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  1 / 206 MEDLINE  
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[PMID]:28463571
[Au] Autor:Penaud-Budloo M; Lecomte E; Guy-Duché A; Saleun S; Roulet A; Lopez-Roques C; Tournaire B; Cogné B; Léger A; Blouin V; Lindenbaum P; Moullier P; Ayuso E
[Ad] Endereço:1 INSERM UMR1089, University of Nantes, Centre Hospitalier Universitaire, Nantes, France.
[Ti] Título:Accurate Identification and Quantification of DNA Species by Next-Generation Sequencing in Adeno-Associated Viral Vectors Produced in Insect Cells.
[So] Source:Hum Gene Ther Methods;28(3):148-162, 2017 06.
[Is] ISSN:1946-6544
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Recombinant adeno-associated viral (rAAV) vectors have proven excellent tools for the treatment of many genetic diseases and other complex diseases. However, the illegitimate encapsidation of DNA contaminants within viral particles constitutes a major safety concern for rAAV-based therapies. Moreover, the development of rAAV vectors for early-phase clinical trials has revealed the limited accuracy of the analytical tools used to characterize these new and complex drugs. Although most published data concerning residual DNA in rAAV preparations have been generated by quantitative PCR, we have developed a novel single-strand virus sequencing (SSV-Seq) method for quantification of DNA contaminants in AAV vectors produced in mammalian cells by next-generation sequencing (NGS). Here, we describe the adaptation of SSV-Seq for the accurate identification and quantification of DNA species in rAAV stocks produced in insect cells. We found that baculoviral DNA was the most abundant contaminant, representing less than 2.1% of NGS reads regardless of serotype (2, 8, or rh10). Sf9 producer cell DNA was detected at low frequency (≤0.03%) in rAAV lots. Advanced computational analyses revealed that (1) baculoviral sequences close to the inverted terminal repeats preferentially underwent illegitimate encapsidation, and (2) single-nucleotide variants were absent from the rAAV genome. The high-throughput sequencing protocol described here enables effective DNA quality control of rAAV vectors produced in insect cells, and is adapted to conform with regulatory agency safety requirements.
[Mh] Termos MeSH primário: Dependovirus/genética
Sequenciamento de Nucleotídeos em Larga Escala/métodos
Análise de Sequência de DNA/métodos
[Mh] Termos MeSH secundário: Animais
Baculoviridae/genética
Contaminação por DNA
Células HEK293
Sequenciamento de Nucleotídeos em Larga Escala/normas
Seres Humanos
Análise de Sequência de DNA/normas
Células Sf9
Spodoptera
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180310
[Lr] Data última revisão:
180310
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1089/hgtb.2016.185


  2 / 206 MEDLINE  
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[PMID]:28575840
[Au] Autor:Templeton JEL; Taylor D; Handt O; Linacre A
[Ad] Endereço:School of Biological Sciences, Flinders University, Bedford Park, Adelaide, South Australia, 5042, Australia. Electronic address: jennifer.templeton@flinders.edu.au.
[Ti] Título:Typing DNA profiles from previously enhanced fingerprints using direct PCR.
[So] Source:Forensic Sci Int Genet;29:276-282, 2017 Jul.
