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[PMID]:29221731
[Au] Autor:Han SJ; Glatman Zaretsky A; Andrade-Oliveira V; Collins N; Dzutsev A; Shaik J; Morais da Fonseca D; Harrison OJ; Tamoutounour S; Byrd AL; Smelkinson M; Bouladoux N; Bliska JB; Brenchley JM; Brodsky IE; Belkaid Y
[Ad] Endereço:Mucosal Immunology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, MD 20892, USA.
[Ti] Título:White Adipose Tissue Is a Reservoir for Memory T Cells and Promotes Protective Memory Responses to Infection.
[So] Source:Immunity;47(6):1154-1168.e6, 2017 Dec 19.
[Is] ISSN:1097-4180
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:White adipose tissue bridges body organs and plays a fundamental role in host metabolism. To what extent adipose tissue also contributes to immune surveillance and long-term protective defense remains largely unknown. Here, we have shown that at steady state, white adipose tissue contained abundant memory lymphocyte populations. After infection, white adipose tissue accumulated large numbers of pathogen-specific memory T cells, including tissue-resident cells. Memory T cells in white adipose tissue expressed a distinct metabolic profile, and white adipose tissue from previously infected mice was sufficient to protect uninfected mice from lethal pathogen challenge. Induction of recall responses within white adipose tissue was associated with the collapse of lipid metabolism in favor of antimicrobial responses. Our results suggest that white adipose tissue represents a memory T cell reservoir that provides potent and rapid effector memory responses, positioning this compartment as a potential major contributor to immunological memory.
[Mh] Termos MeSH primário: Tecido Adiposo Branco/transplante
Linfócitos T CD4-Positivos/imunologia
Linfócitos T CD8-Positivos/imunologia
Memória Imunológica
Toxoplasmose/imunologia
Infecções por Yersinia pseudotuberculosis/imunologia
[Mh] Termos MeSH secundário: Tecido Adiposo Branco/imunologia
Animais
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Linfócitos T CD4-Positivos/microbiologia
Linfócitos T CD4-Positivos/parasitologia
Linfócitos T CD8-Positivos/microbiologia
Linfócitos T CD8-Positivos/parasitologia
Expressão Gênica
Genes Reporter
Interferon gama/genética
Interferon gama/imunologia
Interleucina-17/genética
Interleucina-17/imunologia
Interleucina-5/genética
Interleucina-5/imunologia
Metabolismo dos Lipídeos
Proteínas Luminescentes/genética
Proteínas Luminescentes/metabolismo
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Transgênicos
Análise de Sobrevida
Transplante de Tecidos
Toxoplasma/imunologia
Toxoplasmose/genética
Toxoplasmose/mortalidade
Toxoplasmose/parasitologia
Fator de Necrose Tumoral alfa/genética
Fator de Necrose Tumoral alfa/imunologia
Yersinia pseudotuberculosis/imunologia
Infecções por Yersinia pseudotuberculosis/genética
Infecções por Yersinia pseudotuberculosis/microbiologia
Infecções por Yersinia pseudotuberculosis/mortalidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Interleukin-17); 0 (Interleukin-5); 0 (Luminescent Proteins); 0 (Tumor Necrosis Factor-alpha); 0 (yellow fluorescent protein, Bacteria); 82115-62-6 (Interferon-gamma)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180210
[Lr] Data última revisão:
180210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171210
[St] Status:MEDLINE


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[PMID]:29310397
[Au] Autor:Ma XN; Qiang S; Liu TY; Cao MY; Lv SC
[Ad] Endereço:Department of Orthopedics.
[Ti] Título:Massive rare desmoplastic fibroma of the ilium and ischium in a young adult: A case report.
