Base de dados : MEDLINE
Pesquisa : E01.370.350.515.666 [Categoria DeCS]
Referências encontradas : 544 [refinar]
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[PMID]:28650092
[Au] Autor:Hauf M; Richter F; Schneider T; Faidt T; Martins BM; Baumann T; Durkin P; Dobbek H; Jacobs K; Möglich A; Budisa N
[Ad] Endereço:Institut für Chemie, Technische Universität Berlin, Müller-Breslau-Strasse 10, 10623, Berlin, Germany.
[Ti] Título:Photoactivatable Mussel-Based Underwater Adhesive Proteins by an Expanded Genetic Code.
[So] Source:Chembiochem;18(18):1819-1823, 2017 Sep 19.
[Is] ISSN:1439-7633
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Marine mussels exhibit potent underwater adhesion abilities under hostile conditions by employing 3,4-dihydroxyphenylalanine (DOPA)-rich mussel adhesive proteins (MAPs). However, their recombinant production is a major biotechnological challenge. Herein, a novel strategy based on genetic code expansion has been developed by engineering efficient aminoacyl-transfer RNA synthetases (aaRSs) for the photocaged noncanonical amino acid ortho-nitrobenzyl DOPA (ONB-DOPA). The engineered ONB-DOPARS enables in vivo production of MAP type 5 site-specifically equipped with multiple instances of ONB-DOPA to yield photocaged, spatiotemporally controlled underwater adhesives. Upon exposure to UV light, these proteins feature elevated wet adhesion properties. This concept offers new perspectives for the production of recombinant bioadhesives.
[Mh] Termos MeSH primário: Bivalves/metabolismo
Código Genético/genética
Proteínas/metabolismo
[Mh] Termos MeSH secundário: Adesivos/efeitos da radiação
Aminoacil-tRNA Sintetases/metabolismo
Animais
Materiais Biomiméticos/metabolismo
Bivalves/genética
Di-Hidroxifenilalanina/metabolismo
Microscopia de Força Atômica
Microscopia de Varredura por Sonda
Mutagênese Sítio-Dirigida
Proteínas/genética
Proteínas Recombinantes/biossíntese
Proteínas Recombinantes/química
Proteínas Recombinantes/isolamento & purificação
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adhesives); 0 (Proteins); 0 (Recombinant Proteins); 0 (adhesive protein, mussel); 63-84-3 (Dihydroxyphenylalanine); EC 6.1.1.- (Amino Acyl-tRNA Synthetases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170627
[St] Status:MEDLINE
[do] DOI:10.1002/cbic.201700327


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[PMID]:28617398
[Au] Autor:Antonova IN; Goncharov VD; Bobrova EA
[Ad] Endereço:First Saint-Petersburg State Medical University named after I.P. Pavlov, Saint-Petersburg, Russia.
[Ti] Título:[Ultrastructural changes of human dental hard tissues during orthodontic treatment with fixed appliances].
[Ti] Título:Issledovanie ul'trastrukturnogo sostoianiia tverdykh tkanei zuba pri éksperimental'nom modelirovanii ortodonticheskogo lecheniia nes''emnoi apparaturoi..
[So] Source:Stomatologiia (Mosk);96(3):5-10, 2017.
[Is] ISSN:0039-1735
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The aim of the study was to evaluate ultrastructural changes of dental enamel after fixation of orthodontic appliances, initial influence of orthodontic forces and removal of braces. Five intact permanent tooth extracted for orthodontic reasons were included in the experimental study. Scanning probe microscopy was conducted in 4 random enamel points in each tooth (20 points overall) in semi-contact mode with standard 10 nm probes. The study showed ultrastructural enamel changes such as nanofractures up to 1 mm along the braces locks. The changes correlated with surface morphological features and teeth anatomy and may play an important role in dental decay and non-carious lesions occurring in the course of orthodontic treatment.
[Mh] Termos MeSH primário: Esmalte Dentário/ultraestrutura
Aparelhos Ortodônticos Removíveis/efeitos adversos
Braquetes Ortodônticos/efeitos adversos
[Mh] Termos MeSH secundário: Adolescente
Adulto
Cárie Dentária/etiologia
Dureza
Seres Humanos
Microscopia de Varredura por Sonda
Modelos Biológicos
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170810
[Lr] Data última revisão:
170810
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:170616
[St] Status:MEDLINE
[do] DOI:10.17116/stomat20179635-10


  3 / 544 MEDLINE  
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[PMID]:28258580
[Au] Autor:Solenov EI; Baturina GS; Katkova LE; Zarogiannis SG
[Ad] Endereço:Institute of Cytology and Genetics, SB RAS, 630090, Novosibirsk, Russia. eugsol@bionet.nsc.ru.
[Ti] Título:Methods to Measure Water Permeability.
[So] Source:Adv Exp Med Biol;969:263-276, 2017.
[Is] ISSN:0065-2598
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Water permeability is a key feature of the cell plasma membranes and it has seminal importance for a number of cell functions such as cell volume regulation, cell proliferation, cell migration, and angiogenesis to name a few. The transport of water occurs mainly through plasma membrane water channels , the aquaporins, who have very important function in physiological and pathophysiological states. Due to the above the experimental assessment of the water permeability of cells and tissues is necessary. The development of new methodologies of measuring water permeability is a vibrant scientific field that constantly develops during the past three decades along with the advances in imaging mainly. In this chapter we describe and critically assess several methods that have been developed for the measurement of water permeability both in living cells as well as in tissues with a focus in the first category.
[Mh] Termos MeSH primário: Aquaporinas/metabolismo
Microscopia de Força Atômica/métodos
Microscopia Eletroquímica de Varredura/métodos
Microscopia de Varredura por Sonda/métodos
Imagem Molecular/métodos
Água/metabolismo
[Mh] Termos MeSH secundário: Animais
Aquaporinas/genética
Membrana Celular/metabolismo
Permeabilidade da Membrana Celular
Movimento Celular
Proliferação Celular
Cães
Impedância Elétrica
Expressão Gênica
Seres Humanos
Células Madin Darby de Rim Canino
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Aquaporins); 059QF0KO0R (Water)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170305
[St] Status:MEDLINE
[do] DOI:10.1007/978-94-024-1057-0_18


  4 / 544 MEDLINE  
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[PMID]:28135335
[Au] Autor:Su T; Zhang H
[Ad] Endereço:State Key Laboratory of Surface Physics and Department of Physics, Fudan University, Shanghai, China.
[Ti] Título:Electrical Study of Trapped Charges in Copper-Doped Zinc Oxide Films by Scanning Probe Microscopy for Nonvolatile Memory Applications.
[So] Source:PLoS One;12(1):e0171050, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Charge trapping properties of electrons and holes in copper-doped zinc oxide (ZnO:Cu) films have been studied by scanning probe microscopy. We investigated the surface potential dependence on the voltage and duration applied to the copper-doped ZnO films by Kelvin probe force microscopy. It is found that the Fermi Level of the 8 at.% Cu-doped ZnO films shifted by 0.53 eV comparing to undoped ZnO films. This shift indicates significant change in the electronic structure and energy balance in Cu-doped ZnO films. The Fermi Level (work function) of zinc oxide films can be tuned by Cu doping, which are important for developing this functional material. In addition, Kelvin probe force microscopy measurements demonstrate that the nature of contact at Pt-coated tip/ZnO:Cu interface is changed from Schottky contact to Ohmic contact by increasing sufficient amount of Cu ions. The charge trapping property of the ZnO films enhance greatly by Cu doping (~10 at.%). The improved stable bipolar charge trapping properties indicate that copper-doped ZnO films are promising for nonvolatile memory applications.
[Mh] Termos MeSH primário: Cobre/química
Eletricidade
Microscopia de Varredura por Sonda/métodos
Óxido de Zinco/química
[Mh] Termos MeSH secundário: Microscopia de Força Atômica
Propriedades de Superfície
Volatilização
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
789U1901C5 (Copper); SOI2LOH54Z (Zinc Oxide)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170131
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0171050


  5 / 544 MEDLINE  
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[PMID]:28142317
[Au] Autor:Agrawal L; Sahu S; Ghosh S; Shiga T; Fujita D; Bandyopadhyay A
[Ad] Endereço:* National Institute for Materials Science (NIMS), 1-2-1 Sengen, Tsukuba, Japan.
[Ti] Título:Inventing atomic resolution scanning dielectric microscopy to see a single protein complex operation live at resonance in a neuron without touching or adulterating the cell.
[So] Source:J Integr Neurosci;15(4):435-462, 2016 Dec.
[Is] ISSN:0219-6352
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A substantial ion flow in a normally wet protein masks any other forms of signal transmission. We use hysteresis and linear conduction (both are artifacts) as a marker to precisely wet a protein, which restricts the ionic conduction (hysteresis disappears), and at the same time, it is not denatured (quantized conductance and Raman spectra are intact). Pure electric visualization of proteins at work by eliminating the screening of ions, electrons, would change the way we study biology. Here we discuss the technical challenges resolved for imaging a protein or live cell using nonlinear dielectric response (spatial distribution of conductance, capacitance and phase, GCP trio). We electromagnetically triggered electrical, mechanical, thermal and ionic resonant vibrations in a protein. During resonant oscillations, we imaged the protein using resonant scanning tunneling microscopy of biomaterials (Brestum) and during ionic firing we imaged live what happens inside an axon core of a neuron by using our atomic scale scanning dielectric microscopy (Asadim). Both Asadim and Brestum are housed in a homebuilt scanning tunneling microscope (bio-STM) and a special micro-grid developed by us (patent JP-5187804) for fractal supercomputing. We found the trick to turn a membrane transparent and see inside without making any physical contact. We image live that a protein molecule adopts a unique configuration for each resonance frequency, - thus far unknown to biology. "Membrane alone fires" is found to be wrong after a century, micro-neuro-filaments communicate prior to firing to decide its necessity and then regulate it suitably. We introduce a series of technologies e.g., fractal grid, point contact, micro THz antenna, to discover that from atomic structure to a living cell, the biomaterials vibrate collectively.
[Mh] Termos MeSH primário: Microscopia de Varredura por Sonda/instrumentação
Microscopia de Varredura por Sonda/métodos
Neurônios/metabolismo
[Mh] Termos MeSH secundário: Potenciais de Ação/fisiologia
Animais
Células Cultivadas
Fenômenos Eletromagnéticos
Desenho de Equipamento
Fractais
Hipocampo/metabolismo
Microeletrodos
Ratos
Tubulina (Proteína)/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Tubulin)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170228
[Lr] Data última revisão:
170228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170202
[St] Status:MEDLINE
[do] DOI:10.1142/S0219635216500333


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[PMID]:27768037
[Au] Autor:Ho MS; Huang CP; Tsai JH; Chou CF; Lee WJ
[Ad] Endereço:Department of Physics and Institute of Nanoscience, National Chung Hsing University.
[Ti] Título:Probing C84-embedded Si Substrate Using Scanning Probe Microscopy and Molecular Dynamics.
[So] Source:J Vis Exp;(115), 2016 Sep 28.
[Is] ISSN:1940-087X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This paper reports an array-designed C84-embedded Si substrate fabricated using a controlled self-assembly method in an ultra-high vacuum chamber. The characteristics of the C84-embedded Si surface, such as atomic resolution topography, local electronic density of states, band gap energy, field emission properties, nanomechanical stiffness, and surface magnetism, were examined using a variety of surface analysis techniques under ultra, high vacuum (UHV) conditions as well as in an atmospheric system. Experimental results demonstrate the high uniformity of the C84-embedded Si surface fabricated using a controlled self-assembly nanotechnology mechanism, represents an important development in the application of field emission display (FED), optoelectronic device fabrication, MEMS cutting tools, and in efforts to find a suitable replacement for carbide semiconductors. Molecular dynamics (MD) method with semi-empirical potential can be used to study the nanoindentation of C84-embedded Si substrate. A detailed description for performing MD simulation is presented here. Details for a comprehensive study on mechanical analysis of MD simulation such as indentation force, Young's modulus, surface stiffness, atomic stress, and atomic strain are included. The atomic stress and von-Mises strain distributions of the indentation model can be calculated to monitor deformation mechanism with time evaluation in atomistic level.
[Mh] Termos MeSH primário: Fulerenos/química
Microscopia de Varredura por Sonda/métodos
Silício/química
[Mh] Termos MeSH secundário: Módulo de Elasticidade
Simulação de Dinâmica Molecular
Nanotecnologia/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; VIDEO-AUDIO MEDIA
[Nm] Nome de substância:
0 (Fullerenes); Z4152N8IUI (Silicon)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170707
[Lr] Data última revisão:
170707
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161022
[St] Status:MEDLINE
[do] DOI:10.3791/54235


  7 / 544 MEDLINE  
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[PMID]:27269255
[Au] Autor:Roncati L; Gatti AM; Pusiol T; Piscioli F; Barbolini G; Maiorana A
[Ad] Endereço:a Department of Diagnostic and Clinical Medicine and of Public Health , University of Modena and Reggio Emilia , Modena , Italy.
[Ti] Título:The first investigative science-based evidence of Morgellons psychogenesis.
[So] Source:Ultrastruct Pathol;40(5):249-53, 2016 Sep-Oct.
[Is] ISSN:1521-0758
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Morgellons disease is an infrequent syndromic condition, that typically affects middle-aged white women, characterized by crawling sensations on and under the skin, associated with itchy rashes, stinging sores, fiber-like filaments emerging from the sores, severe fatigue, concentrating difficulty, and memory loss. The scientific community is prone to believe that Morgellons is the manifestation of various psychiatric syndromes (Munchausen, Munchausen by proxy, Ekbom, Wittmaack-Ekbom). Up until now, no investigative science-based evidence about its psychogenesis has ever been provided. In order to close this gap, we have analyzed the filaments extracted from the skin lesions of a 49-year-old Caucasian female patient, by using a Field Emission Gun-Environmental Electron Scanning Microscope equipped with an X-ray microprobe, for the chemico-elemental characterization of the filaments, comparing them with those collected during a detailed indoor investigation, with careful air monitoring, in her apartment. Our results prove the self-introduction under the epidermis of environmental filaments. For the first time in the literature, we have scientifically demonstrated the self-induced nature of Morgellons disease, thereby wiping out fanciful theories about its etiopathogenesis.
[Mh] Termos MeSH primário: Doença de Morgellons/psicologia
[Mh] Termos MeSH secundário: Feminino
Seres Humanos
Microscopia de Varredura por Sonda
Meia-Idade
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170330
[Lr] Data última revisão:
170330
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160609
[St] Status:MEDLINE
[do] DOI:10.1080/01913123.2016.1190434


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[PMID]:27224490
[Au] Autor:Shevchuk A; Tokar S; Gopal S; Sanchez-Alonso JL; Tarasov AI; Vélez-Ortega AC; Chiappini C; Rorsman P; Stevens MM; Gorelik J; Frolenkov GI; Klenerman D; Korchev YE
[Ad] Endereço:Department of Medicine, Imperial College London, London, United Kingdom. Electronic address: a.shevchuk@ic.ac.uk.
[Ti] Título:Angular Approach Scanning Ion Conductance Microscopy.
[So] Source:Biophys J;110(10):2252-65, 2016 05 24.
[Is] ISSN:1542-0086
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Scanning ion conductance microscopy (SICM) is a super-resolution live imaging technique that uses a glass nanopipette as an imaging probe to produce three-dimensional (3D) images of cell surface. SICM can be used to analyze cell morphology at nanoscale, follow membrane dynamics, precisely position an imaging nanopipette close to a structure of interest, and use it to obtain ion channel recordings or locally apply stimuli or drugs. Practical implementations of these SICM advantages, however, are often complicated due to the limitations of currently available SICM systems that inherited their design from other scanning probe microscopes in which the scan assembly is placed right above the specimen. Such arrangement makes the setting of optimal illumination necessary for phase contrast or the use of high magnification upright optics difficult. Here, we describe the designs that allow mounting SICM scan head on a standard patch-clamp micromanipulator and imaging the sample at an adjustable approach angle. This angle could be as shallow as the approach angle of a patch-clamp pipette between a water immersion objective and the specimen. Using this angular approach SICM, we obtained topographical images of cells grown on nontransparent nanoneedle arrays, of islets of Langerhans, and of hippocampal neurons under upright optical microscope. We also imaged previously inaccessible areas of cells such as the side surfaces of the hair cell stereocilia and the intercalated disks of isolated cardiac myocytes, and performed targeted patch-clamp recordings from the latter. Thus, our new, to our knowledge, angular approach SICM allows imaging of living cells on nontransparent substrates and a seamless integration with most patch-clamp setups on either inverted or upright microscopes, which would facilitate research in cell biophysics and physiology.
[Mh] Termos MeSH primário: Imagem Tridimensional/métodos
Microscopia de Varredura por Sonda/métodos
[Mh] Termos MeSH secundário: Adulto
Animais
Células Cultivadas
Meios de Cultura
Desenho de Equipamento
Feminino
Células HeLa
Seres Humanos
Imagem Tridimensional/instrumentação
Masculino
Camundongos
Micromanipulação/instrumentação
Micromanipulação/métodos
Microscopia Eletrônica de Varredura
Microscopia de Varredura por Sonda/instrumentação
Nanotecnologia
Técnicas de Patch-Clamp/instrumentação
Técnicas de Patch-Clamp/métodos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Culture Media)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171118
[Lr] Data última revisão:
171118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160526
[St] Status:MEDLINE


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[PMID]:27198224
[Au] Autor:Tsai YH; Bhandari DR; Garrett TJ; Carter CS; Spengler B; Yost RA
[Ad] Endereço:Department of Chemistry, University of Florida, Gainesville, FL, USA.
[Ti] Título:Skeletal muscle fiber analysis by atmospheric pressure scanning microprobe matrix-assisted laser desorption/ionization mass spectrometric imaging at high mass and high spatial resolution.
[So] Source:Proteomics;16(11-12):1822-4, 2016 Jun.
[Is] ISSN:1615-9861
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Skeletal muscles are composed of heterogeneous muscle fibers with various fiber types. These fibers can be classified into different classes based on their different characteristics. MALDI mass spectrometric imaging (MSI) has been applied to study and visualize different metabolomics profiles of different fiber types. Here, skeletal muscles were analyzed by atmospheric pressure scanning microprobe MALDI-MSI at high spatial and high mass resolution.
[Mh] Termos MeSH primário: Metabolômica/métodos
Fibras Musculares Esqueléticas/metabolismo
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
[Mh] Termos MeSH secundário: Animais
Pressão Atmosférica
Microscopia de Varredura por Sonda/métodos
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160521
[St] Status:MEDLINE
[do] DOI:10.1002/pmic.201500536


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[PMID]:27089378
[Au] Autor:Rauschenbach S; Ternes M; Harnau L; Kern K
[Ad] Endereço:Max-Planck-Institute for Solid State Research, D-70569 Stuttgart, Germany; email: s.rauschenbach@fkf.mpg.de.
[Ti] Título:Mass Spectrometry as a Preparative Tool for the Surface Science of Large Molecules.
[So] Source:Annu Rev Anal Chem (Palo Alto Calif);9(1):473-98, 2016 Jun 12.
[Is] ISSN:1936-1335
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Measuring and understanding the complexity that arises when nanostructures interact with their environment are one of the major current challenges of nanoscale science and technology. High-resolution microscopy methods such as scanning probe microscopy have the capacity to investigate nanoscale systems with ultimate precision, for which, however, atomic scale precise preparation methods of surface science are a necessity. Preparative mass spectrometry (pMS), defined as the controlled deposition of m/z filtered ion beams, with soft ionization sources links the world of large, biological molecules and surface science, enabling atomic scale chemical control of molecular deposition in ultrahigh vacuum (UHV). Here we explore the application of high-resolution scanning probe microscopy and spectroscopy to the characterization of structure and properties of large molecules. We introduce the fundamental principles of the combined experiments electrospray ion beam deposition and scanning tunneling microscopy. Examples for the deposition and investigation of single particles, for layer and film growth, and for the investigation of electronic properties of individual nonvolatile molecules show that state-of-the-art pMS technology provides a platform analog to thermal evaporation in conventional molecular beam epitaxy. Additionally, it offers additional, unique features due to the use of charged polyatomic particles. This new field is an enormous sandbox for novel molecular materials research and demands the development of advanced molecular ion beam technology.
[Mh] Termos MeSH primário: Fulerenos/análise
Substâncias Macromoleculares/análise
Espectrometria de Massas
Compostos Orgânicos/análise
Proteínas/análise
[Mh] Termos MeSH secundário: Microscopia de Varredura por Sonda
Microscopia de Tunelamento
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Fullerenes); 0 (Macromolecular Substances); 0 (Organic Chemicals); 0 (Proteins); NP9U26B839 (fullerene C60)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160419
[St] Status:MEDLINE
[do] DOI:10.1146/annurev-anchem-071015-041633



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