[Is] ISSN:1878-0326
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Fingermarks are a source of human identification both through the ridge patterns and DNA profiling. Typing nuclear STR DNA markers from previously enhanced fingermarks provides an alternative method of utilising the limited fingermark deposit that can be left behind during a criminal act. Dusting with fingerprint powders is a standard method used in classical fingermark enhancement and can affect DNA data. The ability to generate informative DNA profiles from powdered fingerprints using direct PCR swabs was investigated. Direct PCR was used as the opportunity to generate usable DNA profiles after performing any of the standard DNA extraction processes is minimal. Omitting the extraction step will, for many samples, be the key to success if there is limited sample DNA. DNA profiles were generated by direct PCR from 160 fingermarks after treatment with one of the following dactyloscopic fingerprint powders: white hadonite; silver aluminium; HiFi Volcano silk black; or black magnetic fingerprint powder. This was achieved by a combination of an optimised double-swabbing technique and swab media, omission of the extraction step to minimise loss of critical low-template DNA, and additional AmpliTaq Gold DNA polymerase to boost the PCR. Ninety eight out of 160 samples (61%) were considered 'up-loadable' to the Australian National Criminal Investigation DNA Database (NCIDD). The method described required a minimum of working steps, equipment and reagents, and was completed within 4h. Direct PCR allows the generation of DNA profiles from enhanced prints without the need to increase PCR cycle numbers beyond manufacturer's recommendations. Particular emphasis was placed on preventing contamination by applying strict protocols and avoiding the use of previously used fingerprint brushes. Based on this extensive survey, the data provided indicate minimal effects of any of these four powders on the chance of obtaining DNA profiles from enhanced fingermarks.
[Mh] Termos MeSH primário: Impressões Digitais de DNA
Dermatoglifia
Reação em Cadeia da Polimerase/métodos
[Mh] Termos MeSH secundário: Contaminação por DNA
DNA Polimerase Dirigida por DNA
Seres Humanos
Pós
Manejo de Espécimes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Powders); EC 2.7.7.7 (DNA-Directed DNA Polymerase)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170707
[Lr] Data última revisão:
170707
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170603
[St] Status:MEDLINE


  3 / 206 MEDLINE  
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[PMID]:28356154
[Au] Autor:Ballenghien M; Faivre N; Galtier N
[Ad] Endereço:UMR5554 - Institute of Evolutionary Sciences, University Montpellier, CNRS, IRD, EPHE, Place Eugène Bataillon, CC64, 34095, Montpellier, France.
[Ti] Título:Patterns of cross-contamination in a multispecies population genomic project: detection, quantification, impact, and solutions.
[So] Source:BMC Biol;15(1):25, 2017 Mar 29.
[Is] ISSN:1741-7007
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Contamination is a well-known but often neglected problem in molecular biology. Here, we investigated the prevalence of cross-contamination among 446 samples from 116 distinct species of animals, which were processed in the same laboratory and subjected to subcontracted transcriptome sequencing. RESULTS: Using cytochrome oxidase 1 as a barcode, we identified a minimum of 782 events of between-species contamination, with approximately 80% of our samples being affected. An analysis of laboratory metadata revealed a strong effect of the sequencing center: nearly all the detected events of between-species contamination involved species that were sent the same day to the same company. We introduce new methods to address the amount of within-species, between-individual contamination, and to correct for this problem when calling genotypes from base read counts. CONCLUSIONS: We report evidence for pervasive within-species contamination in this data set, and show that classical population genomic statistics, such as synonymous diversity, the ratio of non-synonymous to synonymous diversity, inbreeding coefficient F , and Tajima's D, are sensitive to this problem to various extents. Control analyses suggest that our published results are probably robust to the problem of contamination. Recommendations on how to prevent or avoid contamination in large-scale population genomics/molecular ecology are provided based on this analysis.
[Mh] Termos MeSH primário: Contaminação por DNA
Genética Populacional
Genômica
[Mh] Termos MeSH secundário: Bases de Dados Genéticas
Complexo IV da Cadeia de Transporte de Elétrons/genética
Metagenômica
Polimorfismo de Nucleotídeo Único/genética
Probabilidade
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 1.9.3.1 (Electron Transport Complex IV)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170331
[St] Status:MEDLINE
[do] DOI:10.1186/s12915-017-0366-6


  4 / 206 MEDLINE  
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[PMID]:28322595
[Au] Autor:Schnödt M; Büning H
[Ad] Endereço:1 Center for Molecular Medicine Cologne, University of Cologne , Cologne, Germany .
[Ti] Título:Improving the Quality of Adeno-Associated Viral Vector Preparations: The Challenge of Product-Related Impurities.
