounds); 0 (RNA, Viral)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


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[PMID]:28450173
[Au] Autor:Chandler JC; Schaeffer JW; Davidson M; Magzamen SL; Pérez-Méndez A; Reynolds SJ; Goodridge LD; Volckens J; Franklin AB; Shriner SA; Bisha B
[Ad] Endereço:National Wildlife Research Center, Wildlife Services, Animal and Plant Health Inspection Service, United States Department of Agriculture, Fort Collins, CO, USA.
[Ti] Título:A method for the improved detection of aerosolized influenza viruses and the male-specific (F+) RNA coliphage MS2.
[So] Source:J Virol Methods;246:38-41, 2017 Aug.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The detection of aerosolized viruses can serve as an important surveillance and control tool in agriculture, human health, and environmental settings. Here, we adapted an anion exchange resin-based method, initially developed to concentrate negatively charged viruses from water, to liquid impingement-based bioaerosol sampling. In this method, aerosolized viruses are collected in a 20ml liquid sample contained within widely used impingers, BioSamplers (SKC Inc., Eighty Four, PA), and further concentrated via adsorption to an anion exchange resin that is suspended within this liquid. Viral nucleic acids are then extracted from the resin to facilitate molecular analyses through a reduction in the effective sample volume. For this study, various quantities of two negatively charged viruses, type A and type B influenza viruses (FluMist Quadrivalent vaccine) and the male-specific (F+) RNA coliphage MS2 (MS2), were nebulized into a custom-built bioaerosolization chamber, and sampled using BioSamplers with and without anion exchange resin. Compared to direct testing of the BioSampler liquid, detection was improved by 6.77× and 3.33× for type A and type B influenza viruses, respectively, by using the anion exchange resin. For MS2, the anion exchange resin method allowed for an average improvement in detection of 8.26×.
[Mh] Termos MeSH primário: Microbiologia do Ar
Levivirus/isolamento & purificação
Orthomyxoviridae/isolamento & purificação
Virologia/métodos
[Mh] Termos MeSH secundário: Aerossóis
Resinas de Troca de Ânions
Seres Humanos
Levivirus/genética
Masculino
RNA Viral
Manejo de Espécimes/métodos
Virologia/instrumentação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aerosols); 0 (Anion Exchange Resins); 0 (RNA, Viral)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


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[PMID]:29346441
[Au] Autor:Gifford SC; Strachan BC; Xia H; Vörös E; Torabian K; Tomasino TA; Griffin GD; Lichtiger B; Aung FM; Shevkoplyas SS
[Ad] Endereço:Halcyon Biomedical Incorporated, Friendswood, Texas, United States of America.
[Ti] Título:A portable system for processing donated whole blood into high quality components without centrifugation.
[So] Source:PLoS One;13(1):e0190827, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The use of centrifugation-based approaches for processing donated blood into components is routine in the industrialized world, as disparate storage conditions require the rapid separation of 'whole blood' into distinct red blood cell (RBC), platelet, and plasma products. However, the logistical complications and potential cellular damage associated with centrifugation/apheresis manufacturing of blood products are well documented. The objective of this study was to evaluate a proof-of-concept system for whole blood processing, which does not employ electromechanical parts, is easily portable, and can be operated immediately after donation with minimal human labor. METHODS AND FINDINGS: In a split-unit study (n = 6), full (~500mL) units of freshly-donated whole blood were divided, with one half processed by conventional centrifugation techniques and the other with the new blood separation system. Each of these processes took 2-3 hours to complete and were performed in parallel. Blood products generated by the two approaches were compared using an extensive panel of cellular and plasma quality metrics. Comparison of nearly all RBC parameters showed no significant differences between the two approaches, although the portable system generated RBC units with a slight but statistically significant improvement in 2,3-diphosphoglyceric acid concentration (p < 0.05). More notably, several markers of platelet damage were significantly and meaningfully higher in products generated with conventional centrifugation: the increase in platelet activation (assessed via P-selectin expression in platelets before and after blood processing) was nearly 4-fold higher for platelet units produced via centrifugation, and the release of pro-inflammatory mediators (soluble CD40-ligand, thromboxane B2) was significantly higher for centrifuged platelets as well (p < 0.01). CONCLUSION: This study demonstrated that a simple, passive system for separating donated blood into components may be a viable alternative to centrifugation-particularly for applications in remote or resource-limited settings, or for patients requiring highly functional platelet product.
[Mh] Termos MeSH primário: Doadores de Sangue
Sangue
Manejo de Espécimes
[Mh] Termos MeSH secundário: Centrifugação
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180119
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190827


