Base de dados : MEDLINE
Pesquisa : E01.370.376.550.650.650 [Categoria DeCS]
Referências encontradas : 100 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 10 ir para página                        

  1 / 100 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:27298564
[Au] Autor:Zhou XY; Li M; Li X; Long X; Zuo XL; Hou XH; Cong YZ; Li YQ
[Ad] Endereço:Xiao-Yan Zhou, Ming Li, Xia Li, Xin Long, Xiu-Li Zuo, Yan-Qing Li, Department of Gastroenterology, Shandong University, Qilu Hospital, Jinan 250012, Shandong Province, China.
[Ti] Título:Visceral hypersensitive rats share common dysbiosis features with irritable bowel syndrome patients.
[So] Source:World J Gastroenterol;22(22):5211-27, 2016 Jun 14.
[Is] ISSN:2219-2840
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:AIM: To evaluate gut microbial dysbiosis in two visceral hypersensitive models in comparison with irritable bowel syndrome (IBS) patients and to explore the extent to which these models capture the dysbiosis of IBS patients. METHODS: Visceral hypersensitivity was developed using the maternal separation (MS) rat model and post-inflammatory rat model. The visceral sensitivity of the model groups and control group was evaluated using the abdominal withdraw reflex score and electromyography in response to graded colorectal distention. The 16S ribosomal RNA gene from fecal samples was pyrosequenced and analyzed. The correlation between dysbiosis in the microbiota and visceral hypersensitivity was calculated. Positive findings were compared to sequencing data from a published human IBS cohort. RESULTS: Dysbiosis triggered by neonatal maternal separation was lasting but not static. Both MS and post-inflammatory rat fecal microbiota deviated from that of the control rats to an extent that was larger than the co-housing effect. Two short chain fatty acid producing genera, Fusobacterium and Clostridium XI, were shared by the human IBS cohort and by the maternal separation rats and post-inflammatory rats, respectively, to different extents. Fusobacterium was significantly increased in the MS group, and its abundance positively correlated with the degree of visceral hypersensitivity. Porphyromonadaceae was a protective biomarker for both the rat control group and healthy human controls. CONCLUSION: The dysbiosis MS rat model and the post-inflammatory rat model captured some of the dysbiosis features of IBS patients. Fusobacterium, Clostridium XI and Porphyromonadaceae were identified as targets for future mechanistic research.
[Mh] Termos MeSH primário: Colo/inervação
Disbiose
Microbioma Gastrointestinal
Trato Gastrointestinal/microbiologia
Hiperalgesia/microbiologia
Síndrome do Intestino Irritável/microbiologia
[Mh] Termos MeSH secundário: Animais
Clostridium/classificação
Modelos Animais de Doenças
Eletromiografia
Fezes/microbiologia
Fusobactérias/classificação
Seres Humanos
Hiperalgesia/fisiopatologia
Síndrome do Intestino Irritável/diagnóstico
Síndrome do Intestino Irritável/fisiopatologia
Mecanotransdução Celular
Percepção da Dor
Filogenia
Pressão
Ratos Sprague-Dawley
Reto/inervação
Reflexo Abdominal
Reflexo Anormal
Ribotipagem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170410
[Lr] Data última revisão:
170410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160615
[St] Status:MEDLINE
[do] DOI:10.3748/wjg.v22.i22.5211


  2 / 100 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26518368
[Au] Autor:Müller J; Müller S; Engel T; Reschke A; Baur H; Mayer F
[Ad] Endereço:University Outpatient Clinic, Sports Medicine & Sports Orthopaedics, University of Potsdam, Germany. Electronic address: thormei@uni-potsdam.de.
[Ti] Título:Stumbling reactions during perturbed walking: Neuromuscular reflex activity and 3-D kinematics of the trunk - A pilot study.
[So] Source:J Biomech;49(6):933-938, 2016 Apr 11.
