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[PMID]:29340525
[Au] Autor:França CN; Mendes CC; Ferreira CES
[Ad] Endereço:Pós Graduação em Ciências da Saúde, Universidade Santo Amaro, São Paulo, SP, Brasil.
[Ti] Título:Time collection and storage conditions of lipid profile.
[So] Source:Braz J Med Biol Res;51(3):e6955, 2018 Jan 11.
[Is] ISSN:1414-431X
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The stability of samples is crucial for getting reliable concentrations of many analytes, including lipid profile. Thus, the goal of this study was to analyze lipid profile under different storage and temperature conditions. This was a prospective study with 809 patients of both genders. Total cholesterol, triglycerides, high-density lipoprotein cholesterol, low density lipoprotein cholesterol and non-high-density lipoprotein were measured within 1 h from collection at room temperature, after 2-3 h of refrigeration (8°C) and after 4-5 h at room temperature. The processing time and storage conditions did not affect the analytes measured. These findings are important for multicenter studies, because of the difficulties related to centrifugation and freezing of samples immediately after collection.
[Mh] Termos MeSH primário: Coleta de Amostras Sanguíneas/métodos
Lipídeos/sangue
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Análise Química do Sangue
Preservação de Sangue
Coleta de Amostras Sanguíneas/instrumentação
Coleta de Amostras Sanguíneas/normas
Colesterol/sangue
Feminino
Seres Humanos
Laboratórios/normas
Lipoproteínas/sangue
Masculino
Meia-Idade
Estudos Prospectivos
Temperatura Ambiente
Fatores de Tempo
Triglicerídeos/sangue
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lipids); 0 (Lipoproteins); 0 (Triglycerides); 97C5T2UQ7J (Cholesterol)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE


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[PMID]:27771280
[Au] Autor:Chappidi MR; Chalfin HJ; Johnson DJ; Kates M; Sopko NA; Johnson MH; Liu JJ; Frank SM; Bivalacqua TJ
[Ad] Endereço:The James Buchanan Brady Urological Institute, The Johns Hopkins University School of Medicine, Baltimore, MD. Electronic address: mchappi1@jhmi.edu.
[Ti] Título:Longer average blood storage duration is associated with increased risk of infection and overall morbidity following radical cystectomy.
[So] Source:Urol Oncol;35(2):38.e17-38.e24, 2017 02.
[Is] ISSN:1873-2496
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Patients with bladder cancer undergoing radical cystectomy (RC) experience high rates of perioperative blood transfusions (PBTs) and morbidity. The aim of this study was to evaluate the effect of blood storage duration on the risk of adverse perioperative outcomes in this high-risk patient population. MATERIALS AND METHODS: In a retrospective review of RC patients from 2010 to 2014 who received PBTs, the average storage duration for all units transfused was used to classify patients as receiving older blood using 3 different definitions (≥21 days,≥28 days, and≥35 days). Multivariable Poisson regression models were used to determine the adjusted relative risk of perioperative infections and overall morbidity in those given older blood compared to fresher blood. RESULTS: Of the 451 patients undergoing RC, 205 (45%) received nonirradiated PBTs. In multivariable modeling, increasing average blood storage duration, as a continuous variable, was associated with an increased risk of infections (risk ratio [RR] = 1.08 per day, 95% CI: 1.01-1.17) and overall morbidity (RR = 1.08 per day, 95% CI: 1.01-1.15). Furthermore, ≥28-day blood storage (vs.<28) was associated with increased infections (RR = 2.69, 95% CI: 1.18-6.14) and morbidity (RR = 2.54, 95% CI: 1.31-4.95), and ≥35-day blood storage (vs.<35) was also associated with increased infections (RR = 2.83, 95% CI: 1.42-5.66) and morbidity (RR = 3.35, 95% CI: 1.95-5.77). CONCLUSIONS: Although blood is stored up to 42 days, storage≥28 days may expose RC patients to increased perioperative infections and overall morbidity compared with storage<28 days. Prospective cohort studies are warranted in cystectomy and other high-risk surgical oncology patients to better determine the effect of blood storage duration.
[Mh] Termos MeSH primário: Preservação de Sangue/métodos
Cistectomia/métodos
Infecção/diagnóstico
Neoplasias da Bexiga Urinária/cirurgia
[Mh] Termos MeSH secundário: Idoso
Preservação de Sangue/efeitos adversos
Transfusão de Sangue/métodos
Comorbidade
Feminino
Seres Humanos
Infecção/epidemiologia
Infecção/etiologia
Masculino
Meia-Idade
Avaliação de Resultados (Cuidados de Saúde)/métodos
Avaliação de Resultados (Cuidados de Saúde)/estatística & dados numéricos
Estudos Retrospectivos
Fatores de Risco
Fatores de Tempo
Neoplasias da Bexiga Urinária/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:29198036
[Au] Autor:Hara J; Tanaka Y; Kaneko H; Itoh Y; Ikegaya H
[Ad] Endereço:Department of Forensic Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajiicho, Kamigyo, Kyoto, 602-8566, Japan.
[Ti] Título:Detection of hepatitis B virus DNA and HBsAg from postmortem blood and bloodstains.
[So] Source:Arch Virol;163(3):633-637, 2018 Mar.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:A large number of accidental virus infections occur in medical and non-medical workers exposed to infectious individuals and materials. We evaluated whether postmortem blood and bloodstains containing hepatitis B virus (HBV) are infectious. HBV-infected blood and bloodstains were stored for up to 60 days at room temperature and subsequently screened for hepatitis B surface antigen (HBsAg) and HBV DNA. In addition, HBV-positive postmortem blood was added to a cell line and the production of HBV virions was examined over a period of 7 days. HBsAg and HBV DNA were detected in all samples stored for 60 days at room temperature. HBV-positive postmortem blood successfully infected the cell line and progeny viruses were produced for up to 6 days. Thus, it is crucial that due care is taken when handling not only living material infected with HBV, as well as other harmful viruses, but also blood or body fluids from cadavers or medical waste.
[Mh] Termos MeSH primário: DNA Viral/sangue
Anticorpos Anti-Hepatite B/sangue
Antígenos de Superfície da Hepatite B/sangue
Vírus da Hepatite B/isolamento & purificação
Hepatite B/sangue
[Mh] Termos MeSH secundário: Autopsia
Preservação de Sangue
Feminino
Células Hep G2
Hepatite B/prevenção & controle
Hepatite B/transmissão
Hepatite B/virologia
Vírus da Hepatite B/genética
Vírus da Hepatite B/imunologia
Seres Humanos
Masculino
Meia-Idade
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Hepatitis B Antibodies); 0 (Hepatitis B Surface Antigens)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171204
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3665-x