[So] Source:Hum Gene Ther Methods;28(3):101-108, 2017 Jun.
[Is] ISSN:1946-6544
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Adeno-associated viral (AAV) vectors have emerged as one of the most popular gene transfer systems in both research and clinical gene therapy. As AAV vectors are derived from a stealth, nonpathogenic virus and lack active integrase activity, these vectors are frequently applied for in vivo gene therapy of liver, muscle, and other postmitotic tissues. Although long-term transgene expression from AAV vector episomes is reported from these tissues, the episomal nature of AAV-once regarded as disadvantage-has become an attractive feature for gene-editing approaches targeting proliferating cells. In response to the high demand, AAV vector production is receiving special attention. Besides particle yields and biological activity, the most important concern is improving vector purity. The most difficult task in this regard is removal of defective particles, that is, capsids that are either empty or contain DNA other than the full-length vector genomes. Herein, we characterize and discuss these so-called product-related impurities, methods for their detection, as well as strategies to avoid or reduce their formation.
[Mh] Termos MeSH primário: Dependovirus/genética
Vetores Genéticos/normas
[Mh] Termos MeSH secundário: Animais
Contaminação por DNA
Terapia Genética/métodos
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1089/hgtb.2016.188


  5 / 206 MEDLINE  
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[PMID]:28225826
[Au] Autor:Jean A; Tardy F; Allatif O; Grosjean I; Blanquier B; Gerlier D
[Ad] Endereço:Univ Lyon, SFR BioSciences, ENS de Lyon, Inserm US8, CNRS UMS344, UCBL, Lyon, France.
[Ti] Título:Assessing mycoplasma contamination of cell cultures by qPCR using a set of universal primer pairs targeting a 1.5 kb fragment of 16S rRNA genes.
[So] Source:PLoS One;12(2):e0172358, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mycoplasmas (a generic name for Mollicutes) are a predominant bacterial contaminant of cell culture and cell derived products including viruses. This prokaryote class is characterized by very small size and lack of a cell wall. Consequently, mycoplasmas escape ultrafiltration and visualization under routine microscopic examination, hence the ease with which cells in culture can be contaminated, with routinely more than 10% of cell lines being contaminated. Mycoplasma are a formidable threat both in fundamental research by perverting a whole range of cell properties and functions and in the pharmacological use of cells and cell derived products. Although many methods have been developed, there is still a need for a sensitive, universal assay. Here is reported the development and validation of a quantitative polymerase chain reaction (qPCR) based on the amplification of a 1.5 kb fragment covering the 16S rDNA of the Mollicute class by real-time PCR using universal U1 and U8 degenerate primers. The method includes the addition of a DNA loading probe to each sample to monitor DNA extraction and the absence of PCR inhibitors in the extracted DNA, a positive mycoplasma 16S rDNA traceable reference sample to exclude any accidental contamination of an unknown sample with this reference DNA, an analysis procedure based on the examination of the melting curve and the size of the PCR amplicon, followed by quantification of the number of 16S rDNA copies (with a lower limit of 19 copies) when relevant, and, if useful, the identification of the contaminating prokaryote by sequencing. The method was validated on a collection of mycoplasma strains and by testing over 100 samples of unknown contamination status including stocks of viruses requiring biosafety level 2, 3 or 4 containments. When compared to four established methods, the m16S_qPCR technique exhibits the highest sensitivity in detecting mycoplasma contamination.
[Mh] Termos MeSH primário: Contaminação por DNA
Mycoplasma/isolamento & purificação
RNA Ribossômico 16S/genética
[Mh] Termos MeSH secundário: Animais
Técnicas de Cultura de Células
Linhagem Celular
Cricetinae
Primers do DNA/genética
DNA Bacteriano/genética
Haplorrinos
Seres Humanos
Iguanas
Camundongos
Mycoplasma/genética
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170223
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0172358


  6 / 206 MEDLINE  
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[PMID]:28225823
[Au] Autor:Ao W; Clifford A; Corpuz M; Jenison R
[Ad] Endereço:Great Basin Corporation, Salt Lake City, Utah, United States of America.