[So] Source:Medicine (Baltimore);96(48):e8962, 2017 Dec.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RATIONALE: Desmoplastic fibroma (DF) is a rare, locally invasive but benign bone tumor. It represents one of the rarest bone diseases, with an incidence of only 0.11% of all primary bone tumors. PATIENT CONCERNS: Herein, a case of massive and unusual DF, with simultaneous involvement of ilium and ischium, is described. A 29-year-old man suffered minor pain in his right hip for 2 years. It worsened after sudden movements, which prevented him from walking normally. Physical examination showed a limitation when the right hip was flexed and a percussion pain on the hip region. A medical imaging examination showed that the right ilium and ischium had a massive bone lesion. The top of acetabular had very little bone left and a fracture was likely at any time. No prominent body weight loss was noted, because there was no extensive invasion to the adjacent soft tissue. DIAGNOSES: DF of the Ilium and Ischium. INTERVENTIONS: The patient underwent a surgery involving curettage and grafting to maintain the stability of the pelvis. OUTCOMES: The definitive pathological diagnosis was DF, without evidence of malignancy. The postoperative recovery course at 3-month follow-up was uneventful. LESSONS: To the authors' knowledge, such a massive DF involving both ilium and ischium has been rarely reported. Young patients require appropriate and timely treatment modalities.
[Mh] Termos MeSH primário: Neoplasias Ósseas/cirurgia
Fibroma Desmoplásico/cirurgia
Ílio/cirurgia
Ísquio/cirurgia
[Mh] Termos MeSH secundário: Adulto
Neoplasias Ósseas/diagnóstico por imagem
Neoplasias Ósseas/patologia
Curetagem
Fibroma Desmoplásico/diagnóstico por imagem
Fibroma Desmoplásico/patologia
Seres Humanos
Ílio/diagnóstico por imagem
Ílio/patologia
Ísquio/diagnóstico por imagem
Ísquio/patologia
Masculino
Transplante de Tecidos
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180110
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000008962


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[PMID]:28817617
[Au] Autor:Hongyong J; Shuzhu C; Min W; Weijing Y; Yidong L
[Ad] Endereço:The Urology Center of XinJiang Uygur Autonomous Region People's Hospital, Urumqi, China.
[Ti] Título:Comparison of lingual mucosa and buccal mucosa grafts used in inlay urethroplasty in failed hypospadias of pre-pubertal boys in a Chinese group.
[So] Source:PLoS One;12(8):e0182803, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: The purpose of this study was to compare the outcomes of the buccal mucosa and lingual mucosa used in children who received multiple failed hypospadias surgeries. METHOD: We conducted a retrospective study of 62 children who received buccal or lingual mucosa graft urethroplasty in our hospital between 2012 and 2015. The ages ranged from 3.5-11 y. All cases included multiple failed hypospadias procedures, and the subjects received previous operations 2-3 times. All patients underwent one-stage operations. Thirty-three cases were treated with lingual mucosa grafts. The patient ages ranged from 3.5 to 11 y (median 7.5 y), and they had previous operations 2-3 times (mean 2.8±0.7). Grafts ranged from lengths of 2-6 cm (mean 5.1±0.46 cm) and widths of 0.5-1.5 cm (mean 1.2± 0.16 cm). Our follow-up was 5 to 12 m (mean 8.3±1.2 m). Twenty-nine cases were treated with buccal mucosa grafts. The patient ages ranged from 4 to 9.2 y (median 7.0 y), and they had previous operations 2-3 times (mean 2.5±0.2). Grafts ranged from lengths of 2-5.3 cm (mean 4.9± 0.28 cm) and widths of 0.5-1.5 cm (mean 1.0±0.11 cm). Our follow-up was 5 to 12 m (mean 7.9±0.5 m). The results were tested with SPSS 18.0. The rates of complications were compared by a chi-square test, and pre-operative conditions were compared by t test. RESULTS: For the outcomes of the two groups, there was no significant difference between the groups in terms of age, preoperative surgery time, and the length and width of the grafts (p>0.05). For the lingual mucosa graft group, fistula: 2/33 (6.0%), stricture: 1/33(3.0%), ventral curvature: 2/33(6.0%), complications: 5/33(15.0%), success rate: 28/33(84.8%), Hose score: 14.34±0.95, peak flow: 6.5 ml/s-12.0 ml/s, and mean peak flow: 9.3±0.4 ml/s. For the buccal mucosa graft group, fistula: 2/29(6.8%), stricture: 2/29(6.8%), ventral curvature: 1/29 (3.4%), complication rate: 5/29(17.0%), success rate: 24/29 (83.0%), Hose score: 14.28±1.03, peak flow: 6.5 ml/s-12.0 ml/s, and mean peak flow: 9.2±0.2 ml/s. There were no differences between the two groups for overall success, complication rates, peak flow, and the Hose scores(P>0.05). CONCLUSION: The lingual mucosal graft and the buccal mucosa graft both achieved good outcomes, and the lingual mucosa graft made up for the shortcomings of the buccal mucosa graft, which provided a reliable way to treat the multiple failed hypospadias surgeries in pre-pubertal boys.
[Mh] Termos MeSH primário: Autoenxertos/transplante
Hipospadia/cirurgia
Mucosa Bucal/transplante
Transplante de Tecidos/métodos
Procedimentos Cirúrgicos Urológicos Masculinos/métodos
[Mh] Termos MeSH secundário: Criança
Pré-Escolar
Seres Humanos
Masculino
Complicações Pós-Operatórias
Transplante de Tecidos/efeitos adversos
Língua/transplante
Uretra/cirurgia
Procedimentos Cirúrgicos Urológicos Masculinos/efeitos adversos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170818
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182803


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[PMID]:28715799
[Au] Autor:He Y; Xu X; Zhu T; Tang M; Mei J; Si Y
[Ad] Endereço:Department of Cardiovascular Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.
[Ti] Título:Resident Arterial Cells and Circulating Bone Marrow-Derived Cells both Contribute to Intimal Hyperplasia in a Rat Allograft Carotid Transplantation Model.
[So] Source:Cell Physiol Biochem;42(4):1303-1312, 2017.
[Is] ISSN:1421-9778
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIMS: Neointimal formation following vascular injury remains a major mechanism of restenosis, whereas the precise sources of neointimal cells are still uncertain. We tested the hypothesis that both injured arterial cells and non-arterial cells contribute to intimal hyperplasia. METHODS: Following allograft transplantation of the balloon-injured carotid common artery (n = 3-6), the cellular composition of the transplant grafts and the origins of neointimal cells were measured by immunohistochemistry and immunofluorescence staining. RESULTS: Smooth muscle actin (SMA)-positive and CD68-positive cells were clearly observed 14 days later in the neointima after allograft transplantation of the balloon-injured carotid common artery, where re-endothelialization was not yet complete. Green fluorescent protein (GFP) and wild-type (WT) allograft transplantation revealed that the majority of the neointima cells were apparently from the recipient (≈85%) versus the donor (≈15%). Both monocyte chemotactic protein-1 (MCP-1)/CCR2 and stromal cell-derived factor-1 (SDF-1)/CXCR4 signaling were involved in intimal hyperplasia, with bone marrow-derived cells also playing a role. CONCLUSION: These data support the hypothesis that intimal hyperplasia could develop in our novel rat allograft transplantation model of arterial injury, where neointima is attributable not only to local arterial cells but also non-arterial cells including the bone marrow.
[Mh] Termos MeSH primário: Células da Medula Óssea/patologia
Lesões das Artérias Carótidas/patologia
Artéria Carótida Primitiva/patologia
Células Endoteliais/patologia
Neointima/patologia
Transplante de Tecidos
Túnica Íntima/lesões
[Mh] Termos MeSH secundário: Actinas/genética
Actinas/imunologia
Animais
Antígenos CD/genética
Antígenos CD/imunologia
Antígenos de Diferenciação Mielomonocítica/genética
Antígenos de Diferenciação Mielomonocítica/imunologia
Células da Medula Óssea/imunologia
Lesões das Artérias Carótidas/genética
Lesões das Artérias Carótidas/imunologia
Lesões das Artérias Carótidas/cirurgia
Artéria Carótida Primitiva/imunologia
Artéria Carótida Primitiva/cirurgia
Linhagem da Célula/imunologia
Rastreamento de Células
Quimiocina CCL2/genética
Quimiocina CCL2/imunologia
Quimiocina CXCL12/genética
Quimiocina CXCL12/imunologia
Células Endoteliais/imunologia
Regulação da Expressão Gênica
Genes Reporter
Proteínas de Fluorescência Verde/genética
Proteínas de Fluorescência Verde/imunologia
Neointima/genética
Neointima/imunologia
Neointima/cirurgia
Ratos
Ratos Endogâmicos Lew
Ratos Transgênicos
Receptores CCR2/genética
Receptores CCR2/imunologia
Receptores CXCR4/genética
Receptores CXCR4/imunologia
Transdução de Sinais
Transplante Homólogo
Túnica Íntima/imunologia
Túnica Íntima/cirurgia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actins); 0 (Antigens, CD); 0 (Antigens, Differentiation, Myelomonocytic); 0 (CD68 protein, rat); 0 (CXCL12 protein, rat); 0 (Ccl2 protein, rat); 0 (Ccr2 protein, rat); 0 (Chemokine CCL2); 0 (Chemokine CXCL12); 0 (Cxcr4 protein, rat); 0 (Receptors, CCR2); 0 (Receptors, CXCR4); 0 (smooth muscle actin, rat); 147336-22-9 (Green Fluorescent Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171026
[Lr] Data última revisão:
171026
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170718
[St] Status:MEDLINE
[do] DOI:10.1159/000478959


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[PMID]:28610970
[Au] Autor:Wang L; Lu S; Qian H; Shi X
[Ad] Endereço:Department of Neurosurgery, Fu Xing Hospital, Capital Medical University, Beijing, China; Department of Neurosurgery, SanBo Brain Hospital, Capital Medical University, Beijing, China.
[Ti] Título:Internal Maxillary Artery Bypass with Radial Artery Graft Treatment of Giant Intracranial Aneurysms.
[So] Source:World Neurosurg;105:568-584, 2017 Sep.
[Is] ISSN:1878-8769
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Giant intracranial aneurysms (GIAs) can be approached via microsurgical and endovascular techniques. Use of flow diversion devices has yielded favorable outcomes, and indications for cerebral revascularization have dramatically decreased. OBJECTIVE: To evaluate the safety and validity of using internal maxillary artery (IMA) bypass with an interposed graft to treat GIAs. METHODS: During a 6-year period, high-flow IMA bypass procedures were performed in 32 patients with GIAs at our institute. Intraoperative Doppler sonography, indocyanine green, and postoperative angiography were used to assess the patency of the graft conduit and the stability of the aneurysm. Neurologic function was assessed with the modified Rankin Scale. RESULTS: The mean GIA size was 33.7 mm (range, 25.3-64 mm), and all showed a nonsaccular morphology, except for 1 patient who underwent a first attempt at surgical clipping of a saccular aneurysm. Complete occlusion (65.6%, n = 21) or great diminishment (28.1%, n = 9) of the GIA was shown in 30 patients and the graft patency rate was 90.6% (n = 29). No deaths resulting from bypass surgery occurred. Functionally favorable outcomes (modified Rankin Scale score of 0-2) were recorded in 65.6% of patients at discharge, and the rate increased to 71.9% at the most recent follow-up (mean, 40.6 months). Surgical-related complications involving hydrocephalus and ischemic and hemorrhagic episodes were encountered in 5 patients, but 4 had complete regression of their neurologic symptoms. CONCLUSIONS: Considering the grave natural history and treatment complexity of these lesions, this study showed satisfactory results in the treatment of GIAs with IMA bypass.
[Mh] Termos MeSH primário: Revascularização Cerebral/métodos
Aneurisma Intracraniano/cirurgia
Artéria Maxilar/cirurgia
Artéria Radial/transplante
[Mh] Termos MeSH secundário: Adolescente
Adulto
Angiografia Cerebral/métodos
Feminino
Seguimentos
Seres Humanos
Aneurisma Intracraniano/diagnóstico por imagem
Masculino
Artéria Maxilar/diagnóstico por imagem
Meia-Idade
Estudos Prospectivos
Artéria Radial/diagnóstico por imagem
Estudos Retrospectivos
Transplante de Tecidos/métodos
Resultado do Tratamento
Ultrassonografia Doppler/métodos
Adulto Jovem
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170615
[St] Status:MEDLINE


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[PMID]:28577786
[Au] Autor:Chao J; Pao A
[Ad] Endereço:Peachtree Orthopaedic Clinic, 5505 Peachtree Dunwoody Road, Suite 600, Atlanta, GA 30342, USA. Electronic address: jchao@pocatlanta.com.
[Ti] Título:Restorative Tissue Transplantation Options for Osteochondral Lesions of the Talus: A Review.
[So] Source:Orthop Clin North Am;48(3):371-383, 2017 Jul.
[Is] ISSN:1558-1373
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Symptomatic osteochondral lesions of the talus remain a challenging problem due to inability for cartilage lesions to heal. Numerous treatment options exist, including nonoperative management, marrow stimulating techniques, and autograft-allograft. Arthroscopic marrow stimulation forms fibrocartilage that has been shown to be biomechanically weaker than hyaline cartilage. Restorative tissue transplantation options are being used more for larger and cystic lesions. Newer biologics and particulated juvenile cartilage are currently under investigation for possible clinical efficacy. This article provides an evidenced-based summary of available literature on the use of biologics for treatment of osteochondral lesions of the talus.
[Mh] Termos MeSH primário: Terapia Biológica/métodos
Cartilagem Articular
Osteocondroma
Tálus
Transplante de Tecidos/métodos
[Mh] Termos MeSH secundário: Cartilagem Articular/patologia
Cartilagem Articular/cirurgia
Seres Humanos
Osteocondroma/patologia
Osteocondroma/fisiopatologia
Osteocondroma/cirurgia
Tálus/patologia
Tálus/fisiopatologia
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170605
[St] Status:MEDLINE


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[PMID]:28574951
[Au] Autor:Duisit J; Amiel H; Debluts D; Maistriaux L; Gerdom A; Bol A; Gianello P; Behets C; Lengelé B
[Ad] Endereço:Brussels, Belgium From the Department of Plastic and Reconstructive Surgery, Cliniques Universitaires Saint-Luc, and Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain.
[Ti] Título:Single-Artery Human Ear Graft Procurement: A Simplified Approach.
[So] Source:Plast Reconstr Surg;140(3):599-603, 2017 Sep.
[Is] ISSN:1529-4242
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In the field of experimental facial vascularized composite tissue allotransplantation, a human auricular subunit model, pedicled on both superficial temporal and posterior auricular arteries, was described. Clinical cases of extensive auricular replantation, however, suggested that a single artery could perfuse the entire flap. In this study, variants of this single-pedicle approach have been studied, aiming to develop a more versatile replantation technique, in which the question of venous drainage has also been addressed. For arterial perfusion study, the authors harvested 11 auricular grafts, either on a single superficial temporal artery pedicle (n = 3) or a double superficial temporal and posterior auricular artery pedicle (n = 8). The authors then proceeded to selective barium injections, in the superficial temporal, posterior auricular, or both superficial temporal and posterior auricular arteries. Arteriograms were acquired with a micro-computed tomographic scan and analyzed on three-dimensionally reconstructed images. Venous drainage was investigated in eight hemifaces, carefully dissected after latex injection. Observations showed a homogenous perfusion of the whole auricle in all arterial graft variants. Venous drainage was highly variable, with either a dominant superficial temporal vein (37.5 percent), dominant posterior auricular vein (12.5 percent), or co-dominant trunks (50 percent). The authors demonstrated that auricular subunit vascularized composite tissue allotransplantation can be performed on a single artery, relying on the dynamic intraauricular anastomoses between superficial temporal artery and posterior auricular branches. Potentially, this vascular versatility is prone to simplify the subunit harvest and allows various strategies for pedicle selection. Venous drainage, however, remains inconstant and thus the major issue when considering auricular transplantation. CLINICAL QUESTION/LEVEL OF EVIDENCE: Therapeutic, V.
[Mh] Termos MeSH primário: Artérias/anatomia & histologia
Pavilhão Auricular/irrigação sanguínea
Retalhos Cirúrgicos/irrigação sanguínea
Obtenção de Tecidos e Órgãos/métodos
[Mh] Termos MeSH secundário: Artérias/diagnóstico por imagem
Seres Humanos
Modelos Biológicos
Procedimentos Cirúrgicos Reconstrutivos/métodos
Transplante de Tecidos/métodos
Veias/anatomia & histologia
Veias/diagnóstico por imagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170603
[St] Status:MEDLINE
[do] DOI:10.1097/PRS.0000000000003591


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[PMID]:28574947
[Au] Autor:Luan A; Zielins ER; Wearda T; Atashroo DA; Blackshear CP; Raphel J; Brett EA; Flacco J; Alyono MC; Momeni A; Heilshorn S; Longaker MT; Wan DC
[Ad] Endereço:Stanford, Calif. From the Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, and the Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine; and the Department of Materials Science and Engineering, Stanford University.
[Ti] Título:Dynamic Rheology for the Prediction of Surgical Outcomes in Autologous Fat Grafting.
[So] Source:Plast Reconstr Surg;140(3):517-524, 2017 Sep.
[Is] ISSN:1529-4242
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Because of the abundance and biocompatibility of fat, lipotransfer has become an attractive method for treating soft-tissue deficits. However, it is limited by unpredictable graft survival and retention. Currently, little is known about the viscoelastic properties of fat after various injection methods. Here, the authors assess the effects of cannula diameter, length, and shape on the viscoelastic properties, structure, and retention of fat. METHODS: Human lipoaspirate was harvested using suction-assisted liposuction and prepared for grafting. A syringe pump was used to inject fat at a controlled flow rate through cannulas of varying gauges, lengths, and shapes. Processed samples were tested in triplicate on an oscillatory rheometer to measure their viscoelastic properties. Fat grafts from each group were placed into the scalps of immunocompromised mice. After 8 weeks, graft retention was measured using micro-computed tomography and grafts were explanted for histologic analysis. RESULTS: Lipoaspirate injected through narrower, longer, and bent cannulas exhibited more shear thinning with diminished quality. The storage modulus (G') of fat processed with 18-gauge cannulas was significantly lower than when processed with 14-gauge or larger cannulas, which also corresponded with inferior in vivo histologic structure. Similarly, the longer cannula group had a significantly lower storage modulus than the shorter cannula, and was associated with decreased graft retention. CONCLUSIONS: Discrete modifications in the methods used for fat placement can have a significant impact on immediate graft integrity, and ultimately on graft survival and quality. Respecting these biomechanical influences during the placement phase of lipotransfer may allow surgeons to optimize outcomes. CLINICAL QUESTION/LEVEL OF EVIDENCE: Therapeutic, V.
[Mh] Termos MeSH primário: Tecido Adiposo/fisiologia
Tecido Adiposo/transplante
Cateteres
Sobrevivência de Enxerto/fisiologia
Transplante de Tecidos/métodos
[Mh] Termos MeSH secundário: Adipócitos/transplante
Animais
Modelos Animais de Doenças
Desenho de Equipamento
Seres Humanos
Camundongos
Transplante Autólogo
Microtomografia por Raio-X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170603
[St] Status:MEDLINE
[do] DOI:10.1097/PRS.0000000000003578


  9 / 2395 MEDLINE  
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[PMID]:28507225
[Au] Autor:Lee P; Jiang S; Li Y; Yue J; Gou X; Chen SY; Zhao Y; Schober M; Tan M; Wu X
[Ad] Endereço:Ben May Department for Cancer Research, The University of Chicago, Chicago, IL, USA.
[Ti] Título:Phosphorylation of Pkp1 by RIPK4 regulates epidermal differentiation and skin tumorigenesis.
[So] Source:EMBO J;36(13):1963-1980, 2017 Jul 03.
[Is] ISSN:1460-2075
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Tissue homeostasis of skin is sustained by epidermal progenitor cells localized within the basal layer of the skin epithelium. Post-translational modification of the proteome, such as protein phosphorylation, plays a fundamental role in the regulation of stemness and differentiation of somatic stem cells. However, it remains unclear how phosphoproteomic changes occur and contribute to epidermal differentiation. In this study, we survey the epidermal cell differentiation in a systematic manner by combining quantitative phosphoproteomics with mammalian kinome cDNA library screen. This approach identified a key signaling event, phosphorylation of a desmosome component, PKP1 (plakophilin-1) by RIPK4 (receptor-interacting serine-threonine kinase 4) during epidermal differentiation. With genome-editing and mouse genetics approach, we show that loss of function of either or impairs skin differentiation and enhances epidermal carcinogenesis Phosphorylation of PKP1's N-terminal domain by RIPK4 is essential for their role in epidermal differentiation. Taken together, our study presents a global view of phosphoproteomic changes that occur during epidermal differentiation, and identifies RIPK-PKP1 signaling as novel axis involved in skin stratification and tumorigenesis.
[Mh] Termos MeSH primário: Diferenciação Celular
Queratinócitos/fisiologia
Placofilinas/metabolismo
Processamento de Proteína Pós-Traducional
Proteínas Serina-Treonina Quinases/metabolismo
Pele/citologia
Células-Tronco/fisiologia
[Mh] Termos MeSH secundário: Animais
Carcinogênese
Células Cultivadas
Perfilação da Expressão Gênica
Camundongos
Camundongos Knockout
Fosforilação
Proteoma/análise
Neoplasias Cutâneas
Transplante de Tecidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pkp1 protein, mouse); 0 (Plakophilins); 0 (Proteome); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); EC 2.7.11.1 (Ripk4 protein, mouse)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170517
[St] Status:MEDLINE
[do] DOI:10.15252/embj.201695679


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[PMID]:28254539
[Au] Autor:Mattavelli D; Schreiber A; Ferrari M; Accorona R; Bolzoni Villaret A; Battaglia P; Castelnuovo P; Nicolai P
[Ad] Endereço:Unit of Otorhinolaryngology-Head and Neck Surgery, Department of Surgical Specialties, Radiological Sciences, and Public Health, University of Brescia, Brescia, Italy. Electronic address: davide_mattavelli85@yahoo.it.
[Ti] Título:Three-Layer Reconstruction with Iliotibial Tract After Endoscopic Resection of Sinonasal Tumors.
[So] Source:World Neurosurg;101:486-492, 2017 May.
[Is] ISSN:1878-8769
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Watertight reconstruction to separate the intradural compartment from the sinonasal cavities is crucial after endoscopic resection with transnasal craniectomy for nasoethmoidal tumors. A 3-layer reconstruction with the iliotibial tract is a safe and reliable alternative when vascularized flaps are unavailable. The iliotibial tract graft is harvested on the lateral aspect of the thigh and divided into 3 portions, which are positioned in a multilayered fashion to close the skull base defect: the intracranial intradural layer (first layer), the intracranial extradural layer (second layer), and the extracranial extradural layer (third layer). Fat grafts from thigh subcutaneous tissue are placed between the second and third layers to fill the dead space between them. Use of fibrin glue and intradural irrigation may help the surgeon to stabilize the layers during reconstruction. Three-layer reconstruction with the iliotibial tract is a feasible, highly reproducible, safe, and always available option for reconstruction of anterior skull base defects after endoscopic resection with transnasal craniectomy for nasoethmoidal tumors.
[Mh] Termos MeSH primário: Neuroendoscopia/métodos
Procedimentos Cirúrgicos Reconstrutivos/métodos
Neoplasias da Base do Crânio/cirurgia
Transplante de Tecidos/métodos
Coleta de Tecidos e Órgãos/métodos
[Mh] Termos MeSH secundário: Craniectomia Descompressiva/métodos
Seres Humanos
Ligamentos Articulares/cirurgia
Ligamentos Articulares/transplante
Cavidade Nasal/cirurgia
Neoplasias da Base do Crânio/diagnóstico
Coxa da Perna/cirurgia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170304
[St] Status:MEDLINE



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