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[PMID]:28466544
[Au] Autor:Loza-Correa M; Kalab M; Yi QL; Eltringham-Smith LJ; Sheffield WP; Ramirez-Arcos S
[Ad] Endereço:Canadian Blood Services, Centre for Innovation, Ottawa, ON, Canada.
[Ti] Título:Comparison of bacterial attachment to platelet bags with and without preconditioning with plasma.
[So] Source:Vox Sang;112(5):401-407, 2017 Jul.
[Is] ISSN:1423-0410
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND OBJECTIVES: Canadian Blood Services produces apheresis and buffy coat pooled platelet concentrates (PCs) stored in bags produced by two different manufacturers (A and B, respectively), both made of polyvinyl chloride-butyryl trihexyl citrate. This study was aimed at comparing Staphylococcus epidermidis adhesion to the inner surface of both bag types in the presence or absence of plasma factors. MATERIALS AND METHODS: Sets (N = 2-6) of bags type A and B were left non-coated (control) or preconditioned with platelet-rich, platelet-poor or defibrinated plasma (PRP, PPP and DefibPPP, respectively). Each bag was inoculated with a 200-ml S. epidermidis culture adjusted to 0·5 colony-forming units/ml. Bags were incubated under platelet storage conditions for 7 days. After culture removal, bacteria attached to the plastic surface were either dislodged by sonication for bacterial quantification or examined in situ by scanning electron microscopy (SEM). RESULTS: Higher bacterial adhesion was observed to preconditioned PC bags than control containers for both bag types (P < 0·0001). Bacterial attachment to preconditioned bags was confirmed by SEM. Bacteria adhered equally to both types of containers in the presence of PRP, PPP and DefibPPP residues (P > 0·05). By contrast, a significant increase in bacterial adherence was observed to type A bags compared with type B bags in the absence of plasma (P < 0·05) [Correction added on 16 June 2017, after first online publication: this sentence has been corrected]. CONCLUSION: The ability of S. epidermidis to adhere to preconditioned platelet collection bags depends on the presence of plasma factors. Future efforts should be focused on reducing plasma proteins' attachment to platelet storage containers to decrease subsequent bacterial adhesion.
[Mh] Termos MeSH primário: Incrustação Biológica/prevenção & controle
Plaquetas
Preservação de Sangue/instrumentação
Staphylococcus epidermidis/fisiologia
[Mh] Termos MeSH secundário: Aderência Bacteriana
Materiais Revestidos Biocompatíveis
Seres Humanos
Plasma/química
Cloreto de Polivinila/química
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coated Materials, Biocompatible); 9002-86-2 (Polyvinyl Chloride)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1111/vox.12513


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[PMID]:29185590
[Au] Autor:Biagini S; Dale CS; Real JM; Moreira ES; Carvalho CRR; Schettino GPP; Wendel S; Azevedo LCP
[Ad] Endereço:Instituto de Ensino e Pesquisa, Hospital Sírio-Libanês, São Paulo, SP, Brasil.
[Ti] Título:Short-term effects of stored homologous red blood cell transfusion on cardiorespiratory function and inflammation: an experimental study in a hypovolemia model.
[So] Source:Braz J Med Biol Res;51(1):e6258, 2017 Nov 17.
[Is] ISSN:1414-431X
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The pathophysiological mechanisms associated with the effects of red blood cell (RBC) transfusion on cardiopulmonary function and inflammation are unclear. We developed an experimental model of homologous 14-days stored RBC transfusion in hypovolemic swine to evaluate the short-term effects of transfusion on cardiopulmonary system and inflammation. Sixteen healthy male anesthetized swine (68±3.3 kg) were submitted to controlled hemorrhage (25% of blood volume). Two units of non-filtered RBC from each animal were stored under blood bank conditions for 14 days. After 30 min of hypovolemia, the control group (n=8) received an infusion of lactated Ringer's solution (three times the removed volume). The transfusion group (n=8) received two units of homologous 14-days stored RBC and lactated Ringer's solution in a volume that was three times the difference between blood removed and blood transfusion infused. Both groups were followed up for 6 h after resuscitation with collection of hemodynamic and respiratory data. Cytokines and RNA expression were measured in plasma and lung tissue. Stored RBC transfusion significantly increased mixed oxygen venous saturation and arterial oxygen content. Transfusion was not associated with alterations on pulmonary function. Pulmonary concentrations of cytokines were not different between groups. Gene expression for lung cytokines demonstrated a 2-fold increase in mRNA level for inducible nitric oxide synthase and a 0.5-fold decrease in mRNA content for IL-21 in the transfused group. Thus, stored homologous RBC transfusion in a hypovolemia model improved cardiovascular parameters but did not induce significant effects on microcirculation, pulmonary inflammation and respiratory function up to 6 h after transfusion.
[Mh] Termos MeSH primário: Preservação de Sangue/métodos
Fenômenos Fisiológicos Cardiovasculares
Transfusão de Eritrócitos/métodos
Hipovolemia/terapia
Pneumonia/fisiopatologia
Fenômenos Fisiológicos Respiratórios
[Mh] Termos MeSH secundário: Animais
Preservação de Sangue/efeitos adversos
Citocinas/sangue
Modelos Animais de Doenças
Ensaio de Imunoadsorção Enzimática
Transfusão de Eritrócitos/efeitos adversos
Hemodinâmica
Masculino
Oxigênio/metabolismo
Reprodutibilidade dos Testes
Ressuscitação/métodos
Suínos
Fatores de Tempo
Resultado do Tratamento
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); S88TT14065 (Oxygen)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE


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[PMID]:27777340
[Au] Autor:Weisenhorn EM; van T Erve TJ; Riley NM; Hess JR; Raife TJ; Coon JJ
[Ad] Endereço:From the ‡Integrated Program in Biochemistry.
[Ti] Título:Multi-omics Evidence for Inheritance of Energy Pathways in Red Blood Cells.
[So] Source:Mol Cell Proteomics;15(12):3614-3623, 2016 Dec.
[Is] ISSN:1535-9484
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Each year over 90 million units of blood are transfused worldwide. Our dependence on this blood supply mandates optimized blood management and storage. During storage, red blood cells undergo degenerative processes resulting in altered metabolic characteristics which may make blood less viable for transfusion. However, not all stored blood spoils at the same rate, a difference that has been attributed to variable rates of energy usage and metabolism in red blood cells. Specific metabolite abundances are heritable traits; however, the link between heritability of energy metabolism and red blood cell storage profiles is unclear. Herein we performed a comprehensive metabolomics and proteomics study of red blood cells from 18 mono- and di-zygotic twin pairs to measure heritability and identify correlations with ATP and other molecular indices of energy metabolism. Without using affinity-based hemoglobin depletion, our work afforded the deepest multi-omic characterization of red blood cell membranes to date (1280 membrane proteins and 330 metabolites), with 119 membrane protein and 148 metabolite concentrations found to be over 30% heritable. We demonstrate a high degree of heritability in the concentration of energy metabolism metabolites, especially glycolytic metabolites. In addition to being heritable, proteins and metabolites involved in glycolysis and redox metabolism are highly correlated, suggesting that crucial energy metabolism pathways are inherited en bloc at distinct levels. We conclude that individuals can inherit a phenotype composed of higher or lower concentrations of these proteins together. This can result in vastly different red blood cells storage profiles which may need to be considered to develop precise and individualized storage options. Beyond guiding proper blood storage, this intimate link in heritability between energy and redox metabolism pathways may someday prove useful in determining the predisposition of an individual toward metabolic diseases.
[Mh] Termos MeSH primário: Eritrócitos/metabolismo
Proteínas de Membrana/metabolismo
Metabolômica/métodos
Proteômica/métodos
Característica Quantitativa Herdável
[Mh] Termos MeSH secundário: Adolescente
Adulto
Bancos de Sangue
Preservação de Sangue
Metabolismo Energético
Feminino
Seres Humanos
Masculino
Gêmeos Dizigóticos
Gêmeos Monozigóticos
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Membrane Proteins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171201
[Lr] Data última revisão:
171201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE


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[PMID]:28456565
[Au] Autor:Alshalani A; Acker JP
[Ad] Endereço:Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada; Department of Clinical Laboratory Sciences, King Saud University, Riyadh, Saudi Arabia.
[Ti] Título:Red blood cell membrane water permeability increases with length of ex vivo storage.
[So] Source:Cryobiology;76:51-58, 2017 Jun.
[Is] ISSN:1090-2392
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Water transport across the red blood cell (RBC) membrane is an essential cell function that needs to be preserved during ex vivo storage. Progressive biochemical depletion during storage can result in significant conformational and compositional changes to the membrane. Characterizing the changes to RBC water permeability can help in evaluating the quality of stored blood products and aid in the development of improved methods for the cryopreservation of red blood cells. This study aimed to characterize the water permeability (L ), osmotically inactive fraction (b), and Arrhenius activation energy (E ) at defined storage time-points throughout storage and to correlate the observed results with other in vitro RBC quality parameters. RBCs were collected from age- and sex-matched blood donors. A stopped flow spectrophotometer was used to determine L and b by monitoring changes in hemoglobin autofluorescence when RBCs were exposed to anisotonic solutions. Experimental values of L were characterized at three different temperatures (4, 20 and 37 °C) to determine the E . Results showed that L , b, and E of stored RBCs significantly increase by day 21 of storage. Degradation of the RBC membrane with length of storage was seen as an increase in hemolysis and supernatant potassium, and a decrease in deformability, mean corpuscular hemoglobin concentration and supernatant sodium. RBC osmotic characteristics were shown to change with storage and correlate with changes in RBC membrane quality metrics. Monitoring water parameters is a predictor of membrane damage and loss of membrane integrity in ex vivo stored RBCs.
[Mh] Termos MeSH primário: Preservação de Sangue/métodos
Eritrócitos
[Mh] Termos MeSH secundário: Permeabilidade da Membrana Celular
Hemólise
Seres Humanos
Masculino
Meia-Idade
Osmose
Água/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
059QF0KO0R (Water)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170501
[St] Status:MEDLINE


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[PMID]:28957448
[Au] Autor:Morishita Y; Nomura Y; Fukui C; Kawakami T; Ikeda T; Mukai T; Yuba T; Inamura KI; Yamaoka H; Miyazaki KI; Okazaki H; Haishima Y
[Ad] Endereço:Division of Medical Devices, National Institute of Health Sciences, Setagaya, Tokyo, Japan.
[Ti] Título:Pilot study on novel blood containers with alternative plasticizers for red cell concentrate storage.
[So] Source:PLoS One;12(9):e0185737, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Di (2-ethylhexyl) phthalate (DEHP), a typical plasticizer used for polyvinyl chloride (PVC) blood containers, is eluted from the blood containers and exerts protective effects on red blood cells. However, a concern for detrimental effects of DEHP on human health has led to the development of potential DEHP substitutes. Here, we compared the red blood cell preservation ability of two types of non-DEHP blood containers with safe alternative plasticizers to that of DEHP blood containers. Red cell concentrates in mannitol-adenine-phosphate solution (MAP/RCC) were stored for 6 weeks in PVC blood bags containing DEHP, di-isononyl-cyclohexane-1,2-dicarboxylate (DINCH) and di (2-ethylhexyl) 4-cyclohexene-1,2-dicarboxylate (DOTH), or 4-cyclohexene-1,2-dicarboxylic acid dinonyl ester (DL9TH) and DOTH. There was no significant difference in the total amount of plasticizer eluted into MAP/RCC (till 3 weeks from the beginning of the experiment), hemolysis of MAP/RCC, and osmotic fragility of MAP/RCC between the non-DEHP blood containers and DEHP blood containers. Hematological and blood chemical indices of MAP/RCC in all containers were nearly the same. Thus, DOTH/DINCH and DOTH/DL9TH blood containers demonstrate the same quality of MAP/RCC storing as the DEHP blood containers. Since DOTH, DINCH, and DL9TH were reported to be safe, DOTH/DINCH and DOTH/DL9TH blood containers are promising candidate substitutes for DEHP blood containers.
[Mh] Termos MeSH primário: Preservação de Sangue/métodos
Equipamentos e Provisões
Eritrócitos/metabolismo
Plastificantes/química
[Mh] Termos MeSH secundário: Seres Humanos
Projetos Piloto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plasticizers)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170929
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185737


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[PMID]:28926588
[Au] Autor:Huang LH; Lin PH; Tsai KW; Wang LJ; Huang YH; Kuo HC; Li SC
[Ad] Endereço:Genomics and Proteomics Core Laboratory, Department of Medical Research, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan.
[Ti] Título:The effects of storage temperature and duration of blood samples on DNA and RNA qualities.
[So] Source:PLoS One;12(9):e0184692, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:DNA and RNA samples from blood are the common examination target for non-invasive physical tests and/or biomedical studies. Since high-quality DNA and RNA samples guarantee the correctness of these tests and/or studies, we investigated the effects of storage temperature and storage duration of whole blood on DNA and RNA qualities. Subjects were enrolled to donate blood samples which were stored for different durations and at different temperatures, followed by the examinations on RNA quality, qPCR, DNA quality and DNA methylation. For RNA, we observed obvious quality decline with storage duration longer than 24 hours. Storage at low temperature does not keep RNA samples from degradation. And, storing whole blood samples in freezer dramatically damage RNA. For DNA, quality decline was not observed even with storage duration for 15 days. However, DNA methylation significantly altered with storage duration longer than three days. Storage duration within 24 hours is critical for collecting high-quality RNA samples for next-generation sequencing (NGS) assays (RIN≧8). If microarray assays are expected (RIN≧7), storage duration within 32 hours is acceptable. Although DNA is resistant within 15 days when kept in whole blood, DNA quantity dramatically decreases owing to WBC lysis. In addition, duration for more than three days significantly alter DNA methylation status, globally and locally. Our result provides a reference for dealing with blood samples.
[Mh] Termos MeSH primário: Preservação de Sangue
DNA/metabolismo
RNA/metabolismo
[Mh] Termos MeSH secundário: DNA/isolamento & purificação
Metilação de DNA
Sequenciamento de Nucleotídeos em Larga Escala
Seres Humanos
Leucócitos/citologia
Leucócitos/metabolismo
RNA/isolamento & purificação
Reação em Cadeia da Polimerase em Tempo Real
Análise de Sequência de DNA
Análise de Sequência de RNA
Temperatura Ambiente
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
63231-63-0 (RNA); 9007-49-2 (DNA)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170920
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184692


  10 / 10164 MEDLINE  
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[PMID]:28871619
[Au] Autor:Heddle NM
[Ad] Endereço:Department of Medicine, McMaster University, Hamilton, Ontario, Canada.
[Ti] Título:From cytokines to pragmatic designs: changing paradigms.
[So] Source:Transfusion;57(10):2298-2306, 2017 Oct.
[Is] ISSN:1537-2995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Emily Cooley was a well-respected medical technologist and morphologist with a remarkable skill set. She was highly regarded both professionally and personally. The "Emily Cooley Lectureship and Award" was established to honor her in particular and medical technologists in general. This article first reviews how a medical laboratory technologist was inspired to become a clinical researcher, then goes on to describe research that led to the discovery of cytokines as the cause of febrile nonhemolytic transfusion and the use of a pragmatic randomized controlled trial design to address evidence of harm when stored red blood cells were transfused. Important lessons for performing quality, meaningful research are highlighted.
[Mh] Termos MeSH primário: Citocinas
Reação Transfusional
[Mh] Termos MeSH secundário: Distinções e Prêmios
Pesquisa Biomédica
Preservação de Sangue
Febre/etiologia
História do Século XX
História do Século XXI
Seres Humanos
[Pt] Tipo de publicação:AUTOBIOGRAPHY; BIOGRAPHY; HISTORICAL ARTICLE; JOURNAL ARTICLE; LECTURES
[Ps] Nome de pessoa como assunto:Cooley E; Heddle N
[Nm] Nome de substância:
0 (Cytokines)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170906
[St] Status:MEDLINE
[do] DOI:10.1111/trf.14309



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