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[PMID]:25815882
[Au] Autor:Chen H; Ma S; Wu X; Yang W; Zhao T
[Ti] Título:Diagnose human colonic tissues by terahertz near-field imaging.
[So] Source:J Biomed Opt;20(3):036017, 2015 Mar.
[Is] ISSN:1560-2281
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Based on a terahertz (THz) pipe-based near-field imaging system, we demonstrate the capability of THz imaging to diagnose freshly surgically excised human colonic tissues. Through THz near-field scanning the absorbance of the colonic tissues, the acquired images can clearly distinguish cancerous tissues from healthy tissues fast and automatically without pathological hematoxylin and eosin stain diagnosis. A statistical study on 58 specimens (20 healthy tissues and 38 tissues with tumor) from 31 patients (mean age: 59 years; range: 46 to 79 years) shows that the corresponding diagnostic sensitivity and specificity on colonic tissues are both 100%. Due to its capability to perform quantitative analysis, our study indicates the potential of the THz pipe-based near-field imaging for future automation on human tumor pathological examinations.
[Mh] Termos MeSH primário: Colo/diagnóstico por imagem
Neoplasias do Colo/diagnóstico por imagem
Imagem Terahertz/métodos
[Mh] Termos MeSH secundário: Idoso
Diagnóstico Diferencial
Seres Humanos
Meia-Idade
Sensibilidade e Especificidade
Manejo de Espécimes/métodos
Coloração e Rotulagem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150328
[St] Status:MEDLINE
[do] DOI:10.1117/1.JBO.20.3.036017


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[PMID]:29406925
[Au] Autor:Canto MI; Montgomery E
[Ad] Endereço:Department of Medicine, Division of Gastroenterology and Hepatology, Baltimore, Maryland, USA.
[Ti] Título:Wide-area transepithelial sampling with 3-dimensional cytology: Does it detect more dysplasia or yield more hype?
[So] Source:Gastrointest Endosc;87(2):356-359, 2018 02.
[Is] ISSN:1097-6779
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Citodiagnóstico
Manejo de Espécimes
[Mh] Termos MeSH secundário: Hiperplasia
[Pt] Tipo de publicação:EDITORIAL; COMMENT
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180207
[St] Status:MEDLINE


  7 / 24943 MEDLINE  
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[PMID]:29401883
[Au] Autor:Anastasiadi G; Leonard M; Paterson L; Macpherson WN
[Ti] Título:Fabrication and characterization of machined multi-core fiber tweezers for single cell manipulation.
[So] Source:Opt Express;26(3):3557-3567, 2018 Feb 05.
[Is] ISSN:1094-4087
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Optical tweezing is a non-invasive technique that can enable a variety of single cell experiments; however, it tends to be based on a high numerical aperture (NA) microscope objective to both deliver the tweezing laser light and image the sample. This introduces restrictions in system flexibility when both trapping and imaging. Here, we demonstrate a novel, high NA tweezing system based on micro-machined multicore optical fibers. Using the machined, multicore fiber tweezer, cells are optically manipulated under a variety of microscopes, without requiring a high NA objective lens. The maximum NA of the fiber-based tweezer demonstrated is 1.039. A stable trap with a maximum total power 30 mW has been characterized to exert a maximum optical force of 26.4 pN, on a trapped, 7 µm diameter yeast cell. Single cells are held 15-35 µm from the fiber end and can be manipulated in the x, y and z directions throughout the sample. In this way, single cells are controllably trapped under a Raman microscope to categorize the yeast cells as live or dead, demonstrating trapping by the machined multicore fiber-based tweezer decoupled from the imaging or excitation objective lens.
[Mh] Termos MeSH primário: Fibras Ópticas
Pinças Ópticas
Manejo de Espécimes/métodos
Leveduras/citologia
[Mh] Termos MeSH secundário: Imagem Tridimensional/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180207
[St] Status:MEDLINE
[do] DOI:10.1364/OE.26.003557