[Is] ISSN:1873-2380
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Reflex activity of the lower leg muscles involved when compensating for falls has already been thoroughly investigated. However, the trunk׳s role in this compensation strategy remains unclear. The purpose of this study, therefore, was to analyze the kinematics and muscle activity of the trunk during perturbed walking. Ten subjects (29 ± 3 yr;79 ± 11 cm;74 ± 14 kg) walked (1m/s) on a split-belt treadmill, while 5 randomly timed, right-sided perturbations (treadmill belt deceleration: 40 m/s(2)) were applied. Trunk muscle activity was assessed with a 12-lead-EMG. Trunk kinematics were measured with a 3D-motion analysis system (12 markers framing 3 segments: upper thoracic area (UTA), lower thoracic area (LTA), lumbar area (LA)). The EMG-RMS [%] (0-200 ms after perturbation) was analyzed and then normalized to the RMS of normal walking. The total range of motion (ROM;[°]) for the extension/flexion, lateral flexion and rotation of each segment were calculated. Individual kinematic differences between walking and stumbling [%; ROM] were also computed. Data analysis was conducted descriptively, followed by one- and two-way ANOVAs (α=0.05). Stumbling led to an increase in ROM, compared to unperturbed gait, in all segments and planes. These increases ranged between 107 ± 26% (UTA/rotation) and 262 ± 132% (UTS/lateral flexion), significant only in lateral flexion. EMG activity of the trunk was increased during stumbling (abdominal: 665 ± 283%; back: 501 ± 215%), without significant differences between muscles. Provoked stumbling leads to a measurable effect on the trunk, quantifiable by an increase in ROM and EMG activity, compared to normal walking. Greater abdominal muscle activity and ROM of lateral flexion may indicate a specific compensation pattern occurring during stumbling.
[Mh] Termos MeSH primário: Acidentes por Quedas/prevenção & controle
Caminhada/fisiologia
[Mh] Termos MeSH secundário: Adulto
Dorso/fisiologia
Fenômenos Biomecânicos
Feminino
Marcha/fisiologia
Seres Humanos
Masculino
Músculo Esquelético/fisiologia
Projetos Piloto
Equilíbrio Postural
Desempenho Psicomotor
Amplitude de Movimento Articular/fisiologia
Reflexo Abdominal
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151101
[St] Status:MEDLINE


  3 / 100 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25768074
[Au] Autor:Ho J; Richardson JK
[Ad] Endereço:From the Department of Physical Medicine and Rehabilitation, University of Michigan, Ann Arbor, Michigan.
[Ti] Título:Rectus abdominis denervation after subcostal open laparotomy.
[So] Source:Am J Phys Med Rehabil;94(5):e43-4, 2015 May.
[Is] ISSN:1537-7385
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Nervos Intercostais/lesões
Laparotomia/efeitos adversos
Paralisia/etiologia
Reto do Abdome/inervação
[Mh] Termos MeSH secundário: Seres Humanos
Masculino
Meia-Idade
Denervação Muscular
Reflexo Abdominal
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1506
[Cu] Atualização por classe:161018
[Lr] Data última revisão:
161018
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:150314
[St] Status:MEDLINE
[do] DOI:10.1097/PHM.0000000000000256


  4 / 100 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:24785227
[Au] Autor:Gosavi TD; Lo YL
[Ad] Endereço:National Neuroscience Institute, Singapore, Singapore tgosavi@hotmail.com.
[Ti] Título:Images in clinical medicine. Superficial abdominal reflex.
[So] Source:N Engl J Med;370(18):e29, 2014 May 01.
