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[PMID]:29348575
[Au] Autor:Neef J; Urban NT; Ohn TL; Frank T; Jean P; Hell SW; Willig KI; Moser T
[Ad] Endereço:Institute for Auditory Neuroscience and InnerEarLab, University Medical Center Göttingen, 37099, Göttingen, Germany.
[Ti] Título:Quantitative optical nanophysiology of Ca signaling at inner hair cell active zones.
[So] Source:Nat Commun;9(1):290, 2018 01 18.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Ca influx triggers the release of synaptic vesicles at the presynaptic active zone (AZ). A quantitative characterization of presynaptic Ca signaling is critical for understanding synaptic transmission. However, this has remained challenging to establish at the required resolution. Here, we employ confocal and stimulated emission depletion (STED) microscopy to quantify the number (20-330) and arrangement (mostly linear 70 nm × 100-600 nm clusters) of Ca channels at AZs of mouse cochlear inner hair cells (IHCs). Establishing STED Ca imaging, we analyze presynaptic Ca signals at the nanometer scale and find confined elongated Ca domains at normal IHC AZs, whereas Ca domains are spatially spread out at the AZs of bassoon-deficient IHCs. Performing 2D-STED fluorescence lifetime analysis, we arrive at estimates of the Ca concentrations at stimulated IHC AZs of on average 25 µM. We propose that IHCs form bassoon-dependent presynaptic Ca -channel clusters of similar density but scalable length, thereby varying the number of Ca channels amongst individual AZs.
[Mh] Termos MeSH primário: Sinalização do Cálcio/fisiologia
Células Ciliadas Auditivas Internas/fisiologia
Microscopia/métodos
Nanotecnologia/métodos
[Mh] Termos MeSH secundário: Algoritmos
Animais
Cálcio/metabolismo
Canais de Cálcio Tipo L/fisiologia
Células Ciliadas Auditivas Internas/metabolismo
Camundongos Endogâmicos C57BL
Camundongos Knockout
Microscopia Confocal
Modelos Neurológicos
Proteínas do Tecido Nervoso/genética
Proteínas do Tecido Nervoso/fisiologia
Sinapses/metabolismo
Sinapses/fisiologia
Transmissão Sináptica/genética
Transmissão Sináptica/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bsn protein, mouse); 0 (Calcium Channels, L-Type); 0 (Nerve Tissue Proteins); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180120
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02612-y


  2 / 28359 MEDLINE  
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[PMID]:29328893
[Au] Autor:Simon JF; Nanavati A
[Ad] Endereço:Department of Nephrology and Hypertension, Cleveland Clinic, Cleveland, OH, USA. simonj2@ccf.org.
[Ti] Título:Quality in urine microscopy: The eyes of the beholder.
[So] Source:Cleve Clin J Med;85(1):22-24, 2018 01.
[Is] ISSN:1939-2869
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Microscopia
Urinálise
[Mh] Termos MeSH secundário: Seres Humanos
[Pt] Tipo de publicação:EDITORIAL; COMMENT
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE
[do] DOI:10.3949/ccjm.85a.17085


  3 / 28359 MEDLINE  
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[PMID]:29183021
[Au] Autor:Chantziantoniou N; Mukherjee M; Donnelly AD; Pantanowitz L; Austin RM
[Ad] Endereço:Department of Pathology, Cytopathology Section, Sidra Medicine, Qatar Foundation, Doha, Qatar.
[Ti] Título:Digital Applications in Cytopathology: Problems, Rationalizations, and Alternative Approaches.
[So] Source:Acta Cytol;62(1):68-76, 2018.
[Is] ISSN:1938-2650
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: The aim of this work was to raise awareness of problems using digital applications for examining, teaching, and applying telecytology at King Abdulaziz Medical City (KAMC), Riyadh, Saudi Arabia; University of Nebraska Medical Center (UNMC), Omaha, NE, USA; and University of Pittsburgh Medical Center (UPMC), Pittsburgh, PA, USA. The objective was to rationalize problems and propose alternative digital approaches. STUDY DESIGN: We sought to identify solutions to improve the following: (a) interpretive examination scores at KAMC for complex cytological templates (i.e., high-grade squamous intraepithelial lesions [HSIL]) when using static digital images (SDI) of cells in regions of interest (ROI); (b) visualization of cells in 3D clusters when teaching at UNMC using 2D and 3D whole-slide imaging (WSI); and (c) visualization of cells through streaming telecytology at UPMC. RESULTS: Composite SDI (CSDI) improved test scores for complex interpretations (i.e., HSIL) by converging diagnostic criteria from multiple ROI. Multiplane focusing through z-stacked WSI facilitated the teaching of cytological entities characterized by 3D cell clusters and consultative telecytology through robotic cell analysis. CONCLUSIONS: Adequately visualized cytomorphology and multiplane focusing are essential for virtual cytopathology examinations, teaching, or consultative telecytology. Visualization of diagnostic criteria through 2D or 3D imaging is critical. Panoptiq panoramic WSI with integrated z-stacked video clips enables optimal applied telecytology.
[Mh] Termos MeSH primário: Instrução por Computador
Educação Médica/métodos
Interpretação de Imagem Assistida por Computador/métodos
Microscopia/métodos
Patologia/educação
Lesões Intraepiteliais Escamosas Cervicais/patologia
Telepatologia/métodos
Neoplasias do Colo do Útero/patologia
[Mh] Termos MeSH secundário: Feminino
Seres Humanos
Nebraska
Teste de Papanicolaou
Pennsylvania
Valor Preditivo dos Testes
Reprodutibilidade dos Testes
Arábia Saudita
Esfregaço Vaginal
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE
[do] DOI:10.1159/000484434


  4 / 28359 MEDLINE  
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[PMID]:29444047
[Au] Autor:Russell CT; Rees EJ; Kaminski CF
[Ti] Título:Homographically generated light sheets for the microscopy of large specimens.
[So] Source:Opt Lett;43(4):663-666, 2018 Feb 15.
[Is] ISSN:1539-4794
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We compare the performance of linear and nonlinear methods for aligning the excitation and detection planes throughout volumes of large specimens in digitally scanned light sheet microscopy. An effective nonlinear method involves the registration of four corner extrema of the imaging volume via a projective transform. We show that this improves the light collection efficiency of the commonly used three-point affine registration by an average of 42% over a typical specimen volume. Accurate illumination/detection registration methods are now pertinent to biological research in view of current trends towards imaging large or expanded samples, at depth, with diffraction limited resolution.
[Mh] Termos MeSH primário: Luz
Microscopia/métodos
[Mh] Termos MeSH secundário: Corantes Fluorescentes
Imagem Tridimensional
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fluorescent Dyes)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180215
[St] Status:MEDLINE
[do] DOI:10.1364/OL.43.000663


  5 / 28359 MEDLINE  
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[PMID]:29357378
[Au] Autor:Law YN; Jian H; Lo NWS; Ip M; Chan MMY; Kam KM; Wu X
[Ad] Endereço:Hong Kong Applied Science & Technology Research Institute Co., Ltd. (ASTRI), Hong Kong SAR, China.
[Ti] Título:Low cost automated whole smear microscopy screening system for detection of acid fast bacilli.
[So] Source:PLoS One;13(1):e0190988, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In countries with high tuberculosis (TB) burden, there is urgent need for rapid, large-scale screening to detect smear-positive patients. We developed a computer-aided whole smear screening system that focuses in real-time, captures images and provides diagnostic grading, for both bright-field and fluorescence microscopy for detection of acid-fast-bacilli (AFB) from respiratory specimens. OBJECTIVES: To evaluate the performance of dual-mode screening system in AFB diagnostic algorithms on concentrated smears with auramine O (AO) staining, as well as direct smears with AO and Ziehl-Neelsen (ZN) staining, using mycobacterial culture results as gold standard. METHODS: Adult patient sputum samples requesting for M. tuberculosis cultures were divided into three batches for staining: direct AO-stained, direct ZN-stained and concentrated smears AO-stained. All slides were graded by an experienced microscopist, in parallel with the automated whole smear screening system. Sensitivity and specificity of a TB diagnostic algorithm in using the screening system alone, and in combination with a microscopist, were evaluated. RESULTS: Of 488 direct AO-stained smears, 228 were culture positive. These yielded a sensitivity of 81.6% and specificity of 74.2%. Of 334 direct smears with ZN staining, 142 were culture positive, which gave a sensitivity of 70.4% and specificity of 76.6%. Of 505 concentrated smears with AO staining, 250 were culture positive, giving a sensitivity of 86.4% and specificity of 71.0%. To further improve performance, machine grading was confirmed by manual smear grading when the number of AFBs detected fell within an uncertainty range. These combined results gave significant improvement in specificity (AO-direct:85.4%; ZN-direct:85.4%; AO-concentrated:92.5%) and slight improvement in sensitivity while requiring only limited manual workload. CONCLUSION: Our system achieved high sensitivity without substantially compromising specificity when compared to culture results. Significant improvement in specificity was obtained when uncertain results were confirmed by manual smear grading. This approach had potential to substantially reduce workload of microscopists in high burden countries.
[Mh] Termos MeSH primário: Automação
Custos e Análise de Custo
Microscopia/métodos
Mycobacterium tuberculosis/isolamento & purificação
[Mh] Termos MeSH secundário: Seres Humanos
Microscopia/economia
Microscopia de Fluorescência
Escarro/microbiologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; VALIDATION STUDIES
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180123
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190988


  6 / 28359 MEDLINE  
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[PMID]:29216124
[Au] Autor:Trujillo C; Garcia-Sucerquia J
[Ti] Título:Phase-shifting digital holographic microscopy by using a multi-camera setup.
[So] Source:Opt Lett;42(23):4841-4844, 2017 Dec 01.
[Is] ISSN:1539-4794
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this Letter, the use of two-coupled Mach-Zehnder interferometers for four π/2-phase shifting interferometry is introduced. A multi-camera arrangement using no more than beam splitters and mirrors is utilized to obtain in a single shot the needed phase-shifted interferograms in the different output channels of the setup. The simplicity of the setup makes it ideal for high-speed interferometry applications. This proposal is validated in digital holographic microscopy to visualize a biological sample of epidermal onion cells.
[Mh] Termos MeSH primário: Holografia/instrumentação
Microscopia/instrumentação
[Mh] Termos MeSH secundário: Desenho de Equipamento
Interferometria/instrumentação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE
[do] DOI:10.1364/OL.42.004841


  7 / 28359 MEDLINE  
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[PMID]:29189794
[Ti] Título:A day in the life of a cell biologist.
[So] Source:Nature;551(7682):S182, 2017 11 30.
[Is] ISSN:1476-4687
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Biologia Celular
Pesquisadores
[Mh] Termos MeSH secundário: Pesquisa Interdisciplinar
Fusão de Membrana
Microscopia
Sinaptotagmina I/metabolismo
[Pt] Tipo de publicação:INTERVIEW
[Nm] Nome de substância:
0 (Synaptotagmin I)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE
[do] DOI:10.1038/d41586-017-07563-4


  8 / 28359 MEDLINE  
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[PMID]:28463472
[Au] Autor:Caromel AGM; Schmidt DN; Rayfield EJ
[Ad] Endereço:School of Earth Sciences, University of Bristol, Wills Memorial Building, Bristol, United Kingdom.
[Ti] Título:Ontogenetic constraints on foraminiferal test construction.
[So] Source:Evol Dev;19(3):157-168, 2017 05.
[Is] ISSN:1525-142X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Developmental processes represent one of the main constraints on the generation of adult form. Determining how constructional and energetic demands operate throughout growth is es-sential to understanding fundamental growth rules and trade-offs that define the framework within which new species originate. In organisms producing spiral shells, coiling patterns can inform on the constructional constraints acting throughout development that dictated the diversification of forms within a group. Here, we use Synchrotron radiation X-Ray tomographic microscopy (SRXTM) reconstructions of eight planktic foraminifera repre-sentative of the major morphotypic groups to determine disparity of coiling patterns by measuring Raupian parameters. The results show that foraminifera are a morphologically highly conservative group, exploiting a limited range of poten-tial coiling patterns. Very similar coiling patterns during early ontogeny, regardless of species, point toward strong constraints in early ontogeny and to common develop-mental processes acting across all morphogroups. Dispersion and lateral displacement of taxa in morphospace are limited to the adult stage. Accretion with low translation down the coiling axis in juveniles may maximize lateral growth and metabolic efficiency in light of costly calcification. Increased translation in the adult stages allows growth to accommo-date new chamber shapes, mediated by changes in aperture location and the site of accretion over ontogeny. These constructional constraints, and the accretion of a small number of discrete chambers, limit the potential for novel forms within the foraminifera compared to other groups of coiling organisms and may explain the repeated evolution of similar morphotypes throughout the evolutionary history of the group.
[Mh] Termos MeSH primário: Evolução Biológica
Foraminíferos/citologia
Foraminíferos/genética
[Mh] Termos MeSH secundário: Biometria
Foraminíferos/classificação
Microscopia/métodos
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1707
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1111/ede.12224