  8 / 24943 MEDLINE  
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[PMID]:29038002
[Au] Autor:Guichet E; Serrano L; Laurent C; Eymard-Duvernay S; Kuaban C; Vidal L; Delaporte E; Ngole EM; Ayouba A; Peeters M
[Ad] Endereço:UMI233-TransVIHMI/INSERM U1175, Institut de Recherche pour le Développement (IRD) and University of Montpellier, Montpellier, France.
[Ti] Título:Comparison of different nucleic acid preparation methods to improve specific HIV-1 RNA isolation for viral load testing on dried blood spots.
[So] Source:J Virol Methods;251:75-79, 2018 Jan.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In resource-limited countries (RLCs), WHO recommends HIV viral load (VL) on dried blood spots (DBS) for antiretroviral therapy (ART) monitoring of patients living in non-urban settings where plasma VL is not available. In order to reduce the impact of proviral DNA interference, leading to false positive results in samples with low plasma VL, we compared three different nucleic acid preparation methods with the NucliSens (Biomérieux) extraction, known for its high recovery of nucleic acids on DBS. Paired plasma-DBS samples (n=151) with predominantly low plasma VL (≤10,000 copies/ml; 74%) were used. At the threshold of 1,000 copies/ml on DBS, 51% and 10% were misclassified as false positives or false negatives, respectively with NucliSens, versus 41% and 20% with m2000sp (Abbott), described as more specific for RNA recovery. DNase treatments of nucleic acid extracts and free virus elution (FVE) protocol before nucleic acid extraction, reduced the proportion of false positives to 0% and 19%, but increased the proportion of false negatives to 40% and 73%. More efforts are thus still needed to improve performance of VL assays on DBS to monitor patients on ART in RLCs and allow timely switch to more costly second or third line ART regimes.
[Mh] Termos MeSH primário: Infecções por HIV/virologia
HIV-1/isolamento & purificação
Plasma/virologia
RNA Viral/análise
RNA Viral/isolamento & purificação
Manejo de Espécimes/métodos
Carga Viral/métodos
[Mh] Termos MeSH secundário: Reações Falso-Negativas
Reações Falso-Positivas
Seres Humanos
[Pt] Tipo de publicação:COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171018
[St] Status:MEDLINE


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[PMID]:28986291
[Au] Autor:Meli ML; Berger A; Willi B; Spiri AM; Riond B; Hofmann-Lehmann R
[Ad] Endereço:Clinical Laboratory, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland; Center for Clinical Studies, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland. Electronic address: mmeli@vetclinics.uzh.ch.
[Ti] Título:Molecular detection of feline calicivirus in clinical samples: A study comparing its detection by RT-qPCR directly from swabs and after virus isolation.
[So] Source:J Virol Methods;251:54-60, 2018 Jan.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Feline caliciviruses (FCVs) are non-enveloped RNA viruses that exhibit high genetic variation. Two reverse transcription quantitative polymerase chain reaction (RT-qPCR) FCV assays (S1 and S2) were evaluated using samples from 300 field cats. The direct detection of FCV in swabs and after propagation in cell culture, as well as the influence of storage conditions, was assessed. FCV-RNA detectability on dry swabs was similar after storage at either 4°C or -20°C, but viral burdens were maintained for a longer time period when viral transport medium was used. A total of 97 (32%) samples was considered FCV PCR-positive. Of these, 81% and 77% tested positive directly from swabs using S1 and S2, respectively; 84% and 81% tested positive after enrichment in cell culture, respectively. Combined detection by RT-PCR directly from swabs and after VI was most sensitive (up to 96%). Neither of the methods alone were able to detect all FCV-positive samples. In conclusion, clinical samples should be collected in viral transport medium, stored at ≤4°C and processed as soon as possible. The combination of cell culture with RT-qPCR or detection directly from swabs using a combination of different RT-qPCR assays is recommended to reach a high sensitivity of FCV detection.
[Mh] Termos MeSH primário: Infecções por Caliciviridae/veterinária
Calicivirus Felino/isolamento & purificação
Doenças do Gato/diagnóstico
Doenças do Gato/virologia
Técnicas de Diagnóstico Molecular/métodos
Reação em Cadeia da Polimerase em Tempo Real/métodos
Manejo de Espécimes/métodos
[Mh] Termos MeSH secundário: Animais
Infecções por Caliciviridae/diagnóstico
Infecções por Caliciviridae/virologia
Gatos
Sensibilidade e Especificidade
Cultura de Vírus
[Pt] Tipo de publicação:COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171008
[St] Status:MEDLINE