[Is] ISSN:1533-4406
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Neuromielite Óptica/diagnóstico
Reflexo Abdominal/fisiologia
Reflexo Anormal/fisiologia
Medula Espinal/patologia
[Mh] Termos MeSH secundário: Anticorpos/análise
Aquaporina 4/imunologia
Seres Humanos
Imagem por Ressonância Magnética
Masculino
Meia-Idade
Neuromielite Óptica/fisiopatologia
Vértebras Torácicas
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (AQP4 protein, human); 0 (Antibodies); 0 (Aquaporin 4)
[Em] Mês de entrada:1405
[Cu] Atualização por classe:140505
[Lr] Data última revisão:
140505
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:140503
[St] Status:MEDLINE
[do] DOI:10.1056/NEJMicm1308153


  5 / 100 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:24695995
[Au] Autor:Boes CJ
[Ad] Endereço:Department of Neurology, Mayo Clinic, Rochester, MN, 55905, USA, boes.christopher@mayo.edu.
[Ti] Título:The history of examination of reflexes.
[So] Source:J Neurol;261(12):2264-74, 2014 Dec.
[Is] ISSN:1432-1459
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In the late 1800s, Wilhelm Erb, Joseph Babinski, William Gowers, and others helped develop the neurologic examination as we know it today. Erb was one of the first to emphasize a detailed and systematic neurologic exam and was co-discoverer of the muscle stretch reflex, Gowers began studying the knee jerk shortly after it was described, and Babinski focused on finding reliable signs that could differentiate organic from hysterical paralysis. These physicians and others emphasized the bedside examination of reflexes, which have been an important part of the neurologic examination ever since. This review will focus on the history of the examination of the following muscle stretch and superficial/cutaneous reflexes: knee jerk, jaw jerk, deep abdominal reflexes, superficial abdominal reflexes, plantar reflex/Babinski sign, and palmomental reflex. The history of reflex grading will also be discussed.
[Mh] Termos MeSH primário: Exame Neurológico/história
[Mh] Termos MeSH secundário: História do Século XIX
História do Século XX
História do Século XXI
Seres Humanos
Reflexo/fisiologia
Reflexo Abdominal/fisiologia
Reflexo Anormal/fisiologia
Reflexo de Babinski/fisiologia
Reflexo de Estiramento/fisiologia
[Pt] Tipo de publicação:HISTORICAL ARTICLE; JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1509
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140404
[St] Status:MEDLINE
[do] DOI:10.1007/s00415-014-7326-7


  6 / 100 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:24475678
[Au] Autor:Dhadke SV; Dhadke VN; Bangar SS; Korade MB
[Ad] Endereço:Dept. of Medicine, Dr. V.M. Govt. Medical College, Solapur.
[Ti] Título:Clinical profile of Guillain Barre syndrome.
[So] Source:J Assoc Physicians India;61(3):168-72, 2013 Mar.
[Is] ISSN:0004-5772
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: To study clinical presentation, hospital care and outcome of patients of Guillain Barre Syndrome (GBS) and number of patients of respiratory failure and need for ventilators. To study efficacy of IVIg in patients of GBS. MATERIAL AND METHODS: 40 patients of GBS studied in detail including history, clinical examination and investigations (Nerve conduction velocity and C.S.F. examination). All patients were watched for respiratory insufficiency and those who developed respiratory paralysis were given assisted mechanical ventilation. Patients were treated with IVIG and outcome was observed. Outcome of 2 groups of patients one treated with IVIg and other not treated with IVIg (supportive line of treatment) were compared. RESULTS: Commonest age group affected was 13-40 yrs. The male:female ratio was 1.5:1. Antecedent infection in form of fever, cough [11 patients], loose motions [10 patients] were present in 21 out of 40 patients. Quadriparesis was present in 39 patients and paraparesis in 1 patient. Cranial nerve involvement was seen in 25 out of 40 patients. Facial nerve was involved in 12 [30%] patients and Glossopharyengeal, vagus nerves were involved in 12 [30%] patients. Areflexia was found in all 40 patients. In CSF examination, albuminocytologic dissociation was present in 17 out of 26 patients. NCV findings show conduction velocity slowing, delayed f latencies in 90% patients. Out of 40 patients, 13 [30%] required mechanical ventilation. Out of 40 patients, 14 were treated with IVIg, 4 patients treated with plasmapheresis and 22 patients received only supportive treatment. Out of 40 patients 30 [75%] patients recovered completely, 8 [20%] patients died and 2 [5%] patients developed severe neurologic deficit. CONCLUSION: GBS is more common in 13-40 yrs age group with male:female ratio of 1.5:1. Antecedent infection is seen in 55% patients. Commonest presentation was paresthesia in legs and ascending paralysis. One third [32.5%] patients developed respiratory paralysis and needed ventilatory support. Patients who received IVIg early in the course of disease had faster recovery as compared to patients who received only supportive line of treatment.