  9 / 28359 MEDLINE  
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[PMID]:29402037
[Au] Autor:Dong B; Booth MJ
[Ti] Título:Wavefront control in adaptive microscopy using Shack-Hartmann sensors with arbitrarily shaped pupils.
[So] Source:Opt Express;26(2):1655-1669, 2018 Jan 22.
[Is] ISSN:1094-4087
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In adaptive optical microscopy of thick biological tissue, strong scattering and aberrations can change the effective pupil shape by rendering some Shack-Hartmann spots unusable. The change of pupil shape leads to a change of wavefront reconstruction or control matrix that should be updated accordingly. Modified slope and modal wavefront control methods based on measurements of a Shack-Hartmann wavefront sensor are proposed to accommodate an arbitrarily shaped pupil. Furthermore, we present partial wavefront control methods that remove specific aberration modes like tip, tilt and defocus from the control loop. The proposed control methods were investigated and compared by simulation using experimentally obtained aberration data. The performance was then tested experimentally through closed-loop aberration corrections using an obscured pupil.
[Mh] Termos MeSH primário: Aberrometria/métodos
Iris/diagnóstico por imagem
Microscopia/métodos
Imagem Óptica/métodos
Pupila
[Mh] Termos MeSH secundário: Algoritmos
Animais
Encéfalo/diagnóstico por imagem
Caenorhabditis elegans
Iris/anatomia & histologia
Microscopia/instrumentação
Imagem Óptica/instrumentação
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180207
[St] Status:MEDLINE
[do] DOI:10.1364/OE.26.001655


  10 / 28359 MEDLINE  
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[PMID]:29402000
[Au] Autor:Rose M; Senkbeil T; von Gundlach AR; Stuhr S; Rumancev C; Dzhigaev D; Besedin I; Skopintsev P; Loetgering L; Viefhaus J; Rosenhahn A; Vartanyants IA
[Ti] Título:Quantitative ptychographic bio-imaging in the water window.
[So] Source:Opt Express;26(2):1237-1254, 2018 Jan 22.
[Is] ISSN:1094-4087
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Coherent X-ray ptychography is a tool for highly dose efficient lensless nano-imaging of biological samples. We have used partially coherent soft X-ray synchrotron radiation to obtain a quantitative image of a laterally extended, dried, and unstained fibroblast cell by ptychography. We used data with and without a beam stop that allowed us to measure coherent diffraction with a high-dynamic range of 1.7·10 . As a quantitative result, we obtained the refractive index values for two regions of the cell with respect to a reference area. Due to the photon energy in the water window we obtained an extremely high contrast of 53% at 71 nm half-period resolution. The dose applied in our experiment was 9.5·10 Gy and is well below the radiation damage threshold. The concept for dynamic range improvement for low dynamic range detectors with a beam stop opens the path for high resolution nano-imaging of a variety of samples including cryo-preserved, hydrated and unstained biological cells.
[Mh] Termos MeSH primário: Algoritmos
Fibroblastos
Microscopia/métodos
Intensificação de Imagem Radiográfica/métodos
Difração de Raios X
[Mh] Termos MeSH secundário: Fótons
Dose de Radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180207
[St] Status:MEDLINE
[do] DOI:10.1364/OE.26.001237



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