  10 / 24943 MEDLINE  
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[PMID]:28746979
[Au] Autor:Liu Z; Pan A; Wu B; Zhou L; He H; Meng Q; Chen S; Pang Y; Wang X
[Ad] Endereço:The Institute of TB Control, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China.
[Ti] Título:Feasibility of a new model for early detection of patients with multidrug-resistant tuberculosis in a developed setting of eastern China.
[So] Source:Trop Med Int Health;22(10):1328-1333, 2017 10.
[Is] ISSN:1365-3156
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: The poor detection rate of multidrug-resistant tuberculosis (MDR-TB) highlights the urgent need to explore new case finding model to improve the detection of MDR-TB in China. The aim of this study was to evaluate the feasibility of a new model that combines molecular diagnostics and sputum transportation for early detection of patients with MDR-TB in Zhejiang. METHODS: From May 2014 to January 2015, TB suspects were continuously enrolled at six county-level designated TB hospitals in Zhejiang. Each patient gave three sputum samples, which were submitted to laboratory for smear microscopy, solid culture and GeneXpert. The specimens from rifampin (RIF)-resistant cases detected by GeneXpert, and positive cultures were transported from county-level to prefecture-level laboratories for line probe analysis (LPA) and drug susceptibility testing (DST). The performance and interval of MDR-TB detection of the new model were compared with those of conventional model. RESULTS: A total of 3151 sputum specimens were collected from TB suspects. The sensitivity of GeneXpert for detecting culture-positive cases was 92.7% (405/437), and its specificity was 91.3% (2428/2659). Of 16 RIF-resistant cases detected by DST, GeneXpert could correctly identify 15 cases, yielding a sensitivity of 93.8% (15/16). The specificity of GeneXpert for detecting RIF susceptibility was 100.0% (383/383). The average interval to diagnosis of the conventional DST model was 56.5 days, ranging from 43 to 71 days, which was significantly longer than that of GeneXpert plus LPA (22.2 days, P < 0.01). CONCLUSION: Our data demonstrate that the combination of improved molecular TB tests and sputum transportation could significantly shorten the time required for detection of MDR-TB, which will bring benefits for preventing an epidemic of MDR-TB in this high-prevalence setting.
[Mh] Termos MeSH primário: Mycobacterium tuberculosis/genética
Manejo de Espécimes/normas
Tuberculose Resistente a Múltiplos Medicamentos/diagnóstico
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana/métodos
China
Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos
Farmacorresistência Bacteriana Múltipla/genética
Diagnóstico Precoce
Estudos de Viabilidade
Técnicas de Genotipagem
Seres Humanos
Modelos Biológicos
Técnicas de Diagnóstico Molecular
Reação em Cadeia da Polimerase Multiplex
Mycobacterium tuberculosis/efeitos dos fármacos
Mycobacterium tuberculosis/isolamento & purificação
Sensibilidade e Especificidade
Manejo de Espécimes/métodos
Escarro/microbiologia
Tuberculose Resistente a Múltiplos Medicamentos/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1111/tmi.12934



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