[PMID]: | 28854223 |
[Au] Autor: | Yu Y; Song EM; Lee KE; Joo YH; Kim SE; Moon CM; Kim HY; Jung SA; Jo I |
[Ad] Endereço: | Department of Molecular Medicine, College of Medicine, Ewha Womans University, Seoul, South Korea. |
[Ti] Título: | Therapeutic potential of tonsil-derived mesenchymal stem cells in dextran sulfate sodium-induced experimental murine colitis. |
[So] Source: | PLoS One;12(8):e0183141, 2017. |
[Is] ISSN: | 1932-6203 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | The therapeutic potential of tonsil-derived mesenchymal stem cells (TMSC) prepared from human tonsillar tissue has been studied in animal models for several diseases such as hepatic injury, hypoparathyroidism, diabetes and muscle dystrophy. In this study, we examined the therapeutic effects of TMSC in a dextran sulfate sodium (DSS)-induced colitis model. TMSC were injected in DSS-induced colitis mice via intraperitoneal injection twice (TMSC[x2]) or four times (TMSC[x4]). Control mice were injected with either phosphate-buffered saline or human embryonic kidney 293 cells. Body weight, stool condition and disease activity index (DAI) were examined daily. Colon length, histologic grading, and mRNA expression of pro-inflammatory cytokines, interleukin 1ß (IL-1ß), IL-6, IL-17 and tumor necrosis factor α, and anti-inflammatory cytokines, IL-10, IL-11 and IL-13, were also measured. Our results showed a significant improvement in survival rates and body weight gain in colitis mice injected with TMSC[x2] or TMSC[x4]. Injection with TMSC also significantly decreased DAI scores throughout the experimental period; at the end of experiment, almost complete reversal of DAI scores to normal was found in colitis mice treated with TMSC[x4]. Colon length was also significantly recovered in colitis mice treated with TMSC[x4]. However, histopathological alterations induced by DSS treatment were not apparently improved by injection with TMSC. Finally, treatment with TMSC[x4] significantly reversed the mRNA levels of IL-1ß and IL-6, although expression of all pro-inflammatory cytokines tested was induced in colitis mice. Under our experimental conditions, however, no apparent alterations in the mRNA levels of all the anti-inflammatory cytokines tested were found. In conclusion, our findings demonstrate that multiple injections with TMSC produced a therapeutic effect in a mouse model of DSS-induced colitis. |
[Mh] Termos MeSH primário: |
Terapia Baseada em Transplante de Células e Tecidos/métodos Colite/terapia Transplante de Células-Tronco Mesenquimais Células Mesenquimais Estromais/citologia Tonsila Palatina/citologia
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[Mh] Termos MeSH secundário: |
Animais Peso Corporal Criança Colite/induzido quimicamente Colite/imunologia Colite/patologia Sulfato de Dextrana Modelos Animais de Doenças Regulação da Expressão Gênica Células HEK293 Seres Humanos Injeções Intraperitoneais Interleucina-10/genética Interleucina-10/imunologia Interleucina-11/genética Interleucina-11/imunologia Interleucina-13/genética Interleucina-13/imunologia Interleucina-17/genética Interleucina-17/imunologia Interleucina-1beta/genética Interleucina-1beta/imunologia Interleucina-6/genética Interleucina-6/imunologia Masculino Células Mesenquimais Estromais/fisiologia Camundongos Camundongos Endogâmicos C57BL Tonsila Palatina/fisiologia Tonsila Palatina/cirurgia Recuperação de Função Fisiológica/fisiologia Análise de Sobrevida Resultado do Tratamento Fator de Necrose Tumoral alfa/genética Fator de Necrose Tumoral alfa/imunologia
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (IL10 protein, mouse); 0 (IL1B protein, mouse); 0 (Interleukin-11); 0 (Interleukin-13); 0 (Interleukin-17); 0 (Interleukin-1beta); 0 (Interleukin-6); 0 (Tumor Necrosis Factor-alpha); 0 (interleukin-6, mouse); 130068-27-8 (Interleukin-10); 9042-14-2 (Dextran Sulfate) |
[Em] Mês de entrada: | 1710 |
[Cu] Atualização por classe: | 171016 |
[Lr] Data última revisão:
| 171016 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170831 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1371/journal.pone.0183141 |
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