[Mh] Termos MeSH primário: Síndrome de Guillain-Barré/diagnóstico
Síndrome de Guillain-Barré/terapia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Nervos Cranianos/fisiopatologia
Feminino
Síndrome de Guillain-Barré/complicações
Síndrome de Guillain-Barré/fisiopatologia
Seres Humanos
Imunoglobulinas Intravenosas/uso terapêutico
Fatores Imunológicos/uso terapêutico
Masculino
Meia-Idade
Condução Nervosa
Plasmaferese
Quadriplegia/etiologia
Reflexo Abdominal
Respiração Artificial
Insuficiência Respiratória/etiologia
Insuficiência Respiratória/terapia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunoglobulins, Intravenous); 0 (Immunologic Factors)
[Em] Mês de entrada:1404
[Cu] Atualização por classe:150811
[Lr] Data última revisão:
150811
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140131
[St] Status:MEDLINE


  7 / 100 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:23449670
[Au] Autor:Qu R; Tao J; Wang Y; Zhou Y; Wu G; Xiao Y; Hu CY; Jiang X; Xu GY
[Ad] Endereço:Institute of Neuroscience, Laboratory for Translational Pain Medicine, Department of Neurobiology, Soochow University, Suzhou, Peoples Republic of China.
[Ti] Título:Neonatal colonic inflammation sensitizes voltage-gated Na(+) channels via upregulation of cystathionine ß-synthetase expression in rat primary sensory neurons.
[So] Source:Am J Physiol Gastrointest Liver Physiol;304(9):G763-72, 2013 May 01.
[Is] ISSN:1522-1547
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The pathogenesis of pain in irritable bowel syndrome (IBS) is poorly understood, and treatment remains difficult. We have previously reported that colon-specific dorsal root ganglion (DRG) neurons were hyperactive in a rat model of IBS induced by neonatal colonic inflammation (NCI). This study was designed to examine plasticity of voltage-gated Na(+) channel activities and roles for the endogenous hydrogen sulfide-producing enzyme cystathionine ß-synthetase (CBS) in chronic visceral hyperalgesia. Abdominal withdrawal reflex (AWR) scores were recorded in response to graded colorectal distention in adult male rats as a measure of visceral hypersensitivity. Colon-specific DRG neurons were labeled with 1,1'-dioleyl-3,3,3',3-tetramethylindocarbocyanine methanesulfonate and acutely dissociated for measuring Na(+) channel currents. Western blot analysis was employed to detect changes in expressions of voltage-gated Na(+) (Na(V)) channel subtype 1.7, Na(V)1.8, and CBS. NCI significantly increased AWR scores when compared with age-matched controls. NCI also led to an ~2.5-fold increase in Na(+) current density in colon-specific DRG neurons. Furthermore, NCI dramatically enhanced expression of Na(V)1.7, Na(V)1.8, and CBS in colon-related DRGs. CBS was colocalized with Na(V)1.7 or -1.8 in colon-specific DRG neurons. Administration of O-(carboxymethyl)hydroxylamine hemihydrochloride (AOAA), an inhibitor for CBS, remarkably suppressed Na(+) current density and reduced expression of Na(V)1.7 and Na(V)1.8. More importantly, intraperitoneal or intrathecal application of AOAA attenuated AWR scores in NCI rats in a dose-dependent manner. These data suggest that NCI enhances Na(+) channel activity of colon DRG neurons, which is most likely mediated by upregulation of CBS expression, thus identifying a potential target for treatment for chronic visceral pain in patients with IBS.
[Mh] Termos MeSH primário: Colite/fisiopatologia
Cistationina beta-Sintase/biossíntese
Gânglios Espinais/fisiologia
Canal de Sódio Disparado por Voltagem NAV1.7/fisiologia
Canal de Sódio Disparado por Voltagem NAV1.8/fisiologia
[Mh] Termos MeSH secundário: Ácido Acético
Ácido Amino-Oxiacético/farmacologia
Animais
Animais Recém-Nascidos
Carbocianinas
Colite/induzido quimicamente
Corantes
Cistationina beta-Sintase/antagonistas & inibidores
Hiperalgesia/fisiopatologia
Síndrome do Intestino Irritável/fisiopatologia
Masculino
Canal de Sódio Disparado por Voltagem NAV1.7/biossíntese
Canal de Sódio Disparado por Voltagem NAV1.8/biossíntese
Ratos
Ratos Sprague-Dawley
Reflexo Abdominal/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (1,1'-dioleyl-3,3,3',3'-tetramethylindocarbocyanine); 0 (Carbocyanines); 0 (Coloring Agents); 0 (NAV1.7 Voltage-Gated Sodium Channel); 0 (NAV1.8 Voltage-Gated Sodium Channel); 0 (Scn10a protein, rat); 0 (Scn9a protein, rat); 14I68GI3OQ (Aminooxyacetic Acid); EC 4.2.1.22 (Cystathionine beta-Synthase); Q40Q9N063P (Acetic Acid)
[Em] Mês de entrada:1307
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130302
[St] Status:MEDLINE
[do] DOI:10.1152/ajpgi.00466.2012


  8 / 100 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:23139220
[Au] Autor:Hu S; Xiao Y; Zhu L; Li L; Hu CY; Jiang X; Xu GY
[Ad] Endereço:Institute of Neuroscience, Laboratory for Translational Pain Medicine, Department of Neurobiology, Soochow University, Suzhou, People's Republic of China.
[Ti] Título:Neonatal maternal deprivation sensitizes voltage-gated sodium channel currents in colon-specific dorsal root ganglion neurons in rats.
[So] Source:Am J Physiol Gastrointest Liver Physiol;304(4):G311-21, 2013 Feb 15.
[Is] ISSN:1522-1547
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Irritable bowel syndrome (IBS) is a common gastrointestinal disorder characterized by abdominal pain in association with altered bowel movements. The underlying mechanisms of visceral hypersensitivity remain elusive. This study was designed to examine the role for sodium channels in a rat model of chronic visceral hyperalgesia induced by neonatal maternal deprivation (NMD). Abdominal withdrawal reflex (AWR) scores were performed on adult male rats. Colon-specific dorsal root ganglion (DRG) neurons were labeled with DiI and acutely dissociated for measuring excitability and sodium channel current under whole-cell patch-clamp configurations. The expression of Na(V)1.8 was analyzed by Western blot and quantitative real-time PCR. NMD significantly increased AWR scores, which lasted for ~6 wk in an association with hyperexcitability of colon DRG neurons. TTX-resistant but not TTX-sensitive sodium current density was greatly enhanced in colon DRG neurons in NMD rats. Compared with controls, activation curves showed a leftward shift in NMD rats whereas inactivation curves did not differ significantly. NMD markedly accelerated the activation time of peak current amplitude without any changes in inactivation time. Furthermore, NMD remarkably enhanced expression of Na(V)1.8 at protein levels but not at mRNA levels in colon-related DRGs. The expression of Na(V)1.9 was not altered after NMD. These data suggest that NMD enhances TTX-resistant sodium activity of colon DRG neurons, which is most likely mediated by a leftward shift of activation curve and by enhanced expression of Na(V)1.8 at protein levels, thus identifying a specific molecular mechanism underlying chronic visceral pain and sensitization in patients with IBS.