[Ti] Título:A novel approach to eliminate detection of contaminating Staphylococcal species introduced during clinical testing.
[So] Source:PLoS One;12(2):e0171915, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We describe here a strategy that can distinguish between Staphylococcus species truly present in a clinical sample from contaminating Staphylococcus species introduced during the testing process. Contaminating Staphylococcus species are present at low levels in PCR reagents and colonize lab personnel. To eliminate detection of contaminants, we describe an approach that utilizes addition of sufficient quantities of either non-target Staphylococcal cells (Staphylococcus succinus or Staphylococcus muscae) or synthetic oligonucleotide templates to helicase dependent isothermal amplification reactions to consume Staphylococcus-specific tuf and mecA gene primers such that contaminating Staphylococcus amplification is suppressed to below assay limits of detection. The suppressor template DNA is designed with perfect homology to the primers used in the assay but an internal sequence that is unrelated to the Staphylococcal species targeted for detection. Input amount of the suppressor is determined by a mathematical model described herein and is demonstrated to completely suppress contaminating levels of Staphylococcus while not negatively impacting the appropriate clinical assay limit of detection. We have applied this approach to improve the specificity of detection of Staphylococcus species present in positive blood cultures using a chip-based array that produces results visible to the unaided eye.
[Mh] Termos MeSH primário: Contaminação por DNA
DNA Bacteriano
Técnicas de Amplificação de Ácido Nucleico/métodos
Infecções Estafilocócicas/diagnóstico
Staphylococcus/isolamento & purificação
[Mh] Termos MeSH secundário: Seres Humanos
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170223
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0171915


  7 / 206 MEDLINE  
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[PMID]:28109256
[Au] Autor:Castelino M; Eyre S; Moat J; Fox G; Martin P; Ho P; Upton M; Barton A
[Ad] Endereço:NIHR Manchester Musculoskeletal Biomedical research Unit, Central Manchester Foundation Trust, Manchester Academic Health Sciences Centre, Manchester, England.
[Ti] Título:Optimisation of methods for bacterial skin microbiome investigation: primer selection and comparison of the 454 versus MiSeq platform.
[So] Source:BMC Microbiol;17(1):23, 2017 Jan 21.
[Is] ISSN:1471-2180
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The composition of the skin microbiome is predicted to play a role in the development of conditions such as atopic eczema and psoriasis. 16S rRNA gene sequencing allows the investigation of bacterial microbiota. A significant challenge in this field is development of cost effective high throughput methodologies for the robust interrogation of the skin microbiota, where biomass is low. Here we describe validation of methodologies for 16S rRNA (ribosomal ribonucleic acid) gene sequencing from the skin microbiome, using the Illumina MiSeq platform, the selection of primer to amplify regions for sequencing and we compare results with the current standard protocols.. METHODS: DNA was obtained from two low density mock communities of 11 diverse bacterial strains (with and without human DNA supplementation) and from swabs taken from the skin of healthy volunteers. This was amplified using primer pairs covering hypervariable regions of the 16S rRNA gene: primers 63F and 519R (V1-V3); and 347F and 803R (V3-V4). The resultant libraries were indexed for the MiSeq and Roche454 and sequenced. Both data sets were denoised, cleaned of chimeras and analysed using QIIME. RESULTS: There was no significant difference in the diversity indices at the phylum and the genus level observed between the platforms. The capture of diversity using the low density mock community samples demonstrated that the primer pair spanning the V3-V4 hypervariable region had better capture when compared to the primer pair for the V1-V3 region and was robust to spiking with human DNA. The pilot data generated using the V3-V4 region from the skin of healthy volunteers was consistent with these results, even at the genus level (Staphylococcus, Propionibacterium, Corynebacterium, Paracoccus, Micrococcus, Enhydrobacter and Deinococcus identified at similar abundances on both platforms). CONCLUSIONS: The results suggest that the bacterial community diversity captured using the V3-V4 16S rRNA hypervariable region from sequencing using the MiSeq platform is comparable to the Roche454 GS Junior platform. These findings provide evidence that the optimised method can be used in human clinical samples of low bacterial biomass such as the investigation of the skin microbiota.
[Mh] Termos MeSH primário: Bactérias/genética
Bactérias/isolamento & purificação
Técnicas de Tipagem Bacteriana/métodos
Sequenciamento de Nucleotídeos em Larga Escala/métodos
Microbiota/genética
Pele/microbiologia
[Mh] Termos MeSH secundário: Adulto
Bactérias/classificação
Sequência de Bases
Biomassa
Biologia Computacional/métodos
Contaminação por DNA
Primers do DNA/genética
DNA Bacteriano/genética
DNA Bacteriano/isolamento & purificação
Genes Bacterianos
Variação Genética
Sequenciamento de Nucleotídeos em Larga Escala/instrumentação
Seres Humanos/microbiologia
Meia-Idade
Filogenia
Reação em Cadeia da Polimerase/métodos
RNA Ribossômico 16S/genética
Análise de Sequência de DNA/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170831
[Lr] Data última revisão:
170831
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170123
[St] Status:MEDLINE
[do] DOI:10.1186/s12866-017-0927-4


  8 / 206 MEDLINE  
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[PMID]:28052076
[Au] Autor:Schwarzer M; Srutkova D; Hermanova P; Leulier F; Kozakova H; Schabussova I
[Ad] Endereço:Laboratory of Gnotobiology, Institute of Microbiology of the Czech Academy of Sciences, v. v. i., Novy Hradek, Czech Republic.
[Ti] Título:Diet Matters: Endotoxin in the Diet Impacts the Level of Allergic Sensitization in Germ-Free Mice.
[So] Source:PLoS One;12(1):e0167786, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Germ-free animals have been used to define the vital role of commensal bacteria on the maturation of the host immune system. However, the role of bacterial residues in diet in this setting is poorly understood. Here we investigated the effect of bacterial contamination in sterile diet on the level of allergic sensitization in germ-free mice. Sterile grain-based diets ST1 and R03 were tested for the level of bacterial contamination. ST1 contained higher amount of bacterial DNA, approximately ten times more endotoxin, and induced higher, TLR4-dependent, cytokine production in dendritic cells compared to R03. In a germ-free mouse model of sensitization to the major birch pollen allergen Bet v 1, feeding on ST1 for at least two generations was associated with decreased production of allergen-specific IgE and IgG1 antibodies in sera in comparison to R03. Furthermore, reduced levels of allergen-specific and ConA-induced cytokines IL-4, IL-5 and IL-13 accompanied by increased levels of IFN-γ were detected in splenocytes cultures of these mice. Our results show that contamination of experimental diet with bacterial residues, such as endotoxin, significantly affects the development of allergic sensitization in germ-free mice. Therefore, careful selection of sterile food is critical for the outcomes of germ-free or gnotobiotic experimental models of immune-deviated diseases.
[Mh] Termos MeSH primário: Dieta
Endotoxinas/toxicidade
Hipersensibilidade/imunologia
Imunização
[Mh] Termos MeSH secundário: Animais
Antígenos de Plantas/imunologia
Cruzamento
Diferenciação Celular/efeitos dos fármacos
Células Cultivadas
Citocinas/biossíntese
Contaminação por DNA
DNA Bacteriano/análise
Células Dendríticas/efeitos dos fármacos
Sistema Digestório/imunologia
Epitopos/imunologia
Vida Livre de Germes
Células HEK293
Seres Humanos
Hipersensibilidade/patologia
Imunoglobulina A/imunologia
Imunoglobulina G/imunologia
Ligantes
Camundongos Endogâmicos BALB C
Mitógenos/farmacologia
Baço/patologia
Receptor 4 Toll-Like/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Plant); 0 (Cytokines); 0 (DNA, Bacterial); 0 (Endotoxins); 0 (Epitopes); 0 (Immunoglobulin A); 0 (Immunoglobulin G); 0 (Ligands); 0 (Mitogens); 0 (Toll-Like Receptor 4); 126161-14-6 (Bet v 1 allergen, Betula)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170105
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0167786


  9 / 206 MEDLINE  
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[PMID]:28003257
[Au] Autor:Schröder J; Corbin V; Papenfuss AT
[Ad] Endereço:Bioinformatics Division, The Walter & Eliza Hall Institute, Parkville 3052, Australia.
[Ti] Título:HYSYS: have you swapped your samples?
[So] Source:Bioinformatics;33(4):596-598, 2017 Feb 15.
[Is] ISSN:1367-4811
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Motivation: The application of a genomics assay to samples from a cohort is a frequently applied experimental design in cancer genomics studies. The collection and analysis of cancer sequencing data in the clinical setting is an elaborate process that may involve consenting patients, obtaining possibly-multiple DNA samples, sequencing and analysis. Many of these steps are manual. At any stage mistakes can occur that cause a DNA sample to be labelled incorrectly. However, there is a paucity of methods in the literature to identify such swaps specifically in cancer studies. Results: Here, we introduce a simple method, HYSYS, to estimate the relatedness of samples and test for sample swaps and contamination. The test uses the concordance of homozygous SNPs between samples. The method is motivated by the observation that homozygous germline population variants rarely change in the disease and are not affected by loss of heterozygosity. Our tools include visualization and a testing framework to flag possible sample swaps. We demonstrate the utility of this approach on a small cohort. Availability and Implementation: http://github.com/PapenfussLab/HaveYouSwappedYourSamples. Contact: papenfuss@wehi.edu.au. Supplementary information: Supplementary data are available at Bioinformatics online.
[Mh] Termos MeSH primário: Genômica/métodos
Neoplasias/genética
Controle de Qualidade
Software
[Mh] Termos MeSH secundário: Contaminação por DNA
Seres Humanos
Polimorfismo de Nucleotídeo Único
Análise de Sequência de DNA/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161223
[St] Status:MEDLINE
[do] DOI:10.1093/bioinformatics/btw685


  10 / 206 MEDLINE  
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[PMID]:27979779
[Au] Autor:Wichard JD
[Ad] Endereço:Bayer Pharma AG, Genetic Toxicology, Müllerstr. 178, D-13353, Berlin, Germany. Electronic address: joerg.wichard@bayer.com.
[Ti] Título:In silico prediction of genotoxicity.
[So] Source:Food Chem Toxicol;106(Pt B):595-599, 2017 Aug.
[Is] ISSN:1873-6351
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The in silico prediction of genotoxicity has made considerable progress during the last years. The main driver for the pharmaceutical industry is the ICH M7 guideline about the assessment of DNA reactive impurities. An important component of this guideline is the use of in silico models as an alternative approach to experimental testing. The in silico prediction of genotoxicity provides an established and accepted method that defines the first step in the assessment of DNA reactive impurities. This was made possible by the growing amount of reliable Ames screening data, the attempts to understand the activity pathways and the subsequent development of computer-based prediction systems. This paper gives an overview of how the in silico prediction of genotoxicity is performed under the ICH M7 guideline.
[Mh] Termos MeSH primário: Testes de Mutagenicidade/métodos
Mutagênicos/toxicidade
[Mh] Termos MeSH secundário: Animais
Simulação por Computador
DNA/análise
DNA/genética
Contaminação por DNA
Dano ao DNA/efeitos dos fármacos
Seres Humanos
Testes de Mutagenicidade/normas
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Mutagens); 9007-49-2 (DNA)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170831
[Lr] Data última revisão:
170831
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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde