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Pesquisa : E01.370.350.700.810.810.900 [Categoria DeCS]
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[PMID]:29183290
[Au] Autor:Bajcsy P; Yoon S; Florczyk SJ; Hotaling NA; Simon M; Szczypinski PM; Schaub NJ; Simon CG; Brady M; Sriram RD
[Ad] Endereço:Information Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD, USA. peter.bajcsy@nist.gov.
[Ti] Título:Modeling, validation and verification of three-dimensional cell-scaffold contacts from terabyte-sized images.
[So] Source:BMC Bioinformatics;18(1):526, 2017 Nov 28.
[Is] ISSN:1471-2105
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Cell-scaffold contact measurements are derived from pairs of co-registered volumetric fluorescent confocal laser scanning microscopy (CLSM) images (z-stacks) of stained cells and three types of scaffolds (i.e., spun coat, large microfiber, and medium microfiber). Our analysis of the acquired terabyte-sized collection is motivated by the need to understand the nature of the shape dimensionality (1D vs 2D vs 3D) of cell-scaffold interactions relevant to tissue engineers that grow cells on biomaterial scaffolds. RESULTS: We designed five statistical and three geometrical contact models, and then down-selected them to one from each category using a validation approach based on physically orthogonal measurements to CLSM. The two selected models were applied to 414 z-stacks with three scaffold types and all contact results were visually verified. A planar geometrical model for the spun coat scaffold type was validated from atomic force microscopy images by computing surface roughness of 52.35 nm ±31.76 nm which was 2 to 8 times smaller than the CLSM resolution. A cylindrical model for fiber scaffolds was validated from multi-view 2D scanning electron microscopy (SEM) images. The fiber scaffold segmentation error was assessed by comparing fiber diameters from SEM and CLSM to be between 0.46% to 3.8% of the SEM reference values. For contact verification, we constructed a web-based visual verification system with 414 pairs of images with cells and their segmentation results, and with 4968 movies with animated cell, scaffold, and contact overlays. Based on visual verification by three experts, we report the accuracy of cell segmentation to be 96.4% with 94.3% precision, and the accuracy of cell-scaffold contact for a statistical model to be 62.6% with 76.7% precision and for a geometrical model to be 93.5% with 87.6% precision. CONCLUSIONS: The novelty of our approach lies in (1) representing cell-scaffold contact sites with statistical intensity and geometrical shape models, (2) designing a methodology for validating 3D geometrical contact models and (3) devising a mechanism for visual verification of hundreds of 3D measurements. The raw and processed data are publicly available from https://isg.nist.gov/deepzoomweb/data/ together with the web -based verification system.
[Mh] Termos MeSH primário: Imagem Tridimensional/métodos
Modelos Biológicos
Tecidos Suporte/química
[Mh] Termos MeSH secundário: Algoritmos
Materiais Biocompatíveis/química
Células da Medula Óssea/citologia
Seres Humanos
Internet
Masculino
Células Mesenquimais Estromais/citologia
Microscopia de Força Atômica
Microscopia Confocal
Microscopia Eletrônica de Varredura
Interface Usuário-Computador
Microtomografia por Raio-X
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1186/s12859-017-1928-x


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[PMID]:29292412
[Au] Autor:Al-Nuaimi N; Patel S; Foschi F; Mannocci F; Austin RS
[Ti] Título:Assessment of residual coronal tooth structure postendodontic cavity preparation using digital dental impressions and micro-computed tomography.
[So] Source:Int J Comput Dent;20(4):377-392, 2017.
[Is] ISSN:1463-4201
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: To evaluate the in vitro accuracy of digital impressions for three-dimensional (3D) volumetric measurement of residual coronal tooth structure postendodontic cavity preparation, with reference to micro-computed tomography (µCT). METHODS: Quantification of the accuracy and precision of the intraoral digital scanner (3M True Definition Scanner - IOS) was performed using a metrology gauge block and a profilometric calibration model. Thirty-four human extracted molars with endodontic access cavities were scanned using both intraoral scanning (test scanner) in high-resolution mode, and µCT (reference scanner: GE Locus SP µCT scanner) in high- (HiResCT) and low- (LoResCT) resolution modes. Comparisons of volumetric accuracy and 3D profilometric deviations were performed using surface metrology software. One-way repeated measures analysis of variance (ANOVA), in combination with the Bonferroni post hoc test, was implemented to compare the differences in volume measurements between scanning methods. RESULTS: Digital scanning revealed smaller volume measurements by 1.36% and 0.68% compared to HiResCT and LoResCT, respectively. There was a statistically significant difference in the volumetric measurements obtained from the IOS scanner and both HiResCT and LoResCT scans (P < 0.001). Analysis of the mean 3D profilometric deviations revealed that the IOS displayed greater surface deviation (± 27/33 µm) vs HiResCT and LoResCT (± 16/32 µm). CONCLUSIONS: Although volumetric measurements of endodontically accessed teeth were up to 1.36% smaller in comparison to µCT, the digital scanner was able to reliably measure the extra- and intracoronal aspect of the endodontically accessed tooth.
[Mh] Termos MeSH primário: Técnica de Moldagem Odontológica
Processamento de Imagem Assistida por Computador
Dente não Vital
Microtomografia por Raio-X
[Mh] Termos MeSH secundário: Modelos Dentários
Seres Humanos
Imagem Tridimensional
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE


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[PMID]:29343708
[Au] Autor:Peck VL; Oakes RL; Harper EM; Manno C; Tarling GA
[Ad] Endereço:British Antarctic Survey, Natural Environment Research Council, High Cross, Madingley Road, Cambridge, CB3 0ET, UK. vlp@bas.ac.uk.
[Ti] Título:Pteropods counter mechanical damage and dissolution through extensive shell repair.
[So] Source:Nat Commun;9(1):264, 2018 01 17.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The dissolution of the delicate shells of sea butterflies, or pteropods, has epitomised discussions regarding ecosystem vulnerability to ocean acidification over the last decade. However, a recent demonstration that the organic coating of the shell, the periostracum, is effective in inhibiting dissolution suggests that pteropod shells may not be as susceptible to ocean acidification as previously thought. Here we use micro-CT technology to show how, despite losing the entire thickness of the original shell in localised areas, specimens of polar species Limacina helicina maintain shell integrity by thickening the inner shell wall. One specimen collected within Fram Strait with a history of mechanical and dissolution damage generated four times the thickness of the original shell in repair material. The ability of pteropods to repair and maintain their shells, despite progressive loss, demonstrates a further resilience of these organisms to ocean acidification but at a likely metabolic cost.
[Mh] Termos MeSH primário: Exoesqueleto/fisiologia
Ecossistema
Gastrópodes/fisiologia
Estresse Mecânico
[Mh] Termos MeSH secundário: Exoesqueleto/anatomia & histologia
Exoesqueleto/ultraestrutura
Animais
Gastrópodes/anatomia & histologia
Gastrópodes/ultraestrutura
Concentração de Íons de Hidrogênio
Microscopia Eletrônica de Varredura
Água do Mar/química
Microtomografia por Raio-X
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180119
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02692-w


  4 / 5792 MEDLINE  
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[PMID]:29211289
[Au] Autor:Queiroz PM; Rovaris K; Santaella GM; Haiter-Neto F; Freitas DQ
[Ad] Endereço:Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba, Departamento de Diagnóstico Oral, Área de Radiologia Oral, Piracicaba, SP, Brasil.
[Ti] Título:Comparison of automatic and visual methods used for image segmentation in Endodontics: a microCT study.
[So] Source:J Appl Oral Sci;25(6):674-679, 2017 Nov-Dec.
[Is] ISSN:1678-7765
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To calculate root canal volume and surface area in microCT images, an image segmentation by selecting threshold values is required, which can be determined by visual or automatic methods. Visual determination is influenced by the operator's visual acuity, while the automatic method is done entirely by computer algorithms. To compare between visual and automatic segmentation, and to determine the influence of the operator's visual acuity on the reproducibility of root canal volume and area measurements. MATERIAL AND METHODS: Images from 31 extracted human anterior teeth were scanned with a µCT scanner. Three experienced examiners performed visual image segmentation, and threshold values were recorded. Automatic segmentation was done using the "Automatic Threshold Tool" available in the dedicated software provided by the scanner's manufacturer. Volume and area measurements were performed using the threshold values determined both visually and automatically. RESULTS: The paired Student's t-test showed no significant difference between visual and automatic segmentation methods regarding root canal volume measurements (p=0.93) and root canal surface (p=0.79). CONCLUSION: Although visual and automatic segmentation methods can be used to determine the threshold and calculate root canal volume and surface, the automatic method may be the most suitable for ensuring the reproducibility of threshold determination.
[Mh] Termos MeSH primário: Cavidade Pulpar/diagnóstico por imagem
Endodontia
Microtomografia por Raio-X
[Mh] Termos MeSH secundário: Algoritmos
Seres Humanos
Processamento de Imagem Assistida por Computador/métodos
Imagem Tridimensional/métodos
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE


  5 / 5792 MEDLINE  
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[PMID]:29211280
[Au] Autor:Vieira AEM; Danelon M; Camara DMD; Rosselli ER; Stock SR; Cannon ML; Xiao X; De Carlo F; Delbem ACB
[Ad] Endereço:Univ. Estadual Paulista, Faculdade de Odontologia de Araçatuba, Departamento de Odontologia Infantil e Social, Araçatuba, SP, Brasil.
[Ti] Título:In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization.
[So] Source:J Appl Oral Sci;25(6):596-603, 2017 Nov-Dec.
[Is] ISSN:1678-7765
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). MATERIAL AND METHODS: Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (%SHR) and to calculate mineral concentration (gHAp.cm-3), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<0.05). RESULTS: Fluoride gel presented higher %SHR followed by ACP 3 min (p<0.001). No difference (p = 0.148) was found for Placebo, ACP 3 h and ACP 8 h groups for %SHR. Fluoride gel showed greater mineral concentration (p<0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. CONCLUSION: In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process.
[Mh] Termos MeSH primário: Caseínas/farmacologia
Esmalte Dentário/efeitos dos fármacos
Remineralização Dentária
[Mh] Termos MeSH secundário: Animais
Bovinos
Esmalte Dentário/diagnóstico por imagem
Técnicas In Vitro
Síncrotrons
Fatores de Tempo
Microtomografia por Raio-X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Caseins); 0 (casein phosphopeptide-amorphous calcium phosphate nanocomplex)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE


  6 / 5792 MEDLINE  
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[PMID]:29352303
[Au] Autor:Gerli MFM; Guyette JP; Evangelista-Leite D; Ghoshhajra BB; Ott HC
[Ad] Endereço:Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, United States of America.
[Ti] Título:Perfusion decellularization of a human limb: A novel platform for composite tissue engineering and reconstructive surgery.
[So] Source:PLoS One;13(1):e0191497, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Muscle and fasciocutaneous flaps taken from autologous donor sites are currently the most utilized approach for trauma repair, accounting annually for 4.5 million procedures in the US alone. However, the donor tissue size is limited and the complications related to these surgical techniques lead to morbidities, often involving the donor sites. Alternatively, recent reports indicated that extracellular matrix (ECM) scaffolds boost the regenerative potential of the injured site, as shown in a small cohort of volumetric muscle loss patients. Perfusion decellularization is a bioengineering technology that allows the generation of clinical-scale ECM scaffolds with preserved complex architecture and with an intact vascular template, from a variety of donor organs and tissues. We recently reported that this technology is amenable to generate full composite tissue scaffolds from rat and non-human primate limbs. Translating this platform to human extremities could substantially benefit soft tissue and volumetric muscle loss patients providing tissue- and species-specific grafts. In this proof-of-concept study, we show the successful generation a large-scale, acellular composite tissue scaffold from a full cadaveric human upper extremity. This construct retained its morphological architecture and perfusable vascular conduits. Histological and biochemical validation confirmed the successful removal of nuclear and cellular components, and highlighted the preservation of the native extracellular matrix components. Our results indicate that perfusion decellularization can be applied to produce human composite tissue acellular scaffolds. With its preserved structure and vascular template, these biocompatible constructs, could have significant advantages over the currently implanted matrices by means of nutrient distribution, size-scalability and immunological response.
[Mh] Termos MeSH primário: Braço/cirurgia
Procedimentos Cirúrgicos Reconstrutivos/métodos
Engenharia Tecidual/métodos
Tecidos Suporte
[Mh] Termos MeSH secundário: Animais
Braço/anatomia & histologia
Braço/irrigação sanguínea
Reatores Biológicos
Cadáver
Matriz Extracelular/química
Seres Humanos
Imagem Tridimensional
Masculino
Meia-Idade
Perfusão
Ratos
Engenharia Tecidual/instrumentação
Tecidos Suporte/química
Microtomografia por Raio-X
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180121
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191497


  7 / 5792 MEDLINE  
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[PMID]:29304115
[Au] Autor:Ando K; Imagama S; Kobayashi K; Ito K; Tsushima M; Morozumi M; Tanaka S; Machino M; Ota K; Nishida K; Nishida Y; Ishiguro N
[Ad] Endereço:Department of Orthopedic Surgery, Nagoya University Graduate School of Medicine, Showa-ku, Nagoya, Aichi, Japan.
[Ti] Título:Effects of a self-assembling peptide as a scaffold on bone formation in a defect.
[So] Source:PLoS One;13(1):e0190833, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Spinal fusion and bone defect after injuries, removal of bone tumors, and infections need to be repaired by implantation. In an aging society, recovery from these procedures is often difficult. In this study, we found that injection of SPG-178 leads to expression of several bone marker genes and mineralization in vitro, and revealed a significantly higher degree of newly formed bone matrix with use of SPG-178 in vivo. MC3T3-E1 cells were used to evaluate osteoblast differentiation promoted by SPG-178. To analyze gene expression, total RNA was isolated from MC3T3-E1 cells cultured for 7 and 14 days with control medium or SPG-178 medium. Among the several bone marker genes examined, SPG-178 significantly increased the mRNA levels for ALP, BMP-2 and Osteocalcin, OPN, BSP and for the Osterix. Ten-week-old female Wistar rats were used for all transplantation procedures. A PEEK cage was implanted into a bony defect (5 mm) within the left femoral mid-shaft, and stability was maintained by an external fixator. The PEEK cages were filled with either a SPG-178 hydrogel plus allogeneic bone chips (n = 4) or only allogeneic bone chips (n = 4). The rats were then kept for 56 days. Newly formed bone matrix was revealed inside the PEEK cage and there was an increased bone volume per total volume with the cage filled with SPG-178, compared to the control group. SPG-178 has potential in clinical applications because it has several benefits. These include its favorable bone conduction properties its ability to act as a support for various different cells and growth factors, its lack of infection risk compared with materials of animal origin such as ECM, and the ease with which it can be used to fill defects with complex shapes and combined with a wide range of other materials.
[Mh] Termos MeSH primário: Desenvolvimento Ósseo
Peptídeos/metabolismo
Tecidos Suporte
[Mh] Termos MeSH secundário: Células 3T3
Animais
Calcificação Fisiológica
Linhagem Celular
Feminino
Camundongos
Camundongos Endogâmicos C57BL
Ratos
Ratos Wistar
Reação em Cadeia da Polimerase em Tempo Real
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Microtomografia por Raio-X
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Peptides)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190833


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[PMID]:29304096
[Au] Autor:Gravesen E; Lerche Mace M; Nordholm A; Hofman-Bang J; Hruska K; Haagen Nielsen C; Kjær A; Olgaard K; Lewin E
[Ad] Endereço:Department of Clinical Medicine, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark.
[Ti] Título:Exogenous BMP7 in aortae of rats with chronic uremia ameliorates expression of profibrotic genes, but does not reverse established vascular calcification.
[So] Source:PLoS One;13(1):e0190820, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hyperphosphatemia and vascular calcification are frequent complications of chronic renal failure and bone morphogenetic protein 7 (BMP7) has been shown to protect against development of vascular calcification in uremia. The present investigation examined the potential reversibility of established uremic vascular calcification by treatment of uremic rats with BMP7. A control model of isogenic transplantation of a calcified aorta from uremic rats into healthy littermates examined whether normalization of the uremic environment reversed vascular calcification. Uremia and vascular calcification were induced in rats by 5/6 nephrectomy, high phosphate diet and alfacalcidol treatment. After 14 weeks severe vascular calcification was present and rats were allocated to BMP7, vehicle or aorta transplantation. BMP7 treatment caused a significant decrease of plasma phosphate to 1.56 ± 0.17 mmol/L vs 2.06 ± 0.34 mmol/L in the vehicle group even in the setting of uremia and high phosphate diet. Uremia and alfacalcidol resulted in an increase in aortic expression of genes related to fibrosis, osteogenic transformation and extracellular matrix calcification, and the BMP7 treatment resulted in a decrease in the expression of profibrotic genes. The total Ca-content of the aorta was however unchanged both in the abdominal aorta: 1.9 ± 0.6 µg/mg tissue in the vehicle group vs 2.2 ± 0.6 µg/mg tissue in the BMP7 group and in the thoracic aorta: 71 ± 27 µg/mg tissue in the vehicle group vs 54 ± 18 µg/mg tissue in the BMP7 group. Likewise, normalization of the uremic environment by aorta transplantation had no effect on the Ca-content of the calcified aorta: 16.3 ± 0.6 µg/mg tissue pre-transplantation vs 15.9 ± 2.3 µg/mg tissue post-transplantation. Aortic expression of genes directly linked to extracellular matrix calcification was not affected by BMP7 treatment, which hypothetically might explain persistent high Ca-content in established vascular calcification. The present results highlight the importance of preventing the development of vascular calcification in chronic kidney disease. Once established, vascular calcification persists even in the setting when hyperphosphatemia or the uremic milieu is abolished.
[Mh] Termos MeSH primário: Proteína Morfogenética Óssea 7/farmacologia
Regulação da Expressão Gênica/efeitos dos fármacos
Uremia/tratamento farmacológico
Calcificação Vascular/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Aorta/efeitos dos fármacos
Aorta/metabolismo
Proteína Morfogenética Óssea 7/uso terapêutico
Doença Crônica
Fibrose
Masculino
Fosfatos/sangue
Ratos
Reação em Cadeia da Polimerase em Tempo Real
Uremia/genética
Microtomografia por Raio-X
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bone Morphogenetic Protein 7); 0 (Phosphates)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190820


  9 / 5792 MEDLINE  
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[PMID]:29304080
[Au] Autor:Hong C; Quach A; Lin L; Olson J; Kwon T; Bezouglaia O; Tran J; Hoang M; Bui K; Kim RH; Tetradis S
[Ad] Endereço:Section of Orthodontics, Division of Growth and Development, UCLA School of Dentistry, Los Angeles, California, United States of America.
[Ti] Título:Local vs. systemic administration of bisphosphonates in rat cleft bone graft: A comparative study.
[So] Source:PLoS One;13(1):e0190901, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A majority of patients with orofacial cleft deformity requires cleft repair through a bone graft. However, elevated amount of bone resorption and subsequent bone graft failure remains a significant clinical challenge. Bisphosphonates (BPs), a class of anti-resorptive drugs, may offer great promise in enhancing the clinical success of bone grafting. In this study, we compared the effects of systemic and local delivery of BPs in an intraoral bone graft model in rats. We randomly divided 34 female 20-week-old Fischer F344 Inbred rats into four groups to repair an intraoral critical-sized defect (CSD): (1) Control: CSD without graft (n = 4); (2) Graft/Saline: bone graft with systemic administration of saline 1 week post-operatively (n = 10); (3) Graft/Systemic: bone graft with systemic administration of zoledronic acid 1 week post-operatively (n = 10); and (4) Graft/Local: bone graft pre-treated with zoledronic acid (n = 10). At 6-weeks post-operatively, microCT volumetric analysis showed a significant increase in bone fraction volume (BV/TV) in the Graft/Systemic (62.99 ±14.31%) and Graft/Local (69.35 ±13.18%) groups compared to the Graft/Saline (39.18±10.18%). Similarly, histological analysis demonstrated a significant increase in bone volume in the Graft/Systemic (78.76 ±18.00%) and Graft/Local (89.95 ±4.93%) groups compared to the Graft/Saline (19.74±18.89%). The local delivery approach resulted in the clinical success of bone grafts, with reduced graft resorption and enhanced osteogenesis and bony integration with defect margins while avoiding the effects of BPs on peripheral osteoclastic function. In addition, local delivery of BPs may be superior to systemic delivery with its ease of procedure as it involves simple soaking of bone graft materials in BP solution prior to graft placement into the defect. This new approach may provide convenient and promising clinical applications towards effectively managing cleft patients.
[Mh] Termos MeSH primário: Transplante Ósseo
Fissura Palatina/cirurgia
Difosfonatos/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Fissura Palatina/tratamento farmacológico
Feminino
Osteoclastos/efeitos dos fármacos
Ratos
Ratos Endogâmicos F344
Microtomografia por Raio-X
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Diphosphonates)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190901


  10 / 5792 MEDLINE  
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[PMID]:29370213
[Au] Autor:Borg SA; Buckley H; Owen R; Marin AC; Lu Y; Eyles D; Lacroix D; Reilly GC; Skerry TM; Bishop NJ
[Ad] Endereço:Academic Unit of Child Health Department of Oncology & Metabolism, University of Sheffield, Sheffield, United Kingdom.
[Ti] Título:Early life vitamin D depletion alters the postnatal response to skeletal loading in growing and mature bone.
[So] Source:PLoS One;13(1):e0190675, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:There is increasing evidence of persistent effects of early life vitamin D exposure on later skeletal health; linking low levels in early life to smaller bone size in childhood as well as increased fracture risk later in adulthood, independently of later vitamin D status. A major determinant of bone mass acquisition across all ages is mechanical loading. We tested the hypothesis in an animal model system that early life vitamin D depletion results in abrogation of the response to mechanical loading, with consequent reduction in bone size, mass and strength during both childhood and adulthood. A murine model was created in which pregnant dams were either vitamin D deficient or replete, and their offspring moved to a vitamin D replete diet at weaning. Tibias of the offspring were mechanically loaded and bone structure, extrinsic strength and growth measured both during growth and after skeletal maturity. Offspring of vitamin D deplete mice demonstrated lower bone mass in the non loaded limb and reduced bone mass accrual in response to loading in both the growing skeleton and after skeletal maturity. Early life vitamin D depletion led to reduced bone strength and altered bone biomechanical properties. These findings suggest early life vitamin D status may, in part, determine the propensity to osteoporosis and fracture that blights later life in many individuals.
[Mh] Termos MeSH primário: Desenvolvimento Ósseo
Osso e Ossos/fisiopatologia
Deficiência de Vitamina D/fisiopatologia
[Mh] Termos MeSH secundário: Animais
Densidade Óssea
Feminino
Análise de Elementos Finitos
Seres Humanos
Camundongos
Camundongos Endogâmicos C57BL
Gravidez
Estresse Mecânico
Vitamina D/administração & dosagem
Microtomografia por Raio-X
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
1406-16-2 (Vitamin D)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190675



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