[Mh] Termos MeSH primário: Colo/metabolismo
Gânglios Espinais/fisiologia
Privação Materna
Canal de Sódio Disparado por Voltagem NAV1.8/biossíntese
[Mh] Termos MeSH secundário: Animais
Hiperalgesia
Síndrome do Intestino Irritável
Masculino
Canal de Sódio Disparado por Voltagem NAV1.8/efeitos dos fármacos
Canal de Sódio Disparado por Voltagem NAV1.8/fisiologia
Neurônios/metabolismo
Ratos
Reflexo Abdominal
Tetrodotoxina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (NAV1.8 Voltage-Gated Sodium Channel); 0 (Scn10a protein, rat); 4368-28-9 (Tetrodotoxin)
[Em] Mês de entrada:1304
[Cu] Atualização por classe:130218
[Lr] Data última revisão:
130218
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121110
[St] Status:MEDLINE
[do] DOI:10.1152/ajpgi.00338.2012


  9 / 100 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:23285261
[Au] Autor:Wang Y; Qu R; Hu S; Xiao Y; Jiang X; Xu GY
[Ad] Endereço:Institute of Neuroscience, Key Laboratory of Pain Basic Research and Clinic Therapy, Department of Neurobiology, Soochow University, Suzhou, P. R. China.
[Ti] Título:Upregulation of cystathionine ß-synthetase expression contributes to visceral hyperalgesia induced by heterotypic intermittent stress in rats.
[So] Source:PLoS One;7(12):e53165, 2012.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Hydrogen sulfide (H2S) functions as a neuromodulator, but whether it modulates visceral pain is not well known. This study was designed to determine the role for the endogenous H2S producing enzyme cystathionine ß-synthetase (CBS) and cystathionine γ-lyase (CSE) in a validated rat model of visceral hyperalgesia (VH). METHODS: VH was induced by nine-day heterotypic intermittent stress (HIS). Abdominal withdrawal reflex (AWR) scores were determined by measuring the visceromoter responses to colorectal distension (CRD). Dorsal root ganglia (DRG) neurons innervating the colon were labeled by injection of DiI (1,1'-dioleyl-3,3,3',3-tetramethylindocarbocyanine methanesulfonate) into the colon wall. Patch clamp recording techniques were employed to examine excitability and sodium channel currents of colon specific DRG neurons. Tissues from colon related thoracolumbar DRGs were analyzed for CBS, CSE and sodium channel expression. RESULTS: HIS significantly increased the visceromotor responses to CRD in association with an upregulated expression of CBS not CSE proteins in colon related DRGs. Administration of O-(Carboxymethyl)hydroxylamine hemihydrochloride (AOAA), an inhibitor of CBS, attenuated the AWR scores in HIS-treated rats, in a dose dependent fashion. In contrast, AOAA did not produce any effect on AWR scores in healthy control rats. AOAA reversed the potentiation of sodium channel current densities of colon specific DRG neurons of HIS rats. To further confirm the role for CBS-H2S signaling, NaHS was used to mimic the production of H2S by CBS. Application of NaHS significantly enhanced neuronal excitability and potentiated sodium channel current densities of colon DRG neurons from healthy control rats. Furthermore, AOAA reversed the upregulation of Na(V)1.7 and Na(V)1.8 in colon related DRGs of HIS rats. CONCLUSION: Our results suggest that upregulation of CBS expression might play an important role in developing VH via sensitization of sodium channels in peripheral nociceptors, thus identifying a specific neurobiological target for the treatment of VH in functional bowel syndromes.
[Mh] Termos MeSH primário: Cistationina beta-Sintase/metabolismo
Hiperalgesia/enzimologia
Estresse Psicológico/fisiopatologia
Dor Visceral/enzimologia
[Mh] Termos MeSH secundário: Animais
Cistationina beta-Sintase/antagonistas & inibidores
Cistationina beta-Sintase/fisiologia
Inibidores Enzimáticos/farmacologia
Sulfeto de Hidrogênio/efeitos adversos
Sulfeto de Hidrogênio/metabolismo
Hiperalgesia/genética
Enteropatias/enzimologia
Enteropatias/etiologia
Enteropatias/genética
Masculino
Técnicas de Patch-Clamp
Ratos
Ratos Sprague-Dawley
Reflexo Abdominal/efeitos dos fármacos
Estresse Psicológico/genética
Sulfetos/farmacologia
Tacrolimo/análogos & derivados
Tacrolimo/farmacologia
Regulação para Cima/fisiologia
Vísceras/metabolismo
Vísceras/patologia
Dor Visceral/etiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (32-ascomycinyloxyacetic acid); 0 (Enzyme Inhibitors); 0 (Sulfides); EC 4.2.1.22 (Cystathionine beta-Synthase); FWU2KQ177W (sodium bisulfide); WM0HAQ4WNM (Tacrolimus); YY9FVM7NSN (Hydrogen Sulfide)
[Em] Mês de entrada:1307
[Cu] Atualização por classe:150219
[Lr] Data última revisão:
150219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130104
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0053165


  10 / 100 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:22930524
[Au] Autor:Li Q; Zhang X; Liu K; Gong L; Li J; Yao W; Liu C; Yu S; Li Y; Yao Z; Ma X
[Ad] Endereço:Department of Physiology, School of Medicine, Shandong University, Jinan, People's Republic of China.
[Ti] Título:Brain-derived neurotrophic factor exerts antinociceptive effects by reducing excitability of colon-projecting dorsal root ganglion neurons in the colorectal distention-evoked visceral pain model.
[So] Source:J Neurosci Res;90(12):2328-34, 2012 Dec.
[Is] ISSN:1097-4547
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The mechanism underlying visceral pain is still largely unclear. Recently, much attention has focused on a potential modulatory role of brain-derived neurotrophic factor (BDNF) in visceral pain. In the present study, we investigated the expression of BDNF in dorsal root ganglia (DRG) primary sensory neurons and its role in a colorectal distention (CRD)-induced model of visceral pain. Results obtained from enzyme-linked immunosorbant assay (ELISA) revealed that BDNF protein was upregulated after CRD. An abdominal withdrawal reflex (AWR) assay confirmed that BDNF played an antinociceptive role in this model. Application of BDNF directly to DRG neurons decreased their hypersensitivity when evoked by CRD. Pretreatment with k252a partially blocked the effect of BDNF. These findings suggest that BDNF might be a novel analgesic agent for the treatment of visceral pain.
[Mh] Termos MeSH primário: Fator Neurotrófico Derivado do Encéfalo/fisiologia
Gânglios Espinais/fisiopatologia
Hiperalgesia/fisiopatologia
Células Receptoras Sensoriais/fisiologia
Dor Visceral/fisiopatologia
[Mh] Termos MeSH secundário: Animais
Fator Neurotrófico Derivado do Encéfalo/antagonistas & inibidores
Fator Neurotrófico Derivado do Encéfalo/biossíntese
Fator Neurotrófico Derivado do Encéfalo/genética
Carbazóis/farmacologia
Células Cultivadas
Dilatação Patológica/complicações
Dilatação Patológica/fisiopatologia
Regulação da Expressão Gênica
Alcaloides de Indol/farmacologia
Masculino
Nociceptores/fisiologia
Medição da Dor
Técnicas de Patch-Clamp
Distribuição Aleatória
Ratos
Ratos Sprague-Dawley
Reflexo Abdominal/fisiologia
Método Simples-Cego
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Brain-Derived Neurotrophic Factor); 0 (Carbazoles); 0 (Indole Alkaloids); 97161-97-2 (staurosporine aglycone)
[Em] Mês de entrada:1304
[Cu] Atualização por classe:121019
[Lr] Data última revisão:
121019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120830
[St] Status:MEDLINE
[do] DOI:10.1002/jnr.23119



página 1 de 10 ir para página                        
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde